Rh4BG088300

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003646
Physical Location & Seq
Forward (+)
15210549 .. 15223133
12585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG088300.1

Sequence Viewer

Length: 906 bp
ATGACCAAGTATTTCATCGGTAAACCAAAACCAGTTCAGTTCAGTAGTGTCACACTAAAGGAAAGATCTCCAGATGGTGTTGGGACGTTAGTTGATGTGGGTTTGAGTAAGAATGTCGTCGTTGATCAAGTACTTGATCCTGGAGCAAGAGTTACAGTGGCTATGGGAGACAGTTGGAATTTGGATGCTGGTATATCACGGCAGCTTGTATCATCATCCAAGCCAAGGGAAGAAGCAAGGATGTATTGGGGGTACAAAGTGCACTATGCTTCCAATATCACTTCGGTAATGACTGAATGTCCATACGAGGGTGGCTATGATCATTTGATTGGGACCTCAGAGCATGGTCAGATTATCAATTCCTCCGATCTCACTATACCCACTTTCAGGTATCTATTGATTGCGTTTGGTGGATTTGCTGGGTTGGAAGAGAGCATTGAAGAGGATCATAACTTAAAGGAGGAGAAGGAAAGTATAGTCGCTAAGGAAAGAGTTGCATGGGAACAAGAGAGAGATAGACAAGTTGCTGAGGAAAGAGCATATTGGACAGCCAAGATTGCGAGGTTGGAAGCAAAAATTGATGGGAAGGAGGTGCCACTAGAATCACCAGAGTCACCAAAACAATTGACGCCACTTCATGAACATGGTTCAGGCCAAGGGAGCTGTTCCCGACCAGCTGAGAAGATTTTGATTGACATTGTGGAAGGTGATGGCAAAACAGTGAAGAAGAAGTTGGTTTTAGCCGAGGAGAAGATATTTGTTCAAGAGGACCAGCAAGTGGCTACAAATCAACCAATGCAGAAACTAACTTCTATAATTGAAGAGGAGGTTAGAGTCCACATATCTTCGAAAAATTCTGACAATGTAAGCACCTTTCTTGCCTTAATTTATCTCTGTTCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

301

Amino Acids

33.49

Weight (kDa)

5.25

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 17 - 156 4.7e-34 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 592
AccB7I CCANNNNNTGG 1 cut(s) 778
AclWI GGATC 2 cut(s) 131, 453
AcsI RAATTY 2 cut(s) 178, 853
AcyI GRCGYC 1 cut(s) 629
AfaI GTAC 2 cut(s) 132, 254
AfiI CCNNNNNNNGG 4 cut(s) 225, 308, 387, 778
AgsI TTSAA 3 cut(s) 440, 764, 821
AhdI GACNNNNNGTC 1 cut(s) 297
AjnI CCWGG 1 cut(s) 139
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
AluBI AGCT 3 cut(s) 205, 663, 677
AluI AGCT 3 cut(s) 205, 663, 677
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 1 cut(s) 162
Alw44I GTGCAC 1 cut(s) 260
AlwI GGATC 2 cut(s) 131, 453
AoxI GGCC 1 cut(s) 652
ApaLI GTGCAC 1 cut(s) 260
ApeKI GCWGC 1 cut(s) 202
ApoI RAATTY 2 cut(s) 178, 853
AspS9I GGNCC 2 cut(s) 333, 769
AsuHPI GGTGA 3 cut(s) 597, 606, 719
AsuII TTCGAA 1 cut(s) 848
AvaII GGWCC 2 cut(s) 333, 769
BaeGI GKGCMC 1 cut(s) 264
BanI GGYRCC 1 cut(s) 592
Bbv12I GWGCWC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 528
BbvI GCAGC 1 cut(s) 214
BccI CCATC 3 cut(s) 68, 575, 704
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 141
BclI TGATCA 2 cut(s) 124, 319
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 1 cut(s) 599
BglII AGATCT 1 cut(s) 65
BisI GCNGC 1 cut(s) 203
BlsI GCNGC 1 cut(s) 204
BmcAI AGTACT 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 2 cut(s) 333, 769
BmeRI GACNNNNNGTC 1 cut(s) 297
BmgT120I GGNCC 2 cut(s) 333, 769
BmiI GGNNCC 2 cut(s) 334, 594
BmrFI CCNGG 1 cut(s) 141
BmsI GCATC 1 cut(s) 175
BpmI CTGGAG 2 cut(s) 54, 162
Bpu10I CCTNAGC 2 cut(s) 483, 528
Bpu14I TTCGAA 1 cut(s) 848
BsaHI GRCGYC 1 cut(s) 629
BsaJI CCNNGG 3 cut(s) 224, 655, 744
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 308, 387, 778
Bse1I ACTGG 1 cut(s) 32
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 3 cut(s) 224, 655, 744
BseGI GGATG 3 cut(s) 190, 215, 246
BseLI CCNNNNNNNGG 4 cut(s) 225, 308, 387, 778
BseMII CTCAG 3 cut(s) 351, 519, 669
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 3 cut(s) 476, 761, 839
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 1 cut(s) 214
BseYI CCCAGC 1 cut(s) 419
BshFI GGCC 1 cut(s) 654
BshNI GGYRCC 1 cut(s) 592
BsiHKAI GWGCWC 1 cut(s) 264
BslFI GGGAC 2 cut(s) 97, 346
BslI CCNNNNNNNGG 4 cut(s) 225, 308, 387, 778
BsmAI GTCTC 1 cut(s) 162
BsmFI GGGAC 2 cut(s) 97, 346
BsnI GGCC 1 cut(s) 654
Bsp119I TTCGAA 1 cut(s) 848
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 6 cut(s) 65, 124, 136, 319, 367, 445
BspANI GGCC 1 cut(s) 654
BspCNI CTCAG 3 cut(s) 350, 520, 670
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 2 cut(s) 334, 594
BspPI GGATC 2 cut(s) 131, 453
BspT104I TTCGAA 1 cut(s) 848
BspT107I GGYRCC 1 cut(s) 592
BsrI ACTGG 1 cut(s) 32
BssECI CCNNGG 3 cut(s) 224, 655, 744
BssMI GATC 6 cut(s) 65, 124, 136, 319, 367, 445
BssNI GRCGYC 1 cut(s) 629
BssT1I CCWWGG 2 cut(s) 224, 655
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 3 cut(s) 157, 173, 721
Bst6I CTCTTC 3 cut(s) 423, 435, 816
BstACI GRCGYC 1 cut(s) 629
BstBI TTCGAA 1 cut(s) 848
BstDEI CTNAG 4 cut(s) 337, 483, 528, 678
BstF5I GGATG 3 cut(s) 190, 215, 246
BstKTI GATC 6 cut(s) 68, 127, 139, 322, 370, 448
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 6 cut(s) 65, 124, 136, 319, 367, 445
BstMWI GCNNNNNNNGC 2 cut(s) 557, 660
BstNI CCWGG 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 139
BstSLI GKGCMC 1 cut(s) 264
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 1 cut(s) 654
BtsCI GGATG 3 cut(s) 190, 215, 246
BtsIMutI CAGTG 2 cut(s) 162, 726
CciI TCATGA 1 cut(s) 637
Cfr13I GGNCC 2 cut(s) 333, 769
CseI GACGC 1 cut(s) 637
Csp6I GTAC 2 cut(s) 131, 253
CviAII CATG 4 cut(s) 344, 498, 638, 644
CviQI GTAC 2 cut(s) 131, 253
DdeI CTNAG 4 cut(s) 337, 483, 528, 678
DpnI GATC 6 cut(s) 67, 126, 138, 321, 369, 447
DpnII GATC 6 cut(s) 65, 124, 136, 319, 367, 445
DriI GACNNNNNGTC 1 cut(s) 297
Eam1104I CTCTTC 3 cut(s) 423, 435, 816
Eam1105I GACNNNNNGTC 1 cut(s) 297
EarI CTCTTC 3 cut(s) 423, 435, 816
Eco130I CCWWGG 2 cut(s) 224, 655
Eco47I GGWCC 2 cut(s) 333, 769
EcoO109I RGGNCCY 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 2 cut(s) 224, 655
ErhI CCWWGG 2 cut(s) 224, 655
FaeI CATG 4 cut(s) 347, 501, 641, 647
FaqI GGGAC 2 cut(s) 97, 346
FatI CATG 4 cut(s) 343, 497, 637, 643
FbaI TGATCA 2 cut(s) 124, 319
Fnu4HI GCNGC 1 cut(s) 203
FokI GGATG 3 cut(s) 197, 202, 253
Fsp4HI GCNGC 1 cut(s) 203
FspBI CTAG 1 cut(s) 599
GluI GCNGC 1 cut(s) 203
GsaI CCCAGC 1 cut(s) 423
GsuI CTGGAG 2 cut(s) 54, 162
HaeIII GGCC 1 cut(s) 654
HgaI GACGC 1 cut(s) 637
Hin1I GRCGYC 1 cut(s) 629
Hin1II CATG 4 cut(s) 347, 501, 641, 647
HinfI GANTC 3 cut(s) 602, 611, 834
HphI GGTGA 3 cut(s) 597, 606, 719
Hpy166II GTNNAC 3 cut(s) 23, 262, 838
Hpy188I TCNGA 4 cut(s) 340, 351, 367, 859
Hpy188III TCNNGA 4 cut(s) 71, 638, 669, 764
Hpy8I GTNNAC 3 cut(s) 23, 262, 838
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 460, 580, 698
HpyCH4III ACNGT 3 cut(s) 157, 173, 721
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 3 cut(s) 262, 497, 799
HpyF10VI GCNNNNNNNGC 2 cut(s) 557, 660
HpyF3I CTNAG 4 cut(s) 337, 483, 528, 678
HpySE526I ACGT 1 cut(s) 86
Hsp92I GRCGYC 1 cut(s) 629
Hsp92II CATG 4 cut(s) 347, 501, 641, 647
Ksp22I TGATCA 2 cut(s) 124, 319
Kzo9I GATC 6 cut(s) 65, 124, 136, 319, 367, 445
LmnI GCTCC 2 cut(s) 143, 660
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 1 cut(s) 175
MaeI CTAG 1 cut(s) 599
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 3 cut(s) 49, 151, 612
MalI GATC 6 cut(s) 67, 126, 138, 321, 369, 447
MboI GATC 6 cut(s) 65, 124, 136, 319, 367, 445
MboII GAAGA 9 cut(s) 242, 440, 452, 694, 736, 739, 763, 833, 837
MfeI CAATTG 1 cut(s) 623
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 7 cut(s) 178, 358, 576, 623, 816, 853, 885
MlyI GAGTC 2 cut(s) 620, 843
MmeI TCCRAC 3 cut(s) 155, 405, 546
MseI TTAA 3 cut(s) 455, 884, 904
MslI CAYNNNNRTG 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 677
MspR9I CCNGG 1 cut(s) 141
MunI CAATTG 1 cut(s) 623
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 557, 660
NdeII GATC 6 cut(s) 65, 124, 136, 319, 367, 445
NlaIII CATG 4 cut(s) 347, 501, 641, 647
NlaIV GGNNCC 2 cut(s) 334, 594
NmeAIII GCCGAG 1 cut(s) 769
NmuCI GTSAC 2 cut(s) 49, 612
NspV TTCGAA 1 cut(s) 848
PagI TCATGA 1 cut(s) 637
PfeI GAWTC 1 cut(s) 602
PflMI CCANNNNNTGG 1 cut(s) 778
PfoI TCCNGGA 1 cut(s) 139
PkrI GCNGC 1 cut(s) 204
PleI GAGTC 2 cut(s) 619, 842
PpsI GAGTC 2 cut(s) 619, 842
PpuMI RGGWCCY 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 139
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 2 cut(s) 334, 594
PspPI GGNCC 2 cut(s) 333, 769
PspPPI RGGWCCY 1 cut(s) 333
PsuI RGATCY 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 2 cut(s) 132, 254
RsaNI GTAC 2 cut(s) 131, 253
RseI CAYNNNNRTG 1 cut(s) 642
SaqAI TTAA 3 cut(s) 455, 884, 904
SatI GCNGC 1 cut(s) 203
Sau3AI GATC 6 cut(s) 65, 124, 136, 319, 367, 445
Sau96I GGNCC 2 cut(s) 333, 769
ScaI AGTACT 1 cut(s) 132
SchI GAGTC 2 cut(s) 620, 843
ScrFI CCNGG 1 cut(s) 141
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 1 cut(s) 175
SfuI TTCGAA 1 cut(s) 848
SinI GGWCC 2 cut(s) 333, 769
SmiMI CAYNNNNRTG 1 cut(s) 642
Sse9I AATT 7 cut(s) 178, 358, 576, 623, 816, 853, 885
SspMI CTAG 1 cut(s) 599
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 2 cut(s) 224, 655
TaaI ACNGT 3 cut(s) 157, 173, 721
TaiI ACGT 1 cut(s) 89
TaqI TCGA 1 cut(s) 848
TasI AATT 7 cut(s) 178, 358, 576, 623, 816, 853, 885
TatI WGTACW 1 cut(s) 130
TfiI GAWTC 1 cut(s) 602
Tru1I TTAA 3 cut(s) 455, 884, 904
Tru9I TTAA 3 cut(s) 455, 884, 904
TscAI CASTG 2 cut(s) 162, 726
TseFI GTSAC 2 cut(s) 49, 612
TseI GCWGC 1 cut(s) 202
Tsp45I GTSAC 2 cut(s) 49, 612
TspDTI ATGAA 3 cut(s) 4, 626, 654
TspRI CASTG 2 cut(s) 162, 726
Van91I CCANNNNNTGG 1 cut(s) 778
VneI GTGCAC 1 cut(s) 260
VpaK11BI GGWCC 2 cut(s) 333, 769
XapI RAATTY 2 cut(s) 178, 853
XspI CTAG 1 cut(s) 599
ZrmI AGTACT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.