Rroxscaffold_5G00348510

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003646
Physical Location & Seq
Forward (+)
20769089 .. 20775005
5917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348510.1

Sequence Viewer

Length: 525 bp
ATGAAAACAAGAACAAAAGCCCACCCACCAATACGACGGTACTACCATCTTTGCGGCTCACGCCGAAATCGATTTGCAAGCGAACTTGTCGGCGGTGACCTTGTCGATACGGAGGTAGGTGATTCGAATTCTTCGCAGCCGAGTTCATATGTCTGCTCGATTTCGGTGTCGGTCAGGTCTTCAAGACAGAGAGCTTGCTGCCGCCATGGTGACGGCGTAGTCTATGGCAAATGGGTTCGGCTGTTCTTCTTGTGCTTTAAGAGCGAACCCGTTTTTGGGGAGGATGCATCAAGGTTAACAGTAGGCATAATTCATGTTGAATATTTTGGTTTAACAATTGATTGCTCTGAGCAACTATTGATTGCATTTGGTGGACTTGCTGGGTTGGAAGAGAGCATTGAGGAGGATCATAACTTAAAGGTATGGTTGCATAGGGATTTGGTTTGGGACTCGAATGTTAGACGCTGGGAAGGTGCAAGCCTTTGCTCTGTAATGCTAGAAATCAGCTACAAGCTCTATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

174

Amino Acids

19.76

Weight (kDa)

6.59

Isoelectric Point (pI)

57.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AciI CCGC 3 cut(s) 54, 93, 202
AclWI GGATC 1 cut(s) 414
AcsI RAATTY 1 cut(s) 127
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 275, 276
AgsI TTSAA 2 cut(s) 183, 320
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AluBI AGCT 3 cut(s) 194, 507, 514
AluI AGCT 3 cut(s) 194, 507, 514
AlwI GGATC 1 cut(s) 414
ApeKI GCWGC 2 cut(s) 136, 198
ApoI RAATTY 1 cut(s) 127
AsuHPI GGTGA 3 cut(s) 107, 131, 221
AsuII TTCGAA 1 cut(s) 125
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BbsI GAAGAC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 148, 185
BccI CCATC 1 cut(s) 54
BceAI ACGGC 1 cut(s) 229
BfaI CTAG 1 cut(s) 497
BisI GCNGC 4 cut(s) 55, 137, 199, 202
BlsI GCNGC 4 cut(s) 56, 138, 200, 203
BmsI GCATC 2 cut(s) 274, 296
BpiI GAAGAC 1 cut(s) 171
Bpu14I TTCGAA 1 cut(s) 125
Bsa29I ATCGAT 1 cut(s) 70
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 276
BseCI ATCGAT 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 2 cut(s) 275, 276
BseMII CTCAG 1 cut(s) 339
BseRI GAGGAG 1 cut(s) 416
BseXI GCAGC 2 cut(s) 148, 185
BseYI CCCAGC 2 cut(s) 380, 465
BshVI ATCGAT 1 cut(s) 70
BslFI GGGAC 1 cut(s) 461
BslI CCNNNNNNNGG 2 cut(s) 275, 276
BsmFI GGGAC 1 cut(s) 461
Bsp119I TTCGAA 1 cut(s) 125
Bsp143I GATC 1 cut(s) 406
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 3 cut(s) 54, 93, 202
BspCNI CTCAG 1 cut(s) 340
BspDI ATCGAT 1 cut(s) 70
BspPI GGATC 1 cut(s) 414
BspT104I TTCGAA 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 1 cut(s) 406
BssT1I CCWWGG 1 cut(s) 205
Bst4CI ACNGT 2 cut(s) 39, 301
Bst6I CTCTTC 1 cut(s) 384
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 3 cut(s) 79, 196, 478
BstDEI CTNAG 1 cut(s) 348
BstDSI CCRYGG 1 cut(s) 205
BstEII GGTNACC 1 cut(s) 95
BstF5I GGATG 1 cut(s) 289
BstKTI GATC 1 cut(s) 409
BstMBI GATC 1 cut(s) 406
BstMWI GCNNNNNNNGC 2 cut(s) 60, 261
BstPI GGTNACC 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 148, 185
BstV2I GAAGAC 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 70
BsuTUI ATCGAT 1 cut(s) 70
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 1 cut(s) 289
Cac8I GCNNGC 3 cut(s) 79, 196, 478
ClaI ATCGAT 1 cut(s) 70
CseI GACGC 1 cut(s) 471
Csp6I GTAC 1 cut(s) 40
CviAII CATG 2 cut(s) 206, 314
CviJI RGCY 8 cut(s) 20, 57, 139, 194, 241, 480, 507, 514
CviKI_1 RGCY 8 cut(s) 20, 57, 139, 194, 241, 480, 507, 514
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 1 cut(s) 348
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 205
Eco91I GGTNACC 1 cut(s) 95
EcoO65I GGTNACC 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 2 cut(s) 209, 317
FaqI GGGAC 1 cut(s) 461
FatI CATG 2 cut(s) 205, 313
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 4 cut(s) 55, 137, 199, 202
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 4 cut(s) 55, 137, 199, 202
FspBI CTAG 1 cut(s) 497
GluI GCNGC 4 cut(s) 55, 137, 199, 202
GsaI CCCAGC 2 cut(s) 384, 469
HgaI GACGC 1 cut(s) 471
Hin1II CATG 2 cut(s) 209, 317
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HinfI GANTC 2 cut(s) 122, 449
HpaI GTTAAC 1 cut(s) 297
HphI GGTGA 3 cut(s) 107, 131, 221
Hpy166II GTNNAC 2 cut(s) 297, 374
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 1 cut(s) 183
Hpy8I GTNNAC 2 cut(s) 297, 374
Hpy99I CGWCG 1 cut(s) 39
HpyAV CCTTC 1 cut(s) 464
HpyCH4III ACNGT 2 cut(s) 39, 301
HpyCH4V TGCA 5 cut(s) 77, 287, 365, 430, 476
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 261
HpyF3I CTNAG 1 cut(s) 348
Hsp92II CATG 2 cut(s) 209, 317
KspAI GTTAAC 1 cut(s) 297
Kzo9I GATC 1 cut(s) 406
LpnPI CCDG 3 cut(s) 160, 366, 451
Lsp1109I GCAGC 2 cut(s) 148, 185
LweI GCATC 2 cut(s) 274, 296
MaeI CTAG 1 cut(s) 497
MaeIII GTNAC 2 cut(s) 95, 209
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MboII GAAGA 4 cut(s) 123, 171, 238, 401
MfeI CAATTG 1 cut(s) 336
MluCI AATT 3 cut(s) 127, 309, 336
MlyI GAGTC 1 cut(s) 443
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 4 cut(s) 106, 274, 394, 397
Mph1103I ATGCAT 1 cut(s) 289
MseI TTAA 4 cut(s) 258, 296, 332, 416
MunI CAATTG 1 cut(s) 336
MwoI GCNNNNNNNGC 2 cut(s) 60, 261
NcoI CCATGG 1 cut(s) 205
NdeI CATATG 1 cut(s) 148
NdeII GATC 1 cut(s) 406
NlaIII CATG 2 cut(s) 209, 317
NmeAIII GCCGAG 1 cut(s) 165
NmuCI GTSAC 2 cut(s) 95, 209
NsiI ATGCAT 1 cut(s) 289
NspV TTCGAA 1 cut(s) 125
PcsI WCGNNNNNNNCGW 1 cut(s) 67
PfeI GAWTC 1 cut(s) 122
PflFI GACNNNGTC 1 cut(s) 101
PkrI GCNGC 4 cut(s) 56, 138, 200, 203
PleI GAGTC 1 cut(s) 443
PpsI GAGTC 1 cut(s) 443
PspEI GGTNACC 1 cut(s) 95
PspFI CCCAGC 2 cut(s) 380, 465
PsyI GACNNNGTC 1 cut(s) 101
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SaqAI TTAA 4 cut(s) 258, 296, 332, 416
SatI GCNGC 4 cut(s) 55, 137, 199, 202
Sau3AI GATC 1 cut(s) 406
SchI GAGTC 1 cut(s) 443
SfaNI GCATC 2 cut(s) 274, 296
SfuI TTCGAA 1 cut(s) 125
Sse9I AATT 3 cut(s) 127, 309, 336
SsiI CCGC 3 cut(s) 54, 93, 202
SspI AATATT 1 cut(s) 323
SspMI CTAG 1 cut(s) 497
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 39, 301
TaqI TCGA 5 cut(s) 70, 105, 125, 158, 452
TaqII GACCGA 1 cut(s) 160
TasI AATT 3 cut(s) 127, 309, 336
TauI GCSGC 2 cut(s) 57, 204
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 4 cut(s) 258, 296, 332, 416
Tru9I TTAA 4 cut(s) 258, 296, 332, 416
TseFI GTSAC 2 cut(s) 95, 209
TseI GCWGC 2 cut(s) 136, 198
Tsp45I GTSAC 2 cut(s) 95, 209
TspDTI ATGAA 3 cut(s) 17, 135, 302
TspGWI ACGGA 1 cut(s) 125
Tth111I GACNNNGTC 1 cut(s) 101
XapI RAATTY 1 cut(s) 127
XspI CTAG 1 cut(s) 497
Zsp2I ATGCAT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.