Rroxscaffold_4G00320750

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002703
Physical Location & Seq
Forward (+)
49715567 .. 49717935
2369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320750.1

Sequence Viewer

Length: 1077 bp
ATGGTTGCTTCACCTTCACCAAGTTCATGTGCTAGACTTAATGGTTTTCCCACAACATATACTTTAAGAGACGATGGTGCCATTGGTTATTATGGTGATCAAGTTGCTGCTGCGACATCAAATCCATTCCATAATAAGGAGTACGGTGATGTTTTTGCAACCCCAAATACATTCACACAGTATTATCGTCCTCTTGCAAATCGCCAAACCGATGATGATTTACAAGCATCCCCAGTTGCATGGAGATCACTTGCAAAAAGCGAATTCAATGTGATGAATTATGGCGCCGTTGGGAATGGCCAAGTTGATGATACACAAGCTCTTCATATTAGCGAGTGTGATGGTCTCCGCTTGAGTAATATTACACATATTAATAGTGCGAGAAACCATATAAGCATCACCGGCTGCAATGGCGTTCAAATTTCTGATATCCTTATTAGGGCTCCTGATGAAAGTCCAAACACTGATGGAATTGACATCTCTGCATCAACCAATATTAACATTCACAATTCTTTTATAGGCACTGGTGACGACTGTATTGCAATCATTTCTGGTTCATCTCATATCAACATTACTGATGTTATGTGTGGTCCAGGCCATGGTATAAGTGTTGGAAGTCTTGGTAAGCAAGGTTCTTATAGCACAGTGGAAGATGTGCAAGTGAGAAACTGCACCTTCAACGGAACACAAAATGGAGCAAGAATCAAGACATGGCAAGGCGGGTCAGGGTATGCTAGGAACATCAGTTTTGAGGATATCATAATTCAAGCAGCCGAGAACCCTATCATTATAGACCAGAAGTATACTGATAATCTCTCTACTGGAACTGGAACTGGAACCCTGCTTGACCAAGAAAATGCTGTACAAGTGAGCGATGTGACCTTCCGTAACATTCGGGGATCTGTTGCTGGGGATACAGCCATTACTTTGGACTGTGACGATCATATTGGTTGCAAAAACATTGTAATGGATGACATAGACTTAACTTCATCTGTTCCCGGCAAGAAGGTTTCTACCCGCTGCAAGAACGTTAAAGGATTATCTACTTTATTATCTCCCAGTGTGTCATGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

358

Amino Acids

38.1

Weight (kDa)

4.84

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 104 - 345 1e-72 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 77, 284
AccB7I CCANNNNNTGG 1 cut(s) 599
AccI GTMKAC 1 cut(s) 803
AciI CCGC 3 cut(s) 349, 720, 1018
AclI AACGTT 1 cut(s) 1029
AclWI GGATC 1 cut(s) 907
AcoI YGGCCR 1 cut(s) 298
AcsI RAATTY 2 cut(s) 263, 420
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 2 cut(s) 143, 864
AfiI CCNNNNNNNGG 3 cut(s) 136, 439, 599
AgsI TTSAA 4 cut(s) 268, 419, 679, 767
AjnI CCWGG 1 cut(s) 592
AluBI AGCT 1 cut(s) 320
AluI AGCT 1 cut(s) 320
Alw26I GTCTC 2 cut(s) 63, 350
AlwI GGATC 1 cut(s) 907
AoxI GGCC 2 cut(s) 298, 595
ApeKI GCWGC 5 cut(s) 107, 110, 405, 770, 1020
ApoI RAATTY 2 cut(s) 263, 420
AseI ATTAAT 1 cut(s) 372
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 1 cut(s) 590
AsuC2I CCSGG 1 cut(s) 999
AsuHPI GGTGA 6 cut(s) 3, 9, 107, 158, 391, 539
AvaII GGWCC 1 cut(s) 590
BalI TGGCCA 1 cut(s) 300
BanI GGYRCC 2 cut(s) 77, 284
BanII GRGCYC 1 cut(s) 445
BbvI GCAGC 5 cut(s) 94, 97, 392, 782, 1007
BccI CCATC 3 cut(s) 68, 335, 461
BceAI ACGGC 1 cut(s) 272
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 1 cut(s) 594
BciVI GTATCC 1 cut(s) 907
BclI TGATCA 1 cut(s) 97
BcnI CCSGG 1 cut(s) 999
BcoDI GTCTC 2 cut(s) 63, 350
BfaI CTAG 2 cut(s) 33, 735
BfoI RGCGCY 1 cut(s) 288
BfuI GTATCC 1 cut(s) 907
BisI GCNGC 5 cut(s) 108, 111, 406, 771, 1021
BlsI GCNGC 5 cut(s) 109, 112, 407, 772, 1022
Bme1390I CCNGG 2 cut(s) 594, 999
Bme18I GGWCC 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 4 cut(s) 79, 286, 444, 838
BmrFI CCNGG 2 cut(s) 594, 999
BmrI ACTGGG 2 cut(s) 227, 1053
BmsI GCATC 3 cut(s) 236, 405, 494
BmuI ACTGGG 2 cut(s) 227, 1053
BpuEI CTTGAG 1 cut(s) 373
BpuMI CCSGG 1 cut(s) 999
BsaHI GRCGYC 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 1 cut(s) 598
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 439, 599
Bse118I RCCGGY 1 cut(s) 401
Bse1I ACTGG 6 cut(s) 233, 529, 826, 832, 838, 1059
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 594
BseDI CCNNGG 1 cut(s) 598
BseGI GGATG 2 cut(s) 227, 976
BseLI CCNNNNNNNGG 3 cut(s) 136, 439, 599
BseMI GCAATG 1 cut(s) 415
BseNI ACTGG 6 cut(s) 233, 529, 826, 832, 838, 1059
BseXI GCAGC 5 cut(s) 94, 97, 392, 782, 1007
BseYI CCCAGC 1 cut(s) 908
BsgI GTGCAG 1 cut(s) 655
BshFI GGCC 2 cut(s) 300, 597
BshNI GGYRCC 2 cut(s) 77, 284
BsiSI CCGG 2 cut(s) 402, 999
BslI CCNNNNNNNGG 3 cut(s) 136, 439, 599
BsmAI GTCTC 2 cut(s) 63, 350
BsmBI CGTCTC 1 cut(s) 63
BsnI GGCC 2 cut(s) 300, 597
Bso31I GGTCTC 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 445
Bsp1407I TGTACA 1 cut(s) 862
Bsp143I GATC 4 cut(s) 97, 245, 899, 940
Bsp19I CCATGG 1 cut(s) 598
BspACI CCGC 3 cut(s) 349, 720, 1018
BspANI GGCC 2 cut(s) 300, 597
BspLI GGNNCC 4 cut(s) 79, 286, 444, 838
BspPI GGATC 1 cut(s) 907
BspQI GCTCTTC 1 cut(s) 327
BspT107I GGYRCC 2 cut(s) 77, 284
BspTNI GGTCTC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 415
BsrFI RCCGGY 1 cut(s) 401
BsrGI TGTACA 1 cut(s) 862
BsrI ACTGG 6 cut(s) 233, 529, 826, 832, 838, 1059
BssAI RCCGGY 1 cut(s) 401
BssECI CCNNGG 1 cut(s) 598
BssMI GATC 4 cut(s) 97, 245, 899, 940
BssNAI GTATAC 1 cut(s) 804
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 598
Bst1107I GTATAC 1 cut(s) 804
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 5 cut(s) 146, 180, 536, 646, 935
Bst6I CTCTTC 1 cut(s) 327
BstACI GRCGYC 1 cut(s) 285
BstAUI TGTACA 1 cut(s) 862
BstDSI CCRYGG 1 cut(s) 598
BstF5I GGATG 2 cut(s) 227, 976
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 4 cut(s) 100, 248, 902, 943
BstMAI GTCTC 2 cut(s) 63, 350
BstMBI GATC 4 cut(s) 97, 245, 899, 940
BstMWI GCNNNNNNNGC 2 cut(s) 402, 411
BstNI CCWGG 1 cut(s) 594
BstSCI CCNGG 2 cut(s) 592, 997
BstV1I GCAGC 5 cut(s) 94, 97, 392, 782, 1007
BstX2I RGATCY 1 cut(s) 899
BstXI CCANNNNNNTGG 2 cut(s) 240, 928
BstYI RGATCY 1 cut(s) 899
BstZ17I GTATAC 1 cut(s) 804
BsuI GTATCC 1 cut(s) 907
BsuRI GGCC 2 cut(s) 300, 597
BtgI CCRYGG 1 cut(s) 598
BtgZI GCGATG 1 cut(s) 888
BtsCI GGATG 2 cut(s) 227, 976
BtsIMutI CAGTG 4 cut(s) 462, 522, 651, 1066
CfoI GCGC 1 cut(s) 287
Cfr10I RCCGGY 1 cut(s) 401
Cfr13I GGNCC 1 cut(s) 590
Csp6I GTAC 2 cut(s) 142, 863
CviAII CATG 5 cut(s) 27, 240, 599, 711, 1068
CviJI RGCY 7 cut(s) 300, 320, 405, 443, 597, 773, 920
CviKI_1 RGCY 7 cut(s) 300, 320, 405, 443, 597, 773, 920
CviQI GTAC 2 cut(s) 142, 863
DinI GGCGCC 1 cut(s) 286
DpnI GATC 4 cut(s) 99, 247, 901, 942
DpnII GATC 4 cut(s) 97, 245, 899, 940
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 445
Eco31I GGTCTC 1 cut(s) 350
Eco32I GATATC 2 cut(s) 430, 757
Eco47I GGWCC 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 592
EcoRV GATATC 2 cut(s) 430, 757
EcoT14I CCWWGG 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 445
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 598
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 5 cut(s) 30, 243, 602, 714, 1071
FatI CATG 5 cut(s) 26, 239, 598, 710, 1067
FauI CCCGC 2 cut(s) 713, 1025
FbaI TGATCA 1 cut(s) 97
FblI GTMKAC 1 cut(s) 803
Fnu4HI GCNGC 5 cut(s) 108, 111, 406, 771, 1021
FokI GGATG 2 cut(s) 214, 983
FriOI GRGCYC 1 cut(s) 445
Fsp4HI GCNGC 5 cut(s) 108, 111, 406, 771, 1021
FspBI CTAG 2 cut(s) 33, 735
GlaI GCGC 1 cut(s) 286
GluI GCNGC 5 cut(s) 108, 111, 406, 771, 1021
GsaI CCCAGC 1 cut(s) 912
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 2 cut(s) 300, 597
HapII CCGG 2 cut(s) 402, 999
HhaI GCGC 1 cut(s) 287
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 5 cut(s) 30, 243, 602, 714, 1071
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 1 cut(s) 702
HpaII CCGG 2 cut(s) 402, 999
HphI GGTGA 6 cut(s) 3, 9, 107, 158, 391, 539
Hpy166II GTNNAC 1 cut(s) 804
Hpy188I TCNGA 1 cut(s) 427
Hpy188III TCNNGA 2 cut(s) 446, 706
Hpy8I GTNNAC 1 cut(s) 804
HpyAV CCTTC 4 cut(s) 24, 685, 892, 1000
HpyCH4III ACNGT 5 cut(s) 146, 180, 536, 646, 935
HpyCH4IV ACGT 1 cut(s) 1029
HpyF10VI GCNNNNNNNGC 2 cut(s) 402, 411
HpySE526I ACGT 1 cut(s) 1029
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 5 cut(s) 30, 243, 602, 714, 1071
HspAI GCGC 1 cut(s) 285
KasI GGCGCC 1 cut(s) 284
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 4 cut(s) 97, 245, 899, 940
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 2 cut(s) 448, 695
Lsp1109I GCAGC 5 cut(s) 94, 97, 392, 782, 1007
LweI GCATC 3 cut(s) 236, 405, 494
MaeI CTAG 2 cut(s) 33, 735
MaeII ACGT 1 cut(s) 1029
MaeIII GTNAC 4 cut(s) 527, 877, 887, 935
MalI GATC 4 cut(s) 99, 247, 901, 942
MboI GATC 4 cut(s) 97, 245, 899, 940
MboII GAAGA 2 cut(s) 314, 662
MflI RGATCY 1 cut(s) 899
MhlI GDGCHC 1 cut(s) 445
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 6 cut(s) 263, 277, 420, 471, 508, 762
MluNI TGGCCA 1 cut(s) 300
Mly113I GGCGCC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 592
MnlI CCTC 2 cut(s) 201, 745
Mox20I TGGCCA 1 cut(s) 300
MscI TGGCCA 1 cut(s) 300
MseI TTAA 7 cut(s) 39, 65, 372, 498, 983, 1032, 1075
MslI CAYNNNNRTG 1 cut(s) 965
Msp20I TGGCCA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 1020
MspI CCGG 2 cut(s) 402, 999
MspR9I CCNGG 2 cut(s) 594, 999
MvaI CCWGG 1 cut(s) 594
MwoI GCNNNNNNNGC 2 cut(s) 402, 411
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 1 cut(s) 999
NcoI CCATGG 1 cut(s) 598
NdeII GATC 4 cut(s) 97, 245, 899, 940
NlaIII CATG 5 cut(s) 30, 243, 602, 714, 1071
NlaIV GGNNCC 4 cut(s) 79, 286, 444, 838
NmeAIII GCCGAG 1 cut(s) 799
NmuCI GTSAC 3 cut(s) 527, 877, 935
PciSI GCTCTTC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 702
PflMI CCANNNNNTGG 1 cut(s) 599
PkrI GCNGC 5 cut(s) 109, 112, 407, 772, 1022
PluTI GGCGCC 1 cut(s) 288
PshBI ATTAAT 1 cut(s) 372
Psp1406I AACGTT 1 cut(s) 1029
Psp6I CCWGG 1 cut(s) 592
PspFI CCCAGC 1 cut(s) 908
PspGI CCWGG 1 cut(s) 592
PspN4I GGNNCC 4 cut(s) 79, 286, 444, 838
PspPI GGNCC 1 cut(s) 590
PsrI GAACNNNNNNTAC 2 cut(s) 616, 648
PsuI RGATCY 1 cut(s) 899
RsaI GTAC 2 cut(s) 143, 864
RsaNI GTAC 2 cut(s) 142, 863
RseI CAYNNNNRTG 1 cut(s) 965
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 7 cut(s) 39, 65, 372, 498, 983, 1032, 1075
SatI GCNGC 5 cut(s) 108, 111, 406, 771, 1021
Sau3AI GATC 4 cut(s) 97, 245, 899, 940
Sau96I GGNCC 1 cut(s) 590
ScrFI CCNGG 2 cut(s) 594, 999
SduI GDGCHC 1 cut(s) 445
SetI ASST 7 cut(s) 16, 322, 634, 677, 884, 1011, 1032
SfaNI GCATC 3 cut(s) 236, 405, 494
SfoI GGCGCC 1 cut(s) 286
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 965
SmlI CTYRAG 1 cut(s) 352
SmoI CTYRAG 1 cut(s) 352
Sse9I AATT 6 cut(s) 263, 277, 420, 471, 508, 762
SsiI CCGC 3 cut(s) 349, 720, 1018
SspDI GGCGCC 1 cut(s) 284
SspI AATATT 2 cut(s) 361, 496
SspMI CTAG 2 cut(s) 33, 735
StyD4I CCNGG 2 cut(s) 592, 997
StyI CCWWGG 1 cut(s) 598
TaaI ACNGT 5 cut(s) 146, 180, 536, 646, 935
TaiI ACGT 1 cut(s) 1032
TasI AATT 6 cut(s) 263, 277, 420, 471, 508, 762
TatI WGTACW 1 cut(s) 862
TfiI GAWTC 1 cut(s) 702
Tru1I TTAA 7 cut(s) 39, 65, 372, 498, 983, 1032, 1075
Tru9I TTAA 7 cut(s) 39, 65, 372, 498, 983, 1032, 1075
TscAI CASTG 4 cut(s) 469, 529, 651, 1066
TseFI GTSAC 3 cut(s) 527, 877, 935
TseI GCWGC 5 cut(s) 107, 110, 405, 770, 1020
Tsp45I GTSAC 3 cut(s) 527, 877, 935
TspDTI ATGAA 6 cut(s) 15, 290, 314, 465, 546, 978
TspGWI ACGGA 2 cut(s) 696, 875
TspRI CASTG 4 cut(s) 469, 529, 651, 1066
Van91I CCANNNNNTGG 1 cut(s) 599
VpaK11BI GGWCC 1 cut(s) 590
VspI ATTAAT 1 cut(s) 372
XapI RAATTY 2 cut(s) 263, 420
XmiI GTMKAC 1 cut(s) 803
XspI CTAG 2 cut(s) 33, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.