MD15G1191200.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001838
Physical Location & Seq
Reverse (-)
15027688 .. 15034723
7036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1191200.v1.1.491

Sequence Viewer

Length: 4029 bp
ATGACCGCCACCATGACCTCCGCCTCATCCTCCCAAATCCTCGGCCACACTGCCACCTTCATCTCCAAAACCCTATCCCAACCCGAGCTCCGCCGCCACCTCTTCTCCACTCTCCGCCGTAAACTCTCTCCCTCCGCCTCCCAAGCCGCCCTCAAACCCTTAAACCTGGCCGCGGAAACCCTGGAAAACGCAATCTCCACCACCAGCCCCGCCATTTGTGCCTCCTCCCTCACCCTCTCCGAAAAGCTCCTCCGGTCAACCCCCAAAAACCTCTTCTCCTCCCTCCTCCTCTCCCTCATCTACGGTCTCTCCAGACGCCCCATTGACGCTGCGCTCAGCCTCCTAGACATCTTCCACACCGACCCTTCATTCGCCCGAACGGAACTCGCCCCCGTACTATTTGAAGAGCTCTTTCTAGTCCACCTCCTCCCTGTCGTCCATTGGTACAACGAACAAAGATCACAAATATTACCAACTTTGTCCCCGAACATTAGCTATGAAAATGACGACTGTTCTATATCCGACATGTCCGTCGTGTTTTCGTGCTCGAAATCGTTGTCTAAGATGAGTGGGGGGCAGACTTCGGAGCTAAAGGAGTTGGAATCTAATTACGAGAAAGTTCTTGATGAAAATTGCAGGGTTTTTAGTAATTATTTTAAACAAGTTCTGGAAAATAAAGATGCGAGTAGACGGATTGATCTGCCCGCAGTGATTTTGAAGAGAGTTGAAGGACTTAATGAAGTCAAGAATGGAGATGATGATGATGAACAAGGGATCAAAATGGGGGAACCCGGATTCGAAAACGGACGGTATAATCCAATATGGGCTGAAGCAGAAAGACCAGTTGAAGTACTCAGCAGCAGAAGAAAACCAAAGTCTCCACCTTTTTATCCTCAAAGAGTTTCAATCAGCAATATCCTCAAAACCCAGGAACAGGAACCTTCCTGGAACTCGAAATCTTCGCCCAATGTGAATTCTTCAGATTCTGAGTCAGAGTCTTCCCTGGAGGATATTTCGGTAAACTCTTCTTCTTCCTTGGGTTACGGAGCAGAAATTGAGGAGGAAAACAACAGAGAAATGGAATTGTTTGAGGCTACAGAGAGCCAAATAAAGAAGCTAAAGCAACCCATTTCTGCAGAATCCAGCCGTTCTCCAGACCAGCTGATCATGGCAGATTATGATAACAATTCTGTTGGGGGAAAAGTACATACACCTCCCAAGGATTTTGTGTGTCCCATTACTAGCACTTTGTTTGATGATCCTGTGACACTCGAGACGGGGCAGACATATGAGCGAAAAGCGATCCAGGAGTGGATCGAAAGAGGGAACTCTACGTGCCCAATTACGCGCCAGAACTTGCAGAGCACTCAACTGCCTAAGACCAACTATGTGCTCAAAAGGCTTATTGCAAGCTGGCAAGAACAGAATCCTAGCGCAGTGTTGAGCCAATCGGAGAGTACAAGCCCATTGATTGAGCCTGTTGTGAAGGCCATAATGCCTTCAACTTCTCCTGACAGTGTTATAACCCAAGGCGGGTTTGATGGCGCAGTTGGTGAGTTAAGACTTGCAATTACCAACCTTTGTATGTCAGAAATTCTCAAGGAGTCTGAAATGGCTGTTCTTCGAATTGAGCGGTTCTGGCAGGAGGCAAATGTGGAATGGGATATTCAGAATTTGCTCACGAAACCTCCAGTGATCAATGGTTTTGTGGAGGTTCTTTTCAATTCTGTTGATCCCAGGGTTCTGTTAGCTGCAGTTTTTCTCCTGTCTGAGCTAGGGTCAAGAGACAATGCTGTGATCCAGACGCTAACGCGCGTTGATTCTGATGTTGAATGCATTGTGGCGCTTTTTAAAAAGGGGTTGATGGAGGCTGTGGTGTTGATATATCTGTTGAGGCATTCGATCCTGAACCTAATTGAGATGGGCATTGTGGAGTCTCTCTTACTGGTTATCAAAAGGAAAGACGATGATTTGCTTAACATGTGTCTAAAGCCGAGAACAGCTGCAGTGGTTTTACTCGGGCTGATTCTTGGTGGCAGTGAAGAGGGTATTGCCTCTTCGATTGTTAACACAGTGGTTTCTGAAAAAACAACAATTATTAGAAGTTTGGAATCTGAATCGACCGAAGAGAGGGTTGCAGCAGTTAGGATCTTGTTGAGATGTATGCAGCAGGATGGAAAGTGCAGGAACACAATAGCTGATAAAGCCGAGCTGGCTCCTGTTTTGGATAGCTTTGTCGGCGCAAGTGATGGCGAAAAGTTTGAGATAGTTCACTTTTTCTCCGAGTTAGTTAAATTAAACAGAAGGACATTTAATGAACAAATCCTGCATATCATAAAGGATGAAGGCTCTGTCAGCTCAATGCACGCCCTTCTTATTTATCTGCAGACTGCCCCTCAAGATCAGTGTCCTATTGTGGCCGGTCTACTACTTCAACTCGATCTTCTGGCAGAGCCGAGAAAGATGAGCATATACCGCGAAGAGGCAATAGACACGCTCATCTCATGTCTCAGAAATGCGGACTTCCCTACTGCCCAAATTGCTGCTGCTGAGACAATTATGTCGCTGCAAGGGAGGTTCACCACATCCGGAAGGCCTCTCACCAGGGCTATCCTTCTCAAGCGTACTGGCCTTGACAAAAGTTACAAAAGTCACGTACGAATGGATCAACTTAGCAATTTTTCTGGAGATGACGAAACTCTTGAGGAAGAGAAGGCGGCTGATAATTGGGAAAGAAAAATGGCATTTGTTTTAGCAAGCCATGAGTTTGGTCTACTTTTTGAGGCTTTAGCAGAAGGCTTGAAGAGCAGATATGCGGAATTATGTTCAGCGTGCTTTGTGATAGCCACATGGCTCGTGCACATGCTCAATGTTCTTCCAGACACAGGAATACGTGGAGCAGCCCGCGTCTGCTTGCTCAAACGTTACATGTCGATATTCAAGTCTGCCAAGGACACCGATGACAAAGCCCTCTCTATGCTTGCGTTGAGCAGCTTCATCCAAGATCCTGAGGGGATGCAAGAGGTAACTTCTTCCATAAAGGATATCGTAAAAGGCCTGAGAGAGCTTAAGAGATCGACTCCCTTGGCATTTCAAATGCTAAAACTATTTTCTGAGGGGCAAGACTCTAGTGCAGAACTGTGGGATCATAAAGAATTAGTTCAAGTAGATTGCAGCGAGAATGGAGAGGTTCTTTCAGTCATTTGCTTCAAGGACAAAATATTTTCGGGCCACTCAGATGGAACTATAAAGGTATGGACGGGTAAAGGTAGCGTTCTTCATCTAATCCAAGAAACTCGAGAACACACAAAGGCAGTTACTAGCTTGGCAATTTTGCAATCGGGGGAAGTTTTATACAGTGGCTCGCTTGATAGAACTACAAGGGTATGGTCTATTAGCAATGAAGCAATGTACTGTGTACAAGTACACGATATGAAGGATCAAGTTCATAGTTTAGCCGTCACGAATAGCCTCGCTTGCTTCATTCCACAAAGTACCGGTATCAAGGTTCATTCGTGGAATGGAGGAACCAAATTGTTAAATTCAAGTAAACATGTGAGGTGCTTCGCTCTAGTACATGGAAAATTATATTGTGGATGCAATGACAATGGTATTCAGGAGATTGATTTGGCCACGGGAACACTCAGTACCATTCAAAATGGCACTCGGAAACTACTCACGAAATCAAACCCTATCCACGCCATTCAAGTTCACAGTGGACTTATCTATACAGCCAGTTCTTCCGCGGATGGGGCAGCTGTTAAGATATGGAATGCTGCAAACTTCAACCTGGTTGGATCACTTCCGACCACATTGGAAGTGCGGGCGATGGCCATAAGCTCGGAACTGATTTATTTGGGAGGTAAAGGAGGAAGTGTGGAAATATGGGATAGGAATAAGCAGAACAGAATAGACACCCTACAAACTGGTGCCAATTGCAAGGTCCTTTGCTTGGCTCTTGATGCCAATGAGGACGTTTTGGTCACTGGAACTTCTGATGGCCGAATTCGGGCATGGGGACTCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1343

Amino Acids

148.2

Weight (kDa)

5.45

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 15 - 231 3.1e-22 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 406 - 476 8.3e-17 U-box domain
ARM_LIN_2nd PF23654 516 - 673 2.4e-59 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 676 - 1038 2.3e-144 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR5 PF25175 1036 - 1175 8e-09 WDR5 beta-propeller domain
WD40_Prp19 PF24814 1037 - 1149 1.6e-08 Prp19 WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 1040 - 1143 9e-06 WDR90/POC16, second beta-propeller
WD40_CDC20-Fz PF24807 1042 - 1153 2.5e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1047 - 1174 3.9e-10 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 1063 - 1156 9.2e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 1071 - 1158 8e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 1071 - 1258 1.3e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 1072 - 1136 1.5e-07 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 1072 - 1280 1.3e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 1182 - 1340 7.8e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 1228 - 1340 9e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1243 - 1341 8.9e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1274 - 1340 2.5e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1523
AasI GACNNNNNNGTC 3 cut(s) 530, 2560, 3980
AccB1I GGYRCC 1 cut(s) 3929
AccBSI CCGCTC 1 cut(s) 1633
AccI GTMKAC 3 cut(s) 688, 2425, 2773
AccII CGCG 7 cut(s) 173, 1348, 1813, 1815, 2478, 2907, 3746
AccIII TCCGGA 1 cut(s) 2588
AclI AACGTT 1 cut(s) 2923
AcoI YGGCCR 6 cut(s) 43, 168, 2418, 3630, 3831, 4000
AcsI RAATTY 5 cut(s) 973, 1593, 1672, 3541, 4005
AcuI CTGAAG 2 cut(s) 849, 963
AcyI GRCGYC 1 cut(s) 316
AfiI CCNNNNNNNGG 9 cut(s) 172, 934, 1533, 2130, 2482, 2885, 3750, 3952, 4009
AflII CTTAAG 1 cut(s) 3068
AflIII ACRYGT 4 cut(s) 525, 1980, 2928, 3553
AgeI ACCGGT 1 cut(s) 3497
AjnI CCWGG 9 cut(s) 165, 180, 927, 944, 1002, 1305, 1736, 2603, 3789
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
Alw21I GWGCWC 6 cut(s) 90, 411, 548, 1367, 1395, 2862
Alw26I GTCTC 7 cut(s) 311, 882, 1268, 1779, 1941, 2513, 2546
Alw44I GTGCAC 1 cut(s) 2858
AlwNI CAGNNNCTG 1 cut(s) 986
Ama87I CYCGRG 4 cut(s) 83, 1271, 2018, 3297
Aor13HI TCCGGA 1 cut(s) 2588
ApaLI GTGCAC 1 cut(s) 2858
ApoI RAATTY 5 cut(s) 973, 1593, 1672, 3541, 4005
ArsI GACNNNNNNTTYG 4 cut(s) 353, 385, 542, 574
AsiGI ACCGGT 1 cut(s) 3497
Asp700I GAANNNNTTC 1 cut(s) 3159
AspLEI GCGC 7 cut(s) 334, 1350, 1436, 1547, 1815, 1846, 2243
AspS9I GGNCC 2 cut(s) 3229, 3943
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 4 cut(s) 223, 1565, 2572, 2593
AsuII TTCGAA 2 cut(s) 798, 1624
AvaI CYCGRG 4 cut(s) 83, 1271, 2018, 3297
AvaII GGWCC 1 cut(s) 3943
AxyI CCTNAGG 1 cut(s) 3009
BaeGI GKGCMC 2 cut(s) 1340, 2862
BalI TGGCCA 2 cut(s) 3632, 3833
BanI GGYRCC 1 cut(s) 3929
BanII GRGCYC 2 cut(s) 90, 411
BarI GAAGNNNNNNTAC 2 cut(s) 2041, 2073
BauI CACGAG 1 cut(s) 2855
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 6 cut(s) 90, 411, 548, 1367, 1395, 2862
BccI CCATC 9 cut(s) 1535, 1858, 1915, 2168, 2243, 3233, 3743, 3823, 3992
BceAI ACGGC 3 cut(s) 102, 1131, 3443
BciT130I CCWGG 9 cut(s) 167, 182, 929, 946, 1004, 1307, 1738, 2605, 3791
BclI TGATCA 2 cut(s) 1164, 1695
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 7 cut(s) 311, 882, 1268, 1779, 1941, 2513, 2546
BfaI CTAG 8 cut(s) 344, 416, 1242, 1431, 1775, 3129, 3321, 3572
BfmI CTRYAG 5 cut(s) 1095, 1134, 1752, 2004, 2384
BfoI RGCGCY 1 cut(s) 1847
BfrI CTTAAG 1 cut(s) 3068
BglI GCCNNNNNGGC 1 cut(s) 2213
BlpI GCTNAGC 1 cut(s) 335
BmcAI AGTACT 1 cut(s) 852
Bme18I GGWCC 1 cut(s) 3943
BmeT110I CYCGRG 4 cut(s) 83, 1271, 2018, 3297
BmgT120I GGNCC 2 cut(s) 3229, 3943
BmiI GGNNCC 5 cut(s) 789, 939, 2217, 3529, 3931
BmsI GCATC 4 cut(s) 670, 3006, 3587, 3952
BpiI GAAGAC 1 cut(s) 990
BplI GAGNNNNNCTC 2 cut(s) 3064, 3096
BpmI CTGGAG 5 cut(s) 295, 1025, 1137, 1674, 2706
Bpu1102I GCTNAGC 1 cut(s) 335
Bpu14I TTCGAA 2 cut(s) 798, 1624
BpuEI CTTGAG 4 cut(s) 1583, 2382, 2603, 2723
BpuMI CCSGG 1 cut(s) 792
BsaAI YACGTR 3 cut(s) 1335, 2656, 2894
BsaHI GRCGYC 1 cut(s) 316
BsaI GGTCTC 1 cut(s) 311
BsaWI WCCGGW 3 cut(s) 252, 2588, 3497
Bsc4I CCNNNNNNNGG 9 cut(s) 172, 934, 1533, 2130, 2482, 2885, 3750, 3952, 4009
Bse118I RCCGGY 2 cut(s) 2420, 3497
Bse1I ACTGG 7 cut(s) 842, 1691, 1950, 2632, 3735, 3931, 3991
Bse21I CCTNAGG 1 cut(s) 3009
Bse3DI GCAATG 3 cut(s) 3406, 3414, 3607
BseAI TCCGGA 1 cut(s) 2588
BseBI CCWGG 9 cut(s) 167, 182, 929, 946, 1004, 1307, 1738, 2605, 3791
BseGI GGATG 8 cut(s) 26, 2179, 2347, 2585, 2997, 3021, 3602, 3754
BseLI CCNNNNNNNGG 9 cut(s) 172, 934, 1533, 2130, 2482, 2885, 3750, 3952, 4009
BseMI GCAATG 3 cut(s) 3406, 3414, 3607
BseNI ACTGG 7 cut(s) 842, 1691, 1950, 2632, 3735, 3931, 3991
BseRI GAGGAG 7 cut(s) 214, 239, 268, 275, 278, 416, 1073
BseSI GKGCMC 2 cut(s) 1340, 2862
BsgI GTGCAG 2 cut(s) 2203, 3153
Bsh1236I CGCG 7 cut(s) 173, 1348, 1813, 1815, 2478, 2907, 3746
Bsh1285I CGRYCG 1 cut(s) 2124
BshNI GGYRCC 1 cut(s) 3929
BshTI ACCGGT 1 cut(s) 3497
BsiEI CGRYCG 1 cut(s) 2124
BsiHKAI GWGCWC 6 cut(s) 90, 411, 548, 1367, 1395, 2862
BsiHKCI CYCGRG 4 cut(s) 83, 1271, 2018, 3297
BsiSI CCGG 5 cut(s) 253, 792, 2421, 2589, 3498
BsiWI CGTACG 1 cut(s) 2656
BslFI GGGAC 2 cut(s) 466, 1218
BslI CCNNNNNNNGG 9 cut(s) 172, 934, 1533, 2130, 2482, 2885, 3750, 3952, 4009
BsmAI GTCTC 7 cut(s) 311, 882, 1268, 1779, 1941, 2513, 2546
BsmBI CGTCTC 1 cut(s) 1268
BsmFI GGGAC 2 cut(s) 466, 1218
BsmI GAATGC 3 cut(s) 1838, 1897, 3778
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 4 cut(s) 83, 1271, 2018, 3297
Bsp119I TTCGAA 2 cut(s) 798, 1624
Bsp1286I GDGCHC 7 cut(s) 90, 411, 548, 1340, 1367, 1395, 2862
Bsp13I TCCGGA 1 cut(s) 2588
Bsp1407I TGTACA 1 cut(s) 3418
Bsp1720I GCTNAGC 1 cut(s) 335
BspEI TCCGGA 1 cut(s) 2588
BspFNI CGCG 7 cut(s) 173, 1348, 1813, 1815, 2478, 2907, 3746
BspLI GGNNCC 5 cut(s) 789, 939, 2217, 3529, 3931
BspMAI CTGCAG 4 cut(s) 1138, 1756, 2008, 2388
BspQI GCTCTTC 2 cut(s) 399, 2798
BspT104I TTCGAA 2 cut(s) 798, 1624
BspT107I GGYRCC 1 cut(s) 3929
BspTI CTTAAG 1 cut(s) 3068
BspTNI GGTCTC 1 cut(s) 311
BsrBI CCGCTC 1 cut(s) 1633
BsrDI GCAATG 3 cut(s) 3406, 3414, 3607
BsrFI RCCGGY 2 cut(s) 2420, 3497
BsrGI TGTACA 1 cut(s) 3418
BsrI ACTGG 7 cut(s) 842, 1691, 1950, 2632, 3735, 3931, 3991
BssAI RCCGGY 2 cut(s) 2420, 3497
BssNI GRCGYC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 2855
BssT1I CCWWGG 5 cut(s) 1035, 1218, 1528, 2949, 3084
Bst2BI CACGAG 1 cut(s) 2855
Bst2UI CCWGG 9 cut(s) 167, 182, 929, 946, 1004, 1307, 1738, 2605, 3791
Bst4CI ACNGT 9 cut(s) 305, 512, 810, 1517, 2074, 3141, 3359, 3416, 3716
BstACI GRCGYC 1 cut(s) 316
BstAFI CTTAAG 1 cut(s) 3068
BstAUI TGTACA 1 cut(s) 3418
BstBAI YACGTR 3 cut(s) 1335, 2656, 2894
BstBI TTCGAA 2 cut(s) 798, 1624
BstDSI CCRYGG 3 cut(s) 171, 3633, 3744
BstF5I GGATG 8 cut(s) 26, 2179, 2347, 2585, 2997, 3021, 3602, 3754
BstFNI CGCG 7 cut(s) 173, 1348, 1813, 1815, 2478, 2907, 3746
BstH2I RGCGCY 1 cut(s) 1847
BstHHI GCGC 7 cut(s) 334, 1350, 1436, 1547, 1815, 1846, 2243
BstMAI GTCTC 7 cut(s) 311, 882, 1268, 1779, 1941, 2513, 2546
BstMCI CGRYCG 1 cut(s) 2124
BstNI CCWGG 9 cut(s) 167, 182, 929, 946, 1004, 1307, 1738, 2605, 3791
BstNSI RCATGY 5 cut(s) 529, 1984, 2866, 2932, 3557
BstSFI CTRYAG 5 cut(s) 1095, 1134, 1752, 2004, 2384
BstSLI GKGCMC 2 cut(s) 1340, 2862
BstUI CGCG 7 cut(s) 173, 1348, 1813, 1815, 2478, 2907, 3746
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 2 cut(s) 2148, 3004
BstXI CCANNNNNNTGG 2 cut(s) 2768, 3239
BstYI RGATCY 2 cut(s) 2148, 3004
Bsu36I CCTNAGG 1 cut(s) 3009
BtgI CCRYGG 3 cut(s) 171, 3633, 3744
BtgZI GCGATG 1 cut(s) 3842
BtsCI GGATG 8 cut(s) 26, 2179, 2347, 2585, 2997, 3021, 3602, 3754
BtsI GCAGTG 5 cut(s) 48, 714, 1443, 2013, 2044
CaiI CAGNNNCTG 1 cut(s) 986
CfoI GCGC 7 cut(s) 334, 1350, 1436, 1547, 1815, 1846, 2243
Cfr10I RCCGGY 2 cut(s) 2420, 3497
Cfr13I GGNCC 2 cut(s) 3229, 3943
Cfr42I CCGCGG 2 cut(s) 174, 3747
CseI GACGC 4 cut(s) 324, 335, 1813, 2896
CsiI ACCWGGT 1 cut(s) 3789
CspAI ACCGGT 1 cut(s) 3497
DraI TTTAAA 2 cut(s) 658, 1852
DrdI GACNNNNNNGTC 3 cut(s) 530, 2560, 3980
DseDI GACNNNNNNGTC 3 cut(s) 530, 2560, 3980
EaeI YGGCCR 6 cut(s) 43, 168, 2418, 3630, 3831, 4000
EciI GGCGGA 4 cut(s) 10, 80, 104, 124
Ecl136II GAGCTC 2 cut(s) 88, 409
Eco130I CCWWGG 5 cut(s) 1035, 1218, 1528, 2949, 3084
Eco147I AGGCCT 2 cut(s) 2596, 3057
Eco24I GRGCYC 2 cut(s) 90, 411
Eco31I GGTCTC 1 cut(s) 311
Eco32I GATATC 1 cut(s) 3046
Eco47I GGWCC 1 cut(s) 3943
Eco53kI GAGCTC 2 cut(s) 88, 409
Eco57I CTGAAG 2 cut(s) 849, 963
Eco81I CCTNAGG 1 cut(s) 3009
Eco88I CYCGRG 4 cut(s) 83, 1271, 2018, 3297
EcoICRI GAGCTC 2 cut(s) 88, 409
EcoO109I RGGNCCY 1 cut(s) 3943
EcoRI GAATTC 2 cut(s) 973, 4005
EcoRII CCWGG 9 cut(s) 165, 180, 927, 944, 1002, 1305, 1736, 2603, 3789
EcoRV GATATC 1 cut(s) 3046
EcoT14I CCWWGG 5 cut(s) 1035, 1218, 1528, 2949, 3084
EcoT22I ATGCAT 1 cut(s) 1838
EcoT38I GRGCYC 2 cut(s) 90, 411
ErhI CCWWGG 5 cut(s) 1035, 1218, 1528, 2949, 3084
Esp3I CGTCTC 1 cut(s) 1268
FaqI GGGAC 2 cut(s) 466, 1218
FauI CCCGC 5 cut(s) 217, 712, 1526, 2912, 3816
FauNDI CATATG 1 cut(s) 1288
FbaI TGATCA 2 cut(s) 1164, 1695
FblI GTMKAC 3 cut(s) 688, 2425, 2773
FokI GGATG 8 cut(s) 13, 2186, 2354, 2572, 2984, 3028, 3609, 3761
FriOI GRGCYC 2 cut(s) 90, 411
FspBI CTAG 8 cut(s) 344, 416, 1242, 1431, 1775, 3129, 3321, 3572
GlaI GCGC 7 cut(s) 333, 1349, 1435, 1546, 1814, 1845, 2242
GsuI CTGGAG 5 cut(s) 295, 1025, 1137, 1674, 2706
HaeII RGCGCY 1 cut(s) 1847
HapII CCGG 5 cut(s) 253, 792, 2421, 2589, 3498
HgaI GACGC 4 cut(s) 324, 335, 1813, 2896
HhaI GCGC 7 cut(s) 334, 1350, 1436, 1547, 1815, 1846, 2243
Hin1I GRCGYC 1 cut(s) 316
Hin6I GCGC 7 cut(s) 332, 1348, 1434, 1545, 1813, 1844, 2241
HinP1I GCGC 7 cut(s) 332, 1348, 1434, 1545, 1813, 1844, 2241
HincII GTYRAC 2 cut(s) 258, 2068
HindII GTYRAC 2 cut(s) 258, 2068
HpaI GTTAAC 1 cut(s) 2068
HpaII CCGG 5 cut(s) 253, 792, 2421, 2589, 3498
HphI GGTGA 4 cut(s) 223, 1565, 2572, 2593
Hpy99I CGWCG 1 cut(s) 536
HpyCH4III ACNGT 9 cut(s) 305, 512, 810, 1517, 2074, 3141, 3359, 3416, 3716
HpyCH4IV ACGT 5 cut(s) 1334, 2655, 2893, 2923, 3975
HpySE526I ACGT 5 cut(s) 1334, 2655, 2893, 2923, 3975
Hsp92I GRCGYC 1 cut(s) 316
HspAI GCGC 7 cut(s) 332, 1348, 1434, 1545, 1813, 1844, 2241
Kpn2I TCCGGA 1 cut(s) 2588
Ksp22I TGATCA 2 cut(s) 1164, 1695
KspAI GTTAAC 1 cut(s) 2068
KspI CCGCGG 2 cut(s) 174, 3747
LguI GCTCTTC 2 cut(s) 399, 2798
LmnI GCTCC 6 cut(s) 93, 252, 586, 1046, 2221, 2897
LweI GCATC 4 cut(s) 670, 3006, 3587, 3952
MabI ACCWGGT 1 cut(s) 3789
MaeI CTAG 8 cut(s) 344, 416, 1242, 1431, 1775, 3129, 3321, 3572
MaeII ACGT 5 cut(s) 1334, 2655, 2893, 2923, 3975
MaeIII GTNAC 9 cut(s) 1040, 1264, 2642, 2651, 2924, 3025, 3316, 3460, 3982
MbiI CCGCTC 1 cut(s) 1633
MfeI CAATTG 1 cut(s) 3934
MflI RGATCY 2 cut(s) 2148, 3004
MhlI GDGCHC 7 cut(s) 90, 411, 548, 1340, 1367, 1395, 2862
MlsI TGGCCA 2 cut(s) 3632, 3833
MluNI TGGCCA 2 cut(s) 3632, 3833
MlyI GAGTC 7 cut(s) 998, 1004, 1613, 1943, 3073, 3119, 4014
MmeI TCCRAC 4 cut(s) 546, 579, 3775, 3830
Mox20I TGGCCA 2 cut(s) 3632, 3833
Mph1103I ATGCAT 1 cut(s) 1838
MroI TCCGGA 1 cut(s) 2588
MroXI GAANNNNTTC 1 cut(s) 3159
MscI TGGCCA 2 cut(s) 3632, 3833
MslI CAYNNNNRTG 1 cut(s) 3237
Msp20I TGGCCA 2 cut(s) 3632, 3833
MspA1I CMGCKG 5 cut(s) 173, 1162, 2003, 3746, 3758
MspCI CTTAAG 1 cut(s) 3068
MspI CCGG 5 cut(s) 253, 792, 2421, 2589, 3498
MunI CAATTG 1 cut(s) 3934
Mva1269I GAATGC 3 cut(s) 1838, 1897, 3778
MvaI CCWGG 9 cut(s) 167, 182, 929, 946, 1004, 1307, 1738, 2605, 3791
MvnI CGCG 7 cut(s) 173, 1348, 1813, 1815, 2478, 2907, 3746
NciI CCSGG 1 cut(s) 792
NdeI CATATG 1 cut(s) 1288
NlaIV GGNNCC 5 cut(s) 789, 939, 2217, 3529, 3931
NmeAIII GCCGAG 4 cut(s) 21, 2019, 2233, 2481
NmuCI GTSAC 4 cut(s) 1264, 2651, 3460, 3982
NsiI ATGCAT 1 cut(s) 1838
NspI RCATGY 5 cut(s) 529, 1984, 2866, 2932, 3557
NspV TTCGAA 2 cut(s) 798, 1624
PaeR7I CTCGAG 2 cut(s) 1271, 3297
PasI CCCWGGG 1 cut(s) 1737
PceI AGGCCT 2 cut(s) 2596, 3057
PciI ACATGT 4 cut(s) 525, 1980, 2928, 3553
PciSI GCTCTTC 2 cut(s) 399, 2798
PctI GAATGC 3 cut(s) 1838, 1897, 3778
PdmI GAANNNNTTC 1 cut(s) 3159
PfeI GAWTC 9 cut(s) 602, 795, 983, 1139, 1426, 1820, 2026, 2111, 2117
Pfl23II CGTACG 1 cut(s) 2656
PfoI TCCNGGA 2 cut(s) 944, 1305
PinAI ACCGGT 1 cut(s) 3497
PleI GAGTC 7 cut(s) 997, 1003, 1612, 1942, 3073, 3119, 4014
PpsI GAGTC 7 cut(s) 997, 1003, 1612, 1942, 3073, 3119, 4014
Ppu21I YACGTR 3 cut(s) 1335, 2656, 2894
PpuMI RGGWCCY 1 cut(s) 3943
PscI ACATGT 4 cut(s) 525, 1980, 2928, 3553
PsiI TTATAA 1 cut(s) 1523
Psp124BI GAGCTC 2 cut(s) 90, 411
Psp1406I AACGTT 1 cut(s) 2923
Psp5II RGGWCCY 1 cut(s) 3943
Psp6I CCWGG 9 cut(s) 165, 180, 927, 944, 1002, 1305, 1736, 2603, 3789
PspGI CCWGG 9 cut(s) 165, 180, 927, 944, 1002, 1305, 1736, 2603, 3789
PspLI CGTACG 1 cut(s) 2656
PspN4I GGNNCC 5 cut(s) 789, 939, 2217, 3529, 3931
PspPI GGNCC 2 cut(s) 3229, 3943
PspPPI RGGWCCY 1 cut(s) 3943
PsrI GAACNNNNNNTAC 2 cut(s) 3631, 3663
PstI CTGCAG 4 cut(s) 1138, 1756, 2008, 2388
PstNI CAGNNNCTG 1 cut(s) 986
PsuI RGATCY 2 cut(s) 2148, 3004
PvuII CAGCTG 3 cut(s) 1162, 2003, 3758
RseI CAYNNNNRTG 1 cut(s) 3237
SacI GAGCTC 2 cut(s) 90, 411
SacII CCGCGG 2 cut(s) 174, 3747
SapI GCTCTTC 2 cut(s) 399, 2798
Sau96I GGNCC 2 cut(s) 3229, 3943
ScaI AGTACT 1 cut(s) 852
SchI GAGTC 7 cut(s) 998, 1004, 1613, 1943, 3073, 3119, 4014
SduI GDGCHC 7 cut(s) 90, 411, 548, 1340, 1367, 1395, 2862
SexAI ACCWGGT 1 cut(s) 3789
SfaNI GCATC 4 cut(s) 670, 3006, 3587, 3952
SfcI CTRYAG 5 cut(s) 1095, 1134, 1752, 2004, 2384
Sfr274I CTCGAG 2 cut(s) 1271, 3297
Sfr303I CCGCGG 2 cut(s) 174, 3747
SfuI TTCGAA 2 cut(s) 798, 1624
SgrBI CCGCGG 2 cut(s) 174, 3747
SinI GGWCC 1 cut(s) 3943
SlaI CTCGAG 2 cut(s) 1271, 3297
SmiMI CAYNNNNRTG 1 cut(s) 3237
SmlI CTYRAG 7 cut(s) 1271, 1598, 2397, 2618, 2702, 3068, 3297
SmoI CTYRAG 7 cut(s) 1271, 1598, 2397, 2618, 2702, 3068, 3297
SseBI AGGCCT 2 cut(s) 2596, 3057
SspI AATATT 2 cut(s) 468, 3222
SspMI CTAG 8 cut(s) 344, 416, 1242, 1431, 1775, 3129, 3321, 3572
SstI GAGCTC 2 cut(s) 90, 411
StuI AGGCCT 2 cut(s) 2596, 3057
StyI CCWWGG 5 cut(s) 1035, 1218, 1528, 2949, 3084
TaaI ACNGT 9 cut(s) 305, 512, 810, 1517, 2074, 3141, 3359, 3416, 3716
TaiI ACGT 5 cut(s) 1337, 2658, 2896, 2926, 3978
TaqII GACCGA 1 cut(s) 2138
TatI WGTACW 7 cut(s) 850, 1204, 1457, 3411, 3418, 3424, 3574
TauI GCSGC 4 cut(s) 96, 149, 173, 2720
TfiI GAWTC 9 cut(s) 602, 795, 983, 1139, 1426, 1820, 2026, 2111, 2117
TseFI GTSAC 4 cut(s) 1264, 2651, 3460, 3982
Tsp45I GTSAC 4 cut(s) 1264, 2651, 3460, 3982
TspGWI ACGGA 5 cut(s) 395, 520, 706, 819, 1059
Vha464I CTTAAG 1 cut(s) 3068
VneI GTGCAC 1 cut(s) 2858
VpaK11BI GGWCC 1 cut(s) 3943
XapI RAATTY 5 cut(s) 973, 1593, 1672, 3541, 4005
XceI RCATGY 5 cut(s) 529, 1984, 2866, 2932, 3557
XhoI CTCGAG 2 cut(s) 1271, 3297
XmiI GTMKAC 3 cut(s) 688, 2425, 2773
XmnI GAANNNNTTC 1 cut(s) 3159
XspI CTAG 8 cut(s) 344, 416, 1242, 1431, 1775, 3129, 3321, 3572
ZrmI AGTACT 1 cut(s) 852
Zsp2I ATGCAT 1 cut(s) 1838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.