FvH4_1g05520

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001838
Physical Location & Seq
Reverse (-)
2904576 .. 2911695
7120 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05520.t8

Sequence Viewer

Length: 4014 bp
ATGACCACAACCATGACCACTAATACTAGTTCGTCGCAGATTCTCATCCACACGGCCGCGTTCGTCTCCGAAACCCTTTCGCAATCGGACCTCCGCAGCCACCTCCTCTCCACGCTCCGCCGCAGGCTGCCGCCGTCGCAGTACTCCAACCAAGCAAAGCTCAAGCCCCTCAACCTCGCCGCCGAAACCCTAGAAAACGCCATCGCCGCCGAGAGCGCCGCAGTCCGGACCTCCTCCCTCCGCCTCTGCGAGAAGCTCCTCCTCTCCGGGCCGAAAAGCCCCTTCGCCTCTCTCCTCCTCTCCCTCATCTACGGCCTCTGCCGCCGCTCGATCGACGCCGCCATCTGCATCCTCGAGATCTTCAAAACCGAGCCGGCGCTCGCCCGGACGGAGGTGGCGCCGGTGCTGTTCGAGGAGCTCTTCCTCGTCCACCTCCTCCCGGTGCTGCAATGGTACAACCGCCGCCGGTCGCAGATCCTGCCGGCGATGTCCCCAAACGTGAGCTACGAAAACGACGACGTTTCGATCAACGACATGTCGGCGGCGTTTTTCCCGGGGTCGAATTCGCTGTCGAAGATGAGCGGGGTTCAGACGGTGGGGCTGAAGGAGCTGGAGAGCAGCTACGAGAGGGTTTTGGATGAGAATTGTAGGGTTTTTAGTGAGTATTTCAGGGAGGTTTTGGAGAATAAAGACGGTGGGAGAGTGATTGAGCCGCCGGCTGCGGTGTTGGAATTGAGTAGTGATGAATTCCGGTATGATGATCGGAGTAGTAGTACCGGTGAAGATGACGCCGGATTTGGAAACGGACGGTACAATCCAATATGGGCTGAAGCAGAAAATTCAGTTGAATCGTTCAGCACGGGCAGCAGTGTAAGAAGACCCAGGTCTCCCCCGTTGTATCCTCAAAGAGTTGCACTTGATAATATCATCACTGGCCACAAACCTTCATGGATGTTGGGAAGAAGGCCACGTGTAAATTCCGATTCTGAGCTGGAAGAGTCTTCATTAGAGGAGCATTCTCTGGATTCTTCTTTGGATTCCGAAGCAGAAATTGAGGAAAACAACACAAGAATGGAATTGTTTGATTCTGGACAGAATCAAATCCAAAAGCTAAAGCAACCCATTTCCGCAGAATCAAGTCGTTCATCTCCAAACCGGCTCATGGCAGATTCTGAAAACCAATCTAGTAGTGGAAAGTTTACACCTCCTAAGGACTTTGTGTGTCCCATAACTAGTACCCTTTTTGATGATCCAGTGACACTTGAGACTGGTCAGACATACGAGCGAAAAGCCATCCAAGAATGGCTTGATAGAGGAAATTCAACCTGCCCAATTACACGCCAGAACTTGCAAAGTACCCAACTGCCTAAGACCAACTATGTCCTCAAACGTCTGATTGCAAGTTGGCAAGAGCAGAACCCTGGTATAGTCTTCAGCCCATCCGATAATCCAAGCCCGCAGATTGAGCCAATGGTGAAATCCATAATGCCATCAACTTCTCCTGTCAGTGTCATAAGCCAAGCTGCAATTGATAGTGCAGTTAGTGAGTTGAGAATTGCCATCACCAATCTTTGTATGTCAGAAATTCTCAAGGAGTCTGAAATGGCTGTTCTTCGTATTGAGCGCTTCTGGCTGGAAGCAAATGTGGAATGGGACATTCAGAATATGCTCTCAAAACCTCCAGTAATTAATGGTTTTGTGGAGGTTCTTTTCAATTCTGTTGATCCTAGAGTTCTAACAGCTACGGTTTTCCTCTTGTCTGAGCTGGGTTCAAGAGACAATGCTGTGATTCAGACACTAACCCAGGTTGACTCTGATGTGGAATGTATTGTGGCTCTTTTCAAAAAGGGGTTGATGGAGGCTGTTGTCTTGATATATCTGTTAAGGCATTGCATTCTTAATCTAGTAGAGATGGACCTGGTGGACTCTCTCTTATCCGTTATTAGAAAGAGAAATGATGATTTCCCTAATATGTATCTAAAGCCAAAATCAGCTGCAGTGGTTTTACTGGGGCATATTGTTGGTGTCAGTGAATCGATTGCCAGCTCTTTGACTTCTGAAAAATCACTTGAAAGAATAATCAGCAGTTTGGAATCTGAATCAGCAGAAGAGAAGACTGCAGCGATTGGTATCTTATTAAGGTGTATGCAACAAGATGGGCACTGCAGGAACACAATAGCTGATAAAGCTGAGTTAGCTCCTGTTTTGGATAGCTTCACGGGTGCAAAGGATGAAGAACGGTTTGAGATAGTTCTCTTTTTGTCTGAGTTAGTAAAATTAAACAGGAGGACATTCAATGAACAAATCCTGCATATCATAAAGGATGAGGGCCCTTTCAGTACAATGCACACCCTCCTTATTTATCTTCAGACTGCTGCTCAAGACCATTGTCCTGTTGTGGCTGGTCTACTACTTCAACTTGATCTTCTGGCAGAGCCAAGAAAGATGAGCATATTCCGTGAAGAAGCAATGGACACTCTCATCTCATGTCTCAGAAATACTGAGTTCCCATCTGCCCAAATCGCTGCTGCTGAAACCATTATGTCACTGCAAGGGAGGTTTACCACATCCGGAAAGCCTCTTGCTAGGGCCTTCCTTCTAAAACGTGCTGGCATTGACAAAATTTATAAACAACGCGTGCGAATGGATCAACTTAGCAGCTTTTCTGGAGAAGATGAAACTCTAGATGAAGAAAAGGCGGCTGATAACTGGGAAAGAAAATTGGCATTTGTTCTTGCAAGCCACGAGTTTGGTCTACTTTTTGAGGCATTGGCAGAAGGATTGAAGAGCAGAATCTCAGAATTATGTTCTGCATGCTTTGTGTCAGCCACATGGCTCGTACATATGCTCGATGTCCTTCCAGATACAGGGATACGGGGAGCAGCTCGGCTGTGCTTGATTAAACGCTTCATATCAATATTTAAGTCTGCCAAGGATACTGAAGACAAAGCTCTTGCTTTGCTGGCATTGGGCAGCTTCATCCATGATCCCGAGGGTATGCAAGAATTAAATTCTTGCAGGAGGGATATTGTAAAAGGCCTGAGAGAGCTAAAGAGATACACTCCCTTGGCGTTTCAAATGCTAAAAGTGTTTTCAGAAGGGCAAGACTCTAGTGCAGAGCTGTGGGATCATAAAGAATTAGTTCAAGTAGATTGCAGTGAGAACGGAGAGGTTCTTTCAGTAGTTTGCTTCAAGGATAAAATATTTTCTGGCCATTCAGATGGAACTATAAAGGTCTGGACGGGTAAAGGTAGCATTCTCCATCTAATCCAAGAAACTCAAGAACATACAAAGGCAGTGTCTAGCTTGGCAATTTTGCAATCAAAGGAGATTCTATACAGTGGTTCACTAGATAAAACAACAAGGGCCTGGTCTATAAGCAATGAAGGAATCCAATGTGTAGAAGTTCATGATATGAAGGATCAAGTTCATAATCTGGCCGTCACCAATAGCTTTTCATGCTTCATTCCACAAAGCAATGGTATCAAGGCTCACTCATGGAATGGAGGATCCAAAATGTTAAATTCGGGCAAATCTGTAAAGTGCTTTGCTCTGGTAAATGGGAAAATATTTTGTGGATGCCATGATAGCACTGTTCAGGAGATTGATTTGGTAACAGGAACACTCAGTACCATTCAGACCGGCTCCCGGAAATTACTTGGGAAATCAAATCCTATCCATTGCATTCAGTATCATAGTGGATTGATATACGCAGCAGGTTCTTCGTTGGATGGATCAGCAGTGAAGATTTGGAGTGCTGCAAACTTCAGTTTGGTTGGGTCTCTGTCAACTACACTAGAAGTGCGGGCAATGACATTAAGCTCGGAACTGATCTATTTGGGTGGTAAAGGGGGATGTGTGGAAATTTGGGATAGGGAGAAGCAGAACAAAATAATAGACACACTACAAACTGGCACAAACTGTAAGGTTCTTTCCTTGGCTCTTGATGCCAATGAGGAAGTTTTGGTTACTGGAACTTCTGATGGCCGAATCCAGGCATGGGGATTGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1338

Amino Acids

147.89

Weight (kDa)

5.33

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 93 - 230 1.4e-17 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 403 - 473 3.6e-17 U-box domain
ARM_LIN_2nd PF23654 513 - 670 5.3e-59 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 679 - 998 5.5e-133 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2628
Acc36I ACCTGC 2 cut(s) 1334, 3707
AccB1I GGYRCC 1 cut(s) 397
AccBSI CCGCTC 2 cut(s) 327, 582
AccI GTMKAC 2 cut(s) 2407, 2755
AccII CGCG 2 cut(s) 59, 2637
AccIII TCCGGA 2 cut(s) 225, 2570
AcoI YGGCCR 5 cut(s) 54, 934, 3211, 3438, 3985
AcuI CTGAAG 6 cut(s) 623, 849, 1417, 2351, 2961, 3751
AcvI CACGTG 1 cut(s) 971
AcyI GRCGYC 3 cut(s) 336, 398, 789
AfaI GTAC 9 cut(s) 143, 455, 775, 812, 1237, 1357, 2341, 2841, 3631
AfeI AGCGCT 1 cut(s) 1625
AfiI CCNNNNNNNGG 8 cut(s) 225, 391, 439, 1162, 2867, 3648, 3994, 4000
AflIII ACRYGT 3 cut(s) 534, 970, 2635
AgeI ACCGGT 1 cut(s) 776
AhlI ACTAGT 2 cut(s) 26, 1232
AjnI CCWGG 6 cut(s) 881, 1420, 1803, 1917, 3368, 3993
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 6 cut(s) 70, 891, 1259, 1770, 2495, 3786
AlwNI CAGNNNCTG 2 cut(s) 478, 1172
Ama87I CYCGRG 3 cut(s) 353, 553, 2990
Aor13HI TCCGGA 2 cut(s) 225, 2570
Aor51HI AGCGCT 1 cut(s) 1625
ApaI GGGCCC 1 cut(s) 2333
ArsI GACNNNNNNTTYG 6 cut(s) 554, 586, 779, 811, 3284, 3316
AseI ATTAAT 1 cut(s) 1689
AsiGI ACCGGT 1 cut(s) 776
Asp700I GAANNNNTTC 1 cut(s) 3141
AspLEI GCGC 4 cut(s) 218, 379, 400, 1626
AspS9I GGNCC 8 cut(s) 88, 228, 269, 1915, 2329, 2330, 2589, 3366
AsuC2I CCSGG 6 cut(s) 268, 385, 440, 554, 555, 3649
AsuHPI GGTGA 4 cut(s) 791, 1486, 1555, 3436
AvaI CYCGRG 3 cut(s) 353, 553, 2990
AvaII GGWCC 3 cut(s) 88, 228, 1915
AxyI CCTNAGG 1 cut(s) 1209
BaeGI GKGCMC 2 cut(s) 2163, 2333
BalI TGGCCA 2 cut(s) 936, 3213
BamHI GGATCC 1 cut(s) 3509
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 2 cut(s) 420, 2333
BauI CACGAG 1 cut(s) 2744
BbrPI CACGTG 1 cut(s) 971
BbsI GAAGAC 5 cut(s) 883, 993, 1423, 2120, 2949
Bbv12I GWGCWC 1 cut(s) 420
BceAI ACGGC 4 cut(s) 69, 118, 328, 3425
BcgI CGANNNNNNTGC 2 cut(s) 3705, 3739
BciT130I CCWGG 6 cut(s) 883, 1422, 1805, 1919, 3370, 3995
BciVI GTATCC 3 cut(s) 909, 2865, 2929
BcnI CCSGG 6 cut(s) 268, 385, 440, 554, 555, 3649
BcoDI GTCTC 6 cut(s) 70, 891, 1259, 1770, 2495, 3786
BcuI ACTAGT 2 cut(s) 26, 1232
BfmI CTRYAG 3 cut(s) 1995, 2118, 2164
BfoI RGCGCY 4 cut(s) 219, 380, 401, 1627
BfuAI ACCTGC 2 cut(s) 1334, 3707
BfuI GTATCC 3 cut(s) 909, 2865, 2929
BglII AGATCT 1 cut(s) 357
BmcAI AGTACT 1 cut(s) 143
Bme18I GGWCC 3 cut(s) 88, 228, 1915
BmeT110I CYCGRG 3 cut(s) 353, 553, 2990
BmgT120I GGNCC 8 cut(s) 88, 228, 269, 1915, 2329, 2330, 2589, 3366
BmiI GGNNCC 4 cut(s) 399, 2331, 3511, 3646
BmrI ACTGGG 2 cut(s) 2018, 2719
BmsI GCATC 3 cut(s) 357, 3569, 3937
BmuI ACTGGG 2 cut(s) 2018, 2719
BoxI GACNNNNGTC 2 cut(s) 883, 2388
BpiI GAAGAC 5 cut(s) 883, 993, 1423, 2120, 2949
BplI GAGNNNNNCTC 2 cut(s) 3046, 3078
BpmI CTGGAG 3 cut(s) 632, 1665, 2688
BpuEI CTTGAG 5 cut(s) 146, 1283, 1574, 2364, 3264
BpuMI CCSGG 6 cut(s) 268, 385, 440, 554, 555, 3649
Bsa29I ATCGAT 1 cut(s) 2036
BsaAI YACGTR 1 cut(s) 971
BsaBI GATNNNNATC 2 cut(s) 44, 2129
BsaHI GRCGYC 3 cut(s) 336, 398, 789
BsaI GGTCTC 2 cut(s) 891, 3786
BsaJI CCNNGG 9 cut(s) 553, 554, 881, 1420, 1803, 2931, 2991, 3066, 3936
BsaWI WCCGGW 4 cut(s) 225, 750, 776, 2570
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 757, 787
Bsc4I CCNNNNNNNGG 8 cut(s) 225, 391, 439, 1162, 2867, 3648, 3994, 4000
Bse118I RCCGGY 8 cut(s) 373, 400, 465, 481, 715, 776, 1155, 3641
Bse1I ACTGG 8 cut(s) 937, 1253, 1273, 1682, 2013, 2714, 3916, 3976
Bse21I CCTNAGG 1 cut(s) 1209
Bse3DI GCAATG 7 cut(s) 455, 1888, 2475, 3388, 3484, 3679, 3816
Bse8I GATNNNNATC 2 cut(s) 44, 2129
BseAI TCCGGA 2 cut(s) 225, 2570
BseBI CCWGG 6 cut(s) 883, 1422, 1805, 1919, 3370, 3995
BseCI ATCGAT 1 cut(s) 2036
BseDI CCNNGG 9 cut(s) 553, 554, 881, 1420, 1803, 2931, 2991, 3066, 3936
BseJI GATNNNNATC 2 cut(s) 44, 2129
BseLI CCNNNNNNNGG 8 cut(s) 225, 391, 439, 1162, 2867, 3648, 3994, 4000
BseMI GCAATG 7 cut(s) 455, 1888, 2475, 3388, 3484, 3679, 3816
BseMII CTCAG 9 cut(s) 978, 1752, 2181, 2256, 2493, 2506, 2811, 3032, 3640
BseNI ACTGG 8 cut(s) 937, 1253, 1273, 1682, 2013, 2714, 3916, 3976
BseRI GAGGAG 9 cut(s) 95, 223, 248, 251, 284, 287, 425, 428, 1025
BseSI GKGCMC 2 cut(s) 2163, 2333
BseX3I CGGCCG 1 cut(s) 54
BseYI CCCAGC 1 cut(s) 1765
BsgI GTGCAG 2 cut(s) 1557, 3135
Bsh1236I CGCG 2 cut(s) 59, 2637
Bsh1285I CGRYCG 3 cut(s) 57, 333, 470
BshNI GGYRCC 1 cut(s) 397
BshTI ACCGGT 1 cut(s) 776
BshVI ATCGAT 1 cut(s) 2036
BsiEI CGRYCG 3 cut(s) 57, 333, 470
BsiHKAI GWGCWC 1 cut(s) 420
BsiHKCI CYCGRG 3 cut(s) 353, 553, 2990
BslFI GGGAC 3 cut(s) 475, 1209, 1667
BslI CCNNNNNNNGG 8 cut(s) 225, 391, 439, 1162, 2867, 3648, 3994, 4000
BsmAI GTCTC 6 cut(s) 70, 891, 1259, 1770, 2495, 3786
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 3 cut(s) 475, 1209, 1667
BsmI GAATGC 4 cut(s) 1015, 1893, 3255, 3684
Bso31I GGTCTC 2 cut(s) 891, 3786
BsoBI CYCGRG 3 cut(s) 353, 553, 2990
Bsp120I GGGCCC 1 cut(s) 2329
Bsp1286I GDGCHC 3 cut(s) 420, 2163, 2333
Bsp13I TCCGGA 2 cut(s) 225, 2570
BspCNI CTCAG 9 cut(s) 979, 1753, 2182, 2257, 2494, 2505, 2810, 3033, 3639
BspDI ATCGAT 1 cut(s) 2036
BspEI TCCGGA 2 cut(s) 225, 2570
BspFNI CGCG 2 cut(s) 59, 2637
BspHI TCATGA 1 cut(s) 3409
BspLI GGNNCC 4 cut(s) 399, 2331, 3511, 3646
BspMAI CTGCAG 3 cut(s) 1999, 2122, 2168
BspMI ACCTGC 2 cut(s) 1334, 3707
BspQI GCTCTTC 2 cut(s) 425, 2780
BspT107I GGYRCC 1 cut(s) 397
BspTNI GGTCTC 2 cut(s) 891, 3786
BsrBI CCGCTC 2 cut(s) 327, 582
BsrDI GCAATG 7 cut(s) 455, 1888, 2475, 3388, 3484, 3679, 3816
BsrFI RCCGGY 8 cut(s) 373, 400, 465, 481, 715, 776, 1155, 3641
BsrI ACTGG 8 cut(s) 937, 1253, 1273, 1682, 2013, 2714, 3916, 3976
BssAI RCCGGY 8 cut(s) 373, 400, 465, 481, 715, 776, 1155, 3641
BssECI CCNNGG 9 cut(s) 553, 554, 881, 1420, 1803, 2931, 2991, 3066, 3936
BssNI GRCGYC 3 cut(s) 336, 398, 789
BssSI CACGAG 1 cut(s) 2744
BssT1I CCWWGG 3 cut(s) 2931, 3066, 3936
Bst2BI CACGAG 1 cut(s) 2744
Bst2UI CCWGG 6 cut(s) 883, 1422, 1805, 1919, 3370, 3995
Bst4CI ACNGT 8 cut(s) 595, 695, 810, 1747, 2241, 3341, 3595, 3923
Bst6I CTCTTC 4 cut(s) 425, 990, 2103, 2780
BstACI GRCGYC 3 cut(s) 336, 398, 789
BstAPI GCANNNNNTGC 1 cut(s) 478
BstBAI YACGTR 1 cut(s) 971
BstFNI CGCG 2 cut(s) 59, 2637
BstH2I RGCGCY 4 cut(s) 219, 380, 401, 1627
BstHHI GCGC 4 cut(s) 218, 379, 400, 1626
BstMAI GTCTC 6 cut(s) 70, 891, 1259, 1770, 2495, 3786
BstMCI CGRYCG 3 cut(s) 57, 333, 470
BstNI CCWGG 6 cut(s) 883, 1422, 1805, 1919, 3370, 3995
BstNSI RCATGY 2 cut(s) 538, 2817
BstPAI GACNNNNGTC 2 cut(s) 883, 2388
BstSFI CTRYAG 3 cut(s) 1995, 2118, 2164
BstSLI GKGCMC 2 cut(s) 2163, 2333
BstUI CGCG 2 cut(s) 59, 2637
BstV2I GAAGAC 5 cut(s) 883, 993, 1423, 2120, 2949
BstX2I RGATCY 3 cut(s) 357, 474, 3509
BstXI CCANNNNNNTGG 2 cut(s) 2750, 3221
BstYI RGATCY 3 cut(s) 357, 474, 3509
BstZI CGGCCG 1 cut(s) 54
Bsu15I ATCGAT 1 cut(s) 2036
Bsu36I CCTNAGG 1 cut(s) 1209
BsuI GTATCC 3 cut(s) 909, 2865, 2929
BsuTUI ATCGAT 1 cut(s) 2036
BtgZI GCGATG 2 cut(s) 187, 500
BtsI GCAGTG 7 cut(s) 874, 2004, 2161, 2546, 3163, 3303, 3747
BveI ACCTGC 2 cut(s) 1334, 3707
CaiI CAGNNNCTG 2 cut(s) 478, 1172
CciI TCATGA 1 cut(s) 3409
CfoI GCGC 4 cut(s) 218, 379, 400, 1626
Cfr10I RCCGGY 8 cut(s) 373, 400, 465, 481, 715, 776, 1155, 3641
Cfr13I GGNCC 8 cut(s) 88, 228, 269, 1915, 2329, 2330, 2589, 3366
Cfr9I CCCGGG 1 cut(s) 553
ClaI ATCGAT 1 cut(s) 2036
CseI GACGC 2 cut(s) 344, 797
CsiI ACCWGGT 1 cut(s) 1917
Csp6I GTAC 9 cut(s) 142, 454, 774, 811, 1236, 1356, 2340, 2840, 3630
CspAI ACCGGT 1 cut(s) 776
CviQI GTAC 9 cut(s) 142, 454, 774, 811, 1236, 1356, 2340, 2840, 3630
DinI GGCGCC 1 cut(s) 399
EaeI YGGCCR 5 cut(s) 54, 934, 3211, 3438, 3985
EagI CGGCCG 1 cut(s) 54
Eam1104I CTCTTC 4 cut(s) 425, 990, 2103, 2780
EarI CTCTTC 4 cut(s) 425, 990, 2103, 2780
EciI GGCGGA 2 cut(s) 107, 230
Ecl136II GAGCTC 1 cut(s) 418
EclXI CGGCCG 1 cut(s) 54
Eco130I CCWWGG 3 cut(s) 2931, 3066, 3936
Eco147I AGGCCT 1 cut(s) 3039
Eco24I GRGCYC 2 cut(s) 420, 2333
Eco31I GGTCTC 2 cut(s) 891, 3786
Eco47I GGWCC 3 cut(s) 88, 228, 1915
Eco47III AGCGCT 1 cut(s) 1625
Eco52I CGGCCG 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 6 cut(s) 623, 849, 1417, 2351, 2961, 3751
Eco72I CACGTG 1 cut(s) 971
Eco81I CCTNAGG 1 cut(s) 1209
Eco88I CYCGRG 3 cut(s) 353, 553, 2990
EcoICRI GAGCTC 1 cut(s) 418
EcoO109I RGGNCCY 4 cut(s) 2329, 2330, 2589, 3366
EcoRI GAATTC 2 cut(s) 562, 746
EcoRII CCWGG 6 cut(s) 881, 1420, 1803, 1917, 3368, 3993
EcoT14I CCWWGG 3 cut(s) 2931, 3066, 3936
EcoT38I GRGCYC 2 cut(s) 420, 2333
EgeI GGCGCC 1 cut(s) 399
EheI GGCGCC 1 cut(s) 399
ErhI CCWWGG 3 cut(s) 2931, 3066, 3936
Esp3I CGTCTC 1 cut(s) 70
FalI AAGNNNNNCTT 2 cut(s) 1290, 1322
FaqI GGGAC 3 cut(s) 475, 1209, 1667
FauI CCCGC 3 cut(s) 575, 1464, 3798
FauNDI CATATG 1 cut(s) 2844
FblI GTMKAC 2 cut(s) 2407, 2755
FriOI GRGCYC 2 cut(s) 420, 2333
GlaI GCGC 4 cut(s) 217, 378, 399, 1625
GsaI CCCAGC 1 cut(s) 1769
GsuI CTGGAG 3 cut(s) 632, 1665, 2688
HaeII RGCGCY 4 cut(s) 219, 380, 401, 1627
HgaI GACGC 2 cut(s) 344, 797
HhaI GCGC 4 cut(s) 218, 379, 400, 1626
Hin1I GRCGYC 3 cut(s) 336, 398, 789
Hin6I GCGC 4 cut(s) 216, 377, 398, 1624
HinP1I GCGC 4 cut(s) 216, 377, 398, 1624
HincII GTYRAC 2 cut(s) 1810, 3789
HindII GTYRAC 2 cut(s) 1810, 3789
HphI GGTGA 4 cut(s) 791, 1486, 1555, 3436
Hpy166II GTNNAC 9 cut(s) 430, 1200, 1810, 1924, 2408, 2562, 2756, 3347, 3789
Hpy8I GTNNAC 9 cut(s) 430, 1200, 1810, 1924, 2408, 2562, 2756, 3347, 3789
Hpy99I CGWCG 5 cut(s) 37, 139, 338, 518, 521
HpyCH4III ACNGT 8 cut(s) 595, 695, 810, 1747, 2241, 3341, 3595, 3923
HpyCH4IV ACGT 5 cut(s) 498, 519, 970, 1390, 2605
HpySE526I ACGT 5 cut(s) 498, 519, 970, 1390, 2605
Hsp92I GRCGYC 3 cut(s) 336, 398, 789
HspAI GCGC 4 cut(s) 216, 377, 398, 1624
KasI GGCGCC 1 cut(s) 397
Kpn2I TCCGGA 2 cut(s) 225, 2570
KroI GCCGGC 3 cut(s) 373, 481, 715
KroNI GCCGGC 3 cut(s) 375, 483, 717
LguI GCTCTTC 2 cut(s) 425, 2780
LmnI GCTCC 8 cut(s) 120, 261, 415, 607, 1012, 2203, 2879, 3650
LweI GCATC 3 cut(s) 357, 3569, 3937
MabI ACCWGGT 1 cut(s) 1917
MaeII ACGT 5 cut(s) 498, 519, 970, 1390, 2605
MaeIII GTNAC 5 cut(s) 1255, 2544, 3442, 3613, 3967
MbiI CCGCTC 2 cut(s) 327, 582
MfeI CAATTG 1 cut(s) 1527
MflI RGATCY 3 cut(s) 357, 474, 3509
MhlI GDGCHC 3 cut(s) 420, 2163, 2333
MlsI TGGCCA 2 cut(s) 936, 3213
MluI ACGCGT 1 cut(s) 2635
MluNI TGGCCA 2 cut(s) 936, 3213
Mly113I GGCGCC 1 cut(s) 398
MlyI GAGTC 5 cut(s) 1007, 1604, 1805, 1919, 3101
MmeI TCCRAC 3 cut(s) 171, 708, 3708
Mox20I TGGCCA 2 cut(s) 936, 3213
MroI TCCGGA 2 cut(s) 225, 2570
MroNI GCCGGC 3 cut(s) 373, 481, 715
MroXI GAANNNNTTC 1 cut(s) 3141
MscI TGGCCA 2 cut(s) 936, 3213
MslI CAYNNNNRTG 3 cut(s) 11, 1070, 3219
Msp20I TGGCCA 2 cut(s) 936, 3213
MspA1I CMGCKG 1 cut(s) 1994
MunI CAATTG 1 cut(s) 1527
Mva1269I GAATGC 4 cut(s) 1015, 1893, 3255, 3684
MvaI CCWGG 6 cut(s) 883, 1422, 1805, 1919, 3370, 3995
MvnI CGCG 2 cut(s) 59, 2637
NaeI GCCGGC 3 cut(s) 375, 483, 717
NarI GGCGCC 1 cut(s) 398
NciI CCSGG 6 cut(s) 268, 385, 440, 554, 555, 3649
NdeI CATATG 1 cut(s) 2844
NgoMIV GCCGGC 3 cut(s) 373, 481, 715
NlaIV GGNNCC 4 cut(s) 399, 2331, 3511, 3646
NmeAIII GCCGAG 2 cut(s) 235, 2866
NmuCI GTSAC 3 cut(s) 1255, 2544, 3442
NspI RCATGY 2 cut(s) 538, 2817
PaeI GCATGC 1 cut(s) 2817
PaeR7I CTCGAG 1 cut(s) 353
PagI TCATGA 1 cut(s) 3409
PceI AGGCCT 1 cut(s) 3039
PciI ACATGT 1 cut(s) 534
PciSI GCTCTTC 2 cut(s) 425, 2780
PcsI WCGNNNNNNNCGW 2 cut(s) 504, 513
PctI GAATGC 4 cut(s) 1015, 1893, 3255, 3684
PdiI GCCGGC 3 cut(s) 375, 483, 717
PdmI GAANNNNTTC 1 cut(s) 3141
PfoI TCCNGGA 1 cut(s) 3647
PinAI ACCGGT 1 cut(s) 776
Ple19I CGATCG 1 cut(s) 333
PleI GAGTC 5 cut(s) 1006, 1603, 1805, 1919, 3101
PluTI GGCGCC 1 cut(s) 401
PmaCI CACGTG 1 cut(s) 971
PmlI CACGTG 1 cut(s) 971
PpsI GAGTC 5 cut(s) 1006, 1603, 1805, 1919, 3101
Ppu21I YACGTR 1 cut(s) 971
PscI ACATGT 1 cut(s) 534
PshAI GACNNNNGTC 2 cut(s) 883, 2388
PshBI ATTAAT 1 cut(s) 1689
PsiI TTATAA 1 cut(s) 2628
Psp124BI GAGCTC 1 cut(s) 420
Psp6I CCWGG 6 cut(s) 881, 1420, 1803, 1917, 3368, 3993
PspCI CACGTG 1 cut(s) 971
PspFI CCCAGC 1 cut(s) 1765
PspGI CCWGG 6 cut(s) 881, 1420, 1803, 1917, 3368, 3993
PspN4I GGNNCC 4 cut(s) 399, 2331, 3511, 3646
PspOMI GGGCCC 1 cut(s) 2329
PspPI GGNCC 8 cut(s) 88, 228, 269, 1915, 2329, 2330, 2589, 3366
PsrI GAACNNNNNNTAC 2 cut(s) 3613, 3645
PstI CTGCAG 3 cut(s) 1999, 2122, 2168
PstNI CAGNNNCTG 2 cut(s) 478, 1172
PsuI RGATCY 3 cut(s) 357, 474, 3509
PvuI CGATCG 1 cut(s) 333
PvuII CAGCTG 1 cut(s) 1994
RsaI GTAC 9 cut(s) 143, 455, 775, 812, 1237, 1357, 2341, 2841, 3631
RsaNI GTAC 9 cut(s) 142, 454, 774, 811, 1236, 1356, 2340, 2840, 3630
RseI CAYNNNNRTG 3 cut(s) 11, 1070, 3219
SacI GAGCTC 1 cut(s) 420
SapI GCTCTTC 2 cut(s) 425, 2780
Sau96I GGNCC 8 cut(s) 88, 228, 269, 1915, 2329, 2330, 2589, 3366
ScaI AGTACT 1 cut(s) 143
SchI GAGTC 5 cut(s) 1007, 1604, 1805, 1919, 3101
SduI GDGCHC 3 cut(s) 420, 2163, 2333
SexAI ACCWGGT 1 cut(s) 1917
SfaNI GCATC 3 cut(s) 357, 3569, 3937
SfcI CTRYAG 3 cut(s) 1995, 2118, 2164
SfoI GGCGCC 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 353
SgrAI CRCCGGYG 1 cut(s) 400
SinI GGWCC 3 cut(s) 88, 228, 1915
SlaI CTCGAG 1 cut(s) 353
SmaI CCCGGG 1 cut(s) 555
SmiMI CAYNNNNRTG 3 cut(s) 11, 1070, 3219
SmlI CTYRAG 6 cut(s) 161, 353, 1262, 1589, 2379, 3279
SmoI CTYRAG 6 cut(s) 161, 353, 1262, 1589, 2379, 3279
SpeI ACTAGT 2 cut(s) 26, 1232
SphI GCATGC 1 cut(s) 2817
SseBI AGGCCT 1 cut(s) 3039
SspDI GGCGCC 1 cut(s) 397
SspI AATATT 3 cut(s) 2919, 3204, 3570
SstI GAGCTC 1 cut(s) 420
StuI AGGCCT 1 cut(s) 3039
StyI CCWWGG 3 cut(s) 2931, 3066, 3936
TaaI ACNGT 8 cut(s) 595, 695, 810, 1747, 2241, 3341, 3595, 3923
TaiI ACGT 5 cut(s) 501, 522, 973, 1393, 2608
TaqI TCGA 9 cut(s) 329, 333, 354, 411, 524, 560, 572, 2036, 2850
TatI WGTACW 2 cut(s) 141, 2339
TseFI GTSAC 3 cut(s) 1255, 2544, 3442
Tsp45I GTSAC 3 cut(s) 1255, 2544, 3442
TspGWI ACGGA 5 cut(s) 404, 819, 1927, 2447, 3180
TspMI CCCGGG 1 cut(s) 553
VpaK11BI GGWCC 3 cut(s) 88, 228, 1915
VspI ATTAAT 1 cut(s) 1689
XbaI TCTAGA 1 cut(s) 2683
XceI RCATGY 2 cut(s) 538, 2817
XcmI CCANNNNNNNNNTGG 1 cut(s) 1187
XhoI CTCGAG 1 cut(s) 353
XmaI CCCGGG 1 cut(s) 553
XmiI GTMKAC 2 cut(s) 2407, 2755
XmnI GAANNNNTTC 1 cut(s) 3141
ZrmI AGTACT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.