Rh1BG079900

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002703
Physical Location & Seq
Reverse (-)
12500716 .. 12503130
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG079900.1

Sequence Viewer

Length: 1431 bp
ATGGAGGGTCGAATACTTGCTACGATCTTTTTGTTCATGATCATTCTGGTTGCTTCACCTTCACCAAGTTCATGTGCCAGACTTAATGGTTTTCCCACAACATATACTTTAAGAGACGATGGTGCCATTGGTTATTATGGTGATCAAGTTGCTGCTGCGACATCAAATTCATTCCATACTAAGGAGTATGGTGATGTTTTTGCAACCCCAAATACATTCAAAGAGTATTATCGTCCCCTTGCAAATCGCCAAACCGATGATGATTTACAAGCATCCCCAGATGCATGGAGATCACTTGCAAAAAGCGAATTCAATGTGATGAATTATGGCGCTGTTGGGAATGGCCAAGTTGATGATACACAAGCCTTTCTAAAAGCATGGGGAGATGTTTGTGGATCCACTCAAGGCATCCCTACACTTATTATACCAAAGGGAAACACTTTCTTGCTCAATTCTGTGGAGTTTAAAGGGCCTTGCAGGGCTGCAAGTGTAAATTTCCAGCTAAGTGGGAACATTGTTGCACCAAATAATACGAATGCTTGGAAAGACAAGGACCAATGGGTTCAGTTTGATGATGTTCAAGGTCTGATAATCAATGGAGGAGGTCAAATTGATGGTCAAGGTCAGGTTTGGTGGAAAGTTTGCGACACTACAAATTGCCAACGACCAACTGCTCTTCATATTAGCAAGTGTGATGGTCTCCGCTTGAGTAATATTACACATATTAATAGTGCGAGAAACCATATAAGCATCACCGGCTGCAATGGCGTTCAAATTTCTGATATCCTTATTAGGGCTCCTGATGTAAGTCCAAACACTGATGGAATTGACATCTCTGCATCAACCAATATTAACATTCACAATTCTTTTATAGGCACTGGTGACGACTGTATTGCAATCATTTCTGGTTCATCTCATATCAACATTACTGATGTTATGTGTGGTCCAGGCCATGGTATAAGTGTTGGAAGTCTTGGTAAGCAAGGTTCTTATAGCACAGTGGAAGATGTGCAAGTGAGAAACTGCACCTTCAACGGAACACAAAATGGAGCAAGAATCAAGACATGGCAAGGCGGGTCAGGGTATGCTAGGAACATCAGTTTTGAGGATATCATAATTCAAGCAGCCAAGAACCCTATCATTATAGACCAGAAGTATACTGATAATCTCTCTACTGGAACTGGAACTGGAACCCTGCTTGACCAAGAAAATGCTGTACAAGTGAGCGATGTGACATTCCGTAACATTCGGGGATCTGTTGCTGGGGATACAGCCATTACTTTGGACTGTGACGATCAAATTGGTTGCAAAAACATTGTAATGGATCACATAGACTTAACTTCATCTGTTCCTGGCAAGAAGGTTTCTGCCCACTGCAAGAACGTTAAAGGATTATCTACTTTATTATCTCCCAGTGTGTCATGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

476

Amino Acids

50.98

Weight (kDa)

5.34

Isoelectric Point (pI)

28.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 135 - 463 1.8e-91 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 953
AccI GTMKAC 1 cut(s) 1157
AciI CCGC 2 cut(s) 703, 1074
AclI AACGTT 1 cut(s) 1383
AclWI GGATC 4 cut(s) 390, 403, 1261, 1332
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 4 cut(s) 166, 308, 493, 774
AfaI GTAC 1 cut(s) 1218
AfiI CCNNNNNNNGG 3 cut(s) 181, 793, 953
AgsI TTSAA 6 cut(s) 220, 313, 581, 773, 1033, 1121
AjnI CCWGG 2 cut(s) 946, 1351
AluBI AGCT 1 cut(s) 502
AluI AGCT 1 cut(s) 502
Alw26I GTCTC 2 cut(s) 108, 704
AlwI GGATC 4 cut(s) 390, 403, 1261, 1332
AoxI GGCC 3 cut(s) 343, 470, 949
ApeKI GCWGC 5 cut(s) 152, 155, 482, 759, 1124
ApoI RAATTY 4 cut(s) 166, 308, 493, 774
ArsI GACNNNNNNTTYG 2 cut(s) 601, 633
AseI ATTAAT 1 cut(s) 726
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 3 cut(s) 470, 553, 944
AsuHPI GGTGA 6 cut(s) 48, 54, 152, 203, 745, 893
AvaII GGWCC 2 cut(s) 553, 944
BalI TGGCCA 1 cut(s) 345
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 799
BbvI GCAGC 5 cut(s) 139, 142, 469, 746, 1136
BccI CCATC 4 cut(s) 113, 608, 689, 815
BcgI CGANNNNNNTGC 2 cut(s) 875, 909
BciT130I CCWGG 2 cut(s) 948, 1353
BciVI GTATCC 1 cut(s) 1261
BclI TGATCA 2 cut(s) 39, 142
BcoDI GTCTC 2 cut(s) 108, 704
BfaI CTAG 1 cut(s) 1089
BfoI RGCGCY 1 cut(s) 333
BfuI GTATCC 1 cut(s) 1261
BisI GCNGC 5 cut(s) 153, 156, 483, 760, 1125
BlsI GCNGC 5 cut(s) 154, 157, 484, 761, 1126
Bme1390I CCNGG 2 cut(s) 948, 1353
Bme18I GGWCC 2 cut(s) 553, 944
BmgT120I GGNCC 3 cut(s) 470, 553, 944
BmiI GGNNCC 4 cut(s) 124, 397, 798, 1192
BmrFI CCNGG 2 cut(s) 948, 1353
BmrI ACTGGG 1 cut(s) 1407
BmsI GCATC 5 cut(s) 271, 281, 417, 759, 848
BmuI ACTGGG 1 cut(s) 1407
BpuEI CTTGAG 2 cut(s) 387, 727
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 1 cut(s) 952
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 793, 953
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 5 cut(s) 883, 1180, 1186, 1192, 1413
Bse3DI GCAATG 1 cut(s) 769
BseBI CCWGG 2 cut(s) 948, 1353
BseDI CCNNGG 1 cut(s) 952
BseGI GGATG 2 cut(s) 272, 408
BseLI CCNNNNNNNGG 3 cut(s) 181, 793, 953
BseMI GCAATG 1 cut(s) 769
BseNI ACTGG 5 cut(s) 883, 1180, 1186, 1192, 1413
BseRI GAGGAG 1 cut(s) 615
BseXI GCAGC 5 cut(s) 139, 142, 469, 746, 1136
BseYI CCCAGC 1 cut(s) 1262
BsgI GTGCAG 1 cut(s) 1009
BshFI GGCC 3 cut(s) 345, 472, 951
BshNI GGYRCC 1 cut(s) 122
BsiSI CCGG 1 cut(s) 756
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 3 cut(s) 181, 793, 953
BsmAI GTCTC 2 cut(s) 108, 704
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 219
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 3 cut(s) 345, 472, 951
Bso31I GGTCTC 1 cut(s) 704
Bsp1286I GDGCHC 1 cut(s) 799
Bsp1407I TGTACA 1 cut(s) 1216
Bsp143I GATC 8 cut(s) 24, 39, 142, 290, 395, 1253, 1294, 1324
Bsp19I CCATGG 1 cut(s) 952
BspACI CCGC 2 cut(s) 703, 1074
BspANI GGCC 3 cut(s) 345, 472, 951
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 4 cut(s) 124, 397, 798, 1192
BspPI GGATC 4 cut(s) 390, 403, 1261, 1332
BspQI GCTCTTC 1 cut(s) 681
BspT107I GGYRCC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 769
BsrFI RCCGGY 1 cut(s) 755
BsrGI TGTACA 1 cut(s) 1216
BsrI ACTGG 5 cut(s) 883, 1180, 1186, 1192, 1413
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 1 cut(s) 952
BssMI GATC 8 cut(s) 24, 39, 142, 290, 395, 1253, 1294, 1324
BssNAI GTATAC 1 cut(s) 1158
BssT1I CCWWGG 1 cut(s) 952
Bst1107I GTATAC 1 cut(s) 1158
Bst2UI CCWGG 2 cut(s) 948, 1353
Bst4CI ACNGT 3 cut(s) 890, 1000, 1289
Bst6I CTCTTC 1 cut(s) 681
BstAUI TGTACA 1 cut(s) 1216
BstDEI CTNAG 2 cut(s) 180, 503
BstDSI CCRYGG 1 cut(s) 952
BstF5I GGATG 2 cut(s) 272, 408
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 8 cut(s) 27, 42, 145, 293, 398, 1256, 1297, 1327
BstMAI GTCTC 2 cut(s) 108, 704
BstMBI GATC 8 cut(s) 24, 39, 142, 290, 395, 1253, 1294, 1324
BstMWI GCNNNNNNNGC 2 cut(s) 756, 765
BstNI CCWGG 2 cut(s) 948, 1353
BstSCI CCNGG 2 cut(s) 946, 1351
BstV1I GCAGC 5 cut(s) 139, 142, 469, 746, 1136
BstX2I RGATCY 2 cut(s) 395, 1253
BstXI CCANNNNNNTGG 3 cut(s) 285, 506, 1282
BstYI RGATCY 2 cut(s) 395, 1253
BstZ17I GTATAC 1 cut(s) 1158
BsuI GTATCC 1 cut(s) 1261
BsuRI GGCC 3 cut(s) 345, 472, 951
BtgI CCRYGG 1 cut(s) 952
BtgZI GCGATG 1 cut(s) 1242
BtsCI GGATG 2 cut(s) 272, 408
BtsI GCAGTG 1 cut(s) 1372
BtsIMutI CAGTG 5 cut(s) 816, 876, 1005, 1372, 1420
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 3 cut(s) 470, 553, 944
Csp6I GTAC 1 cut(s) 1217
CviAII CATG 7 cut(s) 37, 72, 285, 378, 953, 1065, 1422
CviQI GTAC 1 cut(s) 1217
DdeI CTNAG 2 cut(s) 180, 503
DpnI GATC 8 cut(s) 26, 41, 144, 292, 397, 1255, 1296, 1326
DpnII GATC 8 cut(s) 24, 39, 142, 290, 395, 1253, 1294, 1324
DraI TTTAAA 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
Eco130I CCWWGG 1 cut(s) 952
Eco24I GRGCYC 1 cut(s) 799
Eco31I GGTCTC 1 cut(s) 704
Eco32I GATATC 2 cut(s) 784, 1111
Eco47I GGWCC 2 cut(s) 553, 944
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 308
EcoRII CCWGG 2 cut(s) 946, 1351
EcoRV GATATC 2 cut(s) 784, 1111
EcoT14I CCWWGG 1 cut(s) 952
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 952
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 7 cut(s) 40, 75, 288, 381, 956, 1068, 1425
FaqI GGGAC 1 cut(s) 219
FatI CATG 7 cut(s) 36, 71, 284, 377, 952, 1064, 1421
FauI CCCGC 1 cut(s) 1067
FbaI TGATCA 2 cut(s) 39, 142
FblI GTMKAC 1 cut(s) 1157
Fnu4HI GCNGC 5 cut(s) 153, 156, 483, 760, 1125
FokI GGATG 2 cut(s) 259, 395
FriOI GRGCYC 1 cut(s) 799
Fsp4HI GCNGC 5 cut(s) 153, 156, 483, 760, 1125
FspBI CTAG 1 cut(s) 1089
GlaI GCGC 1 cut(s) 331
GluI GCNGC 5 cut(s) 153, 156, 483, 760, 1125
GsaI CCCAGC 1 cut(s) 1266
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 3 cut(s) 345, 472, 951
HapII CCGG 1 cut(s) 756
HhaI GCGC 1 cut(s) 332
Hin1II CATG 7 cut(s) 40, 75, 288, 381, 956, 1068, 1425
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 1 cut(s) 1056
HpaII CCGG 1 cut(s) 756
HphI GGTGA 6 cut(s) 48, 54, 152, 203, 745, 893
Hpy166II GTNNAC 1 cut(s) 1158
Hpy188I TCNGA 2 cut(s) 588, 781
Hpy188III TCNNGA 3 cut(s) 37, 800, 1060
Hpy8I GTNNAC 1 cut(s) 1158
HpyAV CCTTC 3 cut(s) 69, 1039, 1354
HpyCH4III ACNGT 3 cut(s) 890, 1000, 1289
HpyCH4IV ACGT 1 cut(s) 1383
HpyF10VI GCNNNNNNNGC 2 cut(s) 756, 765
HpyF3I CTNAG 2 cut(s) 180, 503
HpySE526I ACGT 1 cut(s) 1383
Hsp92II CATG 7 cut(s) 40, 75, 288, 381, 956, 1068, 1425
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 2 cut(s) 39, 142
Kzo9I GATC 8 cut(s) 24, 39, 142, 290, 395, 1253, 1294, 1324
LguI GCTCTTC 1 cut(s) 681
LmnI GCTCC 2 cut(s) 802, 1049
Lsp1109I GCAGC 5 cut(s) 139, 142, 469, 746, 1136
LweI GCATC 5 cut(s) 271, 281, 417, 759, 848
MaeI CTAG 1 cut(s) 1089
MaeII ACGT 1 cut(s) 1383
MaeIII GTNAC 4 cut(s) 881, 1231, 1241, 1289
MalI GATC 8 cut(s) 26, 41, 144, 292, 397, 1255, 1296, 1326
MboI GATC 8 cut(s) 24, 39, 142, 290, 395, 1253, 1294, 1324
MboII GAAGA 2 cut(s) 668, 1016
MflI RGATCY 2 cut(s) 395, 1253
MhlI GDGCHC 1 cut(s) 799
MlsI TGGCCA 1 cut(s) 345
MluNI TGGCCA 1 cut(s) 345
MmeI TCCRAC 1 cut(s) 946
MnlI CCTC 3 cut(s) 593, 596, 1099
Mox20I TGGCCA 1 cut(s) 345
Mph1103I ATGCAT 1 cut(s) 286
MscI TGGCCA 1 cut(s) 345
MseI TTAA 8 cut(s) 84, 110, 465, 726, 852, 1337, 1386, 1429
MslI CAYNNNNRTG 1 cut(s) 1319
Msp20I TGGCCA 1 cut(s) 345
MspI CCGG 1 cut(s) 756
MspR9I CCNGG 2 cut(s) 948, 1353
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 948, 1353
MwoI GCNNNNNNNGC 2 cut(s) 756, 765
NcoI CCATGG 1 cut(s) 952
NdeII GATC 8 cut(s) 24, 39, 142, 290, 395, 1253, 1294, 1324
NlaIII CATG 7 cut(s) 40, 75, 288, 381, 956, 1068, 1425
NlaIV GGNNCC 4 cut(s) 124, 397, 798, 1192
NmuCI GTSAC 3 cut(s) 881, 1231, 1289
NsiI ATGCAT 1 cut(s) 286
PagI TCATGA 1 cut(s) 36
PciSI GCTCTTC 1 cut(s) 681
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 1 cut(s) 1056
PflMI CCANNNNNTGG 1 cut(s) 953
PkrI GCNGC 5 cut(s) 154, 157, 484, 761, 1126
PshBI ATTAAT 1 cut(s) 726
Psp1406I AACGTT 1 cut(s) 1383
Psp6I CCWGG 2 cut(s) 946, 1351
PspFI CCCAGC 1 cut(s) 1262
PspGI CCWGG 2 cut(s) 946, 1351
PspN4I GGNNCC 4 cut(s) 124, 397, 798, 1192
PspPI GGNCC 3 cut(s) 470, 553, 944
PsrI GAACNNNNNNTAC 2 cut(s) 970, 1002
PsuI RGATCY 2 cut(s) 395, 1253
RsaI GTAC 1 cut(s) 1218
RsaNI GTAC 1 cut(s) 1217
RseI CAYNNNNRTG 1 cut(s) 1319
SapI GCTCTTC 1 cut(s) 681
SaqAI TTAA 8 cut(s) 84, 110, 465, 726, 852, 1337, 1386, 1429
SatI GCNGC 5 cut(s) 153, 156, 483, 760, 1125
Sau3AI GATC 8 cut(s) 24, 39, 142, 290, 395, 1253, 1294, 1324
Sau96I GGNCC 3 cut(s) 470, 553, 944
ScrFI CCNGG 2 cut(s) 948, 1353
SduI GDGCHC 1 cut(s) 799
SfaNI GCATC 5 cut(s) 271, 281, 417, 759, 848
SinI GGWCC 2 cut(s) 553, 944
SmiMI CAYNNNNRTG 1 cut(s) 1319
SmlI CTYRAG 2 cut(s) 402, 706
SmoI CTYRAG 2 cut(s) 402, 706
SsiI CCGC 2 cut(s) 703, 1074
SspI AATATT 2 cut(s) 715, 850
SspMI CTAG 1 cut(s) 1089
StyD4I CCNGG 2 cut(s) 946, 1351
StyI CCWWGG 1 cut(s) 952
TaaI ACNGT 3 cut(s) 890, 1000, 1289
TaiI ACGT 1 cut(s) 1386
TaqI TCGA 1 cut(s) 10
TatI WGTACW 1 cut(s) 1216
TfiI GAWTC 1 cut(s) 1056
Tru1I TTAA 8 cut(s) 84, 110, 465, 726, 852, 1337, 1386, 1429
Tru9I TTAA 8 cut(s) 84, 110, 465, 726, 852, 1337, 1386, 1429
TscAI CASTG 5 cut(s) 823, 883, 1005, 1379, 1420
TseFI GTSAC 3 cut(s) 881, 1231, 1289
TseI GCWGC 5 cut(s) 152, 155, 482, 759, 1124
Tsp45I GTSAC 3 cut(s) 881, 1231, 1289
TspDTI ATGAA 7 cut(s) 25, 60, 159, 335, 668, 900, 1332
TspGWI ACGGA 2 cut(s) 1050, 1229
TspRI CASTG 5 cut(s) 823, 883, 1005, 1379, 1420
Van91I CCANNNNNTGG 1 cut(s) 953
VpaK11BI GGWCC 2 cut(s) 553, 944
VspI ATTAAT 1 cut(s) 726
XapI RAATTY 4 cut(s) 166, 308, 493, 774
XmiI GTMKAC 1 cut(s) 1157
XspI CTAG 1 cut(s) 1089
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.