RLG00000007091

glycoside hydrolase family 28 protein polygalacturonase (pectinase) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002703
Physical Location & Seq
Reverse (-)
13515438 .. 13519397
3960 bp
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UTR
Exon/CDS
Intron
RLM00000007091

Sequence Viewer

Length: 831 bp
ATGCACGGCGTCGTGCAAATTATTTTAAAAATCTCGATTTTGATTGCTGAATTTTCATGCAGGGTCTGTTTGGATGATGACGATGAGTCTTCGATGGCTCTTGAAATTCGTAAATGTGATAATCTCCGACAGAGTGGTGTGACATATATTAATAGCGCAAAAGCCCATATAAAATTCTATGGGTGCAACAGAGTTCAAGTTTCTGGTCTGCTTATTAGCGCTCCCGATGAAAGTCCGAACACAGATGGGATTCAGATCATTTCATCAACTAATGTTAACATTGACAATTCTGCTATAGAAACTGGTGATGACTGCATTACCATCAGTAGTGGTTCTTCTCAGATCCAAGTTAGTGGTGTTCGATGTGGACCTGGCCATGGTATAAGCATTGGAAGTCTTGGGAAAAATAGACCTTACGCCACAGTGGAAGACGTGAAAGTGAGAAATTGCACCTTAAGAGGAACACAAAATGGACTACGTATCAAGACATGGCCAGGTGGAAGAGGTTATGCTAGGAATATCACATATGAGGGAATCCGACTTATAGGGTCCAAGAACCCTGTCATAATAAACCAGCACTATGTCGATAAAGGCTTGTCCGGGCCAAAAGAAGCTGACCGGAAAAGCTCAGTACAAGTGAGTAATGTGACGTACCATGGAGTGTTTGGATCTTCTGCCAATGAGGTAGCTATCAATTTGAACTGCGCTGGGGGGAGGCGCGGGCGACAACATTACAATCGACAACATTCACATTACTTCATCCCTTCCAGGGCAGAAGACTTTTGCCTACTGCAACAATGCCCATGGTACAGCTACACTTGCTATCCCTAA

Protein Analysis

277

Amino Acids

30.23

Weight (kDa)

8.6

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 29 - 239 1.5e-64 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 720
AciI CCGC 1 cut(s) 720
AclWI GGATC 2 cut(s) 337, 676
AcoI YGGCCR 2 cut(s) 373, 491
AcsI RAATTY 3 cut(s) 50, 105, 173
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 3 cut(s) 633, 653, 809
AfeI AGCGCT 1 cut(s) 220
AfiI CCNNNNNNNGG 2 cut(s) 377, 769
AflII CTTAAG 1 cut(s) 454
AgsI TTSAA 3 cut(s) 104, 197, 700
AhdI GACNNNNNGTC 1 cut(s) 85
AjiI CACGTC 1 cut(s) 433
AjnI CCWGG 3 cut(s) 370, 493, 767
AluBI AGCT 4 cut(s) 614, 627, 689, 813
AluI AGCT 4 cut(s) 614, 627, 689, 813
AlwI GGATC 2 cut(s) 337, 676
AlwNI CAGNNNCTG 1 cut(s) 66
Aor51HI AGCGCT 1 cut(s) 220
AoxI GGCC 3 cut(s) 373, 491, 602
ApoI RAATTY 3 cut(s) 50, 105, 173
AseI ATTAAT 1 cut(s) 150
AspLEI GCGC 4 cut(s) 158, 221, 707, 720
AspS9I GGNCC 3 cut(s) 368, 549, 602
AsuC2I CCSGG 1 cut(s) 601
AsuHPI GGTGA 1 cut(s) 317
AvaII GGWCC 2 cut(s) 368, 549
BalI TGGCCA 2 cut(s) 375, 493
BarI GAAGNNNNNNTAC 2 cut(s) 319, 351
BbsI GAAGAC 3 cut(s) 81, 435, 783
BccI CCATC 3 cut(s) 88, 239, 329
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 3 cut(s) 372, 495, 769
BcnI CCSGG 1 cut(s) 601
BfaI CTAG 1 cut(s) 513
BfmI CTRYAG 1 cut(s) 294
BfoI RGCGCY 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 454
Bme1390I CCNGG 4 cut(s) 372, 495, 601, 769
Bme18I GGWCC 2 cut(s) 368, 549
BmeRI GACNNNNNGTC 1 cut(s) 85
BmgBI CACGTC 1 cut(s) 433
BmgT120I GGNCC 3 cut(s) 368, 549, 602
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 4 cut(s) 372, 495, 601, 769
BpiI GAAGAC 3 cut(s) 81, 435, 783
BpuMI CCSGG 1 cut(s) 601
BsaAI YACGTR 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 254
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 4 cut(s) 376, 655, 768, 803
BsaWI WCCGGW 1 cut(s) 618
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 769
Bse1I ACTGG 1 cut(s) 307
Bse8I GATNNNNATC 1 cut(s) 254
BseBI CCWGG 3 cut(s) 372, 495, 769
BseDI CCNNGG 4 cut(s) 376, 655, 768, 803
BseGI GGATG 2 cut(s) 79, 759
BseJI GATNNNNATC 1 cut(s) 254
BseLI CCNNNNNNNGG 2 cut(s) 377, 769
BseMII CTCAG 2 cut(s) 353, 642
BseNI ACTGG 1 cut(s) 307
BseYI CCCAGC 1 cut(s) 707
Bsh1236I CGCG 1 cut(s) 720
BshFI GGCC 3 cut(s) 375, 493, 604
BsiSI CCGG 2 cut(s) 600, 619
BslI CCNNNNNNNGG 2 cut(s) 377, 769
BsnI GGCC 3 cut(s) 375, 493, 604
Bsp143I GATC 3 cut(s) 255, 342, 668
Bsp19I CCATGG 3 cut(s) 376, 655, 803
BspACI CCGC 1 cut(s) 720
BspANI GGCC 3 cut(s) 375, 493, 604
BspCNI CTCAG 2 cut(s) 352, 641
BspFNI CGCG 1 cut(s) 720
BspLI GGNNCC 1 cut(s) 550
BspPI GGATC 2 cut(s) 337, 676
BspTI CTTAAG 1 cut(s) 454
BsrI ACTGG 1 cut(s) 307
BssECI CCNNGG 4 cut(s) 376, 655, 768, 803
BssMI GATC 3 cut(s) 255, 342, 668
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 3 cut(s) 376, 655, 803
Bst2UI CCWGG 3 cut(s) 372, 495, 769
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 1 cut(s) 496
BstACI GRCGYC 1 cut(s) 9
BstAFI CTTAAG 1 cut(s) 454
BstBAI YACGTR 1 cut(s) 479
BstC8I GCNNGC 1 cut(s) 722
BstDEI CTNAG 2 cut(s) 339, 628
BstDSI CCRYGG 3 cut(s) 376, 655, 803
BstF5I GGATG 2 cut(s) 79, 759
BstFNI CGCG 1 cut(s) 720
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 4 cut(s) 158, 221, 707, 720
BstKTI GATC 3 cut(s) 258, 345, 671
BstMBI GATC 3 cut(s) 255, 342, 668
BstMWI GCNNNNNNNGC 1 cut(s) 819
BstNI CCWGG 3 cut(s) 372, 495, 769
BstSCI CCNGG 4 cut(s) 370, 493, 599, 767
BstSFI CTRYAG 1 cut(s) 294
BstSNI TACGTA 1 cut(s) 479
BstUI CGCG 1 cut(s) 720
BstV2I GAAGAC 3 cut(s) 81, 435, 783
BstX2I RGATCY 2 cut(s) 342, 668
BstXI CCANNNNNNTGG 1 cut(s) 353
BstYI RGATCY 2 cut(s) 342, 668
BsuRI GGCC 3 cut(s) 375, 493, 604
BtgI CCRYGG 3 cut(s) 376, 655, 803
BtrI CACGTC 1 cut(s) 433
BtsCI GGATG 2 cut(s) 79, 759
BtsIMutI CAGTG 1 cut(s) 429
Cac8I GCNNGC 1 cut(s) 722
CaiI CAGNNNCTG 1 cut(s) 66
CfoI GCGC 4 cut(s) 158, 221, 707, 720
Cfr13I GGNCC 3 cut(s) 368, 549, 602
Csp6I GTAC 3 cut(s) 632, 652, 808
CviAII CATG 5 cut(s) 57, 377, 489, 656, 804
CviQI GTAC 3 cut(s) 632, 652, 808
DdeI CTNAG 2 cut(s) 339, 628
DpnI GATC 3 cut(s) 257, 344, 670
DpnII GATC 3 cut(s) 255, 342, 668
DraI TTTAAA 1 cut(s) 27
DriI GACNNNNNGTC 1 cut(s) 85
EaeI YGGCCR 2 cut(s) 373, 491
Eam1104I CTCTTC 1 cut(s) 496
Eam1105I GACNNNNNGTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 496
Eco105I TACGTA 1 cut(s) 479
Eco130I CCWWGG 3 cut(s) 376, 655, 803
Eco47I GGWCC 2 cut(s) 368, 549
Eco47III AGCGCT 1 cut(s) 220
EcoRII CCWGG 3 cut(s) 370, 493, 767
EcoT14I CCWWGG 3 cut(s) 376, 655, 803
ErhI CCWWGG 3 cut(s) 376, 655, 803
FaeI CATG 5 cut(s) 60, 380, 492, 659, 807
FatI CATG 5 cut(s) 56, 376, 488, 655, 803
FauI CCCGC 1 cut(s) 713
FauNDI CATATG 1 cut(s) 526
FokI GGATG 2 cut(s) 86, 746
FspBI CTAG 1 cut(s) 513
GlaI GCGC 4 cut(s) 157, 220, 706, 719
GsaI CCCAGC 1 cut(s) 711
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 3 cut(s) 375, 493, 604
HapII CCGG 2 cut(s) 600, 619
HhaI GCGC 4 cut(s) 158, 221, 707, 720
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 5 cut(s) 60, 380, 492, 659, 807
Hin6I GCGC 4 cut(s) 156, 219, 705, 718
HinP1I GCGC 4 cut(s) 156, 219, 705, 718
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 3 cut(s) 86, 250, 534
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 2 cut(s) 600, 619
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 2 cut(s) 277, 368
Hpy188I TCNGA 5 cut(s) 128, 237, 255, 342, 539
Hpy188III TCNNGA 4 cut(s) 34, 101, 224, 484
Hpy8I GTNNAC 2 cut(s) 277, 368
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 774
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 3 cut(s) 432, 478, 650
HpyCH4V TGCA 7 cut(s) 4, 16, 60, 186, 315, 450, 793
HpyF10VI GCNNNNNNNGC 1 cut(s) 819
HpyF3I CTNAG 2 cut(s) 339, 628
HpySE526I ACGT 3 cut(s) 432, 478, 650
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 5 cut(s) 60, 380, 492, 659, 807
HspAI GCGC 4 cut(s) 156, 219, 705, 718
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 3 cut(s) 255, 342, 668
LmnI GCTCC 1 cut(s) 226
MaeI CTAG 1 cut(s) 513
MaeII ACGT 3 cut(s) 432, 478, 650
MaeIII GTNAC 2 cut(s) 139, 646
MalI GATC 3 cut(s) 257, 344, 670
MboI GATC 3 cut(s) 255, 342, 668
MboII GAAGA 6 cut(s) 81, 327, 440, 513, 663, 788
MflI RGATCY 2 cut(s) 342, 668
MlsI TGGCCA 2 cut(s) 375, 493
MluCI AATT 7 cut(s) 18, 50, 105, 173, 286, 445, 694
MluNI TGGCCA 2 cut(s) 375, 493
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 2 cut(s) 151, 562
MnlI CCTC 5 cut(s) 452, 497, 523, 676, 708
Mox20I TGGCCA 2 cut(s) 375, 493
MscI TGGCCA 2 cut(s) 375, 493
MseI TTAA 4 cut(s) 26, 150, 276, 455
Msp20I TGGCCA 2 cut(s) 375, 493
MspCI CTTAAG 1 cut(s) 454
MspI CCGG 2 cut(s) 600, 619
MspR9I CCNGG 4 cut(s) 372, 495, 601, 769
MvaI CCWGG 3 cut(s) 372, 495, 769
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 1 cut(s) 819
NciI CCSGG 1 cut(s) 601
NcoI CCATGG 3 cut(s) 376, 655, 803
NdeI CATATG 1 cut(s) 526
NdeII GATC 3 cut(s) 255, 342, 668
NlaIII CATG 5 cut(s) 60, 380, 492, 659, 807
NlaIV GGNNCC 1 cut(s) 550
NmuCI GTSAC 2 cut(s) 139, 646
PfeI GAWTC 2 cut(s) 250, 534
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
Ppu21I YACGTR 1 cut(s) 479
PshBI ATTAAT 1 cut(s) 150
Psp6I CCWGG 3 cut(s) 370, 493, 767
PspFI CCCAGC 1 cut(s) 707
PspGI CCWGG 3 cut(s) 370, 493, 767
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 3 cut(s) 368, 549, 602
PstNI CAGNNNCTG 1 cut(s) 66
PsuI RGATCY 2 cut(s) 342, 668
RsaI GTAC 3 cut(s) 633, 653, 809
RsaNI GTAC 3 cut(s) 632, 652, 808
SaqAI TTAA 4 cut(s) 26, 150, 276, 455
Sau3AI GATC 3 cut(s) 255, 342, 668
Sau96I GGNCC 3 cut(s) 368, 549, 602
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 4 cut(s) 372, 495, 601, 769
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 2 cut(s) 368, 549
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
SnaBI TACGTA 1 cut(s) 479
Sse9I AATT 7 cut(s) 18, 50, 105, 173, 286, 445, 694
SsiI CCGC 1 cut(s) 720
SspMI CTAG 1 cut(s) 513
StyD4I CCNGG 4 cut(s) 370, 493, 599, 767
StyI CCWWGG 3 cut(s) 376, 655, 803
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 3 cut(s) 435, 481, 653
TaqI TCGA 5 cut(s) 35, 92, 361, 585, 739
TasI AATT 7 cut(s) 18, 50, 105, 173, 286, 445, 694
TatI WGTACW 1 cut(s) 631
TfiI GAWTC 2 cut(s) 250, 534
Tru1I TTAA 4 cut(s) 26, 150, 276, 455
Tru9I TTAA 4 cut(s) 26, 150, 276, 455
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 2 cut(s) 139, 646
Tsp45I GTSAC 2 cut(s) 139, 646
TspDTI ATGAA 4 cut(s) 45, 243, 252, 748
TspRI CASTG 1 cut(s) 429
Vha464I CTTAAG 1 cut(s) 454
VpaK11BI GGWCC 2 cut(s) 368, 549
VspI ATTAAT 1 cut(s) 150
XapI RAATTY 3 cut(s) 50, 105, 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XspI CTAG 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.