Rh4BG183600

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001134
Physical Location & Seq
Reverse (-)
32186312 .. 32188976
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG183600.1

Sequence Viewer

Length: 666 bp
ATGGCCTCACACTCTTCAGAGGAATGTTCTCAAAGGGGGAAGAGGCCACTAACTATAGACCTCAACTATCCAGCTCCGTGTGAGGAGGTGGTTCTAAATACCAGATTTGCCTGGGCTAATAGCCAACAAGGTCAAGATGAAGGACATGGTCGGTTAGCACCACAAACTTCAAATGATTGTGAGGTCATTGATGAGGACATTGCTTTCATATCCCCGGTGATATATGCCCAATCCAAGAATAGTTCCAGAAGAAACCTTGAGGTGAGGGACGTCGTAGCTACCAATACAGAAATGCATAGTGGCCATTCAGACTGGGGTGCTCAGATTCCTCAAATTCCCCTTCATTGCCACAAGCGCAAAAGAACTTTACGAAATCAAGCAGTTTTGAATTGGGAGCTTCACTTTAGCTCAGAAGATGGTGACAAGAACAAGATAGAGGAAGTACCTTCTCCTGAGCTTCCTGAGAGCACACCACCTCAAGAGATACCTTCCTTCAGCTGCCCAATATGTTTGGGCTCTTTAAGTGAAGAAACATCGACGAAATGTGGTCACATCTTCTGCAAGATGTGTATTGAAGCATCAATAAAAGTTCAGCACAAATGCCCAACTTGTCGTAAAAAGCTTAGAATGAAAGACACCATCAGGGTGTACCTTCCTACCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
zf-C3HC4_2 zf-RING_2

Protein Analysis

221

Amino Acids

24.91

Weight (kDa)

6.5

Isoelectric Point (pI)

74.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 166 - 205 5.7e-09 Ring finger domain
zf-C3HC4_3 PF13920 166 - 208 6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 167 - 204 2.8e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 167 - 204 4e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 167 - 204 7.2e-06 zinc finger of C3HC4-type, RING
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 1 cut(s) 333
AcuI CTGAAG 1 cut(s) 478
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 2 cut(s) 444, 650
AgsI TTSAA 3 cut(s) 171, 388, 575
AjnI CCWGG 1 cut(s) 110
AluBI AGCT 7 cut(s) 74, 278, 397, 408, 457, 498, 622
AluI AGCT 7 cut(s) 74, 278, 397, 408, 457, 498, 622
Alw21I GWGCWC 2 cut(s) 322, 470
AoxI GGCC 3 cut(s) 3, 44, 301
ApeKI GCWGC 1 cut(s) 498
ApoI RAATTY 1 cut(s) 333
AspLEI GCGC 1 cut(s) 357
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 229, 274, 431
BalI TGGCCA 1 cut(s) 303
BanII GRGCYC 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 322, 470
BbvI GCAGC 1 cut(s) 485
BccI CCATC 2 cut(s) 410, 647
BciT130I CCWGG 1 cut(s) 112
BcnI CCSGG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 54
BisI GCNGC 1 cut(s) 499
BlsI GCNGC 1 cut(s) 500
Bme1390I CCNGG 2 cut(s) 112, 215
BmrFI CCNGG 2 cut(s) 112, 215
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 1 cut(s) 587
BmuI ACTGGG 1 cut(s) 322
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 2 cut(s) 278, 462
BpuMI CCSGG 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 2 cut(s) 111, 213
Bse1I ACTGG 1 cut(s) 317
Bse3DI GCAATG 2 cut(s) 198, 343
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 111, 213
BseMI GCAATG 2 cut(s) 198, 343
BseMII CTCAG 4 cut(s) 335, 423, 444, 453
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 1 cut(s) 485
BshFI GGCC 3 cut(s) 5, 46, 303
BsiHKAI GWGCWC 2 cut(s) 322, 470
BsiSI CCGG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 3 cut(s) 5, 46, 303
Bsp1286I GDGCHC 3 cut(s) 322, 470, 518
BspANI GGCC 3 cut(s) 5, 46, 303
BspCNI CTCAG 4 cut(s) 334, 422, 445, 454
BsrDI GCAATG 2 cut(s) 198, 343
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 111, 213
BssNI GRCGYC 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 112
Bst6I CTCTTC 2 cut(s) 19, 35
BstACI GRCGYC 1 cut(s) 270
BstDEI CTNAG 5 cut(s) 321, 409, 453, 462, 623
BstHHI GCGC 1 cut(s) 357
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstNI CCWGG 1 cut(s) 112
BstSCI CCNGG 2 cut(s) 110, 213
BstSFI CTRYAG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 485
BsuRI GGCC 3 cut(s) 5, 46, 303
CfoI GCGC 1 cut(s) 357
Csp6I GTAC 2 cut(s) 443, 649
CviAII CATG 1 cut(s) 146
CviQI GTAC 2 cut(s) 443, 649
DdeI CTNAG 5 cut(s) 321, 409, 453, 462, 623
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 19, 35
EarI CTCTTC 2 cut(s) 19, 35
Eco24I GRGCYC 1 cut(s) 518
Eco57I CTGAAG 1 cut(s) 478
EcoRII CCWGG 1 cut(s) 110
EcoT22I ATGCAT 1 cut(s) 297
EcoT38I GRGCYC 1 cut(s) 518
FaeI CATG 1 cut(s) 149
FaiI YATR 7 cut(s) 56, 147, 209, 223, 225, 297, 508
FaqI GGGAC 1 cut(s) 281
FatI CATG 1 cut(s) 145
Fnu4HI GCNGC 1 cut(s) 499
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 1 cut(s) 499
GlaI GCGC 1 cut(s) 356
GluI GCNGC 1 cut(s) 499
HaeIII GGCC 3 cut(s) 5, 46, 303
HapII CCGG 1 cut(s) 215
HhaI GCGC 1 cut(s) 357
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 1 cut(s) 149
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 1 cut(s) 325
HpaII CCGG 1 cut(s) 215
HphI GGTGA 3 cut(s) 229, 274, 431
Hpy166II GTNNAC 1 cut(s) 649
Hpy188I TCNGA 4 cut(s) 19, 310, 324, 412
Hpy188III TCNNGA 5 cut(s) 134, 246, 452, 461, 479
Hpy8I GTNNAC 1 cut(s) 649
Hpy99I CGWCG 2 cut(s) 275, 541
HpyAV CCTTC 6 cut(s) 134, 350, 456, 498, 502, 662
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 2 cut(s) 295, 561
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 5 cut(s) 321, 409, 453, 462, 623
HpySE526I ACGT 1 cut(s) 270
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 1 cut(s) 149
HspAI GCGC 1 cut(s) 355
LmnI GCTCC 2 cut(s) 79, 394
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 1 cut(s) 587
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 2 cut(s) 419, 548
MboII GAAGA 6 cut(s) 6, 52, 261, 425, 539, 547
MhlI GDGCHC 3 cut(s) 322, 470, 518
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 2 cut(s) 333, 388
MluNI TGGCCA 1 cut(s) 303
Mox20I TGGCCA 1 cut(s) 303
Mph1103I ATGCAT 1 cut(s) 297
MscI TGGCCA 1 cut(s) 303
MseI TTAA 1 cut(s) 521
MslI CAYNNNNRTG 1 cut(s) 644
Msp20I TGGCCA 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 498
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 2 cut(s) 112, 215
MvaI CCWGG 1 cut(s) 112
MwoI GCNNNNNNNGC 1 cut(s) 354
NciI CCSGG 1 cut(s) 215
NlaIII CATG 1 cut(s) 149
NmuCI GTSAC 2 cut(s) 419, 548
NsiI ATGCAT 1 cut(s) 297
PfeI GAWTC 1 cut(s) 325
PflFI GACNNNGTC 1 cut(s) 147
PkrI GCNGC 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 110
PspGI CCWGG 1 cut(s) 110
PsyI GACNNNGTC 1 cut(s) 147
PvuII CAGCTG 1 cut(s) 498
RsaI GTAC 2 cut(s) 444, 650
RsaNI GTAC 2 cut(s) 443, 649
RseI CAYNNNNRTG 1 cut(s) 644
SaqAI TTAA 1 cut(s) 521
SatI GCNGC 1 cut(s) 499
ScrFI CCNGG 2 cut(s) 112, 215
SduI GDGCHC 3 cut(s) 322, 470, 518
SfaNI GCATC 1 cut(s) 587
SfcI CTRYAG 1 cut(s) 54
SmiMI CAYNNNNRTG 1 cut(s) 644
SmlI CTYRAG 2 cut(s) 257, 477
SmoI CTYRAG 2 cut(s) 257, 477
Sse9I AATT 2 cut(s) 333, 388
StyD4I CCNGG 2 cut(s) 110, 213
TaiI ACGT 1 cut(s) 273
TaqI TCGA 1 cut(s) 536
TasI AATT 2 cut(s) 333, 388
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TseFI GTSAC 2 cut(s) 419, 548
TseI GCWGC 1 cut(s) 498
Tsp45I GTSAC 2 cut(s) 419, 548
TspDTI ATGAA 4 cut(s) 153, 196, 332, 644
TspGWI ACGGA 1 cut(s) 66
Tth111I GACNNNGTC 1 cut(s) 147
XapI RAATTY 1 cut(s) 333
ZraI GACGTC 1 cut(s) 271
Zsp2I ATGCAT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.