Rh2AG060300

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001838
Physical Location & Seq
Reverse (-)
4862368 .. 4866855
4488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG060300.1

Sequence Viewer

Length: 2943 bp
ATGGAATTATTTGATTCTAGGCGGAACCAAATCCAAAAGCTAAAGCAACCCATTTCCGCAGAATCAAGTCGTTCATCTCCAAATCCGCTCATGGCAGATTCTGAAAACCAATCTGGCGGTGGAAAATTTACACCTCCTAAGGACTTTGTGTGTCCCATAACTAGTACCCTTTTTGATGATCCAGTGACACTTGAGACTGGTCAGACATATGAGCGAAAAGCCATACAAGAATGGCTTGATAGAGGAAACTCAACCTGCCCAATTACCCGCCAGAATTTGCAAAGCACCCAACTGCCTAAGACCAACTATGTCCTCAAGCGTCTAATTGCAAGTTGGCAAGAACAGAACCCTGGTATAGCCTTCAGCTCATCTGATAATCCAAGCCCGCAGATTGAGCCAATGGTGAAACCCATAATGCCTTCAACTTCTCCTGTCAGTGTCATAAGCCAAGCTGCTATTGATAGTGCAGTTAGTGAGTTAAGAATTGCCATTACCAATCTTTGTATGTCAGAAATTCTCAAGGAGTCTGAAATGGCTGTTCTTCGTATTGAGCGTTTCTGGCAGGAAGCAAATGTGGAATGGGATATTCAGAATATGCTCTCAAAACCTCCGGTTATTAATGGTTTCGTGGAGGTTCTTTTCAATTCGGTCGATCCTAGAGTTCTAACAGCTACAGTTTTCCTCTTGTCTGAGCTGGGTTCAAGAGACAATGCTGTGATTCAGACACTAACCCAGGTTGACTCTGATGTGGAATGTATTGTGGCTCTTTTCAAAAAGGGGTTGATGGAGGCTGTTGTCTTGATATATCTGCTAAGGCATTGCATTCTTAATCTAGTAGAGATGGACCTGGTGGACTCTCTCTTAATGGTTATTAGAAAGAGAGATGATGATTTGGCTAATATGTGTCTAAAGCCTAAATCAGCTGCAGTGGTTTTACTGGGGCATATTGTTGGTGGCAGTGAATCAATCGCCAACACCGTGACTTCTGAAAAAGCACTTGAAAGAATAATCAGCAGTTTGGAATCTGAATCAGCGGAAGAGAAGGCTGCAGCGATTGGGATCTTATTAAGATGTATGCAGCAGGATGGGCACTGCAGGAACACAATAGCTGATAAAGCTGAGTTGGCTCCTGTTTTGGATAGCTTCACGGGTGCAAAGGATGAAGAACGGTTTGAGATAGTTCTCTTTTTGTCCGAGTTAGTGAAATTAAACAGGAGGACATTCAATGAACAAATCCTGCATATCATAAAGGATGAGGGCCCTTTCAGTACAATGCACACCCTCCTTATTTATCTACAGACTGCTGCTCAAGACCATTGTCCTGTTGTGGCCAGTCTACTACTTCAACTTGATCTTCTGGCAGAGCCGAGAAAGATGAGCATATACCGTGAAGAGGCAATGGACACTCTCATTTCATGTCTCAGAAATATTGAGGCCCCTGCTGCCCAAATCGCTGCTGCTGAGACCATTATGTCACTGCAAGGGACGTTCACCACATCCGGAAAGCCTCTTGCTAGGGCCGTCCTTCTAAAACGTGCTGGCCTTGACAAAATTTATAAACAACGTGTGCGAATGGATCAACTTAGCAACTTTTCTGGAGAAGTTGAAACTGTTGAGGAAGAAAAGGCAGCTGATAACTGGGAAAGAAAAATGGCATTTGTTTTAGCAAGCCACGAGTTTGGTCTACTTTTTGAGGCCTTGGCAGAAGGATTGAAGAGCAGAATCTCGGAATTATGTTCTGCATGCTTTGTGTCAGCCACATGGCTCGTACACATGCTCAATGCCCTTCCAGATACAGGAATACGAGGAGCAGCTCGGCTGTGCTTGATTAAACGGTTCATATCAATATTCAAGTCTGCCAAGGATACTGAAGACAAAGCACTTGCTTTGCTGGCGTTGGGCAGCTTCATCCGTGATCCTGAGGGGATGCAAGAATTAAATTCTTGCAGGAGGGATATCGTAAAGGGCCTGAGAGAGCTTAAGAGATACACTCCCTTGGCATTTCAAATGCTAAAAGTCTTTTCCGATGGGCAAGACTCTAGTGCCGAATTGTGGGATCATAAAGAATTAGTTCAAGTAGACTGCAGTGAGAACGGAGAGGTTCTTTCAGTAGTTTGTTTCAAGGATAAAATATTTTCTGGCCATTCAGATGGAACTATAAAGGTGTGGACGGGTAAAGGTAGCATTCTCCATCTAATCCAGGAAATTCAAGAACATACAAAGGCAGTTACTAGCTTGGCAATTTTGCAATCAAAGGAGATTCTATACAGTGGCTCACTAGATAGAACAACAAGGGTCTGGTCTATCAGCAATGAAGGAATCCAGTGTGTAGAAGTCCATGATATGAAGGATCAAGTTCATAATCTGGCCGTCACCAATAGTTTTTCATGCTTCATTCCACAAAGCAATGGTATAAAGGCTCACTCATGGAGCGGAGGATCCAAAATGTTAAATTCCAGCAAATCTGTAAAGTGCTTTGCTCTGGTAAATGGGAAAATATATTGTGGATGCCATGATAGCAGTGTTCAGGAGATTGATTTGGTCACAGGAACACTCAGTACCATTCAAACCGGCTCCCGGAAATTACTTGGGAAATCAAATCCTATCCATTGCATTCAGTATCACAGTGGAATGATATATGCAGCAGGTTCTTCGTTGGATGGAGCAGCAGTGAAGATTTGGAGTGCTGCAAACTTCAGTTTGGTTGGGTCTCTGTCAACTACACTAGAAGTGCGGGCTATGGCAATAAGCTCGGAACTGATCTATTTGGGAGGTAAAGGAGGATGTGTGGAAATTTGGGATAGGGAGAAGCAGAACAAAATAATAGACACACTACAAACTGGCACAAACTGTAAGGTTCTTTCCTTGGCTATTGATCCCAATGAGGAAGTTTTGGTCACTGGAACATCTGATGGCCGAATCCAGGCATGGGGACTAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

980

Amino Acids

108.02

Weight (kDa)

5.58

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 46 - 116 3.5e-17 U-box domain
ARM_LIN_2nd PF23654 156 - 313 2.7e-60 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 322 - 675 4.4e-144 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR5 PF25175 676 - 835 1.5e-07 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 679 - 786 7.9e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 684 - 802 3.1e-09 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 700 - 794 1.3e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 703 - 773 2.5e-07 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 708 - 799 7.1e-09 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 708 - 792 3.4e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 709 - 790 7.6e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 800 - 978 1.2e-07 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 820 - 978 5.5e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 831 - 977 8.2e-07 THOC3 beta-propeller domain
WD40_Prp19 PF24814 834 - 974 5.7e-08 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 880 - 977 8.1e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1557
AasI GACNNNNNNGTC 1 cut(s) 1471
Acc36I ACCTGC 2 cut(s) 263, 2636
AccBSI CCGCTC 2 cut(s) 88, 2433
AccI GTMKAC 3 cut(s) 1336, 1684, 2079
AccIII TCCGGA 1 cut(s) 1499
AciI CCGC 9 cut(s) 22, 57, 86, 117, 268, 386, 1034, 2433, 2734
AcoI YGGCCR 4 cut(s) 1329, 2140, 2367, 2914
AcsI RAATTY 8 cut(s) 125, 274, 513, 1551, 1939, 2205, 2452, 2793
AcuI CTGAAG 3 cut(s) 346, 1890, 2680
AfaI GTAC 4 cut(s) 166, 1270, 1770, 2560
AfiI CCNNNNNNNGG 6 cut(s) 1393, 1796, 2052, 2577, 2923, 2929
AflII CTTAAG 1 cut(s) 1979
AflIII ACRYGT 1 cut(s) 1564
AhlI ACTAGT 1 cut(s) 161
AjnI CCWGG 5 cut(s) 349, 732, 846, 2199, 2922
Alw26I GTCTC 5 cut(s) 188, 699, 1424, 1457, 2715
AlwNI CAGNNNCTG 1 cut(s) 101
Aor13HI TCCGGA 1 cut(s) 1499
ApaI GGGCCC 1 cut(s) 1262
ApoI RAATTY 8 cut(s) 125, 274, 513, 1551, 1939, 2205, 2452, 2793
AseI ATTAAT 1 cut(s) 618
Asp700I GAANNNNTTC 1 cut(s) 2070
AspS9I GGNCC 6 cut(s) 844, 1258, 1259, 1435, 1518, 1966
AsuC2I CCSGG 1 cut(s) 2578
AsuHPI GGTGA 3 cut(s) 415, 1483, 2365
AvaII GGWCC 1 cut(s) 844
AxyI CCTNAGG 2 cut(s) 138, 1920
BaeGI GKGCMC 2 cut(s) 1092, 1262
BalI TGGCCA 2 cut(s) 1331, 2142
BamHI GGATCC 1 cut(s) 2438
BanII GRGCYC 1 cut(s) 1262
BauI CACGAG 1 cut(s) 1673
BbsI GAAGAC 1 cut(s) 1878
BccI CCATC 8 cut(s) 778, 835, 1079, 2021, 2144, 2199, 2654, 2906
BceAI ACGGC 2 cut(s) 1505, 2354
BcgI CGANNNNNNTGC 4 cut(s) 2634, 2668, 2733, 2767
BciT130I CCWGG 5 cut(s) 351, 734, 848, 2201, 2924
BciVI GTATCC 1 cut(s) 1858
BcnI CCSGG 1 cut(s) 2578
BcoDI GTCTC 5 cut(s) 188, 699, 1424, 1457, 2715
BcuI ACTAGT 1 cut(s) 161
BfaI CTAG 9 cut(s) 18, 162, 657, 833, 1515, 2040, 2232, 2279, 2726
BfmI CTRYAG 6 cut(s) 672, 924, 1047, 1093, 1295, 2083
BfrI CTTAAG 1 cut(s) 1979
BfuAI ACCTGC 2 cut(s) 263, 2636
BfuI GTATCC 1 cut(s) 1858
Bme1390I CCNGG 6 cut(s) 351, 734, 848, 2201, 2578, 2924
Bme18I GGWCC 1 cut(s) 844
BmgT120I GGNCC 6 cut(s) 844, 1258, 1259, 1435, 1518, 1966
BmiI GGNNCC 6 cut(s) 26, 1128, 1260, 1437, 2440, 2575
BmrFI CCNGG 6 cut(s) 351, 734, 848, 2201, 2578, 2924
BmrI ACTGGG 2 cut(s) 947, 1648
BmsI GCATC 2 cut(s) 1917, 2498
BmuI ACTGGG 2 cut(s) 947, 1648
BoxI GACNNNNGTC 1 cut(s) 1317
BpiI GAAGAC 1 cut(s) 1878
BplI GAGNNNNNCTC 2 cut(s) 1975, 2007
BpmI CTGGAG 1 cut(s) 1617
Bpu10I CCTNAGC 1 cut(s) 812
BpuEI CTTGAG 4 cut(s) 212, 299, 503, 1293
BpuMI CCSGG 1 cut(s) 2578
BsaBI GATNNNNATC 1 cut(s) 1058
BsaI GGTCTC 2 cut(s) 1457, 2715
BsaJI CCNNGG 6 cut(s) 349, 732, 1698, 1860, 1995, 2865
BsaWI WCCGGW 2 cut(s) 610, 1499
BsaXI ACNNNNNCTCC 4 cut(s) 2655, 2685, 2772, 2802
Bsc4I CCNNNNNNNGG 6 cut(s) 1393, 1796, 2052, 2577, 2923, 2929
Bse118I RCCGGY 1 cut(s) 2570
Bse1I ACTGG 8 cut(s) 182, 202, 942, 1332, 1643, 2323, 2845, 2905
Bse21I CCTNAGG 2 cut(s) 138, 1920
Bse3DI GCAATG 5 cut(s) 817, 1404, 2317, 2413, 2608
Bse8I GATNNNNATC 1 cut(s) 1058
BseAI TCCGGA 1 cut(s) 1499
BseBI CCWGG 5 cut(s) 351, 734, 848, 2201, 2924
BseDI CCNNGG 6 cut(s) 349, 732, 1698, 1860, 1995, 2865
BseGI GGATG 9 cut(s) 1090, 1165, 1258, 1496, 1908, 1932, 2513, 2665, 2789
BseJI GATNNNNATC 1 cut(s) 1058
BseLI CCNNNNNNNGG 6 cut(s) 1393, 1796, 2052, 2577, 2923, 2929
BseMI GCAATG 5 cut(s) 817, 1404, 2317, 2413, 2608
BseMII CTCAG 7 cut(s) 681, 1110, 1435, 1452, 1911, 1961, 2569
BseNI ACTGG 8 cut(s) 182, 202, 942, 1332, 1643, 2323, 2845, 2905
BseRI GAGGAG 1 cut(s) 1821
BseSI GKGCMC 2 cut(s) 1092, 1262
BseYI CCCAGC 1 cut(s) 694
BsgI GTGCAG 1 cut(s) 486
Bsh1285I CGRYCG 1 cut(s) 651
BsiEI CGRYCG 1 cut(s) 651
BsiSI CCGG 4 cut(s) 611, 1500, 2571, 2578
BslFI GGGAC 2 cut(s) 138, 1498
BslI CCNNNNNNNGG 6 cut(s) 1393, 1796, 2052, 2577, 2923, 2929
BsmAI GTCTC 5 cut(s) 188, 699, 1424, 1457, 2715
BsmFI GGGAC 2 cut(s) 138, 1498
BsmI GAATGC 3 cut(s) 822, 2184, 2613
Bso31I GGTCTC 2 cut(s) 1457, 2715
Bsp120I GGGCCC 1 cut(s) 1258
Bsp1286I GDGCHC 2 cut(s) 1092, 1262
Bsp13I TCCGGA 1 cut(s) 1499
BspACI CCGC 9 cut(s) 22, 57, 86, 117, 268, 386, 1034, 2433, 2734
BspCNI CTCAG 7 cut(s) 682, 1111, 1434, 1453, 1912, 1962, 2568
BspEI TCCGGA 1 cut(s) 1499
BspLI GGNNCC 6 cut(s) 26, 1128, 1260, 1437, 2440, 2575
BspMAI CTGCAG 4 cut(s) 928, 1051, 1097, 2087
BspMI ACCTGC 2 cut(s) 263, 2636
BspQI GCTCTTC 1 cut(s) 1709
BspTI CTTAAG 1 cut(s) 1979
BspTNI GGTCTC 2 cut(s) 1457, 2715
BsrBI CCGCTC 2 cut(s) 88, 2433
BsrDI GCAATG 5 cut(s) 817, 1404, 2317, 2413, 2608
BsrFI RCCGGY 1 cut(s) 2570
BsrI ACTGG 8 cut(s) 182, 202, 942, 1332, 1643, 2323, 2845, 2905
BssAI RCCGGY 1 cut(s) 2570
BssECI CCNNGG 6 cut(s) 349, 732, 1698, 1860, 1995, 2865
BssSI CACGAG 1 cut(s) 1673
BssT1I CCWWGG 4 cut(s) 1698, 1860, 1995, 2865
Bst2BI CACGAG 1 cut(s) 1673
Bst2UI CCWGG 5 cut(s) 351, 734, 848, 2201, 2924
Bst4CI ACNGT 9 cut(s) 676, 979, 1170, 1388, 1612, 1836, 2270, 2627, 2852
Bst6I CTCTTC 3 cut(s) 1032, 1386, 1709
BstAFI CTTAAG 1 cut(s) 1979
BstC8I GCNNGC 6 cut(s) 386, 1540, 1669, 1744, 1893, 2736
BstF5I GGATG 9 cut(s) 1090, 1165, 1258, 1496, 1908, 1932, 2513, 2665, 2789
BstMAI GTCTC 5 cut(s) 188, 699, 1424, 1457, 2715
BstMCI CGRYCG 1 cut(s) 651
BstMWI GCNNNNNNNGC 9 cut(s) 394, 559, 1087, 1115, 1124, 1442, 1451, 1892, 2517
BstNI CCWGG 5 cut(s) 351, 734, 848, 2201, 2924
BstNSI RCATGY 2 cut(s) 1746, 1777
BstPAI GACNNNNGTC 1 cut(s) 1317
BstSCI CCNGG 6 cut(s) 349, 732, 846, 2199, 2576, 2922
BstSFI CTRYAG 6 cut(s) 672, 924, 1047, 1093, 1295, 2083
BstSLI GKGCMC 2 cut(s) 1092, 1262
BstV2I GAAGAC 1 cut(s) 1878
BstX2I RGATCY 2 cut(s) 1059, 2438
BstXI CCANNNNNNTGG 2 cut(s) 1679, 2150
BstYI RGATCY 2 cut(s) 1059, 2438
Bsu36I CCTNAGG 2 cut(s) 138, 1920
BsuI GTATCC 1 cut(s) 1858
BtsCI GGATG 9 cut(s) 1090, 1165, 1258, 1496, 1908, 1932, 2513, 2665, 2789
BtsI GCAGTG 7 cut(s) 933, 964, 1090, 1475, 2092, 2527, 2676
BveI ACCTGC 2 cut(s) 263, 2636
Cac8I GCNNGC 6 cut(s) 386, 1540, 1669, 1744, 1893, 2736
CaiI CAGNNNCTG 1 cut(s) 101
Cfr10I RCCGGY 1 cut(s) 2570
Cfr13I GGNCC 6 cut(s) 844, 1258, 1259, 1435, 1518, 1966
CseI GACGC 1 cut(s) 308
CsiI ACCWGGT 1 cut(s) 846
Csp6I GTAC 4 cut(s) 165, 1269, 1769, 2559
CviQI GTAC 4 cut(s) 165, 1269, 1769, 2559
DrdI GACNNNNNNGTC 1 cut(s) 1471
DseDI GACNNNNNNGTC 1 cut(s) 1471
EaeI YGGCCR 4 cut(s) 1329, 2140, 2367, 2914
Eam1104I CTCTTC 3 cut(s) 1032, 1386, 1709
EarI CTCTTC 3 cut(s) 1032, 1386, 1709
EciI GGCGGA 1 cut(s) 37
Eco130I CCWWGG 4 cut(s) 1698, 1860, 1995, 2865
Eco147I AGGCCT 1 cut(s) 1697
Eco24I GRGCYC 1 cut(s) 1262
Eco31I GGTCTC 2 cut(s) 1457, 2715
Eco32I GATATC 1 cut(s) 1957
Eco47I GGWCC 1 cut(s) 844
Eco57I CTGAAG 3 cut(s) 346, 1890, 2680
Eco81I CCTNAGG 2 cut(s) 138, 1920
EcoO109I RGGNCCY 4 cut(s) 1258, 1259, 1435, 1966
EcoRII CCWGG 5 cut(s) 349, 732, 846, 2199, 2922
EcoRV GATATC 1 cut(s) 1957
EcoT14I CCWWGG 4 cut(s) 1698, 1860, 1995, 2865
EcoT38I GRGCYC 1 cut(s) 1262
ErhI CCWWGG 4 cut(s) 1698, 1860, 1995, 2865
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FaqI GGGAC 2 cut(s) 138, 1498
FauI CCCGC 3 cut(s) 275, 393, 2727
FauNDI CATATG 1 cut(s) 208
FblI GTMKAC 3 cut(s) 1336, 1684, 2079
FokI GGATG 9 cut(s) 1097, 1172, 1265, 1483, 1895, 1939, 2520, 2672, 2796
FriOI GRGCYC 1 cut(s) 1262
FspBI CTAG 9 cut(s) 18, 162, 657, 833, 1515, 2040, 2232, 2279, 2726
GsaI CCCAGC 1 cut(s) 698
GsuI CTGGAG 1 cut(s) 1617
HapII CCGG 4 cut(s) 611, 1500, 2571, 2578
HgaI GACGC 1 cut(s) 308
HincII GTYRAC 2 cut(s) 739, 2718
HindII GTYRAC 2 cut(s) 739, 2718
HpaII CCGG 4 cut(s) 611, 1500, 2571, 2578
HphI GGTGA 3 cut(s) 415, 1483, 2365
Hpy166II GTNNAC 9 cut(s) 739, 853, 1337, 1491, 1685, 1771, 2080, 2169, 2718
Hpy188III TCNNGA 9 cut(s) 702, 799, 1310, 1500, 1596, 1790, 1919, 2210, 2528
Hpy8I GTNNAC 9 cut(s) 739, 853, 1337, 1491, 1685, 1771, 2080, 2169, 2718
HpyAV CCTTC 8 cut(s) 370, 429, 1036, 1535, 1700, 1796, 2309, 2341
HpyCH4III ACNGT 9 cut(s) 676, 979, 1170, 1388, 1612, 1836, 2270, 2627, 2852
HpyCH4IV ACGT 3 cut(s) 1487, 1534, 1564
HpyF10VI GCNNNNNNNGC 9 cut(s) 394, 559, 1087, 1115, 1124, 1442, 1451, 1892, 2517
HpySE526I ACGT 3 cut(s) 1487, 1534, 1564
Kpn2I TCCGGA 1 cut(s) 1499
LguI GCTCTTC 1 cut(s) 1709
LmnI GCTCC 5 cut(s) 1132, 1808, 2430, 2579, 2663
LweI GCATC 2 cut(s) 1917, 2498
MabI ACCWGGT 1 cut(s) 846
MaeI CTAG 9 cut(s) 18, 162, 657, 833, 1515, 2040, 2232, 2279, 2726
MaeII ACGT 3 cut(s) 1487, 1534, 1564
MaeIII GTNAC 7 cut(s) 184, 979, 1473, 2227, 2371, 2542, 2896
MbiI CCGCTC 2 cut(s) 88, 2433
MflI RGATCY 2 cut(s) 1059, 2438
MhlI GDGCHC 2 cut(s) 1092, 1262
MlsI TGGCCA 2 cut(s) 1331, 2142
MluNI TGGCCA 2 cut(s) 1331, 2142
MlyI GAGTC 4 cut(s) 533, 734, 848, 2030
MmeI TCCRAC 1 cut(s) 2637
Mox20I TGGCCA 2 cut(s) 1331, 2142
MroI TCCGGA 1 cut(s) 1499
MroXI GAANNNNTTC 1 cut(s) 2070
MscI TGGCCA 2 cut(s) 1331, 2142
MslI CAYNNNNRTG 1 cut(s) 2148
Msp20I TGGCCA 2 cut(s) 1331, 2142
MspA1I CMGCKG 3 cut(s) 923, 1034, 1631
MspCI CTTAAG 1 cut(s) 1979
MspI CCGG 4 cut(s) 611, 1500, 2571, 2578
MspR9I CCNGG 6 cut(s) 351, 734, 848, 2201, 2578, 2924
Mva1269I GAATGC 3 cut(s) 822, 2184, 2613
MvaI CCWGG 5 cut(s) 351, 734, 848, 2201, 2924
MwoI GCNNNNNNNGC 9 cut(s) 394, 559, 1087, 1115, 1124, 1442, 1451, 1892, 2517
NciI CCSGG 1 cut(s) 2578
NdeI CATATG 1 cut(s) 208
NlaIV GGNNCC 6 cut(s) 26, 1128, 1260, 1437, 2440, 2575
NmeAIII GCCGAG 2 cut(s) 1392, 1795
NmuCI GTSAC 6 cut(s) 184, 979, 1473, 2371, 2542, 2896
NspI RCATGY 2 cut(s) 1746, 1777
PaeI GCATGC 1 cut(s) 1746
PceI AGGCCT 1 cut(s) 1697
PciSI GCTCTTC 1 cut(s) 1709
PcsI WCGNNNNNNNCGW 2 cut(s) 550, 975
PctI GAATGC 3 cut(s) 822, 2184, 2613
PdmI GAANNNNTTC 1 cut(s) 2070
PfoI TCCNGGA 2 cut(s) 2199, 2576
PleI GAGTC 4 cut(s) 532, 734, 848, 2030
PpsI GAGTC 4 cut(s) 532, 734, 848, 2030
PshAI GACNNNNGTC 1 cut(s) 1317
PshBI ATTAAT 1 cut(s) 618
PsiI TTATAA 1 cut(s) 1557
Psp6I CCWGG 5 cut(s) 349, 732, 846, 2199, 2922
PspFI CCCAGC 1 cut(s) 694
PspGI CCWGG 5 cut(s) 349, 732, 846, 2199, 2922
PspN4I GGNNCC 6 cut(s) 26, 1128, 1260, 1437, 2440, 2575
PspOMI GGGCCC 1 cut(s) 1258
PspPI GGNCC 6 cut(s) 844, 1258, 1259, 1435, 1518, 1966
PsrI GAACNNNNNNTAC 2 cut(s) 2542, 2574
PstI CTGCAG 4 cut(s) 928, 1051, 1097, 2087
PstNI CAGNNNCTG 1 cut(s) 101
PsuI RGATCY 2 cut(s) 1059, 2438
PvuII CAGCTG 2 cut(s) 923, 1631
RsaI GTAC 4 cut(s) 166, 1270, 1770, 2560
RsaNI GTAC 4 cut(s) 165, 1269, 1769, 2559
RseI CAYNNNNRTG 1 cut(s) 2148
SapI GCTCTTC 1 cut(s) 1709
Sau96I GGNCC 6 cut(s) 844, 1258, 1259, 1435, 1518, 1966
SchI GAGTC 4 cut(s) 533, 734, 848, 2030
ScrFI CCNGG 6 cut(s) 351, 734, 848, 2201, 2578, 2924
SduI GDGCHC 2 cut(s) 1092, 1262
SexAI ACCWGGT 1 cut(s) 846
SfaNI GCATC 2 cut(s) 1917, 2498
SfcI CTRYAG 6 cut(s) 672, 924, 1047, 1093, 1295, 2083
SinI GGWCC 1 cut(s) 844
SmiMI CAYNNNNRTG 1 cut(s) 2148
SmlI CTYRAG 5 cut(s) 191, 314, 518, 1308, 1979
SmoI CTYRAG 5 cut(s) 191, 314, 518, 1308, 1979
SpeI ACTAGT 1 cut(s) 161
SphI GCATGC 1 cut(s) 1746
SseBI AGGCCT 1 cut(s) 1697
SsiI CCGC 9 cut(s) 22, 57, 86, 117, 268, 386, 1034, 2433, 2734
SspI AATATT 3 cut(s) 1429, 1848, 2133
SspMI CTAG 9 cut(s) 18, 162, 657, 833, 1515, 2040, 2232, 2279, 2726
StuI AGGCCT 1 cut(s) 1697
StyD4I CCNGG 6 cut(s) 349, 732, 846, 2199, 2576, 2922
StyI CCWWGG 4 cut(s) 1698, 1860, 1995, 2865
TaaI ACNGT 9 cut(s) 676, 979, 1170, 1388, 1612, 1836, 2270, 2627, 2852
TaiI ACGT 3 cut(s) 1490, 1537, 1567
TaqI TCGA 1 cut(s) 651
TaqII GACCGA 1 cut(s) 637
TatI WGTACW 1 cut(s) 1268
TseFI GTSAC 6 cut(s) 184, 979, 1473, 2371, 2542, 2896
Tsp45I GTSAC 6 cut(s) 184, 979, 1473, 2371, 2542, 2896
TspGWI ACGGA 2 cut(s) 1901, 2109
Vha464I CTTAAG 1 cut(s) 1979
VpaK11BI GGWCC 1 cut(s) 844
VspI ATTAAT 1 cut(s) 618
XapI RAATTY 8 cut(s) 125, 274, 513, 1551, 1939, 2205, 2452, 2793
XceI RCATGY 2 cut(s) 1746, 1777
XcmI CCANNNNNNNNNTGG 1 cut(s) 116
XmiI GTMKAC 3 cut(s) 1336, 1684, 2079
XmnI GAANNNNTTC 1 cut(s) 2070
XspI CTAG 9 cut(s) 18, 162, 657, 833, 1515, 2040, 2232, 2279, 2726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.