RLG00000008179

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001134
Physical Location & Seq
Forward (+)
26671990 .. 26673912
1923 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008179

Sequence Viewer

Length: 666 bp
ATGAATCAGCTTCTTGGCATGAGCATAGGAGGATCGAGTTACTTTCAACAAGTGAATGAGAGTGAGGCTGAAAGAGCGAGAAGATTGGCAAGGGTAGTAGATTTGGACCTTAACCACCCACCTCCTAGTGAGAACCTGGCTCTAGCTGCTCCTCCGAGCGTTCAACCTCAACAACAATCTTCGGACGCTCCTGATGATGAATGTCTAATCATATCACCACGGAAGTTTGAAGAGGCCATGAATAATTCTCAGAGAAATCCGTCTCGCAGAGTAAGAAGCAATATAGAAGATTTTACTGAAGTTGGTGTTGCACAATCATTTGATTTCTTATCAACAGACTTGATAACATGGGGGATCTCCTTTGGCAGACTTGGAACTGTCAACGGAGTGATTTCCTTGAACGAAGAAGTTCTTAGCAAAACTAAAGATACTGAAACCAGTATCCCCATGGCTCCAATAGAGCCCCAAAGTGAGCCTGAGGCACCTGCGTTTTCTTGTGTAATCTGCATGGGCCAATTGGTTGAAGAAACATCAACAAAATGCGGCCACATCTTCTGCAAGATGTGTATTGAAAAAGCAATAGCTACTCAACACAAATGCCCTAACTGCAGGCGTAAACTTAGAAAGCGAGACATCTTCAGGGTCCACCTTCCATACACCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_2

Protein Analysis

222

Amino Acids

24.61

Weight (kDa)

5.65

Isoelectric Point (pI)

76.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 165 - 204 3.5e-09 Ring finger domain
zf-C3HC4_3 PF13920 165 - 209 2.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 166 - 203 3.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 166 - 203 4.5e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 493
Acc36I ACCTGC 1 cut(s) 493
AccB1I GGYRCC 1 cut(s) 481
AciI CCGC 1 cut(s) 543
AclWI GGATC 2 cut(s) 40, 362
AcoI YGGCCR 1 cut(s) 544
AcuI CTGAAG 2 cut(s) 318, 622
AgsI TTSAA 6 cut(s) 47, 164, 230, 400, 524, 572
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 3 cut(s) 10, 146, 584
AluI AGCT 3 cut(s) 10, 146, 584
Alw26I GTCTC 2 cut(s) 267, 624
AlwI GGATC 2 cut(s) 40, 362
AoxI GGCC 3 cut(s) 234, 511, 544
ApeKI GCWGC 1 cut(s) 146
Asp700I GAANNNNTTC 1 cut(s) 408
AspS9I GGNCC 3 cut(s) 106, 511, 643
AsuHPI GGTGA 1 cut(s) 207
AvaII GGWCC 2 cut(s) 106, 643
AxyI CCTNAGG 1 cut(s) 477
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 137
BciVI GTATCC 1 cut(s) 452
BcoDI GTCTC 2 cut(s) 267, 624
BfaI CTAG 2 cut(s) 126, 143
BfmI CTRYAG 1 cut(s) 607
BfuAI ACCTGC 1 cut(s) 493
BfuI GTATCC 1 cut(s) 452
BisI GCNGC 2 cut(s) 147, 544
BlsI GCNGC 2 cut(s) 148, 545
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 2 cut(s) 106, 643
BmgT120I GGNCC 3 cut(s) 106, 511, 643
BmiI GGNNCC 3 cut(s) 453, 483, 644
BmrFI CCNGG 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 218, 447
Bse1I ACTGG 2 cut(s) 438, 660
Bse21I CCTNAGG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 218, 447
BseMII CTCAG 2 cut(s) 263, 468
BseNI ACTGG 2 cut(s) 438, 660
BseRI GAGGAG 1 cut(s) 141
BseXI GCAGC 1 cut(s) 133
BshFI GGCC 3 cut(s) 236, 513, 546
BshNI GGYRCC 1 cut(s) 481
BsmAI GTCTC 2 cut(s) 267, 624
BsmBI CGTCTC 1 cut(s) 267
BsnI GGCC 3 cut(s) 236, 513, 546
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 2 cut(s) 32, 354
Bsp19I CCATGG 1 cut(s) 447
BspACI CCGC 1 cut(s) 543
BspANI GGCC 3 cut(s) 236, 513, 546
BspCNI CTCAG 2 cut(s) 262, 469
BspLI GGNNCC 3 cut(s) 453, 483, 644
BspMAI CTGCAG 1 cut(s) 611
BspMI ACCTGC 1 cut(s) 493
BspPI GGATC 2 cut(s) 40, 362
BspT107I GGYRCC 1 cut(s) 481
BsrI ACTGG 2 cut(s) 438, 660
BssECI CCNNGG 2 cut(s) 218, 447
BssMI GATC 2 cut(s) 32, 354
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 611
BstDEI CTNAG 4 cut(s) 249, 413, 477, 620
BstDSI CCRYGG 2 cut(s) 218, 447
BstKTI GATC 2 cut(s) 35, 357
BstMAI GTCTC 2 cut(s) 267, 624
BstMBI GATC 2 cut(s) 32, 354
BstMWI GCNNNNNNNGC 3 cut(s) 74, 146, 606
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 607
BstV1I GCAGC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
Bsu36I CCTNAGG 1 cut(s) 477
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 3 cut(s) 236, 513, 546
BtgI CCRYGG 2 cut(s) 218, 447
BveI ACCTGC 1 cut(s) 493
Cac8I GCNNGC 1 cut(s) 611
Cfr13I GGNCC 3 cut(s) 106, 511, 643
CseI GACGC 1 cut(s) 194
CviAII CATG 5 cut(s) 19, 238, 348, 448, 508
DdeI CTNAG 4 cut(s) 249, 413, 477, 620
DpnI GATC 2 cut(s) 34, 356
DpnII GATC 2 cut(s) 32, 354
EaeI YGGCCR 1 cut(s) 544
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 465
Eco47I GGWCC 2 cut(s) 106, 643
Eco57I CTGAAG 2 cut(s) 318, 622
Eco81I CCTNAGG 1 cut(s) 477
EcoRII CCWGG 1 cut(s) 135
EcoT14I CCWWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 267
FaeI CATG 5 cut(s) 22, 241, 351, 451, 511
FaiI YATR 9 cut(s) 20, 26, 212, 239, 284, 349, 449, 509, 655
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FatI CATG 5 cut(s) 18, 237, 347, 447, 507
Fnu4HI GCNGC 2 cut(s) 147, 544
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 2 cut(s) 147, 544
FspBI CTAG 2 cut(s) 126, 143
GluI GCNGC 2 cut(s) 147, 544
HaeIII GGCC 3 cut(s) 236, 513, 546
HgaI GACGC 1 cut(s) 194
Hin1II CATG 5 cut(s) 22, 241, 351, 451, 511
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 1 cut(s) 4
HphI GGTGA 1 cut(s) 207
Hpy166II GTNNAC 3 cut(s) 382, 617, 646
Hpy188I TCNGA 3 cut(s) 156, 184, 252
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 3 cut(s) 382, 617, 646
HpyAV CCTTC 1 cut(s) 659
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4V TGCA 4 cut(s) 311, 507, 558, 609
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 146, 606
HpyF3I CTNAG 4 cut(s) 249, 413, 477, 620
Hsp92II CATG 5 cut(s) 22, 241, 351, 451, 511
Kzo9I GATC 2 cut(s) 32, 354
LmnI GCTCC 3 cut(s) 154, 193, 457
LpnPI CCDG 8 cut(s) 122, 149, 204, 451, 489, 498, 595, 625
Lsp1109I GCAGC 1 cut(s) 133
MaeI CTAG 2 cut(s) 126, 143
MaeIII GTNAC 1 cut(s) 38
MalI GATC 2 cut(s) 34, 356
MboI GATC 2 cut(s) 32, 354
MboII GAAGA 8 cut(s) 93, 171, 242, 299, 416, 536, 544, 628
MfeI CAATTG 1 cut(s) 515
MflI RGATCY 1 cut(s) 354
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 2 cut(s) 244, 515
MnlI CCTC 7 cut(s) 23, 58, 132, 162, 177, 226, 472
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 2 cut(s) 111, 664
MspR9I CCNGG 1 cut(s) 137
MunI CAATTG 1 cut(s) 515
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 74, 146, 606
NcoI CCATGG 1 cut(s) 447
NdeII GATC 2 cut(s) 32, 354
NlaIII CATG 5 cut(s) 22, 241, 351, 451, 511
NlaIV GGNNCC 3 cut(s) 453, 483, 644
PaqCI CACCTGC 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 2 cut(s) 148, 545
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 3 cut(s) 453, 483, 644
PspPI GGNCC 3 cut(s) 106, 511, 643
PstI CTGCAG 1 cut(s) 611
PsuI RGATCY 1 cut(s) 354
SaqAI TTAA 2 cut(s) 111, 664
SatI GCNGC 2 cut(s) 147, 544
Sau3AI GATC 2 cut(s) 32, 354
Sau96I GGNCC 3 cut(s) 106, 511, 643
ScrFI CCNGG 1 cut(s) 137
SduI GDGCHC 1 cut(s) 465
SetI ASST 9 cut(s) 12, 111, 124, 138, 148, 169, 487, 586, 651
SfcI CTRYAG 1 cut(s) 607
SinI GGWCC 2 cut(s) 106, 643
Sse9I AATT 2 cut(s) 244, 515
SsiI CCGC 1 cut(s) 543
SspMI CTAG 2 cut(s) 126, 143
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 1 cut(s) 379
TaqI TCGA 1 cut(s) 35
TasI AATT 2 cut(s) 244, 515
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 111, 664
Tru9I TTAA 2 cut(s) 111, 664
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 3 cut(s) 17, 213, 254
TspGWI ACGGA 3 cut(s) 235, 249, 399
VpaK11BI GGWCC 2 cut(s) 106, 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 2 cut(s) 126, 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.