MD11G1182600.v1.1

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002703
Physical Location & Seq
Reverse (-)
22485720 .. 22487806
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1182600.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGTGTCTTTATATTTTTGTTCAACTCAAGTTAAAGGAGGCCTTCTTCCCAGAAGCATGGGGATCTCGACCAATGAAGACATTCGATGTTGGGAATAATAACGCCGAGACACAAGATGATTCGCATCAAGCCTTCCTAGAAGCAATGGGAGCGCAAGAAAATACTTTCAATGTGATTGATTATGGTGCTGTTGGGAATGGCCAAGCTGATGATTCAATTGCCTTCCTCAAAGCATGGGGAGCTCTGTGTGTATCAACAACTCGAGGCATCGCTACCCTGGTTGTACCGAAAAGTAAAACATTCTTGTTAAATTCCGTGGCTTTCCAGGGTCCTTGCAGGTCAAGTAACATAAATTTCCAGATACAAGGAAGTATTATTGCTCCAAATAACATTGGTGCATGGAAAAACAAGGACAAATGGATTCAGTTTGCGAATGTGCCCGGTCTTATGATAAACGGCGGAGGTCGAATAGATGGCAACGGTGCCGGTTGGTGGAAATTATGCGGGAACAAGAAATCACACTGCCAGCGACCAACTAGTCTCCACATCCACAAATGTGATGGTTTCCTGCTGAGTCGCCTCACATTCTTGAACAGCCCCAAGAATCATATAAGCATCAACAGCTGCAATGGTGGCCGGGTTTTCGGACTCTTAATTAGTGCTCCGAAAGAAAGCCCTAACACTGATGGAATTGATATTTCTTCATCCACCAAACTTACCATTCACAATTCTCTTATTGCAACTGGCGATGACTGTATTGCCATCAATAGTGGTTCATCTTTTATCAAGATTAATGGTGTTAAATGTGGACCAGGCCATGGTATTAGTATTGGAAGTCTTGGTCAAAATGGAGAACATAGTGCAGTGGAAGAGATTCAAGTAAGCAATTGCAGTTTCAGAGGCACCCAAAATGGAATGAGAATCAAGACATGGGAAGGCGGCTCAGGATATGCGAGGAAGATCACCTTTGAGGGAATTACATTTACAGACATTGAGAACCCAATCATAATAGACCAGCACTATTTTGCTAATCCTACTTCAGTAATGGACATAAACAAGTGCGTACAAGTGAGCGATGTGACATTCCGTAACATTGCTGGATCTTCCGGTGTTTCGCAAGCAATTACATTGGACTGTGGACAGAATTGCTGCACCAACATTATAATGGATAACGTCAATATAAGATCGGGTGTTCCAAACAAGGATGTTTATGCATACTGCAACAATGCCCAGGGCACCTCTTCCTTCTCCACGCCTTACGTCCCCTGCCTTTCCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

427

Amino Acids

45.88

Weight (kDa)

7.85

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 88 - 411 1.1e-90 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1163
AasI GACNNNNNNGTC 1 cut(s) 537
Acc36I ACCTGC 1 cut(s) 327
AccB1I GGYRCC 3 cut(s) 482, 902, 1235
AccB7I CCANNNNNTGG 1 cut(s) 818
AciI CCGC 3 cut(s) 459, 504, 939
AclWI GGATC 2 cut(s) 70, 1108
AcoI YGGCCR 2 cut(s) 199, 634
AcsI RAATTY 2 cut(s) 310, 352
AcuI CTGAAG 1 cut(s) 1023
AfaI GTAC 2 cut(s) 285, 1065
AfiI CCNNNNNNNGG 2 cut(s) 492, 818
AgsI TTSAA 5 cut(s) 23, 169, 216, 592, 878
AhlI ACTAGT 1 cut(s) 536
AjnI CCWGG 4 cut(s) 276, 324, 811, 1230
AleI CACNNNNGTG 1 cut(s) 555
AluBI AGCT 3 cut(s) 206, 242, 624
AluI AGCT 3 cut(s) 206, 242, 624
Alw21I GWGCWC 2 cut(s) 244, 664
Alw26I GTCTC 2 cut(s) 101, 545
AlwI GGATC 2 cut(s) 70, 1108
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 4 cut(s) 39, 199, 634, 814
ApeKI GCWGC 2 cut(s) 624, 1149
ApoI RAATTY 2 cut(s) 310, 352
AseI ATTAAT 1 cut(s) 792
Asp700I GAANNNNTTC 2 cut(s) 80, 873
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 2 cut(s) 329, 809
AsuC2I CCSGG 2 cut(s) 441, 638
AsuHPI GGTGA 1 cut(s) 955
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 2 cut(s) 329, 809
BaeGI GKGCMC 2 cut(s) 441, 1238
BalI TGGCCA 1 cut(s) 201
BanI GGYRCC 3 cut(s) 482, 902, 1235
BanII GRGCYC 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 2 cut(s) 244, 664
BbvI GCAGC 2 cut(s) 611, 1136
BccI CCATC 4 cut(s) 467, 554, 680, 770
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 4 cut(s) 278, 326, 813, 1232
BcnI CCSGG 2 cut(s) 441, 638
BcoDI GTCTC 2 cut(s) 101, 545
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 2 cut(s) 137, 537
BfuAI ACCTGC 1 cut(s) 327
BisI GCNGC 3 cut(s) 625, 940, 1150
BlsI GCNGC 3 cut(s) 626, 941, 1151
Bme1390I CCNGG 6 cut(s) 278, 326, 441, 638, 813, 1232
Bme18I GGWCC 2 cut(s) 329, 809
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 2 cut(s) 329, 809
BmiI GGNNCC 4 cut(s) 330, 484, 904, 1237
BmrFI CCNGG 6 cut(s) 278, 326, 441, 638, 813, 1232
BmsI GCATC 3 cut(s) 133, 276, 624
BpiI GAAGAC 1 cut(s) 83
Bpu10I CCTNAGC 1 cut(s) 943
BpuEI CTTGAG 1 cut(s) 11
BpuMI CCSGG 2 cut(s) 441, 638
BsaBI GATNNNNATC 1 cut(s) 123
BsaJI CCNNGG 6 cut(s) 276, 315, 325, 817, 1230, 1231
BsaWI WCCGGW 1 cut(s) 1106
Bsc4I CCNNNNNNNGG 2 cut(s) 492, 818
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 1 cut(s) 748
Bse3DI GCAATG 3 cut(s) 150, 634, 1092
Bse8I GATNNNNATC 1 cut(s) 123
BseBI CCWGG 4 cut(s) 278, 326, 813, 1232
BseDI CCNNGG 6 cut(s) 276, 315, 325, 817, 1230, 1231
BseGI GGATG 3 cut(s) 546, 704, 1210
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 2 cut(s) 492, 818
BseMI GCAATG 3 cut(s) 150, 634, 1092
BseMII CTCAG 2 cut(s) 563, 957
BseNI ACTGG 1 cut(s) 748
BseSI GKGCMC 2 cut(s) 441, 1238
BseXI GCAGC 2 cut(s) 611, 1136
BsgI GTGCAG 2 cut(s) 882, 1135
BshFI GGCC 4 cut(s) 41, 201, 636, 816
BshNI GGYRCC 3 cut(s) 482, 902, 1235
BsiHKAI GWGCWC 2 cut(s) 244, 664
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 4 cut(s) 441, 486, 637, 1107
BslFI GGGAC 1 cut(s) 1247
BslI CCNNNNNNNGG 2 cut(s) 492, 818
BsmAI GTCTC 2 cut(s) 101, 545
BsmFI GGGAC 1 cut(s) 1247
BsnI GGCC 4 cut(s) 41, 201, 636, 816
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 4 cut(s) 244, 441, 664, 1238
Bsp143I GATC 4 cut(s) 62, 960, 1100, 1184
Bsp19I CCATGG 1 cut(s) 817
BspACI CCGC 3 cut(s) 459, 504, 939
BspANI GGCC 4 cut(s) 41, 201, 636, 816
BspCNI CTCAG 2 cut(s) 564, 956
BspLI GGNNCC 4 cut(s) 330, 484, 904, 1237
BspMI ACCTGC 1 cut(s) 327
BspPI GGATC 2 cut(s) 70, 1108
BspT107I GGYRCC 3 cut(s) 482, 902, 1235
BsrDI GCAATG 3 cut(s) 150, 634, 1092
BsrFI RCCGGY 1 cut(s) 485
BsrI ACTGG 1 cut(s) 748
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 6 cut(s) 276, 315, 325, 817, 1230, 1231
BssMI GATC 4 cut(s) 62, 960, 1100, 1184
BssT1I CCWWGG 1 cut(s) 817
Bst2UI CCWGG 4 cut(s) 278, 326, 813, 1232
Bst4CI ACNGT 3 cut(s) 482, 755, 1136
Bst6I CTCTTC 2 cut(s) 864, 1246
BstC8I GCNNGC 2 cut(s) 527, 1119
BstDEI CTNAG 2 cut(s) 572, 943
BstDSI CCRYGG 2 cut(s) 315, 817
BstF5I GGATG 3 cut(s) 546, 704, 1210
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 4 cut(s) 65, 963, 1103, 1187
BstMAI GTCTC 2 cut(s) 101, 545
BstMBI GATC 4 cut(s) 62, 960, 1100, 1184
BstMWI GCNNNNNNNGC 4 cut(s) 149, 239, 621, 633
BstNI CCWGG 4 cut(s) 278, 326, 813, 1232
BstSCI CCNGG 6 cut(s) 276, 324, 439, 636, 811, 1230
BstSLI GKGCMC 2 cut(s) 441, 1238
BstV1I GCAGC 2 cut(s) 611, 1136
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 2 cut(s) 62, 1100
BstXI CCANNNNNNTGG 1 cut(s) 57
BstYI RGATCY 2 cut(s) 62, 1100
BsuRI GGCC 4 cut(s) 41, 201, 636, 816
BtgI CCRYGG 2 cut(s) 315, 817
BtgZI GCGATG 3 cut(s) 253, 762, 1089
BtsCI GGATG 3 cut(s) 546, 704, 1210
BtsI GCAGTG 2 cut(s) 520, 870
BtsIMutI CAGTG 3 cut(s) 520, 681, 870
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 2 cut(s) 527, 1119
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 485
Cfr13I GGNCC 2 cut(s) 329, 809
Csp6I GTAC 2 cut(s) 284, 1064
CviAII CATG 5 cut(s) 57, 234, 399, 818, 930
CviQI GTAC 2 cut(s) 284, 1064
DdeI CTNAG 2 cut(s) 572, 943
DpnI GATC 4 cut(s) 64, 962, 1102, 1186
DpnII GATC 4 cut(s) 62, 960, 1100, 1184
DrdI GACNNNNNNGTC 1 cut(s) 537
DseDI GACNNNNNNGTC 1 cut(s) 537
EaeI YGGCCR 2 cut(s) 199, 634
Eam1104I CTCTTC 2 cut(s) 864, 1246
EarI CTCTTC 2 cut(s) 864, 1246
EciI GGCGGA 1 cut(s) 474
Ecl136II GAGCTC 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 817
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 329, 809
Eco53kI GAGCTC 1 cut(s) 242
Eco57I CTGAAG 1 cut(s) 1023
Eco88I CYCGRG 1 cut(s) 261
EcoICRI GAGCTC 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 329
EcoRII CCWGG 4 cut(s) 276, 324, 811, 1230
EcoT14I CCWWGG 1 cut(s) 817
EcoT22I ATGCAT 1 cut(s) 1216
EcoT38I GRGCYC 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 817
FaeI CATG 5 cut(s) 60, 237, 402, 821, 933
FalI AAGNNNNNCTT 4 cut(s) 26, 58, 950, 982
FaqI GGGAC 1 cut(s) 1247
FatI CATG 5 cut(s) 56, 233, 398, 817, 929
FauI CCCGC 1 cut(s) 497
Fnu4HI GCNGC 3 cut(s) 625, 940, 1150
FokI GGATG 3 cut(s) 533, 691, 1217
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 3 cut(s) 625, 940, 1150
FspBI CTAG 2 cut(s) 137, 537
GlaI GCGC 1 cut(s) 153
GluI GCNGC 3 cut(s) 625, 940, 1150
HaeIII GGCC 4 cut(s) 41, 201, 636, 816
HapII CCGG 4 cut(s) 441, 486, 637, 1107
HhaI GCGC 1 cut(s) 154
Hin1II CATG 5 cut(s) 60, 237, 402, 821, 933
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HinfI GANTC 8 cut(s) 119, 212, 421, 574, 604, 648, 874, 921
HpaII CCGG 4 cut(s) 441, 486, 637, 1107
HphI GGTGA 1 cut(s) 955
Hpy166II GTNNAC 2 cut(s) 809, 1139
Hpy188I TCNGA 3 cut(s) 647, 666, 899
Hpy188III TCNNGA 6 cut(s) 66, 358, 589, 787, 925, 945
Hpy8I GTNNAC 2 cut(s) 809, 1139
HpyAV CCTTC 5 cut(s) 52, 142, 232, 929, 1255
HpyCH4III ACNGT 3 cut(s) 482, 755, 1136
HpyCH4IV ACGT 2 cut(s) 1173, 1260
HpyCH4V TGCA 9 cut(s) 336, 398, 627, 740, 863, 891, 1152, 1214, 1221
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 239, 621, 633
HpyF3I CTNAG 2 cut(s) 572, 943
HpySE526I ACGT 2 cut(s) 1173, 1260
Hsp92II CATG 5 cut(s) 60, 237, 402, 821, 933
HspAI GCGC 1 cut(s) 152
Kzo9I GATC 4 cut(s) 62, 960, 1100, 1184
LmnI GCTCC 4 cut(s) 149, 239, 385, 667
Lsp1109I GCAGC 2 cut(s) 611, 1136
LweI GCATC 3 cut(s) 133, 276, 624
MaeI CTAG 2 cut(s) 137, 537
MaeII ACGT 2 cut(s) 1173, 1260
MaeIII GTNAC 3 cut(s) 344, 1078, 1088
MalI GATC 4 cut(s) 64, 962, 1102, 1186
MboI GATC 4 cut(s) 62, 960, 1100, 1184
MboII GAAGA 7 cut(s) 37, 88, 693, 881, 970, 1095, 1233
MfeI CAATTG 2 cut(s) 216, 886
MflI RGATCY 2 cut(s) 62, 1100
MhlI GDGCHC 4 cut(s) 244, 441, 664, 1238
MlsI TGGCCA 1 cut(s) 201
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 2 cut(s) 583, 642
MnlI CCTC 9 cut(s) 31, 236, 257, 455, 590, 893, 948, 964, 1249
Mox20I TGGCCA 1 cut(s) 201
Mph1103I ATGCAT 1 cut(s) 1216
MroXI GAANNNNTTC 2 cut(s) 80, 873
MscI TGGCCA 1 cut(s) 201
MseI TTAA 5 cut(s) 32, 308, 653, 792, 801
MslI CAYNNNNRTG 2 cut(s) 555, 1163
Msp20I TGGCCA 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 624
MspI CCGG 4 cut(s) 441, 486, 637, 1107
MspR9I CCNGG 6 cut(s) 278, 326, 441, 638, 813, 1232
MunI CAATTG 2 cut(s) 216, 886
MvaI CCWGG 4 cut(s) 278, 326, 813, 1232
MwoI GCNNNNNNNGC 4 cut(s) 149, 239, 621, 633
NciI CCSGG 2 cut(s) 441, 638
NcoI CCATGG 1 cut(s) 817
NdeII GATC 4 cut(s) 62, 960, 1100, 1184
NlaIII CATG 5 cut(s) 60, 237, 402, 821, 933
NlaIV GGNNCC 4 cut(s) 330, 484, 904, 1237
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 1078
NsiI ATGCAT 1 cut(s) 1216
OliI CACNNNNGTG 1 cut(s) 555
PaeR7I CTCGAG 1 cut(s) 261
PasI CCCWGGG 1 cut(s) 1231
PceI AGGCCT 1 cut(s) 41
PdmI GAANNNNTTC 2 cut(s) 80, 873
PfeI GAWTC 6 cut(s) 119, 212, 421, 604, 874, 921
PflMI CCANNNNNTGG 1 cut(s) 818
PkrI GCNGC 3 cut(s) 626, 941, 1151
PleI GAGTC 2 cut(s) 582, 642
PpsI GAGTC 2 cut(s) 582, 642
PpuMI RGGWCCY 1 cut(s) 329
PshBI ATTAAT 1 cut(s) 792
PsiI TTATAA 1 cut(s) 1163
Psp124BI GAGCTC 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 329
Psp6I CCWGG 4 cut(s) 276, 324, 811, 1230
PspGI CCWGG 4 cut(s) 276, 324, 811, 1230
PspN4I GGNNCC 4 cut(s) 330, 484, 904, 1237
PspPI GGNCC 2 cut(s) 329, 809
PspPPI RGGWCCY 1 cut(s) 329
PspXI VCTCGAGB 1 cut(s) 261
PsuI RGATCY 2 cut(s) 62, 1100
PvuII CAGCTG 1 cut(s) 624
RsaI GTAC 2 cut(s) 285, 1065
RsaNI GTAC 2 cut(s) 284, 1064
RseI CAYNNNNRTG 2 cut(s) 555, 1163
SacI GAGCTC 1 cut(s) 244
SaqAI TTAA 5 cut(s) 32, 308, 653, 792, 801
SatI GCNGC 3 cut(s) 625, 940, 1150
Sau3AI GATC 4 cut(s) 62, 960, 1100, 1184
Sau96I GGNCC 2 cut(s) 329, 809
SchI GAGTC 2 cut(s) 583, 642
ScrFI CCNGG 6 cut(s) 278, 326, 441, 638, 813, 1232
SduI GDGCHC 4 cut(s) 244, 441, 664, 1238
SetI ASST 9 cut(s) 208, 244, 341, 466, 626, 968, 1176, 1241, 1263
SfaNI GCATC 3 cut(s) 133, 276, 624
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 329, 809
SlaI CTCGAG 1 cut(s) 261
SmiMI CAYNNNNRTG 2 cut(s) 555, 1163
SmlI CTYRAG 2 cut(s) 26, 261
SmoI CTYRAG 2 cut(s) 26, 261
SpeI ACTAGT 1 cut(s) 536
SseBI AGGCCT 1 cut(s) 41
SsiI CCGC 3 cut(s) 459, 504, 939
SspMI CTAG 2 cut(s) 137, 537
SstI GAGCTC 1 cut(s) 244
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 6 cut(s) 276, 324, 439, 636, 811, 1230
StyI CCWWGG 1 cut(s) 817
TaaI ACNGT 3 cut(s) 482, 755, 1136
TaiI ACGT 2 cut(s) 1176, 1263
TaqI TCGA 4 cut(s) 67, 84, 262, 466
TauI GCSGC 1 cut(s) 942
TfiI GAWTC 6 cut(s) 119, 212, 421, 604, 874, 921
Tru1I TTAA 5 cut(s) 32, 308, 653, 792, 801
Tru9I TTAA 5 cut(s) 32, 308, 653, 792, 801
TscAI CASTG 3 cut(s) 527, 688, 870
TseFI GTSAC 1 cut(s) 1078
TseI GCWGC 2 cut(s) 624, 1149
Tsp45I GTSAC 1 cut(s) 1078
TspDTI ATGAA 3 cut(s) 89, 693, 765
TspGWI ACGGA 2 cut(s) 304, 1076
TspRI CASTG 3 cut(s) 527, 688, 870
Van91I CCANNNNNTGG 1 cut(s) 818
VpaK11BI GGWCC 2 cut(s) 329, 809
VspI ATTAAT 1 cut(s) 792
XapI RAATTY 2 cut(s) 310, 352
XcmI CCANNNNNNNNNTGG 2 cut(s) 557, 1162
XhoI CTCGAG 1 cut(s) 261
XmnI GAANNNNTTC 2 cut(s) 80, 873
XspI CTAG 2 cut(s) 137, 537
Zsp2I ATGCAT 1 cut(s) 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.