FvH4_4g14960

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003646
Physical Location & Seq
Forward (+)
18558790 .. 18562252
3463 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14960.t2

Sequence Viewer

Length: 1023 bp
ATGGGCAAAAAGAATAAGAGAACCGAAGCGGAAGCACCACCCCAACCTGAAATCAAACCCGACGAGAAGAAGAAGAAGAGCAAGAAACCCAAACCCGACACATCCCCCGCCGTGATTCCCACCGTCACCGTCGCCATCGCCGGCTCCATCATCCACAACGCTCAATCCATCGAACTCGCCACTCGGCTCGCCGGTCAGATTGCTCGTGCCGTCACGATTTTCCGAGTCGACGAGGTGGTTGTGTTTGACAATAACGGTGAGACTGGCCCGCCGGAAGATACTAATTCCGATGAGAATGAAAGCGGGGCTGCATTTCTTATACGGATCTTGCGGTACCTCGAGACGCCGCAATATTTGCGAAAAGCTCTGTTTCCGAAGCATAATAGCTTAAGATTTGTGGGTTTGTTGCCTCCGCTTGACGCGCCGCACCATCTGCGTAAGCACGAATGGGGTGTATATCGGGAAGGTGCCACACTGAAGGAAAGATCTCCAGATGGTGTTGGGACATTGGTTGATGTGGGCTTGAGTAAGAATGTCGTTGTTGATCAAGTACTTGATCCTGGAACAAGAGTAACTGTAGCTATGGGAGACAGTTGGAATTTGGATGCTGATATATCACGGCAGGTTGTATCATCGTCCAAGCCAAGGGAAGAAGCAGGGATGTATTGGGGGTACAAAGTGCGCTATGCTTCCAATATCACTTCGGTAATGACTGAATGTCCGTACGAGGGTGGCTATGATCATTTGATTGGAACCTCAGAGCATGGTCAAATTATCAGTTCTTCTGATCTCACTATACCTACGTTCAGGCATCTATTGATTGCTTTTGGTGGACTTGCTGGCTTGGAAGAGAGCATTGAAGAGGATCATAACTTAAAGGAAAAGAATGTTCGGGAGGTGTTTGATATATATTTAAACACATGTCCGCATCAGGGGAGTCGAACAATACGAACCGAGGAAGCGATTTTAATTTCGCTTCAGTATTTCCAAGAGCCAATCAACAGAGCAGTGCAGAGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

341

Amino Acids

37.76

Weight (kDa)

6.1

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 41 - 265 1.3e-82 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 613
Acc65I GGTACC 1 cut(s) 333
AccB1I GGYRCC 2 cut(s) 333, 467
AccI GTMKAC 1 cut(s) 228
AccII CGCG 1 cut(s) 422
AciI CCGC 9 cut(s) 29, 108, 269, 303, 331, 347, 413, 425, 926
AclWI GGATC 3 cut(s) 332, 551, 873
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 2 cut(s) 497, 962
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 4 cut(s) 335, 552, 674, 725
AfiI CCNNNNNNNGG 3 cut(s) 645, 728, 932
AflII CTTAAG 1 cut(s) 388
AflIII ACRYGT 1 cut(s) 920
AgsI TTSAA 1 cut(s) 860
AhdI GACNNNNNGTC 1 cut(s) 717
AjnI CCWGG 1 cut(s) 559
AluBI AGCT 3 cut(s) 365, 387, 581
AluI AGCT 3 cut(s) 365, 387, 581
Alw26I GTCTC 3 cut(s) 254, 335, 582
AlwI GGATC 3 cut(s) 332, 551, 873
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 1 cut(s) 308
ApoI RAATTY 1 cut(s) 598
Asp718I GGTACC 1 cut(s) 333
AspLEI GCGC 2 cut(s) 424, 684
AspS9I GGNCC 1 cut(s) 266
AsuHPI GGTGA 2 cut(s) 118, 269
AvaI CYCGRG 1 cut(s) 338
BanI GGYRCC 2 cut(s) 333, 467
BauI CACGAG 1 cut(s) 204
BbvI GCAGC 1 cut(s) 295
BccI CCATC 5 cut(s) 143, 155, 176, 438, 488
BceAI ACGGC 3 cut(s) 95, 194, 635
BciT130I CCWGG 1 cut(s) 561
BclI TGATCA 2 cut(s) 544, 739
BcoDI GTCTC 3 cut(s) 254, 335, 582
BfmI CTRYAG 1 cut(s) 576
BfrI CTTAAG 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 613
BglII AGATCT 1 cut(s) 485
BisI GCNGC 3 cut(s) 309, 347, 425
BlsI GCNGC 3 cut(s) 310, 348, 426
BmcAI AGTACT 1 cut(s) 552
Bme1390I CCNGG 1 cut(s) 561
BmeRI GACNNNNNGTC 1 cut(s) 717
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 4 cut(s) 145, 335, 469, 754
BmrFI CCNGG 1 cut(s) 561
BmsI GCATC 3 cut(s) 595, 820, 937
BpmI CTGGAG 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 544
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 644, 954
Bsc4I CCNNNNNNNGG 3 cut(s) 645, 728, 932
Bse118I RCCGGY 2 cut(s) 140, 191
Bse1I ACTGG 1 cut(s) 268
BseBI CCWGG 1 cut(s) 561
BseDI CCNNGG 2 cut(s) 644, 954
BseGI GGATG 4 cut(s) 101, 150, 610, 666
BseLI CCNNNNNNNGG 3 cut(s) 645, 728, 932
BseMII CTCAG 1 cut(s) 771
BseNI ACTGG 1 cut(s) 268
BseXI GCAGC 1 cut(s) 295
Bsh1236I CGCG 1 cut(s) 422
BshFI GGCC 1 cut(s) 267
BshNI GGYRCC 2 cut(s) 333, 467
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 3 cut(s) 141, 192, 272
BsiWI CGTACG 1 cut(s) 723
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 3 cut(s) 645, 728, 932
BsmAI GTCTC 3 cut(s) 254, 335, 582
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 517
BsnI GGCC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 338
Bsp143I GATC 7 cut(s) 324, 485, 544, 556, 739, 787, 865
BspACI CCGC 9 cut(s) 29, 108, 269, 303, 331, 347, 413, 425, 926
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 1 cut(s) 770
BspFNI CGCG 1 cut(s) 422
BspLI GGNNCC 4 cut(s) 145, 335, 469, 754
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 3 cut(s) 332, 551, 873
BspQI GCTCTTC 1 cut(s) 71
BspT107I GGYRCC 2 cut(s) 333, 467
BspTI CTTAAG 1 cut(s) 388
BsrFI RCCGGY 2 cut(s) 140, 191
BsrI ACTGG 1 cut(s) 268
BssAI RCCGGY 2 cut(s) 140, 191
BssECI CCNNGG 2 cut(s) 644, 954
BssMI GATC 7 cut(s) 324, 485, 544, 556, 739, 787, 865
BssNI GRCGYC 1 cut(s) 344
BssSI CACGAG 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 644
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 5 cut(s) 124, 130, 257, 577, 593
Bst6I CTCTTC 3 cut(s) 71, 843, 855
BstACI GRCGYC 1 cut(s) 344
BstAFI CTTAAG 1 cut(s) 388
BstAPI GCANNNNNTGC 2 cut(s) 355, 433
BstC8I GCNNGC 4 cut(s) 142, 189, 269, 841
BstDEI CTNAG 1 cut(s) 757
BstF5I GGATG 4 cut(s) 101, 150, 610, 666
BstFNI CGCG 1 cut(s) 422
BstHHI GCGC 2 cut(s) 424, 684
BstKTI GATC 7 cut(s) 327, 488, 547, 559, 742, 790, 868
BstMAI GTCTC 3 cut(s) 254, 335, 582
BstMBI GATC 7 cut(s) 324, 485, 544, 556, 739, 787, 865
BstMWI GCNNNNNNNGC 3 cut(s) 355, 421, 433
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 1 cut(s) 924
BstSCI CCNGG 1 cut(s) 559
BstSFI CTRYAG 1 cut(s) 576
BstUI CGCG 1 cut(s) 422
BstV1I GCAGC 1 cut(s) 295
BstX2I RGATCY 2 cut(s) 324, 485
BstYI RGATCY 2 cut(s) 324, 485
BsuRI GGCC 1 cut(s) 267
BtgZI GCGATG 1 cut(s) 121
BtsCI GGATG 4 cut(s) 101, 150, 610, 666
BtsI GCAGTG 1 cut(s) 1014
BtsIMutI CAGTG 2 cut(s) 473, 1014
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 4 cut(s) 142, 189, 269, 841
CfoI GCGC 2 cut(s) 424, 684
Cfr10I RCCGGY 2 cut(s) 140, 191
Cfr13I GGNCC 1 cut(s) 266
CseI GACGC 2 cut(s) 352, 428
Csp6I GTAC 4 cut(s) 334, 551, 673, 724
CviAII CATG 2 cut(s) 764, 921
CviQI GTAC 4 cut(s) 334, 551, 673, 724
DdeI CTNAG 1 cut(s) 757
DpnI GATC 7 cut(s) 326, 487, 546, 558, 741, 789, 867
DpnII GATC 7 cut(s) 324, 485, 544, 556, 739, 787, 865
DraI TTTAAA 1 cut(s) 915
DriI GACNNNNNGTC 1 cut(s) 717
Eam1104I CTCTTC 3 cut(s) 71, 843, 855
Eam1105I GACNNNNNGTC 1 cut(s) 717
EarI CTCTTC 3 cut(s) 71, 843, 855
Eco130I CCWWGG 1 cut(s) 644
Eco57I CTGAAG 2 cut(s) 497, 962
Eco88I CYCGRG 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 559
EcoT14I CCWWGG 1 cut(s) 644
ErhI CCWWGG 1 cut(s) 644
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 2 cut(s) 767, 924
FaqI GGGAC 1 cut(s) 517
FatI CATG 2 cut(s) 763, 920
FauI CCCGC 3 cut(s) 115, 276, 296
FbaI TGATCA 2 cut(s) 544, 739
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 3 cut(s) 309, 347, 425
FokI GGATG 4 cut(s) 88, 137, 617, 673
Fsp4HI GCNGC 3 cut(s) 309, 347, 425
GlaI GCGC 2 cut(s) 423, 683
GluI GCNGC 3 cut(s) 309, 347, 425
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 1 cut(s) 267
HapII CCGG 3 cut(s) 141, 192, 272
HgaI GACGC 2 cut(s) 352, 428
HhaI GCGC 2 cut(s) 424, 684
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 2 cut(s) 767, 924
Hin6I GCGC 2 cut(s) 422, 682
HinP1I GCGC 2 cut(s) 422, 682
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 3 cut(s) 115, 225, 937
HpaII CCGG 3 cut(s) 141, 192, 272
HphI GGTGA 2 cut(s) 118, 269
Hpy166II GTNNAC 2 cut(s) 229, 833
Hpy188I TCNGA 6 cut(s) 198, 224, 289, 375, 760, 787
Hpy188III TCNNGA 5 cut(s) 214, 340, 461, 491, 893
Hpy8I GTNNAC 2 cut(s) 229, 833
Hpy99I CGWCG 3 cut(s) 65, 134, 233
HpyAV CCTTC 2 cut(s) 458, 472
HpyCH4III ACNGT 5 cut(s) 124, 130, 257, 577, 593
HpyCH4IV ACGT 1 cut(s) 803
HpyCH4V TGCA 2 cut(s) 311, 1012
HpyF10VI GCNNNNNNNGC 3 cut(s) 355, 421, 433
HpyF3I CTNAG 1 cut(s) 757
HpySE526I ACGT 1 cut(s) 803
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 2 cut(s) 767, 924
HspAI GCGC 2 cut(s) 422, 682
KpnI GGTACC 1 cut(s) 337
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Ksp22I TGATCA 2 cut(s) 544, 739
Kzo9I GATC 7 cut(s) 324, 485, 544, 556, 739, 787, 865
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 1 cut(s) 295
LweI GCATC 3 cut(s) 595, 820, 937
MaeII ACGT 1 cut(s) 803
MaeIII GTNAC 3 cut(s) 124, 211, 571
MalI GATC 7 cut(s) 326, 487, 546, 558, 741, 789, 867
MboI GATC 7 cut(s) 324, 485, 544, 556, 739, 787, 865
MboII GAAGA 9 cut(s) 79, 82, 85, 88, 287, 662, 774, 860, 872
MflI RGATCY 2 cut(s) 324, 485
MluCI AATT 4 cut(s) 283, 598, 771, 969
MlyI GAGTC 2 cut(s) 234, 946
MmeI TCCRAC 1 cut(s) 575
MnlI CCTC 8 cut(s) 226, 347, 420, 721, 766, 856, 889, 949
MroNI GCCGGC 1 cut(s) 140
MseI TTAA 4 cut(s) 389, 875, 914, 968
MspCI CTTAAG 1 cut(s) 388
MspI CCGG 3 cut(s) 141, 192, 272
MspR9I CCNGG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 561
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 3 cut(s) 355, 421, 433
NaeI GCCGGC 1 cut(s) 142
NdeII GATC 7 cut(s) 324, 485, 544, 556, 739, 787, 865
NgoMIV GCCGGC 1 cut(s) 140
NlaIII CATG 2 cut(s) 767, 924
NlaIV GGNNCC 4 cut(s) 145, 335, 469, 754
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 2 cut(s) 124, 211
NspI RCATGY 1 cut(s) 924
PaeR7I CTCGAG 1 cut(s) 338
PciI ACATGT 1 cut(s) 920
PciSI GCTCTTC 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 946
PdiI GCCGGC 1 cut(s) 142
PfeI GAWTC 1 cut(s) 115
Pfl23II CGTACG 1 cut(s) 723
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 3 cut(s) 310, 348, 426
PleI GAGTC 2 cut(s) 233, 945
PpsI GAGTC 2 cut(s) 233, 945
PscI ACATGT 1 cut(s) 920
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspLI CGTACG 1 cut(s) 723
PspN4I GGNNCC 4 cut(s) 145, 335, 469, 754
PspPI GGNCC 1 cut(s) 266
PsuI RGATCY 2 cut(s) 324, 485
RsaI GTAC 4 cut(s) 335, 552, 674, 725
RsaNI GTAC 4 cut(s) 334, 551, 673, 724
SalI GTCGAC 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 4 cut(s) 389, 875, 914, 968
SatI GCNGC 3 cut(s) 309, 347, 425
Sau3AI GATC 7 cut(s) 324, 485, 544, 556, 739, 787, 865
Sau96I GGNCC 1 cut(s) 266
ScaI AGTACT 1 cut(s) 552
SchI GAGTC 2 cut(s) 234, 946
ScrFI CCNGG 1 cut(s) 561
SfaNI GCATC 3 cut(s) 595, 820, 937
SfcI CTRYAG 1 cut(s) 576
Sfr274I CTCGAG 1 cut(s) 338
SlaI CTCGAG 1 cut(s) 338
SmlI CTYRAG 3 cut(s) 338, 388, 523
SmoI CTYRAG 3 cut(s) 338, 388, 523
Sse9I AATT 4 cut(s) 283, 598, 771, 969
SsiI CCGC 9 cut(s) 29, 108, 269, 303, 331, 347, 413, 425, 926
SspI AATATT 1 cut(s) 353
StyD4I CCNGG 1 cut(s) 559
StyI CCWWGG 1 cut(s) 644
TaaI ACNGT 5 cut(s) 124, 130, 257, 577, 593
TaiI ACGT 1 cut(s) 806
TaqI TCGA 4 cut(s) 171, 228, 339, 940
TasI AATT 4 cut(s) 283, 598, 771, 969
TatI WGTACW 1 cut(s) 550
TauI GCSGC 2 cut(s) 349, 427
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 4 cut(s) 389, 875, 914, 968
Tru9I TTAA 4 cut(s) 389, 875, 914, 968
TscAI CASTG 2 cut(s) 480, 1014
TseFI GTSAC 2 cut(s) 124, 211
TseI GCWGC 1 cut(s) 308
Tsp45I GTSAC 2 cut(s) 124, 211
TspDTI ATGAA 1 cut(s) 312
TspGWI ACGGA 2 cut(s) 337, 711
TspRI CASTG 2 cut(s) 480, 1014
Vha464I CTTAAG 1 cut(s) 388
XapI RAATTY 1 cut(s) 598
XceI RCATGY 1 cut(s) 924
XhoI CTCGAG 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 228
ZrmI AGTACT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.