RLG00000030024

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002703
Physical Location & Seq
Forward (+)
53469179 .. 53471553
2375 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030024

Sequence Viewer

Length: 1428 bp
ATGGAGGGTCGAATACTTGCTACGATGTTTTTGTTCATGATCATTATGGTTGCTTCACCTTCACCAAGTTCATGTGCCAGACTTAATGGTTTTCCCACAACATATACTTTAGGAGACGATGGTGCCATTGGTTATTACGGTGATCAAGTTGCTGTTGCGACATCAAATTCATTTCATGATAAGGAGTATGGTGATGTTTTTGCAACCCCAAATACATTCAAAGAGTATTATCGTCCTCTTGCAAATCGCCAAACCGATGATGATTTACAAGCATCCCCAAATGCATGGAGATCACTTGCAAAAAGCGAATTCAATGTGATGAATTATGGCGCTGTTGGGAATGGCCAAGTTGATGATACACAAGCCTTTCTAAAAGCATGGGGAGATGTTTGTGGATCCACTCAAGGCATCCCTACACTTATTATACCAAAGGGCAACACTTTCTTGCTCAATTCTGTGGAGTTTAAAGGGCCTTGCAGGGCTGCAAGTGTAAATTTTCAGCTAAGTGGGAACATTGTTGCACCAAATAATATGAATGCTTGGAAAGACAAGGAAAAATGGGTTCAGTTTGATAATGTTCAAGGGCTGATAATCAATGGAGGAGGTCAAATTGATGGTCAAGGTCAGGTTTGGTGGAAAGTTTGCGACACTACAAATTGCCAACGACCAACTGCTCTCTATATTTACAAGTGTGATGGTCTCCGCTTGAGTGATATTACACATATTAATAGTCCGAGAAACCATGTATTCGTCTTCGGCTGCAATGGCGTTCAAATTTCTGATATCCTTATTAGGGCTCCTGATGAAAGCCCGAACACTGATGGAATTAAAATCTCTAGATCAACCAATGTTAACATTGGCAATTCTCTTATAGGCACTGGTGACGACTGTATTGCAATCATTTCTGGTTCATCTCGTATCAACATTACTGATGTTATGTGTGGTCCAGGCCATGGTATAAGTGTTGGAAGTCTTGGTAAGCAAGGTTCTTATAGCACAGTGGAAGATGTGCAAGTGAGAAACTGCACCTTCAAGGGAACACAAAATGGAGCAAGAATCAAGACATGGCAAGGCGGGTCAGGGTATGCTAGGAACATCAGTTTTGAGGATATCATAATTGAAGCAGCCAAGAACCCTATCATTATAGACCAGAAGTATACTGATAATCTCTTTTCTGGAACTGGAACCCTGGTTGATCAAGAAAATGCTGTAAAAGTGAGCGATGTGACATTCCGTAACATTCGGGGATCTGTTGCTGGGGATACAGCCATTACTTTGGACTGTGACGATCGAATTGGTTGCAAAAACATTGTAATGGAAAACGTAGACTTAACTTCATCTGTTCCTGGCAAGAAGGTTTCTGTCAACTGCAAGAACGTTCAAGGATTATCTACTACATTATCTCCTAGTGTGCCATGCCTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

476

Amino Acids

51.22

Weight (kDa)

5.38

Isoelectric Point (pI)

25.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 135 - 461 9.7e-92 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 953
AccI GTMKAC 2 cut(s) 1157, 1326
AciI CCGC 2 cut(s) 703, 1074
AclI AACGTT 1 cut(s) 1377
AclWI GGATC 3 cut(s) 390, 403, 1255
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 4 cut(s) 166, 308, 493, 774
AfiI CCNNNNNNNGG 2 cut(s) 793, 953
AgsI TTSAA 7 cut(s) 220, 313, 581, 773, 1033, 1121, 1382
AjnI CCWGG 3 cut(s) 946, 1188, 1345
AluBI AGCT 1 cut(s) 502
AluI AGCT 1 cut(s) 502
Alw26I GTCTC 2 cut(s) 108, 704
AlwI GGATC 3 cut(s) 390, 403, 1255
AoxI GGCC 3 cut(s) 343, 470, 949
ApeKI GCWGC 3 cut(s) 482, 759, 1124
ApoI RAATTY 4 cut(s) 166, 308, 493, 774
ArsI GACNNNNNNTTYG 2 cut(s) 601, 633
AseI ATTAAT 1 cut(s) 726
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 470, 944
AsuHPI GGTGA 5 cut(s) 48, 54, 152, 203, 893
AvaII GGWCC 1 cut(s) 944
BalI TGGCCA 1 cut(s) 345
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 799
BbsI GAAGAC 1 cut(s) 745
BbvI GCAGC 3 cut(s) 469, 746, 1136
BccI CCATC 4 cut(s) 113, 608, 689, 815
BcgI CGANNNNNNTGC 4 cut(s) 875, 909, 1281, 1315
BciT130I CCWGG 3 cut(s) 948, 1190, 1347
BciVI GTATCC 1 cut(s) 1255
BclI TGATCA 3 cut(s) 39, 142, 1195
BcoDI GTCTC 2 cut(s) 108, 704
BfaI CTAG 3 cut(s) 837, 1089, 1407
BfoI RGCGCY 1 cut(s) 333
BfuI GTATCC 1 cut(s) 1255
BisI GCNGC 3 cut(s) 483, 760, 1125
BlsI GCNGC 3 cut(s) 484, 761, 1126
Bme1390I CCNGG 3 cut(s) 948, 1190, 1347
Bme18I GGWCC 1 cut(s) 944
BmgT120I GGNCC 2 cut(s) 470, 944
BmiI GGNNCC 4 cut(s) 124, 397, 798, 1186
BmrFI CCNGG 3 cut(s) 948, 1190, 1347
BmsI GCATC 2 cut(s) 281, 417
BpiI GAAGAC 1 cut(s) 745
BpuEI CTTGAG 2 cut(s) 387, 727
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 2 cut(s) 952, 1188
BsaXI ACNNNNNCTCC 2 cut(s) 1387, 1417
Bsc4I CCNNNNNNNGG 2 cut(s) 793, 953
Bse1I ACTGG 2 cut(s) 883, 1186
Bse3DI GCAATG 1 cut(s) 769
BseBI CCWGG 3 cut(s) 948, 1190, 1347
BseDI CCNNGG 2 cut(s) 952, 1188
BseGI GGATG 2 cut(s) 272, 408
BseLI CCNNNNNNNGG 2 cut(s) 793, 953
BseMI GCAATG 1 cut(s) 769
BseNI ACTGG 2 cut(s) 883, 1186
BseRI GAGGAG 1 cut(s) 615
BseXI GCAGC 3 cut(s) 469, 746, 1136
BseYI CCCAGC 1 cut(s) 1256
BsgI GTGCAG 1 cut(s) 1009
Bsh1285I CGRYCG 1 cut(s) 1291
BshFI GGCC 3 cut(s) 345, 472, 951
BshNI GGYRCC 1 cut(s) 122
BsiEI CGRYCG 1 cut(s) 1291
BslI CCNNNNNNNGG 2 cut(s) 793, 953
BsmAI GTCTC 2 cut(s) 108, 704
BsmBI CGTCTC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 3 cut(s) 345, 472, 951
Bso31I GGTCTC 1 cut(s) 704
Bsp1286I GDGCHC 1 cut(s) 799
Bsp143I GATC 8 cut(s) 39, 142, 290, 395, 839, 1195, 1247, 1288
Bsp19I CCATGG 1 cut(s) 952
BspACI CCGC 2 cut(s) 703, 1074
BspANI GGCC 3 cut(s) 345, 472, 951
BspHI TCATGA 2 cut(s) 36, 175
BspLI GGNNCC 4 cut(s) 124, 397, 798, 1186
BspPI GGATC 3 cut(s) 390, 403, 1255
BspT107I GGYRCC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 769
BsrI ACTGG 2 cut(s) 883, 1186
BssECI CCNNGG 2 cut(s) 952, 1188
BssMI GATC 8 cut(s) 39, 142, 290, 395, 839, 1195, 1247, 1288
BssNAI GTATAC 1 cut(s) 1158
BssT1I CCWWGG 1 cut(s) 952
Bst1107I GTATAC 1 cut(s) 1158
Bst2UI CCWGG 3 cut(s) 948, 1190, 1347
Bst4CI ACNGT 4 cut(s) 140, 890, 1000, 1283
BstDEI CTNAG 2 cut(s) 503, 1425
BstDSI CCRYGG 1 cut(s) 952
BstF5I GGATG 2 cut(s) 272, 408
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 8 cut(s) 42, 145, 293, 398, 842, 1198, 1250, 1291
BstMAI GTCTC 2 cut(s) 108, 704
BstMBI GATC 8 cut(s) 39, 142, 290, 395, 839, 1195, 1247, 1288
BstMCI CGRYCG 1 cut(s) 1291
BstMWI GCNNNNNNNGC 1 cut(s) 765
BstNI CCWGG 3 cut(s) 948, 1190, 1347
BstSCI CCNGG 3 cut(s) 946, 1188, 1345
BstV1I GCAGC 3 cut(s) 469, 746, 1136
BstV2I GAAGAC 1 cut(s) 745
BstX2I RGATCY 2 cut(s) 395, 1247
BstXI CCANNNNNNTGG 2 cut(s) 285, 1276
BstYI RGATCY 2 cut(s) 395, 1247
BstZ17I GTATAC 1 cut(s) 1158
BsuI GTATCC 1 cut(s) 1255
BsuRI GGCC 3 cut(s) 345, 472, 951
BtgI CCRYGG 1 cut(s) 952
BtgZI GCGATG 1 cut(s) 1236
BtsCI GGATG 2 cut(s) 272, 408
BtsIMutI CAGTG 3 cut(s) 816, 876, 1005
CciI TCATGA 2 cut(s) 36, 175
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 2 cut(s) 470, 944
CviAII CATG 9 cut(s) 37, 72, 176, 285, 378, 743, 953, 1065, 1416
DdeI CTNAG 2 cut(s) 503, 1425
DpnI GATC 8 cut(s) 41, 144, 292, 397, 841, 1197, 1249, 1290
DpnII GATC 8 cut(s) 39, 142, 290, 395, 839, 1195, 1247, 1288
DraI TTTAAA 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 952
Eco24I GRGCYC 1 cut(s) 799
Eco31I GGTCTC 1 cut(s) 704
Eco32I GATATC 2 cut(s) 784, 1111
Eco47I GGWCC 1 cut(s) 944
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 308
EcoRII CCWGG 3 cut(s) 946, 1188, 1345
EcoRV GATATC 2 cut(s) 784, 1111
EcoT14I CCWWGG 1 cut(s) 952
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 952
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 9 cut(s) 40, 75, 179, 288, 381, 746, 956, 1068, 1419
FatI CATG 9 cut(s) 36, 71, 175, 284, 377, 742, 952, 1064, 1415
FauI CCCGC 1 cut(s) 1067
FbaI TGATCA 3 cut(s) 39, 142, 1195
FblI GTMKAC 2 cut(s) 1157, 1326
Fnu4HI GCNGC 3 cut(s) 483, 760, 1125
FokI GGATG 2 cut(s) 259, 395
FriOI GRGCYC 1 cut(s) 799
Fsp4HI GCNGC 3 cut(s) 483, 760, 1125
FspBI CTAG 3 cut(s) 837, 1089, 1407
GlaI GCGC 1 cut(s) 331
GluI GCNGC 3 cut(s) 483, 760, 1125
GsaI CCCAGC 1 cut(s) 1260
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 3 cut(s) 345, 472, 951
HhaI GCGC 1 cut(s) 332
Hin1II CATG 9 cut(s) 40, 75, 179, 288, 381, 746, 956, 1068, 1419
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HincII GTYRAC 2 cut(s) 853, 1366
HindII GTYRAC 2 cut(s) 853, 1366
HinfI GANTC 1 cut(s) 1056
HpaI GTTAAC 1 cut(s) 853
HphI GGTGA 5 cut(s) 48, 54, 152, 203, 893
Hpy166II GTNNAC 4 cut(s) 853, 1158, 1327, 1366
Hpy188I TCNGA 2 cut(s) 735, 781
Hpy188III TCNNGA 7 cut(s) 37, 176, 800, 837, 1060, 1176, 1199
Hpy8I GTNNAC 4 cut(s) 853, 1158, 1327, 1366
HpyAV CCTTC 3 cut(s) 69, 1039, 1348
HpyCH4III ACNGT 4 cut(s) 140, 890, 1000, 1283
HpyCH4IV ACGT 2 cut(s) 1323, 1377
HpyF10VI GCNNNNNNNGC 1 cut(s) 765
HpyF3I CTNAG 2 cut(s) 503, 1425
HpySE526I ACGT 2 cut(s) 1323, 1377
Hsp92II CATG 9 cut(s) 40, 75, 179, 288, 381, 746, 956, 1068, 1419
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 3 cut(s) 39, 142, 1195
KspAI GTTAAC 1 cut(s) 853
Kzo9I GATC 8 cut(s) 39, 142, 290, 395, 839, 1195, 1247, 1288
LmnI GCTCC 2 cut(s) 802, 1049
Lsp1109I GCAGC 3 cut(s) 469, 746, 1136
LweI GCATC 2 cut(s) 281, 417
MaeI CTAG 3 cut(s) 837, 1089, 1407
MaeII ACGT 2 cut(s) 1323, 1377
MaeIII GTNAC 4 cut(s) 881, 1225, 1235, 1283
MalI GATC 8 cut(s) 41, 144, 292, 397, 841, 1197, 1249, 1290
MboI GATC 8 cut(s) 39, 142, 290, 395, 839, 1195, 1247, 1288
MboII GAAGA 2 cut(s) 745, 1016
MflI RGATCY 2 cut(s) 395, 1247
MhlI GDGCHC 1 cut(s) 799
MlsI TGGCCA 1 cut(s) 345
MluNI TGGCCA 1 cut(s) 345
MmeI TCCRAC 1 cut(s) 946
MnlI CCTC 4 cut(s) 246, 593, 596, 1099
Mox20I TGGCCA 1 cut(s) 345
Mph1103I ATGCAT 1 cut(s) 286
MscI TGGCCA 1 cut(s) 345
MseI TTAA 6 cut(s) 84, 465, 726, 828, 852, 1331
MslI CAYNNNNRTG 1 cut(s) 1313
Msp20I TGGCCA 1 cut(s) 345
MspR9I CCNGG 3 cut(s) 948, 1190, 1347
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 3 cut(s) 948, 1190, 1347
MwoI GCNNNNNNNGC 1 cut(s) 765
NcoI CCATGG 1 cut(s) 952
NdeII GATC 8 cut(s) 39, 142, 290, 395, 839, 1195, 1247, 1288
NlaIII CATG 9 cut(s) 40, 75, 179, 288, 381, 746, 956, 1068, 1419
NlaIV GGNNCC 4 cut(s) 124, 397, 798, 1186
NmuCI GTSAC 3 cut(s) 881, 1225, 1283
NsiI ATGCAT 1 cut(s) 286
PagI TCATGA 2 cut(s) 36, 175
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 1 cut(s) 1056
PflMI CCANNNNNTGG 1 cut(s) 953
PkrI GCNGC 3 cut(s) 484, 761, 1126
Ple19I CGATCG 1 cut(s) 1291
PshBI ATTAAT 1 cut(s) 726
Psp1406I AACGTT 1 cut(s) 1377
Psp6I CCWGG 3 cut(s) 946, 1188, 1345
PspFI CCCAGC 1 cut(s) 1256
PspGI CCWGG 3 cut(s) 946, 1188, 1345
PspN4I GGNNCC 4 cut(s) 124, 397, 798, 1186
PspPI GGNCC 2 cut(s) 470, 944
PsrI GAACNNNNNNTAC 2 cut(s) 970, 1002
PsuI RGATCY 2 cut(s) 395, 1247
PvuI CGATCG 1 cut(s) 1291
RseI CAYNNNNRTG 1 cut(s) 1313
SaqAI TTAA 6 cut(s) 84, 465, 726, 828, 852, 1331
SatI GCNGC 3 cut(s) 483, 760, 1125
Sau3AI GATC 8 cut(s) 39, 142, 290, 395, 839, 1195, 1247, 1288
Sau96I GGNCC 2 cut(s) 470, 944
ScrFI CCNGG 3 cut(s) 948, 1190, 1347
SduI GDGCHC 1 cut(s) 799
SfaNI GCATC 2 cut(s) 281, 417
SinI GGWCC 1 cut(s) 944
SmiMI CAYNNNNRTG 1 cut(s) 1313
SmlI CTYRAG 2 cut(s) 402, 706
SmoI CTYRAG 2 cut(s) 402, 706
SsiI CCGC 2 cut(s) 703, 1074
SspMI CTAG 3 cut(s) 837, 1089, 1407
StyD4I CCNGG 3 cut(s) 946, 1188, 1345
StyI CCWWGG 1 cut(s) 952
TaaI ACNGT 4 cut(s) 140, 890, 1000, 1283
TaiI ACGT 2 cut(s) 1326, 1380
TaqI TCGA 2 cut(s) 10, 1291
TfiI GAWTC 1 cut(s) 1056
Tru1I TTAA 6 cut(s) 84, 465, 726, 828, 852, 1331
Tru9I TTAA 6 cut(s) 84, 465, 726, 828, 852, 1331
TscAI CASTG 3 cut(s) 823, 883, 1005
TseFI GTSAC 3 cut(s) 881, 1225, 1283
TseI GCWGC 3 cut(s) 482, 759, 1124
Tsp45I GTSAC 3 cut(s) 881, 1225, 1283
TspDTI ATGAA 9 cut(s) 25, 60, 159, 164, 335, 548, 819, 900, 1326
TspGWI ACGGA 1 cut(s) 1223
TspRI CASTG 3 cut(s) 823, 883, 1005
Van91I CCANNNNNTGG 1 cut(s) 953
VpaK11BI GGWCC 1 cut(s) 944
VspI ATTAAT 1 cut(s) 726
XapI RAATTY 4 cut(s) 166, 308, 493, 774
XbaI TCTAGA 1 cut(s) 836
XmiI GTMKAC 2 cut(s) 1157, 1326
XspI CTAG 3 cut(s) 837, 1089, 1407
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.