FvH4_1g02040

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002703
Physical Location & Seq
Forward (+)
1075978 .. 1077386
1409 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02040.t1

Sequence Viewer

Length: 855 bp
ATGGAGGGTCGTGTAGTTGCAATGATATTTTTACTCATGATCATTCTGGTTGCTTCACCTTCACCAAGTTCATGTGCGAGACTTAATGGTTTCCCCACAACAACTACTTTAAGAGATGATGATTCCATTGGTGTTTATGGTGATCAAGTTGCTACTGTAACATCAAATTCATTCAATAATAAGAAATATGGTGATGTTTTTGCAACACCATATAGATTCCATGAGTATCGTCCTCTCTCAAGTAGCCAAACCAGTGATGATTTACAAGCATTTCCGGAAGCAGATGGAACAAGCACATTCAATGTGATGAATTATGGTGCTATTGGGAATGACCAATTTGATAACACACAAGTATGCCTTTCTGAAAGCATGGGAATTGATGTTTGTGGATCCACTCAAGACATCCCTACCCTTATCATACCAAAGGATAACAATTTCCTGCTCAGTTCTGTTGAGTTTAAAGGGCCTTGCAAGGCTGCAAGTGTCAATTTTCAGCTAAATGGGAACATTGTCGCGCCAAATGATACGAATGCTTGGGGAGACAACAAGGGCAGATGGGTTCAGTTTGATCAAGTTCAAGGTCTGATAATCAATGAAGGAGGTCAAATTGATGGTCAAGGTCAGGCTCTCCATTTTGACAGTTGTGATGGTCTTCGGTTGAGTAATATTACGCATATTAATAGTGCAAAAAACCATATAACCATCACCAGCTGCAATGATGTCCAAATTTCTGATATCCTTATCAGGGCTCCAGATGAAAGTCCAAACACTGATGGAATTAACATCTCTACATCAACCAATATCACCATTCAACGTTCTTTTATAGGCACCGGTAACTGGAACCAGATTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

285

Amino Acids

30.8

Weight (kDa)

4.45

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 134 - 278 6.5e-21 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 827
AccII CGCG 1 cut(s) 515
AccIII TCCGGA 1 cut(s) 274
AclI AACGTT 1 cut(s) 814
AclWI GGATC 2 cut(s) 384, 397
AcsI RAATTY 2 cut(s) 166, 726
AfiI CCNNNNNNNGG 2 cut(s) 745, 837
AgeI ACCGGT 1 cut(s) 830
AgsI TTSAA 4 cut(s) 175, 301, 578, 812
AloI GAACNNNNNNTCC 1 cut(s) 833
AluBI AGCT 2 cut(s) 496, 711
AluI AGCT 2 cut(s) 496, 711
Alw26I GTCTC 2 cut(s) 73, 534
AlwI GGATC 2 cut(s) 384, 397
Aor13HI TCCGGA 1 cut(s) 274
AoxI GGCC 1 cut(s) 464
ApeKI GCWGC 2 cut(s) 476, 711
ApoI RAATTY 2 cut(s) 166, 726
ArsI GACNNNNNNTTYG 2 cut(s) 598, 630
AseI ATTAAT 1 cut(s) 678
AsiGI ACCGGT 1 cut(s) 830
AspLEI GCGC 1 cut(s) 517
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 6 cut(s) 48, 54, 152, 203, 697, 796
BamHI GGATCC 1 cut(s) 389
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 1 cut(s) 751
BbsI GAAGAC 1 cut(s) 644
BbvI GCAGC 2 cut(s) 463, 698
BccI CCATC 6 cut(s) 278, 549, 605, 641, 710, 767
BclI TGATCA 3 cut(s) 39, 142, 568
BcoDI GTCTC 2 cut(s) 73, 534
BfaI CTAG 1 cut(s) 853
BisI GCNGC 2 cut(s) 477, 712
BlsI GCNGC 2 cut(s) 478, 713
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 4 cut(s) 391, 750, 829, 842
BpiI GAAGAC 1 cut(s) 644
BpmI CTGGAG 1 cut(s) 735
BpuEI CTTGAG 2 cut(s) 223, 381
BsaWI WCCGGW 2 cut(s) 274, 830
Bsc4I CCNNNNNNNGG 2 cut(s) 745, 837
Bse118I RCCGGY 1 cut(s) 830
Bse1I ACTGG 2 cut(s) 252, 842
Bse3DI GCAATG 2 cut(s) 27, 721
BseAI TCCGGA 1 cut(s) 274
BseGI GGATG 1 cut(s) 402
BseLI CCNNNNNNNGG 2 cut(s) 745, 837
BseMI GCAATG 2 cut(s) 27, 721
BseMII CTCAG 1 cut(s) 457
BseNI ACTGG 2 cut(s) 252, 842
BseXI GCAGC 2 cut(s) 463, 698
Bsh1236I CGCG 1 cut(s) 515
BshFI GGCC 1 cut(s) 466
BshNI GGYRCC 1 cut(s) 827
BshTI ACCGGT 1 cut(s) 830
BsiSI CCGG 2 cut(s) 275, 831
BslI CCNNNNNNNGG 2 cut(s) 745, 837
BsmAI GTCTC 2 cut(s) 73, 534
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 751
Bsp13I TCCGGA 1 cut(s) 274
Bsp143I GATC 4 cut(s) 39, 142, 389, 568
BspANI GGCC 1 cut(s) 466
BspCNI CTCAG 1 cut(s) 456
BspEI TCCGGA 1 cut(s) 274
BspFNI CGCG 1 cut(s) 515
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 4 cut(s) 391, 750, 829, 842
BspPI GGATC 2 cut(s) 384, 397
BspT107I GGYRCC 1 cut(s) 827
BsrDI GCAATG 2 cut(s) 27, 721
BsrFI RCCGGY 1 cut(s) 830
BsrI ACTGG 2 cut(s) 252, 842
BssAI RCCGGY 1 cut(s) 830
BssMI GATC 4 cut(s) 39, 142, 389, 568
Bst4CI ACNGT 2 cut(s) 157, 641
BstDEI CTNAG 1 cut(s) 443
BstF5I GGATG 1 cut(s) 402
BstFNI CGCG 1 cut(s) 515
BstHHI GCGC 1 cut(s) 517
BstKTI GATC 4 cut(s) 42, 145, 392, 571
BstMAI GTCTC 2 cut(s) 73, 534
BstMBI GATC 4 cut(s) 39, 142, 389, 568
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 2 cut(s) 463, 698
BstV2I GAAGAC 1 cut(s) 644
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
BsuRI GGCC 1 cut(s) 466
BtsCI GGATG 1 cut(s) 402
BtsIMutI CAGTG 2 cut(s) 259, 768
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 517
Cfr10I RCCGGY 1 cut(s) 830
Cfr13I GGNCC 1 cut(s) 464
CspAI ACCGGT 1 cut(s) 830
CviAII CATG 4 cut(s) 37, 72, 221, 370
CviJI RGCY 7 cut(s) 246, 466, 476, 496, 626, 711, 749
CviKI_1 RGCY 7 cut(s) 246, 466, 476, 496, 626, 711, 749
DdeI CTNAG 1 cut(s) 443
DpnI GATC 4 cut(s) 41, 144, 391, 570
DpnII GATC 4 cut(s) 39, 142, 389, 568
DraI TTTAAA 1 cut(s) 460
Eco24I GRGCYC 1 cut(s) 751
Eco32I GATATC 1 cut(s) 736
EcoO109I RGGNCCY 1 cut(s) 464
EcoRV GATATC 1 cut(s) 736
EcoT38I GRGCYC 1 cut(s) 751
FaeI CATG 4 cut(s) 40, 75, 224, 373
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FatI CATG 4 cut(s) 36, 71, 220, 369
FbaI TGATCA 3 cut(s) 39, 142, 568
Fnu4HI GCNGC 2 cut(s) 477, 712
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 751
Fsp4HI GCNGC 2 cut(s) 477, 712
FspBI CTAG 1 cut(s) 853
GlaI GCGC 1 cut(s) 516
GluI GCNGC 2 cut(s) 477, 712
GsuI CTGGAG 1 cut(s) 735
HaeIII GGCC 1 cut(s) 466
HapII CCGG 2 cut(s) 275, 831
HhaI GCGC 1 cut(s) 517
Hin1II CATG 4 cut(s) 40, 75, 224, 373
Hin6I GCGC 1 cut(s) 515
HinP1I GCGC 1 cut(s) 515
HinfI GANTC 2 cut(s) 122, 216
HpaII CCGG 2 cut(s) 275, 831
HphI GGTGA 6 cut(s) 48, 54, 152, 203, 697, 796
Hpy188I TCNGA 3 cut(s) 364, 585, 733
Hpy188III TCNNGA 4 cut(s) 37, 275, 398, 752
HpyAV CCTTC 2 cut(s) 69, 590
HpyCH4III ACNGT 2 cut(s) 157, 641
HpyCH4IV ACGT 1 cut(s) 814
HpyCH4V TGCA 6 cut(s) 20, 203, 471, 479, 686, 714
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 1 cut(s) 814
Hsp92II CATG 4 cut(s) 40, 75, 224, 373
HspAI GCGC 1 cut(s) 515
Kpn2I TCCGGA 1 cut(s) 274
Ksp22I TGATCA 3 cut(s) 39, 142, 568
Kzo9I GATC 4 cut(s) 39, 142, 389, 568
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 2 cut(s) 463, 698
MaeI CTAG 1 cut(s) 853
MaeII ACGT 1 cut(s) 814
MaeIII GTNAC 2 cut(s) 157, 833
MalI GATC 4 cut(s) 41, 144, 391, 570
MboI GATC 4 cut(s) 39, 142, 389, 568
MboII GAAGA 1 cut(s) 644
MflI RGATCY 1 cut(s) 389
MhlI GDGCHC 1 cut(s) 751
MluCI AATT 9 cut(s) 166, 310, 335, 375, 433, 487, 606, 726, 777
MnlI CCTC 2 cut(s) 243, 593
MroI TCCGGA 1 cut(s) 274
MseI TTAA 5 cut(s) 84, 110, 459, 678, 780
MslI CAYNNNNRTG 1 cut(s) 352
MspA1I CMGCKG 1 cut(s) 711
MspI CCGG 2 cut(s) 275, 831
Mva1269I GAATGC 1 cut(s) 535
MvnI CGCG 1 cut(s) 515
NdeII GATC 4 cut(s) 39, 142, 389, 568
NlaIII CATG 4 cut(s) 40, 75, 224, 373
NlaIV GGNNCC 4 cut(s) 391, 750, 829, 842
PagI TCATGA 1 cut(s) 36
PctI GAATGC 1 cut(s) 535
PfeI GAWTC 2 cut(s) 122, 216
PinAI ACCGGT 1 cut(s) 830
PkrI GCNGC 2 cut(s) 478, 713
PshBI ATTAAT 1 cut(s) 678
Psp1406I AACGTT 1 cut(s) 814
PspN4I GGNNCC 4 cut(s) 391, 750, 829, 842
PspPI GGNCC 1 cut(s) 464
PsuI RGATCY 1 cut(s) 389
PvuII CAGCTG 1 cut(s) 711
RseI CAYNNNNRTG 1 cut(s) 352
SaqAI TTAA 5 cut(s) 84, 110, 459, 678, 780
SatI GCNGC 2 cut(s) 477, 712
Sau3AI GATC 4 cut(s) 39, 142, 389, 568
Sau96I GGNCC 1 cut(s) 464
SduI GDGCHC 1 cut(s) 751
SetI ASST 7 cut(s) 61, 498, 583, 604, 622, 713, 817
SmiMI CAYNNNNRTG 1 cut(s) 352
SmlI CTYRAG 2 cut(s) 238, 396
SmoI CTYRAG 2 cut(s) 238, 396
Sse9I AATT 9 cut(s) 166, 310, 335, 375, 433, 487, 606, 726, 777
SspI AATATT 1 cut(s) 667
SspMI CTAG 1 cut(s) 853
TaaI ACNGT 2 cut(s) 157, 641
TaiI ACGT 1 cut(s) 817
TasI AATT 9 cut(s) 166, 310, 335, 375, 433, 487, 606, 726, 777
TfiI GAWTC 2 cut(s) 122, 216
Tru1I TTAA 5 cut(s) 84, 110, 459, 678, 780
Tru9I TTAA 5 cut(s) 84, 110, 459, 678, 780
TscAI CASTG 2 cut(s) 259, 775
TseI GCWGC 2 cut(s) 476, 711
TspDTI ATGAA 5 cut(s) 60, 159, 323, 609, 771
TspRI CASTG 2 cut(s) 259, 775
VspI ATTAAT 1 cut(s) 678
XapI RAATTY 2 cut(s) 166, 726
XspI CTAG 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.