FvH4_1g17120

protein phosphatase 2C 78

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001678
Physical Location & Seq
Reverse (-)
9900154 .. 9903448
3295 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g17120.t1

Sequence Viewer

Length: 1122 bp
ATGATGTTGGAGATGTGTAGGAGGCCATTGGAGAAGTGCTTTGGAGGTGGAGATGATGAGCTTCTATGGCACATGGACTTGAAGCCTCACTATTCTGGGGAGTATTCTATTGCTGTTGTTCAAGCTAATTCAGCTTTGGAAGATCAGGGACAGGTGTTTACTTCTCCTTCTGCCACGTATGTTGGTGTTTACGATGGACATGGTGGTCCTGAAGCTTCTAGATTCATCACTCAGAGGCTATTCCCCTTTATTCAGAGGTTTTCAAATGAGCAAGGTGGACTATCAGAGGATGTGATTAGGAAAGCGTTTGATGCAACTGAGGAAGAGTTTATGGACTTGGTCAAGCAGTCATGGCGGACACGGCCAAGGATTGCTTCTGTTGGTTCTTGTTGTCTTGTTGGCGCAATTACGAATGATGTTTTGTATGTTGCTAATCTCGGAGATTCGAGGGCAGTTCTTGGTCGGAGAGCAATGGATGGGCCGACGGTGGTGGCAGAGAGGTTGTCTACGGATCACAATGTTGGAGTGGAGGAGGTGAGGAGGGAGGTTAAGGATCTTCACCCTGATGATTCACACATTGTGGTTTATACTCGTGGGGTTTGGCGAATTAAGGGCATTATACAGGTCTCTAGATCAATCGGAGATGTCTACTTGAAGAAACCTGAGTTTAACAGAGATCCGCTCTTCCGCTTCGGTATACCTGTTCCTTTAAAGAGGCCTGTAATGACAGCTGTACCCTCAATATTGGTTAGAAAATTGCGACCCGAGGATATGTTTTTGATATTTGCAACAGACGGTCTCTGGGAGCATTTGAGTGATGAAGCAGCTGTTAAAATCGTCTCAAAAAATCCAAGAGTTGGAATAGCAAAGCGACTGGTAAGAACTGCCATTGAGGAGGCTGCGAGAAAAAGAGAAATGAGATATGACGATATAAAGAAACTTGAAAAGGGGGTAAGGCGCCATTTCCACGATGATATCACTGTAATTGTGATATTCCTGGATCATACACAAACTTCCCTAAATGCTACAGATAACAGCCTTTTTACCTGCACTAGTGTTCCTGTTGATATCTTTTCAAATAATGATGATGAAGCAGTTGGCTCCCTTGATTCATTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

374

Amino Acids

42.01

Weight (kDa)

5.97

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 56 - 302 2.3e-39 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 204
Acc36I ACCTGC 1 cut(s) 1055
AccB1I GGYRCC 1 cut(s) 957
AccB7I CCANNNNNTGG 1 cut(s) 857
AccBSI CCGCTC 1 cut(s) 682
AccI GTMKAC 3 cut(s) 506, 648, 697
AciI CCGC 3 cut(s) 355, 680, 688
AclWI GGATC 4 cut(s) 519, 561, 671, 1008
AcoI YGGCCR 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 958
AdeI CACNNNGTG 1 cut(s) 580
AfaI GTAC 1 cut(s) 735
AfiI CCNNNNNNNGG 1 cut(s) 857
AgsI TTSAA 6 cut(s) 82, 122, 264, 655, 944, 1077
AhlI ACTAGT 1 cut(s) 1052
AjnI CCWGG 1 cut(s) 996
AjuI GAANNNNNNNTTGG 2 cut(s) 358, 390
AluBI AGCT 6 cut(s) 61, 125, 134, 215, 731, 827
AluI AGCT 6 cut(s) 61, 125, 134, 215, 731, 827
Alw26I GTCTC 3 cut(s) 631, 803, 844
AlwI GGATC 4 cut(s) 519, 561, 671, 1008
Ama87I CYCGRG 1 cut(s) 764
AoxI GGCC 4 cut(s) 23, 362, 479, 716
ApeKI GCWGC 2 cut(s) 824, 899
AspLEI GCGC 2 cut(s) 404, 960
AspS9I GGNCC 2 cut(s) 206, 479
AsuHPI GGTGA 2 cut(s) 547, 551
AvaI CYCGRG 1 cut(s) 764
AvaII GGWCC 1 cut(s) 206
BaeI ACNNNNGTAYC 2 cut(s) 717, 750
BanI GGYRCC 1 cut(s) 957
BauI CACGAG 1 cut(s) 591
BbvI GCAGC 2 cut(s) 836, 886
BccI CCATC 2 cut(s) 188, 470
BceAI ACGGC 1 cut(s) 377
BciT130I CCWGG 1 cut(s) 998
BcoDI GTCTC 3 cut(s) 631, 803, 844
BcuI ACTAGT 1 cut(s) 1052
BfaI CTAG 3 cut(s) 219, 630, 1053
BfmI CTRYAG 1 cut(s) 1026
BfoI RGCGCY 1 cut(s) 961
BfuAI ACCTGC 1 cut(s) 1055
BisI GCNGC 2 cut(s) 825, 900
BlsI GCNGC 2 cut(s) 826, 901
Bme1390I CCNGG 1 cut(s) 998
Bme18I GGWCC 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 764
BmgT120I GGNCC 2 cut(s) 206, 479
BmiI GGNNCC 2 cut(s) 959, 1102
BmrFI CCNGG 1 cut(s) 998
BmsI GCATC 1 cut(s) 301
BplI GAGNNNNNCTC 2 cut(s) 666, 698
BsaAI YACGTR 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 958
BsaI GGTCTC 2 cut(s) 631, 803
BsaJI CCNNGG 2 cut(s) 365, 765
Bsc4I CCNNNNNNNGG 1 cut(s) 857
Bse1I ACTGG 1 cut(s) 879
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 998
BseDI CCNNGG 2 cut(s) 365, 765
BseGI GGATG 2 cut(s) 295, 481
BseLI CCNNNNNNNGG 1 cut(s) 857
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 3 cut(s) 245, 309, 654
BseNI ACTGG 1 cut(s) 879
BseRI GAGGAG 3 cut(s) 545, 553, 908
BseXI GCAGC 2 cut(s) 836, 886
BsgI GTGCAG 1 cut(s) 1033
BshFI GGCC 4 cut(s) 25, 364, 481, 718
BshNI GGYRCC 1 cut(s) 957
BsiHKCI CYCGRG 1 cut(s) 764
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 857
BsmAI GTCTC 3 cut(s) 631, 803, 844
BsmBI CGTCTC 1 cut(s) 844
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 4 cut(s) 25, 364, 481, 718
Bso31I GGTCTC 2 cut(s) 631, 803
BsoBI CYCGRG 1 cut(s) 764
Bsp143I GATC 6 cut(s) 142, 511, 553, 632, 676, 1000
BspACI CCGC 3 cut(s) 355, 680, 688
BspANI GGCC 4 cut(s) 25, 364, 481, 718
BspCNI CTCAG 3 cut(s) 244, 310, 655
BspLI GGNNCC 2 cut(s) 959, 1102
BspMI ACCTGC 1 cut(s) 1055
BspPI GGATC 4 cut(s) 519, 561, 671, 1008
BspQI GCTCTTC 1 cut(s) 689
BspT107I GGYRCC 1 cut(s) 957
BspTNI GGTCTC 2 cut(s) 631, 803
BsrBI CCGCTC 1 cut(s) 682
BsrDI GCAATG 1 cut(s) 477
BsrI ACTGG 1 cut(s) 879
BssECI CCNNGG 2 cut(s) 365, 765
BssMI GATC 6 cut(s) 142, 511, 553, 632, 676, 1000
BssNAI GTATAC 1 cut(s) 698
BssNI GRCGYC 1 cut(s) 958
BssSI CACGAG 1 cut(s) 591
BssT1I CCWWGG 1 cut(s) 365
Bst1107I GTATAC 1 cut(s) 698
Bst2BI CACGAG 1 cut(s) 591
Bst2UI CCWGG 1 cut(s) 998
Bst4CI ACNGT 3 cut(s) 487, 797, 982
Bst6I CTCTTC 2 cut(s) 318, 689
BstACI GRCGYC 1 cut(s) 958
BstBAI YACGTR 1 cut(s) 177
BstDEI CTNAG 3 cut(s) 231, 318, 663
BstF5I GGATG 2 cut(s) 295, 481
BstH2I RGCGCY 1 cut(s) 961
BstHHI GCGC 2 cut(s) 404, 960
BstKTI GATC 6 cut(s) 145, 514, 556, 635, 679, 1003
BstMAI GTCTC 3 cut(s) 631, 803, 844
BstMBI GATC 6 cut(s) 142, 511, 553, 632, 676, 1000
BstMWI GCNNNNNNNGC 5 cut(s) 67, 131, 311, 352, 361
BstNI CCWGG 1 cut(s) 998
BstSCI CCNGG 1 cut(s) 996
BstSFI CTRYAG 1 cut(s) 1026
BstV1I GCAGC 2 cut(s) 836, 886
BstX2I RGATCY 2 cut(s) 553, 676
BstYI RGATCY 2 cut(s) 553, 676
BstZ17I GTATAC 1 cut(s) 698
BsuRI GGCC 4 cut(s) 25, 364, 481, 718
BtsCI GGATG 2 cut(s) 295, 481
BtsIMutI CAGTG 1 cut(s) 978
BveI ACCTGC 1 cut(s) 1055
CfoI GCGC 2 cut(s) 404, 960
Cfr13I GGNCC 2 cut(s) 206, 479
Csp6I GTAC 1 cut(s) 734
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 3 cut(s) 73, 200, 351
CviQI GTAC 1 cut(s) 734
DdeI CTNAG 3 cut(s) 231, 318, 663
DinI GGCGCC 1 cut(s) 959
DpnI GATC 6 cut(s) 144, 513, 555, 634, 678, 1002
DpnII GATC 6 cut(s) 142, 511, 553, 632, 676, 1000
DraI TTTAAA 1 cut(s) 711
DraIII CACNNNGTG 1 cut(s) 580
DrdI GACNNNNNNGTC 1 cut(s) 204
DseDI GACNNNNNNGTC 1 cut(s) 204
EaeI YGGCCR 1 cut(s) 362
Eam1104I CTCTTC 2 cut(s) 318, 689
EarI CTCTTC 2 cut(s) 318, 689
EciI GGCGGA 1 cut(s) 370
Eco130I CCWWGG 1 cut(s) 365
Eco147I AGGCCT 1 cut(s) 718
Eco31I GGTCTC 2 cut(s) 631, 803
Eco32I GATATC 2 cut(s) 976, 1069
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 764
EcoRII CCWGG 1 cut(s) 996
EcoRV GATATC 2 cut(s) 976, 1069
EcoT14I CCWWGG 1 cut(s) 365
EgeI GGCGCC 1 cut(s) 959
EheI GGCGCC 1 cut(s) 959
ErhI CCWWGG 1 cut(s) 365
Esp3I CGTCTC 1 cut(s) 844
FaeI CATG 3 cut(s) 76, 203, 354
FalI AAGNNNNNCTT 2 cut(s) 358, 390
FaqI GGGAC 1 cut(s) 162
FatI CATG 3 cut(s) 72, 199, 350
FblI GTMKAC 3 cut(s) 506, 648, 697
Fnu4HI GCNGC 2 cut(s) 825, 900
FokI GGATG 2 cut(s) 302, 488
Fsp4HI GCNGC 2 cut(s) 825, 900
FspBI CTAG 3 cut(s) 219, 630, 1053
GlaI GCGC 2 cut(s) 403, 959
GluI GCNGC 2 cut(s) 825, 900
HaeII RGCGCY 1 cut(s) 961
HaeIII GGCC 4 cut(s) 25, 364, 481, 718
HhaI GCGC 2 cut(s) 404, 960
Hin1I GRCGYC 1 cut(s) 958
Hin1II CATG 3 cut(s) 76, 203, 354
Hin6I GCGC 2 cut(s) 402, 958
HinP1I GCGC 2 cut(s) 402, 958
HindIII AAGCTT 1 cut(s) 213
HinfI GANTC 4 cut(s) 222, 443, 569, 1109
HphI GGTGA 2 cut(s) 547, 551
Hpy166II GTNNAC 6 cut(s) 159, 190, 278, 507, 649, 698
Hpy188I TCNGA 6 cut(s) 234, 255, 286, 440, 465, 641
Hpy188III TCNNGA 3 cut(s) 209, 219, 630
Hpy8I GTNNAC 6 cut(s) 159, 190, 278, 507, 649, 698
Hpy99I CGWCG 1 cut(s) 487
HpyAV CCTTC 1 cut(s) 177
HpyCH4III ACNGT 3 cut(s) 487, 797, 982
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 3 cut(s) 314, 788, 1050
HpyF10VI GCNNNNNNNGC 5 cut(s) 67, 131, 311, 352, 361
HpyF3I CTNAG 3 cut(s) 231, 318, 663
HpySE526I ACGT 1 cut(s) 176
Hsp92I GRCGYC 1 cut(s) 958
Hsp92II CATG 3 cut(s) 76, 203, 354
HspAI GCGC 2 cut(s) 402, 958
KasI GGCGCC 1 cut(s) 957
Kzo9I GATC 6 cut(s) 142, 511, 553, 632, 676, 1000
LguI GCTCTTC 1 cut(s) 689
LmnI GCTCC 2 cut(s) 805, 1106
Lsp1109I GCAGC 2 cut(s) 836, 886
LweI GCATC 1 cut(s) 301
MaeI CTAG 3 cut(s) 219, 630, 1053
MaeII ACGT 1 cut(s) 176
MalI GATC 6 cut(s) 144, 513, 555, 634, 678, 1002
MbiI CCGCTC 1 cut(s) 682
MboI GATC 6 cut(s) 142, 511, 553, 632, 676, 1000
MboII GAAGA 5 cut(s) 152, 335, 548, 667, 676
MflI RGATCY 2 cut(s) 553, 676
MluCI AATT 5 cut(s) 127, 405, 606, 755, 984
Mly113I GGCGCC 1 cut(s) 958
MmeI TCCRAC 3 cut(s) 443, 502, 838
MseI TTAA 5 cut(s) 549, 609, 669, 710, 831
MslI CAYNNNNRTG 2 cut(s) 564, 813
MspA1I CMGCKG 2 cut(s) 731, 827
MspR9I CCNGG 1 cut(s) 998
MvaI CCWGG 1 cut(s) 998
MwoI GCNNNNNNNGC 5 cut(s) 67, 131, 311, 352, 361
NarI GGCGCC 1 cut(s) 958
NdeII GATC 6 cut(s) 142, 511, 553, 632, 676, 1000
NlaIII CATG 3 cut(s) 76, 203, 354
NlaIV GGNNCC 2 cut(s) 959, 1102
PceI AGGCCT 1 cut(s) 718
PciSI GCTCTTC 1 cut(s) 689
PfeI GAWTC 4 cut(s) 222, 443, 569, 1109
PflFI GACNNNGTC 1 cut(s) 338
PflMI CCANNNNNTGG 1 cut(s) 857
PfoI TCCNGGA 1 cut(s) 996
PkrI GCNGC 2 cut(s) 826, 901
PluTI GGCGCC 1 cut(s) 961
Ppu21I YACGTR 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 996
PspGI CCWGG 1 cut(s) 996
PspN4I GGNNCC 2 cut(s) 959, 1102
PspPI GGNCC 2 cut(s) 206, 479
PsuI RGATCY 2 cut(s) 553, 676
PsyI GACNNNGTC 1 cut(s) 338
PvuII CAGCTG 2 cut(s) 731, 827
RsaI GTAC 1 cut(s) 735
RsaNI GTAC 1 cut(s) 734
RseI CAYNNNNRTG 2 cut(s) 564, 813
SapI GCTCTTC 1 cut(s) 689
SaqAI TTAA 5 cut(s) 549, 609, 669, 710, 831
SatI GCNGC 2 cut(s) 825, 900
Sau3AI GATC 6 cut(s) 142, 511, 553, 632, 676, 1000
Sau96I GGNCC 2 cut(s) 206, 479
ScrFI CCNGG 1 cut(s) 998
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 1026
SfoI GGCGCC 1 cut(s) 959
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 564, 813
SpeI ACTAGT 1 cut(s) 1052
Sse9I AATT 5 cut(s) 127, 405, 606, 755, 984
SseBI AGGCCT 1 cut(s) 718
SsiI CCGC 3 cut(s) 355, 680, 688
SspDI GGCGCC 1 cut(s) 957
SspI AATATT 1 cut(s) 744
SspMI CTAG 3 cut(s) 219, 630, 1053
StuI AGGCCT 1 cut(s) 718
StyD4I CCNGG 1 cut(s) 996
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 3 cut(s) 487, 797, 982
TaiI ACGT 1 cut(s) 179
TaqI TCGA 1 cut(s) 446
TasI AATT 5 cut(s) 127, 405, 606, 755, 984
TfiI GAWTC 4 cut(s) 222, 443, 569, 1109
Tru1I TTAA 5 cut(s) 549, 609, 669, 710, 831
Tru9I TTAA 5 cut(s) 549, 609, 669, 710, 831
TscAI CASTG 1 cut(s) 985
TseI GCWGC 2 cut(s) 824, 899
TspDTI ATGAA 4 cut(s) 214, 834, 1101, 1104
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 1 cut(s) 985
Tth111I GACNNNGTC 1 cut(s) 338
Van91I CCANNNNNTGG 1 cut(s) 857
VpaK11BI GGWCC 1 cut(s) 206
XbaI TCTAGA 2 cut(s) 218, 629
XmiI GTMKAC 3 cut(s) 506, 648, 697
XspI CTAG 3 cut(s) 219, 630, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.