Rw1G007960

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002703
Physical Location & Seq
Reverse (-)
17061618 .. 17063222
1605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G007960.1

Sequence Viewer

Length: 900 bp
ATGGAGGGTCGAGTACTTGCTACGATCTTTTTGTTCATGATCATTCTGGTTGCTTCACCTTCACCAAGTTCATGTGCCAGACTTAATGGTTTTCCGACAACATATACTTTAAGAGACGATGGTGCCATTGGTTATTATGGTGATCAAGTTGCTGCTGCGACATCAAATTCATTCCATACTAATGAGTATGGTGATGTTTTTGCAACCCCAAATACATTCGAAGAGTATTATCGTCCCCTTGCAAATCGCCAAACCGATGATGATTTACTAGCATCCCCAGATGCATGGAGATCACTTGCAAAAAGCGAATTCAATGTGATGAATTATGGCGCTGTTGGGAATGGCCAAGTTGATGATACACAAGTATCATGGGGAGATGTTTGTGGATCCACTCAAGGCATCCCTACACTTATTATACCAAAGGGCAACACTTTCTTGCTCAATTCTGTGGAGTTTAAAGGGCCTTGCAGGGCTGCAAGTGTAAATTTCCAGCTAAGTGGGAACATTGTTGCACCAAATAATATGAATGCTTGGAAAGACAAGGACCAATGGGTTCAGTTTGATGATGTTCAAGGTCTGATAATCAATGGAGGAGGTCAAATTGATGGTCAAGGTCAGGCTCTTCATATTAGCAAGTGTGATGGTCTCCGCTTGAGTAATATTACACATATTAATAGTGCGAGAAACCATATAAGCATCACCGGCTGCAATGGCGTTCAAATTTCTGATATCCTTATTAGGGCTCCTGATGAAAGTCCAAACACTGATGGAATTGACATCTCTGCATCAACCAATATTAACATTCACAATTCTTTTATAGGCACTGGTACTGGAATCAACTTTCCTCAACACTGTTCTTACATTATTTTTATTTTTTTTGTTGCATGTTGTTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

299

Amino Acids

32.35

Weight (kDa)

4.8

Isoelectric Point (pI)

29.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 132 - 276 3.6e-24 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AciI CCGC 1 cut(s) 649
AclWI GGATC 2 cut(s) 381, 394
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 4 cut(s) 166, 308, 484, 720
AfaI GTAC 2 cut(s) 15, 829
AfiI CCNNNNNNNGG 1 cut(s) 739
AgsI TTSAA 3 cut(s) 313, 572, 719
AluBI AGCT 1 cut(s) 493
AluI AGCT 1 cut(s) 493
Alw26I GTCTC 2 cut(s) 108, 650
AlwI GGATC 2 cut(s) 381, 394
AoxI GGCC 2 cut(s) 343, 461
ApeKI GCWGC 4 cut(s) 152, 155, 473, 705
ApoI RAATTY 4 cut(s) 166, 308, 484, 720
ArsI GACNNNNNNTTYG 2 cut(s) 592, 624
AseI ATTAAT 1 cut(s) 672
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 461, 544
AsuHPI GGTGA 5 cut(s) 48, 54, 152, 203, 691
AsuII TTCGAA 1 cut(s) 219
AvaII GGWCC 1 cut(s) 544
BaeI ACNNNNGTAYC 4 cut(s) 348, 348, 381, 381
BalI TGGCCA 1 cut(s) 345
BamHI GGATCC 1 cut(s) 386
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 745
BbvI GCAGC 4 cut(s) 139, 142, 460, 692
BccI CCATC 4 cut(s) 113, 599, 635, 761
BclI TGATCA 2 cut(s) 39, 142
BcoDI GTCTC 2 cut(s) 108, 650
BfaI CTAG 1 cut(s) 269
BfoI RGCGCY 1 cut(s) 333
BisI GCNGC 4 cut(s) 153, 156, 474, 706
BlsI GCNGC 4 cut(s) 154, 157, 475, 707
BmcAI AGTACT 1 cut(s) 15
Bme18I GGWCC 1 cut(s) 544
BmgT120I GGNCC 2 cut(s) 461, 544
BmiI GGNNCC 3 cut(s) 124, 388, 744
BmsI GCATC 5 cut(s) 271, 281, 408, 705, 794
Bpu14I TTCGAA 1 cut(s) 219
BpuEI CTTGAG 2 cut(s) 378, 673
BsaI GGTCTC 1 cut(s) 650
Bsc4I CCNNNNNNNGG 1 cut(s) 739
Bse118I RCCGGY 1 cut(s) 701
Bse1I ACTGG 2 cut(s) 829, 835
Bse3DI GCAATG 1 cut(s) 715
BseGI GGATG 2 cut(s) 272, 399
BseLI CCNNNNNNNGG 1 cut(s) 739
BseMI GCAATG 1 cut(s) 715
BseNI ACTGG 2 cut(s) 829, 835
BseRI GAGGAG 1 cut(s) 606
BseXI GCAGC 4 cut(s) 139, 142, 460, 692
BshFI GGCC 2 cut(s) 345, 463
BshNI GGYRCC 1 cut(s) 122
BsiSI CCGG 1 cut(s) 702
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 739
BsmAI GTCTC 2 cut(s) 108, 650
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 219
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 2 cut(s) 345, 463
Bso31I GGTCTC 1 cut(s) 650
Bsp119I TTCGAA 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 745
Bsp143I GATC 5 cut(s) 24, 39, 142, 290, 386
BspACI CCGC 1 cut(s) 649
BspANI GGCC 2 cut(s) 345, 463
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 3 cut(s) 124, 388, 744
BspPI GGATC 2 cut(s) 381, 394
BspQI GCTCTTC 1 cut(s) 627
BspT104I TTCGAA 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 715
BsrFI RCCGGY 1 cut(s) 701
BsrI ACTGG 2 cut(s) 829, 835
BssAI RCCGGY 1 cut(s) 701
BssMI GATC 5 cut(s) 24, 39, 142, 290, 386
Bst4CI ACNGT 1 cut(s) 854
Bst6I CTCTTC 2 cut(s) 216, 627
BstBI TTCGAA 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 494
BstF5I GGATG 2 cut(s) 272, 399
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 5 cut(s) 27, 42, 145, 293, 389
BstMAI GTCTC 2 cut(s) 108, 650
BstMBI GATC 5 cut(s) 24, 39, 142, 290, 386
BstMWI GCNNNNNNNGC 2 cut(s) 702, 711
BstNSI RCATGY 1 cut(s) 888
BstV1I GCAGC 4 cut(s) 139, 142, 460, 692
BstX2I RGATCY 1 cut(s) 386
BstXI CCANNNNNNTGG 2 cut(s) 285, 497
BstYI RGATCY 1 cut(s) 386
BsuRI GGCC 2 cut(s) 345, 463
BtsCI GGATG 2 cut(s) 272, 399
BtsIMutI CAGTG 3 cut(s) 762, 822, 850
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 701
Cfr13I GGNCC 2 cut(s) 461, 544
Csp6I GTAC 2 cut(s) 14, 828
CviAII CATG 5 cut(s) 37, 72, 285, 369, 885
CviJI RGCY 7 cut(s) 345, 463, 473, 493, 620, 705, 743
CviKI_1 RGCY 7 cut(s) 345, 463, 473, 493, 620, 705, 743
CviQI GTAC 2 cut(s) 14, 828
DdeI CTNAG 1 cut(s) 494
DpnI GATC 5 cut(s) 26, 41, 144, 292, 388
DpnII GATC 5 cut(s) 24, 39, 142, 290, 386
DraI TTTAAA 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 2 cut(s) 216, 627
EarI CTCTTC 2 cut(s) 216, 627
Eco24I GRGCYC 1 cut(s) 745
Eco31I GGTCTC 1 cut(s) 650
Eco32I GATATC 1 cut(s) 730
Eco47I GGWCC 1 cut(s) 544
EcoO109I RGGNCCY 1 cut(s) 461
EcoRI GAATTC 1 cut(s) 308
EcoRV GATATC 1 cut(s) 730
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 745
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 5 cut(s) 40, 75, 288, 372, 888
FaqI GGGAC 1 cut(s) 219
FatI CATG 5 cut(s) 36, 71, 284, 368, 884
FbaI TGATCA 2 cut(s) 39, 142
Fnu4HI GCNGC 4 cut(s) 153, 156, 474, 706
FokI GGATG 2 cut(s) 259, 386
FriOI GRGCYC 1 cut(s) 745
Fsp4HI GCNGC 4 cut(s) 153, 156, 474, 706
FspBI CTAG 1 cut(s) 269
GlaI GCGC 1 cut(s) 331
GluI GCNGC 4 cut(s) 153, 156, 474, 706
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 2 cut(s) 345, 463
HapII CCGG 1 cut(s) 702
HhaI GCGC 1 cut(s) 332
Hin1II CATG 5 cut(s) 40, 75, 288, 372, 888
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 1 cut(s) 834
HpaII CCGG 1 cut(s) 702
HphI GGTGA 5 cut(s) 48, 54, 152, 203, 691
Hpy188I TCNGA 3 cut(s) 96, 579, 727
Hpy188III TCNNGA 2 cut(s) 37, 746
HpyAV CCTTC 1 cut(s) 69
HpyCH4III ACNGT 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 702, 711
HpyF3I CTNAG 1 cut(s) 494
Hsp92II CATG 5 cut(s) 40, 75, 288, 372, 888
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 2 cut(s) 39, 142
Kzo9I GATC 5 cut(s) 24, 39, 142, 290, 386
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 1 cut(s) 748
Lsp1109I GCAGC 4 cut(s) 139, 142, 460, 692
LweI GCATC 5 cut(s) 271, 281, 408, 705, 794
MaeI CTAG 1 cut(s) 269
MalI GATC 5 cut(s) 26, 41, 144, 292, 388
MboI GATC 5 cut(s) 24, 39, 142, 290, 386
MboII GAAGA 2 cut(s) 233, 614
MflI RGATCY 1 cut(s) 386
MhlI GDGCHC 1 cut(s) 745
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 9 cut(s) 166, 308, 322, 442, 484, 600, 720, 771, 808
MluNI TGGCCA 1 cut(s) 345
MmeI TCCRAC 1 cut(s) 119
MnlI CCTC 3 cut(s) 584, 587, 855
Mox20I TGGCCA 1 cut(s) 345
Mph1103I ATGCAT 1 cut(s) 286
MscI TGGCCA 1 cut(s) 345
MseI TTAA 5 cut(s) 84, 110, 456, 672, 798
MslI CAYNNNNRTG 1 cut(s) 180
Msp20I TGGCCA 1 cut(s) 345
MspI CCGG 1 cut(s) 702
Mva1269I GAATGC 1 cut(s) 532
MwoI GCNNNNNNNGC 2 cut(s) 702, 711
NdeII GATC 5 cut(s) 24, 39, 142, 290, 386
NlaIII CATG 5 cut(s) 40, 75, 288, 372, 888
NlaIV GGNNCC 3 cut(s) 124, 388, 744
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 888
NspV TTCGAA 1 cut(s) 219
PagI TCATGA 1 cut(s) 36
PciSI GCTCTTC 1 cut(s) 627
PctI GAATGC 1 cut(s) 532
PfeI GAWTC 1 cut(s) 834
PkrI GCNGC 4 cut(s) 154, 157, 475, 707
PshBI ATTAAT 1 cut(s) 672
PspN4I GGNNCC 3 cut(s) 124, 388, 744
PspPI GGNCC 2 cut(s) 461, 544
PsuI RGATCY 1 cut(s) 386
RsaI GTAC 2 cut(s) 15, 829
RsaNI GTAC 2 cut(s) 14, 828
RseI CAYNNNNRTG 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 5 cut(s) 84, 110, 456, 672, 798
SatI GCNGC 4 cut(s) 153, 156, 474, 706
Sau3AI GATC 5 cut(s) 24, 39, 142, 290, 386
Sau96I GGNCC 2 cut(s) 461, 544
ScaI AGTACT 1 cut(s) 15
SduI GDGCHC 1 cut(s) 745
SetI ASST 5 cut(s) 61, 495, 577, 598, 616
SfaNI GCATC 5 cut(s) 271, 281, 408, 705, 794
SfuI TTCGAA 1 cut(s) 219
SinI GGWCC 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 393, 652
SmoI CTYRAG 2 cut(s) 393, 652
Sse9I AATT 9 cut(s) 166, 308, 322, 442, 484, 600, 720, 771, 808
SsiI CCGC 1 cut(s) 649
SspI AATATT 2 cut(s) 661, 796
SspMI CTAG 1 cut(s) 269
TaaI ACNGT 1 cut(s) 854
TaqI TCGA 2 cut(s) 10, 219
TasI AATT 9 cut(s) 166, 308, 322, 442, 484, 600, 720, 771, 808
TatI WGTACW 1 cut(s) 13
TfiI GAWTC 1 cut(s) 834
Tru1I TTAA 5 cut(s) 84, 110, 456, 672, 798
Tru9I TTAA 5 cut(s) 84, 110, 456, 672, 798
TscAI CASTG 3 cut(s) 769, 829, 857
TseI GCWGC 4 cut(s) 152, 155, 473, 705
TspDTI ATGAA 7 cut(s) 25, 60, 159, 335, 539, 614, 765
TspRI CASTG 3 cut(s) 769, 829, 857
VpaK11BI GGWCC 1 cut(s) 544
VspI ATTAAT 1 cut(s) 672
XapI RAATTY 4 cut(s) 166, 308, 484, 720
XceI RCATGY 1 cut(s) 888
XspI CTAG 1 cut(s) 269
ZrmI AGTACT 1 cut(s) 15
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.