Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001134

Physical Location & Seq

Genomic Coordinates Forward (+)

1586252 .. 1588923

Gene Structure 2672 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1014000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 639 bp

ATGAGTACACAAGGATCTAGTGCATATCGACAATGGAATCTAAGAGATGCTCAAAGGGGAGGCATGAGAGATTTGGACCTCAATTATCCGCCTCCTCGCGAGAACATGGCTCCGACTGGTCCATTGCGAATACAAGCTCAAGGAAGTGGACATTCTCAAGGACAAGCAACAACCGTTGCGGATCTTGTTGATGATGATGATGTTGTTGTAATATCACCAAGGAAGTTTGAAGAAGCGAGGAATAATGCTCGAAGAAATCATTCTCGAAGAAACGGTAGAGTCATAGAAGGTTTTTCTGAAGAATTGACAAATTGGTGCCAACTTGGAGATCTTGGAGCTTTCAACTGGGAGCTTTCCTTCAATTTCGATTTCATCAACAAAGCAGAGGATACTATACATAGCGTTTCAGTGACTCCAGTAGCGCCCCAGACCCAGAGTGTGCCCCCGCCAGAGGCACCTACTTTCACTTGTGCAATATGCATGGGCCAGTTGATTGAAGAAACGTCAACAAAGTGTGGTCACATCTTCTGCAAGGAGTGCATTGACACAGCCATAGCTACTCAACACAAATGCCCTACCTGCAGGCATAAACTTAGAAAGCGAGACACCATCAGGGTCTACCTTCCAAGCAGCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_2

Protein Analysis

213

Amino Acids

23.68

Weight (kDa)

6.59

Isoelectric Point (pI)

57.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	156 - 195	1.5e-09	Ring finger domain
zf-C3HC4_3	PF13920	156 - 200	3.7e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	157 - 194	3.4e-10	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	157 - 194	1.2e-08	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0001134)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07200 AT3G07200 AT3G07200 AT5G48655 AT5G48655 AT5G48655 AT5G48655 AT5G48655 AT5G48655
fragaria_vesca	FvH4_2g18620 FvH4_2g18620 FvH4_2g18620 FvH4_2g18620 FvH4_4g13470 FvH4_4g13470 FvH4_4g13470 FvH4_4g13470 FvH4_4g13481
malus_domestica	MD04G1013800.v1.1 MD04G1014000.v1.1 MD05G1172100.v1.1
prunus_persica	Prupe.1G160800_v2.0.a1 Prupe.1G161000_v2.0.a1 Prupe.1G161000_v2.0.a1 Prupe.8G199600_v2.0.a1 Prupe.8G199600_v2.0.a1
pyrus_communis	pycom05g15880
rosa_chinensis	RchiOBHm_Chr4g0414081 RchiOBHm_Chr4g0414101 RchiOBHm_Chr6g0284181
rosa_laevigata	RLG00000008177 RLG00000008179 RLG00000012760
rosa_multiflora	Rmu_sc0003264.1_g000011 Rmu_sc0006428.1_g000005
rosa_roxburghii	Rroxscaffold_5G00358010 Rroxscaffold_5G00358030 Rroxscaffold_7G00184810
rosa_rugosa	Rorug04G0125200 Rorug04G0125400 Rorug04G0125500 Rorug06G0161700 Rorug06G0161700
rosa_samantha	Rh4AG183900 Rh4AG184100 Rh4BG183400 Rh4BG183600 Rh4CG195800 Rh4CG196000 Rh4DG179300 Rh4DG179500 Rh6AG274500 Rh6BG274900 Rh6CG276700 Rh6DG269300
rosa_wichuraiana	Rw4G015650 Rw4G015670 Rw6G023610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	587
AccB1I	GGYRCC	2 cut(s)	315, 454
AccI	GTMKAC	1 cut(s)	618
AccII	CGCG	1 cut(s)	99
AciI	CCGC	3 cut(s)	89, 179, 446
AclWI	GGATC	2 cut(s)	22, 189
AcuI	CTGAAG	1 cut(s)	318
AfaI	GTAC	1 cut(s)	7
AfiI	CCNNNNNNNGG	1 cut(s)	451
AgsI	TTSAA	4 cut(s)	230, 343, 361, 497
AluBI	AGCT	4 cut(s)	137, 338, 352, 557
AluI	AGCT	4 cut(s)	137, 338, 352, 557
Alw26I	GTCTC	1 cut(s)	597
AlwI	GGATC	2 cut(s)	22, 189
AoxI	GGCC	1 cut(s)	484
ApeKI	GCWGC	1 cut(s)	630
Asp700I	GAANNNNTTC	1 cut(s)	259
AspLEI	GCGC	1 cut(s)	424
AspS9I	GGNCC	3 cut(s)	76, 119, 484
AsuHPI	GGTGA	1 cut(s)	207
AvaII	GGWCC	2 cut(s)	76, 119
BaeGI	GKGCMC	1 cut(s)	444
BanI	GGYRCC	2 cut(s)	315, 454
BccI	CCATC	1 cut(s)	617
BciVI	GTATCC	1 cut(s)	382
BcoDI	GTCTC	1 cut(s)	597
BfaI	CTAG	1 cut(s)	18
BfmI	CTRYAG	1 cut(s)	580
BfoI	RGCGCY	1 cut(s)	425
BfuAI	ACCTGC	1 cut(s)	587
BfuI	GTATCC	1 cut(s)	382
BglII	AGATCT	1 cut(s)	328
BisI	GCNGC	1 cut(s)	631
BlsI	GCNGC	1 cut(s)	632
Bme18I	GGWCC	2 cut(s)	76, 119
BmgT120I	GGNCC	3 cut(s)	76, 119, 484
BmiI	GGNNCC	3 cut(s)	111, 317, 456
BmrI	ACTGGG	1 cut(s)	355
BmsI	GCATC	1 cut(s)	37
BmuI	ACTGGG	1 cut(s)	355
BpmI	CTGGAG	1 cut(s)	399
BpuEI	CTTGAG	2 cut(s)	123, 141
BsaJI	CCNNGG	1 cut(s)	218
Bsc4I	CCNNNNNNNGG	1 cut(s)	451
Bse1I	ACTGG	4 cut(s)	121, 350, 416, 487
Bse3DI	GCAATG	1 cut(s)	122
BseDI	CCNNGG	1 cut(s)	218
BseLI	CCNNNNNNNGG	1 cut(s)	451
BseMI	GCAATG	1 cut(s)	122
BseNI	ACTGG	4 cut(s)	121, 350, 416, 487
BseRI	GAGGAG	1 cut(s)	84
BseSI	GKGCMC	1 cut(s)	444
Bsh1236I	CGCG	1 cut(s)	99
BshFI	GGCC	1 cut(s)	486
BshNI	GGYRCC	2 cut(s)	315, 454
BslI	CCNNNNNNNGG	1 cut(s)	451
BsmAI	GTCTC	1 cut(s)	597
BsnI	GGCC	1 cut(s)	486
Bsp1286I	GDGCHC	1 cut(s)	444
Bsp143I	GATC	3 cut(s)	14, 181, 328
Bsp68I	TCGCGA	1 cut(s)	99
BspACI	CCGC	3 cut(s)	89, 179, 446
BspANI	GGCC	1 cut(s)	486
BspFNI	CGCG	1 cut(s)	99
BspLI	GGNNCC	3 cut(s)	111, 317, 456
BspMAI	CTGCAG	1 cut(s)	584
BspMI	ACCTGC	1 cut(s)	587
BspPI	GGATC	2 cut(s)	22, 189
BspT107I	GGYRCC	2 cut(s)	315, 454
BsrDI	GCAATG	1 cut(s)	122
BsrI	ACTGG	4 cut(s)	121, 350, 416, 487
BssECI	CCNNGG	1 cut(s)	218
BssMI	GATC	3 cut(s)	14, 181, 328
BssT1I	CCWWGG	1 cut(s)	218
Bst4CI	ACNGT	2 cut(s)	175, 275
BstAPI	GCANNNNNTGC	1 cut(s)	537
BstC8I	GCNNGC	1 cut(s)	584
BstDEI	CTNAG	2 cut(s)	41, 593
BstFNI	CGCG	1 cut(s)	99
BstH2I	RGCGCY	1 cut(s)	425
BstHHI	GCGC	1 cut(s)	424
BstKTI	GATC	3 cut(s)	17, 184, 331
BstMAI	GTCTC	1 cut(s)	597
BstMBI	GATC	3 cut(s)	14, 181, 328
BstMWI	GCNNNNNNNGC	2 cut(s)	537, 579
BstSFI	CTRYAG	1 cut(s)	580
BstSLI	GKGCMC	1 cut(s)	444
BstUI	CGCG	1 cut(s)	99
BstX2I	RGATCY	3 cut(s)	14, 181, 328
BstYI	RGATCY	3 cut(s)	14, 181, 328
BsuI	GTATCC	1 cut(s)	382
BsuRI	GGCC	1 cut(s)	486
BtsIMutI	CAGTG	1 cut(s)	414
BtuMI	TCGCGA	1 cut(s)	99
BveI	ACCTGC	1 cut(s)	587
Cac8I	GCNNGC	1 cut(s)	584
CfoI	GCGC	1 cut(s)	424
Cfr13I	GGNCC	3 cut(s)	76, 119, 484
Csp6I	GTAC	1 cut(s)	6
CviAII	CATG	3 cut(s)	64, 106, 481
CviJI	RGCY	7 cut(s)	110, 137, 338, 352, 486, 551, 557
CviKI_1	RGCY	7 cut(s)	110, 137, 338, 352, 486, 551, 557
CviQI	GTAC	1 cut(s)	6
DdeI	CTNAG	2 cut(s)	41, 593
DpnI	GATC	3 cut(s)	16, 183, 330
DpnII	GATC	3 cut(s)	14, 181, 328
EciI	GGCGGA	1 cut(s)	78
Eco130I	CCWWGG	1 cut(s)	218
Eco47I	GGWCC	2 cut(s)	76, 119
Eco57I	CTGAAG	1 cut(s)	318
EcoT14I	CCWWGG	1 cut(s)	218
EcoT22I	ATGCAT	1 cut(s)	482
ErhI	CCWWGG	1 cut(s)	218
FaeI	CATG	3 cut(s)	67, 109, 484
FatI	CATG	3 cut(s)	63, 105, 480
FauI	CCCGC	1 cut(s)	453
FblI	GTMKAC	1 cut(s)	618
Fnu4HI	GCNGC	1 cut(s)	631
Fsp4HI	GCNGC	1 cut(s)	631
FspBI	CTAG	1 cut(s)	18
GlaI	GCGC	1 cut(s)	423
GluI	GCNGC	1 cut(s)	631
GsuI	CTGGAG	1 cut(s)	399
HaeII	RGCGCY	1 cut(s)	425
HaeIII	GGCC	1 cut(s)	486
HhaI	GCGC	1 cut(s)	424
Hin1II	CATG	3 cut(s)	67, 109, 484
Hin6I	GCGC	1 cut(s)	422
HinP1I	GCGC	1 cut(s)	422
HincII	GTYRAC	1 cut(s)	507
HindII	GTYRAC	1 cut(s)	507
HinfI	GANTC	3 cut(s)	37, 279, 412
HphI	GGTGA	1 cut(s)	207
Hpy166II	GTNNAC	4 cut(s)	8, 149, 507, 619
Hpy188I	TCNGA	2 cut(s)	114, 298
Hpy188III	TCNNGA	2 cut(s)	98, 264
Hpy8I	GTNNAC	4 cut(s)	8, 149, 507, 619
HpyAV	CCTTC	3 cut(s)	281, 367, 632
HpyCH4III	ACNGT	2 cut(s)	175, 275
HpyCH4IV	ACGT	1 cut(s)	503
HpyCH4V	TGCA	6 cut(s)	23, 473, 480, 531, 540, 582
HpyF10VI	GCNNNNNNNGC	2 cut(s)	537, 579
HpyF3I	CTNAG	2 cut(s)	41, 593
HpySE526I	ACGT	1 cut(s)	503
Hsp92II	CATG	3 cut(s)	67, 109, 484
HspAI	GCGC	1 cut(s)	422
Kzo9I	GATC	3 cut(s)	14, 181, 328
LmnI	GCTCC	3 cut(s)	115, 335, 349
LweI	GCATC	1 cut(s)	37
MaeI	CTAG	1 cut(s)	18
MaeII	ACGT	1 cut(s)	503
MaeIII	GTNAC	2 cut(s)	409, 518
MalI	GATC	3 cut(s)	16, 183, 330
MboI	GATC	3 cut(s)	14, 181, 328
MboII	GAAGA	6 cut(s)	242, 264, 279, 311, 509, 517
MflI	RGATCY	3 cut(s)	14, 181, 328
MhlI	GDGCHC	1 cut(s)	444
MluCI	AATT	4 cut(s)	82, 302, 310, 361
MlyI	GAGTC	2 cut(s)	288, 406
MmeI	TCCRAC	1 cut(s)	137
MnlI	CCTC	7 cut(s)	53, 89, 102, 105, 231, 379, 445
Mph1103I	ATGCAT	1 cut(s)	482
MroXI	GAANNNNTTC	1 cut(s)	259
MseI	TTAA	1 cut(s)	637
MvnI	CGCG	1 cut(s)	99
MwoI	GCNNNNNNNGC	2 cut(s)	537, 579
NdeII	GATC	3 cut(s)	14, 181, 328
NlaIII	CATG	3 cut(s)	67, 109, 484
NlaIV	GGNNCC	3 cut(s)	111, 317, 456
NmuCI	GTSAC	2 cut(s)	409, 518
NruI	TCGCGA	1 cut(s)	99
NsiI	ATGCAT	1 cut(s)	482
PdmI	GAANNNNTTC	1 cut(s)	259
PfeI	GAWTC	1 cut(s)	37
PkrI	GCNGC	1 cut(s)	632
PleI	GAGTC	2 cut(s)	287, 406
PpsI	GAGTC	2 cut(s)	287, 406
PspN4I	GGNNCC	3 cut(s)	111, 317, 456
PspPI	GGNCC	3 cut(s)	76, 119, 484
PstI	CTGCAG	1 cut(s)	584
PsuI	RGATCY	3 cut(s)	14, 181, 328
RruI	TCGCGA	1 cut(s)	99
RsaI	GTAC	1 cut(s)	7
RsaNI	GTAC	1 cut(s)	6
SaqAI	TTAA	1 cut(s)	637
SatI	GCNGC	1 cut(s)	631
Sau3AI	GATC	3 cut(s)	14, 181, 328
Sau96I	GGNCC	3 cut(s)	76, 119, 484
SbfI	CCTGCAGG	1 cut(s)	584
SchI	GAGTC	2 cut(s)	288, 406
SdaI	CCTGCAGG	1 cut(s)	584
SduI	GDGCHC	1 cut(s)	444
SfaNI	GCATC	1 cut(s)	37
SfcI	CTRYAG	1 cut(s)	580
SinI	GGWCC	2 cut(s)	76, 119
SmlI	CTYRAG	2 cut(s)	138, 156
SmoI	CTYRAG	2 cut(s)	138, 156
Sse8387I	CCTGCAGG	1 cut(s)	584
Sse9I	AATT	4 cut(s)	82, 302, 310, 361
SsiI	CCGC	3 cut(s)	89, 179, 446
SspMI	CTAG	1 cut(s)	18
StyI	CCWWGG	1 cut(s)	218
TaaI	ACNGT	2 cut(s)	175, 275
TaiI	ACGT	1 cut(s)	506
TaqI	TCGA	4 cut(s)	28, 250, 265, 366
TasI	AATT	4 cut(s)	82, 302, 310, 361
TatI	WGTACW	1 cut(s)	5
TfiI	GAWTC	1 cut(s)	37
Tru1I	TTAA	1 cut(s)	637
Tru9I	TTAA	1 cut(s)	637
TscAI	CASTG	1 cut(s)	414
TseFI	GTSAC	2 cut(s)	409, 518
TseI	GCWGC	1 cut(s)	630
Tsp45I	GTSAC	2 cut(s)	409, 518
TspDTI	ATGAA	1 cut(s)	361
TspRI	CASTG	1 cut(s)	414
VpaK11BI	GGWCC	2 cut(s)	76, 119
XmiI	GTMKAC	1 cut(s)	618
XmnI	GAANNNNTTC	1 cut(s)	259
XspI	CTAG	1 cut(s)	18
Zsp2I	ATGCAT	1 cut(s)	482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.