AT5G19300

methyltransferase C9orf114

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003646
Physical Location & Seq
Forward (+)
6495434 .. 6498332
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G19300.1

Sequence Viewer

Length: 1197 bp
ATGGGGAAGAAGAACAAGAGAAGTCAAGACGAGTCTGAGCTCGAATTGGAGCCAGAGCTAACGAAAATAATCGATGGAGACTCTAAAAAGAAGAAAAATAAGAATAAGAAGAAGAGAAGCCATGAAGATACGGAGATAGAACCGGAGCAAAAGATGAGTCTCGACGGAGACTCGAAGGAGGAGAAGATAAAGAAGAAGAGGAAGAACAAGAACCAAGAGGAGGAGCCAGAGCTTGTGACGGAGAAAACGAAAGTCCAAGAGGAGGAAAAGGGAAATGTAGAAGAGGGTAGAGCCACTGTTAGCATAGCCATAGCTGGTTCAATCATCCACAACACTCAATCACTTGAGCTCGCCACACGCCTCGCCGGCCAAATTGCTCGTGCAGCTACAATTTTCCGAATCGACGAGATCGTAGTGTTCGACAATAAGAGCAGCTCAGAAATCGAATCAGCTGCTACGAATGCTTCTGATAGCAATGAAAGTGGTGCCTCCTTTCTCGTTCGTATCTTGAAGTATCTAGAGACACCACAATATTTGAGGAAATCTCTCTTCCCCAAGCAAAATGATCTTAGATATGTGGGTATGTTGCCACCTCTTGATGCTCCTCACCATCTGCGTAAGCACGAGTGGGAACAATACCGTGAAGGTGTGACACTGAGTGAAAAGGCTCCAAATTCTGAGGGTACATTGGTTGATGTGGGTTTAAGCAAGAGTGTTGTTGTTGATCAAGTGCTGGGTCCAGGAATTAGAGTCACGGTAGCCATGGGAACTGACCACGATTTAGATTTGGTACGCCAGATTGTTCCACCCTCTAAGCCAAGGGAAGAAGCAGGAATGTATTGGGGATACAAAGTACGATATGCATCACAATTAAGTTCAGTATTCAAGGAATGCCCTTTCGAGGGTGGTTACGATTATTTGATTGGTACCTCGGAGCACGGCCTGGTAATTAGTTCATCTGAGCTGAAAATACCAACATTTAGGCACCTATTGATTGCATTTGGTGGACTTGCTGGGCTTGAAGAAAGTATTGAAGATGATAATCAGTATAAGGGGAAAAACGTTCGAGATGTGTTTAATGTATACTTGAATACTTGTCCACATCAAGGTAGCCGAACCATTCGAGCAGAGGAAGCGATGTTTATATCACTTCAGTACTTCCAGGAACCCATCAGCAGGGCAGTGAGAAGACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

398

Amino Acids

44.88

Weight (kDa)

6.09

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 99 - 386 2.6e-110 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 926
AccB1I GGYRCC 3 cut(s) 485, 926, 984
AccI GTMKAC 1 cut(s) 1083
AclI AACGTT 1 cut(s) 1062
AcoI YGGCCR 1 cut(s) 367
AcsI RAATTY 1 cut(s) 673
AcuI CTGAAG 1 cut(s) 1136
AdeI CACNNNGTG 1 cut(s) 659
AfaI GTAC 5 cut(s) 685, 792, 855, 928, 1157
AfiI CCNNNNNNNGG 6 cut(s) 220, 262, 901, 902, 1106, 1176
AgsI TTSAA 6 cut(s) 321, 511, 886, 1022, 1034, 1090
AjnI CCWGG 3 cut(s) 739, 942, 1161
AluBI AGCT 9 cut(s) 40, 58, 232, 314, 349, 386, 435, 452, 964
AluI AGCT 9 cut(s) 40, 58, 232, 314, 349, 386, 435, 452, 964
Alw21I GWGCWC 3 cut(s) 42, 351, 939
Alw26I GTCTC 4 cut(s) 72, 162, 164, 515
AoxI GGCC 2 cut(s) 367, 940
ApeKI GCWGC 3 cut(s) 383, 432, 452
ApoI RAATTY 1 cut(s) 673
ArsI GACNNNNNNTTYG 2 cut(s) 142, 174
Asp718I GGTACC 1 cut(s) 926
AspS9I GGNCC 1 cut(s) 737
AsuHPI GGTGA 1 cut(s) 599
AvaII GGWCC 1 cut(s) 737
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BanI GGYRCC 3 cut(s) 485, 926, 984
BanII GRGCYC 2 cut(s) 42, 351
BauI CACGAG 2 cut(s) 378, 623
Bbv12I GWGCWC 3 cut(s) 42, 351, 939
BbvI GCAGC 3 cut(s) 395, 439, 444
BccI CCATC 3 cut(s) 68, 618, 1178
BceAI ACGGC 1 cut(s) 955
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciT130I CCWGG 3 cut(s) 741, 944, 1163
BciVI GTATCC 1 cut(s) 839
BclI TGATCA 1 cut(s) 724
BcoDI GTCTC 4 cut(s) 72, 162, 164, 515
BfaI CTAG 1 cut(s) 518
BfuI GTATCC 1 cut(s) 839
BglI GCCNNNNNGGC 1 cut(s) 366
BisI GCNGC 3 cut(s) 384, 433, 453
BlsI GCNGC 3 cut(s) 385, 434, 454
BmcAI AGTACT 1 cut(s) 1157
Bme1390I CCNGG 3 cut(s) 741, 944, 1163
Bme18I GGWCC 1 cut(s) 737
BmgT120I GGNCC 1 cut(s) 737
BmiI GGNNCC 8 cut(s) 51, 225, 487, 669, 738, 928, 986, 1167
BmrFI CCNGG 3 cut(s) 741, 944, 1163
BmsI GCATC 2 cut(s) 589, 872
BplI GAGNNNNNCTC 2 cut(s) 529, 561
BpuEI CTTGAG 1 cut(s) 365
Bsa29I ATCGAT 1 cut(s) 72
BsaBI GATNNNNATC 2 cut(s) 862, 1041
BsaJI CCNNGG 3 cut(s) 762, 818, 930
BsaWI WCCGGW 1 cut(s) 142
Bsc4I CCNNNNNNNGG 6 cut(s) 220, 262, 901, 902, 1106, 1176
Bse118I RCCGGY 1 cut(s) 365
Bse3DI GCAATG 1 cut(s) 481
Bse8I GATNNNNATC 2 cut(s) 862, 1041
BseBI CCWGG 3 cut(s) 741, 944, 1163
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 3 cut(s) 762, 818, 930
BseGI GGATG 1 cut(s) 324
BseJI GATNNNNATC 2 cut(s) 862, 1041
BseLI CCNNNNNNNGG 6 cut(s) 220, 262, 901, 902, 1106, 1176
BseMI GCAATG 1 cut(s) 481
BseMII CTCAG 5 cut(s) 27, 450, 647, 669, 951
BseRI GAGGAG 5 cut(s) 194, 233, 236, 275, 594
BseXI GCAGC 3 cut(s) 395, 439, 444
BseYI CCCAGC 2 cut(s) 733, 1013
BsgI GTGCAG 1 cut(s) 402
BshFI GGCC 2 cut(s) 369, 942
BshNI GGYRCC 3 cut(s) 485, 926, 984
BshVI ATCGAT 1 cut(s) 72
BsiHKAI GWGCWC 3 cut(s) 42, 351, 939
BsiSI CCGG 2 cut(s) 143, 366
BslI CCNNNNNNNGG 6 cut(s) 220, 262, 901, 902, 1106, 1176
BsmAI GTCTC 4 cut(s) 72, 162, 164, 515
BsmI GAATGC 2 cut(s) 466, 896
BsnI GGCC 2 cut(s) 369, 942
Bsp1286I GDGCHC 3 cut(s) 42, 351, 939
Bsp143I GATC 3 cut(s) 408, 565, 724
Bsp19I CCATGG 1 cut(s) 762
BspANI GGCC 2 cut(s) 369, 942
BspCNI CTCAG 5 cut(s) 28, 449, 648, 670, 952
BspDI ATCGAT 1 cut(s) 72
BspLI GGNNCC 8 cut(s) 51, 225, 487, 669, 738, 928, 986, 1167
BspT107I GGYRCC 3 cut(s) 485, 926, 984
BsrDI GCAATG 1 cut(s) 481
BsrFI RCCGGY 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 3 cut(s) 762, 818, 930
BssMI GATC 3 cut(s) 408, 565, 724
BssNAI GTATAC 1 cut(s) 1084
BssSI CACGAG 2 cut(s) 378, 623
BssT1I CCWWGG 2 cut(s) 762, 818
Bst1107I GTATAC 1 cut(s) 1084
Bst2BI CACGAG 2 cut(s) 378, 623
Bst2UI CCWGG 3 cut(s) 741, 944, 1163
Bst4CI ACNGT 3 cut(s) 298, 641, 757
Bst6I CTCTTC 4 cut(s) 107, 191, 276, 554
BstC8I GCNNGC 2 cut(s) 351, 367
BstDEI CTNAG 7 cut(s) 36, 436, 569, 656, 678, 813, 960
BstDSI CCRYGG 1 cut(s) 762
BstF5I GGATG 1 cut(s) 324
BstKTI GATC 3 cut(s) 411, 568, 727
BstMAI GTCTC 4 cut(s) 72, 162, 164, 515
BstMBI GATC 3 cut(s) 408, 565, 724
BstMWI GCNNNNNNNGC 4 cut(s) 366, 383, 461, 1133
BstNI CCWGG 3 cut(s) 741, 944, 1163
BstSCI CCNGG 3 cut(s) 739, 942, 1161
BstV1I GCAGC 3 cut(s) 395, 439, 444
BstZ17I GTATAC 1 cut(s) 1084
Bsu15I ATCGAT 1 cut(s) 72
BsuI GTATCC 1 cut(s) 839
BsuRI GGCC 2 cut(s) 369, 942
BsuTUI ATCGAT 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 762
BtgZI GCGATG 1 cut(s) 1151
BtsCI GGATG 1 cut(s) 324
BtsI GCAGTG 1 cut(s) 1188
BtsIMutI CAGTG 3 cut(s) 294, 653, 1188
Cac8I GCNNGC 2 cut(s) 351, 367
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 737
ClaI ATCGAT 1 cut(s) 72
Csp6I GTAC 5 cut(s) 684, 791, 854, 927, 1156
CspCI CAANNNNNGTGG 4 cut(s) 463, 498, 516, 551
CviAII CATG 2 cut(s) 122, 763
CviQI GTAC 5 cut(s) 684, 791, 854, 927, 1156
DdeI CTNAG 7 cut(s) 36, 436, 569, 656, 678, 813, 960
DpnI GATC 3 cut(s) 410, 567, 726
DpnII GATC 3 cut(s) 408, 565, 724
DraIII CACNNNGTG 1 cut(s) 659
EaeI YGGCCR 1 cut(s) 367
Eam1104I CTCTTC 4 cut(s) 107, 191, 276, 554
EarI CTCTTC 4 cut(s) 107, 191, 276, 554
Ecl136II GAGCTC 2 cut(s) 40, 349
Eco130I CCWWGG 2 cut(s) 762, 818
Eco24I GRGCYC 2 cut(s) 42, 351
Eco47I GGWCC 1 cut(s) 737
Eco53kI GAGCTC 2 cut(s) 40, 349
Eco57I CTGAAG 1 cut(s) 1136
EcoICRI GAGCTC 2 cut(s) 40, 349
EcoRII CCWGG 3 cut(s) 739, 942, 1161
EcoT14I CCWWGG 2 cut(s) 762, 818
EcoT22I ATGCAT 1 cut(s) 865
EcoT38I GRGCYC 2 cut(s) 42, 351
ErhI CCWWGG 2 cut(s) 762, 818
FaeI CATG 2 cut(s) 125, 766
FatI CATG 2 cut(s) 121, 762
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 1 cut(s) 1083
Fnu4HI GCNGC 3 cut(s) 384, 433, 453
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 2 cut(s) 42, 351
Fsp4HI GCNGC 3 cut(s) 384, 433, 453
FspBI CTAG 1 cut(s) 518
GluI GCNGC 3 cut(s) 384, 433, 453
GsaI CCCAGC 2 cut(s) 737, 1017
HaeIII GGCC 2 cut(s) 369, 942
HapII CCGG 2 cut(s) 143, 366
Hin1II CATG 2 cut(s) 125, 766
HinfI GANTC 7 cut(s) 32, 80, 157, 170, 399, 446, 750
HpaII CCGG 2 cut(s) 143, 366
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 3 cut(s) 1007, 1084, 1100
Hpy188I TCNGA 7 cut(s) 37, 398, 439, 469, 679, 934, 961
Hpy188III TCNNGA 6 cut(s) 26, 161, 508, 518, 596, 1067
Hpy8I GTNNAC 3 cut(s) 1007, 1084, 1100
Hpy99I CGWCG 2 cut(s) 167, 407
HpyAV CCTTC 2 cut(s) 169, 638
HpyCH4III ACNGT 3 cut(s) 298, 641, 757
HpyCH4IV ACGT 1 cut(s) 1062
HpyCH4V TGCA 3 cut(s) 383, 863, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 366, 383, 461, 1133
HpyF3I CTNAG 7 cut(s) 36, 436, 569, 656, 678, 813, 960
HpySE526I ACGT 1 cut(s) 1062
Hsp92II CATG 2 cut(s) 125, 766
KpnI GGTACC 1 cut(s) 930
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 3 cut(s) 408, 565, 724
LmnI GCTCC 6 cut(s) 49, 145, 223, 607, 673, 934
Lsp1109I GCAGC 3 cut(s) 395, 439, 444
LweI GCATC 2 cut(s) 589, 872
MaeI CTAG 1 cut(s) 518
MaeII ACGT 1 cut(s) 1062
MaeIII GTNAC 4 cut(s) 235, 649, 751, 908
MalI GATC 3 cut(s) 410, 567, 726
MboI GATC 3 cut(s) 408, 565, 724
MhlI GDGCHC 3 cut(s) 42, 351, 939
MluCI AATT 7 cut(s) 44, 372, 390, 673, 744, 869, 948
MlyI GAGTC 5 cut(s) 41, 74, 164, 166, 759
Mph1103I ATGCAT 1 cut(s) 865
MroNI GCCGGC 1 cut(s) 365
MseI TTAA 4 cut(s) 704, 872, 1077, 1195
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 2 cut(s) 143, 366
MspR9I CCNGG 3 cut(s) 741, 944, 1163
Mva1269I GAATGC 2 cut(s) 466, 896
MvaI CCWGG 3 cut(s) 741, 944, 1163
MwoI GCNNNNNNNGC 4 cut(s) 366, 383, 461, 1133
NaeI GCCGGC 1 cut(s) 367
NcoI CCATGG 1 cut(s) 762
NdeII GATC 3 cut(s) 408, 565, 724
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 2 cut(s) 125, 766
NlaIV GGNNCC 8 cut(s) 51, 225, 487, 669, 738, 928, 986, 1167
NmuCI GTSAC 3 cut(s) 235, 649, 751
NsiI ATGCAT 1 cut(s) 865
PcsI WCGNNNNNNNCGW 3 cut(s) 245, 408, 417
PctI GAATGC 2 cut(s) 466, 896
PdiI GCCGGC 1 cut(s) 367
PfeI GAWTC 2 cut(s) 399, 446
PfoI TCCNGGA 2 cut(s) 739, 1161
PkrI GCNGC 3 cut(s) 385, 434, 454
PleI GAGTC 5 cut(s) 40, 74, 164, 165, 758
PpsI GAGTC 5 cut(s) 40, 74, 164, 165, 758
Psp124BI GAGCTC 2 cut(s) 42, 351
Psp1406I AACGTT 1 cut(s) 1062
Psp6I CCWGG 3 cut(s) 739, 942, 1161
PspFI CCCAGC 2 cut(s) 733, 1013
PspGI CCWGG 3 cut(s) 739, 942, 1161
PspN4I GGNNCC 8 cut(s) 51, 225, 487, 669, 738, 928, 986, 1167
PspPI GGNCC 1 cut(s) 737
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 5 cut(s) 685, 792, 855, 928, 1157
RsaNI GTAC 5 cut(s) 684, 791, 854, 927, 1156
SacI GAGCTC 2 cut(s) 42, 351
SaqAI TTAA 4 cut(s) 704, 872, 1077, 1195
SatI GCNGC 3 cut(s) 384, 433, 453
Sau3AI GATC 3 cut(s) 408, 565, 724
Sau96I GGNCC 1 cut(s) 737
ScaI AGTACT 1 cut(s) 1157
SchI GAGTC 5 cut(s) 41, 74, 164, 166, 759
ScrFI CCNGG 3 cut(s) 741, 944, 1163
SduI GDGCHC 3 cut(s) 42, 351, 939
SfaNI GCATC 2 cut(s) 589, 872
SinI GGWCC 1 cut(s) 737
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 7 cut(s) 44, 372, 390, 673, 744, 869, 948
SspI AATATT 1 cut(s) 533
SspMI CTAG 1 cut(s) 518
SstI GAGCTC 2 cut(s) 42, 351
StyD4I CCNGG 3 cut(s) 739, 942, 1161
StyI CCWWGG 2 cut(s) 762, 818
TaaI ACNGT 3 cut(s) 298, 641, 757
TaiI ACGT 1 cut(s) 1065
TasI AATT 7 cut(s) 44, 372, 390, 673, 744, 869, 948
TatI WGTACW 1 cut(s) 1155
TfiI GAWTC 2 cut(s) 399, 446
Tru1I TTAA 4 cut(s) 704, 872, 1077, 1195
Tru9I TTAA 4 cut(s) 704, 872, 1077, 1195
TscAI CASTG 3 cut(s) 301, 660, 1188
TseFI GTSAC 3 cut(s) 235, 649, 751
TseI GCWGC 3 cut(s) 383, 432, 452
Tsp45I GTSAC 3 cut(s) 235, 649, 751
TspDTI ATGAA 3 cut(s) 138, 492, 945
TspGWI ACGGA 3 cut(s) 146, 180, 254
TspRI CASTG 3 cut(s) 301, 660, 1188
VpaK11BI GGWCC 1 cut(s) 737
XapI RAATTY 1 cut(s) 673
XbaI TCTAGA 1 cut(s) 517
XmiI GTMKAC 1 cut(s) 1083
XspI CTAG 1 cut(s) 518
ZrmI AGTACT 1 cut(s) 1157
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.