Rh6CG444200

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001838
Physical Location & Seq
Reverse (-)
62235086 .. 62240851
5766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG444200.1

Sequence Viewer

Length: 4428 bp
ATGGCTAAGAACTATAGATTTTCAATGGACCAAAAGGACATTGTTAGGGTATTGATTACCACAGTAGATGGTTTTATTCGGGATCAGCTGATCAATAAAGAACAGAGAAGCCAACACAGGGAACAGTGTGCTGAAAGATTGGCAGCTGAAGATGGATCCTGTGGTAAAGAAACCGAGGTTCGTTACTCTGATCAAGCAGTGCTAGCAAATCTGGATTGGGGTATTGAGGCTCTTGAAGAAGCAATCGACACTTCAAATATGGAAACTAAGCTTGCAAGATTAGACCATGCTGAAAAAATGTTGCAAGTTTGTGCCATGCTGAATTCTGATCAGAAGACTGCTGGAGTCCCCAATTTTTACCTCTCAGCTTGGGCACACCTCAACTTAGCATACCTGTGGAAATTGAGGAATAACATCCAAAACTCAGTTCTCCATGTCATTGAGATGTTTACAGTTGATCCTTTTTTCTCACGGATAGATTTTGCTCCTGCAATCTGGAAACTTTTGTTTCTTCCACACATGAGCTCAATTATAGGGTGGTATTCGGAGCAGAGGCACCGCCTTGTGATGGAAGTAATTCCTGATTCTCATGATCTGTCTTTCACAGCTGATCTTGATCAATTTTTCAATGAGTCTTTAATCTTTTCAATGAGGCCGGATCAGATAGAGAAATTGCAAAAGCTGGAGCAGCTTTATGGTGAGTCACTGGATGAGAACACAAGGCTTTATGCCAAGTACTTCAAGGATTGCATGACACCTGATTCAACCTCCAGTAAGAAAGTGATTCCAATGTTGCCAATTGCTGAGCCACCAATGACTCCATTGCATGAGGTCAGCCGCTCGATTCCTGATTTTGTCAAGTTTGGTCCTATCTTGCCCAAGAGTGCTGGCTTTTCTCCCATCCTCAAGTCTAAAGATGGCACAAGAGACGTAAACAGAATGAATCTAAGTTCTGCATCTTCACAAAATTTGGAGCCTGTCAGATGGGATCCTCAGGAATGTATCCCCGAGGAGAACGAAGATGAATCTGATTATGAGCCAAATGATGCTAATATGGCTTCTGATCACGAGAAGGAAACTGGAGGAAAAGTACAGTCATCTGTACTCAGGAGTCGAGTACATAGCCCAACAATCTTTTCTCCACTAATTTCTCCTAAAACTTCACCAAAAGTTTCATCTCCAAAACCAGACACAGCTGCTTCTGCATTGCGTTTACTGTCTGTCAAAATCACAGATTCAGCTGTTGCCACCTCTTTGCCTGCATCTCCGGGCATGAGCAATGATTACAGCATCAGCTCTGCAGACTCGGATGTTGAAGTGATTGAGACTACCAAAAGTTGCAGGAAAGTCTATAGCCGAACATCAAGCATGAATAATGAGCTTGTGAAAATGTCGAAAAATAGTCCACCTAATGAAAATGATGAAGGAGGCCAGAGCTGTGTTTCACTTCCATCTTCTGACAGGATGACTACTAAGTCAAGACCACCTAAGGATTTTGTTTGTCCTATCACTGGCCAGATATTTATTGATCCAGTTACTCTTGAAACAGGTCAGACATACGAAAGAAAAGCAATCCAAGAATGGCTGAAACGAGGAAACACAACCTGTCCCATTACGCGGCAACCCATCTCTACAACTACTCAGCTGCCTAAAACAAACTATGTTTTGAAAAGACTAATAACCTCTTGGAAAGAACAGCATCCTGAACTCGCTCAAGAATGTGCATACTACGAAACACCAAGAAACTCATTCCACCGCTCCTCAGTGAAAGAAGTCCATTCTGGTACCACAACTCCACAAAGAACATGTGACTTCATGGGTCATAGGAATACCGATGATTATATCAGCCAAAGGAGCAAAAGGTTTATGCAGGCAGCTGTTTCTACATCACCAACCAGTGTGATATCTCAAGCTGCAGTTGAGACTATTATCAATGGCTTAAAGCCTCATGTTGCATGTCTGTGTACTTCAGATAAACTTCAAGAGTGTGAAACAGCAGTGCTAGAAATAGTCAGATTATGGAAGGACTCAAAGAGTGATCCTGCAATTCATACATTCTTATCGGAGCTGACAACTGTGAATGGATTCATTGAAATACTTTCAGCTTCTCTGAATAGGGAAGTCCTGAGAACATCTATTTACATCCTCTCCGAGCTAATATTTGCCGATGAAAGTGTTGGAGAAACACTTACCAGTGTAGACTCCGACCTTGATTGCCTGGCTGCTCTTTTAAAGAATGGACTAGCTGAGCCTGCTGTTCTCATTTACCAGCTGAGGCCGGTATTTGCTCAGCTTTCAGCTCATGATCTTATACCCTCCCTTGTGCAGCTAATTCAGAGCAAAAATGAGGAATCAAATGATCTTCAATTACTAATGGACCCCAAAGATGCTGCATTAGCTATTCTTGAGCAAGTCTTGATGGGAGGAGATGAAAATAGCAGATCCATTAATGCTTTGAGTGTTTTTTCTGCGAGTGGTATACCTGCTCTAGTTAAGTGTTTAGACAGGCCAGAAGGAAGAAGGTCCATTGTTTCTATTCTATTATGCTGCATGCAAGCTGAAAAAACTTGCAGGAACTTAATAGCAAATAGAATTGAGCTGTCTCCTGTTCTCGAACTATTTCACGCTGGAAGTGATGGTGTGAGAGGCATATGTGTAGAGTTTCTCTCAGAACTGGTACAGTTGAACAGGAGAACATTATGCAATCAGATCTTGCAAACGATTAAAGACGAAGGAGCATTTAGTACAATGCATACCTTTCTTGTATATCTTCAAATGGCTCCAATGGAGCAGCAACCTGCAATTGCTACTCTCCTTCTTCAGCTTGATCTCCTGGTTGAGCCTTGGAAGATGAGCATTTATAGGGAAGAATCTATAGAGGGACTAATTGAGGCCCTCCGAAGGAAAGACTTCTCAAACTCTCAAATGATGGCTTTAGATGCCTTGTTATCTCTTACTGGACGTGTGACTTCATCCGGGGATTCATACACTGAAGCCTGGTTACTCAAGATTGCTGGGTTTGATCAACCTTACAAGGGTTTGATGAAGGCAGAATGGCTAAGAAAACAAGACAATGATTTGATGGAGACAATGGAAGAAGAAGAGAAAGCAGTAAGCTCTTGGCAGAAAAAAGTTGCTTTTGTACTTTGCAACCATGAGAAGGGATCCATTTTTAAAGCTTTGGAAGAATGCCTCAAGAGTAAATCCATTGAGATGGCAAAGTCATGTCTTGTCATAGCTACATGGCTCACACACATGCTTTCTGTACTTCCTGATACAGGTGTGAAAATCAGTGCTCGCAAGGCCTTGCTTGAAGAATTCATAAATGTTCTCCAATCATCGAATAATTTGGAGGAGAAGATTTTAGCAACCCTGGCTTTGAAATCTTTTGTCAGTGAACCAGCTGCACTTGAAGCACTAGGAGTGTATGCTAAATGCATCTACAAAACATTGAGAAAGCTTAAGAGGAGCACTGTGGTGGCGAGTGACATAATGAAAGCGCTGATGAACTTGTCATCTGTTGACGTAACAGAGTTGTGGAGCTGTGCTGAAGTGGTTGAACTAGATTCAAGCTCTAATGGGGAGGTTACATCTATACTTCATCTAAAAGGCCGTGTTTTAAGCAGCCATTCTGATGGAACTATGAAGGTATGGGATGCTGGCAAGAAAGTATTAAGACTGATTCAAGAAGTCCGCGAGCACACAAAAGCTGTCACATGCCTCTATATTTCACCTTCAGGTGATAAATTATACAGTGGTTCGTTAGACAAAACTATTCGGGTGTGGGCAATCAAAGCAGAAGAAATTCATTGCCTTCAGGTTCACGATGTGAAAGAGGTAGTATATGAGTTGGTTGCAAATGCTAAAGTGGCGTGCTTCATTTCTCAAGGCACTGGAGTGAAGGTTTACGAATGGTCTGGGGTCCAAAAACATGTAAACTTCAACAAATATGTAAAGAGCCTAGCCATGTCAGGGAACAGCCTCTATTGTGGTTGCTCTGGTTACAGCATCCAGGAGATCAACTTGGGGAGACATATATCAAGCACATTCTACTCCGGCACAAGAAAGCTGTTGGGAAAACAGATGTTCTATTCCCTTGAGATTCATGATGGCATCCTCTATGCGGGCGGTTCATCAGTTGATGCAACAGCTGGGAAGATATTTTCGCTTCCAAACAAGGCGGTATTAGGATCATTCTCAACTGGATTTGACATCCAACGCATTGCCACCAACAATGACTTGATCTTTACGGCAACAAAGTGTGGGATAATTGAGGTTTGGTTGAAAGAGAGGTTCACAAGAATTGCTTCCATCAAAACAGCTGGCGGCGGTCATGCCAAGATCACATCCTTGGCGGCAGATATGGACGGAGGATTGCTGTTTGCAGGTTCCTCGGATGGCAGAATCCAGGTTTGGGCACTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1475

Amino Acids

164.11

Weight (kDa)

5.83

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 9 - 268 2.3e-74 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 496 - 567 1.6e-15 U-box domain
ARM_LIN_2nd PF23654 642 - 803 5.6e-61 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 804 - 1166 3e-115 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR3_1st PF25173 1184 - 1324 3.8e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 1192 - 1268 7.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 1196 - 1289 4.8e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 1201 - 1303 6.7e-10 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1203 - 1269 1.3e-09 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1203 - 1293 4.8e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 1205 - 1267 2.9e-08 WDR3 second beta-propeller domain
WD40 PF00400 1227 - 1262 5.3e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1474
Acc36I ACCTGC 3 cut(s) 2491, 2806, 4376
Acc65I GGTACC 1 cut(s) 1784
AccB1I GGYRCC 2 cut(s) 555, 1784
AccBSI CCGCTC 2 cut(s) 840, 1758
AccI GTMKAC 2 cut(s) 2199, 2479
AccII CGCG 2 cut(s) 1618, 3696
AcoI YGGCCR 1 cut(s) 1513
AcsI RAATTY 4 cut(s) 322, 967, 3317, 3804
AcuI CTGAAG 7 cut(s) 168, 1953, 2804, 3012, 3570, 3720, 3800
AdeI CACNNNGTG 1 cut(s) 3829
AfeI AGCGCT 1 cut(s) 3501
AflII CTTAAG 1 cut(s) 3461
AflIII ACRYGT 3 cut(s) 1805, 2962, 3931
AjiI CACGTC 1 cut(s) 2963
AjnI CCWGG 6 cut(s) 2217, 2832, 2996, 3372, 4011, 4407
AjuI GAANNNNNNNTTGG 6 cut(s) 411, 443, 1120, 1152, 4396, 4428
AleI CACNNNNGTG 2 cut(s) 3476, 3896
Alw21I GWGCWC 4 cut(s) 527, 3298, 3473, 3702
Alw26I GTCTC 6 cut(s) 921, 1319, 1916, 2607, 3080, 4024
Ama87I CYCGRG 1 cut(s) 1007
Aor51HI AGCGCT 1 cut(s) 3501
AoxI GGCC 8 cut(s) 653, 1429, 1513, 2276, 2507, 2892, 3303, 3610
ApoI RAATTY 4 cut(s) 322, 967, 3317, 3804
AseI ATTAAT 1 cut(s) 2448
Asp700I GAANNNNTTC 6 cut(s) 576, 2084, 2619, 2909, 3804, 4306
Asp718I GGTACC 1 cut(s) 1784
AspLEI GCGC 1 cut(s) 3502
AspS9I GGNCC 6 cut(s) 28, 866, 2377, 2523, 2893, 3922
AsuC2I CCSGG 2 cut(s) 1269, 2977
AsuHPI GGTGA 5 cut(s) 710, 1155, 1881, 3723, 3752
AsuNHI GCTAGC 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 1007
AvaII GGWCC 5 cut(s) 28, 866, 2377, 2523, 3922
AxyI CCTNAGG 2 cut(s) 993, 1488
BaeGI GKGCMC 2 cut(s) 376, 4420
BaeI ACNNNNGTAYC 2 cut(s) 3267, 3300
BalI TGGCCA 1 cut(s) 1515
BamHI GGATCC 3 cut(s) 155, 988, 3164
BanI GGYRCC 2 cut(s) 555, 1784
BanII GRGCYC 1 cut(s) 527
BarI GAAGNNNNNNTAC 2 cut(s) 2985, 3017
BauI CACGAG 1 cut(s) 1067
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 4 cut(s) 527, 3298, 3473, 3702
BbvCI CCTCAGC 1 cut(s) 2273
BceAI ACGGC 2 cut(s) 3597, 4266
BcgI CGANNNNNNTGC 2 cut(s) 4374, 4408
BciT130I CCWGG 6 cut(s) 2219, 2834, 2998, 3374, 4013, 4409
BciVI GTATCC 1 cut(s) 1013
BclI TGATCA 6 cut(s) 90, 190, 328, 616, 1063, 3022
BcnI CCSGG 2 cut(s) 1269, 2977
BcoDI GTCTC 6 cut(s) 921, 1319, 1916, 2607, 3080, 4024
BfaI CTAG 8 cut(s) 203, 2003, 2243, 2489, 3419, 3563, 3962, 4421
BfmI CTRYAG 5 cut(s) 13, 1299, 1351, 1914, 2874
BfoI RGCGCY 1 cut(s) 3503
BfrI CTTAAG 1 cut(s) 3461
BfuAI ACCTGC 3 cut(s) 2491, 2806, 4376
BfuI GTATCC 1 cut(s) 1013
BglII AGATCT 1 cut(s) 2709
BlpI GCTNAGC 3 cut(s) 804, 2247, 2289
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 8 cut(s) 1269, 2219, 2834, 2977, 2998, 3374, 4013, 4409
Bme18I GGWCC 5 cut(s) 28, 866, 2377, 2523, 3922
BmeT110I CYCGRG 1 cut(s) 1007
BmgBI CACGTC 1 cut(s) 2963
BmgT120I GGNCC 6 cut(s) 28, 866, 2377, 2523, 2893, 3922
BmrFI CCNGG 8 cut(s) 1269, 2219, 2834, 2977, 2998, 3374, 4013, 4409
BmtI GCTAGC 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 341
BplI GAGNNNNNCTC 2 cut(s) 2651, 2683
BpmI CTGGAG 5 cut(s) 363, 704, 754, 1101, 3915
Bpu10I CCTNAGC 1 cut(s) 2273
Bpu1102I GCTNAGC 3 cut(s) 804, 2247, 2289
BpuEI CTTGAG 8 cut(s) 890, 1698, 1893, 2426, 2990, 3179, 3870, 4118
BpuMI CCSGG 2 cut(s) 1269, 2977
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 7 cut(s) 174, 1008, 2843, 2976, 3372, 4350, 4392
BsaXI ACNNNNNCTCC 8 cut(s) 1074, 1104, 1777, 1807, 2132, 2162, 3888, 3918
Bse118I RCCGGY 1 cut(s) 2278
Bse21I CCTNAGG 2 cut(s) 993, 1488
Bse3DI GCAATG 5 cut(s) 821, 1205, 1285, 3808, 4221
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 6 cut(s) 2219, 2834, 2998, 3374, 4013, 4409
BseDI CCNNGG 7 cut(s) 174, 1008, 2843, 2976, 3372, 4350, 4392
BseJI GATNNNNATC 1 cut(s) 615
BseMI GCAATG 5 cut(s) 821, 1205, 1285, 3808, 4221
BseRI GAGGAG 5 cut(s) 1025, 1750, 2439, 3368, 3481
BseSI GKGCMC 2 cut(s) 376, 4420
BseYI CCCAGC 2 cut(s) 3014, 4151
BsgI GTGCAG 2 cut(s) 2345, 3390
Bsh1236I CGCG 2 cut(s) 1618, 3696
BshFI GGCC 8 cut(s) 655, 1431, 1515, 2278, 2509, 2894, 3305, 3612
BshNI GGYRCC 2 cut(s) 555, 1784
BsiHKAI GWGCWC 4 cut(s) 527, 3298, 3473, 3702
BsiHKCI CYCGRG 1 cut(s) 1007
BsiSI CCGG 5 cut(s) 656, 1268, 2279, 2976, 4056
BslFI GGGAC 3 cut(s) 332, 1593, 2895
BsmAI GTCTC 6 cut(s) 921, 1319, 1916, 2607, 3080, 4024
BsmBI CGTCTC 1 cut(s) 921
BsmFI GGGAC 3 cut(s) 332, 1593, 2895
BsmI GAATGC 1 cut(s) 3194
BsnI GGCC 8 cut(s) 655, 1431, 1515, 2278, 2509, 2894, 3305, 3612
BsoBI CYCGRG 1 cut(s) 1007
Bsp1286I GDGCHC 6 cut(s) 376, 527, 3298, 3473, 3702, 4420
Bsp1720I GCTNAGC 3 cut(s) 804, 2247, 2289
BspANI GGCC 8 cut(s) 655, 1431, 1515, 2278, 2509, 2894, 3305, 3612
BspFNI CGCG 2 cut(s) 1618, 3696
BspHI TCATGA 3 cut(s) 589, 2302, 4105
BspMAI CTGCAG 2 cut(s) 1303, 1918
BspMI ACCTGC 3 cut(s) 2491, 2806, 4376
BspOI GCTAGC 1 cut(s) 206
BspT107I GGYRCC 2 cut(s) 555, 1784
BspTI CTTAAG 1 cut(s) 3461
BsrBI CCGCTC 2 cut(s) 840, 1758
BsrDI GCAATG 5 cut(s) 821, 1205, 1285, 3808, 4221
BsrFI RCCGGY 1 cut(s) 2278
BssAI RCCGGY 1 cut(s) 2278
BssECI CCNNGG 7 cut(s) 174, 1008, 2843, 2976, 3372, 4350, 4392
BssNAI GTATAC 1 cut(s) 2480
BssSI CACGAG 1 cut(s) 1067
BssT1I CCWWGG 2 cut(s) 2843, 4350
Bst1107I GTATAC 1 cut(s) 2480
Bst2BI CACGAG 1 cut(s) 1067
Bst2UI CCWGG 6 cut(s) 2219, 2834, 2998, 3374, 4013, 4409
Bst4CI ACNGT 9 cut(s) 64, 126, 454, 1095, 1218, 2077, 2682, 3475, 3755
Bst6I CTCTTC 1 cut(s) 3096
BstAFI CTTAAG 1 cut(s) 3461
BstENI CCTNNNNNAGG 1 cut(s) 3276
BstFNI CGCG 2 cut(s) 1618, 3696
BstH2I RGCGCY 1 cut(s) 3503
BstHHI GCGC 1 cut(s) 3502
BstMAI GTCTC 6 cut(s) 921, 1319, 1916, 2607, 3080, 4024
BstNI CCWGG 6 cut(s) 2219, 2834, 2998, 3374, 4013, 4409
BstNSI RCATGY 6 cut(s) 1809, 1959, 2554, 3259, 3720, 3935
BstSCI CCNGG 8 cut(s) 1267, 2217, 2832, 2975, 2996, 3372, 4011, 4407
BstSFI CTRYAG 5 cut(s) 13, 1299, 1351, 1914, 2874
BstSLI GKGCMC 2 cut(s) 376, 4420
BstUI CGCG 2 cut(s) 1618, 3696
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 5 cut(s) 155, 988, 2441, 2709, 3164
BstXI CCANNNNNNTGG 2 cut(s) 3214, 3635
BstYI RGATCY 5 cut(s) 155, 988, 2441, 2709, 3164
BstZ17I GTATAC 1 cut(s) 2480
Bsu36I CCTNAGG 2 cut(s) 993, 1488
BsuI GTATCC 1 cut(s) 1013
BsuRI GGCC 8 cut(s) 655, 1431, 1515, 2278, 2509, 2894, 3305, 3612
BtrI CACGTC 1 cut(s) 2963
BtsI GCAGTG 2 cut(s) 204, 2004
BveI ACCTGC 3 cut(s) 2491, 2806, 4376
CciI TCATGA 3 cut(s) 589, 2302, 4105
CfoI GCGC 1 cut(s) 3502
Cfr10I RCCGGY 1 cut(s) 2278
Cfr13I GGNCC 6 cut(s) 28, 866, 2377, 2523, 2893, 3922
DraI TTTAAA 2 cut(s) 2232, 3175
DraIII CACNNNGTG 1 cut(s) 3829
DrdI GACNNNNNNGTC 1 cut(s) 1474
DseDI GACNNNNNNGTC 1 cut(s) 1474
EaeI YGGCCR 1 cut(s) 1513
Eam1104I CTCTTC 1 cut(s) 3096
EarI CTCTTC 1 cut(s) 3096
Ecl136II GAGCTC 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 2843, 4350
Eco147I AGGCCT 1 cut(s) 3305
Eco24I GRGCYC 1 cut(s) 527
Eco32I GATATC 1 cut(s) 1905
Eco47I GGWCC 5 cut(s) 28, 866, 2377, 2523, 3922
Eco47III AGCGCT 1 cut(s) 3501
Eco53kI GAGCTC 1 cut(s) 525
Eco57I CTGAAG 7 cut(s) 168, 1953, 2804, 3012, 3570, 3720, 3800
Eco81I CCTNAGG 2 cut(s) 993, 1488
Eco88I CYCGRG 1 cut(s) 1007
EcoICRI GAGCTC 1 cut(s) 525
EcoNI CCTNNNNNAGG 1 cut(s) 3276
EcoO109I RGGNCCY 1 cut(s) 2893
EcoRI GAATTC 2 cut(s) 322, 3317
EcoRII CCWGG 6 cut(s) 2217, 2832, 2996, 3372, 4011, 4407
EcoRV GATATC 1 cut(s) 1905
EcoT14I CCWWGG 2 cut(s) 2843, 4350
EcoT22I ATGCAT 2 cut(s) 2754, 3440
EcoT38I GRGCYC 1 cut(s) 527
ErhI CCWWGG 2 cut(s) 2843, 4350
Esp3I CGTCTC 1 cut(s) 921
FalI AAGNNNNNCTT 2 cut(s) 4291, 4323
FaqI GGGAC 3 cut(s) 332, 1593, 2895
FauI CCCGC 1 cut(s) 4117
FauNDI CATATG 1 cut(s) 2651
FbaI TGATCA 6 cut(s) 90, 190, 328, 616, 1063, 3022
FblI GTMKAC 2 cut(s) 2199, 2479
FriOI GRGCYC 1 cut(s) 527
FspBI CTAG 8 cut(s) 203, 2003, 2243, 2489, 3419, 3563, 3962, 4421
GlaI GCGC 1 cut(s) 3501
GsaI CCCAGC 2 cut(s) 3018, 4155
GsuI CTGGAG 5 cut(s) 363, 704, 754, 1101, 3915
HaeII RGCGCY 1 cut(s) 3503
HaeIII GGCC 8 cut(s) 655, 1431, 1515, 2278, 2509, 2894, 3305, 3612
HapII CCGG 5 cut(s) 656, 1268, 2279, 2976, 4056
HhaI GCGC 1 cut(s) 3502
Hin6I GCGC 1 cut(s) 3500
HinP1I GCGC 1 cut(s) 3500
HincII GTYRAC 1 cut(s) 3523
HindII GTYRAC 1 cut(s) 3523
HindIII AAGCTT 3 cut(s) 269, 3177, 3458
HpaII CCGG 5 cut(s) 656, 1268, 2279, 2976, 4056
HphI GGTGA 5 cut(s) 710, 1155, 1881, 3723, 3752
HpyCH4III ACNGT 9 cut(s) 64, 126, 454, 1095, 1218, 2077, 2682, 3475, 3755
HpyCH4IV ACGT 3 cut(s) 930, 2962, 3525
HpySE526I ACGT 3 cut(s) 930, 2962, 3525
HspAI GCGC 1 cut(s) 3500
KpnI GGTACC 1 cut(s) 1788
Ksp22I TGATCA 6 cut(s) 90, 190, 328, 616, 1063, 3022
MaeI CTAG 8 cut(s) 203, 2003, 2243, 2489, 3419, 3563, 3962, 4421
MaeII ACGT 3 cut(s) 930, 2962, 3525
MbiI CCGCTC 2 cut(s) 840, 1758
MfeI CAATTG 2 cut(s) 798, 2802
MflI RGATCY 5 cut(s) 155, 988, 2441, 2709, 3164
MhlI GDGCHC 6 cut(s) 376, 527, 3298, 3473, 3702, 4420
MlsI TGGCCA 1 cut(s) 1515
MluNI TGGCCA 1 cut(s) 1515
MlyI GAGTC 8 cut(s) 354, 641, 710, 811, 1120, 1298, 2021, 2195
MmeI TCCRAC 3 cut(s) 2158, 2229, 4240
Mox20I TGGCCA 1 cut(s) 1515
Mph1103I ATGCAT 2 cut(s) 2754, 3440
MroXI GAANNNNTTC 6 cut(s) 576, 2084, 2619, 2909, 3804, 4306
MscI TGGCCA 1 cut(s) 1515
MslI CAYNNNNRTG 8 cut(s) 394, 443, 1960, 3212, 3254, 3476, 3633, 3896
Msp20I TGGCCA 1 cut(s) 1515
MspCI CTTAAG 1 cut(s) 3461
MspI CCGG 5 cut(s) 656, 1268, 2279, 2976, 4056
MspR9I CCNGG 8 cut(s) 1269, 2219, 2834, 2977, 2998, 3374, 4013, 4409
MunI CAATTG 2 cut(s) 798, 2802
Mva1269I GAATGC 1 cut(s) 3194
MvaI CCWGG 6 cut(s) 2219, 2834, 2998, 3374, 4013, 4409
MvnI CGCG 2 cut(s) 1618, 3696
NciI CCSGG 2 cut(s) 1269, 2977
NdeI CATATG 1 cut(s) 2651
NheI GCTAGC 1 cut(s) 202
NmuCI GTSAC 5 cut(s) 702, 1808, 2965, 3485, 3712
NsiI ATGCAT 2 cut(s) 2754, 3440
NspI RCATGY 6 cut(s) 1809, 1959, 2554, 3259, 3720, 3935
OliI CACNNNNGTG 2 cut(s) 3476, 3896
PaeI GCATGC 1 cut(s) 2554
PagI TCATGA 3 cut(s) 589, 2302, 4105
PceI AGGCCT 1 cut(s) 3305
PciI ACATGT 2 cut(s) 1805, 3931
PctI GAATGC 1 cut(s) 3194
PdmI GAANNNNTTC 6 cut(s) 576, 2084, 2619, 2909, 3804, 4306
PfoI TCCNGGA 1 cut(s) 4011
PleI GAGTC 8 cut(s) 353, 640, 709, 811, 1119, 1298, 2021, 2195
PpsI GAGTC 8 cut(s) 353, 640, 709, 811, 1119, 1298, 2021, 2195
PscI ACATGT 2 cut(s) 1805, 3931
PshBI ATTAAT 1 cut(s) 2448
Psp124BI GAGCTC 1 cut(s) 527
Psp6I CCWGG 6 cut(s) 2217, 2832, 2996, 3372, 4011, 4407
PspFI CCCAGC 2 cut(s) 3014, 4151
PspGI CCWGG 6 cut(s) 2217, 2832, 2996, 3372, 4011, 4407
PspPI GGNCC 6 cut(s) 28, 866, 2377, 2523, 2893, 3922
PstI CTGCAG 2 cut(s) 1303, 1918
PsuI RGATCY 5 cut(s) 155, 988, 2441, 2709, 3164
RseI CAYNNNNRTG 8 cut(s) 394, 443, 1960, 3212, 3254, 3476, 3633, 3896
SacI GAGCTC 1 cut(s) 527
Sau96I GGNCC 6 cut(s) 28, 866, 2377, 2523, 2893, 3922
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 8 cut(s) 354, 641, 710, 811, 1120, 1298, 2021, 2195
ScrFI CCNGG 8 cut(s) 1269, 2219, 2834, 2977, 2998, 3374, 4013, 4409
SduI GDGCHC 6 cut(s) 376, 527, 3298, 3473, 3702, 4420
SfcI CTRYAG 5 cut(s) 13, 1299, 1351, 1914, 2874
SinI GGWCC 5 cut(s) 28, 866, 2377, 2523, 3922
SmiMI CAYNNNNRTG 8 cut(s) 394, 443, 1960, 3212, 3254, 3476, 3633, 3896
SmlI CTYRAG 9 cut(s) 905, 1713, 1908, 2405, 3005, 3194, 3461, 3885, 4097
SmoI CTYRAG 9 cut(s) 905, 1713, 1908, 2405, 3005, 3194, 3461, 3885, 4097
SphI GCATGC 1 cut(s) 2554
SseBI AGGCCT 1 cut(s) 3305
SspI AATATT 1 cut(s) 2160
SspMI CTAG 8 cut(s) 203, 2003, 2243, 2489, 3419, 3563, 3962, 4421
SstI GAGCTC 1 cut(s) 527
StuI AGGCCT 1 cut(s) 3305
StyD4I CCNGG 8 cut(s) 1267, 2217, 2832, 2975, 2996, 3372, 4011, 4407
StyI CCWWGG 2 cut(s) 2843, 4350
TaaI ACNGT 9 cut(s) 64, 126, 454, 1095, 1218, 2077, 2682, 3475, 3755
TaiI ACGT 3 cut(s) 933, 2965, 3528
TaqI TCGA 6 cut(s) 246, 842, 1114, 1394, 2613, 3341
TatI WGTACW 8 cut(s) 735, 1090, 1102, 1117, 1964, 2744, 3142, 3265
TauI GCSGC 4 cut(s) 840, 1621, 4329, 4358
TseFI GTSAC 5 cut(s) 702, 1808, 2965, 3485, 3712
Tsp45I GTSAC 5 cut(s) 702, 1808, 2965, 3485, 3712
TspGWI ACGGA 2 cut(s) 487, 4383
Vha464I CTTAAG 1 cut(s) 3461
VpaK11BI GGWCC 5 cut(s) 28, 866, 2377, 2523, 3922
VspI ATTAAT 1 cut(s) 2448
XagI CCTNNNNNAGG 1 cut(s) 3276
XapI RAATTY 4 cut(s) 322, 967, 3317, 3804
XceI RCATGY 6 cut(s) 1809, 1959, 2554, 3259, 3720, 3935
XmiI GTMKAC 2 cut(s) 2199, 2479
XmnI GAANNNNTTC 6 cut(s) 576, 2084, 2619, 2909, 3804, 4306
XspI CTAG 8 cut(s) 203, 2003, 2243, 2489, 3419, 3563, 3962, 4421
ZrmI AGTACT 1 cut(s) 737
Zsp2I ATGCAT 2 cut(s) 2754, 3440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.