Rw2G015230

protein phosphatase 2C 78

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001678
Physical Location & Seq
Reverse (-)
17582303 .. 17584757
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G015230.1

Sequence Viewer

Length: 1119 bp
ATGTTGGAGATGTGTAGGAGACCATTGGAGAAGTGCTTTGGAGGTGGAGATGATGAGCTTCTATGGCACACGGACTTGAAGCCTCACTCTTCTGGGGAGTATTCAATTGCTGTTGTTCAGGCTAATTCATCTTTGGAAGATCAGGGACAAGTGTTCACTTCTCCTTCTGCCACGTATGTTGGAGTTTATGACGGCCATGGTGGTCCTGAAGCTTCTAGATTCATCACTCAGCGGCTCTTCCCCTATATTCAGAAGTTTTCAAATGAGCAAGGTGGACTATCAGAGGATGTGATTAGGAAAGCGTTTGATGCAACTGAGGAGGAGTTTGTGGACTTGGTCAAGCGGTCATGGCCGGCACGGCCAAGCATTGCTTCTGTAGGTTCATGCTGTCTTGTTGGCGCCATTACGAATGATGTCTTGTATGTGGCTAATCTCGGAGATTCGAGGGCGGTTCTTGGTCGGAGAGCAATAGATGGACCGACGGTGGTGGCAGAGCGGTTGTCTACAGATCACAATGTTGGAGTGGAGGAGGTGAGGAGGGAGGTTAAGGATCTGCACCCTGACGATTCGCACATTGTGGTTTATACTCGTGGGGTTTGGCGAATTAAGGGCATTATTCAGGTCTCTAGATCAATCGGAGATGTCTACTTGAAGAAACCTGAGTTTAACAGAGATCCTCTCTTCCAGTTTGGTATACCTGTTCCTTTAAAGAGGCCTGTAATGACATCAGTACCCTCAATATTGGTTCGGAAGTTGCAACCCCAGGACATGTTTTTGATATTTGCAACAGATGGTCTCTGGGAGCATTTGACTGATGAAGCAGCTGTTAAAGTCGTCTCAAAAAATTCAAGAGTTGGAATAGCAAAGCGACTGGTAAGAACTGCCATTGAGGAGGCTGCAAGGAAAAGAGAATTGAGATATGATGACATAAAGAAAGTTGAAAAGGGGGTAAGGCGTCATTTCCATGATGATATCACTGTAATTGTGATATTCCTGGATCATCCACAAACTTCCCCAAATGCTACGGATAACAGCCTCTTTACCTGCACTAGTGTTCCCGTTGATATCTTTTCTAATAATGATGATGAAGCAGTTGGCTCACTTCATACATTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

372

Amino Acids

41.53

Weight (kDa)

5.91

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 55 - 301 1.5e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1052
AccB1I GGYRCC 1 cut(s) 398
AccBSI CCGCTC 1 cut(s) 496
AccI GTMKAC 3 cut(s) 503, 645, 694
AciI CCGC 4 cut(s) 232, 343, 449, 496
AclWI GGATC 3 cut(s) 558, 668, 1005
AcoI YGGCCR 3 cut(s) 193, 350, 359
AcsI RAATTY 1 cut(s) 844
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 2 cut(s) 399, 955
AdeI CACNNNGTG 1 cut(s) 577
AfaI GTAC 1 cut(s) 732
AflIII ACRYGT 1 cut(s) 768
AgsI TTSAA 6 cut(s) 79, 105, 261, 652, 849, 941
AhlI ACTAGT 1 cut(s) 1049
AjnI CCWGG 2 cut(s) 762, 993
AjuI GAANNNNNNNTTGG 2 cut(s) 355, 387
AluBI AGCT 3 cut(s) 58, 212, 824
AluI AGCT 3 cut(s) 58, 212, 824
Alw26I GTCTC 4 cut(s) 13, 628, 800, 841
AlwI GGATC 3 cut(s) 558, 668, 1005
AoxI GGCC 4 cut(s) 193, 350, 359, 713
ApeKI GCWGC 2 cut(s) 821, 896
ApoI RAATTY 1 cut(s) 844
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 2 cut(s) 203, 476
AsuHPI GGTGA 1 cut(s) 544
AvaII GGWCC 2 cut(s) 203, 476
BaeI ACNNNNGTAYC 2 cut(s) 714, 747
BanI GGYRCC 1 cut(s) 398
BauI CACGAG 1 cut(s) 588
BbvI GCAGC 2 cut(s) 833, 883
BccI CCATC 2 cut(s) 467, 785
BceAI ACGGC 2 cut(s) 208, 374
BciT130I CCWGG 2 cut(s) 764, 995
BcoDI GTCTC 4 cut(s) 13, 628, 800, 841
BcuI ACTAGT 1 cut(s) 1049
BfaI CTAG 3 cut(s) 216, 627, 1050
BfmI CTRYAG 2 cut(s) 375, 504
BfoI RGCGCY 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 1052
BglI GCCNNNNNGGC 1 cut(s) 358
BisI GCNGC 3 cut(s) 233, 822, 897
BlsI GCNGC 3 cut(s) 234, 823, 898
Bme1390I CCNGG 2 cut(s) 764, 995
Bme18I GGWCC 2 cut(s) 203, 476
BmgT120I GGNCC 2 cut(s) 203, 476
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 2 cut(s) 764, 995
BmsI GCATC 1 cut(s) 298
BplI GAGNNNNNCTC 2 cut(s) 663, 695
BsaAI YACGTR 1 cut(s) 174
BsaHI GRCGYC 2 cut(s) 399, 955
BsaI GGTCTC 3 cut(s) 13, 628, 800
BsaJI CCNNGG 2 cut(s) 196, 762
Bse118I RCCGGY 1 cut(s) 352
Bse1I ACTGG 2 cut(s) 685, 876
Bse3DI GCAATG 1 cut(s) 366
BseBI CCWGG 2 cut(s) 764, 995
BseDI CCNNGG 2 cut(s) 196, 762
BseGI GGATG 2 cut(s) 292, 1000
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 3 cut(s) 242, 306, 651
BseNI ACTGG 2 cut(s) 685, 876
BseRI GAGGAG 5 cut(s) 332, 335, 542, 550, 905
BseXI GCAGC 2 cut(s) 833, 883
BsgI GTGCAG 2 cut(s) 539, 1030
BshFI GGCC 4 cut(s) 195, 352, 361, 715
BshNI GGYRCC 1 cut(s) 398
BsiSI CCGG 1 cut(s) 353
BslFI GGGAC 1 cut(s) 159
BsmAI GTCTC 4 cut(s) 13, 628, 800, 841
BsmBI CGTCTC 1 cut(s) 841
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 4 cut(s) 195, 352, 361, 715
Bso31I GGTCTC 3 cut(s) 13, 628, 800
Bsp143I GATC 6 cut(s) 139, 508, 550, 629, 673, 997
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 4 cut(s) 232, 343, 449, 496
BspANI GGCC 4 cut(s) 195, 352, 361, 715
BspCNI CTCAG 3 cut(s) 241, 307, 652
BspLI GGNNCC 1 cut(s) 400
BspMI ACCTGC 1 cut(s) 1052
BspPI GGATC 3 cut(s) 558, 668, 1005
BspQI GCTCTTC 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 398
BspTNI GGTCTC 3 cut(s) 13, 628, 800
BsrBI CCGCTC 1 cut(s) 496
BsrDI GCAATG 1 cut(s) 366
BsrFI RCCGGY 1 cut(s) 352
BsrI ACTGG 2 cut(s) 685, 876
BssAI RCCGGY 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 196, 762
BssMI GATC 6 cut(s) 139, 508, 550, 629, 673, 997
BssNAI GTATAC 1 cut(s) 695
BssNI GRCGYC 2 cut(s) 399, 955
BssSI CACGAG 1 cut(s) 588
BssT1I CCWWGG 1 cut(s) 196
Bst1107I GTATAC 1 cut(s) 695
Bst2BI CACGAG 1 cut(s) 588
Bst2UI CCWGG 2 cut(s) 764, 995
Bst4CI ACNGT 2 cut(s) 484, 979
Bst6I CTCTTC 3 cut(s) 94, 242, 686
BstACI GRCGYC 2 cut(s) 399, 955
BstBAI YACGTR 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 3 cut(s) 228, 315, 660
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 2 cut(s) 292, 1000
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 6 cut(s) 142, 511, 553, 632, 676, 1000
BstMAI GTCTC 4 cut(s) 13, 628, 800, 841
BstMBI GATC 6 cut(s) 139, 508, 550, 629, 673, 997
BstMWI GCNNNNNNNGC 4 cut(s) 64, 308, 349, 358
BstNI CCWGG 2 cut(s) 764, 995
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 2 cut(s) 762, 993
BstSFI CTRYAG 2 cut(s) 375, 504
BstV1I GCAGC 2 cut(s) 833, 883
BstX2I RGATCY 2 cut(s) 550, 673
BstYI RGATCY 2 cut(s) 550, 673
BstZ17I GTATAC 1 cut(s) 695
BsuRI GGCC 4 cut(s) 195, 352, 361, 715
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 2 cut(s) 292, 1000
BtsIMutI CAGTG 1 cut(s) 975
BveI ACCTGC 1 cut(s) 1052
Cac8I GCNNGC 1 cut(s) 354
CfoI GCGC 1 cut(s) 401
Cfr10I RCCGGY 1 cut(s) 352
Cfr13I GGNCC 2 cut(s) 203, 476
CseI GACGC 1 cut(s) 944
Csp6I GTAC 1 cut(s) 731
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 5 cut(s) 197, 348, 384, 769, 965
CviQI GTAC 1 cut(s) 731
DdeI CTNAG 3 cut(s) 228, 315, 660
DinI GGCGCC 1 cut(s) 400
DpnI GATC 6 cut(s) 141, 510, 552, 631, 675, 999
DpnII GATC 6 cut(s) 139, 508, 550, 629, 673, 997
DraI TTTAAA 1 cut(s) 708
DraIII CACNNNGTG 1 cut(s) 577
EaeI YGGCCR 3 cut(s) 193, 350, 359
Eam1104I CTCTTC 3 cut(s) 94, 242, 686
EarI CTCTTC 3 cut(s) 94, 242, 686
Eco130I CCWWGG 1 cut(s) 196
Eco147I AGGCCT 1 cut(s) 715
Eco31I GGTCTC 3 cut(s) 13, 628, 800
Eco32I GATATC 2 cut(s) 973, 1066
Eco47I GGWCC 2 cut(s) 203, 476
Eco57I CTGAAG 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 762, 993
EcoRV GATATC 2 cut(s) 973, 1066
EcoT14I CCWWGG 1 cut(s) 196
EgeI GGCGCC 1 cut(s) 400
EheI GGCGCC 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 841
FaeI CATG 5 cut(s) 200, 351, 387, 772, 968
FalI AAGNNNNNCTT 2 cut(s) 355, 387
FaqI GGGAC 1 cut(s) 159
FatI CATG 5 cut(s) 196, 347, 383, 768, 964
FblI GTMKAC 3 cut(s) 503, 645, 694
Fnu4HI GCNGC 3 cut(s) 233, 822, 897
FokI GGATG 2 cut(s) 299, 987
Fsp4HI GCNGC 3 cut(s) 233, 822, 897
FspBI CTAG 3 cut(s) 216, 627, 1050
GlaI GCGC 1 cut(s) 400
GluI GCNGC 3 cut(s) 233, 822, 897
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 4 cut(s) 195, 352, 361, 715
HapII CCGG 1 cut(s) 353
HgaI GACGC 1 cut(s) 944
HhaI GCGC 1 cut(s) 401
Hin1I GRCGYC 2 cut(s) 399, 955
Hin1II CATG 5 cut(s) 200, 351, 387, 772, 968
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 3 cut(s) 219, 440, 566
HpaII CCGG 1 cut(s) 353
HphI GGTGA 1 cut(s) 544
Hpy166II GTNNAC 6 cut(s) 156, 275, 331, 504, 646, 695
Hpy188I TCNGA 6 cut(s) 252, 283, 437, 462, 638, 750
Hpy188III TCNNGA 4 cut(s) 206, 216, 627, 849
Hpy8I GTNNAC 6 cut(s) 156, 275, 331, 504, 646, 695
Hpy99I CGWCG 1 cut(s) 484
HpyAV CCTTC 1 cut(s) 174
HpyCH4III ACNGT 2 cut(s) 484, 979
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 6 cut(s) 311, 556, 757, 785, 899, 1047
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 308, 349, 358
HpyF3I CTNAG 3 cut(s) 228, 315, 660
HpySE526I ACGT 1 cut(s) 173
Hsp92I GRCGYC 2 cut(s) 399, 955
Hsp92II CATG 5 cut(s) 200, 351, 387, 772, 968
HspAI GCGC 1 cut(s) 399
KasI GGCGCC 1 cut(s) 398
KroI GCCGGC 1 cut(s) 352
KroNI GCCGGC 1 cut(s) 354
Kzo9I GATC 6 cut(s) 139, 508, 550, 629, 673, 997
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 1 cut(s) 802
Lsp1109I GCAGC 2 cut(s) 833, 883
LweI GCATC 1 cut(s) 298
MaeI CTAG 3 cut(s) 216, 627, 1050
MaeII ACGT 1 cut(s) 173
MalI GATC 6 cut(s) 141, 510, 552, 631, 675, 999
MbiI CCGCTC 1 cut(s) 496
MboI GATC 6 cut(s) 139, 508, 550, 629, 673, 997
MboII GAAGA 5 cut(s) 81, 149, 229, 664, 673
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 2 cut(s) 550, 673
MluCI AATT 6 cut(s) 105, 124, 603, 844, 911, 981
Mly113I GGCGCC 1 cut(s) 399
MmeI TCCRAC 4 cut(s) 160, 440, 499, 835
MroNI GCCGGC 1 cut(s) 352
MseI TTAA 5 cut(s) 546, 606, 666, 707, 828
MslI CAYNNNNRTG 1 cut(s) 963
MspA1I CMGCKG 2 cut(s) 232, 824
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 2 cut(s) 764, 995
MunI CAATTG 1 cut(s) 105
MvaI CCWGG 2 cut(s) 764, 995
MwoI GCNNNNNNNGC 4 cut(s) 64, 308, 349, 358
NaeI GCCGGC 1 cut(s) 354
NarI GGCGCC 1 cut(s) 399
NcoI CCATGG 1 cut(s) 196
NdeII GATC 6 cut(s) 139, 508, 550, 629, 673, 997
NgoMIV GCCGGC 1 cut(s) 352
NlaIII CATG 5 cut(s) 200, 351, 387, 772, 968
NlaIV GGNNCC 1 cut(s) 400
NspI RCATGY 1 cut(s) 772
PceI AGGCCT 1 cut(s) 715
PciI ACATGT 1 cut(s) 768
PciSI GCTCTTC 1 cut(s) 242
PdiI GCCGGC 1 cut(s) 354
PfeI GAWTC 3 cut(s) 219, 440, 566
PflFI GACNNNGTC 1 cut(s) 335
PfoI TCCNGGA 1 cut(s) 993
PkrI GCNGC 3 cut(s) 234, 823, 898
PluTI GGCGCC 1 cut(s) 402
Ppu21I YACGTR 1 cut(s) 174
PscI ACATGT 1 cut(s) 768
Psp6I CCWGG 2 cut(s) 762, 993
PspGI CCWGG 2 cut(s) 762, 993
PspN4I GGNNCC 1 cut(s) 400
PspPI GGNCC 2 cut(s) 203, 476
PsuI RGATCY 2 cut(s) 550, 673
PsyI GACNNNGTC 1 cut(s) 335
PvuII CAGCTG 1 cut(s) 824
RsaI GTAC 1 cut(s) 732
RsaNI GTAC 1 cut(s) 731
RseI CAYNNNNRTG 1 cut(s) 963
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 5 cut(s) 546, 606, 666, 707, 828
SatI GCNGC 3 cut(s) 233, 822, 897
Sau3AI GATC 6 cut(s) 139, 508, 550, 629, 673, 997
Sau96I GGNCC 2 cut(s) 203, 476
ScrFI CCNGG 2 cut(s) 764, 995
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 2 cut(s) 375, 504
SfiI GGCCNNNNNGGCC 1 cut(s) 358
SfoI GGCGCC 1 cut(s) 400
SinI GGWCC 2 cut(s) 203, 476
SmiMI CAYNNNNRTG 1 cut(s) 963
SpeI ACTAGT 1 cut(s) 1049
Sse9I AATT 6 cut(s) 105, 124, 603, 844, 911, 981
SseBI AGGCCT 1 cut(s) 715
SsiI CCGC 4 cut(s) 232, 343, 449, 496
SspDI GGCGCC 1 cut(s) 398
SspI AATATT 1 cut(s) 741
SspMI CTAG 3 cut(s) 216, 627, 1050
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 2 cut(s) 762, 993
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 2 cut(s) 484, 979
TaiI ACGT 1 cut(s) 176
TaqI TCGA 1 cut(s) 443
TaqII GACCGA 1 cut(s) 493
TasI AATT 6 cut(s) 105, 124, 603, 844, 911, 981
TauI GCSGC 1 cut(s) 235
TfiI GAWTC 3 cut(s) 219, 440, 566
Tru1I TTAA 5 cut(s) 546, 606, 666, 707, 828
Tru9I TTAA 5 cut(s) 546, 606, 666, 707, 828
TscAI CASTG 1 cut(s) 982
TseI GCWGC 2 cut(s) 821, 896
TspDTI ATGAA 6 cut(s) 117, 211, 372, 831, 1094, 1101
TspGWI ACGGA 2 cut(s) 86, 1040
TspRI CASTG 1 cut(s) 982
Tth111I GACNNNGTC 1 cut(s) 335
VpaK11BI GGWCC 2 cut(s) 203, 476
XapI RAATTY 1 cut(s) 844
XbaI TCTAGA 2 cut(s) 215, 626
XceI RCATGY 1 cut(s) 772
XmiI GTMKAC 3 cut(s) 503, 645, 694
XspI CTAG 3 cut(s) 216, 627, 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.