Rroxscaffold_5G00370820

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002703
Physical Location & Seq
Forward (+)
52482210 .. 52483960
1751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00370820.1

Sequence Viewer

Length: 987 bp
ATGAATTTTGGCGCTCGTGGCAATGGCCACCATGATGATTCCCAAGCGTTCCTAAAAGCATGGGAGGCTTTATGTGGATCAAGTGAGGAAAGAACCTCTTCCCTGCTAATTCCAAAGGAAAGAACATTCTTGTTAAAACCTTTGGTTTTTAAAGGGCCTTGCAAGCCTGAAACCCTTCATTTCCAGCTCCAAGGGAGACTAGTTGCACCAGATTATAACAGTAAAGCATGGGAGTCAGACAAGGAAAAATGGGTTCAGTTCTCAGATGTAGATGGTCTCATAATCATTGGAGGAGGTCTAGGTGTAATTGATGGTAAGGGTTCCAATTGGTGGAAAGCTTGCGACAAGTCAGACTGCGATCGACCAGCGGCTCTTGAAATTCGTAAATGTGATAATCTCCGACTGAGTGGTGTGACACATATTAATAGCGCAAAAGCCCATATAAAATTCTATGGGTGCAACGGAGTTCAAGTTTCTGGTCTGCTTATTAGCGCTCCTGATGAAAGTCCGAACACAGATGGGATTCAGATCATTTCATCAACTAATGTTAACATTGACAATTCTGCTATAGAAACTGGTGATGACTGTATTGCCATCAGTAGTGGTTCTTCTCAGATCCAAGTTAGTGGTGTTCGATGTGGACCTGGCCATGGTATAAGCATTGGAAGTCTTGGGGAAAATGGAGCTTACGCCACAGTGGAAGACGTGAACCACTACGTCGATAAAGGCCTGTCCGGGCCAAAAGAAGCTGACCGGAAAAGCTCAGTACAAGTGAGTAATGTGACGTACCATGGAGTGTTTGGATCTTCTGCCAATGAGGTAGCTATCAATTTGAACTGCGCTGGAGGGAGGCGCGGTTGCGACAACATTACAATGGATAACATTCACATTACTTCATCCCTTCCCGGGCAGAAGACTTCTGCCTACTGCAACAATGCCCATGGTACAGCTACACTTGCTATCCCTAATGTCCCATGCCTCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

328

Amino Acids

34.8

Weight (kDa)

5.92

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 30 - 237 1.4e-54 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 252 - 316 3.1e-08 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
AccB7I CCANNNNNTGG 1 cut(s) 330
AccII CGCG 1 cut(s) 855
AciI CCGC 2 cut(s) 368, 855
AclWI GGATC 3 cut(s) 85, 610, 811
AcoI YGGCCR 2 cut(s) 25, 646
AcsI RAATTY 3 cut(s) 4, 378, 446
AfaI GTAC 3 cut(s) 768, 788, 946
AfeI AGCGCT 1 cut(s) 493
AfiI CCNNNNNNNGG 3 cut(s) 330, 650, 906
AgsI TTSAA 3 cut(s) 377, 470, 835
AhlI ACTAGT 1 cut(s) 199
AjiI CACGTC 1 cut(s) 706
AjnI CCWGG 1 cut(s) 643
AluBI AGCT 7 cut(s) 187, 338, 686, 749, 762, 824, 950
AluI AGCT 7 cut(s) 187, 338, 686, 749, 762, 824, 950
Alw26I GTCTC 2 cut(s) 190, 281
AlwI GGATC 3 cut(s) 85, 610, 811
Ama87I CYCGRG 1 cut(s) 905
Aor51HI AGCGCT 1 cut(s) 493
AoxI GGCC 5 cut(s) 25, 155, 646, 727, 737
ApoI RAATTY 3 cut(s) 4, 378, 446
AseI ATTAAT 1 cut(s) 423
Asp700I GAANNNNTTC 2 cut(s) 97, 174
AspLEI GCGC 5 cut(s) 14, 431, 494, 842, 855
AspS9I GGNCC 3 cut(s) 155, 641, 737
AsuC2I CCSGG 3 cut(s) 736, 906, 907
AsuHPI GGTGA 1 cut(s) 590
AvaI CYCGRG 1 cut(s) 905
AvaII GGWCC 1 cut(s) 641
BalI TGGCCA 2 cut(s) 27, 648
BarI GAAGNNNNNNTAC 2 cut(s) 592, 624
BauI CACGAG 2 cut(s) 15, 982
BbsI GAAGAC 2 cut(s) 708, 920
BccI CCATC 4 cut(s) 266, 305, 512, 602
BciT130I CCWGG 1 cut(s) 645
BcnI CCSGG 3 cut(s) 736, 906, 907
BcoDI GTCTC 2 cut(s) 190, 281
BcuI ACTAGT 1 cut(s) 199
BfaI CTAG 2 cut(s) 200, 299
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 2 cut(s) 15, 495
BisI GCNGC 1 cut(s) 369
BlsI GCNGC 1 cut(s) 370
Bme1390I CCNGG 4 cut(s) 645, 736, 906, 907
Bme18I GGWCC 1 cut(s) 641
BmeT110I CYCGRG 1 cut(s) 905
BmgBI CACGTC 1 cut(s) 706
BmgT120I GGNCC 3 cut(s) 155, 641, 737
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 4 cut(s) 645, 736, 906, 907
BpiI GAAGAC 2 cut(s) 708, 920
BpmI CTGGAG 1 cut(s) 864
BpuMI CCSGG 3 cut(s) 736, 906, 907
BsaBI GATNNNNATC 1 cut(s) 527
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 5 cut(s) 190, 649, 790, 905, 940
BsaWI WCCGGW 1 cut(s) 753
BsaXI ACNNNNNCTCC 4 cut(s) 456, 486, 841, 871
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 650, 906
Bse1I ACTGG 1 cut(s) 580
Bse3DI GCAATG 1 cut(s) 28
Bse8I GATNNNNATC 1 cut(s) 527
BseBI CCWGG 1 cut(s) 645
BseDI CCNNGG 5 cut(s) 190, 649, 790, 905, 940
BseGI GGATG 1 cut(s) 896
BseJI GATNNNNATC 1 cut(s) 527
BseLI CCNNNNNNNGG 3 cut(s) 330, 650, 906
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 4 cut(s) 276, 395, 626, 777
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 306
Bsh1236I CGCG 1 cut(s) 855
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 5 cut(s) 27, 157, 648, 729, 739
BsiEI CGRYCG 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 905
BsiSI CCGG 3 cut(s) 735, 754, 906
BslFI GGGAC 1 cut(s) 956
BslI CCNNNNNNNGG 3 cut(s) 330, 650, 906
BsmAI GTCTC 2 cut(s) 190, 281
BsmFI GGGAC 1 cut(s) 956
BsnI GGCC 5 cut(s) 27, 157, 648, 729, 739
Bso31I GGTCTC 1 cut(s) 281
BsoBI CYCGRG 1 cut(s) 905
Bsp143I GATC 5 cut(s) 77, 358, 528, 615, 803
Bsp19I CCATGG 3 cut(s) 649, 790, 940
BspACI CCGC 2 cut(s) 368, 855
BspANI GGCC 5 cut(s) 27, 157, 648, 729, 739
BspCNI CTCAG 4 cut(s) 275, 396, 625, 776
BspFNI CGCG 1 cut(s) 855
BspLI GGNNCC 1 cut(s) 322
BspPI GGATC 3 cut(s) 85, 610, 811
BspTNI GGTCTC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 28
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 5 cut(s) 190, 649, 790, 905, 940
BssMI GATC 5 cut(s) 77, 358, 528, 615, 803
BssSI CACGAG 2 cut(s) 15, 982
BssT1I CCWWGG 4 cut(s) 190, 649, 790, 940
Bst2BI CACGAG 2 cut(s) 15, 982
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 3 cut(s) 221, 587, 697
Bst6I CTCTTC 1 cut(s) 103
BstC8I GCNNGC 2 cut(s) 164, 340
BstDEI CTNAG 4 cut(s) 262, 404, 612, 763
BstDSI CCRYGG 3 cut(s) 649, 790, 940
BstF5I GGATG 1 cut(s) 896
BstFNI CGCG 1 cut(s) 855
BstH2I RGCGCY 2 cut(s) 15, 495
BstHHI GCGC 5 cut(s) 14, 431, 494, 842, 855
BstKTI GATC 5 cut(s) 80, 361, 531, 618, 806
BstMAI GTCTC 2 cut(s) 190, 281
BstMBI GATC 5 cut(s) 77, 358, 528, 615, 803
BstMCI CGRYCG 1 cut(s) 361
BstMWI GCNNNNNNNGC 4 cut(s) 18, 65, 163, 956
BstNI CCWGG 1 cut(s) 645
BstSCI CCNGG 4 cut(s) 643, 734, 904, 905
BstSFI CTRYAG 1 cut(s) 567
BstUI CGCG 1 cut(s) 855
BstV2I GAAGAC 2 cut(s) 708, 920
BstX2I RGATCY 2 cut(s) 615, 803
BstXI CCANNNNNNTGG 1 cut(s) 626
BstYI RGATCY 2 cut(s) 615, 803
BsuRI GGCC 5 cut(s) 27, 157, 648, 729, 739
BtgI CCRYGG 3 cut(s) 649, 790, 940
BtrI CACGTC 1 cut(s) 706
BtsCI GGATG 1 cut(s) 896
BtsIMutI CAGTG 1 cut(s) 702
Cac8I GCNNGC 2 cut(s) 164, 340
CfoI GCGC 5 cut(s) 14, 431, 494, 842, 855
Cfr13I GGNCC 3 cut(s) 155, 641, 737
Cfr9I CCCGGG 1 cut(s) 905
Csp6I GTAC 3 cut(s) 767, 787, 945
CviAII CATG 7 cut(s) 32, 60, 228, 650, 791, 941, 975
CviQI GTAC 3 cut(s) 767, 787, 945
DdeI CTNAG 4 cut(s) 262, 404, 612, 763
DpnI GATC 5 cut(s) 79, 360, 530, 617, 805
DpnII GATC 5 cut(s) 77, 358, 528, 615, 803
DraI TTTAAA 1 cut(s) 151
EaeI YGGCCR 2 cut(s) 25, 646
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Eco130I CCWWGG 4 cut(s) 190, 649, 790, 940
Eco147I AGGCCT 1 cut(s) 729
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 641
Eco47III AGCGCT 1 cut(s) 493
Eco88I CYCGRG 1 cut(s) 905
EcoO109I RGGNCCY 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 643
EcoT14I CCWWGG 4 cut(s) 190, 649, 790, 940
ErhI CCWWGG 4 cut(s) 190, 649, 790, 940
FaeI CATG 7 cut(s) 35, 63, 231, 653, 794, 944, 978
FalI AAGNNNNNCTT 2 cut(s) 82, 114
FaqI GGGAC 1 cut(s) 956
FatI CATG 7 cut(s) 31, 59, 227, 649, 790, 940, 974
Fnu4HI GCNGC 1 cut(s) 369
FokI GGATG 1 cut(s) 883
Fsp4HI GCNGC 1 cut(s) 369
FspBI CTAG 2 cut(s) 200, 299
GlaI GCGC 5 cut(s) 13, 430, 493, 841, 854
GluI GCNGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 864
HaeII RGCGCY 2 cut(s) 15, 495
HaeIII GGCC 5 cut(s) 27, 157, 648, 729, 739
HapII CCGG 3 cut(s) 735, 754, 906
HhaI GCGC 5 cut(s) 14, 431, 494, 842, 855
Hin1II CATG 7 cut(s) 35, 63, 231, 653, 794, 944, 978
Hin6I GCGC 5 cut(s) 12, 429, 492, 840, 853
HinP1I GCGC 5 cut(s) 12, 429, 492, 840, 853
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 3 cut(s) 38, 233, 523
HpaI GTTAAC 1 cut(s) 550
HpaII CCGG 3 cut(s) 735, 754, 906
HphI GGTGA 1 cut(s) 590
Hpy166II GTNNAC 3 cut(s) 550, 641, 709
Hpy188I TCNGA 7 cut(s) 238, 265, 352, 401, 510, 528, 615
Hpy188III TCNNGA 3 cut(s) 374, 497, 984
Hpy8I GTNNAC 3 cut(s) 550, 641, 709
Hpy99I CGWCG 1 cut(s) 722
HpyAV CCTTC 2 cut(s) 185, 911
HpyCH4III ACNGT 3 cut(s) 221, 587, 697
HpyCH4IV ACGT 3 cut(s) 705, 717, 785
HpyCH4V TGCA 4 cut(s) 162, 206, 459, 930
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 65, 163, 956
HpyF3I CTNAG 4 cut(s) 262, 404, 612, 763
HpySE526I ACGT 3 cut(s) 705, 717, 785
Hsp92II CATG 7 cut(s) 35, 63, 231, 653, 794, 944, 978
HspAI GCGC 5 cut(s) 12, 429, 492, 840, 853
KspAI GTTAAC 1 cut(s) 550
Kzo9I GATC 5 cut(s) 77, 358, 528, 615, 803
LmnI GCTCC 3 cut(s) 192, 499, 683
MaeI CTAG 2 cut(s) 200, 299
MaeII ACGT 3 cut(s) 705, 717, 785
MaeIII GTNAC 2 cut(s) 412, 781
MalI GATC 5 cut(s) 79, 360, 530, 617, 805
MboI GATC 5 cut(s) 77, 358, 528, 615, 803
MboII GAAGA 5 cut(s) 90, 600, 713, 798, 925
MfeI CAATTG 1 cut(s) 325
MflI RGATCY 2 cut(s) 615, 803
MlsI TGGCCA 2 cut(s) 27, 648
MluCI AATT 8 cut(s) 4, 108, 306, 325, 378, 446, 559, 829
MluNI TGGCCA 2 cut(s) 27, 648
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 8 cut(s) 58, 79, 106, 284, 287, 811, 839, 843
Mox20I TGGCCA 2 cut(s) 27, 648
MroXI GAANNNNTTC 2 cut(s) 97, 174
MscI TGGCCA 2 cut(s) 27, 648
MseI TTAA 4 cut(s) 134, 150, 423, 549
MslI CAYNNNNRTG 2 cut(s) 33, 872
Msp20I TGGCCA 2 cut(s) 27, 648
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 3 cut(s) 735, 754, 906
MspR9I CCNGG 4 cut(s) 645, 736, 906, 907
MunI CAATTG 1 cut(s) 325
MvaI CCWGG 1 cut(s) 645
MvnI CGCG 1 cut(s) 855
MwoI GCNNNNNNNGC 4 cut(s) 18, 65, 163, 956
NciI CCSGG 3 cut(s) 736, 906, 907
NcoI CCATGG 3 cut(s) 649, 790, 940
NdeII GATC 5 cut(s) 77, 358, 528, 615, 803
NlaIII CATG 7 cut(s) 35, 63, 231, 653, 794, 944, 978
NlaIV GGNNCC 1 cut(s) 322
NmuCI GTSAC 2 cut(s) 412, 781
PceI AGGCCT 1 cut(s) 729
PdmI GAANNNNTTC 2 cut(s) 97, 174
PfeI GAWTC 2 cut(s) 38, 523
PflMI CCANNNNNTGG 1 cut(s) 330
PkrI GCNGC 1 cut(s) 370
Ple19I CGATCG 1 cut(s) 361
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PshBI ATTAAT 1 cut(s) 423
PsiI TTATAA 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 643
PspGI CCWGG 1 cut(s) 643
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 3 cut(s) 155, 641, 737
PsuI RGATCY 2 cut(s) 615, 803
PvuI CGATCG 1 cut(s) 361
RsaI GTAC 3 cut(s) 768, 788, 946
RsaNI GTAC 3 cut(s) 767, 787, 945
RseI CAYNNNNRTG 2 cut(s) 33, 872
SaqAI TTAA 4 cut(s) 134, 150, 423, 549
SatI GCNGC 1 cut(s) 369
Sau3AI GATC 5 cut(s) 77, 358, 528, 615, 803
Sau96I GGNCC 3 cut(s) 155, 641, 737
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 4 cut(s) 645, 736, 906, 907
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 1 cut(s) 641
SmaI CCCGGG 1 cut(s) 907
SmiMI CAYNNNNRTG 2 cut(s) 33, 872
SpeI ACTAGT 1 cut(s) 199
Sse9I AATT 8 cut(s) 4, 108, 306, 325, 378, 446, 559, 829
SseBI AGGCCT 1 cut(s) 729
SsiI CCGC 2 cut(s) 368, 855
SspMI CTAG 2 cut(s) 200, 299
StuI AGGCCT 1 cut(s) 729
StyD4I CCNGG 4 cut(s) 643, 734, 904, 905
StyI CCWWGG 4 cut(s) 190, 649, 790, 940
TaaI ACNGT 3 cut(s) 221, 587, 697
TaiI ACGT 3 cut(s) 708, 720, 788
TaqI TCGA 3 cut(s) 361, 634, 720
TasI AATT 8 cut(s) 4, 108, 306, 325, 378, 446, 559, 829
TatI WGTACW 1 cut(s) 766
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 2 cut(s) 38, 523
Tru1I TTAA 4 cut(s) 134, 150, 423, 549
Tru9I TTAA 4 cut(s) 134, 150, 423, 549
TscAI CASTG 1 cut(s) 702
TseFI GTSAC 2 cut(s) 412, 781
Tsp45I GTSAC 2 cut(s) 412, 781
TspDTI ATGAA 5 cut(s) 17, 167, 516, 525, 885
TspGWI ACGGA 1 cut(s) 477
TspMI CCCGGG 1 cut(s) 905
TspRI CASTG 1 cut(s) 702
Van91I CCANNNNNTGG 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 641
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 3 cut(s) 4, 378, 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
XmaI CCCGGG 1 cut(s) 905
XmnI GAANNNNTTC 2 cut(s) 97, 174
XspI CTAG 2 cut(s) 200, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.