RchiOBHm_Chr6g0302941

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001838
Physical Location & Seq
Reverse (-)
62845718 .. 62850982
5265 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27214

Sequence Viewer

Length: 4017 bp
ATGGCTAAGAACTATAGATTTTCAATGGACCAAAAGGACATTGTTAGGGTATTGATTACCACAGTAGATGGTTTTATTCGGGATCAGCTGATCAATAAAGAACAGAGAAGCCAACACAGGGAACAGTGTGCTGAAAGATTGGCAGCTGAAGATGGATCCTGTGGTAAAGAAACCGAGGTTCGTTACTCTGATCAAGCAGTGCTAGCAAATCTGGATTGGGGTATTGAGGCTCTTGAAGAAGCAATCGACACTTCAAATATGGAAACTAAGCTTGCAAGATTAGACCATGCTGAAAAAATGTTGCAAGTTTGTGCCATGCTGAATTCTGATCAGAAGACTGCTGGAGTCCCCAATTTTTACCTCTCAGCTTGGGCACACCTCAACTTAGCATACCTGTGGAAATTGAGGAATAACATCCAAAACTCAGTTCTCCATGTCATTGAGATGTTTACAGTTGATCCTTTTTTCTCACGGATAGATTTTGCTCCTGCAATCTGGAAACTTTTGTTTCTTCCACACATGAGCTCAATTATAGGGTGGTATTCGGAGCAGAGGCACCGCCTTGTGATGGAAGTAATTCCTGATTCTCATGATCTGTCTTTCACAGCTGATCTTGATCAATTTTTCAATGAGTCTTTAATCTTTTCAATGAGGCCGGATCAGATAGAGAAATTGCAAAAGCTGGAGCAGCTTTATGGTGAGTCACTGGATGAGAACACAAGGCTTTATGCCAAGTACTTCAAGGATTGCATGACATCTGATTCAACCTCCAGTAAGAAAGTGATTCCAATGTTGCCAATTGCTGAGCCACCAATGACTCCATTGCATGAGGTCAGCCGCTCGATTCCTGATTTTGTTAAGTTTGGTCCTATCTTGCCCAAGAGTGCTGGCTTTTCTCCCATCCTCAAGTCTGAAGATGGCACAAGAGACGAATGTATCCCCGAGGAGAACGAAGATGAATCTGATTATGAGCCAAATGATGCTAATATGGCTTCTGATCACGAGAAGGAAACTGGAGGAAAAGTACAGTCATCTGTACTCAGGAGTCGAGTACATAGCCCAACAATCTTTTCTCCACTAATTTCTCCTAAAACTTCACCAAAAGTTTCATCTCCAAAACCAGACACAGCTGCTTCTGCATTGCGTTTACTGTCTGTCAAAATCACAGATTCAGCTGTTGCCACCTCTTTGCCTGCATCTCCGGGCATGAGCAATGATTACAGCATCAGCTCTGCAGACTCTGATGTTGAAGTGATAGAGACTACCAAAAGTTGCAGGAAAGTCTATAGCCGAACATCAAGCATGAATAATGAGCTTGTGAAAATGTCGAAAAATAGTCCACCTAATGAAAATGATGAAGGAGGCCAGAGCTGTGTTTCACTTCCATCTTCTGAGAGGATGACTACTAAGTCAAGACCACCTAAGGATTTTGTTTGTCCTATCACTGGCCAGATATTTATTGATCCAGTTACTCTTGAAACAGGTCAGACATACGAAAGAAAAGCAATCCAAGAATGGCTGAAACGAGGAAACACAACCTGTCCCATTACGCGGCAACCCATCTCTACAACTACTCAGCTGCCTAAAACAAACTATGTTTTGAAAAGACTGATAACCTCTTGGAAAGAACAGCATCCTGAACTCGCTCAAGAATGTGCATACTACGAAACACCAAGAAACTCATTCCACCGCTCCTCCGTGAAAGAAGTCCATTCTGGTACCACAACTCCACAAAGAACATGTGACTTCATGGGTCATAGGAATACCGATGATTATATCAGCCAAAGGAGCAAAAGGTTTATGCAGGCAGCTGTTTCTACATCACCAACCAGTGTGATATCTCAAGCTGCAGTTGAGACTATTATCAATGGCTTAAAGCCTCATGTTGCATGTCTGTGTAATTCAGATAAACTTCAAGAGTGTGAAACAGCAGTGCTAGAAATAGCCAGATTATGGAAGGACTCAAAGAGTGATCCTGCAATTCATACATTCTTATCGGAGCTGACAACTGTGAATGGATTCATCGAAATACTTTCAGCTTCTCTGAATAGGGAAGTCCTGAGAACATCTATTTACATCCTCTCCGAGCTAATATTTGCCGATGAAAGTGTTGGAGAAACACTTACCAGTGTAGACTCCGACCTTGATTGCCTGGCTGCTCTTTTAAAGAATGGACTAGCTGAGCCTGCTGTTCTCATTTACCAGCTGAGGCCGGTGTTTGCTCAGCTTTCAGCTCATGATCTTATACCCTCCCTTGTGCAGCTAATTCAGAGCAAAAATGAGGAATCAAATGATCTTCAATTACTAATGGACCCCAAAGATGCTGCATTAGCTATTCTTGAGCAAGTCTTGATGGGAGGAGATGAAAATAGCAGATCCATTAATGCTTTGAGTGTTTTTTCTGCGAGTGGTATACCTGCTCTAGTTAAGTGTTTAGATAGGCCAGACGGAAGAAGGTCCATTGTTTCTATTCTATTATGCTGCATGCAAGCTGAAAAAACTTGCAGGAACTTGATAGCAAATAGAATTGAGCTGTCTCCTGTTCTCGAACTATTTCACGCTGGAAGTGATGGTGTGAGAGGCATATGTGTAGAGTTTCTCTCAGAACTGGTACAGTTGAACAGGAGAACATTATGCAATCAGATCTTGCAGACGATTAAAGACGAAGGAGCATTTAGTACAATGCATACCTTTCTTGTATATCTTCAAATGGCTCCAATGGAGCAGCAACCTGCAATTGCTACTCTCCTTCTTCAGCTTGATCTCCTGGTTGAGCCTCGGAAGATGAGCATTTATAGGGAAGAATCTATAGAGGGACTAATTGAGGCACTCCGAAGGAAAGACTTCTCAAACTCTCAAATGATGGCTTTAGATGCCTTGTTATCTCTTACTGGACGTGTGACTTCATCCGGGGATTCATACACTGAAGCCTGGTTACTCAAGATTGCTGGGTTTGATCAACCTTACAATGGTTTGATGAAGGCAGAGTGGCTACGAAAACAAGACAATGATTTGATGGAGACAATGGAAGAAGAAGAGAAAGCAGTAAGCTCTTGGCAGAAAAAAGTTGCTTTTGTACTTTGCAACCATGAGAAGGGATCCATTTTTAAAGCTTTGGAAGAATGCCTCAAGAGTAAATCCATTGAGATGGCAAAGTCATGTCTTGTCATAGCTACATGGCTCACACACATGCTTTCTGTACTTCCTGATACAGGTGTGAAAATCAGTGCTCGCAAGGCCTTGCTTGAAGAATTCATAAATGTTCTCCAATCATCGAATAATTTGGAGGAGAAGATTTTAGCAACCCTGGCTTTGAAATCTTTTGTCAGTGAACCAGCTGCACTTGAAGCACTAGGAGTGTATGCTAAATGCATCTACAAAACATTGAGAAAGCTTAAGAGGAGCACTGTGGTGGCGAGTGACATAATGAAAGCGCTGATGAACTTGTCATCTGTTGACGTAACAGAGTTGTGGAGCTGTGCTGAAGTGGTTGAACTAGATTCAAGCTCAAATGGGGAGGTTACATCTATACTTCATCTAAAAGGCCGTGTTTTAAGCAGCCATTCTGATGGAACTATGAAGGTATGGGATGCTGGCAAGAAAGTATTAAGACTGATTCAAGAAGTCCGCGAGCACACAAAAGCTGTCACATGCCTCTATATTTCACCTTCAGGTGATAAATTATACAGTGGTTCGTTAGACAAAACTATTCGGGTGTGGGCAATCAAAGCAGAAGAAATTCATTGCCTTCAGGTTCACGATGTGAAAGAGGTAGTATATGAGTTGGTTGCAAATGCTAAAGTGGCGTGCTTCATTTCTCAAGGCACTGGAGTGAAGGTTTACGAATGGTCTGGGGTCCAAAAACATGTAAACTTCAACAAATATGTAAAGAGCCTAGCCATGTCAGGGAACAGCCTCTATTGTGGTTGCTCTGGTTACAGCATCCAGGTAAATCAAAATTACGATCAGATAACAGAACTGACTTGTGCTTGTGTTTACAACTTACACAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1338

Amino Acids

149.5

Weight (kDa)

5.64

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 9 - 268 6.4e-75 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 474 - 545 1.5e-15 U-box domain
ARM_LIN_2nd PF23654 620 - 781 2.6e-61 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 782 - 1144 5.9e-116 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR3_1st PF25173 1162 - 1311 3.4e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 1170 - 1246 6.3e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 1174 - 1267 4.3e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 1178 - 1282 6.4e-10 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1181 - 1251 8.6e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1181 - 1272 3.8e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 1183 - 1245 2.6e-08 WDR3 second beta-propeller domain
WD40 PF00400 1205 - 1240 4.7e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1408
Acc36I ACCTGC 2 cut(s) 2425, 2740
Acc65I GGTACC 1 cut(s) 1718
AccB1I GGYRCC 2 cut(s) 555, 1718
AccB7I CCANNNNNTGG 1 cut(s) 1953
AccBSI CCGCTC 2 cut(s) 840, 1692
AccI GTMKAC 2 cut(s) 2133, 2413
AccII CGCG 2 cut(s) 1552, 3630
AciI CCGC 5 cut(s) 559, 838, 1552, 1690, 3628
AcoI YGGCCR 1 cut(s) 1447
AcsI RAATTY 3 cut(s) 322, 3251, 3738
AcuI CTGAAG 7 cut(s) 168, 933, 2738, 2946, 3504, 3654, 3734
AdeI CACNNNGTG 1 cut(s) 3763
AfaI GTAC 9 cut(s) 737, 1026, 1038, 1053, 1720, 2613, 2680, 3078, 3201
AfeI AGCGCT 1 cut(s) 3435
AfiI CCNNNNNNNGG 9 cut(s) 118, 568, 1445, 1551, 1953, 2969, 3094, 3212, 3906
AflII CTTAAG 1 cut(s) 3395
AflIII ACRYGT 3 cut(s) 1739, 2896, 3865
AjiI CACGTC 1 cut(s) 2897
AjnI CCWGG 5 cut(s) 2151, 2766, 2930, 3306, 3945
AjuI GAANNNNNNNTTGG 4 cut(s) 411, 443, 1054, 1086
AleI CACNNNNGTG 2 cut(s) 3410, 3830
Alw21I GWGCWC 4 cut(s) 527, 3232, 3407, 3636
Alw26I GTCTC 5 cut(s) 921, 1253, 1850, 2541, 3014
AlwNI CAGNNNCTG 1 cut(s) 1241
Ama87I CYCGRG 1 cut(s) 941
Aor51HI AGCGCT 1 cut(s) 3435
AoxI GGCC 7 cut(s) 653, 1363, 1447, 2210, 2441, 3237, 3544
ApoI RAATTY 3 cut(s) 322, 3251, 3738
AseI ATTAAT 1 cut(s) 2382
Asp700I GAANNNNTTC 5 cut(s) 576, 2018, 2553, 2843, 3738
Asp718I GGTACC 1 cut(s) 1718
AspLEI GCGC 1 cut(s) 3436
AspS9I GGNCC 5 cut(s) 28, 866, 2311, 2457, 3856
AsuC2I CCSGG 2 cut(s) 1203, 2911
AsuHPI GGTGA 5 cut(s) 710, 1089, 1815, 3657, 3686
AsuNHI GCTAGC 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 941
AvaII GGWCC 5 cut(s) 28, 866, 2311, 2457, 3856
AxyI CCTNAGG 1 cut(s) 1422
BaeGI GKGCMC 1 cut(s) 376
BaeI ACNNNNGTAYC 4 cut(s) 919, 952, 3201, 3234
BalI TGGCCA 1 cut(s) 1449
BamHI GGATCC 2 cut(s) 155, 3098
BanI GGYRCC 2 cut(s) 555, 1718
BanII GRGCYC 1 cut(s) 527
BarI GAAGNNNNNNTAC 2 cut(s) 2919, 2951
BauI CACGAG 1 cut(s) 1001
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 4 cut(s) 527, 3232, 3407, 3636
BbvCI CCTCAGC 1 cut(s) 2207
BceAI ACGGC 1 cut(s) 3531
BciT130I CCWGG 5 cut(s) 2153, 2768, 2932, 3308, 3947
BciVI GTATCC 1 cut(s) 947
BclI TGATCA 6 cut(s) 90, 190, 328, 616, 997, 2956
BcnI CCSGG 2 cut(s) 1203, 2911
BcoDI GTCTC 5 cut(s) 921, 1253, 1850, 2541, 3014
BfaI CTAG 7 cut(s) 203, 1937, 2177, 2423, 3353, 3497, 3896
BfmI CTRYAG 5 cut(s) 13, 1233, 1285, 1848, 2808
BfoI RGCGCY 1 cut(s) 3437
BfrI CTTAAG 1 cut(s) 3395
BfuAI ACCTGC 2 cut(s) 2425, 2740
BfuI GTATCC 1 cut(s) 947
BglII AGATCT 1 cut(s) 2643
BlpI GCTNAGC 3 cut(s) 804, 2181, 2223
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 7 cut(s) 1203, 2153, 2768, 2911, 2932, 3308, 3947
Bme18I GGWCC 5 cut(s) 28, 866, 2311, 2457, 3856
BmeT110I CYCGRG 1 cut(s) 941
BmgBI CACGTC 1 cut(s) 2897
BmgT120I GGNCC 5 cut(s) 28, 866, 2311, 2457, 3856
BmiI GGNNCC 7 cut(s) 157, 557, 1720, 2313, 2715, 3100, 3857
BmrFI CCNGG 7 cut(s) 1203, 2153, 2768, 2911, 2932, 3308, 3947
BmsI GCATC 9 cut(s) 970, 1205, 1233, 1642, 2311, 2863, 3381, 3580, 3951
BmtI GCTAGC 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 341
BplI GAGNNNNNCTC 2 cut(s) 2585, 2617
BpmI CTGGAG 5 cut(s) 363, 704, 754, 1035, 3849
Bpu10I CCTNAGC 1 cut(s) 2207
Bpu1102I GCTNAGC 3 cut(s) 804, 2181, 2223
BpuEI CTTGAG 7 cut(s) 890, 1632, 1827, 2360, 2924, 3113, 3804
BpuMI CCSGG 2 cut(s) 1203, 2911
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 5 cut(s) 174, 942, 2777, 2910, 3306
Bsc4I CCNNNNNNNGG 9 cut(s) 118, 568, 1445, 1551, 1953, 2969, 3094, 3212, 3906
Bse118I RCCGGY 1 cut(s) 2212
Bse21I CCTNAGG 1 cut(s) 1422
Bse3DI GCAATG 4 cut(s) 821, 1139, 1219, 3742
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 5 cut(s) 2153, 2768, 2932, 3308, 3947
BseDI CCNNGG 5 cut(s) 174, 942, 2777, 2910, 3306
BseGI GGATG 9 cut(s) 414, 715, 900, 1404, 1633, 2076, 2906, 3595, 3942
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 9 cut(s) 118, 568, 1445, 1551, 1953, 2969, 3094, 3212, 3906
BseMI GCAATG 4 cut(s) 821, 1139, 1219, 3742
BseRI GAGGAG 5 cut(s) 959, 1684, 2373, 3302, 3415
BseSI GKGCMC 1 cut(s) 376
BseYI CCCAGC 1 cut(s) 2948
BsgI GTGCAG 2 cut(s) 2279, 3324
Bsh1236I CGCG 2 cut(s) 1552, 3630
BshFI GGCC 7 cut(s) 655, 1365, 1449, 2212, 2443, 3239, 3546
BshNI GGYRCC 2 cut(s) 555, 1718
BsiHKAI GWGCWC 4 cut(s) 527, 3232, 3407, 3636
BsiHKCI CYCGRG 1 cut(s) 941
BsiSI CCGG 4 cut(s) 656, 1202, 2213, 2910
BslFI GGGAC 3 cut(s) 332, 1527, 2829
BslI CCNNNNNNNGG 9 cut(s) 118, 568, 1445, 1551, 1953, 2969, 3094, 3212, 3906
BsmAI GTCTC 5 cut(s) 921, 1253, 1850, 2541, 3014
BsmBI CGTCTC 1 cut(s) 921
BsmFI GGGAC 3 cut(s) 332, 1527, 2829
BsmI GAATGC 1 cut(s) 3128
BsnI GGCC 7 cut(s) 655, 1365, 1449, 2212, 2443, 3239, 3546
BsoBI CYCGRG 1 cut(s) 941
Bsp1286I GDGCHC 5 cut(s) 376, 527, 3232, 3407, 3636
Bsp1720I GCTNAGC 3 cut(s) 804, 2181, 2223
BspACI CCGC 5 cut(s) 559, 838, 1552, 1690, 3628
BspANI GGCC 7 cut(s) 655, 1365, 1449, 2212, 2443, 3239, 3546
BspFNI CGCG 2 cut(s) 1552, 3630
BspHI TCATGA 2 cut(s) 589, 2236
BspLI GGNNCC 7 cut(s) 157, 557, 1720, 2313, 2715, 3100, 3857
BspMAI CTGCAG 2 cut(s) 1237, 1852
BspMI ACCTGC 2 cut(s) 2425, 2740
BspOI GCTAGC 1 cut(s) 206
BspT107I GGYRCC 2 cut(s) 555, 1718
BspTI CTTAAG 1 cut(s) 3395
BsrBI CCGCTC 2 cut(s) 840, 1692
BsrDI GCAATG 4 cut(s) 821, 1139, 1219, 3742
BsrFI RCCGGY 1 cut(s) 2212
BssAI RCCGGY 1 cut(s) 2212
BssECI CCNNGG 5 cut(s) 174, 942, 2777, 2910, 3306
BssNAI GTATAC 1 cut(s) 2414
BssSI CACGAG 1 cut(s) 1001
Bst1107I GTATAC 1 cut(s) 2414
Bst2BI CACGAG 1 cut(s) 1001
Bst2UI CCWGG 5 cut(s) 2153, 2768, 2932, 3308, 3947
Bst4CI ACNGT 9 cut(s) 64, 126, 454, 1029, 1152, 2011, 2616, 3409, 3689
Bst6I CTCTTC 1 cut(s) 3030
BstAFI CTTAAG 1 cut(s) 3395
BstENI CCTNNNNNAGG 1 cut(s) 3210
BstF5I GGATG 9 cut(s) 414, 715, 900, 1404, 1633, 2076, 2906, 3595, 3942
BstFNI CGCG 2 cut(s) 1552, 3630
BstH2I RGCGCY 1 cut(s) 3437
BstHHI GCGC 1 cut(s) 3436
BstMAI GTCTC 5 cut(s) 921, 1253, 1850, 2541, 3014
BstNI CCWGG 5 cut(s) 2153, 2768, 2932, 3308, 3947
BstNSI RCATGY 6 cut(s) 1743, 1893, 2488, 3193, 3654, 3869
BstSCI CCNGG 7 cut(s) 1201, 2151, 2766, 2909, 2930, 3306, 3945
BstSFI CTRYAG 5 cut(s) 13, 1233, 1285, 1848, 2808
BstSLI GKGCMC 1 cut(s) 376
BstUI CGCG 2 cut(s) 1552, 3630
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 4 cut(s) 155, 2375, 2643, 3098
BstXI CCANNNNNNTGG 2 cut(s) 3148, 3569
BstYI RGATCY 4 cut(s) 155, 2375, 2643, 3098
BstZ17I GTATAC 1 cut(s) 2414
Bsu36I CCTNAGG 1 cut(s) 1422
BsuI GTATCC 1 cut(s) 947
BsuRI GGCC 7 cut(s) 655, 1365, 1449, 2212, 2443, 3239, 3546
BtrI CACGTC 1 cut(s) 2897
BtsCI GGATG 9 cut(s) 414, 715, 900, 1404, 1633, 2076, 2906, 3595, 3942
BtsI GCAGTG 2 cut(s) 204, 1938
BveI ACCTGC 2 cut(s) 2425, 2740
CaiI CAGNNNCTG 1 cut(s) 1241
CciI TCATGA 2 cut(s) 589, 2236
CfoI GCGC 1 cut(s) 3436
Cfr10I RCCGGY 1 cut(s) 2212
Cfr13I GGNCC 5 cut(s) 28, 866, 2311, 2457, 3856
Csp6I GTAC 9 cut(s) 736, 1025, 1037, 1052, 1719, 2612, 2679, 3077, 3200
CviQI GTAC 9 cut(s) 736, 1025, 1037, 1052, 1719, 2612, 2679, 3077, 3200
DraI TTTAAA 2 cut(s) 2166, 3109
DraIII CACNNNGTG 1 cut(s) 3763
DrdI GACNNNNNNGTC 1 cut(s) 1408
DseDI GACNNNNNNGTC 1 cut(s) 1408
EaeI YGGCCR 1 cut(s) 1447
Eam1104I CTCTTC 1 cut(s) 3030
EarI CTCTTC 1 cut(s) 3030
Ecl136II GAGCTC 1 cut(s) 525
Eco147I AGGCCT 1 cut(s) 3239
Eco24I GRGCYC 1 cut(s) 527
Eco32I GATATC 1 cut(s) 1839
Eco47I GGWCC 5 cut(s) 28, 866, 2311, 2457, 3856
Eco47III AGCGCT 1 cut(s) 3435
Eco53kI GAGCTC 1 cut(s) 525
Eco57I CTGAAG 7 cut(s) 168, 933, 2738, 2946, 3504, 3654, 3734
Eco81I CCTNAGG 1 cut(s) 1422
Eco88I CYCGRG 1 cut(s) 941
EcoICRI GAGCTC 1 cut(s) 525
EcoNI CCTNNNNNAGG 1 cut(s) 3210
EcoRI GAATTC 2 cut(s) 322, 3251
EcoRII CCWGG 5 cut(s) 2151, 2766, 2930, 3306, 3945
EcoRV GATATC 1 cut(s) 1839
EcoT22I ATGCAT 2 cut(s) 2688, 3374
EcoT38I GRGCYC 1 cut(s) 527
Esp3I CGTCTC 1 cut(s) 921
FaqI GGGAC 3 cut(s) 332, 1527, 2829
FauNDI CATATG 1 cut(s) 2585
FbaI TGATCA 6 cut(s) 90, 190, 328, 616, 997, 2956
FblI GTMKAC 2 cut(s) 2133, 2413
FokI GGATG 9 cut(s) 401, 722, 887, 1411, 1620, 2063, 2893, 3602, 3929
FriOI GRGCYC 1 cut(s) 527
FspBI CTAG 7 cut(s) 203, 1937, 2177, 2423, 3353, 3497, 3896
GlaI GCGC 1 cut(s) 3435
GsaI CCCAGC 1 cut(s) 2952
GsuI CTGGAG 5 cut(s) 363, 704, 754, 1035, 3849
HaeII RGCGCY 1 cut(s) 3437
HaeIII GGCC 7 cut(s) 655, 1365, 1449, 2212, 2443, 3239, 3546
HapII CCGG 4 cut(s) 656, 1202, 2213, 2910
HhaI GCGC 1 cut(s) 3436
Hin6I GCGC 1 cut(s) 3434
HinP1I GCGC 1 cut(s) 3434
HincII GTYRAC 1 cut(s) 3457
HindII GTYRAC 1 cut(s) 3457
HindIII AAGCTT 3 cut(s) 269, 3111, 3392
HpaII CCGG 4 cut(s) 656, 1202, 2213, 2910
HphI GGTGA 5 cut(s) 710, 1089, 1815, 3657, 3686
HpyCH4III ACNGT 9 cut(s) 64, 126, 454, 1029, 1152, 2011, 2616, 3409, 3689
HpyCH4IV ACGT 2 cut(s) 2896, 3459
HpySE526I ACGT 2 cut(s) 2896, 3459
HspAI GCGC 1 cut(s) 3434
KpnI GGTACC 1 cut(s) 1722
Ksp22I TGATCA 6 cut(s) 90, 190, 328, 616, 997, 2956
LweI GCATC 9 cut(s) 970, 1205, 1233, 1642, 2311, 2863, 3381, 3580, 3951
MaeI CTAG 7 cut(s) 203, 1937, 2177, 2423, 3353, 3497, 3896
MaeII ACGT 2 cut(s) 2896, 3459
MbiI CCGCTC 2 cut(s) 840, 1692
MfeI CAATTG 2 cut(s) 798, 2736
MflI RGATCY 4 cut(s) 155, 2375, 2643, 3098
MhlI GDGCHC 5 cut(s) 376, 527, 3232, 3407, 3636
MlsI TGGCCA 1 cut(s) 1449
MluNI TGGCCA 1 cut(s) 1449
MlyI GAGTC 8 cut(s) 354, 641, 710, 811, 1054, 1232, 1955, 2129
MmeI TCCRAC 2 cut(s) 2092, 2163
Mox20I TGGCCA 1 cut(s) 1449
Mph1103I ATGCAT 2 cut(s) 2688, 3374
MroXI GAANNNNTTC 5 cut(s) 576, 2018, 2553, 2843, 3738
MscI TGGCCA 1 cut(s) 1449
MslI CAYNNNNRTG 8 cut(s) 394, 443, 1894, 3146, 3188, 3410, 3567, 3830
Msp20I TGGCCA 1 cut(s) 1449
MspA1I CMGCKG 9 cut(s) 88, 146, 608, 1130, 1175, 1579, 1811, 2206, 3338
MspCI CTTAAG 1 cut(s) 3395
MspI CCGG 4 cut(s) 656, 1202, 2213, 2910
MspR9I CCNGG 7 cut(s) 1203, 2153, 2768, 2911, 2932, 3308, 3947
MunI CAATTG 2 cut(s) 798, 2736
Mva1269I GAATGC 1 cut(s) 3128
MvaI CCWGG 5 cut(s) 2153, 2768, 2932, 3308, 3947
MvnI CGCG 2 cut(s) 1552, 3630
NciI CCSGG 2 cut(s) 1203, 2911
NdeI CATATG 1 cut(s) 2585
NheI GCTAGC 1 cut(s) 202
NlaIV GGNNCC 7 cut(s) 157, 557, 1720, 2313, 2715, 3100, 3857
NmuCI GTSAC 5 cut(s) 702, 1742, 2899, 3419, 3646
NsiI ATGCAT 2 cut(s) 2688, 3374
NspI RCATGY 6 cut(s) 1743, 1893, 2488, 3193, 3654, 3869
OliI CACNNNNGTG 2 cut(s) 3410, 3830
PaeI GCATGC 1 cut(s) 2488
PagI TCATGA 2 cut(s) 589, 2236
PceI AGGCCT 1 cut(s) 3239
PciI ACATGT 2 cut(s) 1739, 3865
PctI GAATGC 1 cut(s) 3128
PdmI GAANNNNTTC 5 cut(s) 576, 2018, 2553, 2843, 3738
PflMI CCANNNNNTGG 1 cut(s) 1953
PleI GAGTC 8 cut(s) 353, 640, 709, 811, 1053, 1232, 1955, 2129
PpsI GAGTC 8 cut(s) 353, 640, 709, 811, 1053, 1232, 1955, 2129
PscI ACATGT 2 cut(s) 1739, 3865
PshBI ATTAAT 1 cut(s) 2382
Psp124BI GAGCTC 1 cut(s) 527
Psp6I CCWGG 5 cut(s) 2151, 2766, 2930, 3306, 3945
PspFI CCCAGC 1 cut(s) 2948
PspGI CCWGG 5 cut(s) 2151, 2766, 2930, 3306, 3945
PspN4I GGNNCC 7 cut(s) 157, 557, 1720, 2313, 2715, 3100, 3857
PspPI GGNCC 5 cut(s) 28, 866, 2311, 2457, 3856
PstI CTGCAG 2 cut(s) 1237, 1852
PstNI CAGNNNCTG 1 cut(s) 1241
PsuI RGATCY 4 cut(s) 155, 2375, 2643, 3098
PvuII CAGCTG 9 cut(s) 88, 146, 608, 1130, 1175, 1579, 1811, 2206, 3338
RsaI GTAC 9 cut(s) 737, 1026, 1038, 1053, 1720, 2613, 2680, 3078, 3201
RsaNI GTAC 9 cut(s) 736, 1025, 1037, 1052, 1719, 2612, 2679, 3077, 3200
RseI CAYNNNNRTG 8 cut(s) 394, 443, 1894, 3146, 3188, 3410, 3567, 3830
SacI GAGCTC 1 cut(s) 527
Sau96I GGNCC 5 cut(s) 28, 866, 2311, 2457, 3856
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 8 cut(s) 354, 641, 710, 811, 1054, 1232, 1955, 2129
ScrFI CCNGG 7 cut(s) 1203, 2153, 2768, 2911, 2932, 3308, 3947
SduI GDGCHC 5 cut(s) 376, 527, 3232, 3407, 3636
SfaNI GCATC 9 cut(s) 970, 1205, 1233, 1642, 2311, 2863, 3381, 3580, 3951
SfcI CTRYAG 5 cut(s) 13, 1233, 1285, 1848, 2808
SinI GGWCC 5 cut(s) 28, 866, 2311, 2457, 3856
SmiMI CAYNNNNRTG 8 cut(s) 394, 443, 1894, 3146, 3188, 3410, 3567, 3830
SmlI CTYRAG 8 cut(s) 905, 1647, 1842, 2339, 2939, 3128, 3395, 3819
SmoI CTYRAG 8 cut(s) 905, 1647, 1842, 2339, 2939, 3128, 3395, 3819
SphI GCATGC 1 cut(s) 2488
SseBI AGGCCT 1 cut(s) 3239
SsiI CCGC 5 cut(s) 559, 838, 1552, 1690, 3628
SspI AATATT 1 cut(s) 2094
SspMI CTAG 7 cut(s) 203, 1937, 2177, 2423, 3353, 3497, 3896
SstI GAGCTC 1 cut(s) 527
StuI AGGCCT 1 cut(s) 3239
StyD4I CCNGG 7 cut(s) 1201, 2151, 2766, 2909, 2930, 3306, 3945
TaaI ACNGT 9 cut(s) 64, 126, 454, 1029, 1152, 2011, 2616, 3409, 3689
TaiI ACGT 2 cut(s) 2899, 3462
TaqI TCGA 7 cut(s) 246, 842, 1048, 1328, 2025, 2547, 3275
TatI WGTACW 7 cut(s) 735, 1024, 1036, 1051, 2678, 3076, 3199
TauI GCSGC 2 cut(s) 840, 1555
TseFI GTSAC 5 cut(s) 702, 1742, 2899, 3419, 3646
Tsp45I GTSAC 5 cut(s) 702, 1742, 2899, 3419, 3646
TspGWI ACGGA 3 cut(s) 487, 1687, 2463
Van91I CCANNNNNTGG 1 cut(s) 1953
Vha464I CTTAAG 1 cut(s) 3395
VpaK11BI GGWCC 5 cut(s) 28, 866, 2311, 2457, 3856
VspI ATTAAT 1 cut(s) 2382
XagI CCTNNNNNAGG 1 cut(s) 3210
XapI RAATTY 3 cut(s) 322, 3251, 3738
XceI RCATGY 6 cut(s) 1743, 1893, 2488, 3193, 3654, 3869
XmiI GTMKAC 2 cut(s) 2133, 2413
XmnI GAANNNNTTC 5 cut(s) 576, 2018, 2553, 2843, 3738
XspI CTAG 7 cut(s) 203, 1937, 2177, 2423, 3353, 3497, 3896
ZrmI AGTACT 1 cut(s) 737
Zsp2I ATGCAT 2 cut(s) 2688, 3374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.