Rw4G017680

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003646
Physical Location & Seq
Reverse (-)
39742966 .. 39746758
3793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G017680.1

Sequence Viewer

Length: 1041 bp
ATGGGTAAAAAGAAGAAGAGAACCGAAGCGGAAGAAGAAATCAAAGCCGACGACATTGTTAACGGCGACTTGCATAACAAAACCAACAAGAAGAAGAAGAAGAATAAACCCAAAACCGACACAGCCGCCGTGATTCCCACCGTCACCGTAGCCATCGCCGGTTCCATCATTCACAACGCTCAATCCCTCGAACTCGCCACTCGGCTGGCCGGTCAGATAGCTCGTGCCGTCACGATTTTCCGAGTTGATGAGGTGGTGGTATTTGACAACAACGGTGAGTCTGGCTCGCCGGAAGCTAATAATTCCGATGAGAATGAAAGCGGGGCTGCTTTTCTTATAAGAATCTTGCGGTATCTTGAGACGCCGCAGTATCTGAGGAAAGCTCTATTTCCGAAACATAATAGCTTGAGATTTGTGGGCTTGTTGCCTCCTCTTGATGCGCCGCACCATCTGCGTAGACACGAATGGGGTGCATTTCGGGAAGGTGTCACGCTAAAGGAAAGATCTCCGGATGGTGTTGGGACATTAGTTGATGTGGGCTTGAGTAAGAATGTTGTCGTTGACCACGTACTTGATCCTGGAACAAGAGTTACTGTGGCTATGGGAGACAGCTGGAATTTGGATGCTGATATATCACGGCAGGTTGTATCATCATCCAAGCCAAGGGAAGAAGCAGGGATGTATTGGGGGTACAAAGTGCGGTATGCTTCCAATATCACTTCGGTAATGACTGAATGTCCGTACGAGGGTGGTTATGATCATTTGATTGGAACCTCAGAGCATGGTCAGATTATAAATTCCTCTGATCTCAGTATACCTACTTTCAGGCATCTATTGATTGCTTTCGGTGGACTTGCTGGATTGGAAGAGAGCATTGAAGAGGATCATAACTTAAAGGCAAAGAATGTGCGGGAGGTGTTTAATATATATTTAAACACATGTCCGCATCAGGGGAGTCGAACAATACGAACAGAGGAAGCAATTTTAATTTCACTTCAATATTTCCAAGAACCAATCAACAGAGCACTGCAGAGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

346

Amino Acids

38.43

Weight (kDa)

6.65

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 47 - 334 1.8e-109 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 338, 794
Acc36I ACCTGC 1 cut(s) 631
AccI GTMKAC 2 cut(s) 457, 814
AccIII TCCGGA 1 cut(s) 508
AciI CCGC 9 cut(s) 29, 126, 321, 349, 365, 443, 700, 910, 944
AclWI GGATC 2 cut(s) 569, 891
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 2 cut(s) 616, 796
AcyI GRCGYC 1 cut(s) 362
AfaI GTAC 3 cut(s) 570, 692, 743
AfiI CCNNNNNNNGG 3 cut(s) 663, 746, 950
AflIII ACRYGT 1 cut(s) 938
AgsI TTSAA 2 cut(s) 878, 998
AhdI GACNNNNNGTC 1 cut(s) 735
AjnI CCWGG 1 cut(s) 577
AluBI AGCT 5 cut(s) 221, 296, 383, 405, 612
AluI AGCT 5 cut(s) 221, 296, 383, 405, 612
Alw21I GWGCWC 1 cut(s) 1027
Alw26I GTCTC 2 cut(s) 353, 600
AlwI GGATC 2 cut(s) 569, 891
AlwNI CAGNNNCTG 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 508
AoxI GGCC 1 cut(s) 207
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 2 cut(s) 616, 796
AspLEI GCGC 1 cut(s) 442
AsuHPI GGTGA 2 cut(s) 136, 287
BauI CACGAG 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 1027
BbvI GCAGC 1 cut(s) 313
BccI CCATC 4 cut(s) 161, 173, 456, 506
BceAI ACGGC 4 cut(s) 79, 113, 212, 653
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 1 cut(s) 579
BclI TGATCA 1 cut(s) 757
BcoDI GTCTC 2 cut(s) 353, 600
BfmI CTRYAG 1 cut(s) 1028
BfuAI ACCTGC 1 cut(s) 631
BglII AGATCT 1 cut(s) 503
BisI GCNGC 4 cut(s) 126, 327, 365, 443
BlsI GCNGC 4 cut(s) 127, 328, 366, 444
Bme1390I CCNGG 1 cut(s) 579
BmeRI GACNNNNNGTC 1 cut(s) 735
BmiI GGNNCC 2 cut(s) 163, 772
BmrFI CCNGG 1 cut(s) 579
BmsI GCATC 4 cut(s) 427, 613, 838, 955
BplI GAGNNNNNCTC 4 cut(s) 269, 301, 367, 399
BpuEI CTTGAG 3 cut(s) 377, 427, 562
BsaAI YACGTR 1 cut(s) 568
BsaHI GRCGYC 1 cut(s) 362
BsaJI CCNNGG 1 cut(s) 662
BsaWI WCCGGW 1 cut(s) 508
Bsc4I CCNNNNNNNGG 3 cut(s) 663, 746, 950
Bse118I RCCGGY 2 cut(s) 158, 209
BseAI TCCGGA 1 cut(s) 508
BseBI CCWGG 1 cut(s) 579
BseDI CCNNGG 1 cut(s) 662
BseGI GGATG 4 cut(s) 517, 628, 653, 684
BseLI CCNNNNNNNGG 3 cut(s) 663, 746, 950
BseMII CTCAG 3 cut(s) 365, 789, 823
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 1 cut(s) 313
BshFI GGCC 1 cut(s) 209
BsiHKAI GWGCWC 1 cut(s) 1027
BsiSI CCGG 4 cut(s) 159, 210, 290, 509
BsiWI CGTACG 1 cut(s) 741
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 3 cut(s) 663, 746, 950
BsmAI GTCTC 2 cut(s) 353, 600
BsmBI CGTCTC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 1027
Bsp13I TCCGGA 1 cut(s) 508
Bsp143I GATC 5 cut(s) 503, 574, 757, 805, 883
BspACI CCGC 9 cut(s) 29, 126, 321, 349, 365, 443, 700, 910, 944
BspANI GGCC 1 cut(s) 209
BspCNI CTCAG 3 cut(s) 366, 788, 822
BspEI TCCGGA 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 163, 772
BspMAI CTGCAG 1 cut(s) 1032
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 2 cut(s) 569, 891
BsrFI RCCGGY 2 cut(s) 158, 209
BssAI RCCGGY 2 cut(s) 158, 209
BssECI CCNNGG 1 cut(s) 662
BssMI GATC 5 cut(s) 503, 574, 757, 805, 883
BssNAI GTATAC 1 cut(s) 815
BssNI GRCGYC 1 cut(s) 362
BssSI CACGAG 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 662
Bst1107I GTATAC 1 cut(s) 815
Bst2BI CACGAG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 4 cut(s) 142, 148, 275, 595
Bst6I CTCTTC 3 cut(s) 11, 861, 873
BstACI GRCGYC 1 cut(s) 362
BstAPI GCANNNNNTGC 1 cut(s) 451
BstBAI YACGTR 1 cut(s) 568
BstC8I GCNNGC 2 cut(s) 207, 287
BstDEI CTNAG 3 cut(s) 374, 775, 809
BstF5I GGATG 4 cut(s) 517, 628, 653, 684
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 5 cut(s) 506, 577, 760, 808, 886
BstMAI GTCTC 2 cut(s) 353, 600
BstMBI GATC 5 cut(s) 503, 574, 757, 805, 883
BstMWI GCNNNNNNNGC 1 cut(s) 451
BstNI CCWGG 1 cut(s) 579
BstNSI RCATGY 1 cut(s) 942
BstSCI CCNGG 1 cut(s) 577
BstSFI CTRYAG 1 cut(s) 1028
BstV1I GCAGC 1 cut(s) 313
BstX2I RGATCY 1 cut(s) 503
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 1 cut(s) 503
BstZ17I GTATAC 1 cut(s) 815
BsuRI GGCC 1 cut(s) 209
BtgZI GCGATG 1 cut(s) 139
BtsCI GGATG 4 cut(s) 517, 628, 653, 684
BtsI GCAGTG 1 cut(s) 1025
BtsIMutI CAGTG 1 cut(s) 1025
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 2 cut(s) 207, 287
CaiI CAGNNNCTG 1 cut(s) 373
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 2 cut(s) 158, 209
CseI GACGC 1 cut(s) 370
Csp6I GTAC 3 cut(s) 569, 691, 742
CviAII CATG 2 cut(s) 782, 939
CviQI GTAC 3 cut(s) 569, 691, 742
DdeI CTNAG 3 cut(s) 374, 775, 809
DpnI GATC 5 cut(s) 505, 576, 759, 807, 885
DpnII GATC 5 cut(s) 503, 574, 757, 805, 883
DraI TTTAAA 1 cut(s) 933
DriI GACNNNNNGTC 1 cut(s) 735
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 3 cut(s) 11, 861, 873
Eam1105I GACNNNNNGTC 1 cut(s) 735
EarI CTCTTC 3 cut(s) 11, 861, 873
Eco130I CCWWGG 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 577
EcoT14I CCWWGG 1 cut(s) 662
ErhI CCWWGG 1 cut(s) 662
Esp3I CGTCTC 1 cut(s) 353
FaeI CATG 2 cut(s) 785, 942
FaqI GGGAC 1 cut(s) 535
FatI CATG 2 cut(s) 781, 938
FauI CCCGC 2 cut(s) 314, 903
FbaI TGATCA 1 cut(s) 757
FblI GTMKAC 2 cut(s) 457, 814
Fnu4HI GCNGC 4 cut(s) 126, 327, 365, 443
FokI GGATG 4 cut(s) 524, 635, 640, 691
Fsp4HI GCNGC 4 cut(s) 126, 327, 365, 443
GlaI GCGC 1 cut(s) 441
GluI GCNGC 4 cut(s) 126, 327, 365, 443
HaeIII GGCC 1 cut(s) 209
HapII CCGG 4 cut(s) 159, 210, 290, 509
HgaI GACGC 1 cut(s) 370
HhaI GCGC 1 cut(s) 442
Hin1I GRCGYC 1 cut(s) 362
Hin1II CATG 2 cut(s) 785, 942
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HincII GTYRAC 2 cut(s) 61, 562
HindII GTYRAC 2 cut(s) 61, 562
HinfI GANTC 4 cut(s) 133, 278, 342, 955
HpaI GTTAAC 1 cut(s) 61
HpaII CCGG 4 cut(s) 159, 210, 290, 509
HphI GGTGA 2 cut(s) 136, 287
Hpy166II GTNNAC 5 cut(s) 61, 458, 562, 815, 851
Hpy188I TCNGA 8 cut(s) 216, 242, 307, 375, 393, 778, 789, 805
Hpy188III TCNNGA 5 cut(s) 232, 356, 434, 479, 509
Hpy8I GTNNAC 5 cut(s) 61, 458, 562, 815, 851
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 1 cut(s) 476
HpyCH4III ACNGT 4 cut(s) 142, 148, 275, 595
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 3 cut(s) 73, 473, 1030
HpyF10VI GCNNNNNNNGC 1 cut(s) 451
HpyF3I CTNAG 3 cut(s) 374, 775, 809
HpySE526I ACGT 1 cut(s) 567
Hsp92I GRCGYC 1 cut(s) 362
Hsp92II CATG 2 cut(s) 785, 942
HspAI GCGC 1 cut(s) 440
Kpn2I TCCGGA 1 cut(s) 508
Ksp22I TGATCA 1 cut(s) 757
KspAI GTTAAC 1 cut(s) 61
Kzo9I GATC 5 cut(s) 503, 574, 757, 805, 883
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 4 cut(s) 427, 613, 838, 955
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 4 cut(s) 142, 229, 487, 589
MalI GATC 5 cut(s) 505, 576, 759, 807, 885
MboI GATC 5 cut(s) 503, 574, 757, 805, 883
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 1 cut(s) 1027
MluCI AATT 5 cut(s) 301, 616, 796, 981, 987
MlyI GAGTC 2 cut(s) 287, 964
MroI TCCGGA 1 cut(s) 508
MseI TTAA 5 cut(s) 60, 893, 921, 932, 986
MspA1I CMGCKG 1 cut(s) 612
MspI CCGG 4 cut(s) 159, 210, 290, 509
MspR9I CCNGG 1 cut(s) 579
MvaI CCWGG 1 cut(s) 579
MwoI GCNNNNNNNGC 1 cut(s) 451
NdeII GATC 5 cut(s) 503, 574, 757, 805, 883
NlaIII CATG 2 cut(s) 785, 942
NlaIV GGNNCC 2 cut(s) 163, 772
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 3 cut(s) 142, 229, 487
NspI RCATGY 1 cut(s) 942
PciI ACATGT 1 cut(s) 938
PcsI WCGNNNNNNNCGW 2 cut(s) 564, 964
PfeI GAWTC 2 cut(s) 133, 342
Pfl23II CGTACG 1 cut(s) 741
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 4 cut(s) 127, 328, 366, 444
PleI GAGTC 2 cut(s) 286, 963
PpsI GAGTC 2 cut(s) 286, 963
Ppu21I YACGTR 1 cut(s) 568
PscI ACATGT 1 cut(s) 938
PsiI TTATAA 2 cut(s) 338, 794
Psp6I CCWGG 1 cut(s) 577
PspGI CCWGG 1 cut(s) 577
PspLI CGTACG 1 cut(s) 741
PspN4I GGNNCC 2 cut(s) 163, 772
PsrI GAACNNNNNNTAC 2 cut(s) 574, 606
PstI CTGCAG 1 cut(s) 1032
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 612
RsaI GTAC 3 cut(s) 570, 692, 743
RsaNI GTAC 3 cut(s) 569, 691, 742
SaqAI TTAA 5 cut(s) 60, 893, 921, 932, 986
SatI GCNGC 4 cut(s) 126, 327, 365, 443
Sau3AI GATC 5 cut(s) 503, 574, 757, 805, 883
SchI GAGTC 2 cut(s) 287, 964
ScrFI CCNGG 1 cut(s) 579
SduI GDGCHC 1 cut(s) 1027
SfaNI GCATC 4 cut(s) 427, 613, 838, 955
SfcI CTRYAG 1 cut(s) 1028
SmlI CTYRAG 3 cut(s) 356, 406, 541
SmoI CTYRAG 3 cut(s) 356, 406, 541
Sse9I AATT 5 cut(s) 301, 616, 796, 981, 987
SsiI CCGC 9 cut(s) 29, 126, 321, 349, 365, 443, 700, 910, 944
SspI AATATT 1 cut(s) 1001
StyD4I CCNGG 1 cut(s) 577
StyI CCWWGG 1 cut(s) 662
TaaI ACNGT 4 cut(s) 142, 148, 275, 595
TaiI ACGT 1 cut(s) 570
TaqI TCGA 2 cut(s) 189, 958
TasI AATT 5 cut(s) 301, 616, 796, 981, 987
TauI GCSGC 3 cut(s) 128, 367, 445
TfiI GAWTC 2 cut(s) 133, 342
Tru1I TTAA 5 cut(s) 60, 893, 921, 932, 986
Tru9I TTAA 5 cut(s) 60, 893, 921, 932, 986
TscAI CASTG 1 cut(s) 1032
TseFI GTSAC 3 cut(s) 142, 229, 487
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 3 cut(s) 142, 229, 487
TspDTI ATGAA 1 cut(s) 330
TspGWI ACGGA 1 cut(s) 729
TspRI CASTG 1 cut(s) 1032
XapI RAATTY 2 cut(s) 616, 796
XceI RCATGY 1 cut(s) 942
XmiI GTMKAC 2 cut(s) 457, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.