MD15G1011400.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001838
Physical Location & Seq
Forward (+)
629335 .. 636133
6799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1011400.v1.1.491

Sequence Viewer

Length: 4452 bp
ATGGCGAGAAACTATGGATTTGCAATGGGCCAACAGGACATTGTTAGGATCTTAACGACCACAGTTGATGGATTTATCAGGGATCAGCTGATCAACAAAGACCAAAGAGCCCAACACAGGGAACAATGTGCTGAGAGATTGGCAGCACAAGATGGAAGTTGTGACAGAGATACTGAGGTTCGATACTCTGATCAAGCAGTGCTGGCAAATCTGGACTGGGGTATTGAATCCCTCGAAGAAGCTATCGGAACTTCAAACATGGAAACTAAGCTTGCAAGACTTGATCATGCTGAAAAAATGTTGCAAGTTTGTGCAATGTTGAATTGTGACCAGAGAACTGCTGGAGTCCCCAATTATTATCTATCTGCTTGGGCACACCTCAACCTAGCATACCTTTCGAAATTGCGCAACAATATCCAACTAGCGGTTCTCCATGTGATTGAGATGTTTATAGTTGACCCTTTCTTCTCAAGGATAGATTTTGCTCCCGAGCTATGGAAAAATCTGTTTCTTCCACACATGAGCTCGATTATAGGGTGGTATTCGGAGCAGAGGCACAGACTTGTGATGGAAGTAATCCCTGATTCTGCTGATCTGTCTTTTACAGCTGATCTTGATCATCTTTTTAACGAGTCAGTGGTCTGTTCGATGAGGCCTGATCAGGGTGAGAAGTTGCAAAAGCTGGAGCAGCTTTATGGAGAATCGTTGGATGAGAACACGCGGCTTTACGCAAAGTACTTTAAGGATTGCATGAACACTGATTCAACAACCCCAAGTAAAAAGGTGGTTCCAATGCTGCCAATCGCAGAGGCACCAATGACTCCGTTGCATGAGGTCAGCCACTCAATTCCTGATTTCGTAAAGTTTGGTCCTATCTTGCCCAAGAGTGCCGGTTTTTCTCCCGTCCTCAAGTCTAAAGATAGCACAAGAGAAACAAGCAGAATGAATTTAACTTCTGCATCCTTACTGAAATTGGAGTCTGCTAGATGGGATCCTCAGGAAGGCATCCCTGAGGAAAATGAAGACGAATCAGATTACGAGGCTAACGATGCTATTGTGGCTTTGGATGAAAAGGAAAGTGTGGAAAATGTACAGCCATCTGTAAGAAAGAGTCGAATACACACTCCTTCAATCTTTTCTCCTTTTCAATCGCCTAGAACTTCTCCAAAGATTTTATCTCCAAAGCCAGACAGTGTGCAGTCTAAAAACGAAGCCACTTCTGTATTGCGCTTATTTTCCACTCGCATGACGGATTCAGCTATTACAACCTCTTTGCCTGCATCTCCAGTCATTAGTAATGAGTTCAGCATCAGCTCAGCAGACTCCGATTGCGAAGTGATAACTCCGAAAAGTTGCAGGAAAACTTCCAGCCGGACAGAAAGTGATATTTCGGATCATGTGAATGGCCAAAAGTCGAAAGATAGTCCACGTAACGAAAATGATGAAGGAAGCCACAGTTGCAGTTCAATCCCATATTCCGATAGACTGACACATAAATCAAGGCCGCCTAAGGATTTTGTTTGCCCTATCACTGGCCAGATTTTCGGTGATCCAGTTACCCTCGAAACAGGCCAGACATACGAAAGAAAAGCAATCCAAGAATGGCTCAAACGAGGAAACACGACATGCCCCATTACAAGGCAACCCATTGCGACTTCTACACTGCCAAAAACGAACTACGTTCTGAAAAGACTAATAACATCATGGAAAGAAGAGCATCCTGAACTTGCTCAAGAGTCTCCGTACTCTGAATCACCAAAAATATCATTCGACCCCTCCTCCGGGAAAGAAACTCATTCAGCAACAGCCACTTCACAGAGAACACCTAGTTTCCTAGGTCACAGCAATGCCGATGATTATACCAATATCATCCAACGGAACAAAAGATTCATGCGAGTAACGGTTGCTACATCACCAACAAGTGTGATATCTCAAGCTGCAGTCGAGACAATTATCAGCAGTTTGAAACCTCATGTTTCATGTCTATGCACTTCAGAAAACTTACAAGAATGTGAGGCAGCCGTGTTGTCAATAGCCAGACTATGGAAGGACTCAAAGGCTGATCCCGCAGTGCATTCTTACTTATCTGAACTGACAACTGTCAACGGATTCATCGAAATACTTTCAGCTTCCCTGAGCCGAGAGGTCCTCAGATCATCAATTTACATCCTCTCAGAGCTAATATTTTCAGAAGAAAGTGTTGGAGAAACTCTTACGAGTGTAGATTCTGACCTCGATTGCCTCGCTACTCTGCTAAAGAATGGACTAGCTGAGGCTGCTGTTCTTATTTACCAGTTGAGGCCGGCATTTGCTCAGCTTTCAGTACATGATCTCATACCCTCCCTTGTCCAGATAATTCTAAGCAAAACTGAGGAATTGGATGATCTTCAGTTGGTGATGGAGCCTAAAGATGCTGCATTGGCAATAATTGAGCAAATCTTGATGGGAGGAGACGAAAACAGCAGATCTATTAACGCGTTGAGTGTTATATCTGCAAACGGGATTCCTGGACTTGTAAGGTTTTTGGATAAAGCAGAAGGGACAAGGTCGATTGTTTCAATTTTATTATGTTGTATGCAAGCTGAAAAAAGTTGCAGGAACTTGATAGCAAATAGAATCGATCTGTCTCCTGTTCTTGAATTGTTTCATGCCGGAAATGATTGCGTGCGAGGCATATGTGTAGAATTTCTTTCGGAGCTGGTTCAGTTGAACAGGAGAACATTGAGCAATCAGATCCTGCACATGATTAAAGACGAAGGAGCATTCAGTACGATGCACACTTTTCTCGTGTATCTCCAAATGGCGCCGATGGAGCAGCAACCTACAATCGCTTCTCTTCTTCTTCAGCTTGATCTTCTGGTTGAGCCGTGGAAGATGAGCATTTACAGGGAAGATGCAATTGAGGCATTAATTGAAGCGATGCGGCGGAAAGACTTCCCTAATTCTCAAATGATGGCTCTGGATACCTTGTTATCTCTTACCGGACGAGTGACTTCCTCAGGGGATTCGAATACTGAAGCCTGGTTACTCAAGACTGCAGGGTTTGATCAACCGTACAATGCTTTGATGAAGGCAGAGCGGCTGAGGAAACACGACAATGATTTCATGGAAACAATGGACGAAGAAGAGAAAGCTGTAACCTCTTGGCAGAAAAGGGTCGCTTTCGTACTTTGCAACCACGAAAAGGGGTCGATTTTTAAAGCTTTGGAAGAATGTCTCAAGAGTAATTCATTAGAGATGGCAAAGTCATGTCTTGTCATTGCTACATGGCTAACACACATGCTCTCTGTACTTCCTGATACTGGCGTGAAAAGTGGCGCTTGCAAGGCCTTGCTTGACGAATTCATGAATGTTCTCCAATCGTCGAATAATATGGAGGAGAAGATTTTGGCAACTCTTGCCTTGAAATCTTTTGTCAACGATCCAGCTGCACTTGAAGCACTAAGAGTGTATGCTAAACCCATCTACAAAACCTTGAGAAAGCTCAAGAAGTATGCATTCGTGGCAAACGACATAATGAAAGCGTTGATGAACTTGTCATCTATTGATATAGCAGAGCTGTGGAGTTGTTCCGAAGTGCTTGAATTGGAATCATCCACGAACGGGGAGGTTCTGTCTTTGCTTCATCTGAAAAGTCGCATTTTAAGCAGCCATTCGGATGGGACTATAAAGGTATGGGAGGCAGGGAAGAAGGTGCCACGATTGATTCAAGAAGTTCGTGAGCACGCGAAGGCTGTTACATGCCTCTATATTTCACCTACAGGCGACAAATTATACAGCGGTTCTTTAGACAAAACAATTCGAATATGGGCGATTAAAGCCGAAGAGATTCATTGTCTCCAAGTTCATGACGTGAAGGATGCAGTTTATGAGTTGGTAGCGAATGGTAAAATGGCGTGCTTCGTTTCTCAGGGCACCGGAGTTAAGGTGTACGAGTGGTCTGGAGTTCAAAAGCACATAAACTTCAACAAATACGTAAAGAGCCTTGCCATTGCCGGGACTAGTTTGTACTGTGGTTGCTCTGGCTACAGCATCCAGGAGGTTGATTTGGGCAAATACACATCAAGCACATTCTACTCTGGCACAAGAAAGCTGCTTGGGAAACAGGTCATCTACTCCCTCCATATCCACGACGGCATTCTATATGCTGGCGGTTCATCAGTAGATGCCTCAGCCGGAAAGATGTTCTCGCTTCCCAACAAGGCAGTGGTAGGATCGTTCACAACCGGGCTTGACATCCAACGAATAACCATCAACAACGACTTGATATTCACGGCCAGCAAGTGCGGGACGATTGAGGTTTGGTTGAAGGAAAGGTTCACACGAATTGCTTCGTTCAGAATGGTTAACGGAGGACACGCCAAGATTACATCCTTGGCAGCAGACATGGATGGAGGAATGCTGTATGCGGGTTCATCAGATGGCAGAATTCAGGTTTGGGCACTAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1484

Amino Acids

164.79

Weight (kDa)

5.66

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 11 - 265 3.9e-74 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 503 - 573 2.3e-15 U-box domain
ARM_LIN_2nd PF23654 650 - 811 4.1e-60 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 812 - 1174 7.4e-118 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
WD40_Prp19 PF24814 1203 - 1305 2.6e-09 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 1205 - 1312 3.4e-11 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 1210 - 1314 3.3e-11 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1213 - 1287 9e-10 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 1213 - 1275 3.9e-07 WDR3 second beta-propeller domain
WD40 PF00400 1235 - 1270 2.2e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 407
AccB1I GGYRCC 4 cut(s) 811, 2803, 3694, 3914
AccB7I CCANNNNNTGG 1 cut(s) 2043
AccBSI CCGCTC 1 cut(s) 3079
AccII CGCG 3 cut(s) 721, 2477, 3728
AcoI YGGCCR 3 cut(s) 1405, 1534, 4275
AcsI RAATTY 4 cut(s) 946, 2684, 3341, 4428
AcuI CTGAAG 4 cut(s) 1977, 2372, 2828, 3036
AcyI GRCGYC 1 cut(s) 2804
AflIII ACRYGT 1 cut(s) 2475
AhlI ACTAGT 1 cut(s) 4001
AjiI CACGTC 1 cut(s) 3853
AjnI CCWGG 3 cut(s) 2506, 3020, 4035
AjuI GAANNNNNNNTTGG 6 cut(s) 411, 443, 2184, 2216, 4420, 4452
Alw21I GWGCWC 2 cut(s) 527, 3726
Alw26I GTCTC 6 cut(s) 1743, 1940, 2445, 2631, 3221, 3842
AlwNI CAGNNNCTG 1 cut(s) 2737
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 9 cut(s) 28, 653, 1405, 1502, 1534, 1570, 2300, 3327, 4275
ApoI RAATTY 4 cut(s) 946, 2684, 3341, 4428
AseI ATTAAT 1 cut(s) 2909
Asp700I GAANNNNTTC 4 cut(s) 2643, 2933, 3828, 4330
AspA2I CCTAGG 1 cut(s) 1834
AspLEI GCGC 4 cut(s) 408, 1230, 2806, 3320
AspS9I GGNCC 3 cut(s) 28, 869, 2146
AsuC2I CCSGG 3 cut(s) 1783, 3997, 4228
AsuHPI GGTGA 6 cut(s) 677, 1559, 1746, 1905, 2407, 3747
AsuII TTCGAA 3 cut(s) 398, 3008, 3802
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 2 cut(s) 869, 2146
AvrII CCTAGG 1 cut(s) 1834
AxyI CCTNAGG 4 cut(s) 996, 1011, 1509, 2998
BaeGI GKGCMC 3 cut(s) 376, 3917, 4444
BaeI ACNNNNGTAYC 4 cut(s) 162, 195, 3291, 3324
BalI TGGCCA 2 cut(s) 1407, 1536
BamHI GGATCC 1 cut(s) 991
BanI GGYRCC 4 cut(s) 811, 2803, 3694, 3914
BanII GRGCYC 2 cut(s) 112, 527
BarI GAAGNNNNNNTAC 2 cut(s) 3009, 3041
BauI CACGAG 1 cut(s) 2786
BbsI GAAGAC 1 cut(s) 1029
Bbv12I GWGCWC 2 cut(s) 527, 3726
BbvCI CCTCAGC 3 cut(s) 2271, 3083, 4171
BceAI ACGGC 4 cut(s) 2006, 2851, 4150, 4290
BcgI CGANNNNNNTGC 4 cut(s) 378, 412, 3410, 3444
BciT130I CCWGG 3 cut(s) 2508, 3022, 4037
BciVI GTATCC 1 cut(s) 2956
BclI TGATCA 6 cut(s) 90, 190, 283, 616, 658, 3046
BcnI CCSGG 3 cut(s) 1783, 3997, 4228
BcoDI GTCTC 6 cut(s) 1743, 1940, 2445, 2631, 3221, 3842
BcuI ACTAGT 1 cut(s) 4001
BfmI CTRYAG 4 cut(s) 1938, 3036, 3759, 4027
BfoI RGCGCY 2 cut(s) 2807, 3321
BfuI GTATCC 1 cut(s) 2956
BglII AGATCT 1 cut(s) 2465
BlnI CCTAGG 1 cut(s) 1834
BlpI GCTNAGC 2 cut(s) 1315, 2313
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 6 cut(s) 1783, 2508, 3022, 3997, 4037, 4228
Bme18I GGWCC 2 cut(s) 869, 2146
BmeT110I CYCGRG 1 cut(s) 488
BmgBI CACGTC 1 cut(s) 3853
BmgT120I GGNCC 3 cut(s) 28, 869, 2146
BmiI GGNNCC 7 cut(s) 789, 813, 993, 2403, 2805, 3696, 3916
BmrFI CCNGG 6 cut(s) 1783, 2508, 3022, 3997, 4037, 4228
BmrI ACTGGG 1 cut(s) 226
BmuI ACTGGG 1 cut(s) 226
BoxI GACNNNNGTC 1 cut(s) 2099
BpiI GAAGAC 1 cut(s) 1029
BplI GAGNNNNNCTC 2 cut(s) 2133, 2165
BpmI CTGGAG 4 cut(s) 363, 704, 1269, 3963
Bpu10I CCTNAGC 4 cut(s) 2135, 2271, 3083, 4171
Bpu1102I GCTNAGC 2 cut(s) 1315, 2313
Bpu14I TTCGAA 3 cut(s) 398, 3008, 3802
BpuEI CTTGAG 8 cut(s) 454, 893, 1716, 1917, 3014, 3203, 3470, 3496
BpuMI CCSGG 3 cut(s) 1783, 3997, 4228
Bsa29I ATCGAT 1 cut(s) 2619
BsaAI YACGTR 2 cut(s) 1430, 3976
BsaBI GATNNNNATC 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 2804
BsaJI CCNNGG 3 cut(s) 1834, 2867, 4374
BsaWI WCCGGW 2 cut(s) 2981, 3917
BsaXI ACNNNNNCTCC 6 cut(s) 1763, 1793, 3599, 3629, 3912, 3942
Bse118I RCCGGY 2 cut(s) 890, 2302
Bse1I ACTGG 6 cut(s) 221, 1286, 1537, 1553, 2293, 3307
Bse21I CCTNAGG 4 cut(s) 996, 1011, 1509, 2998
Bse3DI GCAATG 6 cut(s) 30, 321, 1647, 1852, 3258, 3990
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 3 cut(s) 2508, 3022, 4037
BseCI ATCGAT 1 cut(s) 2619
BseDI CCNNGG 3 cut(s) 1834, 2867, 4374
BseJI GATNNNNATC 1 cut(s) 615
BseMI GCAATG 6 cut(s) 30, 321, 1647, 1852, 3258, 3990
BseNI ACTGG 6 cut(s) 221, 1286, 1537, 1553, 2293, 3307
BseRI GAGGAG 3 cut(s) 1768, 2463, 3392
BseSI GKGCMC 3 cut(s) 376, 3917, 4444
BsgI GTGCAG 3 cut(s) 1217, 2723, 3414
Bsh1236I CGCG 3 cut(s) 721, 2477, 3728
BshFI GGCC 9 cut(s) 30, 655, 1407, 1504, 1536, 1572, 2302, 3329, 4277
BshNI GGYRCC 4 cut(s) 811, 2803, 3694, 3914
BshVI ATCGAT 1 cut(s) 2619
BsiHKAI GWGCWC 2 cut(s) 527, 3726
BsiHKCI CYCGRG 1 cut(s) 488
BslFI GGGAC 5 cut(s) 332, 2554, 3676, 4012, 4303
BsmAI GTCTC 6 cut(s) 1743, 1940, 2445, 2631, 3221, 3842
BsmBI CGTCTC 1 cut(s) 2445
BsmFI GGGAC 5 cut(s) 332, 2554, 3676, 4012, 4303
BsmI GAATGC 5 cut(s) 2074, 2762, 3497, 4137, 4404
BsnI GGCC 9 cut(s) 30, 655, 1407, 1504, 1536, 1572, 2302, 3329, 4277
BsoBI CYCGRG 1 cut(s) 488
Bsp119I TTCGAA 3 cut(s) 398, 3008, 3802
Bsp1286I GDGCHC 6 cut(s) 112, 376, 527, 3726, 3917, 4444
Bsp1407I TGTACA 1 cut(s) 1090
Bsp1720I GCTNAGC 2 cut(s) 1315, 2313
BspANI GGCC 9 cut(s) 30, 655, 1407, 1504, 1536, 1572, 2302, 3329, 4277
BspDI ATCGAT 1 cut(s) 2619
BspFNI CGCG 3 cut(s) 721, 2477, 3728
BspHI TCATGA 2 cut(s) 3345, 3847
BspLI GGNNCC 7 cut(s) 789, 813, 993, 2403, 2805, 3696, 3916
BspMAI CTGCAG 2 cut(s) 1942, 3040
BspQI GCTCTTC 1 cut(s) 1707
BspT104I TTCGAA 3 cut(s) 398, 3008, 3802
BspT107I GGYRCC 4 cut(s) 811, 2803, 3694, 3914
BsrBI CCGCTC 1 cut(s) 3079
BsrDI GCAATG 6 cut(s) 30, 321, 1647, 1852, 3258, 3990
BsrFI RCCGGY 2 cut(s) 890, 2302
BsrGI TGTACA 1 cut(s) 1090
BsrI ACTGG 6 cut(s) 221, 1286, 1537, 1553, 2293, 3307
BssAI RCCGGY 2 cut(s) 890, 2302
BssECI CCNNGG 3 cut(s) 1834, 2867, 4374
BssNI GRCGYC 1 cut(s) 2804
BssSI CACGAG 1 cut(s) 2786
BssT1I CCWWGG 2 cut(s) 1834, 4374
Bst2BI CACGAG 1 cut(s) 2786
Bst2UI CCWGG 3 cut(s) 2508, 3022, 4037
Bst4CI ACNGT 7 cut(s) 64, 1193, 1457, 1903, 2101, 3054, 4013
Bst6I CTCTTC 4 cut(s) 1707, 2841, 3120, 3819
BstACI GRCGYC 1 cut(s) 2804
BstAPI GCANNNNNTGC 1 cut(s) 3398
BstAUI TGTACA 1 cut(s) 1090
BstBAI YACGTR 2 cut(s) 1430, 3976
BstBI TTCGAA 3 cut(s) 398, 3008, 3802
BstDSI CCRYGG 1 cut(s) 2867
BstENI CCTNNNNNAGG 1 cut(s) 999
BstFNI CGCG 3 cut(s) 721, 2477, 3728
BstH2I RGCGCY 2 cut(s) 2807, 3321
BstHHI GCGC 4 cut(s) 408, 1230, 2806, 3320
BstMAI GTCTC 6 cut(s) 1743, 1940, 2445, 2631, 3221, 3842
BstNI CCWGG 3 cut(s) 2508, 3022, 4037
BstNSI RCATGY 3 cut(s) 1629, 3283, 3744
BstPAI GACNNNNGTC 1 cut(s) 2099
BstSCI CCNGG 6 cut(s) 1781, 2506, 3020, 3995, 4035, 4226
BstSFI CTRYAG 4 cut(s) 1938, 3036, 3759, 4027
BstSLI GKGCMC 3 cut(s) 376, 3917, 4444
BstSNI TACGTA 1 cut(s) 3976
BstUI CGCG 3 cut(s) 721, 2477, 3728
BstV2I GAAGAC 1 cut(s) 1029
BstX2I RGATCY 4 cut(s) 48, 991, 2465, 2733
BstXI CCANNNNNNTGG 1 cut(s) 3659
BstYI RGATCY 4 cut(s) 48, 991, 2465, 2733
Bsu15I ATCGAT 1 cut(s) 2619
Bsu36I CCTNAGG 4 cut(s) 996, 1011, 1509, 2998
BsuI GTATCC 1 cut(s) 2956
BsuRI GGCC 9 cut(s) 30, 655, 1407, 1504, 1536, 1572, 2302, 3329, 4277
BsuTUI ATCGAT 1 cut(s) 2619
BtgI CCRYGG 1 cut(s) 2867
BtgZI GCGATG 1 cut(s) 2933
BtrI CACGTC 1 cut(s) 3853
BtsI GCAGTG 4 cut(s) 204, 1661, 2076, 4212
BtsIMutI CAGTG 8 cut(s) 204, 642, 756, 1198, 1530, 1661, 2076, 4212
CaiI CAGNNNCTG 1 cut(s) 2737
CciI TCATGA 2 cut(s) 3345, 3847
CfoI GCGC 4 cut(s) 408, 1230, 2806, 3320
Cfr10I RCCGGY 2 cut(s) 890, 2302
Cfr13I GGNCC 3 cut(s) 28, 869, 2146
ClaI ATCGAT 1 cut(s) 2619
DinI GGCGCC 1 cut(s) 2805
DraI TTTAAA 1 cut(s) 3199
EaeI YGGCCR 3 cut(s) 1405, 1534, 4275
Eam1104I CTCTTC 4 cut(s) 1707, 2841, 3120, 3819
EarI CTCTTC 4 cut(s) 1707, 2841, 3120, 3819
EciI GGCGGA 1 cut(s) 2941
Ecl136II GAGCTC 1 cut(s) 525
Eco105I TACGTA 1 cut(s) 3976
Eco130I CCWWGG 2 cut(s) 1834, 4374
Eco147I AGGCCT 2 cut(s) 655, 3329
Eco24I GRGCYC 2 cut(s) 112, 527
Eco32I GATATC 1 cut(s) 1929
Eco47I GGWCC 2 cut(s) 869, 2146
Eco53kI GAGCTC 1 cut(s) 525
Eco57I CTGAAG 4 cut(s) 1977, 2372, 2828, 3036
Eco81I CCTNAGG 4 cut(s) 996, 1011, 1509, 2998
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 525
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoO109I RGGNCCY 1 cut(s) 2146
EcoRI GAATTC 2 cut(s) 3341, 4428
EcoRII CCWGG 3 cut(s) 2506, 3020, 4035
EcoRV GATATC 1 cut(s) 1929
EcoT14I CCWWGG 2 cut(s) 1834, 4374
EcoT22I ATGCAT 1 cut(s) 3499
EcoT38I GRGCYC 2 cut(s) 112, 527
EgeI GGCGCC 1 cut(s) 2805
EheI GGCGCC 1 cut(s) 2805
ErhI CCWWGG 2 cut(s) 1834, 4374
Esp3I CGTCTC 1 cut(s) 2445
FalI AAGNNNNNCTT 4 cut(s) 3145, 3177, 3304, 3336
FaqI GGGAC 5 cut(s) 332, 2554, 3676, 4012, 4303
FauI CCCGC 3 cut(s) 2074, 4280, 4402
FauNDI CATATG 1 cut(s) 2675
FbaI TGATCA 6 cut(s) 90, 190, 283, 616, 658, 3046
FriOI GRGCYC 2 cut(s) 112, 527
FspI TGCGCA 1 cut(s) 407
GlaI GCGC 4 cut(s) 407, 1229, 2805, 3319
GsuI CTGGAG 4 cut(s) 363, 704, 1269, 3963
HaeII RGCGCY 2 cut(s) 2807, 3321
HaeIII GGCC 9 cut(s) 30, 655, 1407, 1504, 1536, 1572, 2302, 3329, 4277
HhaI GCGC 4 cut(s) 408, 1230, 2806, 3320
Hin1I GRCGYC 1 cut(s) 2804
Hin6I GCGC 4 cut(s) 406, 1228, 2804, 3318
HinP1I GCGC 4 cut(s) 406, 1228, 2804, 3318
HincII GTYRAC 4 cut(s) 457, 2104, 3418, 4348
HindII GTYRAC 4 cut(s) 457, 2104, 3418, 4348
HindIII AAGCTT 2 cut(s) 269, 3201
HpaI GTTAAC 1 cut(s) 4348
HphI GGTGA 6 cut(s) 677, 1559, 1746, 1905, 2407, 3747
Hpy166II GTNNAC 8 cut(s) 457, 1427, 2104, 3418, 3931, 4221, 4320, 4348
Hpy8I GTNNAC 8 cut(s) 457, 1427, 2104, 3418, 3931, 4221, 4320, 4348
Hpy99I CGWCG 2 cut(s) 3367, 4136
HpyCH4III ACNGT 7 cut(s) 64, 1193, 1457, 1903, 2101, 3054, 4013
HpyCH4IV ACGT 4 cut(s) 1429, 1680, 3852, 3975
HpySE526I ACGT 4 cut(s) 1429, 1680, 3852, 3975
Hsp92I GRCGYC 1 cut(s) 2804
HspAI GCGC 4 cut(s) 406, 1228, 2804, 3318
KasI GGCGCC 1 cut(s) 2803
KroI GCCGGC 1 cut(s) 2302
KroNI GCCGGC 1 cut(s) 2304
Ksp22I TGATCA 6 cut(s) 90, 190, 283, 616, 658, 3046
KspAI GTTAAC 1 cut(s) 4348
LguI GCTCTTC 1 cut(s) 1707
LmnI GCTCC 7 cut(s) 490, 547, 685, 2401, 2695, 2759, 2812
MaeII ACGT 4 cut(s) 1429, 1680, 3852, 3975
MbiI CCGCTC 1 cut(s) 3079
MfeI CAATTG 1 cut(s) 2898
MflI RGATCY 4 cut(s) 48, 991, 2465, 2733
MhlI GDGCHC 6 cut(s) 112, 376, 527, 3726, 3917, 4444
MlsI TGGCCA 2 cut(s) 1407, 1536
MluI ACGCGT 1 cut(s) 2475
MluNI TGGCCA 2 cut(s) 1407, 1536
Mly113I GGCGCC 1 cut(s) 2804
MlyI GAGTC 8 cut(s) 354, 641, 814, 986, 1120, 1316, 1745, 2045
MmeI TCCRAC 5 cut(s) 442, 687, 1897, 2182, 4264
Mox20I TGGCCA 2 cut(s) 1407, 1536
Mph1103I ATGCAT 1 cut(s) 3499
MroNI GCCGGC 1 cut(s) 2302
MroXI GAANNNNTTC 4 cut(s) 2643, 2933, 3828, 4330
MscI TGGCCA 2 cut(s) 1407, 1536
MslI CAYNNNNRTG 6 cut(s) 1244, 1401, 1845, 1984, 3096, 3657
Msp20I TGGCCA 2 cut(s) 1407, 1536
MspA1I CMGCKG 4 cut(s) 88, 608, 3428, 3780
MspR9I CCNGG 6 cut(s) 1783, 2508, 3022, 3997, 4037, 4228
MunI CAATTG 1 cut(s) 2898
Mva1269I GAATGC 5 cut(s) 2074, 2762, 3497, 4137, 4404
MvaI CCWGG 3 cut(s) 2508, 3022, 4037
MvnI CGCG 3 cut(s) 721, 2477, 3728
NaeI GCCGGC 1 cut(s) 2304
NarI GGCGCC 1 cut(s) 2804
NciI CCSGG 3 cut(s) 1783, 3997, 4228
NdeI CATATG 1 cut(s) 2675
NgoMIV GCCGGC 1 cut(s) 2302
NlaIV GGNNCC 7 cut(s) 789, 813, 993, 2403, 2805, 3696, 3916
NmeAIII GCCGAG 1 cut(s) 2165
NmuCI GTSAC 4 cut(s) 161, 326, 1838, 2989
NsbI TGCGCA 1 cut(s) 407
NsiI ATGCAT 1 cut(s) 3499
NspI RCATGY 3 cut(s) 1629, 3283, 3744
NspV TTCGAA 3 cut(s) 398, 3008, 3802
PagI TCATGA 2 cut(s) 3345, 3847
PceI AGGCCT 2 cut(s) 655, 3329
PciSI GCTCTTC 1 cut(s) 1707
PcsI WCGNNNNNNNCGW 3 cut(s) 1044, 2112, 3507
PctI GAATGC 5 cut(s) 2074, 2762, 3497, 4137, 4404
PdiI GCCGGC 1 cut(s) 2304
PdmI GAANNNNTTC 4 cut(s) 2643, 2933, 3828, 4330
PflFI GACNNNGTC 1 cut(s) 2545
PflMI CCANNNNNTGG 1 cut(s) 2043
PfoI TCCNGGA 3 cut(s) 1781, 2506, 4035
PleI GAGTC 8 cut(s) 353, 640, 814, 985, 1119, 1316, 1744, 2045
PluTI GGCGCC 1 cut(s) 2807
PpsI GAGTC 8 cut(s) 353, 640, 814, 985, 1119, 1316, 1744, 2045
Ppu21I YACGTR 2 cut(s) 1430, 3976
PpuMI RGGWCCY 1 cut(s) 2146
PshAI GACNNNNGTC 1 cut(s) 2099
PshBI ATTAAT 1 cut(s) 2909
Psp124BI GAGCTC 1 cut(s) 527
Psp5II RGGWCCY 1 cut(s) 2146
Psp6I CCWGG 3 cut(s) 2506, 3020, 4035
PspGI CCWGG 3 cut(s) 2506, 3020, 4035
PspN4I GGNNCC 7 cut(s) 789, 813, 993, 2403, 2805, 3696, 3916
PspPI GGNCC 3 cut(s) 28, 869, 2146
PspPPI RGGWCCY 1 cut(s) 2146
PstI CTGCAG 2 cut(s) 1942, 3040
PstNI CAGNNNCTG 1 cut(s) 2737
PsuI RGATCY 4 cut(s) 48, 991, 2465, 2733
PsyI GACNNNGTC 1 cut(s) 2545
PvuII CAGCTG 3 cut(s) 88, 608, 3428
RseI CAYNNNNRTG 6 cut(s) 1244, 1401, 1845, 1984, 3096, 3657
SacI GAGCTC 1 cut(s) 527
SapI GCTCTTC 1 cut(s) 1707
Sau96I GGNCC 3 cut(s) 28, 869, 2146
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 8 cut(s) 354, 641, 814, 986, 1120, 1316, 1745, 2045
ScrFI CCNGG 6 cut(s) 1783, 2508, 3022, 3997, 4037, 4228
SduI GDGCHC 6 cut(s) 112, 376, 527, 3726, 3917, 4444
SfcI CTRYAG 4 cut(s) 1938, 3036, 3759, 4027
SfoI GGCGCC 1 cut(s) 2805
SfuI TTCGAA 3 cut(s) 398, 3008, 3802
SinI GGWCC 2 cut(s) 869, 2146
SmiMI CAYNNNNRTG 6 cut(s) 1244, 1401, 1845, 1984, 3096, 3657
SmlI CTYRAG 8 cut(s) 469, 908, 1731, 1932, 3029, 3218, 3475, 3485
SmoI CTYRAG 8 cut(s) 469, 908, 1731, 1932, 3029, 3218, 3475, 3485
SnaBI TACGTA 1 cut(s) 3976
SpeI ACTAGT 1 cut(s) 4001
SseBI AGGCCT 2 cut(s) 655, 3329
SspDI GGCGCC 1 cut(s) 2803
SspI AATATT 1 cut(s) 2184
SstI GAGCTC 1 cut(s) 527
StuI AGGCCT 2 cut(s) 655, 3329
StyD4I CCNGG 6 cut(s) 1781, 2506, 3020, 3995, 4035, 4226
StyI CCWWGG 2 cut(s) 1834, 4374
TaaI ACNGT 7 cut(s) 64, 1193, 1457, 1903, 2101, 3054, 4013
TaiI ACGT 4 cut(s) 1432, 1683, 3855, 3978
TatI WGTACW 5 cut(s) 735, 1090, 2323, 3289, 4008
TauI GCSGC 4 cut(s) 724, 1507, 2926, 3082
TscAI CASTG 8 cut(s) 204, 642, 763, 1198, 1537, 1668, 2076, 4212
TseFI GTSAC 4 cut(s) 161, 326, 1838, 2989
Tsp45I GTSAC 4 cut(s) 161, 326, 1838, 2989
TspGWI ACGGA 6 cut(s) 813, 1265, 1731, 1891, 2121, 4365
TspRI CASTG 8 cut(s) 204, 642, 763, 1198, 1537, 1668, 2076, 4212
Tth111I GACNNNGTC 1 cut(s) 2545
Van91I CCANNNNNTGG 1 cut(s) 2043
VpaK11BI GGWCC 2 cut(s) 869, 2146
VspI ATTAAT 1 cut(s) 2909
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 4 cut(s) 946, 2684, 3341, 4428
XceI RCATGY 3 cut(s) 1629, 3283, 3744
XcmI CCANNNNNNNNNTGG 2 cut(s) 338, 4204
XmaJI CCTAGG 1 cut(s) 1834
XmnI GAANNNNTTC 4 cut(s) 2643, 2933, 3828, 4330
ZrmI AGTACT 1 cut(s) 737
Zsp2I ATGCAT 1 cut(s) 3499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.