Rorug04G0125400

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001134
Physical Location & Seq
Forward (+)
19875210 .. 19877240
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0125400.1

Sequence Viewer

Length: 1080 bp
ATGGGACTTCAAGCCGGTTGGACCCTGCCGGGGCTCACCCCCAAGCCCTGCGACACCTGCAAGTCGTCGCCGTCGGCCGTCTTCTGCCGCGCCGACTCGGCCTACCTCTGCCTGACCTGCGACTCCAAGATCCACTGCGCCAACAAGCTCGCGTCGCGCCACCAGCGCGTCTGGATGTGCGAGGTCTGCGAGCAAGCTCCGGCCGCCGTCACGTGCAAGGCCGACGCCGCCGCCCTCTGCGTCACGTGCGACGCCGACATCCACTCCGCCAATCCACTCGCGCGTCGCCACGAGCGCGTCCCCGTGGAGCCGTTCCTAGACTCGGCCGAGTCCATCGTCAAATCCACCTCGGCTACTGCTGGCCTCAACGACGCCGTTTCTCCTCTTAAAAACGAAGACGCCGATTCTCTGCTCATTCCCAACCTGAATTTCAGCTCCAAGTTCATTGATGCTCTCGACATCAAGCCCGGGGATATGTTCTTCCCGGAAATGGAATCGTTATTCGAACTCGATTACCCGAACCCGATCCAAAACTCCAGTTCGGGTATGGATAGCGTGGTTCCGGTTCAACCCGACCCGATTCCACTTCCTCCTGTCGTCAACCAACCCCCTCATGAAAATTGCTTCGACATTGACTTCTGCAGAAACAAGCTCTCTTCCTCCTTCAGTTATCCAGCTCAATCAATCAGCCAAAGCGTCTCGTCGTCCTCCTTAGAGGTGGGAGTTGTTCCCGACGGAAATTCATTGTCCGACATTTCATATCCGTTCATCCAAAACGTGAACCACAACTGCGTGGAAGCCGGCGTAGCGGCTGCTCCGGCGACGGCGACAGCGACTCAGCTGAGCGGAGTGGATCGCGAGGCGAGAGTGATGAGGTACAGAGAGAAGAGGAAGAACCGCAAGTTCCAGAAGACAATCCGATACGCCTCCCGGAAAGCCTATGCCGAGACCCGGCCGAGAATCAAAGGCCGGTTCGCGAAACGCACCGAGACCGAAACGGAGGTGATGGATCGGATCTTTAACTCGGCGCCGGAGAGTTATATTGCGGACGTACAATACGGCGTCGTCCCGACGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
zf-C3HC4_2 zf-RING_2

Protein Analysis

359

Amino Acids

39.27

Weight (kDa)

5.76

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 56 - 102 1.4e-06 B-box zinc finger
CCT PF06203 286 - 328 4.8e-18 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 65
Acc36I ACCTGC 2 cut(s) 65, 125
AccB1I GGYRCC 1 cut(s) 1027
AccBSI CCGCTC 1 cut(s) 846
AccII CGCG 9 cut(s) 90, 152, 157, 168, 281, 283, 297, 858, 977
AciI CCGC 9 cut(s) 88, 204, 228, 231, 267, 809, 846, 898, 1046
AclWI GGATC 5 cut(s) 124, 520, 861, 1017, 1022
AcoI YGGCCR 4 cut(s) 75, 201, 324, 953
AcsI RAATTY 2 cut(s) 427, 739
AcuI CTGAAG 1 cut(s) 649
AcvI CACGTG 2 cut(s) 213, 246
AcyI GRCGYC 6 cut(s) 225, 252, 372, 399, 1028, 1062
AfaI GTAC 2 cut(s) 878, 1053
AfiI CCNNNNNNNGG 4 cut(s) 30, 322, 490, 951
AgsI TTSAA 2 cut(s) 11, 569
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AluBI AGCT 6 cut(s) 148, 197, 435, 652, 677, 841
AluI AGCT 6 cut(s) 148, 197, 435, 652, 677, 841
Alw26I GTCTC 3 cut(s) 703, 941, 983
AlwI GGATC 5 cut(s) 124, 520, 861, 1017, 1022
Ama87I CYCGRG 1 cut(s) 467
AoxI GGCC 8 cut(s) 75, 99, 201, 219, 324, 361, 953, 967
ApeKI GCWGC 1 cut(s) 812
ApoI RAATTY 2 cut(s) 427, 739
AspLEI GCGC 7 cut(s) 92, 140, 159, 168, 283, 297, 1030
AspS9I GGNCC 1 cut(s) 21
AsuC2I CCSGG 6 cut(s) 30, 468, 469, 485, 931, 952
AsuHPI GGTGA 2 cut(s) 28, 1015
AsuII TTCGAA 1 cut(s) 504
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 21
BanI GGYRCC 1 cut(s) 1027
BanII GRGCYC 1 cut(s) 36
BauI CACGAG 1 cut(s) 290
BbrPI CACGTG 2 cut(s) 213, 246
BbsI GAAGAC 3 cut(s) 73, 402, 917
BbvI GCAGC 1 cut(s) 799
BccI CCATC 2 cut(s) 341, 1000
BceAI ACGGC 7 cut(s) 55, 62, 191, 295, 359, 840, 1075
BcnI CCSGG 6 cut(s) 30, 468, 469, 485, 931, 952
BcoDI GTCTC 3 cut(s) 703, 941, 983
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 640
BfoI RGCGCY 1 cut(s) 1031
BfuAI ACCTGC 2 cut(s) 65, 125
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 6 cut(s) 88, 204, 228, 231, 810, 813
BlpI GCTNAGC 1 cut(s) 842
BlsI GCNGC 6 cut(s) 89, 205, 229, 232, 811, 814
Bme1390I CCNGG 6 cut(s) 30, 468, 469, 485, 931, 952
Bme18I GGWCC 1 cut(s) 21
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 4 cut(s) 23, 309, 561, 1029
BmrFI CCNGG 6 cut(s) 30, 468, 469, 485, 931, 952
BmsI GCATC 1 cut(s) 439
BpiI GAAGAC 3 cut(s) 73, 402, 917
BpmI CTGGAG 1 cut(s) 520
Bpu1102I GCTNAGC 1 cut(s) 842
Bpu14I TTCGAA 1 cut(s) 504
BpuMI CCSGG 6 cut(s) 30, 468, 469, 485, 931, 952
BsaAI YACGTR 2 cut(s) 213, 246
BsaHI GRCGYC 6 cut(s) 225, 252, 372, 399, 1028, 1062
BsaI GGTCTC 2 cut(s) 941, 983
BsaJI CCNNGG 5 cut(s) 29, 303, 348, 467, 468
BsaWI WCCGGW 1 cut(s) 562
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 322, 490, 951
Bse118I RCCGGY 3 cut(s) 14, 800, 969
Bse1I ACTGG 1 cut(s) 537
BseDI CCNNGG 5 cut(s) 29, 303, 348, 467, 468
BseGI GGATG 3 cut(s) 180, 258, 768
BseLI CCNNNNNNNGG 4 cut(s) 30, 322, 490, 951
BseMII CTCAG 2 cut(s) 833, 851
BseNI ACTGG 1 cut(s) 537
BseRI GAGGAG 1 cut(s) 372
BseX3I CGGCCG 4 cut(s) 75, 201, 324, 953
BseXI GCAGC 1 cut(s) 799
Bsh1236I CGCG 9 cut(s) 90, 152, 157, 168, 281, 283, 297, 858, 977
Bsh1285I CGRYCG 4 cut(s) 78, 204, 327, 956
BshFI GGCC 8 cut(s) 77, 101, 203, 221, 326, 363, 955, 969
BshNI GGYRCC 1 cut(s) 1027
BsiEI CGRYCG 4 cut(s) 78, 204, 327, 956
BsiHKCI CYCGRG 1 cut(s) 467
BslFI GGGAC 3 cut(s) 18, 284, 1052
BslI CCNNNNNNNGG 4 cut(s) 30, 322, 490, 951
BsmAI GTCTC 3 cut(s) 703, 941, 983
BsmBI CGTCTC 1 cut(s) 703
BsmFI GGGAC 3 cut(s) 18, 284, 1052
BsnI GGCC 8 cut(s) 77, 101, 203, 221, 326, 363, 955, 969
Bso31I GGTCTC 2 cut(s) 941, 983
BsoBI CYCGRG 1 cut(s) 467
Bsp119I TTCGAA 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 36
Bsp143I GATC 5 cut(s) 129, 525, 853, 1009, 1014
Bsp1720I GCTNAGC 1 cut(s) 842
Bsp68I TCGCGA 2 cut(s) 858, 977
BspACI CCGC 9 cut(s) 88, 204, 228, 231, 267, 809, 846, 898, 1046
BspANI GGCC 8 cut(s) 77, 101, 203, 221, 326, 363, 955, 969
BspCNI CTCAG 2 cut(s) 834, 850
BspFNI CGCG 9 cut(s) 90, 152, 157, 168, 281, 283, 297, 858, 977
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 4 cut(s) 23, 309, 561, 1029
BspMAI CTGCAG 1 cut(s) 644
BspMI ACCTGC 2 cut(s) 65, 125
BspPI GGATC 5 cut(s) 124, 520, 861, 1017, 1022
BspT104I TTCGAA 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 1027
BspTNI GGTCTC 2 cut(s) 941, 983
BsrBI CCGCTC 1 cut(s) 846
BsrFI RCCGGY 3 cut(s) 14, 800, 969
BsrI ACTGG 1 cut(s) 537
BssAI RCCGGY 3 cut(s) 14, 800, 969
BssECI CCNNGG 5 cut(s) 29, 303, 348, 467, 468
BssMI GATC 5 cut(s) 129, 525, 853, 1009, 1014
BssNI GRCGYC 6 cut(s) 225, 252, 372, 399, 1028, 1062
BssSI CACGAG 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 290
Bst6I CTCTTC 2 cut(s) 661, 881
BstACI GRCGYC 6 cut(s) 225, 252, 372, 399, 1028, 1062
BstBAI YACGTR 2 cut(s) 213, 246
BstBI TTCGAA 1 cut(s) 504
BstC8I GCNNGC 5 cut(s) 150, 191, 195, 361, 802
BstDEI CTNAG 3 cut(s) 712, 837, 842
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 3 cut(s) 180, 258, 768
BstFNI CGCG 9 cut(s) 90, 152, 157, 168, 281, 283, 297, 858, 977
BstH2I RGCGCY 1 cut(s) 1031
BstHHI GCGC 7 cut(s) 92, 140, 159, 168, 283, 297, 1030
BstKTI GATC 5 cut(s) 132, 528, 856, 1012, 1017
BstMAI GTCTC 3 cut(s) 703, 941, 983
BstMBI GATC 5 cut(s) 129, 525, 853, 1009, 1014
BstMCI CGRYCG 4 cut(s) 78, 204, 327, 956
BstSCI CCNGG 6 cut(s) 28, 466, 467, 483, 929, 950
BstSFI CTRYAG 1 cut(s) 640
BstUI CGCG 9 cut(s) 90, 152, 157, 168, 281, 283, 297, 858, 977
BstV1I GCAGC 1 cut(s) 799
BstV2I GAAGAC 3 cut(s) 73, 402, 917
BstX2I RGATCY 2 cut(s) 129, 1014
BstYI RGATCY 2 cut(s) 129, 1014
BstZI CGGCCG 4 cut(s) 75, 201, 324, 953
BsuRI GGCC 8 cut(s) 77, 101, 203, 221, 326, 363, 955, 969
BtgI CCRYGG 1 cut(s) 303
BtsCI GGATG 3 cut(s) 180, 258, 768
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
BtuMI TCGCGA 2 cut(s) 858, 977
BveI ACCTGC 2 cut(s) 65, 125
Cac8I GCNNGC 5 cut(s) 150, 191, 195, 361, 802
CciI TCATGA 1 cut(s) 613
CfoI GCGC 7 cut(s) 92, 140, 159, 168, 283, 297, 1030
Cfr10I RCCGGY 3 cut(s) 14, 800, 969
Cfr13I GGNCC 1 cut(s) 21
Cfr9I CCCGGG 1 cut(s) 467
Csp6I GTAC 2 cut(s) 877, 1052
CviAII CATG 1 cut(s) 614
CviQI GTAC 2 cut(s) 877, 1052
DdeI CTNAG 3 cut(s) 712, 837, 842
DinI GGCGCC 1 cut(s) 1029
DpnI GATC 5 cut(s) 131, 527, 855, 1011, 1016
DpnII GATC 5 cut(s) 129, 525, 853, 1009, 1014
EaeI YGGCCR 4 cut(s) 75, 201, 324, 953
EagI CGGCCG 4 cut(s) 75, 201, 324, 953
Eam1104I CTCTTC 2 cut(s) 661, 881
EarI CTCTTC 2 cut(s) 661, 881
EciI GGCGGA 1 cut(s) 256
EclXI CGGCCG 4 cut(s) 75, 201, 324, 953
Eco24I GRGCYC 1 cut(s) 36
Eco31I GGTCTC 2 cut(s) 941, 983
Eco47I GGWCC 1 cut(s) 21
Eco52I CGGCCG 4 cut(s) 75, 201, 324, 953
Eco57I CTGAAG 1 cut(s) 649
Eco72I CACGTG 2 cut(s) 213, 246
Eco88I CYCGRG 1 cut(s) 467
EcoT38I GRGCYC 1 cut(s) 36
EgeI GGCGCC 1 cut(s) 1029
EheI GGCGCC 1 cut(s) 1029
Esp3I CGTCTC 1 cut(s) 703
FaeI CATG 1 cut(s) 617
FaiI YATR 6 cut(s) 476, 548, 615, 760, 942, 1041
FaqI GGGAC 3 cut(s) 18, 284, 1052
FatI CATG 1 cut(s) 613
Fnu4HI GCNGC 6 cut(s) 88, 204, 228, 231, 810, 813
FokI GGATG 3 cut(s) 187, 245, 755
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 6 cut(s) 88, 204, 228, 231, 810, 813
FspBI CTAG 1 cut(s) 317
GlaI GCGC 7 cut(s) 91, 139, 158, 167, 282, 296, 1029
GluI GCNGC 6 cut(s) 88, 204, 228, 231, 810, 813
GsuI CTGGAG 1 cut(s) 520
HaeII RGCGCY 1 cut(s) 1031
HaeIII GGCC 8 cut(s) 77, 101, 203, 221, 326, 363, 955, 969
HhaI GCGC 7 cut(s) 92, 140, 159, 168, 283, 297, 1030
Hin1I GRCGYC 6 cut(s) 225, 252, 372, 399, 1028, 1062
Hin1II CATG 1 cut(s) 617
Hin6I GCGC 7 cut(s) 90, 138, 157, 166, 281, 295, 1028
HinP1I GCGC 7 cut(s) 90, 138, 157, 166, 281, 295, 1028
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 9 cut(s) 95, 122, 320, 329, 404, 494, 580, 835, 960
HphI GGTGA 2 cut(s) 28, 1015
Hpy166II GTNNAC 2 cut(s) 601, 781
Hpy188I TCNGA 3 cut(s) 751, 920, 1014
Hpy188III TCNNGA 8 cut(s) 172, 455, 614, 731, 857, 907, 976, 1069
Hpy8I GTNNAC 2 cut(s) 601, 781
HpyAV CCTTC 1 cut(s) 673
HpyCH4IV ACGT 5 cut(s) 212, 245, 777, 1050, 1073
HpyCH4V TGCA 3 cut(s) 60, 216, 642
HpyF3I CTNAG 3 cut(s) 712, 837, 842
HpySE526I ACGT 5 cut(s) 212, 245, 777, 1050, 1073
Hsp92I GRCGYC 6 cut(s) 225, 252, 372, 399, 1028, 1062
Hsp92II CATG 1 cut(s) 617
HspAI GCGC 7 cut(s) 90, 138, 157, 166, 281, 295, 1028
KasI GGCGCC 1 cut(s) 1027
KroI GCCGGC 1 cut(s) 800
KroNI GCCGGC 1 cut(s) 802
Kzo9I GATC 5 cut(s) 129, 525, 853, 1009, 1014
LmnI GCTCC 4 cut(s) 202, 307, 440, 820
Lsp1109I GCAGC 1 cut(s) 799
LweI GCATC 1 cut(s) 439
MaeI CTAG 1 cut(s) 317
MaeII ACGT 5 cut(s) 212, 245, 777, 1050, 1073
MaeIII GTNAC 2 cut(s) 208, 241
MalI GATC 5 cut(s) 131, 527, 855, 1011, 1016
MbiI CCGCTC 1 cut(s) 846
MboI GATC 5 cut(s) 129, 525, 853, 1009, 1014
MboII GAAGA 7 cut(s) 73, 407, 472, 648, 898, 904, 922
MflI RGATCY 2 cut(s) 129, 1014
MhlI GDGCHC 1 cut(s) 36
MluCI AATT 3 cut(s) 427, 619, 739
Mly113I GGCGCC 1 cut(s) 1028
MlyI GAGTC 5 cut(s) 89, 116, 314, 338, 829
MmeI TCCRAC 1 cut(s) 774
MroNI GCCGGC 1 cut(s) 800
MseI TTAA 2 cut(s) 387, 1020
MspA1I CMGCKG 1 cut(s) 841
MspR9I CCNGG 6 cut(s) 30, 468, 469, 485, 931, 952
MvnI CGCG 9 cut(s) 90, 152, 157, 168, 281, 283, 297, 858, 977
NaeI GCCGGC 1 cut(s) 802
NarI GGCGCC 1 cut(s) 1028
NciI CCSGG 6 cut(s) 30, 468, 469, 485, 931, 952
NdeII GATC 5 cut(s) 129, 525, 853, 1009, 1014
NgoMIV GCCGGC 1 cut(s) 800
NlaIII CATG 1 cut(s) 617
NlaIV GGNNCC 4 cut(s) 23, 309, 561, 1029
NmeAIII GCCGAG 7 cut(s) 77, 302, 329, 352, 970, 981, 1004
NmuCI GTSAC 2 cut(s) 208, 241
NruI TCGCGA 2 cut(s) 858, 977
NspV TTCGAA 1 cut(s) 504
PagI TCATGA 1 cut(s) 613
PaqCI CACCTGC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 830
PdiI GCCGGC 1 cut(s) 802
PfeI GAWTC 4 cut(s) 404, 494, 580, 960
PfoI TCCNGGA 2 cut(s) 483, 929
PkrI GCNGC 6 cut(s) 89, 205, 229, 232, 811, 814
PleI GAGTC 5 cut(s) 89, 116, 314, 337, 829
PluTI GGCGCC 1 cut(s) 1031
PmaCI CACGTG 2 cut(s) 213, 246
PmlI CACGTG 2 cut(s) 213, 246
PpsI GAGTC 5 cut(s) 89, 116, 314, 337, 829
Ppu21I YACGTR 2 cut(s) 213, 246
PspCI CACGTG 2 cut(s) 213, 246
PspN4I GGNNCC 4 cut(s) 23, 309, 561, 1029
PspPI GGNCC 1 cut(s) 21
PstI CTGCAG 1 cut(s) 644
PsuI RGATCY 2 cut(s) 129, 1014
PvuII CAGCTG 1 cut(s) 841
RruI TCGCGA 2 cut(s) 858, 977
RsaI GTAC 2 cut(s) 878, 1053
RsaNI GTAC 2 cut(s) 877, 1052
SaqAI TTAA 2 cut(s) 387, 1020
SatI GCNGC 6 cut(s) 88, 204, 228, 231, 810, 813
Sau3AI GATC 5 cut(s) 129, 525, 853, 1009, 1014
Sau96I GGNCC 1 cut(s) 21
SchI GAGTC 5 cut(s) 89, 116, 314, 338, 829
ScrFI CCNGG 6 cut(s) 30, 468, 469, 485, 931, 952
SduI GDGCHC 1 cut(s) 36
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 640
SfoI GGCGCC 1 cut(s) 1029
SfuI TTCGAA 1 cut(s) 504
SinI GGWCC 1 cut(s) 21
SmaI CCCGGG 1 cut(s) 469
Sse9I AATT 3 cut(s) 427, 619, 739
SsiI CCGC 9 cut(s) 88, 204, 228, 231, 267, 809, 846, 898, 1046
SspDI GGCGCC 1 cut(s) 1027
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 6 cut(s) 28, 466, 467, 483, 929, 950
TaiI ACGT 5 cut(s) 215, 248, 780, 1053, 1076
TaqI TCGA 4 cut(s) 456, 504, 510, 627
TaqII GACCGA 1 cut(s) 1007
TasI AATT 3 cut(s) 427, 619, 739
TauI GCSGC 5 cut(s) 90, 206, 230, 233, 812
TfiI GAWTC 4 cut(s) 404, 494, 580, 960
Tru1I TTAA 2 cut(s) 387, 1020
Tru9I TTAA 2 cut(s) 387, 1020
TscAI CASTG 1 cut(s) 140
TseFI GTSAC 2 cut(s) 208, 241
TseI GCWGC 1 cut(s) 812
Tsp45I GTSAC 2 cut(s) 208, 241
TspDTI ATGAA 5 cut(s) 433, 630, 732, 747, 757
TspGWI ACGGA 3 cut(s) 750, 753, 1013
TspMI CCCGGG 1 cut(s) 467
TspRI CASTG 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 2 cut(s) 427, 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
XmaI CCCGGG 1 cut(s) 467
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.