Rw1G007970

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002703
Physical Location & Seq
Reverse (-)
17072738 .. 17075105
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G007970.1

Sequence Viewer

Length: 1338 bp
ATGGAGGGTCGAATACTTGCTACGATGTTTTTGTTCATGATCATTATGGTTGCTTCACCTTCACCAAGTTCATGTGCCAGACTTAATGGTTTTCCCACAACATATACGTTAAGAGACGATGATACCATTGGTTATTATGGTGATCAATATGGTGATGTTTTTGCAACCCCAAATACATTCAAAGAGTATTATCGTCCTCTTGCAAATCGCCAAACTGATGATGATTTACAAGCATTCCCAGATGCATGGAGATCACTTGCAAAAAGCGAATTCAATGTGATGAATTATGGCGCTGTTGGGAATGGCCAAGTTGATGATACACAAGCCTTTCTAAAAGCATGGGGAGATGTTTGTGGATCCACTCAAGGCATCCCTACACTTATTATACCAAAGGGCAACACTTTCTTGCTCAATTCTGTGGAGTTTAAAGGACCTTGCAAGGCTGCAAGTGTAAATTTCCAGCTAAGTGGGAACATTGTTGCACCAAATAATATAAATGCTTGGAAAGACAAGGACAAATGGATTCAGTTTTATGATGTTGAAGGGCTGATAATCAATGGAGGAGGTCAAATTGATGGTCAAGGTGAGGTTTGGTGGAAAGTTTGCGACAATTCAAATTGCCAACGACCAGCAGCTGTAAGTCAGATCAACAATTGTGATGGTCTCCGCTTGAGTGATATTACACATATTAATAGTCCGAGAAACCATATATTCATCATGGGCAGCAATGGCGTTCAAATTTCAGATATGCTTATTAGGGCTCCTGATGAAAGTCCAAACACTGATGGAATTAAAATCTCTAGATCAACCAATGTTAACATTCAGAATTCTTTTATAGGCACTGGTGACGACTGTATTGCAATCATTTCTGGTTCATCTCGTATCAACATTACTAATGTTATGTGTGGTCCAGGCCATGGAATAAGTGTTGGAAGTCTTGGTAAGCAAGGTGCTTATAACACAGTGGAAGATGTGCAAGTGAGAAACTGCACCCTGAAGGGAACACAAAATGGAGCAAGAATCAAGACATGGCAAGGCGGGTCAGGGTATGCTAGGAACATCAGTTTTGAGGATATCATAATTCAAGCAGTCAAGAACCCCATCATTATAAACCAGAATGCTGTAAAAGTGAGCGATGTGACATTCCGTAACATTCGGGGATCTGTTGCTGGGGAAACAGCCATTACTTTGGACTGTGACGATCAAATTGGTTGCAAAAACATTGTGATGGATAACATAGACTTAACTTCATCTGTTCCTGGCAAGAAGGTTTCTGCCCAGTGCAAGAACGTTCAAGGATTTTCTACTTCATTATCTCCTAGTGTGCCATGCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

445

Amino Acids

48.17

Weight (kDa)

5.36

Isoelectric Point (pI)

30.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 122 - 432 3.6e-85 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 957, 1109
AccB7I CCANNNNNTGG 1 cut(s) 917
AciI CCGC 2 cut(s) 667, 1038
AclI AACGTT 1 cut(s) 1290
AclWI GGATC 3 cut(s) 351, 364, 1168
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 4 cut(s) 269, 454, 738, 826
AcuI CTGAAG 1 cut(s) 1016
AfiI CCNNNNNNNGG 1 cut(s) 917
AgsI TTSAA 7 cut(s) 181, 274, 542, 615, 737, 1085, 1295
AjnI CCWGG 2 cut(s) 910, 1258
AluBI AGCT 2 cut(s) 463, 635
AluI AGCT 2 cut(s) 463, 635
Alw26I GTCTC 2 cut(s) 108, 668
AlwI GGATC 3 cut(s) 351, 364, 1168
AlwNI CAGNNNCTG 1 cut(s) 635
AoxI GGCC 2 cut(s) 304, 913
ApeKI GCWGC 3 cut(s) 443, 632, 723
ApoI RAATTY 4 cut(s) 269, 454, 738, 826
ArsI GACNNNNNNTTYG 2 cut(s) 562, 594
AseI ATTAAT 1 cut(s) 690
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 431, 908
AsuHPI GGTGA 6 cut(s) 48, 54, 152, 164, 596, 857
AvaII GGWCC 2 cut(s) 431, 908
BalI TGGCCA 1 cut(s) 306
BamHI GGATCC 1 cut(s) 356
BanII GRGCYC 1 cut(s) 763
BbvI GCAGC 3 cut(s) 430, 644, 735
BccI CCATC 5 cut(s) 569, 653, 779, 1109, 1222
BcgI CGANNNNNNTGC 2 cut(s) 839, 873
BciT130I CCWGG 2 cut(s) 912, 1260
BclI TGATCA 2 cut(s) 39, 142
BcoDI GTCTC 2 cut(s) 108, 668
BfaI CTAG 3 cut(s) 801, 1053, 1320
BfoI RGCGCY 1 cut(s) 294
BisI GCNGC 3 cut(s) 444, 633, 724
BlsI GCNGC 3 cut(s) 445, 634, 725
Bme1390I CCNGG 2 cut(s) 912, 1260
Bme18I GGWCC 2 cut(s) 431, 908
BmgT120I GGNCC 2 cut(s) 431, 908
BmiI GGNNCC 2 cut(s) 358, 762
BmrFI CCNGG 2 cut(s) 912, 1260
BmrI ACTGGG 1 cut(s) 1273
BmsI GCATC 2 cut(s) 232, 378
BmuI ACTGGG 1 cut(s) 1273
BpuEI CTTGAG 2 cut(s) 348, 691
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 1 cut(s) 916
Bsc4I CCNNNNNNNGG 1 cut(s) 917
Bse1I ACTGG 2 cut(s) 847, 1279
Bse3DI GCAATG 1 cut(s) 733
BseBI CCWGG 2 cut(s) 912, 1260
BseDI CCNNGG 1 cut(s) 916
BseGI GGATG 1 cut(s) 369
BseLI CCNNNNNNNGG 1 cut(s) 917
BseMI GCAATG 1 cut(s) 733
BseNI ACTGG 2 cut(s) 847, 1279
BseRI GAGGAG 1 cut(s) 576
BseXI GCAGC 3 cut(s) 430, 644, 735
BseYI CCCAGC 1 cut(s) 1169
BsgI GTGCAG 1 cut(s) 973
BshFI GGCC 2 cut(s) 306, 915
BslI CCNNNNNNNGG 1 cut(s) 917
BsmAI GTCTC 2 cut(s) 108, 668
BsmBI CGTCTC 1 cut(s) 108
BsmI GAATGC 2 cut(s) 233, 1123
BsnI GGCC 2 cut(s) 306, 915
Bso31I GGTCTC 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 763
Bsp143I GATC 8 cut(s) 39, 142, 251, 356, 645, 803, 1160, 1201
Bsp19I CCATGG 1 cut(s) 916
BspACI CCGC 2 cut(s) 667, 1038
BspANI GGCC 2 cut(s) 306, 915
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 358, 762
BspPI GGATC 3 cut(s) 351, 364, 1168
BspTNI GGTCTC 1 cut(s) 668
BsrDI GCAATG 1 cut(s) 733
BsrI ACTGG 2 cut(s) 847, 1279
BssECI CCNNGG 1 cut(s) 916
BssMI GATC 8 cut(s) 39, 142, 251, 356, 645, 803, 1160, 1201
BssT1I CCWWGG 1 cut(s) 916
Bst2UI CCWGG 2 cut(s) 912, 1260
Bst4CI ACNGT 3 cut(s) 854, 964, 1196
BstDEI CTNAG 1 cut(s) 464
BstDSI CCRYGG 1 cut(s) 916
BstF5I GGATG 1 cut(s) 369
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 8 cut(s) 42, 145, 254, 359, 648, 806, 1163, 1204
BstMAI GTCTC 2 cut(s) 108, 668
BstMBI GATC 8 cut(s) 39, 142, 251, 356, 645, 803, 1160, 1201
BstMWI GCNNNNNNNGC 1 cut(s) 729
BstNI CCWGG 2 cut(s) 912, 1260
BstSCI CCNGG 2 cut(s) 910, 1258
BstV1I GCAGC 3 cut(s) 430, 644, 735
BstX2I RGATCY 2 cut(s) 356, 1160
BstXI CCANNNNNNTGG 3 cut(s) 246, 467, 1189
BstYI RGATCY 2 cut(s) 356, 1160
BsuRI GGCC 2 cut(s) 306, 915
BtgI CCRYGG 1 cut(s) 916
BtgZI GCGATG 1 cut(s) 1149
BtsCI GGATG 1 cut(s) 369
BtsIMutI CAGTG 4 cut(s) 780, 840, 969, 1286
CaiI CAGNNNCTG 1 cut(s) 635
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 431, 908
CviAII CATG 8 cut(s) 37, 72, 246, 339, 718, 917, 1029, 1329
CviJI RGCY 9 cut(s) 306, 326, 443, 463, 547, 635, 761, 915, 1181
CviKI_1 RGCY 9 cut(s) 306, 326, 443, 463, 547, 635, 761, 915, 1181
DdeI CTNAG 1 cut(s) 464
DpnI GATC 8 cut(s) 41, 144, 253, 358, 647, 805, 1162, 1203
DpnII GATC 8 cut(s) 39, 142, 251, 356, 645, 803, 1160, 1201
DraI TTTAAA 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 916
Eco24I GRGCYC 1 cut(s) 763
Eco31I GGTCTC 1 cut(s) 668
Eco32I GATATC 1 cut(s) 1075
Eco47I GGWCC 2 cut(s) 431, 908
Eco57I CTGAAG 1 cut(s) 1016
EcoO109I RGGNCCY 1 cut(s) 431
EcoRI GAATTC 2 cut(s) 269, 826
EcoRII CCWGG 2 cut(s) 910, 1258
EcoRV GATATC 1 cut(s) 1075
EcoT14I CCWWGG 1 cut(s) 916
EcoT22I ATGCAT 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 763
ErhI CCWWGG 1 cut(s) 916
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 8 cut(s) 40, 75, 249, 342, 721, 920, 1032, 1332
FatI CATG 8 cut(s) 36, 71, 245, 338, 717, 916, 1028, 1328
FauI CCCGC 1 cut(s) 1031
FbaI TGATCA 2 cut(s) 39, 142
Fnu4HI GCNGC 3 cut(s) 444, 633, 724
FokI GGATG 1 cut(s) 356
FriOI GRGCYC 1 cut(s) 763
Fsp4HI GCNGC 3 cut(s) 444, 633, 724
FspBI CTAG 3 cut(s) 801, 1053, 1320
GlaI GCGC 1 cut(s) 292
GluI GCNGC 3 cut(s) 444, 633, 724
GsaI CCCAGC 1 cut(s) 1173
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 2 cut(s) 306, 915
HhaI GCGC 1 cut(s) 293
Hin1II CATG 8 cut(s) 40, 75, 249, 342, 721, 920, 1032, 1332
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HinfI GANTC 2 cut(s) 523, 1020
HpaI GTTAAC 1 cut(s) 817
HphI GGTGA 6 cut(s) 48, 54, 152, 164, 596, 857
Hpy166II GTNNAC 1 cut(s) 817
Hpy188I TCNGA 4 cut(s) 645, 699, 745, 825
Hpy188III TCNNGA 5 cut(s) 37, 764, 801, 1024, 1093
Hpy8I GTNNAC 1 cut(s) 817
HpyAV CCTTC 4 cut(s) 69, 536, 991, 1261
HpyCH4III ACNGT 3 cut(s) 854, 964, 1196
HpyCH4IV ACGT 2 cut(s) 107, 1290
HpyF10VI GCNNNNNNNGC 1 cut(s) 729
HpyF3I CTNAG 1 cut(s) 464
HpySE526I ACGT 2 cut(s) 107, 1290
Hsp92II CATG 8 cut(s) 40, 75, 249, 342, 721, 920, 1032, 1332
HspAI GCGC 1 cut(s) 291
Ksp22I TGATCA 2 cut(s) 39, 142
KspAI GTTAAC 1 cut(s) 817
Kzo9I GATC 8 cut(s) 39, 142, 251, 356, 645, 803, 1160, 1201
LmnI GCTCC 2 cut(s) 766, 1013
Lsp1109I GCAGC 3 cut(s) 430, 644, 735
LweI GCATC 2 cut(s) 232, 378
MaeI CTAG 3 cut(s) 801, 1053, 1320
MaeII ACGT 2 cut(s) 107, 1290
MaeIII GTNAC 4 cut(s) 845, 1138, 1148, 1196
MalI GATC 8 cut(s) 41, 144, 253, 358, 647, 805, 1162, 1203
MboI GATC 8 cut(s) 39, 142, 251, 356, 645, 803, 1160, 1201
MboII GAAGA 1 cut(s) 980
MfeI CAATTG 1 cut(s) 652
MflI RGATCY 2 cut(s) 356, 1160
MhlI GDGCHC 1 cut(s) 763
MlsI TGGCCA 1 cut(s) 306
MluNI TGGCCA 1 cut(s) 306
MmeI TCCRAC 1 cut(s) 910
MnlI CCTC 5 cut(s) 207, 554, 557, 580, 1063
Mox20I TGGCCA 1 cut(s) 306
Mph1103I ATGCAT 1 cut(s) 247
MscI TGGCCA 1 cut(s) 306
MseI TTAA 8 cut(s) 84, 110, 426, 690, 792, 816, 1244, 1336
MslI CAYNNNNRTG 1 cut(s) 1226
Msp20I TGGCCA 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 635
MspR9I CCNGG 2 cut(s) 912, 1260
MunI CAATTG 1 cut(s) 652
Mva1269I GAATGC 2 cut(s) 233, 1123
MvaI CCWGG 2 cut(s) 912, 1260
MwoI GCNNNNNNNGC 1 cut(s) 729
NcoI CCATGG 1 cut(s) 916
NdeII GATC 8 cut(s) 39, 142, 251, 356, 645, 803, 1160, 1201
NlaIII CATG 8 cut(s) 40, 75, 249, 342, 721, 920, 1032, 1332
NlaIV GGNNCC 2 cut(s) 358, 762
NmuCI GTSAC 3 cut(s) 845, 1138, 1196
NsiI ATGCAT 1 cut(s) 247
PagI TCATGA 1 cut(s) 36
PctI GAATGC 2 cut(s) 233, 1123
PfeI GAWTC 2 cut(s) 523, 1020
PflMI CCANNNNNTGG 1 cut(s) 917
PkrI GCNGC 3 cut(s) 445, 634, 725
PpuMI RGGWCCY 1 cut(s) 431
PshBI ATTAAT 1 cut(s) 690
PsiI TTATAA 2 cut(s) 957, 1109
Psp1406I AACGTT 1 cut(s) 1290
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 2 cut(s) 910, 1258
PspFI CCCAGC 1 cut(s) 1169
PspGI CCWGG 2 cut(s) 910, 1258
PspN4I GGNNCC 2 cut(s) 358, 762
PspPI GGNCC 2 cut(s) 431, 908
PspPPI RGGWCCY 1 cut(s) 431
PstNI CAGNNNCTG 1 cut(s) 635
PsuI RGATCY 2 cut(s) 356, 1160
PvuII CAGCTG 1 cut(s) 635
RseI CAYNNNNRTG 1 cut(s) 1226
SaqAI TTAA 8 cut(s) 84, 110, 426, 690, 792, 816, 1244, 1336
SatI GCNGC 3 cut(s) 444, 633, 724
Sau3AI GATC 8 cut(s) 39, 142, 251, 356, 645, 803, 1160, 1201
Sau96I GGNCC 2 cut(s) 431, 908
ScrFI CCNGG 2 cut(s) 912, 1260
SduI GDGCHC 1 cut(s) 763
SfaNI GCATC 2 cut(s) 232, 378
SinI GGWCC 2 cut(s) 431, 908
SmiMI CAYNNNNRTG 1 cut(s) 1226
SmlI CTYRAG 2 cut(s) 363, 670
SmoI CTYRAG 2 cut(s) 363, 670
SsiI CCGC 2 cut(s) 667, 1038
SspMI CTAG 3 cut(s) 801, 1053, 1320
StyD4I CCNGG 2 cut(s) 910, 1258
StyI CCWWGG 1 cut(s) 916
TaaI ACNGT 3 cut(s) 854, 964, 1196
TaiI ACGT 2 cut(s) 110, 1293
TaqI TCGA 1 cut(s) 10
TfiI GAWTC 2 cut(s) 523, 1020
Tru1I TTAA 8 cut(s) 84, 110, 426, 690, 792, 816, 1244, 1336
Tru9I TTAA 8 cut(s) 84, 110, 426, 690, 792, 816, 1244, 1336
TscAI CASTG 4 cut(s) 787, 847, 969, 1286
TseFI GTSAC 3 cut(s) 845, 1138, 1196
TseI GCWGC 3 cut(s) 443, 632, 723
Tsp45I GTSAC 3 cut(s) 845, 1138, 1196
TspDTI ATGAA 8 cut(s) 25, 60, 296, 703, 783, 864, 1239, 1299
TspGWI ACGGA 1 cut(s) 1136
TspRI CASTG 4 cut(s) 787, 847, 969, 1286
Van91I CCANNNNNTGG 1 cut(s) 917
VpaK11BI GGWCC 2 cut(s) 431, 908
VspI ATTAAT 1 cut(s) 690
XapI RAATTY 4 cut(s) 269, 454, 738, 826
XbaI TCTAGA 1 cut(s) 800
XspI CTAG 3 cut(s) 801, 1053, 1320
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.