Rh4CG311000

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002703
Physical Location & Seq
Forward (+)
57250729 .. 57252067
1339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG311000.1

Sequence Viewer

Length: 984 bp
ATGGACTTCACAACAATATTGCTGAACGTGGATCTTAAATTACAGCTCCAAGGGAGACTAGTTGCACCAGATTATAACAGTAAAGCATGGGAATCAGACAAGGAAAAATGGGTTCAGTTCTCAGATGTAGATGGTCTCATAATCATTGGAGGAGGTCTAGGTGTAATTGATGGTAAGGGTTCCAATTGGTGGAAAGCTTGCGACAAGTCAGACTGCGATCGACCAGCGGCTCTTGAAATTCGTAAATGTGATAATCTCCGACTGAGTGGTGTGACACATATTAATAGCGCAAAAGCCCATATAAAATTCTATGGGTGCAACGGAGTTCAAGTTTCTGGTCTGCTTATTAGCGCTCCTGATGAAAGTCCGAACACAGATGGGATTCAGATCATTTCATCAACTAATGTTAACATTGACAATTCTGCTATAGAAACTGGTGATGACTGCATTGCCATCAGTAGTGGTTCTTCTCAGATCCAAGGTAGTGGTGTTCGATGTGGACCTGGCCATGGTATAAGCATTGGAAGTCTTGGGAAAAATGGAGCTTACGCCACAGTGGAAGACGTGAACGTGAGAAATTGCACCTTGAGAGGAACGCAAAATGGACTACGTATCAAGACATGGCCAGGTGGAAGAGGTTATGCTAGGAATATCACATTTGAGGGAATCCGACTTATAGGGTCCAAGAACCCTATCATAATAAACCAGCACTATGTCGATAAAGGCCTGTCCGGGCCAAAAGAAGCTGACCGGAAAAGCTCAGTACAAGTGGGTAATGTGACGTACCATGGAGTGTTTGGATCTTCTTCCAATGAGGTAGCTATCAATTTGAACTGCGCTGGGGGGAGGCGCGGTTGCGACAACATTACAATGGATAACATTCACATTACTTCATCCCTTCCCGGGCAGAAGACTTCTGCCTACTGCAACAATGCCCATGGTACAGCTACACTTGCTATCCCTAATGTCCCATGCCTCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

327

Amino Acids

34.67

Weight (kDa)

7.53

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 10 - 315 6.1e-81 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 75
AccB7I CCANNNNNTGG 1 cut(s) 189
AccII CGCG 1 cut(s) 852
AciI CCGC 2 cut(s) 227, 852
AclWI GGATC 3 cut(s) 39, 469, 808
AcoI YGGCCR 2 cut(s) 505, 623
AcsI RAATTY 2 cut(s) 237, 305
AfaI GTAC 3 cut(s) 765, 785, 943
AfeI AGCGCT 1 cut(s) 352
AfiI CCNNNNNNNGG 3 cut(s) 189, 509, 903
AgsI TTSAA 3 cut(s) 236, 329, 832
AhlI ACTAGT 1 cut(s) 58
AjiI CACGTC 1 cut(s) 565
AjnI CCWGG 2 cut(s) 502, 625
AluBI AGCT 7 cut(s) 46, 197, 545, 746, 759, 821, 947
AluI AGCT 7 cut(s) 46, 197, 545, 746, 759, 821, 947
Alw26I GTCTC 2 cut(s) 49, 140
AlwI GGATC 3 cut(s) 39, 469, 808
Ama87I CYCGRG 1 cut(s) 902
Aor51HI AGCGCT 1 cut(s) 352
AoxI GGCC 4 cut(s) 505, 623, 724, 734
ApoI RAATTY 2 cut(s) 237, 305
AseI ATTAAT 1 cut(s) 282
AspLEI GCGC 4 cut(s) 290, 353, 839, 852
AspS9I GGNCC 3 cut(s) 500, 681, 734
AsuC2I CCSGG 3 cut(s) 733, 903, 904
AsuHPI GGTGA 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 2 cut(s) 500, 681
BalI TGGCCA 2 cut(s) 507, 625
BarI GAAGNNNNNNTAC 2 cut(s) 451, 483
BauI CACGAG 1 cut(s) 979
BbsI GAAGAC 2 cut(s) 567, 917
BccI CCATC 4 cut(s) 125, 164, 371, 461
BciT130I CCWGG 2 cut(s) 504, 627
BcnI CCSGG 3 cut(s) 733, 903, 904
BcoDI GTCTC 2 cut(s) 49, 140
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 3 cut(s) 59, 158, 645
BfmI CTRYAG 1 cut(s) 426
BfoI RGCGCY 1 cut(s) 354
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 5 cut(s) 504, 627, 733, 903, 904
Bme18I GGWCC 2 cut(s) 500, 681
BmeT110I CYCGRG 1 cut(s) 902
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 3 cut(s) 500, 681, 734
BmiI GGNNCC 2 cut(s) 181, 682
BmrFI CCNGG 5 cut(s) 504, 627, 733, 903, 904
BpiI GAAGAC 2 cut(s) 567, 917
BpuEI CTTGAG 1 cut(s) 607
BpuMI CCSGG 3 cut(s) 733, 903, 904
BsaAI YACGTR 1 cut(s) 611
BsaBI GATNNNNATC 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 6 cut(s) 49, 478, 508, 787, 902, 937
BsaWI WCCGGW 1 cut(s) 750
BsaXI ACNNNNNCTCC 4 cut(s) 315, 345, 838, 868
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 509, 903
Bse1I ACTGG 1 cut(s) 439
Bse3DI GCAATG 1 cut(s) 447
Bse8I GATNNNNATC 1 cut(s) 386
BseBI CCWGG 2 cut(s) 504, 627
BseDI CCNNGG 6 cut(s) 49, 478, 508, 787, 902, 937
BseGI GGATG 1 cut(s) 893
BseJI GATNNNNATC 1 cut(s) 386
BseLI CCNNNNNNNGG 3 cut(s) 189, 509, 903
BseMI GCAATG 1 cut(s) 447
BseMII CTCAG 4 cut(s) 135, 254, 485, 774
BseNI ACTGG 1 cut(s) 439
BseRI GAGGAG 1 cut(s) 165
BseYI CCCAGC 1 cut(s) 839
Bsh1236I CGCG 1 cut(s) 852
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 4 cut(s) 507, 625, 726, 736
BsiEI CGRYCG 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 3 cut(s) 732, 751, 903
BslFI GGGAC 1 cut(s) 953
BslI CCNNNNNNNGG 3 cut(s) 189, 509, 903
BsmAI GTCTC 2 cut(s) 49, 140
BsmFI GGGAC 1 cut(s) 953
BsnI GGCC 4 cut(s) 507, 625, 726, 736
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 902
Bsp143I GATC 5 cut(s) 31, 217, 387, 474, 800
Bsp19I CCATGG 3 cut(s) 508, 787, 937
BspACI CCGC 2 cut(s) 227, 852
BspANI GGCC 4 cut(s) 507, 625, 726, 736
BspCNI CTCAG 4 cut(s) 134, 255, 484, 773
BspFNI CGCG 1 cut(s) 852
BspLI GGNNCC 2 cut(s) 181, 682
BspPI GGATC 3 cut(s) 39, 469, 808
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 447
BsrI ACTGG 1 cut(s) 439
BssECI CCNNGG 6 cut(s) 49, 478, 508, 787, 902, 937
BssMI GATC 5 cut(s) 31, 217, 387, 474, 800
BssSI CACGAG 1 cut(s) 979
BssT1I CCWWGG 5 cut(s) 49, 478, 508, 787, 937
Bst2BI CACGAG 1 cut(s) 979
Bst2UI CCWGG 2 cut(s) 504, 627
Bst4CI ACNGT 2 cut(s) 80, 556
Bst6I CTCTTC 1 cut(s) 628
BstBAI YACGTR 1 cut(s) 611
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 4 cut(s) 121, 263, 471, 760
BstDSI CCRYGG 3 cut(s) 508, 787, 937
BstF5I GGATG 1 cut(s) 893
BstFNI CGCG 1 cut(s) 852
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 4 cut(s) 290, 353, 839, 852
BstKTI GATC 5 cut(s) 34, 220, 390, 477, 803
BstMAI GTCTC 2 cut(s) 49, 140
BstMBI GATC 5 cut(s) 31, 217, 387, 474, 800
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 1 cut(s) 953
BstNI CCWGG 2 cut(s) 504, 627
BstSCI CCNGG 5 cut(s) 502, 625, 731, 901, 902
BstSFI CTRYAG 1 cut(s) 426
BstSNI TACGTA 1 cut(s) 611
BstUI CGCG 1 cut(s) 852
BstV2I GAAGAC 2 cut(s) 567, 917
BstX2I RGATCY 3 cut(s) 31, 474, 800
BstXI CCANNNNNNTGG 1 cut(s) 485
BstYI RGATCY 3 cut(s) 31, 474, 800
BsuRI GGCC 4 cut(s) 507, 625, 726, 736
BtgI CCRYGG 3 cut(s) 508, 787, 937
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 1 cut(s) 893
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 1 cut(s) 199
CfoI GCGC 4 cut(s) 290, 353, 839, 852
Cfr13I GGNCC 3 cut(s) 500, 681, 734
Cfr9I CCCGGG 1 cut(s) 902
Csp6I GTAC 3 cut(s) 764, 784, 942
CviAII CATG 6 cut(s) 87, 509, 621, 788, 938, 972
CviQI GTAC 3 cut(s) 764, 784, 942
DdeI CTNAG 4 cut(s) 121, 263, 471, 760
DpnI GATC 5 cut(s) 33, 219, 389, 476, 802
DpnII GATC 5 cut(s) 31, 217, 387, 474, 800
EaeI YGGCCR 2 cut(s) 505, 623
Eam1104I CTCTTC 1 cut(s) 628
EarI CTCTTC 1 cut(s) 628
Eco105I TACGTA 1 cut(s) 611
Eco130I CCWWGG 5 cut(s) 49, 478, 508, 787, 937
Eco147I AGGCCT 1 cut(s) 726
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 2 cut(s) 500, 681
Eco47III AGCGCT 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 902
EcoRII CCWGG 2 cut(s) 502, 625
EcoT14I CCWWGG 5 cut(s) 49, 478, 508, 787, 937
ErhI CCWWGG 5 cut(s) 49, 478, 508, 787, 937
FaeI CATG 6 cut(s) 90, 512, 624, 791, 941, 975
FaqI GGGAC 1 cut(s) 953
FatI CATG 6 cut(s) 86, 508, 620, 787, 937, 971
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 1 cut(s) 880
Fsp4HI GCNGC 1 cut(s) 228
FspBI CTAG 3 cut(s) 59, 158, 645
GlaI GCGC 4 cut(s) 289, 352, 838, 851
GluI GCNGC 1 cut(s) 228
GsaI CCCAGC 1 cut(s) 843
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 4 cut(s) 507, 625, 726, 736
HapII CCGG 3 cut(s) 732, 751, 903
HhaI GCGC 4 cut(s) 290, 353, 839, 852
Hin1II CATG 6 cut(s) 90, 512, 624, 791, 941, 975
Hin6I GCGC 4 cut(s) 288, 351, 837, 850
HinP1I GCGC 4 cut(s) 288, 351, 837, 850
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 3 cut(s) 92, 382, 666
HpaI GTTAAC 1 cut(s) 409
HpaII CCGG 3 cut(s) 732, 751, 903
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 3 cut(s) 409, 500, 568
Hpy188I TCNGA 8 cut(s) 97, 124, 211, 260, 369, 387, 474, 671
Hpy188III TCNNGA 4 cut(s) 233, 356, 616, 981
Hpy8I GTNNAC 3 cut(s) 409, 500, 568
HpyAV CCTTC 1 cut(s) 908
HpyCH4III ACNGT 2 cut(s) 80, 556
HpyCH4IV ACGT 5 cut(s) 27, 564, 570, 610, 782
HpyCH4V TGCA 5 cut(s) 65, 318, 447, 582, 927
HpyF10VI GCNNNNNNNGC 1 cut(s) 953
HpyF3I CTNAG 4 cut(s) 121, 263, 471, 760
HpySE526I ACGT 5 cut(s) 27, 564, 570, 610, 782
Hsp92II CATG 6 cut(s) 90, 512, 624, 791, 941, 975
HspAI GCGC 4 cut(s) 288, 351, 837, 850
KspAI GTTAAC 1 cut(s) 409
Kzo9I GATC 5 cut(s) 31, 217, 387, 474, 800
LmnI GCTCC 3 cut(s) 51, 358, 542
MaeI CTAG 3 cut(s) 59, 158, 645
MaeII ACGT 5 cut(s) 27, 564, 570, 610, 782
MaeIII GTNAC 2 cut(s) 271, 778
MalI GATC 5 cut(s) 33, 219, 389, 476, 802
MboI GATC 5 cut(s) 31, 217, 387, 474, 800
MboII GAAGA 6 cut(s) 459, 572, 645, 795, 798, 922
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 3 cut(s) 31, 474, 800
MlsI TGGCCA 2 cut(s) 507, 625
MluCI AATT 8 cut(s) 38, 165, 184, 237, 305, 418, 577, 826
MluNI TGGCCA 2 cut(s) 507, 625
MmeI TCCRAC 2 cut(s) 283, 694
MnlI CCTC 7 cut(s) 143, 146, 584, 629, 655, 808, 840
Mox20I TGGCCA 2 cut(s) 507, 625
MscI TGGCCA 2 cut(s) 507, 625
MseI TTAA 3 cut(s) 36, 282, 408
MslI CAYNNNNRTG 1 cut(s) 869
Msp20I TGGCCA 2 cut(s) 507, 625
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 3 cut(s) 732, 751, 903
MspR9I CCNGG 5 cut(s) 504, 627, 733, 903, 904
MunI CAATTG 1 cut(s) 184
MvaI CCWGG 2 cut(s) 504, 627
MvnI CGCG 1 cut(s) 852
MwoI GCNNNNNNNGC 1 cut(s) 953
NciI CCSGG 3 cut(s) 733, 903, 904
NcoI CCATGG 3 cut(s) 508, 787, 937
NdeII GATC 5 cut(s) 31, 217, 387, 474, 800
NlaIII CATG 6 cut(s) 90, 512, 624, 791, 941, 975
NlaIV GGNNCC 2 cut(s) 181, 682
NmuCI GTSAC 2 cut(s) 271, 778
PceI AGGCCT 1 cut(s) 726
PfeI GAWTC 3 cut(s) 92, 382, 666
PflMI CCANNNNNTGG 1 cut(s) 189
PkrI GCNGC 1 cut(s) 229
Ple19I CGATCG 1 cut(s) 220
Ppu21I YACGTR 1 cut(s) 611
PshBI ATTAAT 1 cut(s) 282
PsiI TTATAA 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 502, 625
PspFI CCCAGC 1 cut(s) 839
PspGI CCWGG 2 cut(s) 502, 625
PspN4I GGNNCC 2 cut(s) 181, 682
PspPI GGNCC 3 cut(s) 500, 681, 734
PsuI RGATCY 3 cut(s) 31, 474, 800
PvuI CGATCG 1 cut(s) 220
RsaI GTAC 3 cut(s) 765, 785, 943
RsaNI GTAC 3 cut(s) 764, 784, 942
RseI CAYNNNNRTG 1 cut(s) 869
SaqAI TTAA 3 cut(s) 36, 282, 408
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 5 cut(s) 31, 217, 387, 474, 800
Sau96I GGNCC 3 cut(s) 500, 681, 734
ScrFI CCNGG 5 cut(s) 504, 627, 733, 903, 904
SfcI CTRYAG 1 cut(s) 426
SinI GGWCC 2 cut(s) 500, 681
SmaI CCCGGG 1 cut(s) 904
SmiMI CAYNNNNRTG 1 cut(s) 869
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
SnaBI TACGTA 1 cut(s) 611
SpeI ACTAGT 1 cut(s) 58
Sse9I AATT 8 cut(s) 38, 165, 184, 237, 305, 418, 577, 826
SseBI AGGCCT 1 cut(s) 726
SsiI CCGC 2 cut(s) 227, 852
SspI AATATT 1 cut(s) 18
SspMI CTAG 3 cut(s) 59, 158, 645
StuI AGGCCT 1 cut(s) 726
StyD4I CCNGG 5 cut(s) 502, 625, 731, 901, 902
StyI CCWWGG 5 cut(s) 49, 478, 508, 787, 937
TaaI ACNGT 2 cut(s) 80, 556
TaiI ACGT 5 cut(s) 30, 567, 573, 613, 785
TaqI TCGA 3 cut(s) 220, 493, 717
TasI AATT 8 cut(s) 38, 165, 184, 237, 305, 418, 577, 826
TatI WGTACW 1 cut(s) 763
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 3 cut(s) 92, 382, 666
Tru1I TTAA 3 cut(s) 36, 282, 408
Tru9I TTAA 3 cut(s) 36, 282, 408
TscAI CASTG 1 cut(s) 561
TseFI GTSAC 2 cut(s) 271, 778
Tsp45I GTSAC 2 cut(s) 271, 778
TspDTI ATGAA 3 cut(s) 375, 384, 882
TspGWI ACGGA 1 cut(s) 336
TspMI CCCGGG 1 cut(s) 902
TspRI CASTG 1 cut(s) 561
Van91I CCANNNNNTGG 1 cut(s) 189
VpaK11BI GGWCC 2 cut(s) 500, 681
VspI ATTAAT 1 cut(s) 282
XapI RAATTY 2 cut(s) 237, 305
XcmI CCANNNNNNNNNTGG 1 cut(s) 794
XmaI CCCGGG 1 cut(s) 902
XspI CTAG 3 cut(s) 59, 158, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.