MD16G1187700.v1.1

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003646
Physical Location & Seq
Forward (+)
16275567 .. 16279496
3930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1187700.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGGGAAGAAGAAGAGAGCCGAATCAGAAGCAGAAGCACAACCAGAAGATAACAAAGTCGACATTGCCAATGGAGATTCTCATAAGAAAGAGAAGAAGAAGAAGCACAAAGTCGACGGGGCCGCCGTGATACGCACCGTGAGCGTCGCCGTCCCCGGTTCAATCATCGATAACACTCAATCCCTCGAACTCGCCACCAGATTGGCCAGTCAGATTGCTCGTGCTGCGACGATTTTCCGAATCGACGAGGTGGTGATATTTGACAATAAGAGTGAGTCTGGCTCATTGCCAGAAGCTGATTCGGATGAGAATGAAAGTGGTGCTGCTTTTCTTGTACGAATTTTGAGGTACCTTGAGACACCTCAGTATTTGAGAAAAGCTCTGTTTCCGAAACACAACAGCTTAAGATATGTGGGTATGTTGCCCCCGCTTGATGCTCCGCACCATCTGCGAAAACATGAATGGGGTCTGTTTCGGGAAGGTTTCACACTGAAAGAAAGACCTCCCAACCTTGTGGGAACACTAGTTGATGTGGGCTTGAGTAAGAATGTCATTGTTGATCAAGTACTTGATCCTGGAACCAGAGTTACTGTGGCTATGGGGGCAAGTTCGAACTTGGATGCTGATATCTCACGGCAGGTTGTCTCATCCTCGAAGCCTAGGGAAGAAGCAGGAACGTATTGGGGGTACAAAGTGCGCTATGCTTCCAATATTACTTCAGTAATGAATGAATGCCCATACAAGGGAGGCTATGATCATTCAATTGGTACATCAGAGCACGGTCAGATTATTAATTCCTCTGATCTCACTATACCTACCTTCAGGCATCTATTGATTGCTTTTGGTGGACTTGCTGGATTGGAAGAGAGCGTTGAAGAGGACGTTAACTTAAAGGCAAAGAATGTGCGAGAGGTATTTGATTTGTATTTGAACACATGCCCGCATCAAGGGAGTCGAACAATTCGAACAGAGGAAGCCATTCTCATATCACTTCAGTATTTCCAAGAACCAATCAGCCGAGCATTGAAGAGAACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

347

Amino Acids

38.37

Weight (kDa)

6.85

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 46 - 333 1.1e-109 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 628
Acc65I GGTACC 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 348
AccI GTMKAC 2 cut(s) 60, 114
AciI CCGC 4 cut(s) 123, 428, 440, 941
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 339
AcuI CTGAAG 3 cut(s) 702, 805, 977
AfaI GTAC 5 cut(s) 336, 350, 567, 689, 769
AfiI CCNNNNNNNGG 3 cut(s) 742, 743, 947
AflII CTTAAG 1 cut(s) 403
AgsI TTSAA 5 cut(s) 162, 762, 875, 931, 1027
AhlI ACTAGT 1 cut(s) 523
AjnI CCWGG 1 cut(s) 574
AluBI AGCT 3 cut(s) 296, 380, 402
AluI AGCT 3 cut(s) 296, 380, 402
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 2 cut(s) 350, 649
AlwI GGATC 1 cut(s) 566
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 2 cut(s) 120, 204
ApeKI GCWGC 2 cut(s) 224, 323
ApoI RAATTY 1 cut(s) 339
AseI ATTAAT 1 cut(s) 792
Asp700I GAANNNNTTC 1 cut(s) 978
Asp718I GGTACC 1 cut(s) 348
AspA2I CCTAGG 1 cut(s) 659
AspLEI GCGC 1 cut(s) 699
AspS9I GGNCC 1 cut(s) 120
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 265
AsuII TTCGAA 2 cut(s) 611, 964
AvrII CCTAGG 1 cut(s) 659
BalI TGGCCA 1 cut(s) 206
BanI GGYRCC 1 cut(s) 348
BauI CACGAG 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 2 cut(s) 211, 310
BccI CCATC 1 cut(s) 453
BceAI ACGGC 3 cut(s) 110, 134, 650
BciT130I CCWGG 1 cut(s) 576
BclI TGATCA 2 cut(s) 559, 754
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 350, 649
BcuI ACTAGT 1 cut(s) 523
BfaI CTAG 2 cut(s) 524, 660
BfrI CTTAAG 1 cut(s) 403
BfuAI ACCTGC 1 cut(s) 628
BisI GCNGC 3 cut(s) 123, 225, 324
BlnI CCTAGG 1 cut(s) 659
BlsI GCNGC 3 cut(s) 124, 226, 325
BmcAI AGTACT 1 cut(s) 567
Bme1390I CCNGG 2 cut(s) 156, 576
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 3 cut(s) 121, 350, 580
BmrFI CCNGG 2 cut(s) 156, 576
BmsI GCATC 4 cut(s) 424, 610, 835, 952
BplI GAGNNNNNCTC 4 cut(s) 266, 298, 364, 396
Bpu14I TTCGAA 2 cut(s) 611, 964
BpuEI CTTGAG 2 cut(s) 374, 559
BpuMI CCSGG 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 154, 659
Bsc4I CCNNNNNNNGG 3 cut(s) 742, 743, 947
Bse1I ACTGG 1 cut(s) 207
Bse3DI GCAATG 2 cut(s) 63, 284
BseBI CCWGG 1 cut(s) 576
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 154, 659
BseGI GGATG 3 cut(s) 310, 625, 647
BseLI CCNNNNNNNGG 3 cut(s) 742, 743, 947
BseMI GCAATG 2 cut(s) 63, 284
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 1 cut(s) 207
BseXI GCAGC 2 cut(s) 211, 310
BshFI GGCC 2 cut(s) 122, 206
BshNI GGYRCC 1 cut(s) 348
BshVI ATCGAT 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 780
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 3 cut(s) 742, 743, 947
BsmAI GTCTC 2 cut(s) 350, 649
BsmFI GGGAC 1 cut(s) 137
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 2 cut(s) 122, 206
Bsp119I TTCGAA 2 cut(s) 611, 964
Bsp1286I GDGCHC 1 cut(s) 780
Bsp143I GATC 4 cut(s) 559, 571, 754, 802
BspACI CCGC 4 cut(s) 123, 428, 440, 941
BspANI GGCC 2 cut(s) 122, 206
BspCNI CTCAG 1 cut(s) 376
BspDI ATCGAT 1 cut(s) 168
BspLI GGNNCC 3 cut(s) 121, 350, 580
BspMI ACCTGC 1 cut(s) 628
BspPI GGATC 1 cut(s) 566
BspT104I TTCGAA 2 cut(s) 611, 964
BspT107I GGYRCC 1 cut(s) 348
BspTI CTTAAG 1 cut(s) 403
BsrDI GCAATG 2 cut(s) 63, 284
BsrI ACTGG 1 cut(s) 207
BssECI CCNNGG 2 cut(s) 154, 659
BssMI GATC 4 cut(s) 559, 571, 754, 802
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 659
Bst2BI CACGAG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 3 cut(s) 139, 592, 782
Bst6I CTCTTC 4 cut(s) 8, 858, 870, 1022
BstAFI CTTAAG 1 cut(s) 403
BstAPI GCANNNNNTGC 1 cut(s) 448
BstBI TTCGAA 2 cut(s) 611, 964
BstC8I GCNNGC 1 cut(s) 941
BstDEI CTNAG 1 cut(s) 363
BstF5I GGATG 3 cut(s) 310, 625, 647
BstHHI GCGC 1 cut(s) 699
BstKTI GATC 4 cut(s) 562, 574, 757, 805
BstMAI GTCTC 2 cut(s) 350, 649
BstMBI GATC 4 cut(s) 559, 571, 754, 802
BstMWI GCNNNNNNNGC 4 cut(s) 141, 224, 448, 602
BstNI CCWGG 1 cut(s) 576
BstNSI RCATGY 1 cut(s) 939
BstSCI CCNGG 2 cut(s) 154, 574
BstV1I GCAGC 2 cut(s) 211, 310
BstXI CCANNNNNNTGG 2 cut(s) 202, 514
Bsu15I ATCGAT 1 cut(s) 168
BsuRI GGCC 2 cut(s) 122, 206
BsuTUI ATCGAT 1 cut(s) 168
BtsCI GGATG 3 cut(s) 310, 625, 647
BtsIMutI CAGTG 1 cut(s) 488
BveI ACCTGC 1 cut(s) 628
Cac8I GCNNGC 1 cut(s) 941
CaiI CAGNNNCTG 1 cut(s) 296
CfoI GCGC 1 cut(s) 699
Cfr13I GGNCC 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 1 cut(s) 133
Csp6I GTAC 5 cut(s) 335, 349, 566, 688, 768
CviAII CATG 2 cut(s) 458, 936
CviQI GTAC 5 cut(s) 335, 349, 566, 688, 768
DdeI CTNAG 1 cut(s) 363
DpnI GATC 4 cut(s) 561, 573, 756, 804
DpnII GATC 4 cut(s) 559, 571, 754, 802
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 4 cut(s) 8, 858, 870, 1022
EarI CTCTTC 4 cut(s) 8, 858, 870, 1022
Eco130I CCWWGG 1 cut(s) 659
Eco32I GATATC 1 cut(s) 628
Eco57I CTGAAG 3 cut(s) 702, 805, 977
EcoRII CCWGG 1 cut(s) 574
EcoRV GATATC 1 cut(s) 628
EcoT14I CCWWGG 1 cut(s) 659
ErhI CCWWGG 1 cut(s) 659
FaeI CATG 2 cut(s) 461, 939
FaqI GGGAC 1 cut(s) 137
FatI CATG 2 cut(s) 457, 935
FauI CCCGC 2 cut(s) 435, 948
FbaI TGATCA 2 cut(s) 559, 754
FblI GTMKAC 2 cut(s) 60, 114
Fnu4HI GCNGC 3 cut(s) 123, 225, 324
FokI GGATG 3 cut(s) 317, 632, 634
Fsp4HI GCNGC 3 cut(s) 123, 225, 324
FspBI CTAG 2 cut(s) 524, 660
GlaI GCGC 1 cut(s) 698
GluI GCNGC 3 cut(s) 123, 225, 324
HaeIII GGCC 2 cut(s) 122, 206
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 699
Hin1II CATG 2 cut(s) 461, 939
Hin6I GCGC 1 cut(s) 697
HinP1I GCGC 1 cut(s) 697
HincII GTYRAC 3 cut(s) 61, 115, 886
HindII GTYRAC 3 cut(s) 61, 115, 886
HinfI GANTC 6 cut(s) 23, 77, 240, 275, 299, 952
HpaI GTTAAC 1 cut(s) 886
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 4 cut(s) 61, 115, 848, 886
Hpy188I TCNGA 8 cut(s) 28, 213, 239, 304, 390, 775, 786, 802
Hpy188III TCNNGA 1 cut(s) 476
Hpy8I GTNNAC 4 cut(s) 61, 115, 848, 886
Hpy99I CGWCG 4 cut(s) 119, 149, 232, 248
HpyAV CCTTC 2 cut(s) 473, 829
HpyCH4III ACNGT 3 cut(s) 139, 592, 782
HpyCH4IV ACGT 2 cut(s) 677, 882
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 224, 448, 602
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 2 cut(s) 677, 882
Hsp92II CATG 2 cut(s) 461, 939
HspAI GCGC 1 cut(s) 697
KpnI GGTACC 1 cut(s) 352
Ksp22I TGATCA 2 cut(s) 559, 754
KspAI GTTAAC 1 cut(s) 886
Kzo9I GATC 4 cut(s) 559, 571, 754, 802
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 2 cut(s) 211, 310
LweI GCATC 4 cut(s) 424, 610, 835, 952
MaeI CTAG 2 cut(s) 524, 660
MaeII ACGT 2 cut(s) 677, 882
MaeIII GTNAC 1 cut(s) 586
MalI GATC 4 cut(s) 561, 573, 756, 804
MboI GATC 4 cut(s) 559, 571, 754, 802
MfeI CAATTG 1 cut(s) 762
MhlI GDGCHC 1 cut(s) 780
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 4 cut(s) 339, 762, 793, 960
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 2 cut(s) 284, 961
Mox20I TGGCCA 1 cut(s) 206
MroXI GAANNNNTTC 1 cut(s) 978
MscI TGGCCA 1 cut(s) 206
MseI TTAA 4 cut(s) 404, 792, 885, 890
Msp20I TGGCCA 1 cut(s) 206
MspCI CTTAAG 1 cut(s) 403
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 156, 576
MunI CAATTG 1 cut(s) 762
Mva1269I GAATGC 1 cut(s) 737
MvaI CCWGG 1 cut(s) 576
MwoI GCNNNNNNNGC 4 cut(s) 141, 224, 448, 602
NciI CCSGG 1 cut(s) 156
NdeII GATC 4 cut(s) 559, 571, 754, 802
NlaIII CATG 2 cut(s) 461, 939
NlaIV GGNNCC 3 cut(s) 121, 350, 580
NspI RCATGY 1 cut(s) 939
NspV TTCGAA 2 cut(s) 611, 964
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 961
PctI GAATGC 1 cut(s) 737
PdmI GAANNNNTTC 1 cut(s) 978
PfeI GAWTC 4 cut(s) 23, 77, 240, 299
PfoI TCCNGGA 1 cut(s) 574
PkrI GCNGC 3 cut(s) 124, 226, 325
PleI GAGTC 2 cut(s) 283, 960
PpsI GAGTC 2 cut(s) 283, 960
PshBI ATTAAT 1 cut(s) 792
Psp6I CCWGG 1 cut(s) 574
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 3 cut(s) 121, 350, 580
PspPI GGNCC 1 cut(s) 120
PsrI GAACNNNNNNTAC 2 cut(s) 571, 603
PstNI CAGNNNCTG 1 cut(s) 296
RsaI GTAC 5 cut(s) 336, 350, 567, 689, 769
RsaNI GTAC 5 cut(s) 335, 349, 566, 688, 768
SalI GTCGAC 2 cut(s) 59, 113
SaqAI TTAA 4 cut(s) 404, 792, 885, 890
SatI GCNGC 3 cut(s) 123, 225, 324
Sau3AI GATC 4 cut(s) 559, 571, 754, 802
Sau96I GGNCC 1 cut(s) 120
ScaI AGTACT 1 cut(s) 567
SchI GAGTC 2 cut(s) 284, 961
ScrFI CCNGG 2 cut(s) 156, 576
SduI GDGCHC 1 cut(s) 780
SfaNI GCATC 4 cut(s) 424, 610, 835, 952
SfuI TTCGAA 2 cut(s) 611, 964
SmlI CTYRAG 3 cut(s) 353, 403, 538
SmoI CTYRAG 3 cut(s) 353, 403, 538
SpeI ACTAGT 1 cut(s) 523
Sse9I AATT 4 cut(s) 339, 762, 793, 960
SsiI CCGC 4 cut(s) 123, 428, 440, 941
SspI AATATT 1 cut(s) 712
SspMI CTAG 2 cut(s) 524, 660
StyD4I CCNGG 2 cut(s) 154, 574
StyI CCWWGG 1 cut(s) 659
TaaI ACNGT 3 cut(s) 139, 592, 782
TaiI ACGT 2 cut(s) 680, 885
TaqI TCGA 9 cut(s) 60, 114, 168, 186, 243, 611, 653, 955, 964
TasI AATT 4 cut(s) 339, 762, 793, 960
TatI WGTACW 1 cut(s) 565
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 4 cut(s) 23, 77, 240, 299
Tru1I TTAA 4 cut(s) 404, 792, 885, 890
Tru9I TTAA 4 cut(s) 404, 792, 885, 890
TscAI CASTG 1 cut(s) 495
TseI GCWGC 2 cut(s) 224, 323
TspDTI ATGAA 4 cut(s) 327, 474, 740, 744
TspRI CASTG 1 cut(s) 495
Vha464I CTTAAG 1 cut(s) 403
VspI ATTAAT 1 cut(s) 792
XapI RAATTY 1 cut(s) 339
XceI RCATGY 1 cut(s) 939
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmaJI CCTAGG 1 cut(s) 659
XmiI GTMKAC 2 cut(s) 60, 114
XmnI GAANNNNTTC 1 cut(s) 978
XspI CTAG 2 cut(s) 524, 660
ZrmI AGTACT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.