RchiOBHm_Chr4g0417131

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003646
Physical Location & Seq
Reverse (-)
42334233 .. 42338082
3850 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38722

Sequence Viewer

Length: 1044 bp
ATGGGTAAAAAGAAGAAGAGAACCGAAGCGGAAGAAGAAATCAAAGCCGACGACATTGTTAACGGCGACTTGCATAACAAAACCAACAAGAAGAAGAAGAAGAAGAAGGAACCCAAAACCGACACAGCCGCCGTGATTCCCACCGTCACCGTAGCCATCGCCGGTTCCATCATTCACAACGCTCAATCCCTCGAACTCGCCACTCGGCTGGCCGGTCAGATAGCTCGTGCCGTCACGATTTTCCGAGTTGATGAGGTGGTGGTATTTGACAACAACGGTGAGTCTGGCTCGCCGGAAGCTGATAATTCCGATGAGAATGAAAGCGGGGCTGCTTTTCTTATAAGAATCTTGCGGTATCTTGAGACGCCGCAGTATCTGAGGAAAGCTCTATTTCCGAAACATAATAGCTTGAGATTTGTGGGCTTGTTGCCTCCTCTTGATGCGCCGCACCATCTGCGTAGGCACGAATGGGGTGCATTTCGGGAAGGTGTCACACTAAAGGAAAGATCTCTGGATGGTGTTGGGACATTAGTTGATGTGGGCTTGAGTAAGAATGTTGTCGTTGACCAAGTACTTGATCCTGGAACAAGAGTTACTGTGGCTATGGGAGACAGCTGGAATTTGGATGCTGATGTATCACGGCAGGTTGTATCATCGTTCAAGCCAAGGGAAGAAGCAGGGATGTATTGGGGGTACAAAGTGCGGTATGCTTCCAATATCACTTCGGTAATGACTGAATGTCTGTACGAGGGTGGTTATGATCATTTGATTGGAACCTCAGAGCATGGTCAGATTATAAATTCCTCTGATCTCAATATACCTACTTTCAGGCATCTATTGATTGCTTTCGGTGGACTTGCTGGATTGGAAGAGAGCATTGAAGAGGATCATAACTTAAAGGCAAAGAATGTGCGGGAGGTGTTTGATATATATTTAAACACATGTCCGCATCAGGGGAGTCGAACAATACGAACAGAGGAAGCAATTTTAATTTCACTTCAATATTTCCAAGAACCAATCAACAGAGCACTGCAGAGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

347

Amino Acids

38.67

Weight (kDa)

6.28

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 48 - 335 6.7e-108 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 341, 797
Acc36I ACCTGC 1 cut(s) 634
AciI CCGC 9 cut(s) 29, 129, 324, 352, 368, 446, 703, 913, 947
AclWI GGATC 2 cut(s) 572, 894
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 2 cut(s) 619, 799
AcyI GRCGYC 1 cut(s) 365
AfaI GTAC 3 cut(s) 573, 695, 746
AfiI CCNNNNNNNGG 1 cut(s) 953
AflIII ACRYGT 1 cut(s) 941
AgsI TTSAA 3 cut(s) 661, 881, 1001
AhdI GACNNNNNGTC 1 cut(s) 738
AjnI CCWGG 1 cut(s) 580
AluBI AGCT 5 cut(s) 224, 299, 386, 408, 615
AluI AGCT 5 cut(s) 224, 299, 386, 408, 615
Alw21I GWGCWC 1 cut(s) 1030
Alw26I GTCTC 2 cut(s) 356, 603
AlwI GGATC 2 cut(s) 572, 894
AlwNI CAGNNNCTG 1 cut(s) 376
AoxI GGCC 1 cut(s) 210
ApeKI GCWGC 1 cut(s) 329
ApoI RAATTY 2 cut(s) 619, 799
AspLEI GCGC 1 cut(s) 445
AsuHPI GGTGA 2 cut(s) 139, 290
BauI CACGAG 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 1030
BbvI GCAGC 1 cut(s) 316
BccI CCATC 4 cut(s) 164, 176, 459, 509
BceAI ACGGC 4 cut(s) 79, 116, 215, 656
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BciT130I CCWGG 1 cut(s) 582
BclI TGATCA 1 cut(s) 760
BcoDI GTCTC 2 cut(s) 356, 603
BfmI CTRYAG 1 cut(s) 1031
BfuAI ACCTGC 1 cut(s) 634
BglII AGATCT 1 cut(s) 506
BisI GCNGC 4 cut(s) 129, 330, 368, 446
BlsI GCNGC 4 cut(s) 130, 331, 369, 447
BmcAI AGTACT 1 cut(s) 573
Bme1390I CCNGG 1 cut(s) 582
BmeRI GACNNNNNGTC 1 cut(s) 738
BmiI GGNNCC 3 cut(s) 111, 166, 775
BmrFI CCNGG 1 cut(s) 582
BmsI GCATC 4 cut(s) 430, 616, 841, 958
BplI GAGNNNNNCTC 4 cut(s) 272, 304, 370, 402
BpuEI CTTGAG 3 cut(s) 380, 430, 565
BsaHI GRCGYC 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 665
Bsc4I CCNNNNNNNGG 1 cut(s) 953
Bse118I RCCGGY 2 cut(s) 161, 212
BseBI CCWGG 1 cut(s) 582
BseDI CCNNGG 1 cut(s) 665
BseGI GGATG 3 cut(s) 520, 631, 687
BseLI CCNNNNNNNGG 1 cut(s) 953
BseMII CTCAG 2 cut(s) 368, 792
BseRI GAGGAG 1 cut(s) 423
BseXI GCAGC 1 cut(s) 316
BshFI GGCC 1 cut(s) 212
BsiHKAI GWGCWC 1 cut(s) 1030
BsiSI CCGG 3 cut(s) 162, 213, 293
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 1 cut(s) 953
BsmAI GTCTC 2 cut(s) 356, 603
BsmBI CGTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 538
BsnI GGCC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 1030
Bsp143I GATC 5 cut(s) 506, 577, 760, 808, 886
BspACI CCGC 9 cut(s) 29, 129, 324, 352, 368, 446, 703, 913, 947
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 2 cut(s) 369, 791
BspLI GGNNCC 3 cut(s) 111, 166, 775
BspMAI CTGCAG 1 cut(s) 1035
BspMI ACCTGC 1 cut(s) 634
BspPI GGATC 2 cut(s) 572, 894
BsrFI RCCGGY 2 cut(s) 161, 212
BssAI RCCGGY 2 cut(s) 161, 212
BssECI CCNNGG 1 cut(s) 665
BssMI GATC 5 cut(s) 506, 577, 760, 808, 886
BssNI GRCGYC 1 cut(s) 365
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 665
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 4 cut(s) 145, 151, 278, 598
Bst6I CTCTTC 3 cut(s) 11, 864, 876
BstACI GRCGYC 1 cut(s) 365
BstAPI GCANNNNNTGC 1 cut(s) 454
BstC8I GCNNGC 2 cut(s) 210, 290
BstDEI CTNAG 2 cut(s) 377, 778
BstF5I GGATG 3 cut(s) 520, 631, 687
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 5 cut(s) 509, 580, 763, 811, 889
BstMAI GTCTC 2 cut(s) 356, 603
BstMBI GATC 5 cut(s) 506, 577, 760, 808, 886
BstMWI GCNNNNNNNGC 1 cut(s) 454
BstNI CCWGG 1 cut(s) 582
BstNSI RCATGY 1 cut(s) 945
BstSCI CCNGG 1 cut(s) 580
BstSFI CTRYAG 1 cut(s) 1031
BstV1I GCAGC 1 cut(s) 316
BstX2I RGATCY 1 cut(s) 506
BstXI CCANNNNNNTGG 1 cut(s) 208
BstYI RGATCY 1 cut(s) 506
BsuRI GGCC 1 cut(s) 212
BtgZI GCGATG 1 cut(s) 142
BtsCI GGATG 3 cut(s) 520, 631, 687
BtsI GCAGTG 1 cut(s) 1028
BtsIMutI CAGTG 1 cut(s) 1028
BveI ACCTGC 1 cut(s) 634
Cac8I GCNNGC 2 cut(s) 210, 290
CaiI CAGNNNCTG 1 cut(s) 376
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 2 cut(s) 161, 212
CseI GACGC 1 cut(s) 373
Csp6I GTAC 3 cut(s) 572, 694, 745
CviAII CATG 2 cut(s) 785, 942
CviQI GTAC 3 cut(s) 572, 694, 745
DdeI CTNAG 2 cut(s) 377, 778
DpnI GATC 5 cut(s) 508, 579, 762, 810, 888
DpnII GATC 5 cut(s) 506, 577, 760, 808, 886
DraI TTTAAA 1 cut(s) 936
DriI GACNNNNNGTC 1 cut(s) 738
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 3 cut(s) 11, 864, 876
Eam1105I GACNNNNNGTC 1 cut(s) 738
EarI CTCTTC 3 cut(s) 11, 864, 876
Eco130I CCWWGG 1 cut(s) 665
EcoRII CCWGG 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 665
ErhI CCWWGG 1 cut(s) 665
Esp3I CGTCTC 1 cut(s) 356
FaeI CATG 2 cut(s) 788, 945
FaqI GGGAC 1 cut(s) 538
FatI CATG 2 cut(s) 784, 941
FauI CCCGC 2 cut(s) 317, 906
FbaI TGATCA 1 cut(s) 760
Fnu4HI GCNGC 4 cut(s) 129, 330, 368, 446
FokI GGATG 3 cut(s) 527, 638, 694
Fsp4HI GCNGC 4 cut(s) 129, 330, 368, 446
GlaI GCGC 1 cut(s) 444
GluI GCNGC 4 cut(s) 129, 330, 368, 446
HaeIII GGCC 1 cut(s) 212
HapII CCGG 3 cut(s) 162, 213, 293
HgaI GACGC 1 cut(s) 373
HhaI GCGC 1 cut(s) 445
Hin1I GRCGYC 1 cut(s) 365
Hin1II CATG 2 cut(s) 788, 945
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 2 cut(s) 61, 565
HindII GTYRAC 2 cut(s) 61, 565
HinfI GANTC 4 cut(s) 136, 281, 345, 958
HpaI GTTAAC 1 cut(s) 61
HpaII CCGG 3 cut(s) 162, 213, 293
HphI GGTGA 2 cut(s) 139, 290
Hpy166II GTNNAC 3 cut(s) 61, 565, 854
Hpy188I TCNGA 8 cut(s) 219, 245, 310, 378, 396, 781, 792, 808
Hpy188III TCNNGA 5 cut(s) 235, 359, 437, 482, 512
Hpy8I GTNNAC 3 cut(s) 61, 565, 854
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 100, 479
HpyCH4III ACNGT 4 cut(s) 145, 151, 278, 598
HpyCH4V TGCA 3 cut(s) 73, 476, 1033
HpyF10VI GCNNNNNNNGC 1 cut(s) 454
HpyF3I CTNAG 2 cut(s) 377, 778
Hsp92I GRCGYC 1 cut(s) 365
Hsp92II CATG 2 cut(s) 788, 945
HspAI GCGC 1 cut(s) 443
Ksp22I TGATCA 1 cut(s) 760
KspAI GTTAAC 1 cut(s) 61
Kzo9I GATC 5 cut(s) 506, 577, 760, 808, 886
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 4 cut(s) 430, 616, 841, 958
MaeIII GTNAC 4 cut(s) 145, 232, 490, 592
MalI GATC 5 cut(s) 508, 579, 762, 810, 888
MboI GATC 5 cut(s) 506, 577, 760, 808, 886
MflI RGATCY 1 cut(s) 506
MhlI GDGCHC 1 cut(s) 1030
MluCI AATT 5 cut(s) 304, 619, 799, 984, 990
MlyI GAGTC 2 cut(s) 290, 967
MseI TTAA 4 cut(s) 60, 896, 935, 989
MspA1I CMGCKG 1 cut(s) 615
MspI CCGG 3 cut(s) 162, 213, 293
MspR9I CCNGG 1 cut(s) 582
MvaI CCWGG 1 cut(s) 582
MwoI GCNNNNNNNGC 1 cut(s) 454
NdeII GATC 5 cut(s) 506, 577, 760, 808, 886
NlaIII CATG 2 cut(s) 788, 945
NlaIV GGNNCC 3 cut(s) 111, 166, 775
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 3 cut(s) 145, 232, 490
NspI RCATGY 1 cut(s) 945
PciI ACATGT 1 cut(s) 941
PcsI WCGNNNNNNNCGW 1 cut(s) 967
PfeI GAWTC 2 cut(s) 136, 345
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 4 cut(s) 130, 331, 369, 447
PleI GAGTC 2 cut(s) 289, 966
PpsI GAGTC 2 cut(s) 289, 966
PscI ACATGT 1 cut(s) 941
PsiI TTATAA 2 cut(s) 341, 797
Psp6I CCWGG 1 cut(s) 580
PspGI CCWGG 1 cut(s) 580
PspN4I GGNNCC 3 cut(s) 111, 166, 775
PsrI GAACNNNNNNTAC 2 cut(s) 577, 609
PstI CTGCAG 1 cut(s) 1035
PstNI CAGNNNCTG 1 cut(s) 376
PsuI RGATCY 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 615
RsaI GTAC 3 cut(s) 573, 695, 746
RsaNI GTAC 3 cut(s) 572, 694, 745
SaqAI TTAA 4 cut(s) 60, 896, 935, 989
SatI GCNGC 4 cut(s) 129, 330, 368, 446
Sau3AI GATC 5 cut(s) 506, 577, 760, 808, 886
ScaI AGTACT 1 cut(s) 573
SchI GAGTC 2 cut(s) 290, 967
ScrFI CCNGG 1 cut(s) 582
SduI GDGCHC 1 cut(s) 1030
SfaNI GCATC 4 cut(s) 430, 616, 841, 958
SfcI CTRYAG 1 cut(s) 1031
SmlI CTYRAG 3 cut(s) 359, 409, 544
SmoI CTYRAG 3 cut(s) 359, 409, 544
Sse9I AATT 5 cut(s) 304, 619, 799, 984, 990
SsiI CCGC 9 cut(s) 29, 129, 324, 352, 368, 446, 703, 913, 947
SspI AATATT 1 cut(s) 1004
StyD4I CCNGG 1 cut(s) 580
StyI CCWWGG 1 cut(s) 665
TaaI ACNGT 4 cut(s) 145, 151, 278, 598
TaqI TCGA 2 cut(s) 192, 961
TasI AATT 5 cut(s) 304, 619, 799, 984, 990
TatI WGTACW 1 cut(s) 571
TauI GCSGC 3 cut(s) 131, 370, 448
TfiI GAWTC 2 cut(s) 136, 345
Tru1I TTAA 4 cut(s) 60, 896, 935, 989
Tru9I TTAA 4 cut(s) 60, 896, 935, 989
TscAI CASTG 1 cut(s) 1035
TseFI GTSAC 3 cut(s) 145, 232, 490
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 3 cut(s) 145, 232, 490
TspDTI ATGAA 1 cut(s) 333
TspRI CASTG 1 cut(s) 1035
XapI RAATTY 2 cut(s) 619, 799
XceI RCATGY 1 cut(s) 945
ZrmI AGTACT 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.