Rh6DG431000

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001838
Physical Location & Seq
Reverse (-)
61018107 .. 61023846
5740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG431000.1

Sequence Viewer

Length: 4425 bp
ATGGCTAAGAACTATAGATTTTCAATGGACCAAAAGGACATTGTTAGGGTATTGATTACCACAGTAGATGGTTTTATTCGGGATCAGCTGATCAATAAAGAACAGAGAAGCCAACACAGGGAACAGTGTGCTGAAAGATTGGCAGCTGAAGATGGATCCTGTGGTAAAGAAACCGAGGTTCGTTACTCTGATCAAGCAGTGCTAGCAAATCTGGATTGGGGTATTGAGGCTCTTGAAGAAGCAATCGACACTTCAAACATGGAAACTAAGCTTGCAAGATTAGACCATGCTGAAAAAATGTTGCAAGTTTGTGCCATGCTGAATTCTGATCAGAAAACTGCTGGAGTCCCCAATTTTTACCTCTCAGCTTGGGCACACCTCAACTTAGCATACCTGTGGAAATTGAGGAATAACATCCAAAACTCAGTTCTCCATGTCATTGAGATGTTTACAGTTGATCCTTTTTTCTCACGGATAGATTTTGCTCCTGAAATCTGGAAACTTTTGTTTCTTCCACACATGAGCTCAATTATAGGGTGGTATTCGGAGCAGAGGCACCGGCTTGTGATGGAAGTAATTCCTGATTCTCATGATCTGTCTTTCACAGCTGATCTTGATCAATTTTTCAATGAGTCTTTAATCTTTTCAATGAGGCCGGATCAGATAGAGAAATTGCAAAAGCTGGAGCAGCTTTATGGTGAGTCACTGGATGAGAACACAAGGCTTTATGCCAAGTACTTCAAGGATTGCATGACATCTGATTCAACCTCCAGTAAGAAAGTGATTCCAATGTTGCCAATTGCTGAGCCACCAATGACTCCATTGCATGAGGTCAGCCGCTCGATTCCTGATTTTGTCAAGTTTGGTCCTATCTTGCCCAAGAGTGCTGGCTTTTCTCCCATCCTCAAGTCTAAAGATGGCACAAGAGACGTAAACAGAATGAATCTAACTTCCCCATCTTCACAAAATTTGGAGCCTGTCGGATGGGATCCTCAGGAATGTATCCCCGAGGAGAACGAAGATGAATCTGATTATGAGCCAAATGATGCTAATATGGCTTCTGATCACGAGAAGGAAACGGGAGGAAAAGTACAGTCATCTGTACTCAGGAGTCGAGTACATAGCCCAACAATCTTTTCTCCACTAATTTCTCCTAAAACTTCACCAAAAGTTTCATCTCCAAAACCAGACACAGCTGCTTCTGCATTGCGTTTACTGTCTGTCAAAATCACAGATTCAGCTGTTGCCACCTCTTTGCCTGCATCTCCGGGCATGAGCAATGATTACAGCATCAGCTCTGCAGACTCTGATGTTGAAGTGATAGAGACTACCAAAAGTTGCAGGAAAGTCTATAGCCGAACATCAAGCATGAATAATGAGCTTGTGAAAATGTCGAAAAATAGTCCACCTAATGAAAATGATGAAGGAGGCCAGAGCTGTGTTTCACTTCCATCTTCTGAGAGGATGACTACTAAGTCAAGACCACCTAAGGATTTTGTTTGTCCTATCACTGGCCAGATATTTATTGATCCAGTTACTCTTGAAACAGGTCAGACATACGAAAGAAAAGCAATCCAAGAATGGCTGAAACGAGGAAACACAACCTGTCCCATTACGCGGCAACCCATCTCTACAACTACTCAGCTGCCTAAAACAAACTATGTTTTGAAAAGACTGATAACCTCTTGGAAAGAACAGCATCCTGAACTCGCTCAAGAATGTGCATACTACGAAACACCAAGAAACTCATTCCACCGCTCCTCCGTGAAAGAAGTCCATTCTGGTACCACAACTCCACAAAGAACATGTGACTTCATGGGTCATAGGAATACCGATGATTATATCAGCCAAAGGAGCAAAAGGTTTATGCAGGCAGCTGTTTCTACATCACCAACCAGTGTGATATCTCAAGCTGCAGTTGAGACTATTATCAATGGCTTAAAGCCTCATGTTGCATGTCTGTGTACTTCAGATAAACTTCAAGAGTGTGAAACAGCAGTGTTGGAAATATCCAGATTATGGAAGGACTCAAAGAATCCTGCAATTCATACATTCTTATCGGAGCTGACAACTGTGAATGGATTCATCGAAATACTTTCAGCTTCTCTGAATAGGGAAGTCCTGAGAACATCTATTTACATCCTCTCCGAGCTAATATTTGCCGATGAAAGTGTTGGAGAAACACTTACCAGTGTAGACTCCGACCTTGATTGCCTGGCTGCTCTTTTAAAGAATGGACTAGCTGAGCCTGCTGTTCTCATTTACCAGCTGAGGCCGGTGTTTGCTCAGCTTTCAGCTCATGATCTTATACCCTCCCTTGTGCAGCTAATTCAGAGCAAAAATGAGGAATCAAATGATCTTCAATTAGTAATGGACCCCAAAGATGCTGCATTAGCTATTCTTGAGCAAGTCTTGATGGGAGGAGATGAAAATAGCAGATCCATTAATGCTTTGAGTGTTTTTTCTGCGAGTGGTATACCTGCTCTAGTTAAGTGTTTAGATAGGCCAGAAGGAAGAAGGTCCATTGTTTCTATTCTATTATGCTGCATGCAAGCTGAAAAAACTTGCAGGAACTTGATAGCAAATAGAATTGAGCTGTCTCCTGTTCTCGAACTGTTTCACGCTGGAAGTGATGGTGTGAGAGGCATATGTGTAGAGTTTCTCTCAGAACTGGTTCAGTTGAACAGGAGAACATTATGCAATCAGATCTTGCAGACGATTAAAGACGAAGGAGCATTTAGTACAATGCATACCTTTCTTGTATATCTTCAAATGGCTCCAATGGAGCAGCAACCTGCAATTGCTACTCTCCTTCTTCAGCTTGATCTCCTGGTTGAGCCTTGGAAGATGAGCATTTATAGGGAAGAATCTATAGAGGGACTAATTGAGGCACTCCGAAGGAAAGACTTCTCAAACTCTCAAATGATGGCTTTAGATGCCTTGTTATCTCTTACTGGACGTGTGACTTCATCCGGGGATTCATACACTGAAGCCTGGTTACTCAAGATTGCTGGGTTTGATCAACCTTACAATGCTTTGATGAAGGCAGAGCGGCTAAGAAAACAAGACAGTGATTTGATGGAGACAATGGAAGAAGAAGAGCAAGCAGTAAGCTCTTGGCAGAAAAAAGTTGCTTTTGTACTTTGCAACCATGAGAAGGGATCCATTTTTAAAGCTTTGGAAGAATGCCTCAAGAGTAAATCCATTGAGATGGCAAAGTCATGTCTTGTCATAGCTACATGGCTCACACACATGCTTTCTGTACTTCCTGATACAGGTGTGAAAATCAGTGCTCGCAAGGCCTTGCTTGAAGAATTCATAAATGTTCTTCAATCATCGAATAATTTGGAGGAGAAGATTTTAGCAACCCTGGCTTTGAAATCTTTTGTCAGTGAACCAGCTGCACTTGAAGCACTAGGAGTGTATGCTAAATGCATCTACAAAACATTGAGAAAGCTTAAGAGGAGCACTGTGGTGGCGAGTGACATAATGAAAGCGCTGATGAACTTGTCATCTGTTGACGTAACAGAGTTGTGGAGCTGTGCTGAAGTGGTTGAACTAGATTCAAGCTCAAATGGGGAGGTTACATCTATACTTCATCTAAAAGGCCATGTTTTAAGCAGCCATTCGGATGGAACTATGAAGGTATGGGATGCTGGCAAGAAAGTATTAAGACTGATTCAAGAAGTCCGCGAGCACACAAAAGCTGTCACATGCCTCTATATTTCACCTTCAGGGGATAAATTATACAGCGGTTCGTTAGACAAAACAATTCGGGTGTGGGCAATCAAAGCAGAAGAAATTCATTGCCTTCAGGTTCACGATGTGAAAGAGGTAGTATATGAGCTGGTAGCGAATGCTAAAGTGGCGTGCTTCATTTCTCAAGGCACTGGAGTGAAGGTTTATGAATGGTCTGGGGTCCAAAAACATGTAAACTTCAACAAATATGTAAAGAGCCTAGCCATGTCAGGGAACAGCCTCTATTGTGGTTGCTCTGGTTACAGCATCCAGGAGATCAACTTGGGGAGACATATATCAAGCACATTCTACACCGGCACAAGAAAGCTTTTGGGAAAACAGATGTTCTTTTCCCTTGAGATTCATGATGGCATCCTCTATGCGGGCGGTTCATCAGTTGATGCAACAGCTGGGAAGATATTTTCGCTTCCAAACAAGGCGGTATTAGGATCATTCTCAACTGGATTTGACATCCAACGCATTGCCACCAACAATGACTTGATCTTTACGGCAACAAAGTGTGGGATAATTGAGGTTTGGTTGAAAGAGAGGTTCACAAGAATTGCTTCCATCAAAACAGCTGGCGGCGGTCATGCCAAGATCACATCCTTGGCGGCAGATATGGACGGAGGATTGCTGTTTGCAGGTTCCTCTGATGGCAGAATCCAGGTTTGGGCACTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1474

Amino Acids

163.92

Weight (kDa)

5.75

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 9 - 268 1.9e-75 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 496 - 567 1.6e-15 U-box domain
ARM_LIN_2nd PF23654 642 - 802 3.3e-59 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 803 - 1165 3.3e-115 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR3_1st PF25173 1183 - 1323 2.9e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 1190 - 1267 3.4e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 1195 - 1288 4.8e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 1202 - 1267 2.3e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 1202 - 1303 8.6e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 1203 - 1266 1.4e-08 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 1203 - 1292 7e-09 Prp19 WD40 domain
WD40 PF00400 1226 - 1261 5.3e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1474
Acc36I ACCTGC 3 cut(s) 2488, 2803, 4373
Acc65I GGTACC 1 cut(s) 1784
AccB1I GGYRCC 2 cut(s) 555, 1784
AccB7I CCANNNNNTGG 1 cut(s) 2019
AccBSI CCGCTC 3 cut(s) 840, 1758, 3052
AccI GTMKAC 2 cut(s) 2196, 2476
AccII CGCG 2 cut(s) 1618, 3693
AcoI YGGCCR 1 cut(s) 1513
AcsI RAATTY 4 cut(s) 322, 967, 3314, 3801
AcuI CTGAAG 7 cut(s) 168, 1953, 2801, 3009, 3567, 3717, 3797
AdeI CACNNNGTG 1 cut(s) 3826
AfaI GTAC 9 cut(s) 737, 1092, 1104, 1119, 1786, 1966, 2743, 3141, 3264
AfeI AGCGCT 1 cut(s) 3498
AfiI CCNNNNNNNGG 9 cut(s) 118, 1511, 1617, 2019, 3157, 3275, 3969, 4120, 4411
AflII CTTAAG 1 cut(s) 3458
AflIII ACRYGT 3 cut(s) 1805, 2959, 3928
AjiI CACGTC 1 cut(s) 2960
AjnI CCWGG 6 cut(s) 2214, 2829, 2993, 3369, 4008, 4404
AjuI GAANNNNNNNTTGG 6 cut(s) 411, 443, 1120, 1152, 4393, 4425
AleI CACNNNNGTG 2 cut(s) 3473, 3893
Alw21I GWGCWC 4 cut(s) 527, 3295, 3470, 3699
Alw26I GTCTC 6 cut(s) 921, 1319, 1916, 2604, 3077, 4021
AlwNI CAGNNNCTG 1 cut(s) 1307
Ama87I CYCGRG 1 cut(s) 1007
Aor51HI AGCGCT 1 cut(s) 3498
AoxI GGCC 7 cut(s) 653, 1429, 1513, 2273, 2504, 3300, 3607
ApoI RAATTY 4 cut(s) 322, 967, 3314, 3801
AseI ATTAAT 1 cut(s) 2445
Asp700I GAANNNNTTC 7 cut(s) 576, 2081, 2616, 2673, 2906, 3801, 4303
Asp718I GGTACC 1 cut(s) 1784
AspLEI GCGC 1 cut(s) 3499
AspS9I GGNCC 5 cut(s) 28, 866, 2374, 2520, 3919
AsuC2I CCSGG 2 cut(s) 1269, 2974
AsuHPI GGTGA 4 cut(s) 710, 1155, 1881, 3720
AsuNHI GCTAGC 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 1007
AvaII GGWCC 5 cut(s) 28, 866, 2374, 2520, 3919
AxyI CCTNAGG 2 cut(s) 993, 1488
BaeGI GKGCMC 2 cut(s) 376, 4417
BaeI ACNNNNGTAYC 2 cut(s) 3264, 3297
BalI TGGCCA 1 cut(s) 1515
BamHI GGATCC 3 cut(s) 155, 988, 3161
BanI GGYRCC 2 cut(s) 555, 1784
BanII GRGCYC 1 cut(s) 527
BarI GAAGNNNNNNTAC 2 cut(s) 2982, 3014
BauI CACGAG 1 cut(s) 1067
Bbv12I GWGCWC 4 cut(s) 527, 3295, 3470, 3699
BbvCI CCTCAGC 1 cut(s) 2270
BceAI ACGGC 1 cut(s) 4263
BciT130I CCWGG 6 cut(s) 2216, 2831, 2995, 3371, 4010, 4406
BciVI GTATCC 1 cut(s) 1013
BclI TGATCA 6 cut(s) 90, 190, 328, 616, 1063, 3019
BcnI CCSGG 2 cut(s) 1269, 2974
BcoDI GTCTC 6 cut(s) 921, 1319, 1916, 2604, 3077, 4021
BfaI CTAG 7 cut(s) 203, 2240, 2486, 3416, 3560, 3959, 4418
BfmI CTRYAG 5 cut(s) 13, 1299, 1351, 1914, 2871
BfoI RGCGCY 1 cut(s) 3500
BfrI CTTAAG 1 cut(s) 3458
BfuAI ACCTGC 3 cut(s) 2488, 2803, 4373
BfuI GTATCC 1 cut(s) 1013
BglII AGATCT 1 cut(s) 2706
BlpI GCTNAGC 3 cut(s) 804, 2244, 2286
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 8 cut(s) 1269, 2216, 2831, 2974, 2995, 3371, 4010, 4406
Bme18I GGWCC 5 cut(s) 28, 866, 2374, 2520, 3919
BmeT110I CYCGRG 1 cut(s) 1007
BmgBI CACGTC 1 cut(s) 2960
BmgT120I GGNCC 5 cut(s) 28, 866, 2374, 2520, 3919
BmrFI CCNGG 8 cut(s) 1269, 2216, 2831, 2974, 2995, 3371, 4010, 4406
BmtI GCTAGC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 2648, 2680
BpmI CTGGAG 4 cut(s) 363, 704, 754, 3912
Bpu10I CCTNAGC 1 cut(s) 2270
Bpu1102I GCTNAGC 3 cut(s) 804, 2244, 2286
BpuEI CTTGAG 8 cut(s) 890, 1698, 1893, 2423, 2987, 3176, 3867, 4115
BpuMI CCSGG 2 cut(s) 1269, 2974
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 6 cut(s) 174, 1008, 2840, 2973, 3369, 4347
Bsc4I CCNNNNNNNGG 9 cut(s) 118, 1511, 1617, 2019, 3157, 3275, 3969, 4120, 4411
Bse118I RCCGGY 3 cut(s) 558, 2275, 4052
Bse21I CCTNAGG 2 cut(s) 993, 1488
Bse3DI GCAATG 5 cut(s) 821, 1205, 1285, 3805, 4218
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 6 cut(s) 2216, 2831, 2995, 3371, 4010, 4406
BseDI CCNNGG 6 cut(s) 174, 1008, 2840, 2973, 3369, 4347
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 9 cut(s) 118, 1511, 1617, 2019, 3157, 3275, 3969, 4120, 4411
BseMI GCAATG 5 cut(s) 821, 1205, 1285, 3805, 4218
BseRI GAGGAG 5 cut(s) 1025, 1750, 2436, 3365, 3478
BseSI GKGCMC 2 cut(s) 376, 4417
BseYI CCCAGC 2 cut(s) 3011, 4148
BsgI GTGCAG 2 cut(s) 2342, 3387
Bsh1236I CGCG 2 cut(s) 1618, 3693
BshFI GGCC 7 cut(s) 655, 1431, 1515, 2275, 2506, 3302, 3609
BshNI GGYRCC 2 cut(s) 555, 1784
BsiHKAI GWGCWC 4 cut(s) 527, 3295, 3470, 3699
BsiHKCI CYCGRG 1 cut(s) 1007
BsiSI CCGG 6 cut(s) 559, 656, 1268, 2276, 2973, 4053
BslFI GGGAC 3 cut(s) 332, 1593, 2892
BslI CCNNNNNNNGG 9 cut(s) 118, 1511, 1617, 2019, 3157, 3275, 3969, 4120, 4411
BsmAI GTCTC 6 cut(s) 921, 1319, 1916, 2604, 3077, 4021
BsmBI CGTCTC 1 cut(s) 921
BsmFI GGGAC 3 cut(s) 332, 1593, 2892
BsmI GAATGC 2 cut(s) 3191, 3862
BsnI GGCC 7 cut(s) 655, 1431, 1515, 2275, 2506, 3302, 3609
BsoBI CYCGRG 1 cut(s) 1007
Bsp1286I GDGCHC 6 cut(s) 376, 527, 3295, 3470, 3699, 4417
Bsp1720I GCTNAGC 3 cut(s) 804, 2244, 2286
BspANI GGCC 7 cut(s) 655, 1431, 1515, 2275, 2506, 3302, 3609
BspFNI CGCG 2 cut(s) 1618, 3693
BspHI TCATGA 3 cut(s) 589, 2299, 4102
BspMAI CTGCAG 2 cut(s) 1303, 1918
BspMI ACCTGC 3 cut(s) 2488, 2803, 4373
BspOI GCTAGC 1 cut(s) 206
BspQI GCTCTTC 1 cut(s) 3093
BspT107I GGYRCC 2 cut(s) 555, 1784
BspTI CTTAAG 1 cut(s) 3458
BsrBI CCGCTC 3 cut(s) 840, 1758, 3052
BsrDI GCAATG 5 cut(s) 821, 1205, 1285, 3805, 4218
BsrFI RCCGGY 3 cut(s) 558, 2275, 4052
BssAI RCCGGY 3 cut(s) 558, 2275, 4052
BssECI CCNNGG 6 cut(s) 174, 1008, 2840, 2973, 3369, 4347
BssNAI GTATAC 1 cut(s) 2477
BssSI CACGAG 1 cut(s) 1067
BssT1I CCWWGG 2 cut(s) 2840, 4347
Bst1107I GTATAC 1 cut(s) 2477
Bst2BI CACGAG 1 cut(s) 1067
Bst2UI CCWGG 6 cut(s) 2216, 2831, 2995, 3371, 4010, 4406
Bst4CI ACNGT 9 cut(s) 64, 126, 454, 1095, 1218, 2074, 2616, 3071, 3472
Bst6I CTCTTC 1 cut(s) 3093
BstAFI CTTAAG 1 cut(s) 3458
BstENI CCTNNNNNAGG 1 cut(s) 3273
BstFNI CGCG 2 cut(s) 1618, 3693
BstH2I RGCGCY 1 cut(s) 3500
BstHHI GCGC 1 cut(s) 3499
BstMAI GTCTC 6 cut(s) 921, 1319, 1916, 2604, 3077, 4021
BstNI CCWGG 6 cut(s) 2216, 2831, 2995, 3371, 4010, 4406
BstNSI RCATGY 6 cut(s) 1809, 1959, 2551, 3256, 3717, 3932
BstSCI CCNGG 8 cut(s) 1267, 2214, 2829, 2972, 2993, 3369, 4008, 4404
BstSFI CTRYAG 5 cut(s) 13, 1299, 1351, 1914, 2871
BstSLI GKGCMC 2 cut(s) 376, 4417
BstUI CGCG 2 cut(s) 1618, 3693
BstX2I RGATCY 5 cut(s) 155, 988, 2438, 2706, 3161
BstXI CCANNNNNNTGG 2 cut(s) 3211, 3632
BstYI RGATCY 5 cut(s) 155, 988, 2438, 2706, 3161
BstZ17I GTATAC 1 cut(s) 2477
Bsu36I CCTNAGG 2 cut(s) 993, 1488
BsuI GTATCC 1 cut(s) 1013
BsuRI GGCC 7 cut(s) 655, 1431, 1515, 2275, 2506, 3302, 3609
BtrI CACGTC 1 cut(s) 2960
BtsI GCAGTG 2 cut(s) 204, 2004
BveI ACCTGC 3 cut(s) 2488, 2803, 4373
CaiI CAGNNNCTG 1 cut(s) 1307
CciI TCATGA 3 cut(s) 589, 2299, 4102
CfoI GCGC 1 cut(s) 3499
Cfr10I RCCGGY 3 cut(s) 558, 2275, 4052
Cfr13I GGNCC 5 cut(s) 28, 866, 2374, 2520, 3919
Csp6I GTAC 9 cut(s) 736, 1091, 1103, 1118, 1785, 1965, 2742, 3140, 3263
CviQI GTAC 9 cut(s) 736, 1091, 1103, 1118, 1785, 1965, 2742, 3140, 3263
DraI TTTAAA 2 cut(s) 2229, 3172
DraIII CACNNNGTG 1 cut(s) 3826
DrdI GACNNNNNNGTC 1 cut(s) 1474
DseDI GACNNNNNNGTC 1 cut(s) 1474
EaeI YGGCCR 1 cut(s) 1513
Eam1104I CTCTTC 1 cut(s) 3093
EarI CTCTTC 1 cut(s) 3093
Ecl136II GAGCTC 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 2840, 4347
Eco147I AGGCCT 1 cut(s) 3302
Eco24I GRGCYC 1 cut(s) 527
Eco32I GATATC 1 cut(s) 1905
Eco47I GGWCC 5 cut(s) 28, 866, 2374, 2520, 3919
Eco47III AGCGCT 1 cut(s) 3498
Eco53kI GAGCTC 1 cut(s) 525
Eco57I CTGAAG 7 cut(s) 168, 1953, 2801, 3009, 3567, 3717, 3797
Eco81I CCTNAGG 2 cut(s) 993, 1488
Eco88I CYCGRG 1 cut(s) 1007
EcoICRI GAGCTC 1 cut(s) 525
EcoNI CCTNNNNNAGG 1 cut(s) 3273
EcoRI GAATTC 2 cut(s) 322, 3314
EcoRII CCWGG 6 cut(s) 2214, 2829, 2993, 3369, 4008, 4404
EcoRV GATATC 1 cut(s) 1905
EcoT14I CCWWGG 2 cut(s) 2840, 4347
EcoT22I ATGCAT 2 cut(s) 2751, 3437
EcoT38I GRGCYC 1 cut(s) 527
ErhI CCWWGG 2 cut(s) 2840, 4347
Esp3I CGTCTC 1 cut(s) 921
FalI AAGNNNNNCTT 2 cut(s) 4288, 4320
FaqI GGGAC 3 cut(s) 332, 1593, 2892
FauI CCCGC 1 cut(s) 4114
FauNDI CATATG 1 cut(s) 2648
FbaI TGATCA 6 cut(s) 90, 190, 328, 616, 1063, 3019
FblI GTMKAC 2 cut(s) 2196, 2476
FriOI GRGCYC 1 cut(s) 527
FspBI CTAG 7 cut(s) 203, 2240, 2486, 3416, 3560, 3959, 4418
GlaI GCGC 1 cut(s) 3498
GsaI CCCAGC 2 cut(s) 3015, 4152
GsuI CTGGAG 4 cut(s) 363, 704, 754, 3912
HaeII RGCGCY 1 cut(s) 3500
HaeIII GGCC 7 cut(s) 655, 1431, 1515, 2275, 2506, 3302, 3609
HapII CCGG 6 cut(s) 559, 656, 1268, 2276, 2973, 4053
HhaI GCGC 1 cut(s) 3499
Hin6I GCGC 1 cut(s) 3497
HinP1I GCGC 1 cut(s) 3497
HincII GTYRAC 1 cut(s) 3520
HindII GTYRAC 1 cut(s) 3520
HindIII AAGCTT 4 cut(s) 269, 3174, 3455, 4064
HpaII CCGG 6 cut(s) 559, 656, 1268, 2276, 2973, 4053
HphI GGTGA 4 cut(s) 710, 1155, 1881, 3720
HpyCH4III ACNGT 9 cut(s) 64, 126, 454, 1095, 1218, 2074, 2616, 3071, 3472
HpyCH4IV ACGT 3 cut(s) 930, 2959, 3522
HpySE526I ACGT 3 cut(s) 930, 2959, 3522
HspAI GCGC 1 cut(s) 3497
KpnI GGTACC 1 cut(s) 1788
Ksp22I TGATCA 6 cut(s) 90, 190, 328, 616, 1063, 3019
LguI GCTCTTC 1 cut(s) 3093
MaeI CTAG 7 cut(s) 203, 2240, 2486, 3416, 3560, 3959, 4418
MaeII ACGT 3 cut(s) 930, 2959, 3522
MbiI CCGCTC 3 cut(s) 840, 1758, 3052
MfeI CAATTG 2 cut(s) 798, 2799
MflI RGATCY 5 cut(s) 155, 988, 2438, 2706, 3161
MhlI GDGCHC 6 cut(s) 376, 527, 3295, 3470, 3699, 4417
MlsI TGGCCA 1 cut(s) 1515
MluNI TGGCCA 1 cut(s) 1515
MlyI GAGTC 8 cut(s) 354, 641, 710, 811, 1120, 1298, 2021, 2192
MmeI TCCRAC 5 cut(s) 961, 1983, 2155, 2226, 4237
Mox20I TGGCCA 1 cut(s) 1515
Mph1103I ATGCAT 2 cut(s) 2751, 3437
MroXI GAANNNNTTC 7 cut(s) 576, 2081, 2616, 2673, 2906, 3801, 4303
MscI TGGCCA 1 cut(s) 1515
MslI CAYNNNNRTG 8 cut(s) 394, 443, 1960, 3209, 3251, 3473, 3630, 3893
Msp20I TGGCCA 1 cut(s) 1515
MspCI CTTAAG 1 cut(s) 3458
MspI CCGG 6 cut(s) 559, 656, 1268, 2276, 2973, 4053
MspR9I CCNGG 8 cut(s) 1269, 2216, 2831, 2974, 2995, 3371, 4010, 4406
MunI CAATTG 2 cut(s) 798, 2799
Mva1269I GAATGC 2 cut(s) 3191, 3862
MvaI CCWGG 6 cut(s) 2216, 2831, 2995, 3371, 4010, 4406
MvnI CGCG 2 cut(s) 1618, 3693
NciI CCSGG 2 cut(s) 1269, 2974
NdeI CATATG 1 cut(s) 2648
NheI GCTAGC 1 cut(s) 202
NmuCI GTSAC 5 cut(s) 702, 1808, 2962, 3482, 3709
NsiI ATGCAT 2 cut(s) 2751, 3437
NspI RCATGY 6 cut(s) 1809, 1959, 2551, 3256, 3717, 3932
OliI CACNNNNGTG 2 cut(s) 3473, 3893
PaeI GCATGC 1 cut(s) 2551
PagI TCATGA 3 cut(s) 589, 2299, 4102
PceI AGGCCT 1 cut(s) 3302
PciI ACATGT 2 cut(s) 1805, 3928
PciSI GCTCTTC 1 cut(s) 3093
PctI GAATGC 2 cut(s) 3191, 3862
PdmI GAANNNNTTC 7 cut(s) 576, 2081, 2616, 2673, 2906, 3801, 4303
PflMI CCANNNNNTGG 1 cut(s) 2019
PfoI TCCNGGA 1 cut(s) 4008
PleI GAGTC 8 cut(s) 353, 640, 709, 811, 1119, 1298, 2021, 2192
PpsI GAGTC 8 cut(s) 353, 640, 709, 811, 1119, 1298, 2021, 2192
PscI ACATGT 2 cut(s) 1805, 3928
PshBI ATTAAT 1 cut(s) 2445
Psp124BI GAGCTC 1 cut(s) 527
Psp6I CCWGG 6 cut(s) 2214, 2829, 2993, 3369, 4008, 4404
PspFI CCCAGC 2 cut(s) 3011, 4148
PspGI CCWGG 6 cut(s) 2214, 2829, 2993, 3369, 4008, 4404
PspPI GGNCC 5 cut(s) 28, 866, 2374, 2520, 3919
PstI CTGCAG 2 cut(s) 1303, 1918
PstNI CAGNNNCTG 1 cut(s) 1307
PsuI RGATCY 5 cut(s) 155, 988, 2438, 2706, 3161
RsaI GTAC 9 cut(s) 737, 1092, 1104, 1119, 1786, 1966, 2743, 3141, 3264
RsaNI GTAC 9 cut(s) 736, 1091, 1103, 1118, 1785, 1965, 2742, 3140, 3263
RseI CAYNNNNRTG 8 cut(s) 394, 443, 1960, 3209, 3251, 3473, 3630, 3893
SacI GAGCTC 1 cut(s) 527
SapI GCTCTTC 1 cut(s) 3093
Sau96I GGNCC 5 cut(s) 28, 866, 2374, 2520, 3919
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 8 cut(s) 354, 641, 710, 811, 1120, 1298, 2021, 2192
ScrFI CCNGG 8 cut(s) 1269, 2216, 2831, 2974, 2995, 3371, 4010, 4406
SduI GDGCHC 6 cut(s) 376, 527, 3295, 3470, 3699, 4417
SfcI CTRYAG 5 cut(s) 13, 1299, 1351, 1914, 2871
SinI GGWCC 5 cut(s) 28, 866, 2374, 2520, 3919
SmiMI CAYNNNNRTG 8 cut(s) 394, 443, 1960, 3209, 3251, 3473, 3630, 3893
SmlI CTYRAG 9 cut(s) 905, 1713, 1908, 2402, 3002, 3191, 3458, 3882, 4094
SmoI CTYRAG 9 cut(s) 905, 1713, 1908, 2402, 3002, 3191, 3458, 3882, 4094
SphI GCATGC 1 cut(s) 2551
SseBI AGGCCT 1 cut(s) 3302
SspI AATATT 1 cut(s) 2157
SspMI CTAG 7 cut(s) 203, 2240, 2486, 3416, 3560, 3959, 4418
SstI GAGCTC 1 cut(s) 527
StuI AGGCCT 1 cut(s) 3302
StyD4I CCNGG 8 cut(s) 1267, 2214, 2829, 2972, 2993, 3369, 4008, 4404
StyI CCWWGG 2 cut(s) 2840, 4347
TaaI ACNGT 9 cut(s) 64, 126, 454, 1095, 1218, 2074, 2616, 3071, 3472
TaiI ACGT 3 cut(s) 933, 2962, 3525
TaqI TCGA 7 cut(s) 246, 842, 1114, 1394, 2088, 2610, 3338
TatI WGTACW 8 cut(s) 735, 1090, 1102, 1117, 1964, 2741, 3139, 3262
TauI GCSGC 5 cut(s) 840, 1621, 3055, 4326, 4355
TseFI GTSAC 5 cut(s) 702, 1808, 2962, 3482, 3709
Tsp45I GTSAC 5 cut(s) 702, 1808, 2962, 3482, 3709
TspGWI ACGGA 3 cut(s) 487, 1753, 4380
Van91I CCANNNNNTGG 1 cut(s) 2019
Vha464I CTTAAG 1 cut(s) 3458
VpaK11BI GGWCC 5 cut(s) 28, 866, 2374, 2520, 3919
VspI ATTAAT 1 cut(s) 2445
XagI CCTNNNNNAGG 1 cut(s) 3273
XapI RAATTY 4 cut(s) 322, 967, 3314, 3801
XceI RCATGY 6 cut(s) 1809, 1959, 2551, 3256, 3717, 3932
XmiI GTMKAC 2 cut(s) 2196, 2476
XmnI GAANNNNTTC 7 cut(s) 576, 2081, 2616, 2673, 2906, 3801, 4303
XspI CTAG 7 cut(s) 203, 2240, 2486, 3416, 3560, 3959, 4418
ZrmI AGTACT 1 cut(s) 737
Zsp2I ATGCAT 2 cut(s) 2751, 3437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.