AT5G48655

Zinc finger (C3HC4-type RING finger) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001134
Physical Location & Seq
Reverse (-)
19730380 .. 19733156
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G48655.5

Sequence Viewer

Length: 612 bp
ATGAACACGTCAGAGGTGAGAGTACCTCGAGGAAATCGACGGAGGAAAGCTGTGATTGATCTGAATGCGGTACCTGTTGATCAAGAAGGGACCTCTGCTTCTGTTAGAACTCTTACGGTGCCTATTACACCGTCTCAGCCTGCTCCTACGATGATTGATGTCGATGCTATTGAGGATGATGTTATTGAATCATCCGCTAGTGCTTTTGCTGAAGCTAAAAGCAAATCAAGAAATGCACGTCGGAGACCTTTGATGGTTGATGTAGAGTCAGGAGGTACGACTAGATTCCCTGCCAACATAAGCAACAAACGCAGAAGGATTCCTTCTAGTGAATCTGTCATCGACTGTGAGCATGCCTCTGTAAATGATGAAGTCAACATGTCTTCGAGAGTGTCTAGATCAAAGGCTCCAGCTCCTCCACCAGAAGAGCCAAAGTTTACATGTCCAATCTGCATGTGTCCCTTTACGGAGGAGATGTCAACCAAGTGCGGTCACATCTTCTGCAAGGGATGTATAAAGATGGCAATATCTCGCCAGGGCAAATGCCCTACTTGTAGGAAAAAGGTTACTGCAAAAGAGCTGATTCGAGTTTTCCTTCCAACCACTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_2 zf-RING_2

Protein Analysis

203

Amino Acids

22.3

Weight (kDa)

9.39

Isoelectric Point (pI)

84.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 147 - 186 6.8e-09 Ring finger domain
zf-C3HC4_3 PF13920 147 - 190 3.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 148 - 183 1.3e-07 RING-type zinc-finger
zf-C3HC4_2 PF13923 148 - 185 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 148 - 185 3.9e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 27
Acc65I GGTACC 1 cut(s) 70
AccB1I GGYRCC 2 cut(s) 70, 118
AciI CCGC 3 cut(s) 68, 195, 489
AcuI CTGAAG 1 cut(s) 231
AfaI GTAC 3 cut(s) 24, 72, 277
AflIII ACRYGT 3 cut(s) 6, 378, 440
AgsI TTSAA 1 cut(s) 188
AjiI CACGTC 2 cut(s) 9, 239
AjnI CCWGG 1 cut(s) 534
AluBI AGCT 4 cut(s) 50, 215, 413, 580
AluI AGCT 4 cut(s) 50, 215, 413, 580
Alw26I GTCTC 2 cut(s) 138, 238
Ama87I CYCGRG 1 cut(s) 27
Asp718I GGTACC 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 90
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 1 cut(s) 90
BanI GGYRCC 2 cut(s) 70, 118
BbsI GAAGAC 1 cut(s) 375
BccI CCATC 2 cut(s) 247, 514
BciT130I CCWGG 1 cut(s) 536
BclI TGATCA 1 cut(s) 79
BcoDI GTCTC 2 cut(s) 138, 238
BfaI CTAG 5 cut(s) 198, 282, 327, 396, 606
Bme1390I CCNGG 1 cut(s) 536
Bme18I GGWCC 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 27
BmgBI CACGTC 2 cut(s) 9, 239
BmgT120I GGNCC 1 cut(s) 90
BmiI GGNNCC 4 cut(s) 72, 91, 120, 408
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 4 cut(s) 10, 42, 341, 373
BpmI CTGGAG 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 238
BsaJI CCNNGG 1 cut(s) 535
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 1 cut(s) 535
BseGI GGATG 3 cut(s) 181, 191, 515
BseMII CTCAG 1 cut(s) 149
BseRI GAGGAG 2 cut(s) 405, 485
BshNI GGYRCC 2 cut(s) 70, 118
BsiHKCI CYCGRG 1 cut(s) 27
BslFI GGGAC 2 cut(s) 103, 444
BsmAI GTCTC 2 cut(s) 138, 238
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 2 cut(s) 103, 444
BsmI GAATGC 1 cut(s) 70
Bso31I GGTCTC 1 cut(s) 238
BsoBI CYCGRG 1 cut(s) 27
Bsp143I GATC 3 cut(s) 58, 79, 398
BspACI CCGC 3 cut(s) 68, 195, 489
BspCNI CTCAG 1 cut(s) 148
BspLI GGNNCC 4 cut(s) 72, 91, 120, 408
BspQI GCTCTTC 1 cut(s) 420
BspT107I GGYRCC 2 cut(s) 70, 118
BspTNI GGTCTC 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 535
BssMI GATC 3 cut(s) 58, 79, 398
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 3 cut(s) 118, 132, 347
Bst6I CTCTTC 1 cut(s) 420
BstC8I GCNNGC 2 cut(s) 141, 354
BstDEI CTNAG 1 cut(s) 135
BstF5I GGATG 3 cut(s) 181, 191, 515
BstKTI GATC 3 cut(s) 61, 82, 401
BstMAI GTCTC 2 cut(s) 138, 238
BstMBI GATC 3 cut(s) 58, 79, 398
BstMWI GCNNNNNNNGC 1 cut(s) 309
BstNI CCWGG 1 cut(s) 536
BstNSI RCATGY 4 cut(s) 356, 382, 444, 457
BstSCI CCNGG 1 cut(s) 534
BstV2I GAAGAC 1 cut(s) 375
BtrI CACGTC 2 cut(s) 9, 239
BtsCI GGATG 3 cut(s) 181, 191, 515
Cac8I GCNNGC 2 cut(s) 141, 354
Cfr13I GGNCC 1 cut(s) 90
Csp6I GTAC 3 cut(s) 23, 71, 276
CviAII CATG 4 cut(s) 353, 379, 441, 454
CviJI RGCY 7 cut(s) 50, 139, 215, 407, 413, 430, 580
CviKI_1 RGCY 7 cut(s) 50, 139, 215, 407, 413, 430, 580
CviQI GTAC 3 cut(s) 23, 71, 276
DdeI CTNAG 1 cut(s) 135
DpnI GATC 3 cut(s) 60, 81, 400
DpnII GATC 3 cut(s) 58, 79, 398
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 534
Esp3I CGTCTC 1 cut(s) 138
FaeI CATG 4 cut(s) 356, 382, 444, 457
FaiI YATR 6 cut(s) 299, 354, 380, 442, 455, 515
FalI AAGNNNNNCTT 2 cut(s) 307, 339
FaqI GGGAC 2 cut(s) 103, 444
FatI CATG 4 cut(s) 352, 378, 440, 453
FbaI TGATCA 1 cut(s) 79
FokI GGATG 3 cut(s) 178, 188, 522
FspBI CTAG 5 cut(s) 198, 282, 327, 396, 606
GsuI CTGGAG 1 cut(s) 393
Hin1II CATG 4 cut(s) 356, 382, 444, 457
HincII GTYRAC 2 cut(s) 376, 480
HindII GTYRAC 2 cut(s) 376, 480
HinfI GANTC 6 cut(s) 188, 266, 285, 319, 332, 583
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 3 cut(s) 376, 438, 480
Hpy188I TCNGA 3 cut(s) 13, 63, 243
Hpy188III TCNNGA 5 cut(s) 83, 228, 270, 387, 396
Hpy8I GTNNAC 3 cut(s) 376, 438, 480
Hpy99I CGWCG 2 cut(s) 42, 243
HpyAV CCTTC 4 cut(s) 80, 309, 333, 605
HpyCH4III ACNGT 3 cut(s) 118, 132, 347
HpyCH4IV ACGT 2 cut(s) 8, 238
HpyCH4V TGCA 4 cut(s) 236, 453, 504, 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 309
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 2 cut(s) 8, 238
Hsp92II CATG 4 cut(s) 356, 382, 444, 457
KpnI GGTACC 1 cut(s) 74
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 3 cut(s) 58, 79, 398
LguI GCTCTTC 1 cut(s) 420
LmnI GCTCC 3 cut(s) 148, 412, 418
LpnPI CCDG 8 cut(s) 87, 153, 255, 303, 423, 435, 521, 548
LweI GCATC 1 cut(s) 154
MaeI CTAG 5 cut(s) 198, 282, 327, 396, 606
MaeII ACGT 2 cut(s) 8, 238
MaeIII GTNAC 2 cut(s) 491, 565
MalI GATC 3 cut(s) 60, 81, 400
MboI GATC 3 cut(s) 58, 79, 398
MboII GAAGA 3 cut(s) 375, 437, 490
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 536
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 1 cut(s) 309
NdeII GATC 3 cut(s) 58, 79, 398
NlaIII CATG 4 cut(s) 356, 382, 444, 457
NlaIV GGNNCC 4 cut(s) 72, 91, 120, 408
NmuCI GTSAC 1 cut(s) 491
NspI RCATGY 4 cut(s) 356, 382, 444, 457
PaeI GCATGC 1 cut(s) 356
PaeR7I CTCGAG 1 cut(s) 27
PciI ACATGT 2 cut(s) 378, 440
PciSI GCTCTTC 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 34
PctI GAATGC 1 cut(s) 70
PfeI GAWTC 5 cut(s) 188, 285, 319, 332, 583
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PpuMI RGGWCCY 1 cut(s) 90
PscI ACATGT 2 cut(s) 378, 440
Psp5II RGGWCCY 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 4 cut(s) 72, 91, 120, 408
PspPI GGNCC 1 cut(s) 90
PspPPI RGGWCCY 1 cut(s) 90
PspXI VCTCGAGB 1 cut(s) 27
RsaI GTAC 3 cut(s) 24, 72, 277
RsaNI GTAC 3 cut(s) 23, 71, 276
SapI GCTCTTC 1 cut(s) 420
Sau3AI GATC 3 cut(s) 58, 79, 398
Sau96I GGNCC 1 cut(s) 90
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 1 cut(s) 536
SfaNI GCATC 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 90
SlaI CTCGAG 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
SphI GCATGC 1 cut(s) 356
SsiI CCGC 3 cut(s) 68, 195, 489
SspMI CTAG 5 cut(s) 198, 282, 327, 396, 606
StyD4I CCNGG 1 cut(s) 534
TaaI ACNGT 3 cut(s) 118, 132, 347
TaiI ACGT 2 cut(s) 11, 241
TaqI TCGA 6 cut(s) 28, 37, 162, 342, 386, 586
TfiI GAWTC 5 cut(s) 188, 285, 319, 332, 583
TseFI GTSAC 1 cut(s) 491
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 2 cut(s) 17, 384
TspGWI ACGGA 2 cut(s) 55, 482
VpaK11BI GGWCC 1 cut(s) 90
XbaI TCTAGA 1 cut(s) 395
XceI RCATGY 4 cut(s) 356, 382, 444, 457
XhoI CTCGAG 1 cut(s) 27
XspI CTAG 5 cut(s) 198, 282, 327, 396, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.