MD02G1056600.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001838
Physical Location & Seq
Reverse (-)
4578078 .. 4581985
3908 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1056600.v1.1.491

Sequence Viewer

Length: 2568 bp
ATGCGTTCAACTTCTCCTGACAGTGTTATAATCCAAGGCGGGTTTGAGGGCGTAGTTGGCGAGTTAAGACTTGCCATTAAGAACCTTTGTATGTCAGAAATTCTCAAGGAGTCCGAAATGGCTGTTCTTCAAATTGAGCGGTTCTGGCAGGAGGCAAATGTGGAATGGGATATTCAGAATTTGCTCACGAATCCTCCAGTGATCAACGGTTTTGTGGAGGTTCTTTTCCATTCTGTTGATCCCAGGGTCCTGTTAGCTGCAGTTTTTCTCCTGTCTGAGCTGGGGTCAAGAGACAATGCTGTGATCCAGACGCTAACGCTCGTTAATTCTGATGTCGAACGCATTGTGGCGCTTTTTAAAAAGGGGTTGGTGGAGGCTGTGGTGTTGATATATCTGTTGAGGCATTCGATCCTGAACCTAATTGAGATGGACATGGTGGAGTCTCTCTTACTAGTTATCAAAAGGAAAGGCGATGATTTGCTTAACATGTGTCTAAAGCCGAGAACAGCTGCAGTGGTTTTACTCGGGCTGATTCTTGGTGGCAGTGGAGAGGGTATTGCCTCTTCGATTGTTAACACAGTAGTATCTGAAAAAGCGCTTGAAACAATTATTAGCAGTTTGGAATCTGAATCAATCGAAGAGAGGATTGCAGCAGTTAGGATCTTGTTGAGATGTATGCAGCAATATGGAAGGTGCAGGAACACAATAGCTGATCAAGGTGAGGTGGCTCCTGTTTTGGATAGCTTTGTCGGCGCAAGTGATGGAGAAAAATTTGAGATTGTTCACTTTTTCTTCGAGTTAGTTAAATTAAACAGAAGGACATCTAATGAAAAAATCCTGCATATCATAAAGGATGAAGGCCTTCTCAGCACAATGCACACCCTTCTTATTTATCTGCAGACTGCCCCTCAAGATCAGTGTCCTACTGTGGCCGGTCTACTACTTCAACTCGATCTTCTGGCAGAGCCGAGAAAGATGAGCATATACCGCGAAGAGGCAATAGACATGCTCATCTCATGTCTCAGAAATGCGGACTTCCCTAATGCCCAAATTGCTGCTGCTGAGACAATTATGTCGCTGCAAGGGAGGTTTTCCACATCCGGAAAGCCTCTCACCGGGGCTTTCCTTCTCAAGCGTGCCGGCCTTGACAAAAGTTACAAAAGTCGAGTACGAATGGATCAATTTAGCAATTTTTCTGGAGATGACGAAACTCTTGTTTACTTTTCTGGTCAACATCAAAAGGAGGAAGAGAAGGCGGCTGATAATTGGGAAAGAAAAATGGCATTTGCTTTAGCAAGCCATGAGTTTGGTCTACTTTTCGAGGCTTTAGCAGAAGGCTTGAAGAGCAGAAACGCAGAATTATGTTCAGTGTGCTTCGTGTCAGCCACATGGCTCGTACACATGCTCAGTGTTCTTCCAGACGCAGGAATACGGGGAGCAGCACGGGTCTTCTTGCTCAAACGTTACGTGTCGATATTCAAATCTGCTAAAGATATTGATGACAAAGCTCTCTCTATGCTTGGGTTGAGCAGCTTCATACAAGATCCTGAGGGCATGCAGGATGTAACTTCTAGCATAAAGGATATAGTGAAGGGCCTGAGAGAGCTTAAGAGATTGACTCCCTTGGCATTTCAAATGCTAAAACTCTTCTCTAAGGGACAAGACTCTAGCGCCGAACTGTGGGATCATAAAGAATTAGCTCAAGTCGACTGCAATGAGAATGGAGAGGTTCTTTCAGTCATTTGCTTCGACGATAAAATATTTTCGGGTCACTCCGATGGAACTATAAAGGTATGGACGGGTAAAGGTAGCGTTCTTCATCTCATCCAAGAAATTCGAGAACATATGAAGGCAGTTACTAGCTTGGCAGTTTTGCAATCGGGGGAGGTTTTATATAGTGGTTCACTTGATAGAACTACAAGGGTATGGTCTATTAGCAATGAAGCAATGCACTGTGTAAAAGTACATGATATGAAGGATCAAGTTCATAGTTTAGCCGTCACGAACAGCTTTGCTTACTTCATTCCACAAAGTACTGGTATTAAGGTTCATTCATGGAACGGAAGAACCAAATTGTTAAATTCAAGTAACCATGTAAAGTGCTTTGCTCTAGTACACGGAAAATTATATTGTGGATGCAATGACAGTGGTGTTCAGGAGATTGATTTGGCCACGGGAACACTCAGTACCATTCAAAATGGCACTTGGAAACTACTCACGAAAGCAAACCCTATCCACGTTATTCAAGTTCACAGTGGACTTATATATACAGCCAGTTCTTCCATCGATGGAGCAGCTGTTAAGATATGGAATGCTGCAAACTTCGACATGGTTGGATCGCTTCCGACTACATTGGAAGTGCGGGCGATGGCCATAAGCTCGGAACTAATTTATTTGGGAGGTAAAGGAGGAAGTGTGGAAATATGGGATAGGAATAAGCAGAACAGAATGGACACCCTACAAACTGGTACCAATTGTAAGGTCCTTTGCTTGGCTCTTGATGCCAATGAGGAAGTTTTGGTCGCTGGAACTTCTGATGGCCGAATTTGGGCATGGGGGCTTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

856

Amino Acids

94.32

Weight (kDa)

5.75

Isoelectric Point (pI)

29.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN_2nd PF23654 17 - 175 6.4e-56 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 178 - 551 6.9e-143 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
WD40_Prp19 PF24814 549 - 662 1.1e-08 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 549 - 702 1.6e-10 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 549 - 691 4.2e-07 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 553 - 656 1.8e-06 WDR90/POC16, second beta-propeller
WD40_CDC20-Fz PF24807 555 - 666 2.7e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 560 - 691 9.3e-11 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 573 - 670 1.7e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 585 - 680 2.2e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 585 - 649 4.2e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 585 - 793 4.4e-08 THOC3 beta-propeller domain
WD40_Prp19 PF24814 712 - 851 4.7e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 742 - 853 3.2e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 29
AasI GACNNNNNNGTC 1 cut(s) 1072
Acc65I GGTACC 1 cut(s) 2468
AccB1I GGYRCC 1 cut(s) 2468
AccBSI CCGCTC 1 cut(s) 139
AccI GTMKAC 3 cut(s) 937, 1312, 1707
AccII CGCG 1 cut(s) 990
AccIII TCCGGA 1 cut(s) 1100
AciI CCGC 6 cut(s) 39, 139, 988, 1031, 1256, 2362
AclI AACGTT 1 cut(s) 1462
AclWI GGATC 9 cut(s) 233, 298, 403, 668, 1185, 1538, 1692, 1986, 2344
AcoI YGGCCR 4 cut(s) 930, 2169, 2370, 2539
AcsI RAATTY 6 cut(s) 99, 178, 770, 1833, 2080, 2544
AfaI GTAC 7 cut(s) 1170, 1398, 1965, 2035, 2115, 2188, 2470
AfeI AGCGCT 1 cut(s) 597
AfiI CCNNNNNNNGG 6 cut(s) 994, 1115, 1424, 1680, 2491, 2548
AflII CTTAAG 1 cut(s) 1607
AflIII ACRYGT 2 cut(s) 486, 1467
AhlI ACTAGT 1 cut(s) 451
AjnI CCWGG 1 cut(s) 242
Alw26I GTCTC 4 cut(s) 285, 447, 1025, 1058
AlwI GGATC 9 cut(s) 233, 298, 403, 668, 1185, 1538, 1692, 1986, 2344
Ama87I CYCGRG 1 cut(s) 524
Aor13HI TCCGGA 1 cut(s) 1100
Aor51HI AGCGCT 1 cut(s) 597
AoxI GGCC 7 cut(s) 859, 930, 1141, 1594, 2169, 2370, 2539
ApoI RAATTY 6 cut(s) 99, 178, 770, 1833, 2080, 2544
Asp700I GAANNNNTTC 1 cut(s) 861
Asp718I GGTACC 1 cut(s) 2468
AspLEI GCGC 4 cut(s) 352, 598, 755, 1673
AspS9I GGNCC 3 cut(s) 247, 1594, 2482
AsuC2I CCSGG 1 cut(s) 1117
AsuHPI GGTGA 2 cut(s) 731, 1105
AvaI CYCGRG 1 cut(s) 524
AvaII GGWCC 2 cut(s) 247, 2482
AxyI CCTNAGG 1 cut(s) 1548
BaeI ACNNNNGTAYC 2 cut(s) 567, 600
BalI TGGCCA 2 cut(s) 2171, 2372
BanI GGYRCC 1 cut(s) 2468
BarI GAAGNNNNNNTAC 2 cut(s) 547, 579
BbsI GAAGAC 1 cut(s) 1441
BccI CCATC 7 cut(s) 421, 755, 1772, 2282, 2291, 2362, 2531
BceAI ACGGC 1 cut(s) 1982
BciT130I CCWGG 1 cut(s) 244
BclI TGATCA 2 cut(s) 201, 712
BcnI CCSGG 1 cut(s) 1117
BcoDI GTCTC 4 cut(s) 285, 447, 1025, 1058
BcuI ACTAGT 1 cut(s) 451
BfaI CTAG 5 cut(s) 452, 1572, 1668, 1860, 2111
BfmI CTRYAG 3 cut(s) 258, 510, 896
BfoI RGCGCY 3 cut(s) 353, 599, 1674
BfrI CTTAAG 1 cut(s) 1607
BlpI GCTNAGC 1 cut(s) 2561
BmcAI AGTACT 1 cut(s) 2035
Bme1390I CCNGG 2 cut(s) 244, 1117
Bme18I GGWCC 2 cut(s) 247, 2482
BmeT110I CYCGRG 1 cut(s) 524
BmgT120I GGNCC 3 cut(s) 247, 1594, 2482
BmiI GGNNCC 3 cut(s) 248, 729, 2470
BmrFI CCNGG 2 cut(s) 244, 1117
BmsI GCATC 2 cut(s) 2126, 2491
BpiI GAAGAC 1 cut(s) 1441
BplI GAGNNNNNCTC 2 cut(s) 1603, 1635
BpmI CTGGAG 2 cut(s) 180, 1218
Bpu1102I GCTNAGC 1 cut(s) 2561
BpuEI CTTGAG 4 cut(s) 89, 894, 1115, 1686
BpuMI CCSGG 1 cut(s) 1117
Bsa29I ATCGAT 1 cut(s) 2286
BsaAI YACGTR 1 cut(s) 1468
BsaJI CCNNGG 6 cut(s) 34, 242, 243, 1116, 1623, 2172
BsaWI WCCGGW 1 cut(s) 1100
BsaXI ACNNNNNCTCC 6 cut(s) 178, 208, 2283, 2313, 2400, 2430
Bsc4I CCNNNNNNNGG 6 cut(s) 994, 1115, 1424, 1680, 2491, 2548
Bse118I RCCGGY 2 cut(s) 932, 1139
Bse1I ACTGG 4 cut(s) 197, 2041, 2274, 2470
Bse21I CCTNAGG 1 cut(s) 1548
Bse3DI GCAATG 4 cut(s) 1720, 1945, 1953, 2146
BseAI TCCGGA 1 cut(s) 1100
BseBI CCWGG 1 cut(s) 244
BseCI ATCGAT 1 cut(s) 2286
BseDI CCNNGG 6 cut(s) 34, 242, 243, 1116, 1623, 2172
BseGI GGATG 5 cut(s) 859, 1097, 1567, 1824, 2141
BseLI CCNNNNNNNGG 6 cut(s) 994, 1115, 1424, 1680, 2491, 2548
BseMI GCAATG 4 cut(s) 1720, 1945, 1953, 2146
BseMII CTCAG 8 cut(s) 267, 880, 1036, 1053, 1420, 1539, 1589, 2197
BseNI ACTGG 4 cut(s) 197, 2041, 2274, 2470
BseYI CCCAGC 1 cut(s) 280
BsgI GTGCAG 1 cut(s) 715
Bsh1236I CGCG 1 cut(s) 990
BshFI GGCC 7 cut(s) 861, 932, 1143, 1596, 2171, 2372, 2541
BshNI GGYRCC 1 cut(s) 2468
BshVI ATCGAT 1 cut(s) 2286
BsiHKCI CYCGRG 1 cut(s) 524
BsiSI CCGG 4 cut(s) 933, 1101, 1116, 1140
BslFI GGGAC 1 cut(s) 1671
BslI CCNNNNNNNGG 6 cut(s) 994, 1115, 1424, 1680, 2491, 2548
BsmAI GTCTC 4 cut(s) 285, 447, 1025, 1058
BsmFI GGGAC 1 cut(s) 1671
BsmI GAATGC 2 cut(s) 403, 2317
BsnI GGCC 7 cut(s) 861, 932, 1143, 1596, 2171, 2372, 2541
BsoBI CYCGRG 1 cut(s) 524
Bsp13I TCCGGA 1 cut(s) 1100
Bsp1720I GCTNAGC 1 cut(s) 2561
BspACI CCGC 6 cut(s) 39, 139, 988, 1031, 1256, 2362
BspANI GGCC 7 cut(s) 861, 932, 1143, 1596, 2171, 2372, 2541
BspCNI CTCAG 8 cut(s) 268, 879, 1035, 1054, 1419, 1540, 1590, 2196
BspDI ATCGAT 1 cut(s) 2286
BspEI TCCGGA 1 cut(s) 1100
BspFNI CGCG 1 cut(s) 990
BspLI GGNNCC 3 cut(s) 248, 729, 2470
BspMAI CTGCAG 3 cut(s) 262, 514, 900
BspPI GGATC 9 cut(s) 233, 298, 403, 668, 1185, 1538, 1692, 1986, 2344
BspQI GCTCTTC 1 cut(s) 1337
BspT107I GGYRCC 1 cut(s) 2468
BspTI CTTAAG 1 cut(s) 1607
BsrBI CCGCTC 1 cut(s) 139
BsrDI GCAATG 4 cut(s) 1720, 1945, 1953, 2146
BsrFI RCCGGY 2 cut(s) 932, 1139
BsrI ACTGG 4 cut(s) 197, 2041, 2274, 2470
BssAI RCCGGY 2 cut(s) 932, 1139
BssECI CCNNGG 6 cut(s) 34, 242, 243, 1116, 1623, 2172
BssT1I CCWWGG 2 cut(s) 34, 1623
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 8 cut(s) 23, 209, 580, 928, 1680, 1955, 2147, 2255
Bst6I CTCTTC 6 cut(s) 568, 633, 987, 1242, 1337, 1652
BstAFI CTTAAG 1 cut(s) 1607
BstBAI YACGTR 1 cut(s) 1468
BstC8I GCNNGC 5 cut(s) 1137, 1141, 1297, 1556, 2364
BstDSI CCRYGG 1 cut(s) 2172
BstF5I GGATG 5 cut(s) 859, 1097, 1567, 1824, 2141
BstFNI CGCG 1 cut(s) 990
BstH2I RGCGCY 3 cut(s) 353, 599, 1674
BstHHI GCGC 4 cut(s) 352, 598, 755, 1673
BstMAI GTCTC 4 cut(s) 285, 447, 1025, 1058
BstMWI GCNNNNNNNGC 8 cut(s) 57, 145, 750, 867, 987, 1052, 1344, 2501
BstNI CCWGG 1 cut(s) 244
BstNSI RCATGY 4 cut(s) 490, 1009, 1405, 1558
BstSCI CCNGG 2 cut(s) 242, 1115
BstSFI CTRYAG 3 cut(s) 258, 510, 896
BstUI CGCG 1 cut(s) 990
BstV2I GAAGAC 1 cut(s) 1441
BstX2I RGATCY 2 cut(s) 660, 1543
BstXI CCANNNNNNTGG 1 cut(s) 1307
BstYI RGATCY 2 cut(s) 660, 1543
Bsu15I ATCGAT 1 cut(s) 2286
Bsu36I CCTNAGG 1 cut(s) 1548
BsuRI GGCC 7 cut(s) 861, 932, 1143, 1596, 2171, 2372, 2541
BsuTUI ATCGAT 1 cut(s) 2286
BtgI CCRYGG 1 cut(s) 2172
BtgZI GCGATG 2 cut(s) 486, 2381
BtsCI GGATG 5 cut(s) 859, 1097, 1567, 1824, 2141
BtsI GCAGTG 2 cut(s) 519, 550
Cac8I GCNNGC 5 cut(s) 1137, 1141, 1297, 1556, 2364
CfoI GCGC 4 cut(s) 352, 598, 755, 1673
Cfr10I RCCGGY 2 cut(s) 932, 1139
Cfr13I GGNCC 3 cut(s) 247, 1594, 2482
ClaI ATCGAT 1 cut(s) 2286
CseI GACGC 2 cut(s) 319, 1430
Csp6I GTAC 7 cut(s) 1169, 1397, 1964, 2034, 2114, 2187, 2469
CspCI CAANNNNNGTGG 2 cut(s) 2128, 2163
CviQI GTAC 7 cut(s) 1169, 1397, 1964, 2034, 2114, 2187, 2469
DraI TTTAAA 1 cut(s) 358
DrdI GACNNNNNNGTC 1 cut(s) 1072
DseDI GACNNNNNNGTC 1 cut(s) 1072
EaeI YGGCCR 4 cut(s) 930, 2169, 2370, 2539
Eam1104I CTCTTC 6 cut(s) 568, 633, 987, 1242, 1337, 1652
EarI CTCTTC 6 cut(s) 568, 633, 987, 1242, 1337, 1652
Eco130I CCWWGG 2 cut(s) 34, 1623
Eco147I AGGCCT 1 cut(s) 861
Eco47I GGWCC 2 cut(s) 247, 2482
Eco47III AGCGCT 1 cut(s) 597
Eco81I CCTNAGG 1 cut(s) 1548
Eco88I CYCGRG 1 cut(s) 524
EcoO109I RGGNCCY 3 cut(s) 247, 1594, 2482
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 2 cut(s) 34, 1623
ErhI CCWWGG 2 cut(s) 34, 1623
FaqI GGGAC 1 cut(s) 1671
FauI CCCGC 2 cut(s) 32, 2355
FauNDI CATATG 1 cut(s) 1845
FbaI TGATCA 2 cut(s) 201, 712
FblI GTMKAC 3 cut(s) 937, 1312, 1707
FokI GGATG 5 cut(s) 866, 1084, 1574, 1811, 2148
FspBI CTAG 5 cut(s) 452, 1572, 1668, 1860, 2111
GlaI GCGC 4 cut(s) 351, 597, 754, 1672
GsaI CCCAGC 1 cut(s) 284
GsuI CTGGAG 2 cut(s) 180, 1218
HaeII RGCGCY 3 cut(s) 353, 599, 1674
HaeIII GGCC 7 cut(s) 861, 932, 1143, 1596, 2171, 2372, 2541
HapII CCGG 4 cut(s) 933, 1101, 1116, 1140
HgaI GACGC 2 cut(s) 319, 1430
HhaI GCGC 4 cut(s) 352, 598, 755, 1673
Hin6I GCGC 4 cut(s) 350, 596, 753, 1671
HinP1I GCGC 4 cut(s) 350, 596, 753, 1671
HincII GTYRAC 3 cut(s) 574, 1232, 1708
HindII GTYRAC 3 cut(s) 574, 1232, 1708
HinfI GANTC 8 cut(s) 110, 190, 440, 532, 623, 629, 1618, 1664
HpaI GTTAAC 1 cut(s) 574
HpaII CCGG 4 cut(s) 933, 1101, 1116, 1140
HphI GGTGA 2 cut(s) 731, 1105
Hpy99I CGWCG 1 cut(s) 1754
HpyCH4III ACNGT 8 cut(s) 23, 209, 580, 928, 1680, 1955, 2147, 2255
HpyCH4IV ACGT 3 cut(s) 1462, 1467, 2238
HpyF10VI GCNNNNNNNGC 8 cut(s) 57, 145, 750, 867, 987, 1052, 1344, 2501
HpySE526I ACGT 3 cut(s) 1462, 1467, 2238
HspAI GCGC 4 cut(s) 350, 596, 753, 1671
Kpn2I TCCGGA 1 cut(s) 1100
KpnI GGTACC 1 cut(s) 2472
KroI GCCGGC 1 cut(s) 1139
KroNI GCCGGC 1 cut(s) 1141
Ksp22I TGATCA 2 cut(s) 201, 712
KspAI GTTAAC 1 cut(s) 574
LguI GCTCTTC 1 cut(s) 1337
LmnI GCTCC 3 cut(s) 733, 1436, 2291
LweI GCATC 2 cut(s) 2126, 2491
MaeI CTAG 5 cut(s) 452, 1572, 1668, 1860, 2111
MaeII ACGT 3 cut(s) 1462, 1467, 2238
MaeIII GTNAC 7 cut(s) 1154, 1463, 1564, 1769, 1855, 1999, 2087
MbiI CCGCTC 1 cut(s) 139
MfeI CAATTG 1 cut(s) 2473
MflI RGATCY 2 cut(s) 660, 1543
MlsI TGGCCA 2 cut(s) 2171, 2372
MluNI TGGCCA 2 cut(s) 2171, 2372
MlyI GAGTC 4 cut(s) 119, 449, 1612, 1658
MmeI TCCRAC 2 cut(s) 2314, 2369
Mox20I TGGCCA 2 cut(s) 2171, 2372
MroI TCCGGA 1 cut(s) 1100
MroNI GCCGGC 1 cut(s) 1139
MroXI GAANNNNTTC 1 cut(s) 861
MscI TGGCCA 2 cut(s) 2171, 2372
MslI CAYNNNNRTG 1 cut(s) 1776
Msp20I TGGCCA 2 cut(s) 2171, 2372
MspA1I CMGCKG 2 cut(s) 509, 2297
MspCI CTTAAG 1 cut(s) 1607
MspI CCGG 4 cut(s) 933, 1101, 1116, 1140
MspR9I CCNGG 2 cut(s) 244, 1117
MunI CAATTG 1 cut(s) 2473
Mva1269I GAATGC 2 cut(s) 403, 2317
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 1 cut(s) 990
MwoI GCNNNNNNNGC 8 cut(s) 57, 145, 750, 867, 987, 1052, 1344, 2501
NaeI GCCGGC 1 cut(s) 1141
NciI CCSGG 1 cut(s) 1117
NdeI CATATG 1 cut(s) 1845
NgoMIV GCCGGC 1 cut(s) 1139
NlaIV GGNNCC 3 cut(s) 248, 729, 2470
NmeAIII GCCGAG 2 cut(s) 525, 993
NmuCI GTSAC 2 cut(s) 1769, 1999
NspI RCATGY 4 cut(s) 490, 1009, 1405, 1558
PaeI GCATGC 1 cut(s) 1558
PasI CCCWGGG 1 cut(s) 243
PceI AGGCCT 1 cut(s) 861
PciI ACATGT 1 cut(s) 486
PciSI GCTCTTC 1 cut(s) 1337
PctI GAATGC 2 cut(s) 403, 2317
PdiI GCCGGC 1 cut(s) 1141
PdmI GAANNNNTTC 1 cut(s) 861
PfeI GAWTC 4 cut(s) 190, 532, 623, 629
PleI GAGTC 4 cut(s) 118, 448, 1612, 1658
PpsI GAGTC 4 cut(s) 118, 448, 1612, 1658
Ppu21I YACGTR 1 cut(s) 1468
PpuMI RGGWCCY 2 cut(s) 247, 2482
PscI ACATGT 1 cut(s) 486
PsiI TTATAA 1 cut(s) 29
Psp1406I AACGTT 1 cut(s) 1462
Psp5II RGGWCCY 2 cut(s) 247, 2482
Psp6I CCWGG 1 cut(s) 242
PspFI CCCAGC 1 cut(s) 280
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 3 cut(s) 248, 729, 2470
PspPI GGNCC 3 cut(s) 247, 1594, 2482
PspPPI RGGWCCY 2 cut(s) 247, 2482
PsrI GAACNNNNNNTAC 2 cut(s) 2170, 2202
PstI CTGCAG 3 cut(s) 262, 514, 900
PsuI RGATCY 2 cut(s) 660, 1543
PvuII CAGCTG 2 cut(s) 509, 2297
RsaI GTAC 7 cut(s) 1170, 1398, 1965, 2035, 2115, 2188, 2470
RsaNI GTAC 7 cut(s) 1169, 1397, 1964, 2034, 2114, 2187, 2469
RseI CAYNNNNRTG 1 cut(s) 1776
SalI GTCGAC 1 cut(s) 1706
SapI GCTCTTC 1 cut(s) 1337
Sau96I GGNCC 3 cut(s) 247, 1594, 2482
ScaI AGTACT 1 cut(s) 2035
SchI GAGTC 4 cut(s) 119, 449, 1612, 1658
ScrFI CCNGG 2 cut(s) 244, 1117
SfaNI GCATC 2 cut(s) 2126, 2491
SfcI CTRYAG 3 cut(s) 258, 510, 896
SinI GGWCC 2 cut(s) 247, 2482
SmiMI CAYNNNNRTG 1 cut(s) 1776
SmlI CTYRAG 5 cut(s) 104, 909, 1130, 1607, 1701
SmoI CTYRAG 5 cut(s) 104, 909, 1130, 1607, 1701
SpeI ACTAGT 1 cut(s) 451
SphI GCATGC 1 cut(s) 1558
SseBI AGGCCT 1 cut(s) 861
SsiI CCGC 6 cut(s) 39, 139, 988, 1031, 1256, 2362
SspI AATATT 1 cut(s) 1761
SspMI CTAG 5 cut(s) 452, 1572, 1668, 1860, 2111
StuI AGGCCT 1 cut(s) 861
StyD4I CCNGG 2 cut(s) 242, 1115
StyI CCWWGG 2 cut(s) 34, 1623
TaaI ACNGT 8 cut(s) 23, 209, 580, 928, 1680, 1955, 2147, 2255
TaiI ACGT 3 cut(s) 1465, 1470, 2241
TatI WGTACW 3 cut(s) 1963, 2033, 2113
TauI GCSGC 1 cut(s) 1259
TfiI GAWTC 4 cut(s) 190, 532, 623, 629
TseFI GTSAC 2 cut(s) 1769, 1999
Tsp45I GTSAC 2 cut(s) 1769, 1999
TspGWI ACGGA 2 cut(s) 2076, 2133
Vha464I CTTAAG 1 cut(s) 1607
VpaK11BI GGWCC 2 cut(s) 247, 2482
XapI RAATTY 6 cut(s) 99, 178, 770, 1833, 2080, 2544
XceI RCATGY 4 cut(s) 490, 1009, 1405, 1558
XmiI GTMKAC 3 cut(s) 937, 1312, 1707
XmnI GAANNNNTTC 1 cut(s) 861
XspI CTAG 5 cut(s) 452, 1572, 1668, 1860, 2111
ZrmI AGTACT 1 cut(s) 2035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.