Rroxscaffold_4G00320740

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002703
Physical Location & Seq
Forward (+)
49681684 .. 49684041
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320740.1

Sequence Viewer

Length: 1116 bp
ATGGAGGTTCGAATACTTGCTACGATGTTTTTGTTCATGATCATTATGGTTGCTTCACCTTCACCAAGTTCATGTGCTAGACTTAATGGTTTTCCCACAACATATACTTTAAGAGACGATGGTGCCATTGGTTATTATGGTGATCAAGTTGCTGCTGCGACATCAAATCCATTCCATAATAAGGAGTATGGTGATGCTTTTGCAACCCCAAATACATTCGAAGAGTATTATCGTCCTCTTGCAAATCGCCAAACCGATGATGATTTACAAGCATCCCCAGATGCATGGAGATCACTTGCAAAAAGCGAATTCAATGTGATGAATTATGGCGCTGTTGGGAATGGCCAAGTTGATGATACACAAGCTCTCTATATTTACAAGTGTGATGGTCTCCGCTTGAGTGATATTACACATGTTAATAGTCCGAGAAACCATATATTCATCATGGACAGCAATGACGTTCAAATTTCTGATATCCTGATTAGGGCTCCTGATGAAAGTCCAAACACTGATGGAATTAAACTCTCTAGATCAACCAATATTAACATTCACAATTCTTTTATAGGCACTGGTGACGATTGTATTGCAATCATTTCTGGTTCATCTCATATCAACATTACTGATGTTATGTGTGGTCCAGGCCATGGTATAAGTGTTGGAAGTCTTGGTAAGCAAGGTGCTTATAACACAGTTGAAGATGTGCAAGTGAGAAACTGCACCCTCAAGGGAACACAAAATGGAGCAAGAATCAAGACATGGCAAGGCGGGTCAGGGTATGCTAGGAACATCAGTTTTGAGGATATCATAATTCAAGCAGTCAAGAACCCCATCATTATAGACCAGAAGTATATTGATAAGGGCATTACTGGAACTGGAACCTTGCTTGACCAAGGAAGTGCTGTAAAAGTGAGCGATGTGACATTCCGTAACATTCGGGGATCTGTTGCTGGGGAAACAGCCATTACTTTGGACTGTGACGATCAAATTGGTTGCAAAAACATTGTAATGGATAACATAGACTTAACTTCATCTGATCCTGGCAAGAAGGTTTCTGCCCAGTGCAAGAACGTTCAAGGATTTTCTACTTCATCATCTCCTAGTGTGCCATGCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

371

Amino Acids

40.04

Weight (kDa)

4.97

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 118 - 358 1.8e-75 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 644
AciI CCGC 2 cut(s) 394, 765
AclI AACGTT 1 cut(s) 1068
AclWI GGATC 2 cut(s) 946, 1028
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 2 cut(s) 308, 465
AfiI CCNNNNNNNGG 3 cut(s) 181, 484, 644
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 5 cut(s) 313, 464, 695, 812, 1073
AjnI CCWGG 2 cut(s) 637, 1036
AluBI AGCT 1 cut(s) 365
AluI AGCT 1 cut(s) 365
Alw26I GTCTC 2 cut(s) 108, 395
AlwI GGATC 2 cut(s) 946, 1028
AoxI GGCC 2 cut(s) 343, 640
ApeKI GCWGC 2 cut(s) 152, 155
ApoI RAATTY 2 cut(s) 308, 465
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 635
AsuHPI GGTGA 5 cut(s) 48, 54, 152, 203, 584
AsuII TTCGAA 2 cut(s) 10, 219
AvaII GGWCC 1 cut(s) 635
BalI TGGCCA 1 cut(s) 345
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 490
BbvI GCAGC 2 cut(s) 139, 142
BccI CCATC 4 cut(s) 113, 380, 506, 836
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BciT130I CCWGG 2 cut(s) 639, 1038
BclI TGATCA 2 cut(s) 39, 142
BcoDI GTCTC 2 cut(s) 108, 395
BfaI CTAG 4 cut(s) 78, 528, 780, 1098
BfoI RGCGCY 1 cut(s) 333
BisI GCNGC 2 cut(s) 153, 156
BlsI GCNGC 2 cut(s) 154, 157
Bme1390I CCNGG 2 cut(s) 639, 1038
Bme18I GGWCC 1 cut(s) 635
BmgT120I GGNCC 1 cut(s) 635
BmiI GGNNCC 3 cut(s) 124, 489, 877
BmrFI CCNGG 2 cut(s) 639, 1038
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 3 cut(s) 184, 271, 281
BmuI ACTGGG 1 cut(s) 1051
Bpu14I TTCGAA 2 cut(s) 10, 219
BpuEI CTTGAG 2 cut(s) 418, 707
BsaI GGTCTC 1 cut(s) 395
BsaJI CCNNGG 2 cut(s) 643, 889
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 484, 644
Bse1I ACTGG 4 cut(s) 574, 871, 877, 1057
Bse3DI GCAATG 1 cut(s) 460
BseBI CCWGG 2 cut(s) 639, 1038
BseDI CCNNGG 2 cut(s) 643, 889
BseGI GGATG 1 cut(s) 272
BseLI CCNNNNNNNGG 3 cut(s) 181, 484, 644
BseMI GCAATG 1 cut(s) 460
BseNI ACTGG 4 cut(s) 574, 871, 877, 1057
BseXI GCAGC 2 cut(s) 139, 142
BseYI CCCAGC 1 cut(s) 947
BsgI GTGCAG 1 cut(s) 700
BshFI GGCC 2 cut(s) 345, 642
BshNI GGYRCC 1 cut(s) 122
BslI CCNNNNNNNGG 3 cut(s) 181, 484, 644
BsmAI GTCTC 2 cut(s) 108, 395
BsmBI CGTCTC 1 cut(s) 108
BsnI GGCC 2 cut(s) 345, 642
Bso31I GGTCTC 1 cut(s) 395
Bsp119I TTCGAA 2 cut(s) 10, 219
Bsp1286I GDGCHC 1 cut(s) 490
Bsp143I GATC 7 cut(s) 39, 142, 290, 530, 938, 979, 1033
Bsp19I CCATGG 1 cut(s) 643
BspACI CCGC 2 cut(s) 394, 765
BspANI GGCC 2 cut(s) 345, 642
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 3 cut(s) 124, 489, 877
BspPI GGATC 2 cut(s) 946, 1028
BspT104I TTCGAA 2 cut(s) 10, 219
BspT107I GGYRCC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 460
BsrI ACTGG 4 cut(s) 574, 871, 877, 1057
BssECI CCNNGG 2 cut(s) 643, 889
BssMI GATC 7 cut(s) 39, 142, 290, 530, 938, 979, 1033
BssT1I CCWWGG 2 cut(s) 643, 889
Bst2UI CCWGG 2 cut(s) 639, 1038
Bst4CI ACNGT 2 cut(s) 691, 974
Bst6I CTCTTC 1 cut(s) 216
BstBI TTCGAA 2 cut(s) 10, 219
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 1 cut(s) 272
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 7 cut(s) 42, 145, 293, 533, 941, 982, 1036
BstMAI GTCTC 2 cut(s) 108, 395
BstMBI GATC 7 cut(s) 39, 142, 290, 530, 938, 979, 1033
BstNI CCWGG 2 cut(s) 639, 1038
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 637, 1036
BstV1I GCAGC 2 cut(s) 139, 142
BstX2I RGATCY 1 cut(s) 938
BstXI CCANNNNNNTGG 2 cut(s) 285, 967
BstYI RGATCY 1 cut(s) 938
BsuRI GGCC 2 cut(s) 345, 642
BtgI CCRYGG 1 cut(s) 643
BtgZI GCGATG 1 cut(s) 927
BtsCI GGATG 1 cut(s) 272
BtsIMutI CAGTG 3 cut(s) 507, 567, 1064
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 1 cut(s) 635
CviAII CATG 8 cut(s) 37, 72, 285, 413, 445, 644, 756, 1107
CviJI RGCY 5 cut(s) 345, 365, 488, 642, 959
CviKI_1 RGCY 5 cut(s) 345, 365, 488, 642, 959
DpnI GATC 7 cut(s) 41, 144, 292, 532, 940, 981, 1035
DpnII GATC 7 cut(s) 39, 142, 290, 530, 938, 979, 1033
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 643, 889
Eco24I GRGCYC 1 cut(s) 490
Eco31I GGTCTC 1 cut(s) 395
Eco32I GATATC 2 cut(s) 475, 802
Eco47I GGWCC 1 cut(s) 635
EcoRI GAATTC 1 cut(s) 308
EcoRII CCWGG 2 cut(s) 637, 1036
EcoRV GATATC 2 cut(s) 475, 802
EcoT14I CCWWGG 2 cut(s) 643, 889
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 2 cut(s) 643, 889
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 8 cut(s) 40, 75, 288, 416, 448, 647, 759, 1110
FatI CATG 8 cut(s) 36, 71, 284, 412, 444, 643, 755, 1106
FauI CCCGC 1 cut(s) 758
FbaI TGATCA 2 cut(s) 39, 142
Fnu4HI GCNGC 2 cut(s) 153, 156
FokI GGATG 1 cut(s) 259
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 2 cut(s) 153, 156
FspBI CTAG 4 cut(s) 78, 528, 780, 1098
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 153, 156
GsaI CCCAGC 1 cut(s) 951
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 2 cut(s) 345, 642
HhaI GCGC 1 cut(s) 332
Hin1II CATG 8 cut(s) 40, 75, 288, 416, 448, 647, 759, 1110
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 1 cut(s) 747
HphI GGTGA 5 cut(s) 48, 54, 152, 203, 584
Hpy188I TCNGA 3 cut(s) 426, 472, 1033
Hpy188III TCNNGA 6 cut(s) 37, 478, 491, 528, 751, 820
HpyAV CCTTC 2 cut(s) 69, 1039
HpyCH4III ACNGT 2 cut(s) 691, 974
HpyCH4IV ACGT 2 cut(s) 459, 1068
HpyCH4V TGCA 9 cut(s) 203, 242, 284, 299, 587, 703, 717, 993, 1062
HpySE526I ACGT 2 cut(s) 459, 1068
Hsp92II CATG 8 cut(s) 40, 75, 288, 416, 448, 647, 759, 1110
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 2 cut(s) 39, 142
Kzo9I GATC 7 cut(s) 39, 142, 290, 530, 938, 979, 1033
LmnI GCTCC 2 cut(s) 493, 740
Lsp1109I GCAGC 2 cut(s) 139, 142
LweI GCATC 3 cut(s) 184, 271, 281
MaeI CTAG 4 cut(s) 78, 528, 780, 1098
MaeII ACGT 2 cut(s) 459, 1068
MaeIII GTNAC 4 cut(s) 572, 916, 926, 974
MalI GATC 7 cut(s) 41, 144, 292, 532, 940, 981, 1035
MboI GATC 7 cut(s) 39, 142, 290, 530, 938, 979, 1033
MboII GAAGA 2 cut(s) 233, 707
MflI RGATCY 1 cut(s) 938
MhlI GDGCHC 1 cut(s) 490
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 7 cut(s) 308, 322, 465, 516, 553, 807, 984
MluNI TGGCCA 1 cut(s) 345
MmeI TCCRAC 1 cut(s) 637
MnlI CCTC 3 cut(s) 246, 731, 790
Mox20I TGGCCA 1 cut(s) 345
Mph1103I ATGCAT 1 cut(s) 286
MscI TGGCCA 1 cut(s) 345
MseI TTAA 7 cut(s) 84, 110, 417, 519, 543, 1022, 1114
MslI CAYNNNNRTG 1 cut(s) 1004
Msp20I TGGCCA 1 cut(s) 345
MspR9I CCNGG 2 cut(s) 639, 1038
MvaI CCWGG 2 cut(s) 639, 1038
NcoI CCATGG 1 cut(s) 643
NdeII GATC 7 cut(s) 39, 142, 290, 530, 938, 979, 1033
NlaIII CATG 8 cut(s) 40, 75, 288, 416, 448, 647, 759, 1110
NlaIV GGNNCC 3 cut(s) 124, 489, 877
NmuCI GTSAC 3 cut(s) 572, 916, 974
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 416
NspV TTCGAA 2 cut(s) 10, 219
PagI TCATGA 1 cut(s) 36
PciI ACATGT 1 cut(s) 412
PfeI GAWTC 1 cut(s) 747
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 2 cut(s) 154, 157
PscI ACATGT 1 cut(s) 412
PsiI TTATAA 1 cut(s) 684
Psp1406I AACGTT 1 cut(s) 1068
Psp6I CCWGG 2 cut(s) 637, 1036
PspFI CCCAGC 1 cut(s) 947
PspGI CCWGG 2 cut(s) 637, 1036
PspN4I GGNNCC 3 cut(s) 124, 489, 877
PspPI GGNCC 1 cut(s) 635
PsuI RGATCY 1 cut(s) 938
RseI CAYNNNNRTG 1 cut(s) 1004
SaqAI TTAA 7 cut(s) 84, 110, 417, 519, 543, 1022, 1114
SatI GCNGC 2 cut(s) 153, 156
Sau3AI GATC 7 cut(s) 39, 142, 290, 530, 938, 979, 1033
Sau96I GGNCC 1 cut(s) 635
ScrFI CCNGG 2 cut(s) 639, 1038
SduI GDGCHC 1 cut(s) 490
SetI ASST 8 cut(s) 9, 61, 367, 462, 679, 881, 1050, 1071
SfaNI GCATC 3 cut(s) 184, 271, 281
SfuI TTCGAA 2 cut(s) 10, 219
SinI GGWCC 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 1004
SmlI CTYRAG 2 cut(s) 397, 722
SmoI CTYRAG 2 cut(s) 397, 722
Sse9I AATT 7 cut(s) 308, 322, 465, 516, 553, 807, 984
SsiI CCGC 2 cut(s) 394, 765
SspI AATATT 1 cut(s) 541
SspMI CTAG 4 cut(s) 78, 528, 780, 1098
StyD4I CCNGG 2 cut(s) 637, 1036
StyI CCWWGG 2 cut(s) 643, 889
TaaI ACNGT 2 cut(s) 691, 974
TaiI ACGT 2 cut(s) 462, 1071
TaqI TCGA 2 cut(s) 10, 219
TasI AATT 7 cut(s) 308, 322, 465, 516, 553, 807, 984
TfiI GAWTC 1 cut(s) 747
Tru1I TTAA 7 cut(s) 84, 110, 417, 519, 543, 1022, 1114
Tru9I TTAA 7 cut(s) 84, 110, 417, 519, 543, 1022, 1114
TscAI CASTG 3 cut(s) 514, 574, 1064
TseFI GTSAC 3 cut(s) 572, 916, 974
TseI GCWGC 2 cut(s) 152, 155
Tsp45I GTSAC 3 cut(s) 572, 916, 974
TspDTI ATGAA 8 cut(s) 25, 60, 335, 430, 510, 591, 1017, 1077
TspGWI ACGGA 1 cut(s) 914
TspRI CASTG 3 cut(s) 514, 574, 1064
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 2 cut(s) 308, 465
XbaI TCTAGA 1 cut(s) 527
XceI RCATGY 1 cut(s) 416
XspI CTAG 4 cut(s) 78, 528, 780, 1098
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.