RLG00000007929

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003646
Physical Location & Seq
Forward (+)
23090971 .. 23094193
3223 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007929

Sequence Viewer

Length: 1038 bp
ATGGGTAAAAAGAAGAAGAGAACCGAAGCGGAAGAAGAAATCAAAGCCGACAACATTGTTAACGGCGACTCGCATAACAAAACCAAGAAGAACAAGAACAAGAAACCCAAAAACGACACCGCCGCCGTGACTCCTACCGTCACCGTAGCCATCGCCGGTTCCATCATTCACAACGCTCAATCCCTCGAACTCGCCACTCGGTTGGCCGGTCAGATAGCTCGTGCCGTCACGATTTTCCGAGTCGATGAGGTGGTGGTATTTGACAACAATGGTGAGTCTGGCTCGCCGGAAGCTAATAATTCCGATGAGAATGAAAGCGGGGCTGCTTTTCTTATAAGAATCTTGCGGTATCTTGAGACGCCGCAGTATCTGAGGAAAGCTCTATTTCCGAAACATAATAGCTTGAGATTTGTGGGTCTGTTGCCTCCGCTTGATGCGCCGCACCATCTGCGTAAACACGAATGGGGCGCATTTCGGGAAGGTGTCACGCTAAAGGAAAGATCTCCGGATGGTGTTGGGACATTAGTTGATGTGGGCTTGAGTAAGAACGTTGTCATTGACCAAGTACTTGATCCTGGAACAAGAGTTACTGTGGCTATGGGAAACAGCTGGAATTTGGATGCTGATATATCACGGCAGGTTGTATCATCATCCAAGCCAAGGGAAGAAGGAGGGATGTATTGGGGGTACAAAGTGCGGTATGCTTCCAATATCACTTCGGTAATGACTGAATGTCCGTACGAGGGTGGTTATGATCATTTGATTGGAACCTCAGAGCATGGTCAGATTATAAATTCCTCTGATCTCAATATACCTACTTTCAGGCATCTATTGATTGCTTTCGGTGGACTTGCTGGATTGGAAGAGAGCATTGAAGAGGATCATAACTTAAAGGCAAAGAATGTGCGGGAGGTGTTTAATATATATTTAAACACATGTCCGCAGCAGGGGAGTCGAACAATACGAACAGAGGAAGCAATTTTAATTTCACTTCAATATTTCCAAGAACCAATCAACAGAGCACTGCAGAGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

346

Amino Acids

38.26

Weight (kDa)

6.81

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 46 - 333 1.4e-109 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 335, 791
Acc36I ACCTGC 1 cut(s) 628
AccIII TCCGGA 1 cut(s) 505
AclI AACGTT 1 cut(s) 549
AclWI GGATC 2 cut(s) 566, 888
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 2 cut(s) 613, 793
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 3 cut(s) 567, 689, 740
AfiI CCNNNNNNNGG 3 cut(s) 660, 743, 947
AflIII ACRYGT 1 cut(s) 935
AgsI TTSAA 2 cut(s) 875, 995
AhdI GACNNNNNGTC 1 cut(s) 732
AjnI CCWGG 1 cut(s) 574
AluBI AGCT 5 cut(s) 218, 293, 380, 402, 609
AluI AGCT 5 cut(s) 218, 293, 380, 402, 609
Alw21I GWGCWC 1 cut(s) 1024
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 2 cut(s) 566, 888
AlwNI CAGNNNCTG 1 cut(s) 370
Aor13HI TCCGGA 1 cut(s) 505
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 2 cut(s) 323, 943
ApoI RAATTY 2 cut(s) 613, 793
AspLEI GCGC 2 cut(s) 439, 470
AsuHPI GGTGA 2 cut(s) 133, 284
BauI CACGAG 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 1024
BbvI GCAGC 2 cut(s) 310, 955
BccI CCATC 4 cut(s) 158, 170, 453, 503
BceAI ACGGC 4 cut(s) 79, 110, 209, 650
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 1 cut(s) 576
BclI TGATCA 1 cut(s) 754
BcoDI GTCTC 1 cut(s) 350
BfmI CTRYAG 1 cut(s) 1025
BfuAI ACCTGC 1 cut(s) 628
BglII AGATCT 1 cut(s) 500
BisI GCNGC 5 cut(s) 123, 324, 362, 440, 944
BlsI GCNGC 5 cut(s) 124, 325, 363, 441, 945
BmcAI AGTACT 1 cut(s) 567
Bme1390I CCNGG 1 cut(s) 576
BmeRI GACNNNNNGTC 1 cut(s) 732
BmiI GGNNCC 2 cut(s) 160, 769
BmrFI CCNGG 1 cut(s) 576
BmsI GCATC 3 cut(s) 424, 610, 835
BplI GAGNNNNNCTC 4 cut(s) 266, 298, 364, 396
BpuEI CTTGAG 3 cut(s) 374, 424, 559
BsaHI GRCGYC 1 cut(s) 359
BsaJI CCNNGG 1 cut(s) 659
BsaWI WCCGGW 1 cut(s) 505
Bsc4I CCNNNNNNNGG 3 cut(s) 660, 743, 947
Bse118I RCCGGY 2 cut(s) 155, 206
BseAI TCCGGA 1 cut(s) 505
BseBI CCWGG 1 cut(s) 576
BseDI CCNNGG 1 cut(s) 659
BseGI GGATG 4 cut(s) 514, 625, 650, 681
BseLI CCNNNNNNNGG 3 cut(s) 660, 743, 947
BseMII CTCAG 2 cut(s) 362, 786
BseXI GCAGC 2 cut(s) 310, 955
BshFI GGCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 1024
BsiSI CCGG 4 cut(s) 156, 207, 287, 506
BsiWI CGTACG 1 cut(s) 738
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 3 cut(s) 660, 743, 947
BsmAI GTCTC 1 cut(s) 350
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 1024
Bsp13I TCCGGA 1 cut(s) 505
Bsp143I GATC 5 cut(s) 500, 571, 754, 802, 880
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 2 cut(s) 363, 785
BspEI TCCGGA 1 cut(s) 505
BspLI GGNNCC 2 cut(s) 160, 769
BspMAI CTGCAG 1 cut(s) 1029
BspMI ACCTGC 1 cut(s) 628
BspPI GGATC 2 cut(s) 566, 888
BsrFI RCCGGY 2 cut(s) 155, 206
BssAI RCCGGY 2 cut(s) 155, 206
BssECI CCNNGG 1 cut(s) 659
BssMI GATC 5 cut(s) 500, 571, 754, 802, 880
BssNI GRCGYC 1 cut(s) 359
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 659
Bst2BI CACGAG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 3 cut(s) 139, 145, 592
Bst6I CTCTTC 3 cut(s) 11, 858, 870
BstACI GRCGYC 1 cut(s) 359
BstAPI GCANNNNNTGC 1 cut(s) 448
BstC8I GCNNGC 1 cut(s) 284
BstDEI CTNAG 2 cut(s) 371, 772
BstF5I GGATG 4 cut(s) 514, 625, 650, 681
BstHHI GCGC 2 cut(s) 439, 470
BstKTI GATC 5 cut(s) 503, 574, 757, 805, 883
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 5 cut(s) 500, 571, 754, 802, 880
BstMWI GCNNNNNNNGC 2 cut(s) 436, 448
BstNI CCWGG 1 cut(s) 576
BstNSI RCATGY 1 cut(s) 939
BstSCI CCNGG 1 cut(s) 574
BstSFI CTRYAG 1 cut(s) 1025
BstV1I GCAGC 2 cut(s) 310, 955
BstX2I RGATCY 1 cut(s) 500
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 500
BsuRI GGCC 1 cut(s) 206
BtgZI GCGATG 1 cut(s) 136
BtsCI GGATG 4 cut(s) 514, 625, 650, 681
BtsI GCAGTG 1 cut(s) 1022
BtsIMutI CAGTG 1 cut(s) 1022
BveI ACCTGC 1 cut(s) 628
Cac8I GCNNGC 1 cut(s) 284
CaiI CAGNNNCTG 1 cut(s) 370
CfoI GCGC 2 cut(s) 439, 470
Cfr10I RCCGGY 2 cut(s) 155, 206
CseI GACGC 1 cut(s) 367
Csp6I GTAC 3 cut(s) 566, 688, 739
CviAII CATG 2 cut(s) 779, 936
CviQI GTAC 3 cut(s) 566, 688, 739
DdeI CTNAG 2 cut(s) 371, 772
DpnI GATC 5 cut(s) 502, 573, 756, 804, 882
DpnII GATC 5 cut(s) 500, 571, 754, 802, 880
DraI TTTAAA 1 cut(s) 930
DriI GACNNNNNGTC 1 cut(s) 732
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 3 cut(s) 11, 858, 870
Eam1105I GACNNNNNGTC 1 cut(s) 732
EarI CTCTTC 3 cut(s) 11, 858, 870
Eco130I CCWWGG 1 cut(s) 659
EcoRII CCWGG 1 cut(s) 574
EcoT14I CCWWGG 1 cut(s) 659
ErhI CCWWGG 1 cut(s) 659
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 2 cut(s) 782, 939
FaqI GGGAC 1 cut(s) 532
FatI CATG 2 cut(s) 778, 935
FauI CCCGC 2 cut(s) 311, 900
FbaI TGATCA 1 cut(s) 754
Fnu4HI GCNGC 5 cut(s) 123, 324, 362, 440, 944
FokI GGATG 4 cut(s) 521, 632, 637, 688
Fsp4HI GCNGC 5 cut(s) 123, 324, 362, 440, 944
GlaI GCGC 2 cut(s) 438, 469
GluI GCNGC 5 cut(s) 123, 324, 362, 440, 944
HaeIII GGCC 1 cut(s) 206
HapII CCGG 4 cut(s) 156, 207, 287, 506
HgaI GACGC 1 cut(s) 367
HhaI GCGC 2 cut(s) 439, 470
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 2 cut(s) 782, 939
Hin6I GCGC 2 cut(s) 437, 468
HinP1I GCGC 2 cut(s) 437, 468
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 6 cut(s) 68, 130, 240, 275, 339, 952
HpaI GTTAAC 1 cut(s) 61
HpaII CCGG 4 cut(s) 156, 207, 287, 506
HphI GGTGA 2 cut(s) 133, 284
Hpy166II GTNNAC 3 cut(s) 61, 455, 848
Hpy188I TCNGA 8 cut(s) 213, 239, 304, 372, 390, 775, 786, 802
Hpy188III TCNNGA 4 cut(s) 229, 353, 476, 506
Hpy8I GTNNAC 3 cut(s) 61, 455, 848
HpyAV CCTTC 2 cut(s) 473, 662
HpyCH4III ACNGT 3 cut(s) 139, 145, 592
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 1 cut(s) 1027
HpyF10VI GCNNNNNNNGC 2 cut(s) 436, 448
HpyF3I CTNAG 2 cut(s) 371, 772
HpySE526I ACGT 1 cut(s) 549
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 2 cut(s) 782, 939
HspAI GCGC 2 cut(s) 437, 468
Kpn2I TCCGGA 1 cut(s) 505
Ksp22I TGATCA 1 cut(s) 754
KspAI GTTAAC 1 cut(s) 61
Kzo9I GATC 5 cut(s) 500, 571, 754, 802, 880
Lsp1109I GCAGC 2 cut(s) 310, 955
LweI GCATC 3 cut(s) 424, 610, 835
MaeII ACGT 1 cut(s) 549
MaeIII GTNAC 5 cut(s) 127, 139, 226, 484, 586
MalI GATC 5 cut(s) 502, 573, 756, 804, 882
MboI GATC 5 cut(s) 500, 571, 754, 802, 880
MboII GAAGA 8 cut(s) 25, 28, 44, 47, 100, 677, 875, 887
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 1024
MluCI AATT 5 cut(s) 298, 613, 793, 978, 984
MlyI GAGTC 5 cut(s) 62, 124, 249, 284, 961
MroI TCCGGA 1 cut(s) 505
MseI TTAA 5 cut(s) 60, 890, 918, 929, 983
MspA1I CMGCKG 1 cut(s) 609
MspI CCGG 4 cut(s) 156, 207, 287, 506
MspR9I CCNGG 1 cut(s) 576
MvaI CCWGG 1 cut(s) 576
MwoI GCNNNNNNNGC 2 cut(s) 436, 448
NdeII GATC 5 cut(s) 500, 571, 754, 802, 880
NlaIII CATG 2 cut(s) 782, 939
NlaIV GGNNCC 2 cut(s) 160, 769
NmuCI GTSAC 4 cut(s) 127, 139, 226, 484
NspI RCATGY 1 cut(s) 939
PciI ACATGT 1 cut(s) 935
PcsI WCGNNNNNNNCGW 1 cut(s) 961
PfeI GAWTC 1 cut(s) 339
Pfl23II CGTACG 1 cut(s) 738
PfoI TCCNGGA 1 cut(s) 574
PkrI GCNGC 5 cut(s) 124, 325, 363, 441, 945
PleI GAGTC 5 cut(s) 62, 124, 248, 283, 960
PpsI GAGTC 5 cut(s) 62, 124, 248, 283, 960
PscI ACATGT 1 cut(s) 935
PsiI TTATAA 2 cut(s) 335, 791
Psp1406I AACGTT 1 cut(s) 549
Psp6I CCWGG 1 cut(s) 574
PspGI CCWGG 1 cut(s) 574
PspLI CGTACG 1 cut(s) 738
PspN4I GGNNCC 2 cut(s) 160, 769
PsrI GAACNNNNNNTAC 2 cut(s) 571, 603
PstI CTGCAG 1 cut(s) 1029
PstNI CAGNNNCTG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 500
PvuII CAGCTG 1 cut(s) 609
RsaI GTAC 3 cut(s) 567, 689, 740
RsaNI GTAC 3 cut(s) 566, 688, 739
SaqAI TTAA 5 cut(s) 60, 890, 918, 929, 983
SatI GCNGC 5 cut(s) 123, 324, 362, 440, 944
Sau3AI GATC 5 cut(s) 500, 571, 754, 802, 880
ScaI AGTACT 1 cut(s) 567
SchI GAGTC 5 cut(s) 62, 124, 249, 284, 961
ScrFI CCNGG 1 cut(s) 576
SduI GDGCHC 1 cut(s) 1024
SfaNI GCATC 3 cut(s) 424, 610, 835
SfcI CTRYAG 1 cut(s) 1025
SmlI CTYRAG 3 cut(s) 353, 403, 538
SmoI CTYRAG 3 cut(s) 353, 403, 538
Sse9I AATT 5 cut(s) 298, 613, 793, 978, 984
SspI AATATT 1 cut(s) 998
StyD4I CCNGG 1 cut(s) 574
StyI CCWWGG 1 cut(s) 659
TaaI ACNGT 3 cut(s) 139, 145, 592
TaiI ACGT 1 cut(s) 552
TaqI TCGA 3 cut(s) 186, 243, 955
TasI AATT 5 cut(s) 298, 613, 793, 978, 984
TatI WGTACW 1 cut(s) 565
TauI GCSGC 3 cut(s) 125, 364, 442
TfiI GAWTC 1 cut(s) 339
Tru1I TTAA 5 cut(s) 60, 890, 918, 929, 983
Tru9I TTAA 5 cut(s) 60, 890, 918, 929, 983
TscAI CASTG 1 cut(s) 1029
TseFI GTSAC 4 cut(s) 127, 139, 226, 484
TseI GCWGC 2 cut(s) 323, 943
Tsp45I GTSAC 4 cut(s) 127, 139, 226, 484
TspDTI ATGAA 1 cut(s) 327
TspGWI ACGGA 1 cut(s) 726
TspRI CASTG 1 cut(s) 1029
XapI RAATTY 2 cut(s) 613, 793
XceI RCATGY 1 cut(s) 939
ZrmI AGTACT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.