Rorug04G0233600

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002703
Physical Location & Seq
Forward (+)
39860458 .. 39860988
531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0233600.1

Sequence Viewer

Length: 531 bp
ATGGCCGGTAAGAGGAGGGAAGAGGTAGAACGTTGGACCACAGAACTAACTCCCAAAATGGAGGAAAGATTGAAGGTGCAAATGGAGAAATCTCGTCGTTTCAGTGTCCATTATTCTAGCCCTGGAGTTTATGAGGTTCGATCTGACTTTTCATATGTAGTCAACATCTCCGATCATTCATGTTCACGTGTGAAATGGCAGATCAATTTTTTTCCTTGTCCTCACGGCCTTGCTGCATTACAGGCTGCTTCTGTAAATGTATATGATTATATTGATAAGTACTTTCAGGTTGATATGTTCAAGAAGAGCTACAGTTTTCCTATCTGTCCGATAACCAATGTTGATATGTCTTCTTCAGAATCTGCTTCTGAATGTATATTACCTCCCCTTTCGAAGAGGCCCCCCGGGAGGCCTAGGGTGAAGCGGTTCAAGTCGGTTGGAGAGGCTGAAAAGAAGCTGATTCGTTGTGGTCGTTGTGGCAAAATGGGCACTCATAACAAGTTGAGCTGCACTGAACCTCTCGTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

176

Amino Acids

20.22

Weight (kDa)

9.36

Isoelectric Point (pI)

68.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 424
AclI AACGTT 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 339
AcvI CACGTG 1 cut(s) 188
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 2 cut(s) 12, 408
AflIII ACRYGT 1 cut(s) 187
AgsI TTSAA 3 cut(s) 73, 301, 430
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 3 cut(s) 309, 457, 507
AluI AGCT 3 cut(s) 309, 457, 507
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 4 cut(s) 3, 226, 398, 410
ApeKI GCWGC 3 cut(s) 233, 245, 507
Asp700I GAANNNNTTC 1 cut(s) 425
AspA2I CCTAGG 1 cut(s) 413
AspS9I GGNCC 2 cut(s) 36, 399
AsuC2I CCSGG 2 cut(s) 405, 406
AsuHPI GGTGA 1 cut(s) 430
AsuII TTCGAA 1 cut(s) 392
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 36
AvrII CCTAGG 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 491
BbrPI CACGTG 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 342
BbvI GCAGC 3 cut(s) 220, 232, 494
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 1 cut(s) 123
BcnI CCSGG 2 cut(s) 405, 406
BfaI CTAG 2 cut(s) 117, 414
BfmI CTRYAG 1 cut(s) 310
BisI GCNGC 3 cut(s) 234, 246, 508
BlnI CCTAGG 1 cut(s) 413
BlsI GCNGC 3 cut(s) 235, 247, 509
BmcAI AGTACT 1 cut(s) 281
Bme1390I CCNGG 3 cut(s) 123, 405, 406
Bme18I GGWCC 1 cut(s) 36
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 2 cut(s) 36, 399
BmiI GGNNCC 1 cut(s) 401
BmrFI CCNGG 3 cut(s) 123, 405, 406
BpiI GAAGAC 1 cut(s) 342
BpmI CTGGAG 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 392
BpuMI CCSGG 2 cut(s) 405, 406
BsaAI YACGTR 1 cut(s) 188
BsaJI CCNNGG 4 cut(s) 121, 403, 404, 413
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 408
Bse118I RCCGGY 1 cut(s) 5
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 4 cut(s) 121, 403, 404, 413
BseLI CCNNNNNNNGG 2 cut(s) 12, 408
BseRI GAGGAG 1 cut(s) 28
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 3 cut(s) 220, 232, 494
BsgI GTGCAG 1 cut(s) 493
BshFI GGCC 4 cut(s) 5, 228, 400, 412
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 2 cut(s) 6, 405
BslI CCNNNNNNNGG 2 cut(s) 12, 408
BsnI GGCC 4 cut(s) 5, 228, 400, 412
BsoBI CYCGRG 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 392
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 3 cut(s) 140, 172, 201
BspACI CCGC 1 cut(s) 424
BspANI GGCC 4 cut(s) 5, 228, 400, 412
BspLI GGNNCC 1 cut(s) 401
BspQI GCTCTTC 1 cut(s) 299
BspT104I TTCGAA 1 cut(s) 392
BsrFI RCCGGY 1 cut(s) 5
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 4 cut(s) 121, 403, 404, 413
BssMI GATC 3 cut(s) 140, 172, 201
BssT1I CCWWGG 1 cut(s) 413
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 3 cut(s) 15, 299, 389
BstBAI YACGTR 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 392
BstKTI GATC 3 cut(s) 143, 175, 204
BstMBI GATC 3 cut(s) 140, 172, 201
BstMWI GCNNNNNNNGC 2 cut(s) 242, 486
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 3 cut(s) 121, 403, 404
BstSFI CTRYAG 1 cut(s) 310
BstSLI GKGCMC 1 cut(s) 491
BstV1I GCAGC 3 cut(s) 220, 232, 494
BstV2I GAAGAC 1 cut(s) 342
BsuRI GGCC 4 cut(s) 5, 228, 400, 412
BtsIMutI CAGTG 2 cut(s) 109, 510
CaiI CAGNNNCTG 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 2 cut(s) 36, 399
Cfr9I CCCGGG 1 cut(s) 404
Csp6I GTAC 1 cut(s) 280
CviAII CATG 1 cut(s) 180
CviQI GTAC 1 cut(s) 280
DpnI GATC 3 cut(s) 142, 174, 203
DpnII GATC 3 cut(s) 140, 172, 201
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 15, 299, 389
EarI CTCTTC 3 cut(s) 15, 299, 389
Eco130I CCWWGG 1 cut(s) 413
Eco147I AGGCCT 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 339
Eco72I CACGTG 1 cut(s) 188
Eco88I CYCGRG 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 1 cut(s) 183
FatI CATG 1 cut(s) 179
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 3 cut(s) 234, 246, 508
Fsp4HI GCNGC 3 cut(s) 234, 246, 508
FspBI CTAG 2 cut(s) 117, 414
GluI GCNGC 3 cut(s) 234, 246, 508
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 4 cut(s) 5, 228, 400, 412
HapII CCGG 2 cut(s) 6, 405
Hin1II CATG 1 cut(s) 183
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 2 cut(s) 359, 460
HpaII CCGG 2 cut(s) 6, 405
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 2 cut(s) 163, 185
Hpy188I TCNGA 5 cut(s) 145, 172, 330, 358, 370
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 2 cut(s) 163, 185
Hpy99I CGWCG 1 cut(s) 99
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4IV ACGT 2 cut(s) 31, 187
HpyCH4V TGCA 3 cut(s) 79, 236, 510
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 486
HpySE526I ACGT 2 cut(s) 31, 187
Hsp92II CATG 1 cut(s) 183
Kzo9I GATC 3 cut(s) 140, 172, 201
LguI GCTCTTC 1 cut(s) 299
LpnPI CCDG 6 cut(s) 19, 108, 135, 227, 272, 418
Lsp1109I GCAGC 3 cut(s) 220, 232, 494
MaeI CTAG 2 cut(s) 117, 414
MaeII ACGT 2 cut(s) 31, 187
MalI GATC 3 cut(s) 142, 174, 203
MboI GATC 3 cut(s) 140, 172, 201
MboII GAAGA 5 cut(s) 32, 316, 342, 345, 406
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 1 cut(s) 205
MmeI TCCRAC 2 cut(s) 14, 418
MroXI GAANNNNTTC 1 cut(s) 425
MspI CCGG 2 cut(s) 6, 405
MspR9I CCNGG 3 cut(s) 123, 405, 406
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 242, 486
NciI CCSGG 2 cut(s) 405, 406
NdeI CATATG 1 cut(s) 154
NdeII GATC 3 cut(s) 140, 172, 201
NlaIII CATG 1 cut(s) 183
NlaIV GGNNCC 1 cut(s) 401
NspV TTCGAA 1 cut(s) 392
PceI AGGCCT 1 cut(s) 412
PciSI GCTCTTC 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 469
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 2 cut(s) 359, 460
PkrI GCNGC 3 cut(s) 235, 247, 509
PmaCI CACGTG 1 cut(s) 188
PmlI CACGTG 1 cut(s) 188
Ppu21I YACGTR 1 cut(s) 188
Psp1406I AACGTT 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 121
PspCI CACGTG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 401
PspPI GGNCC 2 cut(s) 36, 399
PstNI CAGNNNCTG 1 cut(s) 362
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SapI GCTCTTC 1 cut(s) 299
SatI GCNGC 3 cut(s) 234, 246, 508
Sau3AI GATC 3 cut(s) 140, 172, 201
Sau96I GGNCC 2 cut(s) 36, 399
ScaI AGTACT 1 cut(s) 281
ScrFI CCNGG 3 cut(s) 123, 405, 406
SduI GDGCHC 1 cut(s) 491
SfcI CTRYAG 1 cut(s) 310
SfuI TTCGAA 1 cut(s) 392
SinI GGWCC 1 cut(s) 36
SmaI CCCGGG 1 cut(s) 406
Sse9I AATT 1 cut(s) 205
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 1 cut(s) 424
SspMI CTAG 2 cut(s) 117, 414
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 3 cut(s) 121, 403, 404
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 1 cut(s) 314
TaiI ACGT 2 cut(s) 34, 190
TaqI TCGA 2 cut(s) 139, 392
TasI AATT 1 cut(s) 205
TatI WGTACW 1 cut(s) 279
TfiI GAWTC 2 cut(s) 359, 460
TscAI CASTG 2 cut(s) 109, 517
TseI GCWGC 3 cut(s) 233, 245, 507
TspDTI ATGAA 2 cut(s) 141, 168
TspMI CCCGGG 1 cut(s) 404
TspRI CASTG 2 cut(s) 109, 517
VpaK11BI GGWCC 1 cut(s) 36
XmaI CCCGGG 1 cut(s) 404
XmaJI CCTAGG 1 cut(s) 413
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 2 cut(s) 117, 414
ZrmI AGTACT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.