Rmu_sc0017438.1_g000003

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017438.1
OG0002703
Physical Location & Seq
Forward (+)
3067 .. 5962
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017438.1_g000003.1.cds

Sequence Viewer

Length: 588 bp
atgaccagacgcaacaaactcagcgactcgatgaagtcgaagtccaccgccgtccccggaagagcgatcagagagaagagaaaatcccacctttccgattgtaggtttaccgccggcactcgagttagactgccggcactcaagagtgcaaagccagatacctatctttggccttctgaggttgagacaacgagagcaacgccggataacacccccaccagtaatgaagggagtaatttagattctttgaatctcaaacaatgcaaagcctatttgcgaacggccaagaaccctatcattatagaccagaagtatactgataatctctctactggaactggaactggaaccctgcttgaccaagaaaatgctgtacaagtgagcgatgtgacattccgtaacattcggggatctgttgctggggatacagccattactttggactgtgaccatcaaattggttgcaaaaacattgtaatggatcacatagacttaacttcatctgttcctggcaagaaggtttctgcccactgcgagaacgttaaaggattatctactttattatctcccagtgtgtcatgtctttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

195

Amino Acids

21.18

Weight (kDa)

9.18

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 314
AciI CCGC 2 cut(s) 48, 111
AclI AACGTT 1 cut(s) 540
AclWI GGATC 2 cut(s) 418, 489
AcoI YGGCCR 1 cut(s) 282
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 2 cut(s) 102, 168
AgsI TTSAA 1 cut(s) 250
AjnI CCWGG 1 cut(s) 508
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 2 cut(s) 418, 489
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 2 cut(s) 170, 282
AsuC2I CCSGG 1 cut(s) 57
AvaI CYCGRG 1 cut(s) 120
BccI CCATC 1 cut(s) 459
BceAI ACGGC 2 cut(s) 35, 297
BciT130I CCWGG 1 cut(s) 510
BciVI GTATCC 1 cut(s) 418
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 179
BfuI GTATCC 1 cut(s) 418
Bme1390I CCNGG 2 cut(s) 57, 510
BmeT110I CYCGRG 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 2 cut(s) 57, 510
BmrI ACTGGG 1 cut(s) 564
BmuI ACTGGG 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 55
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 168
Bse118I RCCGGY 2 cut(s) 113, 133
Bse1I ACTGG 5 cut(s) 219, 337, 343, 349, 570
Bse8I GATNNNNATC 1 cut(s) 162
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 1 cut(s) 55
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 2 cut(s) 102, 168
BseMII CTCAG 2 cut(s) 34, 168
BseNI ACTGG 5 cut(s) 219, 337, 343, 349, 570
BseYI CCCAGC 1 cut(s) 419
BshFI GGCC 2 cut(s) 172, 284
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 4 cut(s) 57, 114, 134, 203
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 2 cut(s) 102, 168
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 2 cut(s) 172, 284
BsoBI CYCGRG 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 3 cut(s) 66, 410, 481
BspACI CCGC 2 cut(s) 48, 111
BspANI GGCC 2 cut(s) 172, 284
BspCNI CTCAG 2 cut(s) 33, 169
BspLI GGNNCC 1 cut(s) 349
BspPI GGATC 2 cut(s) 418, 489
BspQI GCTCTTC 1 cut(s) 55
BsrFI RCCGGY 2 cut(s) 113, 133
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 5 cut(s) 219, 337, 343, 349, 570
BssAI RCCGGY 2 cut(s) 113, 133
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 3 cut(s) 66, 410, 481
BssNAI GTATAC 1 cut(s) 315
Bst1107I GTATAC 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 510
Bst4CI ACNGT 1 cut(s) 446
Bst6I CTCTTC 2 cut(s) 55, 71
BstAUI TGTACA 1 cut(s) 373
BstC8I GCNNGC 2 cut(s) 115, 135
BstDEI CTNAG 2 cut(s) 20, 177
BstKTI GATC 3 cut(s) 69, 413, 484
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 3 cut(s) 66, 410, 481
BstNI CCWGG 1 cut(s) 510
BstSCI CCNGG 2 cut(s) 55, 508
BstX2I RGATCY 1 cut(s) 410
BstXI CCANNNNNNTGG 2 cut(s) 439, 458
BstYI RGATCY 1 cut(s) 410
BstZ17I GTATAC 1 cut(s) 315
BsuI GTATCC 1 cut(s) 418
BsuRI GGCC 2 cut(s) 172, 284
BtgZI GCGATG 1 cut(s) 399
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 2 cut(s) 529, 577
Cac8I GCNNGC 2 cut(s) 115, 135
Cfr10I RCCGGY 2 cut(s) 113, 133
CseI GACGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 374
CviAII CATG 1 cut(s) 579
CviJI RGCY 5 cut(s) 154, 172, 269, 284, 431
CviKI_1 RGCY 5 cut(s) 154, 172, 269, 284, 431
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 2 cut(s) 20, 177
DpnI GATC 3 cut(s) 68, 412, 483
DpnII GATC 3 cut(s) 66, 410, 481
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 2 cut(s) 55, 71
EarI CTCTTC 2 cut(s) 55, 71
Eco88I CYCGRG 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 508
FaeI CATG 1 cut(s) 582
FaiI YATR 4 cut(s) 302, 315, 488, 580
FaqI GGGAC 1 cut(s) 38
FatI CATG 1 cut(s) 578
FblI GTMKAC 1 cut(s) 314
GsaI CCCAGC 1 cut(s) 423
HaeIII GGCC 2 cut(s) 172, 284
HapII CCGG 4 cut(s) 57, 114, 134, 203
HgaI GACGC 1 cut(s) 18
Hin1II CATG 1 cut(s) 582
HinfI GANTC 3 cut(s) 26, 242, 250
HpaII CCGG 4 cut(s) 57, 114, 134, 203
Hpy166II GTNNAC 3 cut(s) 45, 108, 315
Hpy188I TCNGA 3 cut(s) 71, 97, 178
Hpy188III TCNNGA 1 cut(s) 142
Hpy8I GTNNAC 3 cut(s) 45, 108, 315
HpyAV CCTTC 3 cut(s) 183, 221, 511
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 3 cut(s) 149, 264, 465
HpyF3I CTNAG 2 cut(s) 20, 177
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 1 cut(s) 582
KroI GCCGGC 2 cut(s) 113, 133
KroNI GCCGGC 2 cut(s) 115, 135
Kzo9I GATC 3 cut(s) 66, 410, 481
LguI GCTCTTC 1 cut(s) 55
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 3 cut(s) 388, 398, 446
MalI GATC 3 cut(s) 68, 412, 483
MboI GATC 3 cut(s) 66, 410, 481
MboII GAAGA 2 cut(s) 72, 88
MflI RGATCY 1 cut(s) 410
MluCI AATT 2 cut(s) 235, 456
MlyI GAGTC 1 cut(s) 20
MnlI CCTC 1 cut(s) 172
MroNI GCCGGC 2 cut(s) 113, 133
MseI TTAA 3 cut(s) 494, 543, 586
MslI CAYNNNNRTG 1 cut(s) 476
MspI CCGG 4 cut(s) 57, 114, 134, 203
MspR9I CCNGG 2 cut(s) 57, 510
MvaI CCWGG 1 cut(s) 510
NaeI GCCGGC 2 cut(s) 115, 135
NciI CCSGG 1 cut(s) 57
NdeII GATC 3 cut(s) 66, 410, 481
NgoMIV GCCGGC 2 cut(s) 113, 133
NlaIII CATG 1 cut(s) 582
NlaIV GGNNCC 1 cut(s) 349
NmuCI GTSAC 2 cut(s) 388, 446
PaeR7I CTCGAG 1 cut(s) 120
PciSI GCTCTTC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PdiI GCCGGC 2 cut(s) 115, 135
PfeI GAWTC 2 cut(s) 242, 250
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
Psp1406I AACGTT 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 508
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 1 cut(s) 349
PspXI VCTCGAGB 1 cut(s) 120
PsuI RGATCY 1 cut(s) 410
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
RseI CAYNNNNRTG 1 cut(s) 476
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 3 cut(s) 494, 543, 586
Sau3AI GATC 3 cut(s) 66, 410, 481
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 57, 510
SetI ASST 6 cut(s) 93, 107, 164, 183, 522, 543
Sfr274I CTCGAG 1 cut(s) 120
SlaI CTCGAG 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 2 cut(s) 120, 140
SmoI CTYRAG 2 cut(s) 120, 140
Sse9I AATT 2 cut(s) 235, 456
SsiI CCGC 2 cut(s) 48, 111
StyD4I CCNGG 2 cut(s) 55, 508
TaaI ACNGT 1 cut(s) 446
TaiI ACGT 1 cut(s) 543
TaqI TCGA 3 cut(s) 29, 38, 121
TasI AATT 2 cut(s) 235, 456
TatI WGTACW 1 cut(s) 373
TfiI GAWTC 2 cut(s) 242, 250
Tru1I TTAA 3 cut(s) 494, 543, 586
Tru9I TTAA 3 cut(s) 494, 543, 586
TscAI CASTG 2 cut(s) 536, 577
TseFI GTSAC 2 cut(s) 388, 446
Tsp45I GTSAC 2 cut(s) 388, 446
TspDTI ATGAA 3 cut(s) 47, 240, 489
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 2 cut(s) 536, 577
XhoI CTCGAG 1 cut(s) 120
XmiI GTMKAC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.