Rh6DG155500

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003646
Physical Location & Seq
Reverse (-)
23373321 .. 23388921
15601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG155500.1

Sequence Viewer

Length: 561 bp
ATGGAGGTCGCGACATTCGTGCTGTGCTGGTGGGACGATATCAACGAGTCGACTCAGTGGCAAGACGACATCTTTTACGCTCTCTGCACAGCCGAACCCCCTCGACTCCTCCGTCGCTCTGGTGTCACACTAAAGGAAAGATCTCCAGACGGTGTTGGGACGTTAGTTGATGTGGGCTTGAGTAAGAATGTCGTTGTTGATCAAGTACTTGATCCTAGAACAAGAGTTACAGTGGCTATGGGAGACAGTTGGAATTTGGATGCTGATATATCACAGCAGGTTGGATCATCATCCAAGCCAAGGGAAGAAGCAGGGATGTATTGGGGGTACAAAGTGCACTATGCTTCCAATATCACTTCGGTAATGATTGAATCTCCATACGAGCATGAGAGCTTGAAGGAACAGCATGAGCTTCTCCAGAAAGTAGTGAAGCCTTTGACGGGTCATTTAGAGATCTTAGTACCCAATGGTGGCCTTAACACTCACCGTTCTTCAGGTAACATTGTCTTCATAATTGGTGCAGATCTTGTTGGAGGAGATGTCTTTGAAGGAGGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

186

Amino Acids

20.51

Weight (kDa)

4.77

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 39 - 129 1.5e-18 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 111
Acc36I ACCTGC 1 cut(s) 268
AccI GTMKAC 1 cut(s) 50
AccII CGCG 1 cut(s) 11
AclWI GGATC 2 cut(s) 206, 292
AcsI RAATTY 1 cut(s) 253
AcuI CTGAAG 1 cut(s) 477
AfaI GTAC 3 cut(s) 207, 329, 462
AfiI CCNNNNNNNGG 3 cut(s) 300, 440, 470
AgsI TTSAA 3 cut(s) 371, 397, 548
AluBI AGCT 2 cut(s) 393, 412
AluI AGCT 2 cut(s) 393, 412
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 237
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 2 cut(s) 206, 292
AoxI GGCC 2 cut(s) 472, 555
ApaLI GTGCAC 1 cut(s) 335
ApoI RAATTY 1 cut(s) 253
AspS9I GGNCC 1 cut(s) 555
AsuHPI GGTGA 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 499
Bbv12I GWGCWC 1 cut(s) 339
BcgI CGANNNNNNTGC 1 cut(s) 35
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 237
BfaI CTAG 2 cut(s) 216, 559
BfuAI ACCTGC 1 cut(s) 268
BglII AGATCT 3 cut(s) 140, 453, 523
BmcAI AGTACT 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 555
BmiI GGNNCC 1 cut(s) 556
BmsI GCATC 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 499
BpmI CTGGAG 2 cut(s) 129, 401
BpuEI CTTGAG 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 289
BsaJI CCNNGG 1 cut(s) 299
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 3 cut(s) 300, 440, 470
Bse8I GATNNNNATC 1 cut(s) 289
BseDI CCNNGG 1 cut(s) 299
BseGI GGATG 3 cut(s) 265, 290, 321
BseJI GATNNNNATC 1 cut(s) 289
BseLI CCNNNNNNNGG 3 cut(s) 300, 440, 470
BseMII CTCAG 1 cut(s) 68
BseRI GAGGAG 2 cut(s) 98, 549
BseSI GKGCMC 1 cut(s) 339
BsgI GTGCAG 2 cut(s) 70, 540
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 2 cut(s) 474, 557
BsiHKAI GWGCWC 1 cut(s) 339
BslFI GGGAC 2 cut(s) 47, 172
BslI CCNNNNNNNGG 3 cut(s) 300, 440, 470
BsmAI GTCTC 1 cut(s) 237
BsmFI GGGAC 2 cut(s) 47, 172
BsnI GGCC 2 cut(s) 474, 557
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 6 cut(s) 140, 199, 211, 284, 453, 523
Bsp68I TCGCGA 1 cut(s) 11
BspANI GGCC 2 cut(s) 474, 557
BspCNI CTCAG 1 cut(s) 67
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 2 cut(s) 206, 292
BssECI CCNNGG 1 cut(s) 299
BssMI GATC 6 cut(s) 140, 199, 211, 284, 453, 523
BssT1I CCWWGG 1 cut(s) 299
Bst4CI ACNGT 4 cut(s) 152, 232, 248, 488
BstDEI CTNAG 2 cut(s) 54, 457
BstF5I GGATG 3 cut(s) 265, 290, 321
BstFNI CGCG 1 cut(s) 11
BstKTI GATC 6 cut(s) 143, 202, 214, 287, 456, 526
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 6 cut(s) 140, 199, 211, 284, 453, 523
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 3 cut(s) 140, 453, 523
BstYI RGATCY 3 cut(s) 140, 453, 523
BsuRI GGCC 2 cut(s) 474, 557
BtsCI GGATG 3 cut(s) 265, 290, 321
BtsIMutI CAGTG 2 cut(s) 62, 237
BtuMI TCGCGA 1 cut(s) 11
BveI ACCTGC 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 555
Csp6I GTAC 3 cut(s) 206, 328, 461
CviAII CATG 2 cut(s) 386, 407
CviJI RGCY 9 cut(s) 92, 177, 236, 298, 393, 412, 433, 474, 557
CviKI_1 RGCY 9 cut(s) 92, 177, 236, 298, 393, 412, 433, 474, 557
CviQI GTAC 3 cut(s) 206, 328, 461
DdeI CTNAG 2 cut(s) 54, 457
DpnI GATC 6 cut(s) 142, 201, 213, 286, 455, 525
DpnII GATC 6 cut(s) 140, 199, 211, 284, 453, 523
DrdI GACNNNNNNGTC 1 cut(s) 111
DseDI GACNNNNNNGTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 299
Eco32I GATATC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 477
EcoO109I RGGNCCY 1 cut(s) 555
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 2 cut(s) 389, 410
FaiI YATR 7 cut(s) 239, 269, 342, 379, 387, 408, 512
FaqI GGGAC 2 cut(s) 47, 172
FatI CATG 2 cut(s) 385, 406
FbaI TGATCA 1 cut(s) 199
FblI GTMKAC 1 cut(s) 50
FokI GGATG 3 cut(s) 272, 277, 328
FspBI CTAG 2 cut(s) 216, 559
GsuI CTGGAG 2 cut(s) 129, 401
HaeIII GGCC 2 cut(s) 474, 557
Hin1II CATG 2 cut(s) 389, 410
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 4 cut(s) 47, 52, 105, 371
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 2 cut(s) 51, 337
Hpy188III TCNNGA 3 cut(s) 10, 146, 418
Hpy8I GTNNAC 2 cut(s) 51, 337
Hpy99I CGWCG 1 cut(s) 117
HpyAV CCTTC 2 cut(s) 391, 542
HpyCH4III ACNGT 4 cut(s) 152, 232, 248, 488
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 3 cut(s) 87, 337, 521
HpyF3I CTNAG 2 cut(s) 54, 457
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 2 cut(s) 389, 410
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 6 cut(s) 140, 199, 211, 284, 453, 523
LpnPI CCDG 7 cut(s) 13, 105, 159, 263, 297, 431, 480
LweI GCATC 1 cut(s) 250
MaeI CTAG 2 cut(s) 216, 559
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 3 cut(s) 124, 226, 497
MalI GATC 6 cut(s) 142, 201, 213, 286, 455, 525
MboI GATC 6 cut(s) 140, 199, 211, 284, 453, 523
MboII GAAGA 3 cut(s) 317, 483, 499
MflI RGATCY 3 cut(s) 140, 453, 523
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 2 cut(s) 253, 513
MlyI GAGTC 3 cut(s) 46, 56, 99
MmeI TCCRAC 3 cut(s) 230, 262, 511
MnlI CCTC 4 cut(s) 111, 119, 527, 545
MseI TTAA 1 cut(s) 477
MvnI CGCG 1 cut(s) 11
NdeII GATC 6 cut(s) 140, 199, 211, 284, 453, 523
NlaIII CATG 2 cut(s) 389, 410
NlaIV GGNNCC 1 cut(s) 556
NmuCI GTSAC 1 cut(s) 124
NruI TCGCGA 1 cut(s) 11
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 42, 109
PfeI GAWTC 1 cut(s) 371
PleI GAGTC 3 cut(s) 46, 55, 99
PpsI GAGTC 3 cut(s) 46, 55, 99
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 1 cut(s) 555
PsrI GAACNNNNNNTAC 2 cut(s) 211, 243
PsuI RGATCY 3 cut(s) 140, 453, 523
RruI TCGCGA 1 cut(s) 11
RsaI GTAC 3 cut(s) 207, 329, 462
RsaNI GTAC 3 cut(s) 206, 328, 461
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 477
Sau3AI GATC 6 cut(s) 140, 199, 211, 284, 453, 523
Sau96I GGNCC 1 cut(s) 555
ScaI AGTACT 1 cut(s) 207
SchI GAGTC 3 cut(s) 46, 56, 99
SduI GDGCHC 1 cut(s) 339
SetI ASST 6 cut(s) 9, 164, 282, 395, 414, 499
SfaNI GCATC 1 cut(s) 250
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse9I AATT 2 cut(s) 253, 513
SspMI CTAG 2 cut(s) 216, 559
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 4 cut(s) 152, 232, 248, 488
TaiI ACGT 1 cut(s) 164
TaqI TCGA 2 cut(s) 50, 103
TasI AATT 2 cut(s) 253, 513
TatI WGTACW 1 cut(s) 205
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 2 cut(s) 62, 237
TseFI GTSAC 1 cut(s) 124
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 1 cut(s) 499
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 2 cut(s) 62, 237
VneI GTGCAC 1 cut(s) 335
XapI RAATTY 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 50
XspI CTAG 2 cut(s) 216, 559
ZrmI AGTACT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.