Rroxscaffold_7G00165160

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001838
Physical Location & Seq
Forward (+)
7027381 .. 7033140
5760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00165160.1

Sequence Viewer

Length: 4443 bp
ATGGCTAAGAACTATAGATTTTCAATGGACCAAAAGGACATTGTTAGGGTATTGATTACCACAGTAGATGGTTTTATTCGGGATCAGCTGATCAATAAAGAACAGAGAAGCCAACACAGGGAACAGTGTGCTGAAAGATTGGCAGCTGAAGATGGATCCTGTGGTAAAGAAACCGAGGTTCGTTACTCTGATCAAGCAGTGCTAGCAAATCTGGATTGGGGTATTGAGGCTCTTGAAGAAGCAATCGACACTTCAAATATGGAAACTAAGCTTGCAAGACTAGACCATGCTGAAAAAATGTTGCAAGTTTGTGCCATGTTGAATTCTGATCAGAAAACTGCTGGAGTCCCCAATTTTTACCTCTCAGCTTGGGCACACCTCAACTTAGCATACCTGTGGAAATTGAGGAATAACATCCAAAACTCAGTTCTCCATGTCATTGAGATGTTTACAGTTGATCCTTTTTTCTCACGGATAGATTTTGCTCCTGAAATCTGGAAACTTTTGTTTCTTCCACACATGAGCTCAATTATAGGGTGGTATTCGGAGCAGAGGCACCGGCTTGTGATGGAAGTAATTCCTGATTCTCATGATCTGTCTTTCACAGCTGATCTTGATCAATTTTTCAATGAGTCTTTAATCTTTTCAATGAGGCCGGATCAGATAGAGAAACTGCAAAAGCTGGAGCAGCTTTATGGTGAGTCACTGGATGAGAACACAAGGCTTTATGCCAAGTACTTCAAGGATTGCATGACATCTGATTCAACCTCCAGTAAGAAAGTGATTCCAATGTTGCCAATTGCTGAGCCACCAATGACTCCATTGCATGAGGTCAGCCGCTCGATTCCTGATTTTGTCAAGTTTGGTCCTATCTTGCCCAAGAGTGCTGGCTTTTCTCCCATCCTCAAGTCTAAAGATGGCACAAGAGACATAAACAGAATGAATCTAAGTTCTGCATCTTCACAAAATTTGGAGCCTGTCAGATGGGATCCTCAGGAATGTATCCCCGAGGAGAACGAATATGAATCCGATTATGAGCCAAATGATGCTAATATGGCTTCTGATCACGAGAAGGAAACTGGAGGAAAAGCACAGTCATCTGTACTCAGGAGTCGAGTACATAGCCCAACAATCTTTTCTCCACTAATTTCTCCTAAAACTTCACCAAAAGTTTCATCTCCCAAACCAGACACAGCTGCTTCTGCATTGCGTTTACTGTCTGTCAAAATCACAGATTCAGCCGTTGCCACCTCTTTGCCTGCATCTCCGGGCATGAGCAATGATTACAGCATCAGCTCTGCAGACTCTGATGTTGAAGTGATAGAGACTACCAAAAGTTGCAGGAAAGTCTATAGCCGAACATCAAGCATGAATAATGAGCTTGTGAAAATGTCGAAAAATAGTCCACCTAATGAAAATGATGACGGAGGCCAGAGCTGTGTTTCACTTCCATCTTCTGAGAGGATGACTACTAAGTCAAGATCACCTAAGGATTTTGTTTGTCCTATCACTGGCCAGATATTTATTGATCCAGTTACTCTTGAAACAGGTCAGACATACGAAAGAAAAGCAATCCAAGAATGGCTGAAACGAGGAAACACAACCTGTCCCATTACGCGGCAACCTATCTCTACAACTACTCAGCTGCCTAAAACAAACTATGTTTTGAAAAGACTGATAACCTCTTGGAAAGAACAGCATCCTGAACTCGCTCAAGAATGTGCATACTATGAAACACCAAGAAACTCATTCCACCACTCCTCCGTGAAAGAAGTCCATTCTGGTACCACAACTCCACAAAGAACATGTGACTTCATGGGTCATAGGAATACCGATGATTATATCAGCCAAAGGAGCAAAAGGTTTATGCAGGCAGCTGTTTCTACATCACCAACCAGTGTGATATCTCAAGCTGCAGTTGAGACAATTATCAATGGCTTAAAGCCTCATGTTGCATGTCTGTGTACTTCAGATCAACTTCAAGAGTGTGAAACAGCAGTGTTAGAAATAGCCAGATTATGGAAGGACTCAAAGAGTGATCCTGCAATTCATACATTCTTATCGGAGCTGACAACTGTGAATGGATTCATCGAAATACTTTCAGCTTCTCTGAATAGGGAAGTCCTGAGAACATCTATTTACATCCTCTCAGAGCTAATATTTGCCGATGAAAGTGTTGGAGAAACACTTACCAGTGTAGACTCCGACCTTGATTGCCTGGCTGCTCTTTTAAAGAATGGACTAGCTGAGCCTGCTGTTCTCATTTACCAGCTGAGGCCAGTGTTTGCTCAGCTTTCAGCTCATGATCTTATACCCTCCCTTGTGCAGCTAATTCAGAGCAAAAATGAGGAATCAAATGATCTTCAATTAGTAATGAACCCCAAAGATGCTGCATTAGCTATTCTTGAGCAAGTCTTGATGGGAGGAGATGAAAATAGCAGATCCATTAATGCTTTGAGTGTTTTTTCTGCGAGTGGTATACCTGCTCTAGTTAAGTGTTTAGATAGGCCAGAAGGAAGAAGGTCCATTGTTTCTATTCTATTATGCTGCATGCAAGCTGAAAAAACTTGCAGGAACTTGATAGCAAATAAAATTGATCTGTCTCCTGTTCTCGAACTATTTCACGCTGGAAGTGATGGTGTGAGAGGCATATGTGTAGAGTTTCTCTCAGAACTGGTACAGTTGAACAGGAGAACATTATGCAATCAGATCTTGCAGACGATTAAAGATGAAGGAGCATTTAGTACAATGCATACCTTTCTTGTATATCTTCAAATGGCTCCAATGGAGCAGCAACCTGCAATTGCTACTCTCCTTCTTCAGCTTGATCTCCTGGTTTCCCTCTTTCCTGTTGAGCCTTGGAAGATGAGCATTTATAGGGAAGAATCTATAGAGGGACTAATTGAGGCACTCCGAAGGAAAGACTTCTCAAACTCTCAAATGATGGCTTTAGATGCCTTGTTATCTCTTACTGGACGTGTGACTTCATCCGGGGATTCATACACTGAAGCCTGGTTACTCAAGATTGCTGGGTTTGATCAACCTTACAATGCTTTGATGAAGGCAGAGCGGCTAAGGAAACAAGACAATGATTTGATGGAGACAATGGAAGAAGAAGAGAAAGCAGTAAGCTCTTGGCAGAAAAAAGTTGCTTTTGTACTTTGCAACCATGAAAGGGGATCCATTTTTAAAGCTTTGGGAGAATGCCTCAAGAGTAATTCCATTGAGATGGCAAAGTCATGTCTTGTCATAGCTACATGGCTCACACACATGCTTTCTGTACTTCCTGATACAGGTGTGAAAATCAGTGCTCGCAAGGCCTTGCTTGAAGAATTCATAAATGTTCTCCAATCATCGAATAATTTGGAGGAGAAGATTTTAGCAACCCTGGCTTTGAAATCTTTTGTCAGTGAACCAGCTGCACTTGAAGCACTGGGAGTGTATGCTAAATGCATCTACAAAACATTGAGAAAGCTTAAGAGGAGCACTGTGGTGGCGAGTGACATAATGAAAGCGCTGATGAACTTGTCATCTGTTGACGTAACAGAGTTGTGGAGCTGTGCTGAAGTGGTTGAACTAGATTCAAGCTCAAATGGGGAGGTTACATCTATACTTCATCTAAAAGGCCGTGTTTTAAGCAGCCATTCTGATGGAACTATGAAGGTATGGGATGCTGGCAAGAAAGTATTAAGACTGATTCAAGAAGTCCGCGAGCACACAAAAGCTGTCACATGCCTCTATATTTCACCTTCAGGTGATAAATTATACAGCGGTTCGTTAGACAAAACAATTCGGGTGTGGGCAATCAAAGCAGAAGAAATTCATTGCCTTCAGGTTCACGATGTGAAAGAGGTAGTATATGAGTTGGTAGCGAATGCTAAAGTGGCGTGCTTCATTTCTCAAGGCACTGGAGTGAAGGTTTATGACTGGTCTGGGGTCCAAAAGCATGTAAACTTCAACAAATATGTAAAGAGCCTAGCCATGTCAGGGAACAGCCTCTATTGTGGTTGCTCTGGTTACAGCATCCAGGAGATCGACTTGGGGAGACATATACCAAGCACATTCTACTCCGGCACAAGAAAGCTGTTGGGAAAACAGATGTTCTATTCCCTTGAGATTCATGATGGCATCCTCTATGCGGGCGGTTCATCAGTTGATGCAACAGCTGGGAAGATATTTTCGCTTCCAAACAAGGCGGTATTAGGATCATTCTCAACCGGATTTGACATCCAACGCATTGCCACCAACAATGACTTGATCTTTACGGCAACAAAGTGTGGGATAATTGAGGTTTGGTTGAAAGAGAGGTTCACAAGAATTGCTTCCATCAAAACAGCGGGCGGCGGTCATGCCAAGATCACATCCTTGGCGGCAGATATGGACGGAGGATTGCTGTTTGCAGGTTCCTCTGATGGCAGAATCCAGGTTTGGGCACTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1480

Amino Acids

164.53

Weight (kDa)

5.78

Isoelectric Point (pI)

47.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 9 - 268 1.9e-75 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 496 - 567 1.5e-15 U-box domain
ARM_LIN_2nd PF23654 642 - 803 1e-61 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 804 - 1171 3.6e-113 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR3_1st PF25173 1189 - 1331 1.4e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 1197 - 1273 7.3e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 1201 - 1294 4.8e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 1205 - 1314 4.9e-10 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1208 - 1279 8e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1208 - 1299 3.6e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 1210 - 1272 2.9e-08 WDR3 second beta-propeller domain
WD40 PF00400 1232 - 1267 5.3e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1474
Acc36I ACCTGC 3 cut(s) 2491, 2806, 4391
Acc65I GGTACC 1 cut(s) 1784
AccB1I GGYRCC 2 cut(s) 555, 1784
AccB7I CCANNNNNTGG 1 cut(s) 2019
AccBSI CCGCTC 2 cut(s) 840, 3070
AccI GTMKAC 2 cut(s) 2199, 2479
AccII CGCG 2 cut(s) 1618, 3711
AcoI YGGCCR 1 cut(s) 1513
AcsI RAATTY 4 cut(s) 322, 967, 3332, 3819
AcuI CTGAAG 7 cut(s) 168, 1953, 2804, 3027, 3585, 3735, 3815
AdeI CACNNNGTG 1 cut(s) 3844
AfaI GTAC 9 cut(s) 737, 1104, 1119, 1786, 1966, 2679, 2746, 3159, 3282
AfeI AGCGCT 1 cut(s) 3516
AfiI CCNNNNNNNGG 9 cut(s) 118, 1511, 1617, 2019, 3175, 3293, 3987, 4138, 4429
AflII CTTAAG 1 cut(s) 3476
AflIII ACRYGT 2 cut(s) 1805, 2977
AjiI CACGTC 1 cut(s) 2978
AjnI CCWGG 6 cut(s) 2217, 2832, 3011, 3387, 4026, 4422
AjuI GAANNNNNNNTTGG 6 cut(s) 411, 443, 1120, 1152, 4411, 4443
AleI CACNNNNGTG 2 cut(s) 3491, 3911
Alw21I GWGCWC 4 cut(s) 527, 3313, 3488, 3717
Alw26I GTCTC 6 cut(s) 921, 1319, 1916, 2607, 3095, 4039
AlwNI CAGNNNCTG 1 cut(s) 1307
Ama87I CYCGRG 1 cut(s) 1007
Aor51HI AGCGCT 1 cut(s) 3516
AoxI GGCC 7 cut(s) 653, 1429, 1513, 2276, 2507, 3318, 3625
ApoI RAATTY 4 cut(s) 322, 967, 3332, 3819
AseI ATTAAT 1 cut(s) 2448
Asp700I GAANNNNTTC 6 cut(s) 576, 2084, 2619, 2924, 3819, 4321
Asp718I GGTACC 1 cut(s) 1784
AspLEI GCGC 1 cut(s) 3517
AspS9I GGNCC 4 cut(s) 28, 866, 2523, 3937
AsuC2I CCSGG 2 cut(s) 1269, 2992
AsuHPI GGTGA 6 cut(s) 710, 1155, 1476, 1881, 3738, 3767
AsuNHI GCTAGC 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 1007
AvaII GGWCC 4 cut(s) 28, 866, 2523, 3937
AxyI CCTNAGG 2 cut(s) 993, 1488
BaeGI GKGCMC 2 cut(s) 376, 4435
BaeI ACNNNNGTAYC 2 cut(s) 3282, 3315
BalI TGGCCA 1 cut(s) 1515
BamHI GGATCC 3 cut(s) 155, 988, 3179
BanI GGYRCC 2 cut(s) 555, 1784
BanII GRGCYC 1 cut(s) 527
BarI GAAGNNNNNNTAC 2 cut(s) 3000, 3032
BauI CACGAG 1 cut(s) 1067
Bbv12I GWGCWC 4 cut(s) 527, 3313, 3488, 3717
BbvCI CCTCAGC 1 cut(s) 2273
BceAI ACGGC 3 cut(s) 1226, 3612, 4281
BciT130I CCWGG 6 cut(s) 2219, 2834, 3013, 3389, 4028, 4424
BciVI GTATCC 1 cut(s) 1013
BclI TGATCA 6 cut(s) 90, 190, 328, 616, 1063, 3037
BcnI CCSGG 2 cut(s) 1269, 2992
BcoDI GTCTC 6 cut(s) 921, 1319, 1916, 2607, 3095, 4039
BfaI CTAG 7 cut(s) 203, 281, 2243, 2489, 3578, 3977, 4436
BfmI CTRYAG 5 cut(s) 13, 1299, 1351, 1914, 2889
BfoI RGCGCY 1 cut(s) 3518
BfrI CTTAAG 1 cut(s) 3476
BfuAI ACCTGC 3 cut(s) 2491, 2806, 4391
BfuI GTATCC 1 cut(s) 1013
BglII AGATCT 1 cut(s) 2709
BlpI GCTNAGC 3 cut(s) 804, 2247, 2289
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 8 cut(s) 1269, 2219, 2834, 2992, 3013, 3389, 4028, 4424
Bme18I GGWCC 4 cut(s) 28, 866, 2523, 3937
BmeT110I CYCGRG 1 cut(s) 1007
BmgBI CACGTC 1 cut(s) 2978
BmgT120I GGNCC 4 cut(s) 28, 866, 2523, 3937
BmiI GGNNCC 9 cut(s) 157, 557, 975, 990, 1786, 2781, 3181, 3938, 4405
BmrFI CCNGG 8 cut(s) 1269, 2219, 2834, 2992, 3013, 3389, 4028, 4424
BmrI ACTGGG 1 cut(s) 3443
BmtI GCTAGC 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 3443
BplI GAGNNNNNCTC 4 cut(s) 2651, 2683, 3192, 3224
BpmI CTGGAG 5 cut(s) 363, 704, 754, 1101, 3930
Bpu10I CCTNAGC 2 cut(s) 2273, 3074
Bpu1102I GCTNAGC 3 cut(s) 804, 2247, 2289
BpuEI CTTGAG 8 cut(s) 890, 1698, 1893, 2426, 3005, 3194, 3885, 4133
BpuMI CCSGG 2 cut(s) 1269, 2992
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 6 cut(s) 174, 1008, 2858, 2991, 3387, 4365
BsaWI WCCGGW 1 cut(s) 4217
BsaXI ACNNNNNCTCC 6 cut(s) 1745, 1775, 1777, 1807, 3903, 3933
Bsc4I CCNNNNNNNGG 9 cut(s) 118, 1511, 1617, 2019, 3175, 3293, 3987, 4138, 4429
Bse118I RCCGGY 1 cut(s) 558
Bse21I CCTNAGG 2 cut(s) 993, 1488
Bse3DI GCAATG 5 cut(s) 821, 1205, 1285, 3823, 4236
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 6 cut(s) 2219, 2834, 3013, 3389, 4028, 4424
BseDI CCNNGG 6 cut(s) 174, 1008, 2858, 2991, 3387, 4365
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 9 cut(s) 118, 1511, 1617, 2019, 3175, 3293, 3987, 4138, 4429
BseMI GCAATG 5 cut(s) 821, 1205, 1285, 3823, 4236
BseRI GAGGAG 5 cut(s) 1025, 1750, 2439, 3383, 3496
BseSI GKGCMC 2 cut(s) 376, 4435
BseYI CCCAGC 2 cut(s) 3029, 4166
BsgI GTGCAG 2 cut(s) 2345, 3405
Bsh1236I CGCG 2 cut(s) 1618, 3711
BshFI GGCC 7 cut(s) 655, 1431, 1515, 2278, 2509, 3320, 3627
BshNI GGYRCC 2 cut(s) 555, 1784
BsiHKAI GWGCWC 4 cut(s) 527, 3313, 3488, 3717
BsiHKCI CYCGRG 1 cut(s) 1007
BsiSI CCGG 6 cut(s) 559, 656, 1268, 2991, 4071, 4218
BslFI GGGAC 3 cut(s) 332, 1593, 2910
BslI CCNNNNNNNGG 9 cut(s) 118, 1511, 1617, 2019, 3175, 3293, 3987, 4138, 4429
BsmAI GTCTC 6 cut(s) 921, 1319, 1916, 2607, 3095, 4039
BsmFI GGGAC 3 cut(s) 332, 1593, 2910
BsmI GAATGC 2 cut(s) 3209, 3880
BsnI GGCC 7 cut(s) 655, 1431, 1515, 2278, 2509, 3320, 3627
BsoBI CYCGRG 1 cut(s) 1007
Bsp1286I GDGCHC 6 cut(s) 376, 527, 3313, 3488, 3717, 4435
Bsp1720I GCTNAGC 3 cut(s) 804, 2247, 2289
BspANI GGCC 7 cut(s) 655, 1431, 1515, 2278, 2509, 3320, 3627
BspFNI CGCG 2 cut(s) 1618, 3711
BspHI TCATGA 3 cut(s) 589, 2302, 4120
BspLI GGNNCC 9 cut(s) 157, 557, 975, 990, 1786, 2781, 3181, 3938, 4405
BspMAI CTGCAG 2 cut(s) 1303, 1918
BspMI ACCTGC 3 cut(s) 2491, 2806, 4391
BspOI GCTAGC 1 cut(s) 206
BspT107I GGYRCC 2 cut(s) 555, 1784
BspTI CTTAAG 1 cut(s) 3476
BsrBI CCGCTC 2 cut(s) 840, 3070
BsrDI GCAATG 5 cut(s) 821, 1205, 1285, 3823, 4236
BsrFI RCCGGY 1 cut(s) 558
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 6 cut(s) 174, 1008, 2858, 2991, 3387, 4365
BssNAI GTATAC 1 cut(s) 2480
BssSI CACGAG 1 cut(s) 1067
BssT1I CCWWGG 2 cut(s) 2858, 4365
Bst1107I GTATAC 1 cut(s) 2480
Bst2BI CACGAG 1 cut(s) 1067
Bst2UI CCWGG 6 cut(s) 2219, 2834, 3013, 3389, 4028, 4424
Bst4CI ACNGT 8 cut(s) 64, 126, 454, 1095, 1218, 2077, 2682, 3490
Bst6I CTCTTC 1 cut(s) 3111
BstAFI CTTAAG 1 cut(s) 3476
BstENI CCTNNNNNAGG 1 cut(s) 3291
BstFNI CGCG 2 cut(s) 1618, 3711
BstH2I RGCGCY 1 cut(s) 3518
BstHHI GCGC 1 cut(s) 3517
BstMAI GTCTC 6 cut(s) 921, 1319, 1916, 2607, 3095, 4039
BstNI CCWGG 6 cut(s) 2219, 2834, 3013, 3389, 4028, 4424
BstNSI RCATGY 6 cut(s) 1809, 1959, 2554, 3274, 3735, 3950
BstSCI CCNGG 8 cut(s) 1267, 2217, 2832, 2990, 3011, 3387, 4026, 4422
BstSFI CTRYAG 5 cut(s) 13, 1299, 1351, 1914, 2889
BstSLI GKGCMC 2 cut(s) 376, 4435
BstUI CGCG 2 cut(s) 1618, 3711
BstX2I RGATCY 5 cut(s) 155, 988, 2441, 2709, 3179
BstXI CCANNNNNNTGG 2 cut(s) 3229, 3650
BstYI RGATCY 5 cut(s) 155, 988, 2441, 2709, 3179
BstZ17I GTATAC 1 cut(s) 2480
Bsu36I CCTNAGG 2 cut(s) 993, 1488
BsuI GTATCC 1 cut(s) 1013
BsuRI GGCC 7 cut(s) 655, 1431, 1515, 2278, 2509, 3320, 3627
BtrI CACGTC 1 cut(s) 2978
BtsI GCAGTG 2 cut(s) 204, 2004
BveI ACCTGC 3 cut(s) 2491, 2806, 4391
CaiI CAGNNNCTG 1 cut(s) 1307
CciI TCATGA 3 cut(s) 589, 2302, 4120
CfoI GCGC 1 cut(s) 3517
Cfr10I RCCGGY 1 cut(s) 558
Cfr13I GGNCC 4 cut(s) 28, 866, 2523, 3937
Csp6I GTAC 9 cut(s) 736, 1103, 1118, 1785, 1965, 2678, 2745, 3158, 3281
CviQI GTAC 9 cut(s) 736, 1103, 1118, 1785, 1965, 2678, 2745, 3158, 3281
DraI TTTAAA 2 cut(s) 2232, 3190
DraIII CACNNNGTG 1 cut(s) 3844
DrdI GACNNNNNNGTC 1 cut(s) 1474
DseDI GACNNNNNNGTC 1 cut(s) 1474
EaeI YGGCCR 1 cut(s) 1513
Eam1104I CTCTTC 1 cut(s) 3111
EarI CTCTTC 1 cut(s) 3111
Ecl136II GAGCTC 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 2858, 4365
Eco147I AGGCCT 1 cut(s) 3320
Eco24I GRGCYC 1 cut(s) 527
Eco32I GATATC 1 cut(s) 1905
Eco47I GGWCC 4 cut(s) 28, 866, 2523, 3937
Eco47III AGCGCT 1 cut(s) 3516
Eco53kI GAGCTC 1 cut(s) 525
Eco57I CTGAAG 7 cut(s) 168, 1953, 2804, 3027, 3585, 3735, 3815
Eco81I CCTNAGG 2 cut(s) 993, 1488
Eco88I CYCGRG 1 cut(s) 1007
EcoICRI GAGCTC 1 cut(s) 525
EcoNI CCTNNNNNAGG 1 cut(s) 3291
EcoRI GAATTC 2 cut(s) 322, 3332
EcoRII CCWGG 6 cut(s) 2217, 2832, 3011, 3387, 4026, 4422
EcoRV GATATC 1 cut(s) 1905
EcoT14I CCWWGG 2 cut(s) 2858, 4365
EcoT22I ATGCAT 2 cut(s) 2754, 3455
EcoT38I GRGCYC 1 cut(s) 527
ErhI CCWWGG 2 cut(s) 2858, 4365
FalI AAGNNNNNCTT 2 cut(s) 4306, 4338
FaqI GGGAC 3 cut(s) 332, 1593, 2910
FauI CCCGC 2 cut(s) 4132, 4330
FauNDI CATATG 1 cut(s) 2651
FbaI TGATCA 6 cut(s) 90, 190, 328, 616, 1063, 3037
FblI GTMKAC 2 cut(s) 2199, 2479
FriOI GRGCYC 1 cut(s) 527
FspBI CTAG 7 cut(s) 203, 281, 2243, 2489, 3578, 3977, 4436
GlaI GCGC 1 cut(s) 3516
GsaI CCCAGC 2 cut(s) 3033, 4170
GsuI CTGGAG 5 cut(s) 363, 704, 754, 1101, 3930
HaeII RGCGCY 1 cut(s) 3518
HaeIII GGCC 7 cut(s) 655, 1431, 1515, 2278, 2509, 3320, 3627
HapII CCGG 6 cut(s) 559, 656, 1268, 2991, 4071, 4218
HhaI GCGC 1 cut(s) 3517
Hin6I GCGC 1 cut(s) 3515
HinP1I GCGC 1 cut(s) 3515
HincII GTYRAC 1 cut(s) 3538
HindII GTYRAC 1 cut(s) 3538
HindIII AAGCTT 3 cut(s) 269, 3192, 3473
HpaII CCGG 6 cut(s) 559, 656, 1268, 2991, 4071, 4218
HphI GGTGA 6 cut(s) 710, 1155, 1476, 1881, 3738, 3767
HpyCH4III ACNGT 8 cut(s) 64, 126, 454, 1095, 1218, 2077, 2682, 3490
HpyCH4IV ACGT 2 cut(s) 2977, 3540
HpySE526I ACGT 2 cut(s) 2977, 3540
HspAI GCGC 1 cut(s) 3515
KpnI GGTACC 1 cut(s) 1788
Ksp22I TGATCA 6 cut(s) 90, 190, 328, 616, 1063, 3037
MaeI CTAG 7 cut(s) 203, 281, 2243, 2489, 3578, 3977, 4436
MaeII ACGT 2 cut(s) 2977, 3540
MbiI CCGCTC 2 cut(s) 840, 3070
MfeI CAATTG 2 cut(s) 798, 2802
MflI RGATCY 5 cut(s) 155, 988, 2441, 2709, 3179
MhlI GDGCHC 6 cut(s) 376, 527, 3313, 3488, 3717, 4435
MlsI TGGCCA 1 cut(s) 1515
MluNI TGGCCA 1 cut(s) 1515
MlyI GAGTC 8 cut(s) 354, 641, 710, 811, 1120, 1298, 2021, 2195
MmeI TCCRAC 3 cut(s) 2158, 2229, 4255
Mox20I TGGCCA 1 cut(s) 1515
Mph1103I ATGCAT 2 cut(s) 2754, 3455
MroXI GAANNNNTTC 6 cut(s) 576, 2084, 2619, 2924, 3819, 4321
MscI TGGCCA 1 cut(s) 1515
MslI CAYNNNNRTG 8 cut(s) 394, 443, 1960, 3227, 3269, 3491, 3648, 3911
Msp20I TGGCCA 1 cut(s) 1515
MspCI CTTAAG 1 cut(s) 3476
MspI CCGG 6 cut(s) 559, 656, 1268, 2991, 4071, 4218
MspR9I CCNGG 8 cut(s) 1269, 2219, 2834, 2992, 3013, 3389, 4028, 4424
MunI CAATTG 2 cut(s) 798, 2802
Mva1269I GAATGC 2 cut(s) 3209, 3880
MvaI CCWGG 6 cut(s) 2219, 2834, 3013, 3389, 4028, 4424
MvnI CGCG 2 cut(s) 1618, 3711
NciI CCSGG 2 cut(s) 1269, 2992
NdeI CATATG 1 cut(s) 2651
NheI GCTAGC 1 cut(s) 202
NlaIV GGNNCC 9 cut(s) 157, 557, 975, 990, 1786, 2781, 3181, 3938, 4405
NmuCI GTSAC 5 cut(s) 702, 1808, 2980, 3500, 3727
NsiI ATGCAT 2 cut(s) 2754, 3455
NspI RCATGY 6 cut(s) 1809, 1959, 2554, 3274, 3735, 3950
OliI CACNNNNGTG 2 cut(s) 3491, 3911
PaeI GCATGC 1 cut(s) 2554
PagI TCATGA 3 cut(s) 589, 2302, 4120
PceI AGGCCT 1 cut(s) 3320
PciI ACATGT 1 cut(s) 1805
PctI GAATGC 2 cut(s) 3209, 3880
PdmI GAANNNNTTC 6 cut(s) 576, 2084, 2619, 2924, 3819, 4321
PflMI CCANNNNNTGG 1 cut(s) 2019
PfoI TCCNGGA 1 cut(s) 4026
PleI GAGTC 8 cut(s) 353, 640, 709, 811, 1119, 1298, 2021, 2195
PpsI GAGTC 8 cut(s) 353, 640, 709, 811, 1119, 1298, 2021, 2195
PscI ACATGT 1 cut(s) 1805
PshBI ATTAAT 1 cut(s) 2448
Psp124BI GAGCTC 1 cut(s) 527
Psp6I CCWGG 6 cut(s) 2217, 2832, 3011, 3387, 4026, 4422
PspFI CCCAGC 2 cut(s) 3029, 4166
PspGI CCWGG 6 cut(s) 2217, 2832, 3011, 3387, 4026, 4422
PspN4I GGNNCC 9 cut(s) 157, 557, 975, 990, 1786, 2781, 3181, 3938, 4405
PspPI GGNCC 4 cut(s) 28, 866, 2523, 3937
PstI CTGCAG 2 cut(s) 1303, 1918
PstNI CAGNNNCTG 1 cut(s) 1307
PsuI RGATCY 5 cut(s) 155, 988, 2441, 2709, 3179
PvuII CAGCTG 9 cut(s) 88, 146, 608, 1196, 1645, 1877, 2272, 3419, 4166
RsaI GTAC 9 cut(s) 737, 1104, 1119, 1786, 1966, 2679, 2746, 3159, 3282
RsaNI GTAC 9 cut(s) 736, 1103, 1118, 1785, 1965, 2678, 2745, 3158, 3281
RseI CAYNNNNRTG 8 cut(s) 394, 443, 1960, 3227, 3269, 3491, 3648, 3911
SacI GAGCTC 1 cut(s) 527
Sau96I GGNCC 4 cut(s) 28, 866, 2523, 3937
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 8 cut(s) 354, 641, 710, 811, 1120, 1298, 2021, 2195
ScrFI CCNGG 8 cut(s) 1269, 2219, 2834, 2992, 3013, 3389, 4028, 4424
SduI GDGCHC 6 cut(s) 376, 527, 3313, 3488, 3717, 4435
SfcI CTRYAG 5 cut(s) 13, 1299, 1351, 1914, 2889
SinI GGWCC 4 cut(s) 28, 866, 2523, 3937
SmiMI CAYNNNNRTG 8 cut(s) 394, 443, 1960, 3227, 3269, 3491, 3648, 3911
SmlI CTYRAG 9 cut(s) 905, 1713, 1908, 2405, 3020, 3209, 3476, 3900, 4112
SmoI CTYRAG 9 cut(s) 905, 1713, 1908, 2405, 3020, 3209, 3476, 3900, 4112
SphI GCATGC 1 cut(s) 2554
SseBI AGGCCT 1 cut(s) 3320
SspI AATATT 1 cut(s) 2160
SspMI CTAG 7 cut(s) 203, 281, 2243, 2489, 3578, 3977, 4436
SstI GAGCTC 1 cut(s) 527
StuI AGGCCT 1 cut(s) 3320
StyD4I CCNGG 8 cut(s) 1267, 2217, 2832, 2990, 3011, 3387, 4026, 4422
StyI CCWWGG 2 cut(s) 2858, 4365
TaaI ACNGT 8 cut(s) 64, 126, 454, 1095, 1218, 2077, 2682, 3490
TaiI ACGT 2 cut(s) 2980, 3543
TaqI TCGA 8 cut(s) 246, 842, 1114, 1394, 2091, 2613, 3356, 4035
TatI WGTACW 7 cut(s) 735, 1102, 1117, 1964, 2744, 3157, 3280
TauI GCSGC 5 cut(s) 840, 1621, 3073, 4344, 4373
TseFI GTSAC 5 cut(s) 702, 1808, 2980, 3500, 3727
Tsp45I GTSAC 5 cut(s) 702, 1808, 2980, 3500, 3727
TspGWI ACGGA 4 cut(s) 487, 1440, 1753, 4398
Van91I CCANNNNNTGG 1 cut(s) 2019
Vha464I CTTAAG 1 cut(s) 3476
VpaK11BI GGWCC 4 cut(s) 28, 866, 2523, 3937
VspI ATTAAT 1 cut(s) 2448
XagI CCTNNNNNAGG 1 cut(s) 3291
XapI RAATTY 4 cut(s) 322, 967, 3332, 3819
XceI RCATGY 6 cut(s) 1809, 1959, 2554, 3274, 3735, 3950
XmiI GTMKAC 2 cut(s) 2199, 2479
XmnI GAANNNNTTC 6 cut(s) 576, 2084, 2619, 2924, 3819, 4321
XspI CTAG 7 cut(s) 203, 281, 2243, 2489, 3578, 3977, 4436
ZrmI AGTACT 1 cut(s) 737
Zsp2I ATGCAT 2 cut(s) 2754, 3455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.