MD04G1013800.v1.1

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001134
Physical Location & Seq
Forward (+)
1572466 .. 1576758
4293 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1013800.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGCCTCACACTCATTGGGGCAATCTACAGAAGGGAAAATGAGGAATTCTCAAAGGGGGAAGATGCCACTAATTGTGGACCTCAACTATCCAGCTCCTTGTGAGGACATGGTTCTAAATGCCAGATGTTCTTGGGACAATAGTCAACAAGTTCAGGTTGAAATATATGGTCGGTCAGAAGCACAAGCTTCAAATGATTGTGAGGTTGTCGATGATGAAGTTGTCATAATTTCCCCAAGGGTATATGCTCAAGCCAAGAATAACTCCAGGAGAAACCTTGAGGTGAGAGATGGGTCTGCTACAAACTCTGAAAGGCAAAATGGCCATACAGATGTGCCTCCTCAATTACCTGTACCTCTACTTCCTCTTCATTGCCACAAGCGTAGAAGAACTTTACGAAATCAAGCAGTTTTGAATTGGGAGCATCATTTTAGCTTGGAAGACAATGACGAAAACAAGACAAAGGAAGTGTCTATTCCAGAACTTGCTGAGAACACGCCACCCCCAGAGACACCCTCCTTCAGCTGCCCAATATGCTTGGGCCCGTTGAATGAAGAAACCTCAACAAAGTGCGGTCACATCTTCTGCAAGATGTGTATTGAAGCCTCAATAAAAGTTCAGCACAAATGCCCAACTTGTCGTAAAAGGCTTAGAAAGAAAGACACCATCAGGATATACCTGCCTACCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
zf-C3HC4_2 zf-RING_2

Protein Analysis

231

Amino Acids

26.07

Weight (kDa)

8.0

Isoelectric Point (pI)

62.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 175 - 214 5.8e-09 Ring finger domain
zf-C3HC4_2 PF13923 176 - 213 2.3e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 176 - 213 7.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 176 - 211 8.7e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 687
AciI CCGC 1 cut(s) 573
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 505
AfaI GTAC 1 cut(s) 354
AgsI TTSAA 5 cut(s) 161, 192, 415, 550, 602
AjnI CCWGG 1 cut(s) 266
AluBI AGCT 4 cut(s) 95, 188, 435, 525
AluI AGCT 4 cut(s) 95, 188, 435, 525
Alw26I GTCTC 1 cut(s) 503
AoxI GGCC 3 cut(s) 3, 322, 541
ApaI GGGCCC 1 cut(s) 545
ApeKI GCWGC 1 cut(s) 525
ApoI RAATTY 1 cut(s) 46
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
AspS9I GGNCC 3 cut(s) 79, 541, 542
AsuHPI GGTGA 1 cut(s) 295
AvaII GGWCC 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 545
BalI TGGCCA 1 cut(s) 324
BanII GRGCYC 1 cut(s) 545
BarI GAAGNNNNNNTAC 2 cut(s) 345, 377
BbsI GAAGAC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 512
BccI CCATC 2 cut(s) 284, 674
BciT130I CCWGG 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 503
BfmI CTRYAG 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 687
BisI GCNGC 1 cut(s) 526
BlsI GCNGC 1 cut(s) 527
Bme1390I CCNGG 1 cut(s) 268
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 3 cut(s) 79, 541, 542
BmiI GGNNCC 1 cut(s) 543
BmrFI CCNGG 1 cut(s) 268
BmsI GCATC 2 cut(s) 54, 433
BoxI GACNNNNGTC 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 447
BplI GAGNNNNNCTC 4 cut(s) 34, 66, 500, 532
BpmI CTGGAG 1 cut(s) 250
BpuEI CTTGAG 2 cut(s) 234, 299
BsaJI CCNNGG 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 370
BseBI CCWGG 1 cut(s) 268
BseDI CCNNGG 1 cut(s) 236
BseMI GCAATG 1 cut(s) 370
BseMII CTCAG 1 cut(s) 480
BseRI GAGGAG 1 cut(s) 330
BseSI GKGCMC 1 cut(s) 545
BseXI GCAGC 1 cut(s) 512
BshFI GGCC 3 cut(s) 5, 324, 543
BslFI GGGAC 1 cut(s) 149
BsmAI GTCTC 1 cut(s) 503
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 3 cut(s) 5, 324, 543
Bsp120I GGGCCC 1 cut(s) 541
Bsp1286I GDGCHC 1 cut(s) 545
BspACI CCGC 1 cut(s) 573
BspANI GGCC 3 cut(s) 5, 324, 543
BspCNI CTCAG 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 543
BspMI ACCTGC 1 cut(s) 687
BsrDI GCAATG 1 cut(s) 370
BssECI CCNNGG 1 cut(s) 236
BssT1I CCWWGG 1 cut(s) 236
Bst2UI CCWGG 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 372
BstDEI CTNAG 2 cut(s) 489, 650
BstMAI GTCTC 1 cut(s) 503
BstMWI GCNNNNNNNGC 1 cut(s) 534
BstNI CCWGG 1 cut(s) 268
BstPAI GACNNNNGTC 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 266
BstSFI CTRYAG 1 cut(s) 27
BstSLI GKGCMC 1 cut(s) 545
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 1 cut(s) 447
BsuRI GGCC 3 cut(s) 5, 324, 543
BveI ACCTGC 1 cut(s) 687
Cfr13I GGNCC 3 cut(s) 79, 541, 542
Csp6I GTAC 1 cut(s) 353
CviAII CATG 1 cut(s) 109
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 2 cut(s) 489, 650
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 236
EcoT38I GRGCYC 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 1 cut(s) 112
FaiI YATR 9 cut(s) 110, 166, 168, 227, 244, 246, 327, 535, 676
FaqI GGGAC 1 cut(s) 149
FatI CATG 1 cut(s) 108
Fnu4HI GCNGC 1 cut(s) 526
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 1 cut(s) 526
GluI GCNGC 1 cut(s) 526
GsuI CTGGAG 1 cut(s) 250
HaeIII GGCC 3 cut(s) 5, 324, 543
Hin1II CATG 1 cut(s) 112
HincII GTYRAC 1 cut(s) 146
HindII GTYRAC 1 cut(s) 146
HindIII AAGCTT 1 cut(s) 186
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 79, 146
Hpy188I TCNGA 2 cut(s) 178, 310
Hpy188III TCNNGA 2 cut(s) 479, 670
Hpy8I GTNNAC 2 cut(s) 79, 146
HpyAV CCTTC 2 cut(s) 26, 529
HpyCH4V TGCA 1 cut(s) 588
HpyF10VI GCNNNNNNNGC 1 cut(s) 534
HpyF3I CTNAG 2 cut(s) 489, 650
Hsp92II CATG 1 cut(s) 112
LmnI GCTCC 2 cut(s) 100, 421
LpnPI CCDG 9 cut(s) 105, 136, 140, 253, 280, 363, 492, 519, 655
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 2 cut(s) 54, 433
MaeIII GTNAC 1 cut(s) 575
MboII GAAGA 6 cut(s) 73, 359, 399, 452, 566, 574
MhlI GDGCHC 1 cut(s) 545
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 5 cut(s) 46, 72, 228, 344, 415
MluNI TGGCCA 1 cut(s) 324
Mox20I TGGCCA 1 cut(s) 324
MscI TGGCCA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 330
Msp20I TGGCCA 1 cut(s) 324
MspA1I CMGCKG 1 cut(s) 525
MspR9I CCNGG 1 cut(s) 268
MvaI CCWGG 1 cut(s) 268
MwoI GCNNNNNNNGC 1 cut(s) 534
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 1 cut(s) 543
NmuCI GTSAC 1 cut(s) 575
PfoI TCCNGGA 1 cut(s) 266
PkrI GCNGC 1 cut(s) 527
PshAI GACNNNNGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 543
PspOMI GGGCCC 1 cut(s) 541
PspPI GGNCC 3 cut(s) 79, 541, 542
PvuII CAGCTG 1 cut(s) 525
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
RseI CAYNNNNRTG 1 cut(s) 330
SatI GCNGC 1 cut(s) 526
Sau96I GGNCC 3 cut(s) 79, 541, 542
ScrFI CCNGG 1 cut(s) 268
SduI GDGCHC 1 cut(s) 545
SfaNI GCATC 2 cut(s) 54, 433
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 2 cut(s) 249, 278
SmoI CTYRAG 2 cut(s) 249, 278
Sse9I AATT 5 cut(s) 46, 72, 228, 344, 415
SsiI CCGC 1 cut(s) 573
StyD4I CCNGG 1 cut(s) 266
StyI CCWWGG 1 cut(s) 236
TaqI TCGA 1 cut(s) 210
TaqII GACCGA 1 cut(s) 162
TasI AATT 5 cut(s) 46, 72, 228, 344, 415
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 1 cut(s) 525
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 3 cut(s) 231, 359, 567
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.