Rroxscaffold_5G00360910

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003646
Physical Location & Seq
Reverse (-)
41563508 .. 41567370
3863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00360910.1

Sequence Viewer

Length: 1038 bp
ATGGGTAAAAAGAAGAAGAGAACCGAAGCGGAAGAAGAAATCAAAGCCGACGGCATTGTTAACGGTGACTCGCATAACAAAACCAAGAAGAACAAGAACAAGAAACCCAAAAACGACACCGCCGCCGTGACTCCTACCGTCACCGTAGCCATCGCCGGTTCCATCATTCACAACGCTCAATCCCTGGAACTCGCCACTCGGCTGGCCGGTCAGATAGCTCGTGCCGTCACGATTTTCCGAGTCGATGAGGTGGTGGTATTTGACAATAACGGTGAGTCTGGCTCGCCGGAAGCTAATAATTCCGATGAGAATGAAAGCGGGGCTGCTTTTCTTATTAGAATCTTGCGGTATCTTGAGACGCCGCAGTATCTGAGAAAAGCTCTATTTCCGAAACATAATAGCTTAAGATTTGTGGGTTTGTTGCCTCCCCTTGATGCGCCGCACCATCTGCGTAAACACGAATGGGGTGCATTTCGGGAAGGTGTCACGCTGAAGGAAAGATCTCCGGATGGTGTTGGGACATTAGTTGATGTGGGCTTGAGTAAGAATGTTGTTGTTGACCAAGTACTTGATCCTGGAACAAGAGTTACTGTGGCTATGGGAGACAGCTGGAATTTGGATGCTGATATATCACGGCAGGTTGTATCATCATCCAAGCCAAGGGAAGAAGCAGGGATGTATTGGGGGTACAAAGTGCGGTATGCTTCCAATATCACTTCGGTAATGACTGAATGTGCGTACGAGGGTGGTTATGATCATTTGATTGGAACCTCAGAGCATGGTCAGATTATAAATTCCTCTGATCTCAATATACCTACTTTCAGGCATCTATTGATTGCTTTTGGTGGACTTGCTGGATTGGAAGAGAGCATTGAAGAGGATCATAACTTAAAGGCAAAGAATGTGCGGGAGGTGTTTAATATATATTTAAACACATGTCCGCATCAGGGGAGTCGAACAATACGAACAGAGGAAGCAATTTTAATTTCACTTCAATATTTCCAAGAACCAATCAACAGAGCACTGCAGAGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

345

Amino Acids

38.18

Weight (kDa)

6.61

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 46 - 333 2.3e-109 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 791
Acc36I ACCTGC 1 cut(s) 628
AccIII TCCGGA 1 cut(s) 505
AclWI GGATC 2 cut(s) 566, 888
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 2 cut(s) 613, 793
AcuI CTGAAG 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 3 cut(s) 567, 689, 740
AfiI CCNNNNNNNGG 2 cut(s) 660, 947
AflII CTTAAG 1 cut(s) 403
AflIII ACRYGT 1 cut(s) 935
AgsI TTSAA 2 cut(s) 875, 995
AjnI CCWGG 2 cut(s) 183, 574
AluBI AGCT 5 cut(s) 218, 293, 380, 402, 609
AluI AGCT 5 cut(s) 218, 293, 380, 402, 609
Alw21I GWGCWC 1 cut(s) 1024
Alw26I GTCTC 2 cut(s) 350, 597
AlwI GGATC 2 cut(s) 566, 888
AlwNI CAGNNNCTG 1 cut(s) 370
Aor13HI TCCGGA 1 cut(s) 505
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 323
ApoI RAATTY 2 cut(s) 613, 793
AspLEI GCGC 1 cut(s) 439
AsuHPI GGTGA 3 cut(s) 77, 133, 284
BauI CACGAG 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 1024
BbvI GCAGC 1 cut(s) 310
BccI CCATC 4 cut(s) 158, 170, 453, 503
BceAI ACGGC 4 cut(s) 67, 110, 209, 650
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 2 cut(s) 185, 576
BclI TGATCA 1 cut(s) 754
BcoDI GTCTC 2 cut(s) 350, 597
BfmI CTRYAG 1 cut(s) 1025
BfrI CTTAAG 1 cut(s) 403
BfuAI ACCTGC 1 cut(s) 628
BglII AGATCT 1 cut(s) 500
BisI GCNGC 4 cut(s) 123, 324, 362, 440
BlsI GCNGC 4 cut(s) 124, 325, 363, 441
BmcAI AGTACT 1 cut(s) 567
Bme1390I CCNGG 2 cut(s) 185, 576
BmiI GGNNCC 2 cut(s) 160, 769
BmrFI CCNGG 2 cut(s) 185, 576
BmsI GCATC 4 cut(s) 424, 610, 835, 952
BplI GAGNNNNNCTC 4 cut(s) 266, 298, 364, 396
BpuEI CTTGAG 2 cut(s) 374, 559
BsaHI GRCGYC 1 cut(s) 359
BsaJI CCNNGG 2 cut(s) 183, 659
BsaWI WCCGGW 1 cut(s) 505
Bsc4I CCNNNNNNNGG 2 cut(s) 660, 947
Bse118I RCCGGY 2 cut(s) 155, 206
BseAI TCCGGA 1 cut(s) 505
BseBI CCWGG 2 cut(s) 185, 576
BseDI CCNNGG 2 cut(s) 183, 659
BseGI GGATG 4 cut(s) 514, 625, 650, 681
BseLI CCNNNNNNNGG 2 cut(s) 660, 947
BseMII CTCAG 2 cut(s) 362, 786
BseXI GCAGC 1 cut(s) 310
BshFI GGCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 1024
BsiSI CCGG 4 cut(s) 156, 207, 287, 506
BsiWI CGTACG 1 cut(s) 738
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 2 cut(s) 660, 947
BsmAI GTCTC 2 cut(s) 350, 597
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 1024
Bsp13I TCCGGA 1 cut(s) 505
Bsp143I GATC 5 cut(s) 500, 571, 754, 802, 880
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 2 cut(s) 363, 785
BspEI TCCGGA 1 cut(s) 505
BspLI GGNNCC 2 cut(s) 160, 769
BspMAI CTGCAG 1 cut(s) 1029
BspMI ACCTGC 1 cut(s) 628
BspPI GGATC 2 cut(s) 566, 888
BspTI CTTAAG 1 cut(s) 403
BsrFI RCCGGY 2 cut(s) 155, 206
BssAI RCCGGY 2 cut(s) 155, 206
BssECI CCNNGG 2 cut(s) 183, 659
BssMI GATC 5 cut(s) 500, 571, 754, 802, 880
BssNI GRCGYC 1 cut(s) 359
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 659
Bst2BI CACGAG 1 cut(s) 219
Bst2UI CCWGG 2 cut(s) 185, 576
Bst4CI ACNGT 5 cut(s) 65, 139, 145, 272, 592
Bst6I CTCTTC 3 cut(s) 11, 858, 870
BstACI GRCGYC 1 cut(s) 359
BstAFI CTTAAG 1 cut(s) 403
BstAPI GCANNNNNTGC 1 cut(s) 448
BstC8I GCNNGC 2 cut(s) 204, 284
BstDEI CTNAG 2 cut(s) 371, 772
BstF5I GGATG 4 cut(s) 514, 625, 650, 681
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 5 cut(s) 503, 574, 757, 805, 883
BstMAI GTCTC 2 cut(s) 350, 597
BstMBI GATC 5 cut(s) 500, 571, 754, 802, 880
BstMWI GCNNNNNNNGC 1 cut(s) 448
BstNI CCWGG 2 cut(s) 185, 576
BstNSI RCATGY 1 cut(s) 939
BstSCI CCNGG 2 cut(s) 183, 574
BstSFI CTRYAG 1 cut(s) 1025
BstV1I GCAGC 1 cut(s) 310
BstX2I RGATCY 1 cut(s) 500
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 500
BsuRI GGCC 1 cut(s) 206
BtgZI GCGATG 1 cut(s) 136
BtsCI GGATG 4 cut(s) 514, 625, 650, 681
BtsI GCAGTG 1 cut(s) 1022
BtsIMutI CAGTG 1 cut(s) 1022
BveI ACCTGC 1 cut(s) 628
Cac8I GCNNGC 2 cut(s) 204, 284
CaiI CAGNNNCTG 1 cut(s) 370
CfoI GCGC 1 cut(s) 439
Cfr10I RCCGGY 2 cut(s) 155, 206
CseI GACGC 1 cut(s) 367
Csp6I GTAC 3 cut(s) 566, 688, 739
CviAII CATG 2 cut(s) 779, 936
CviQI GTAC 3 cut(s) 566, 688, 739
DdeI CTNAG 2 cut(s) 371, 772
DpnI GATC 5 cut(s) 502, 573, 756, 804, 882
DpnII GATC 5 cut(s) 500, 571, 754, 802, 880
DraI TTTAAA 1 cut(s) 930
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 3 cut(s) 11, 858, 870
EarI CTCTTC 3 cut(s) 11, 858, 870
Eco130I CCWWGG 1 cut(s) 659
Eco57I CTGAAG 1 cut(s) 512
EcoRII CCWGG 2 cut(s) 183, 574
EcoT14I CCWWGG 1 cut(s) 659
ErhI CCWWGG 1 cut(s) 659
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 2 cut(s) 782, 939
FaqI GGGAC 1 cut(s) 532
FatI CATG 2 cut(s) 778, 935
FauI CCCGC 2 cut(s) 311, 900
FbaI TGATCA 1 cut(s) 754
Fnu4HI GCNGC 4 cut(s) 123, 324, 362, 440
FokI GGATG 4 cut(s) 521, 632, 637, 688
Fsp4HI GCNGC 4 cut(s) 123, 324, 362, 440
GlaI GCGC 1 cut(s) 438
GluI GCNGC 4 cut(s) 123, 324, 362, 440
HaeIII GGCC 1 cut(s) 206
HapII CCGG 4 cut(s) 156, 207, 287, 506
HgaI GACGC 1 cut(s) 367
HhaI GCGC 1 cut(s) 439
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 2 cut(s) 782, 939
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HincII GTYRAC 2 cut(s) 61, 559
HindII GTYRAC 2 cut(s) 61, 559
HinfI GANTC 6 cut(s) 68, 130, 240, 275, 339, 952
HpaI GTTAAC 1 cut(s) 61
HpaII CCGG 4 cut(s) 156, 207, 287, 506
HphI GGTGA 3 cut(s) 77, 133, 284
Hpy166II GTNNAC 4 cut(s) 61, 455, 559, 848
Hpy188I TCNGA 8 cut(s) 213, 239, 304, 372, 390, 775, 786, 802
Hpy188III TCNNGA 4 cut(s) 229, 353, 476, 506
Hpy8I GTNNAC 4 cut(s) 61, 455, 559, 848
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 473, 487
HpyCH4III ACNGT 5 cut(s) 65, 139, 145, 272, 592
HpyCH4V TGCA 2 cut(s) 470, 1027
HpyF10VI GCNNNNNNNGC 1 cut(s) 448
HpyF3I CTNAG 2 cut(s) 371, 772
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 2 cut(s) 782, 939
HspAI GCGC 1 cut(s) 437
Kpn2I TCCGGA 1 cut(s) 505
Ksp22I TGATCA 1 cut(s) 754
KspAI GTTAAC 1 cut(s) 61
Kzo9I GATC 5 cut(s) 500, 571, 754, 802, 880
Lsp1109I GCAGC 1 cut(s) 310
LweI GCATC 4 cut(s) 424, 610, 835, 952
MaeIII GTNAC 6 cut(s) 65, 127, 139, 226, 484, 586
MalI GATC 5 cut(s) 502, 573, 756, 804, 882
MboI GATC 5 cut(s) 500, 571, 754, 802, 880
MboII GAAGA 8 cut(s) 25, 28, 44, 47, 100, 677, 875, 887
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 1024
MluCI AATT 5 cut(s) 298, 613, 793, 978, 984
MlyI GAGTC 5 cut(s) 62, 124, 249, 284, 961
MnlI CCTC 8 cut(s) 241, 435, 736, 781, 808, 871, 904, 964
MroI TCCGGA 1 cut(s) 505
MseI TTAA 6 cut(s) 60, 404, 890, 918, 929, 983
MspA1I CMGCKG 1 cut(s) 609
MspCI CTTAAG 1 cut(s) 403
MspI CCGG 4 cut(s) 156, 207, 287, 506
MspR9I CCNGG 2 cut(s) 185, 576
MvaI CCWGG 2 cut(s) 185, 576
MwoI GCNNNNNNNGC 1 cut(s) 448
NdeII GATC 5 cut(s) 500, 571, 754, 802, 880
NlaIII CATG 2 cut(s) 782, 939
NlaIV GGNNCC 2 cut(s) 160, 769
NmeAIII GCCGAG 1 cut(s) 178
NmuCI GTSAC 5 cut(s) 65, 127, 139, 226, 484
NspI RCATGY 1 cut(s) 939
PciI ACATGT 1 cut(s) 935
PcsI WCGNNNNNNNCGW 1 cut(s) 961
PfeI GAWTC 1 cut(s) 339
Pfl23II CGTACG 1 cut(s) 738
PfoI TCCNGGA 1 cut(s) 574
PkrI GCNGC 4 cut(s) 124, 325, 363, 441
PleI GAGTC 5 cut(s) 62, 124, 248, 283, 960
PpsI GAGTC 5 cut(s) 62, 124, 248, 283, 960
PscI ACATGT 1 cut(s) 935
PsiI TTATAA 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 183, 574
PspGI CCWGG 2 cut(s) 183, 574
PspLI CGTACG 1 cut(s) 738
PspN4I GGNNCC 2 cut(s) 160, 769
PsrI GAACNNNNNNTAC 2 cut(s) 571, 603
PstI CTGCAG 1 cut(s) 1029
PstNI CAGNNNCTG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 500
PvuII CAGCTG 1 cut(s) 609
RsaI GTAC 3 cut(s) 567, 689, 740
RsaNI GTAC 3 cut(s) 566, 688, 739
SaqAI TTAA 6 cut(s) 60, 404, 890, 918, 929, 983
SatI GCNGC 4 cut(s) 123, 324, 362, 440
Sau3AI GATC 5 cut(s) 500, 571, 754, 802, 880
ScaI AGTACT 1 cut(s) 567
SchI GAGTC 5 cut(s) 62, 124, 249, 284, 961
ScrFI CCNGG 2 cut(s) 185, 576
SduI GDGCHC 1 cut(s) 1024
SfaNI GCATC 4 cut(s) 424, 610, 835, 952
SfcI CTRYAG 1 cut(s) 1025
SmlI CTYRAG 3 cut(s) 353, 403, 538
SmoI CTYRAG 3 cut(s) 353, 403, 538
Sse9I AATT 5 cut(s) 298, 613, 793, 978, 984
SspI AATATT 1 cut(s) 998
StyD4I CCNGG 2 cut(s) 183, 574
StyI CCWWGG 1 cut(s) 659
TaaI ACNGT 5 cut(s) 65, 139, 145, 272, 592
TaqI TCGA 2 cut(s) 243, 955
TasI AATT 5 cut(s) 298, 613, 793, 978, 984
TatI WGTACW 1 cut(s) 565
TauI GCSGC 3 cut(s) 125, 364, 442
TfiI GAWTC 1 cut(s) 339
Tru1I TTAA 6 cut(s) 60, 404, 890, 918, 929, 983
Tru9I TTAA 6 cut(s) 60, 404, 890, 918, 929, 983
TscAI CASTG 1 cut(s) 1029
TseFI GTSAC 5 cut(s) 65, 127, 139, 226, 484
TseI GCWGC 1 cut(s) 323
Tsp45I GTSAC 5 cut(s) 65, 127, 139, 226, 484
TspDTI ATGAA 1 cut(s) 327
TspRI CASTG 1 cut(s) 1029
Vha464I CTTAAG 1 cut(s) 403
XapI RAATTY 2 cut(s) 613, 793
XceI RCATGY 1 cut(s) 939
ZrmI AGTACT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.