Rroxscaffold_2G00150380

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001838
Physical Location & Seq
Forward (+)
87785269 .. 87793092
7824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00150380.1

Sequence Viewer

Length: 4029 bp
ATGACCACAGCCATGACCACTACTACTACTAGTTCATCACAGATTCTATGCCACACGGCGGCTTTCGTCTCTGAAACACTTTCGCAATCGGACCTCCGCCGCCACCTCTTCTCCACGCTTCGCCGCAGGCTCCCTCCACCATTGGGTTCCAGCCAAACCGCGGCTATTAAATCCCTCAACCTCGCCGCGGAAACCCTAGAAAACGCCATCGCCGAATCCAGCGCAGCCGTCCGGACCTCCTCCCTCGGCCTCTGTGAGAAGCTCCTCCTCTCCGGGCCCAAAAGCCCCTTCTCCTCGTTCCTCCTCTCCCTCATCTACGGCCTCTGCCGCCGCTCGATCGATGCCGCCGTCTGCATCCTCGAGATCTTCCTATCCGAGCCGGCGCTCGCCCGGACGGAATTGGCTCCGGTGCTGTTCGAGGAGCTGTTCCTCATCCACCTCCTCCCGGTTCTCCAGTGGTACAATCGCCGGCGATCGCAGATTCTGCCGTCGCTGTCCCCAAATGTCAGCTACGAAAACGACGACGACGACGTTTCGATCAACGACATGTCGGTGGCGTTTCCGGCGGGGTCGAATTCGCTGTCGAAGATGAGCGGGAACCAGACGGCGGGGCTGAAGGAGCTGGAGAGCAGATACGAGAGGGTTTTGGATGAGAATTGTAGGGTTTTCAGTGAGTATTTCAGGGAGGTTTTGGAGAATAAAGATGGAGGAAGAGTGATTGATCCACCGGCTGTGGTGTTGGAATTGAGTGAAGGAAGTGATGAGTTCCGGTATGATGATCGGAGCACCGGAGAAGATGAAGCCGGATTTGAAAACGGACGGTACAATCCAATTTGGGCTGAAGCAGAAAATTCAGTTGAATCGTTCAGCAGGGGCAGCAGTGTAAGAAGAAGACGCAAGTCTCCCTCATTTTATCCTCAAAGAGTTGCACTTGATAATATCATCACTGGCCACAAACCCTCATGGAGTTCGGGAGCAACGCCAAATGTAAATTCAGATTCTGAGCTGGAAGAATCTTCTTTGGAGGGCCATTCTCTTGATTCTTCTTTGGATTCCGAAGCTGAAATTGAGGAAAACAACACAAGACTGGAATTATTTGATTCTGGGCGGAACCAAATCCAAAAGCTAAAGCAACCCATTTCCGCAGAATCAAGTCGTTCATCTCCAAATCCGCTCATGGCAGATTCTGAAAACCAATCTGGTGGTGCAAAATTTACACCTCCTAAGGACTTTGTGTGTCCCATAACTAGTACCCTTTTTGATGATCCAGTGACACTTGAGACTGGTCAGACATATGAGCGAAAAGCCATCCAAGAATGGCTTGATAGAGGAAACTCAACCTGCCCAATTACCCGCCAGAATTTGCAAAGCACCCAACTGCCTAAGACCAACTATGTCCTCAAACGTCTAATTGCAAGTTGGCAAGAACAGAACCCTGGTATAGCCTTCAGCCCATCTGATAATCCAAGCCCGCAGATTGAGCCAATGGTGAAACCCATAATGCCTTCAACTTCTCCTGTCAGTGTCATAAGCCAAGCTGCTATTGATAGTGCAGTCAGTGAGTTAAGAATTGCCATTACCAATCTTTGTATGTCAGAAATTCTCAAGGAGTCTGAAATGGCTGTTCTTCGTATTGAGCGTTTCTGGCAGGAAGCAAATGTGGAATGGGATATTCAGAATATGCTCTCAAAACCTCCGGTTATTAATGGTTTCGTGGAGGTTCTTTTCAATTCGGTCGATCCTAGAGTTCTAACAGCCACAGTTTTCCTCTTGTCTGAGCTGGGTTCAAGAGACAATGCTGTGATTCAGACACTAACCCAGGTTGACTCTGATGTGGAATGTATTGTGGCTCTTTTCAAAAAGGGGTTGATGGAGGCTGTTGTCTTGATATATCTGCTAAGGCATTGCATTCTTAATCTAGTAGAGATGGACCTGGTGGACTCTCTCTTAATGGTTATTAGAAAGAGAGATGATGATTGGGCTAATATGTGTCTAAAGCCTAAATCAGCTGCAGTGGTTTTACTGGGGCATATTATTGGTGGCAGTGAATCAATCGCCAATGCTGTGACTTCTGAAAAAGCACTTGAAAGAATAATCAGCAGTTTGGAATCTGAATCAGCAGAAGAGAAGACTGCAGCGATTGGGATCTTATTAAGATGTATGCAACAGGATGGGCACTGCAGGAACACAATAGCTGATAAAGCTGAGTTGGCTCCTGTTTTGGATAGCTTCACGGGTGCAAAGGATGAAGAACGGTTTGAGATAGTTCTCTTTTTGTCCGAGTTAGTGAAATTAAACAGGAGGACATTCAATGAACAAATCCTGCATATCATAAAGGATGAGGGCCCTTTCAGTACAATGCACACCCTCCTTATTTATCTACAGACTGCTGCTCAAGACCATTGTCCTGTTGTGGCCAGTCTACTACTTCAACTTGATCTTCTGGCAGAGCCGAGAAAGATGAGCATATACCGTGAAGAGGCAATGGACACTCTCATCTCATGTCTCAGAAATATTGAGGTCCCTGTTGCCCAAATCGCTGCTGCTGAGACCATTATGTCACTGCAAGGGACGTTCACCACATCCGGAAAGCCTCTTGCTAGGGCCGTCCTTCTAAAACGTGCTGGCCTTGACAAAATTTATAAACAACGTGTGCGAATGGATCAACTTAGCAACTTTTCTGGAGAAGATGAAACTGTTGAGGAAGAAAAGGCGGCTGATAACTGGGAAAGAAAAATGGCATTTGTTTTAGCAAGCCACGAGTTTGGTCTACTTTTTGAGGCCTTGGCAGAAGGATTGAAGAGCAGAATCTCAGAATTATGTTCTGCATGCTTTGTGTCAGCCACATGGCTCGTACACATGCTCAATGCCCTTCCAGATACAGGAATACGAGGAGCAGCTCGGCTGTGCTTGATTAAACGGTTCATATCAATATTCAAGTCTGCCAAGGATACTGAAGACAAAGCACTTGCTTTGCTGGCGTTGGGCAGCTTCATCCGTGATCCTGAGGGGATGCAAGAATTAAATTCTTGCAGGAGGGATATCGTAAAAGGCCTGAGAGAGCTTAAGAGATACACTCCCTTGGCATTTCAAATGCTAAAAGTCTTTTCTGATGGGCAAGACTCTAGTGCCGAATTGTGGGATCATAAAGAATTAGTTCAAGTAGACTGCAGTGAGAACGGAGAGGTTCTTTCAGTAGTTTGTTTCAAGGATAAAATATTTTCTGGCCATTCAGATGGAACTATAAAGGTGTGGACGGGTAAAGGTAGCATTCTCCATCTAATCCAGGAAATTCGAGAACATACAAAGGCAGTTACTAGCTTGGCAATTTTGCAATCAAAGGAGATTCTATACAGTGGCTCACTAGATAGAACAACAAGGGTCTGGTCTATCAGCAATGAAGGAATCCAGTGTGTAGAAGTCCATGATATGAAGGATCAAGTTCATAATCTGGCCGTCACCAATAGTTTTTCATGCTTCATTCCACAAAGCAATGGTATCAAGGCTCACTCATGGAGCGGAGGATCCAAAATGTTAAATTCCAGCAAATCTGTAAAGTGCTTTGCTCTTGTAAATGGGAAAATATATTGTGGATGCCATGATAGCAGTGTTCAGGAGATTGATTTGGTCACAGGAACACTCAGTACCATTCAAACCGGCTCCCGGAAATTACTTGGGAAATCAAATCCTATCCATTGCATTCAGTATCACAGTGGAATGATATATGCAGCAGGTTCTTCGTTGGATGGAGCAGCAGTGAAGATTTGGAGTGCTGCAAACTTTAGTTTGGTTGGGTCTCTGTCTACTACACTAGAAGTGCGGGCTATGGCAATAAGCTCGGAACTGATCTATTTGGGAGGTAAAGGAGGATGTGTGGAAATTTGGGATAGGGAGAAGCAGAACAAAATAATAGACACACTACAAACTGGCACAAACTGTAAGGTTCTTTCCTTGGCTATTGATCCCAATGAGGAAGTTTTGGTCACTGGAACATCTGATGGCCGAATCCAGGCATGGGGACTGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1342

Amino Acids

148.09

Weight (kDa)

5.24

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 20 - 235 2.8e-19 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 408 - 478 5.1e-17 U-box domain
ARM_LIN_2nd PF23654 518 - 675 9.6e-60 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 684 - 1037 4.4e-144 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
WD40_Prp19 PF24814 1034 - 1146 1.7e-08 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1037 - 1142 1.7e-07 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 1041 - 1148 1.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1046 - 1164 6.3e-09 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 1062 - 1156 2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 1065 - 1135 7.1e-07 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 1070 - 1236 7.2e-09 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 1070 - 1155 3.9e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 1071 - 1152 8.8e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 1162 - 1340 2e-07 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 1182 - 1340 8.2e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1193 - 1339 1.3e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1197 - 1336 8.2e-08 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2643
AasI GACNNNNNNGTC 1 cut(s) 2557
Acc36I ACCTGC 2 cut(s) 1349, 3722
AccBSI CCGCTC 4 cut(s) 333, 594, 1174, 3519
AccI GTMKAC 4 cut(s) 2422, 2770, 3165, 3803
AccII CGCG 2 cut(s) 161, 188
AccIII TCCGGA 2 cut(s) 231, 2585
AcoI YGGCCR 5 cut(s) 949, 2415, 3226, 3453, 4000
AcuI CTGAAG 4 cut(s) 635, 861, 1432, 2976
AfaI GTAC 6 cut(s) 461, 824, 1252, 2356, 2856, 3646
AflII CTTAAG 1 cut(s) 3065
AflIII ACRYGT 2 cut(s) 546, 2650
AhlI ACTAGT 2 cut(s) 29, 1247
AjnI CCWGG 5 cut(s) 1435, 1818, 1932, 3285, 4008
AjuI GAANNNNNNNTTGG 4 cut(s) 272, 304, 976, 1008
Alw21I GWGCWC 1 cut(s) 788
Alw26I GTCTC 7 cut(s) 73, 906, 1274, 1785, 2510, 2543, 3801
AlwNI CAGNNNCTG 3 cut(s) 484, 1001, 1187
Ama87I CYCGRG 1 cut(s) 359
Aor13HI TCCGGA 2 cut(s) 231, 2585
ApaI GGGCCC 2 cut(s) 279, 2348
ArsI GACNNNNNNTTYG 2 cut(s) 566, 598
AseI ATTAAT 1 cut(s) 1704
AsiSI GCGATCGC 1 cut(s) 476
Asp700I GAANNNNTTC 1 cut(s) 3156
AspLEI GCGC 2 cut(s) 224, 385
AsuC2I CCSGG 4 cut(s) 274, 391, 446, 3664
AsuHPI GGTGA 3 cut(s) 1501, 2569, 3451
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 4 cut(s) 91, 234, 1930, 2521
AxyI CCTNAGG 2 cut(s) 1224, 3006
BaeGI GKGCMC 3 cut(s) 279, 2178, 2348
BalI TGGCCA 3 cut(s) 951, 2417, 3228
BamHI GGATCC 1 cut(s) 3524
BanII GRGCYC 2 cut(s) 279, 2348
BauI CACGAG 1 cut(s) 2759
BbsI GAAGAC 3 cut(s) 898, 2135, 2964
Bbv12I GWGCWC 1 cut(s) 788
BceAI ACGGC 8 cut(s) 72, 212, 332, 334, 472, 621, 2591, 3440
BcgI CGANNNNNNTGC 4 cut(s) 3720, 3754, 3819, 3853
BciT130I CCWGG 5 cut(s) 1437, 1820, 1934, 3287, 4010
BciVI GTATCC 1 cut(s) 2944
BcnI CCSGG 4 cut(s) 274, 391, 446, 3664
BcoDI GTCTC 7 cut(s) 73, 906, 1274, 1785, 2510, 2543, 3801
BcuI ACTAGT 2 cut(s) 29, 1247
BfmI CTRYAG 5 cut(s) 2010, 2133, 2179, 2381, 3169
BfoI RGCGCY 1 cut(s) 386
BfrI CTTAAG 1 cut(s) 3065
BfuAI ACCTGC 2 cut(s) 1349, 3722
BfuI GTATCC 1 cut(s) 2944
BglII AGATCT 1 cut(s) 363
Bme1390I CCNGG 9 cut(s) 274, 391, 446, 1437, 1820, 1934, 3287, 3664, 4010
Bme18I GGWCC 4 cut(s) 91, 234, 1930, 2521
BmeT110I CYCGRG 1 cut(s) 359
BmrFI CCNGG 9 cut(s) 274, 391, 446, 1437, 1820, 1934, 3287, 3664, 4010
BmrI ACTGGG 2 cut(s) 2033, 2734
BmsI GCATC 4 cut(s) 331, 363, 3003, 3584
BmuI ACTGGG 2 cut(s) 2033, 2734
BoxI GACNNNNGTC 2 cut(s) 898, 2403
BpiI GAAGAC 3 cut(s) 898, 2135, 2964
BplI GAGNNNNNCTC 2 cut(s) 3061, 3093
BpmI CTGGAG 3 cut(s) 437, 644, 2703
Bpu10I CCTNAGC 1 cut(s) 1898
BpuEI CTTGAG 3 cut(s) 1298, 1589, 2379
BpuMI CCSGG 4 cut(s) 274, 391, 446, 3664
Bsa29I ATCGAT 1 cut(s) 339
BsaBI GATNNNNATC 1 cut(s) 2144
BsaI GGTCTC 2 cut(s) 2543, 3801
BsaJI CCNNGG 9 cut(s) 159, 186, 244, 1435, 1818, 2784, 2946, 3081, 3951
BsaWI WCCGGW 6 cut(s) 231, 406, 768, 788, 1696, 2585
BsaXI ACNNNNNCTCC 8 cut(s) 95, 125, 699, 729, 3741, 3771, 3858, 3888
Bse118I RCCGGY 4 cut(s) 379, 468, 727, 3656
Bse21I CCTNAGG 2 cut(s) 1224, 3006
Bse3DI GCAATG 5 cut(s) 1903, 2490, 3403, 3499, 3694
Bse8I GATNNNNATC 1 cut(s) 2144
BseAI TCCGGA 2 cut(s) 231, 2585
BseBI CCWGG 5 cut(s) 1437, 1820, 1934, 3287, 4010
BseCI ATCGAT 1 cut(s) 339
BseDI CCNNGG 9 cut(s) 159, 186, 244, 1435, 1818, 2784, 2946, 3081, 3951
BseJI GATNNNNATC 1 cut(s) 2144
BseMI GCAATG 5 cut(s) 1903, 2490, 3403, 3499, 3694
BseRI GAGGAG 8 cut(s) 229, 254, 257, 283, 293, 431, 434, 2907
BseSI GKGCMC 3 cut(s) 279, 2178, 2348
BseYI CCCAGC 1 cut(s) 1780
BsgI GTGCAG 1 cut(s) 1572
Bsh1236I CGCG 2 cut(s) 161, 188
Bsh1285I CGRYCG 3 cut(s) 339, 476, 1737
BshVI ATCGAT 1 cut(s) 339
BsiEI CGRYCG 3 cut(s) 339, 476, 1737
BsiHKAI GWGCWC 1 cut(s) 788
BsiHKCI CYCGRG 1 cut(s) 359
BslFI GGGAC 4 cut(s) 481, 1224, 2507, 2584
BsmAI GTCTC 7 cut(s) 73, 906, 1274, 1785, 2510, 2543, 3801
BsmBI CGTCTC 1 cut(s) 73
BsmFI GGGAC 4 cut(s) 481, 1224, 2507, 2584
BsmI GAATGC 3 cut(s) 1908, 3270, 3699
Bso31I GGTCTC 2 cut(s) 2543, 3801
BsoBI CYCGRG 1 cut(s) 359
Bsp120I GGGCCC 2 cut(s) 275, 2344
Bsp1286I GDGCHC 4 cut(s) 279, 788, 2178, 2348
Bsp13I TCCGGA 2 cut(s) 231, 2585
BspDI ATCGAT 1 cut(s) 339
BspEI TCCGGA 2 cut(s) 231, 2585
BspFNI CGCG 2 cut(s) 161, 188
BspMAI CTGCAG 4 cut(s) 2014, 2137, 2183, 3173
BspMI ACCTGC 2 cut(s) 1349, 3722
BspQI GCTCTTC 1 cut(s) 2795
BspTI CTTAAG 1 cut(s) 3065
BspTNI GGTCTC 2 cut(s) 2543, 3801
BsrBI CCGCTC 4 cut(s) 333, 594, 1174, 3519
BsrDI GCAATG 5 cut(s) 1903, 2490, 3403, 3499, 3694
BsrFI RCCGGY 4 cut(s) 379, 468, 727, 3656
BssAI RCCGGY 4 cut(s) 379, 468, 727, 3656
BssECI CCNNGG 9 cut(s) 159, 186, 244, 1435, 1818, 2784, 2946, 3081, 3951
BssSI CACGAG 1 cut(s) 2759
BssT1I CCWWGG 4 cut(s) 2784, 2946, 3081, 3951
Bst2BI CACGAG 1 cut(s) 2759
Bst2UI CCWGG 5 cut(s) 1437, 1820, 1934, 3287, 4010
Bst4CI ACNGT 9 cut(s) 822, 1762, 2256, 2474, 2698, 2922, 3356, 3713, 3938
Bst6I CTCTTC 5 cut(s) 113, 706, 2118, 2472, 2795
BstAFI CTTAAG 1 cut(s) 3065
BstAPI GCANNNNNTGC 1 cut(s) 484
BstDSI CCRYGG 2 cut(s) 159, 186
BstFNI CGCG 2 cut(s) 161, 188
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 2 cut(s) 224, 385
BstMAI GTCTC 7 cut(s) 73, 906, 1274, 1785, 2510, 2543, 3801
BstMCI CGRYCG 3 cut(s) 339, 476, 1737
BstNI CCWGG 5 cut(s) 1437, 1820, 1934, 3287, 4010
BstNSI RCATGY 3 cut(s) 550, 2832, 2863
BstPAI GACNNNNGTC 2 cut(s) 898, 2403
BstSCI CCNGG 9 cut(s) 272, 389, 444, 1435, 1818, 1932, 3285, 3662, 4008
BstSFI CTRYAG 5 cut(s) 2010, 2133, 2179, 2381, 3169
BstSLI GKGCMC 3 cut(s) 279, 2178, 2348
BstUI CGCG 2 cut(s) 161, 188
BstV2I GAAGAC 3 cut(s) 898, 2135, 2964
BstX2I RGATCY 3 cut(s) 363, 2145, 3524
BstXI CCANNNNNNTGG 3 cut(s) 1202, 2765, 3236
BstYI RGATCY 3 cut(s) 363, 2145, 3524
Bsu15I ATCGAT 1 cut(s) 339
Bsu36I CCTNAGG 2 cut(s) 1224, 3006
BsuI GTATCC 1 cut(s) 2944
BsuTUI ATCGAT 1 cut(s) 339
BtgI CCRYGG 2 cut(s) 159, 186
BtgZI GCGATG 1 cut(s) 193
BtsI GCAGTG 8 cut(s) 886, 2019, 2050, 2176, 2561, 3178, 3613, 3762
BveI ACCTGC 2 cut(s) 1349, 3722
CaiI CAGNNNCTG 3 cut(s) 484, 1001, 1187
CfoI GCGC 2 cut(s) 224, 385
Cfr10I RCCGGY 4 cut(s) 379, 468, 727, 3656
Cfr42I CCGCGG 2 cut(s) 162, 189
ClaI ATCGAT 1 cut(s) 339
CseI GACGC 1 cut(s) 903
CsiI ACCWGGT 1 cut(s) 1932
Csp6I GTAC 6 cut(s) 460, 823, 1251, 2355, 2855, 3645
CviQI GTAC 6 cut(s) 460, 823, 1251, 2355, 2855, 3645
DrdI GACNNNNNNGTC 1 cut(s) 2557
DseDI GACNNNNNNGTC 1 cut(s) 2557
EaeI YGGCCR 5 cut(s) 949, 2415, 3226, 3453, 4000
Eam1104I CTCTTC 5 cut(s) 113, 706, 2118, 2472, 2795
EarI CTCTTC 5 cut(s) 113, 706, 2118, 2472, 2795
EciI GGCGGA 2 cut(s) 86, 1123
Eco130I CCWWGG 4 cut(s) 2784, 2946, 3081, 3951
Eco147I AGGCCT 2 cut(s) 2783, 3054
Eco24I GRGCYC 2 cut(s) 279, 2348
Eco31I GGTCTC 2 cut(s) 2543, 3801
Eco32I GATATC 1 cut(s) 3043
Eco47I GGWCC 4 cut(s) 91, 234, 1930, 2521
Eco57I CTGAAG 4 cut(s) 635, 861, 1432, 2976
Eco81I CCTNAGG 2 cut(s) 1224, 3006
Eco88I CYCGRG 1 cut(s) 359
EcoO109I RGGNCCY 3 cut(s) 2344, 2345, 2521
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 5 cut(s) 1435, 1818, 1932, 3285, 4008
EcoRV GATATC 1 cut(s) 3043
EcoT14I CCWWGG 4 cut(s) 2784, 2946, 3081, 3951
EcoT38I GRGCYC 2 cut(s) 279, 2348
ErhI CCWWGG 4 cut(s) 2784, 2946, 3081, 3951
Esp3I CGTCTC 1 cut(s) 73
FalI AAGNNNNNCTT 2 cut(s) 1305, 1337
FaqI GGGAC 4 cut(s) 481, 1224, 2507, 2584
FauI CCCGC 6 cut(s) 559, 587, 601, 1361, 1479, 3813
FauNDI CATATG 1 cut(s) 1294
FblI GTMKAC 4 cut(s) 2422, 2770, 3165, 3803
FriOI GRGCYC 2 cut(s) 279, 2348
GlaI GCGC 2 cut(s) 223, 384
GsaI CCCAGC 1 cut(s) 1784
GsuI CTGGAG 3 cut(s) 437, 644, 2703
HaeII RGCGCY 1 cut(s) 386
HgaI GACGC 1 cut(s) 903
HhaI GCGC 2 cut(s) 224, 385
Hin6I GCGC 2 cut(s) 222, 383
HinP1I GCGC 2 cut(s) 222, 383
HincII GTYRAC 1 cut(s) 1825
HindII GTYRAC 1 cut(s) 1825
HphI GGTGA 3 cut(s) 1501, 2569, 3451
Hpy166II GTNNAC 9 cut(s) 1825, 1939, 2423, 2577, 2771, 2857, 3166, 3255, 3804
Hpy8I GTNNAC 9 cut(s) 1825, 1939, 2423, 2577, 2771, 2857, 3166, 3255, 3804
Hpy99I CGWCG 5 cut(s) 493, 524, 527, 530, 533
HpyCH4III ACNGT 9 cut(s) 822, 1762, 2256, 2474, 2698, 2922, 3356, 3713, 3938
HpyCH4IV ACGT 5 cut(s) 531, 1405, 2573, 2620, 2650
HpySE526I ACGT 5 cut(s) 531, 1405, 2573, 2620, 2650
HspAI GCGC 2 cut(s) 222, 383
Kpn2I TCCGGA 2 cut(s) 231, 2585
KroI GCCGGC 2 cut(s) 379, 468
KroNI GCCGGC 2 cut(s) 381, 470
KspI CCGCGG 2 cut(s) 162, 189
LguI GCTCTTC 1 cut(s) 2795
LweI GCATC 4 cut(s) 331, 363, 3003, 3584
MabI ACCWGGT 1 cut(s) 1932
MaeII ACGT 5 cut(s) 531, 1405, 2573, 2620, 2650
MaeIII GTNAC 7 cut(s) 1270, 2065, 2559, 3313, 3457, 3628, 3982
MbiI CCGCTC 4 cut(s) 333, 594, 1174, 3519
MflI RGATCY 3 cut(s) 363, 2145, 3524
MhlI GDGCHC 4 cut(s) 279, 788, 2178, 2348
MlsI TGGCCA 3 cut(s) 951, 2417, 3228
MluNI TGGCCA 3 cut(s) 951, 2417, 3228
MlyI GAGTC 4 cut(s) 1619, 1820, 1934, 3116
MmeI TCCRAC 2 cut(s) 720, 3723
Mox20I TGGCCA 3 cut(s) 951, 2417, 3228
MreI CGCCGGCG 1 cut(s) 468
MroI TCCGGA 2 cut(s) 231, 2585
MroNI GCCGGC 2 cut(s) 379, 468
MroXI GAANNNNTTC 1 cut(s) 3156
MscI TGGCCA 3 cut(s) 951, 2417, 3228
MslI CAYNNNNRTG 3 cut(s) 11, 551, 3234
Msp20I TGGCCA 3 cut(s) 951, 2417, 3228
MspA1I CMGCKG 3 cut(s) 161, 188, 2009
MspCI CTTAAG 1 cut(s) 3065
MspR9I CCNGG 9 cut(s) 274, 391, 446, 1437, 1820, 1934, 3287, 3664, 4010
Mva1269I GAATGC 3 cut(s) 1908, 3270, 3699
MvaI CCWGG 5 cut(s) 1437, 1820, 1934, 3287, 4010
MvnI CGCG 2 cut(s) 161, 188
NaeI GCCGGC 2 cut(s) 381, 470
NciI CCSGG 4 cut(s) 274, 391, 446, 3664
NdeI CATATG 1 cut(s) 1294
NgoMIV GCCGGC 2 cut(s) 379, 468
NmeAIII GCCGAG 3 cut(s) 225, 2478, 2881
NmuCI GTSAC 6 cut(s) 1270, 2065, 2559, 3457, 3628, 3982
NspI RCATGY 3 cut(s) 550, 2832, 2863
PaeI GCATGC 1 cut(s) 2832
PaeR7I CTCGAG 1 cut(s) 359
PceI AGGCCT 2 cut(s) 2783, 3054
PciI ACATGT 1 cut(s) 546
PciSI GCTCTTC 1 cut(s) 2795
PcsI WCGNNNNNNNCGW 6 cut(s) 210, 345, 519, 525, 528, 1636
PctI GAATGC 3 cut(s) 1908, 3270, 3699
PdiI GCCGGC 2 cut(s) 381, 470
PdmI GAANNNNTTC 1 cut(s) 3156
PfoI TCCNGGA 2 cut(s) 3285, 3662
Ple19I CGATCG 2 cut(s) 339, 476
PleI GAGTC 4 cut(s) 1618, 1820, 1934, 3116
PpsI GAGTC 4 cut(s) 1618, 1820, 1934, 3116
PpuMI RGGWCCY 1 cut(s) 2521
PscI ACATGT 1 cut(s) 546
PshAI GACNNNNGTC 2 cut(s) 898, 2403
PshBI ATTAAT 1 cut(s) 1704
PsiI TTATAA 1 cut(s) 2643
Psp5II RGGWCCY 1 cut(s) 2521
Psp6I CCWGG 5 cut(s) 1435, 1818, 1932, 3285, 4008
PspFI CCCAGC 1 cut(s) 1780
PspGI CCWGG 5 cut(s) 1435, 1818, 1932, 3285, 4008
PspOMI GGGCCC 2 cut(s) 275, 2344
PspPPI RGGWCCY 1 cut(s) 2521
PsrI GAACNNNNNNTAC 2 cut(s) 3628, 3660
PstI CTGCAG 4 cut(s) 2014, 2137, 2183, 3173
PstNI CAGNNNCTG 3 cut(s) 484, 1001, 1187
PsuI RGATCY 3 cut(s) 363, 2145, 3524
PvuI CGATCG 2 cut(s) 339, 476
PvuII CAGCTG 1 cut(s) 2009
RgaI GCGATCGC 1 cut(s) 476
RsaI GTAC 6 cut(s) 461, 824, 1252, 2356, 2856, 3646
RsaNI GTAC 6 cut(s) 460, 823, 1251, 2355, 2855, 3645
RseI CAYNNNNRTG 3 cut(s) 11, 551, 3234
SacII CCGCGG 2 cut(s) 162, 189
SapI GCTCTTC 1 cut(s) 2795
SchI GAGTC 4 cut(s) 1619, 1820, 1934, 3116
ScrFI CCNGG 9 cut(s) 274, 391, 446, 1437, 1820, 1934, 3287, 3664, 4010
SduI GDGCHC 4 cut(s) 279, 788, 2178, 2348
SexAI ACCWGGT 1 cut(s) 1932
SfaAI GCGATCGC 1 cut(s) 476
SfaNI GCATC 4 cut(s) 331, 363, 3003, 3584
SfcI CTRYAG 5 cut(s) 2010, 2133, 2179, 2381, 3169
Sfr274I CTCGAG 1 cut(s) 359
Sfr303I CCGCGG 2 cut(s) 162, 189
SgfI GCGATCGC 1 cut(s) 476
SgrAI CRCCGGYG 1 cut(s) 468
SgrBI CCGCGG 2 cut(s) 162, 189
SinI GGWCC 4 cut(s) 91, 234, 1930, 2521
SlaI CTCGAG 1 cut(s) 359
SmiMI CAYNNNNRTG 3 cut(s) 11, 551, 3234
SmlI CTYRAG 5 cut(s) 359, 1277, 1604, 2394, 3065
SmoI CTYRAG 5 cut(s) 359, 1277, 1604, 2394, 3065
SpeI ACTAGT 2 cut(s) 29, 1247
SphI GCATGC 1 cut(s) 2832
SseBI AGGCCT 2 cut(s) 2783, 3054
SspI AATATT 3 cut(s) 2515, 2934, 3219
StuI AGGCCT 2 cut(s) 2783, 3054
StyD4I CCNGG 9 cut(s) 272, 389, 444, 1435, 1818, 1932, 3285, 3662, 4008
StyI CCWWGG 4 cut(s) 2784, 2946, 3081, 3951
TaaI ACNGT 9 cut(s) 822, 1762, 2256, 2474, 2698, 2922, 3356, 3713, 3938
TaiI ACGT 5 cut(s) 534, 1408, 2576, 2623, 2653
TaqI TCGA 9 cut(s) 335, 339, 360, 417, 536, 572, 584, 1737, 3295
TaqII GACCGA 1 cut(s) 1723
TatI WGTACW 1 cut(s) 2354
TauI GCSGC 9 cut(s) 62, 102, 126, 164, 188, 330, 333, 347, 2717
TseFI GTSAC 6 cut(s) 1270, 2065, 2559, 3457, 3628, 3982
Tsp45I GTSAC 6 cut(s) 1270, 2065, 2559, 3457, 3628, 3982
TspGWI ACGGA 4 cut(s) 410, 831, 2987, 3195
Vha464I CTTAAG 1 cut(s) 3065
VpaK11BI GGWCC 4 cut(s) 91, 234, 1930, 2521
VspI ATTAAT 1 cut(s) 1704
XceI RCATGY 3 cut(s) 550, 2832, 2863
XhoI CTCGAG 1 cut(s) 359
XmiI GTMKAC 4 cut(s) 2422, 2770, 3165, 3803
XmnI GAANNNNTTC 1 cut(s) 3156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.