FvH4_7g04480

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002703
Physical Location & Seq
Reverse (-)
5025873 .. 5028213
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04480.t1

Sequence Viewer

Length: 1422 bp
ATGGAGGGTCGAGTACTTGCAATGATCTTTTTGTTCATGATCATTCTGGTTGCTTCACCCTCACCAAGTTCATGTGCTAGACTTACAGGTTTTCCCACAACAAATACTTTAAGAGATGATGACGAATTCATTGGTTATTATAGTGATCAAGGTGCTGATAAAACATCAAATTCATTCAAAGAAGAATATGGGGTTGTTGTTGCAACTACATCCGACAAGTATTATCATGCTCTTCCCAATCGCCAAACCGATGATGACTTACAAGCATTCCCAGAAGAAGCATGGAGATCACCTGCAACAAGCACATTCAATGTGATGAATTATGGTGCTGTTGGGAATGGCGAAGTTGATGATACGCAAGCCTTTCTGAAAGCATGGGGAGATGTTTGTGGATCCACTCAAGGCATCCCTACCCTTATCATACCAAAGGGCAAAGATTTCTTGCTCAATTCTGTGAAGTTTCAAGGGCCTTGCAGGGCTGCAAGTGTAAATTTTCAGCTAAATGGGAGCATTGTCGCGAAAAAGGATATGACTGCTTGGAGAGACAAGGACAAATGGGTTCAGTTTGACAATGTTCAAGGTCTGATAATCAATGGAGGAGGTCAAATTGATGGTCAAGGTCTGGTTTGGTGGAGAGCTTGCAGCACTACAAATTGCAAACGACCAACTGCTCTCCATATTAACGACTGTCATGGTCTCCGGTTGAGTGATATTACGCATATTGATAGTGCGAGGAACCATATCAGCATCAGCGGCTGCAATGATGTCGAAGTTTCTGGTATCCTTATTAGGGCTCCAGCAGATAGTCCAAACACTGATGGAATTGACATCTCTACATCAACCAATATCAACATTCGAGATTCTTCTATAGGCACTGGTGACGACTGTATTGCAATCAATTCTGGTACATCTCATGTCAACGTTACTGGTGTTTTGTGCGGTCCAGGCCATGGTATAAGTATCGGAAGTCTTGGTGAGCATGGTTCGTATAGCATGGTGGAAGATGTGCAAGTGAGAAAGTGCACCTTCAATGGCACAACAAATGGAGCAAGAATCAAGACATGGAAAGGCGGATCAGGGTATGCTAGGGACATCATTTTTGAGAATATCACAATTCAAGCAATCAAGAACCCTATCATTATAGACCAGGAGTATTCTGATGTTCTCTATACTGGATTTGGAACCCTGCATGACCAAGGAAGCGCTGTACAAGTGAGCGACGTGACGTTCCGTGACTTTCAGGGATCTGTTTCTGGGGATGTAGCCATTACTTTGGACTGCGACAATCGAGTAGGCTGCAAAAACATTGTAATGGATCACATCGACTTAACTTCGTCCGTTCATGGCAAGAAGGTTGCTGCCTACTGCAAGAACGTCCGAGGATTACGTACTTCATTATCTCCTAGTGTGACATGCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

474

Amino Acids

51.02

Weight (kDa)

5.29

Isoelectric Point (pI)

27.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 134 - 460 3.3e-90 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 301
Acc36I ACCTGC 1 cut(s) 301
AccB7I CCANNNNNTGG 1 cut(s) 950
AccII CGCG 1 cut(s) 518
AciI CCGC 3 cut(s) 753, 939, 1071
AclI AACGTT 1 cut(s) 921
AclWI GGATC 5 cut(s) 387, 400, 1081, 1252, 1323
AcsI RAATTY 3 cut(s) 125, 169, 490
AfaI GTAC 4 cut(s) 15, 907, 1209, 1390
AfeI AGCGCT 1 cut(s) 1204
AfiI CCNNNNNNNGG 2 cut(s) 790, 950
AgsI TTSAA 6 cut(s) 178, 310, 464, 578, 1030, 1118
AjiI CACGTC 1 cut(s) 1222
AjnI CCWGG 2 cut(s) 943, 1146
AluBI AGCT 2 cut(s) 499, 638
AluI AGCT 2 cut(s) 499, 638
Alw21I GWGCWC 1 cut(s) 1025
Alw26I GTCTC 2 cut(s) 537, 701
Alw44I GTGCAC 1 cut(s) 1021
AlwI GGATC 5 cut(s) 387, 400, 1081, 1252, 1323
AlwNI CAGNNNCTG 1 cut(s) 756
Aor51HI AGCGCT 1 cut(s) 1204
AoxI GGCC 2 cut(s) 467, 946
ApaLI GTGCAC 1 cut(s) 1021
ApeKI GCWGC 5 cut(s) 479, 642, 756, 1296, 1358
ApoI RAATTY 3 cut(s) 125, 169, 490
ArsI GACNNNNNNTTYG 4 cut(s) 598, 630, 1082, 1114
AspLEI GCGC 1 cut(s) 1205
AspS9I GGNCC 2 cut(s) 467, 941
AsuHPI GGTGA 5 cut(s) 48, 54, 282, 890, 986
AvaII GGWCC 1 cut(s) 941
BaeGI GKGCMC 1 cut(s) 1025
BamHI GGATCC 1 cut(s) 392
BanII GRGCYC 1 cut(s) 796
Bbv12I GWGCWC 1 cut(s) 1025
BbvI GCAGC 5 cut(s) 466, 654, 743, 1283, 1345
BccI CCATC 2 cut(s) 605, 812
BcgI CGANNNNNNTGC 6 cut(s) 748, 782, 872, 906, 1278, 1312
BciT130I CCWGG 2 cut(s) 945, 1148
BciVI GTATCC 1 cut(s) 791
BclI TGATCA 2 cut(s) 39, 145
BcoDI GTCTC 2 cut(s) 537, 701
BfaI CTAG 3 cut(s) 78, 1086, 1404
BfmI CTRYAG 1 cut(s) 867
BfoI RGCGCY 1 cut(s) 1206
BfuAI ACCTGC 1 cut(s) 301
BfuI GTATCC 1 cut(s) 791
BisI GCNGC 6 cut(s) 480, 643, 754, 757, 1297, 1359
BlsI GCNGC 6 cut(s) 481, 644, 755, 758, 1298, 1360
BmcAI AGTACT 1 cut(s) 15
Bme1390I CCNGG 2 cut(s) 945, 1148
Bme18I GGWCC 1 cut(s) 941
BmgBI CACGTC 1 cut(s) 1222
BmgT120I GGNCC 2 cut(s) 467, 941
BmiI GGNNCC 4 cut(s) 394, 737, 795, 1183
BmrFI CCNGG 2 cut(s) 945, 1148
BmsI GCATC 2 cut(s) 414, 756
BpmI CTGGAG 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 1388
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 3 cut(s) 949, 1195, 1378
BsaWI WCCGGW 1 cut(s) 699
Bsc4I CCNNNNNNNGG 2 cut(s) 790, 950
Bse1I ACTGG 3 cut(s) 880, 931, 1177
Bse3DI GCAATG 2 cut(s) 27, 766
BseBI CCWGG 2 cut(s) 945, 1148
BseDI CCNNGG 3 cut(s) 949, 1195, 1378
BseGI GGATG 3 cut(s) 209, 405, 1264
BseLI CCNNNNNNNGG 2 cut(s) 790, 950
BseMI GCAATG 2 cut(s) 27, 766
BseNI ACTGG 3 cut(s) 880, 931, 1177
BseRI GAGGAG 1 cut(s) 612
BseSI GKGCMC 1 cut(s) 1025
BseXI GCAGC 5 cut(s) 466, 654, 743, 1283, 1345
Bsh1236I CGCG 1 cut(s) 518
BshFI GGCC 2 cut(s) 469, 948
BsiHKAI GWGCWC 1 cut(s) 1025
BsiSI CCGG 1 cut(s) 700
BslFI GGGAC 1 cut(s) 1103
BslI CCNNNNNNNGG 2 cut(s) 790, 950
BsmAI GTCTC 2 cut(s) 537, 701
BsmFI GGGAC 1 cut(s) 1103
BsmI GAATGC 1 cut(s) 266
BsnI GGCC 2 cut(s) 469, 948
Bso31I GGTCTC 1 cut(s) 701
Bsp1286I GDGCHC 2 cut(s) 796, 1025
Bsp1407I TGTACA 1 cut(s) 1207
Bsp143I GATC 8 cut(s) 24, 39, 145, 287, 392, 1073, 1244, 1315
Bsp19I CCATGG 1 cut(s) 949
Bsp68I TCGCGA 1 cut(s) 518
BspACI CCGC 3 cut(s) 753, 939, 1071
BspANI GGCC 2 cut(s) 469, 948
BspFNI CGCG 1 cut(s) 518
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 4 cut(s) 394, 737, 795, 1183
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 5 cut(s) 387, 400, 1081, 1252, 1323
BspQI GCTCTTC 1 cut(s) 237
BspTNI GGTCTC 1 cut(s) 701
BsrDI GCAATG 2 cut(s) 27, 766
BsrGI TGTACA 1 cut(s) 1207
BsrI ACTGG 3 cut(s) 880, 931, 1177
BssECI CCNNGG 3 cut(s) 949, 1195, 1378
BssMI GATC 8 cut(s) 24, 39, 145, 287, 392, 1073, 1244, 1315
BssT1I CCWWGG 2 cut(s) 949, 1195
Bst2UI CCWGG 2 cut(s) 945, 1148
Bst4CI ACNGT 2 cut(s) 689, 887
Bst6I CTCTTC 1 cut(s) 237
BstAUI TGTACA 1 cut(s) 1207
BstBAI YACGTR 1 cut(s) 1388
BstC8I GCNNGC 2 cut(s) 360, 640
BstDSI CCRYGG 1 cut(s) 949
BstF5I GGATG 3 cut(s) 209, 405, 1264
BstFNI CGCG 1 cut(s) 518
BstH2I RGCGCY 1 cut(s) 1206
BstHHI GCGC 1 cut(s) 1205
BstKTI GATC 8 cut(s) 27, 42, 148, 290, 395, 1076, 1247, 1318
BstMAI GTCTC 2 cut(s) 537, 701
BstMBI GATC 8 cut(s) 24, 39, 145, 287, 392, 1073, 1244, 1315
BstMWI GCNNNNNNNGC 2 cut(s) 753, 945
BstNI CCWGG 2 cut(s) 945, 1148
BstNSI RCATGY 1 cut(s) 1416
BstSCI CCNGG 2 cut(s) 943, 1146
BstSFI CTRYAG 1 cut(s) 867
BstSLI GKGCMC 1 cut(s) 1025
BstSNI TACGTA 1 cut(s) 1388
BstUI CGCG 1 cut(s) 518
BstV1I GCAGC 5 cut(s) 466, 654, 743, 1283, 1345
BstX2I RGATCY 2 cut(s) 392, 1244
BstXI CCANNNNNNTGG 1 cut(s) 1273
BstYI RGATCY 2 cut(s) 392, 1244
BsuI GTATCC 1 cut(s) 791
BsuRI GGCC 2 cut(s) 469, 948
BtgI CCRYGG 1 cut(s) 949
BtrI CACGTC 1 cut(s) 1222
BtsCI GGATG 3 cut(s) 209, 405, 1264
BtsIMutI CAGTG 2 cut(s) 813, 873
BtuMI TCGCGA 1 cut(s) 518
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 2 cut(s) 360, 640
CaiI CAGNNNCTG 1 cut(s) 756
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 1205
Cfr13I GGNCC 2 cut(s) 467, 941
Csp6I GTAC 4 cut(s) 14, 906, 1208, 1389
CviQI GTAC 4 cut(s) 14, 906, 1208, 1389
DpnI GATC 8 cut(s) 26, 41, 147, 289, 394, 1075, 1246, 1317
DpnII GATC 8 cut(s) 24, 39, 145, 287, 392, 1073, 1244, 1315
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
EciI GGCGGA 1 cut(s) 1086
Eco105I TACGTA 1 cut(s) 1388
Eco130I CCWWGG 2 cut(s) 949, 1195
Eco24I GRGCYC 1 cut(s) 796
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 1 cut(s) 941
Eco47III AGCGCT 1 cut(s) 1204
EcoO109I RGGNCCY 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 943, 1146
EcoT14I CCWWGG 2 cut(s) 949, 1195
EcoT38I GRGCYC 1 cut(s) 796
ErhI CCWWGG 2 cut(s) 949, 1195
FalI AAGNNNNNCTT 2 cut(s) 1010, 1042
FaqI GGGAC 1 cut(s) 1103
FbaI TGATCA 2 cut(s) 39, 145
Fnu4HI GCNGC 6 cut(s) 480, 643, 754, 757, 1297, 1359
FokI GGATG 3 cut(s) 196, 392, 1271
FriOI GRGCYC 1 cut(s) 796
Fsp4HI GCNGC 6 cut(s) 480, 643, 754, 757, 1297, 1359
FspBI CTAG 3 cut(s) 78, 1086, 1404
GlaI GCGC 1 cut(s) 1204
GluI GCNGC 6 cut(s) 480, 643, 754, 757, 1297, 1359
GsuI CTGGAG 1 cut(s) 780
HaeII RGCGCY 1 cut(s) 1206
HaeIII GGCC 2 cut(s) 469, 948
HapII CCGG 1 cut(s) 700
HhaI GCGC 1 cut(s) 1205
Hin6I GCGC 1 cut(s) 1203
HinP1I GCGC 1 cut(s) 1203
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HinfI GANTC 2 cut(s) 860, 1053
HpaII CCGG 1 cut(s) 700
HphI GGTGA 5 cut(s) 48, 54, 282, 890, 986
Hpy166II GTNNAC 2 cut(s) 919, 1023
Hpy188I TCNGA 6 cut(s) 214, 369, 585, 965, 1159, 1379
Hpy188III TCNNGA 5 cut(s) 37, 517, 857, 1057, 1126
Hpy8I GTNNAC 2 cut(s) 919, 1023
Hpy99I CGWCG 1 cut(s) 1223
HpyAV CCTTC 2 cut(s) 1036, 1345
HpyCH4III ACNGT 2 cut(s) 689, 887
HpyCH4IV ACGT 5 cut(s) 921, 1221, 1226, 1374, 1387
HpyF10VI GCNNNNNNNGC 2 cut(s) 753, 945
HpySE526I ACGT 5 cut(s) 921, 1221, 1226, 1374, 1387
HspAI GCGC 1 cut(s) 1203
Ksp22I TGATCA 2 cut(s) 39, 145
Kzo9I GATC 8 cut(s) 24, 39, 145, 287, 392, 1073, 1244, 1315
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 3 cut(s) 507, 799, 1046
Lsp1109I GCAGC 5 cut(s) 466, 654, 743, 1283, 1345
LweI GCATC 2 cut(s) 414, 756
MaeI CTAG 3 cut(s) 78, 1086, 1404
MaeII ACGT 5 cut(s) 921, 1221, 1226, 1374, 1387
MaeIII GTNAC 5 cut(s) 878, 922, 1222, 1232, 1408
MalI GATC 8 cut(s) 26, 41, 147, 289, 394, 1075, 1246, 1317
MboI GATC 8 cut(s) 24, 39, 145, 287, 392, 1073, 1244, 1315
MboII GAAGA 5 cut(s) 194, 224, 287, 855, 1013
MflI RGATCY 2 cut(s) 392, 1244
MhlI GDGCHC 2 cut(s) 796, 1025
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 5 cut(s) 70, 590, 593, 726, 1373
MseI TTAA 4 cut(s) 110, 681, 1328, 1420
MslI CAYNNNNRTG 1 cut(s) 1310
MspA1I CMGCKG 1 cut(s) 753
MspI CCGG 1 cut(s) 700
MspR9I CCNGG 2 cut(s) 945, 1148
Mva1269I GAATGC 1 cut(s) 266
MvaI CCWGG 2 cut(s) 945, 1148
MvnI CGCG 1 cut(s) 518
MwoI GCNNNNNNNGC 2 cut(s) 753, 945
NcoI CCATGG 1 cut(s) 949
NdeII GATC 8 cut(s) 24, 39, 145, 287, 392, 1073, 1244, 1315
NlaIV GGNNCC 4 cut(s) 394, 737, 795, 1183
NmuCI GTSAC 4 cut(s) 878, 1222, 1232, 1408
NruI TCGCGA 1 cut(s) 518
NspI RCATGY 1 cut(s) 1416
PagI TCATGA 1 cut(s) 36
PaqCI CACCTGC 1 cut(s) 301
PciSI GCTCTTC 1 cut(s) 237
PctI GAATGC 1 cut(s) 266
PfeI GAWTC 2 cut(s) 860, 1053
PflMI CCANNNNNTGG 1 cut(s) 950
PkrI GCNGC 6 cut(s) 481, 644, 755, 758, 1298, 1360
Ppu21I YACGTR 1 cut(s) 1388
Psp1406I AACGTT 1 cut(s) 921
Psp6I CCWGG 2 cut(s) 943, 1146
PspGI CCWGG 2 cut(s) 943, 1146
PspN4I GGNNCC 4 cut(s) 394, 737, 795, 1183
PspPI GGNCC 2 cut(s) 467, 941
PstNI CAGNNNCTG 1 cut(s) 756
PsuI RGATCY 2 cut(s) 392, 1244
RruI TCGCGA 1 cut(s) 518
RsaI GTAC 4 cut(s) 15, 907, 1209, 1390
RsaNI GTAC 4 cut(s) 14, 906, 1208, 1389
RseI CAYNNNNRTG 1 cut(s) 1310
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 4 cut(s) 110, 681, 1328, 1420
SatI GCNGC 6 cut(s) 480, 643, 754, 757, 1297, 1359
Sau3AI GATC 8 cut(s) 24, 39, 145, 287, 392, 1073, 1244, 1315
Sau96I GGNCC 2 cut(s) 467, 941
ScaI AGTACT 1 cut(s) 15
ScrFI CCNGG 2 cut(s) 945, 1148
SduI GDGCHC 2 cut(s) 796, 1025
SfaNI GCATC 2 cut(s) 414, 756
SfcI CTRYAG 1 cut(s) 867
SinI GGWCC 1 cut(s) 941
SmiMI CAYNNNNRTG 1 cut(s) 1310
SmlI CTYRAG 1 cut(s) 399
SmoI CTYRAG 1 cut(s) 399
SnaBI TACGTA 1 cut(s) 1388
SsiI CCGC 3 cut(s) 753, 939, 1071
SspMI CTAG 3 cut(s) 78, 1086, 1404
StyD4I CCNGG 2 cut(s) 943, 1146
StyI CCWWGG 2 cut(s) 949, 1195
TaaI ACNGT 2 cut(s) 689, 887
TaiI ACGT 5 cut(s) 924, 1224, 1229, 1377, 1390
TaqI TCGA 5 cut(s) 10, 768, 856, 1288, 1323
TatI WGTACW 2 cut(s) 13, 1207
TauI GCSGC 1 cut(s) 756
TfiI GAWTC 2 cut(s) 860, 1053
Tru1I TTAA 4 cut(s) 110, 681, 1328, 1420
Tru9I TTAA 4 cut(s) 110, 681, 1328, 1420
TscAI CASTG 2 cut(s) 820, 880
TseFI GTSAC 4 cut(s) 878, 1222, 1232, 1408
TseI GCWGC 5 cut(s) 479, 642, 756, 1296, 1358
Tsp45I GTSAC 4 cut(s) 878, 1222, 1232, 1408
TspDTI ATGAA 7 cut(s) 25, 60, 118, 162, 332, 1331, 1383
TspGWI ACGGA 2 cut(s) 1220, 1327
TspRI CASTG 2 cut(s) 820, 880
Van91I CCANNNNNTGG 1 cut(s) 950
VneI GTGCAC 1 cut(s) 1021
VpaK11BI GGWCC 1 cut(s) 941
XapI RAATTY 3 cut(s) 125, 169, 490
XceI RCATGY 1 cut(s) 1416
XcmI CCANNNNNNNNNTGG 1 cut(s) 279
XspI CTAG 3 cut(s) 78, 1086, 1404
ZrmI AGTACT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.