FvH4_2g38380

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001838
Physical Location & Seq
Forward (+)
27780402 .. 27788030
7629 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38380.t3

Sequence Viewer

Length: 4431 bp
ATGGCTAGAAACTATAAATTTTCGATGGACCAGAAGGACATTGCTAAGGTACTGATTACCACGGTAGATGGTTTTATCCGGGATCAGTTGATCAATAAAGAGCAGAGAAGCCAACACAGGGAACAATGTGCTGAGAGATTGGCAGCTGAAGATGGATCATGTGGTAAAGAAACCGAGGTTCGTTACTCCGATCAAGCAGTGCTAGCAAATCTTGATTGGGGCATTGAGGCCCTTGAAGAAGCGATTGACACTTCAAATATGGAAACTAAGCTTGCAAGGCTAGACCATGCTGAAAAAATGTTGCAAGTTTGTGCCATGTTGAATTGTGATCAGAAAACTGCTGGAGTCCCCAACTTTTACCTCTCAGCTTGGGCGCACCTCAACTTAGCATACCTATGGAAACTGAGGAATAACATCCAAAACTCAGTTCTCCATGTCATTGAGATGTTTACAGTAGACCCTTTTTTCTCACGTATAGATTTTGCTCCTGAACTATGGAAACTTCTATTTCTTCCACACATGAGCTCCATTATTGGTTGGTATTCGGAGCAGAGGCAACGGCTTATGATTGAAGTAATTCCTGACTCTCAGGATATGTCTTACACAGCTGATCTTGATCAATTTTTCAATGAGTCTTTAATCTATGCAATGAGGCCGGATCAGATAGATAAATTGCAACAGCTGGAGCAGCTATATGGTGAGTCATTGGATGAGAATACAAGGCTTTATGCCAAGTACTTCAAGGACTGCATGACATCTAATGCGACCTCCAGTAGGAAAGTGATGCCAATGATGCCAATTGCTGAGCCACCAATGACTCCATTGCATGAGGTTAGCCGCTCGATTCCTGATTTTGTCAAGTTTGGTCCTATCTTGCCCAAGAGTGCTGGCTTTTCTCCCATTCTCAAGTCTAAAGATGGCACAAGAGACGTAAACAGAATGAATATAACTTCTGTATCCATGCAAAATTTGGAGTCTGCAAGATGGGATCCTCAGCAATGTATCCCCGAGGAGAATGAAGATGATTCTGATTATGAGCCTCATGATCCTAATTTGGCTTCTGATCATGAGAAGGATGAAGCTGGAGGAAAAGTACAGTTATCTGTACTTAGGAGTCGAGTACATAGTCCAACAATCTTTTCTCCTCTAATTTCCCCTAAAACTTCTCCAAAAGTTTTAACTCCAAAATCAGACACACATGGTTCTGCATTGCGTTTACTGTCTGTCAGAATCACAGATTCAGCTGTTGCCACCTCTTTGCCTGCTTCTCCGGGAATGAGTAATGACTACAGCATCAGCTCAGCAGACACCGATGTGGAAGTGATAGAGACTACCAAAAGTTGCAGGAAAGTCTATAGTCGAACATCAAGCATAAATAATGAGCTTGTGAAAATGTCGAAAAATAGTCCACCTAATGAAAATGATGAAGGAGGCCAGAGCTGTGTTTCACTTCCATCTTCGGAAAGGATGACTGCTAAATCAAGACCACCTAAAGATTTTGTTTGTCCTATCACAGGCCAGATATTTAGTGATCCAGTCACTCTCGAAACAGGTCAGACATTTGAAAGAAAAGCAATCCAAGAATGGCTGAAACGTGGAAATACAACATGTCCCATGACACGGCAACCTATCTCTACAACTGCTCAACTGCCTAATACAAACTATGTTTTGAAAAGATTGATAACATCTTGGAAAGAACAGCATCCTGAACTTTCTCAAGAATGTGCATACTACGAAACACCTAAAAACTCATTCCAGCGCTCCTCTGTGAAAGAAGTCCCTTCTGGTACCACAACTCCACAAAGAATATGTGACTTCATGGGTCATAGAAACACCGATGATTATATCTGTCAAAGGAGCAAAAGATTTATGCATGCAGTTGTTGCTACATCACCGACCAGTGTGATATCTCAAGCTGCAGTTGAGACAATCATCAATGGCTTAAAGCCTCATGTTGCATGTTTATGTACTTCAGAAAAACTACAAGAATGTGAAACCGCAGTGTTAGAAATCGCCAGATTATGGAAGGACTCAAAAGGTGATCCTGCAATTCATCCATTCTTATCTGAGCCGACCACTGTAAATGGGTTCATCGAAATTCTTTCAGCTTCGATGAATAGGGAAGTCTTGAGAACATCAATATACATCCTCTCAGAGCTAATATTTGCTGATGAGAGTGTTGGAGAAACTCTTACCAGTGTAGACTCTGACCTTGATTGCTTGGCTGCTCTGTTGAAGAACGGTCTAGCTGAGCCTGCTGTCCTTATTTATCAGCTGAGGCCGGTGTTTGCTCAGATTTCTGCTCATGATCTTATACCCTCCCTTGTGCTGCTACTTCAGAGCAAAAATGAGGAATCAGATGATCTTCAATTAGTAATAGACCCTAAAGATGCTGCATTATCTATTCTTGAGCAATTCTTAATGGGAGGAGATGAAAACAGCAGATCTATTAGTGCTTTGAGTGTTATTTCTGCGAATGGTATACCTGTCCTAGTTAAGTATTTAGACAGGCCAGAAGGAAGAAGGTCCATTGTTTCTATTCTATTATGTTGCATGCAAGCCGAAAAAACTTGCAGGAACTTGATAGCACATAGAATTGAGCTGTCTCCTGTTCTTGAACTATTTCACACTGGAACTGACGGAGTGAGAGGCATCTGTGTAGAGTTTCTCTCAGAATTGGTACAGTTGAACAGGAGAACATTATGCAATCAGATACTGCAGATGATTAAAGACGAAGGATCGTTTAGTACAATGCATACCTTTCTTGTATATCTTCAAATGGCTCCAATGGAGCAGCAACCTGCAATTGCTACTCTCCTTCTTCAGCTTGATCTCCTGGTTGAGCCATCCAAGATGAGCATTTATAGGGAAGAATCTATAGAGGGACTAATTGAGTCACTCCGAAGGAAAGAGTTCTCGAACTCTCAAATGATGGCTCTAGATGCCTTGTTATGTCTTACTGGACGTATAACTTCATCCGGAGAATCATACACTGAAGTCTGGTTACTCAAGATTGCAGGGTTTGATCAACCTTACAATGCATTAATGAAAGCAGAGCGGCTAAGGAAAAATGACAATGATTTGATAGAGACAATGGAGGAAGAAGAGAAAGCACTAAGCTCTTGGCAGAAACGGGTTGCTTTTGTACTCTGCAACCATGAGAAGGGATCCATTTTTAAAGCCTTGGAAGAATGCCTCAAGAGTAATTCCATTGAGATGGCAAAATCATGTCTTGTAATAGCTACATGGCTCATACACATGCTTTCTGTACTTCCAGACACTGGTGTGAAAATCAGTGCTCGCAATGCCTTGCTCGAAGAATTGGTAAACGTTCTTCAATCATCGAATAATTTGGAAGAGAAGATTTTAGCAACCTTGGCTTTGAAATCTTTTGTCAGTGAACCAGCTGCACTTGAAGCACTAGGAGTATACGCTAAATGCATCTACAAGACGTTGCGAAAGCTCAAGAGAAGCACTATGGTGGCGAGTGACATAATGAAAGCCTTGATGAACTTGTCATCTGTTGACGTAAAAGAGTTGTGGAGTTGTGCTGAAGTGGTTGAACTAGATTCATGCTCAAATGGGGAGGTTACTTCTATGCTTCATCTAAAAGGGCGCGTTTTAAGCAGCCATTCTGATGGAACTATAAAGGTATGGGATGCTGGCAAGAAGGCGTTAAGACTGATTCAAGAAGTCCGAGAACACACAAAAGCTGTCACATGCCTCTATATTTCACCTTCAGGTGATAAACTATATAGTGGTTCTTTGGACAAAACAATTCGGGTGTGGGCAATCAAAGCAGAAGAGATCCATTGTCTTCAGGTTCATGATGTGAAAGAGGTAGTCTATAAATTGGTAGCCAATGCTAAAGTGGCGTGCTTCATTTCTCAAGGCACTGGAGTTAGGGTGTATGAATGGTCTGGGGTGCAAAAGCATGTAAACTTCAACAAAAATGTGAAAAGCCTGGCCATGACAGGAAACAACCTCTATTGTGGTTGCTCTGGTTACAGCATCCAGGAGATCGACTTAGGGAGACAAATATCAAGCACATTCTACTCTGGCACAAGAAAGCTGTTGGGAAAACAGGTGTTCTATTCCCTTGAGATTCATGATGGCATTCTATATGCAGGTGGTTCATCAGTCGATGCAACGGCAGGGAAGATATTTTCGCTTCCTAGCAAGGCGGTGTTAGGATCATTCACAACCGGATTCGACATCCACCTCATTGCCATTAACAATGACTTGATCTTTACTGCCACCAAATGTGGGATAATTGAGGTATGGTTGAAAGAGAAGTTCACAAGAATTGCTTCCATCAAAACAGCCAGCGGCGGACACGCTAAGATCACATCCTTAGCGGCAGATATGGATGGAGGATTGCTGTTTGCAGGTTCCTCTGATGGCAGAATCCAGGTTTGGGCACTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1477

Amino Acids

164.42

Weight (kDa)

5.7

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 10 - 268 1.3e-72 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 497 - 568 1.5e-15 U-box domain
ARM_LIN_2nd PF23654 643 - 804 1.5e-61 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 805 - 1167 4.3e-115 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR3_1st PF25173 1193 - 1334 4.2e-12 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 1193 - 1269 7.3e-07 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 1198 - 1290 1.2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 1204 - 1274 2e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 1205 - 1310 5e-11 WDR5 beta-propeller domain
WD40_Prp19 PF24814 1205 - 1292 9.7e-10 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 1207 - 1268 1.3e-08 WDR3 second beta-propeller domain
WD40 PF00400 1228 - 1263 4.8e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4118
Acc36I ACCTGC 3 cut(s) 2809, 4118, 4379
Acc65I GGTACC 1 cut(s) 1787
AccB1I GGYRCC 1 cut(s) 1787
AccB7I CCANNNNNTGG 2 cut(s) 2022, 3281
AccBSI CCGCTC 2 cut(s) 840, 3058
AccI GTMKAC 4 cut(s) 456, 2202, 2482, 3429
AccII CGCG 1 cut(s) 3618
AccIII TCCGGA 1 cut(s) 2978
AciI CCGC 7 cut(s) 838, 1998, 3058, 4184, 4329, 4332, 4358
AclI AACGTT 1 cut(s) 3330
AcoI YGGCCR 1 cut(s) 3966
AcsI RAATTY 3 cut(s) 17, 967, 2097
AcuI CTGAAG 8 cut(s) 168, 1956, 2321, 2807, 3015, 3573, 3723, 3803
AfeI AGCGCT 1 cut(s) 1760
AfiI CCNNNNNNNGG 9 cut(s) 118, 774, 1514, 1620, 2022, 3163, 3281, 4266, 4417
AflIII ACRYGT 1 cut(s) 1607
AjnI CCWGG 4 cut(s) 2835, 3963, 4014, 4410
AjuI GAANNNNNNNTTGG 6 cut(s) 411, 443, 1123, 1155, 4399, 4431
AleI CACNNNNGTG 3 cut(s) 1313, 3284, 3479
Alw21I GWGCWC 2 cut(s) 527, 3301
Alw26I GTCTC 6 cut(s) 921, 1322, 1919, 2610, 3083, 4027
AlwNI CAGNNNCTG 2 cut(s) 2716, 3281
Ama87I CYCGRG 1 cut(s) 1007
Aor13HI TCCGGA 1 cut(s) 2978
Aor51HI AGCGCT 1 cut(s) 1760
AoxI GGCC 7 cut(s) 228, 653, 1432, 1516, 2279, 2510, 3966
ApeKI GCWGC 9 cut(s) 143, 688, 1916, 2225, 2329, 2393, 2794, 3407, 3627
ApoI RAATTY 3 cut(s) 17, 967, 2097
AseI ATTAAT 1 cut(s) 3044
Asp700I GAANNNNTTC 4 cut(s) 576, 2622, 2912, 4309
Asp718I GGTACC 1 cut(s) 1787
AspLEI GCGC 3 cut(s) 376, 1761, 3618
AspS9I GGNCC 4 cut(s) 28, 229, 866, 2526
AsuC2I CCSGG 2 cut(s) 80, 1272
AsuHPI GGTGA 5 cut(s) 710, 1884, 2051, 3726, 3755
AsuNHI GCTAGC 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 1007
AvaII GGWCC 3 cut(s) 28, 866, 2526
BaeGI GKGCMC 1 cut(s) 4423
BaeI ACNNNNGTAYC 2 cut(s) 939, 972
BalI TGGCCA 1 cut(s) 3968
BamHI GGATCC 2 cut(s) 988, 3167
BanI GGYRCC 1 cut(s) 1787
BanII GRGCYC 1 cut(s) 527
BarI GAAGNNNNNNTAC 4 cut(s) 2988, 3020, 3318, 3350
BbsI GAAGAC 1 cut(s) 3809
Bbv12I GWGCWC 2 cut(s) 527, 3301
BbvCI CCTCAGC 2 cut(s) 993, 2276
BbvI GCAGC 9 cut(s) 155, 700, 1903, 2212, 2316, 2380, 2806, 3394, 3639
BceAI ACGGC 3 cut(s) 575, 1637, 4167
BciT130I CCWGG 4 cut(s) 2837, 3965, 4016, 4412
BciVI GTATCC 2 cut(s) 967, 1013
BclI TGATCA 5 cut(s) 90, 328, 616, 1063, 3025
BcnI CCSGG 2 cut(s) 80, 1272
BcoDI GTCTC 6 cut(s) 921, 1322, 1919, 2610, 3083, 4027
BfaI CTAG 9 cut(s) 6, 203, 281, 2246, 2492, 2939, 3422, 3566, 4176
BfmI CTRYAG 5 cut(s) 1288, 1354, 1917, 2717, 2877
BfoI RGCGCY 1 cut(s) 1762
BfuAI ACCTGC 3 cut(s) 2809, 4118, 4379
BfuI GTATCC 2 cut(s) 967, 1013
BglII AGATCT 1 cut(s) 2444
BlpI GCTNAGC 3 cut(s) 804, 1300, 2250
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 6 cut(s) 80, 1272, 2837, 3965, 4016, 4412
Bme18I GGWCC 3 cut(s) 28, 866, 2526
BmeT110I CYCGRG 1 cut(s) 1007
BmgT120I GGNCC 4 cut(s) 28, 229, 866, 2526
BmiI GGNNCC 5 cut(s) 990, 1789, 2784, 3169, 4393
BmrFI CCNGG 6 cut(s) 80, 1272, 2837, 3965, 4016, 4412
BmtI GCTAGC 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 3809
BplI GAGNNNNNCTC 2 cut(s) 2654, 2686
BpmI CTGGAG 5 cut(s) 363, 704, 754, 1104, 3918
Bpu10I CCTNAGC 5 cut(s) 45, 993, 2276, 3062, 4354
Bpu1102I GCTNAGC 3 cut(s) 804, 1300, 2250
BpuMI CCSGG 2 cut(s) 80, 1272
BsaAI YACGTR 1 cut(s) 473
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 5 cut(s) 60, 174, 1008, 3183, 3375
BsaWI WCCGGW 2 cut(s) 2978, 4205
BsaXI ACNNNNNCTCC 8 cut(s) 509, 539, 1077, 1107, 1780, 1810, 3891, 3921
Bsc4I CCNNNNNNNGG 9 cut(s) 118, 774, 1514, 1620, 2022, 3163, 3281, 4266, 4417
Bse118I RCCGGY 1 cut(s) 2281
Bse1I ACTGG 9 cut(s) 771, 1535, 1899, 2196, 2635, 2965, 3286, 3901, 4428
Bse3DI GCAATG 7 cut(s) 39, 654, 821, 1004, 1208, 3310, 4224
Bse8I GATNNNNATC 1 cut(s) 615
BseAI TCCGGA 1 cut(s) 2978
BseBI CCWGG 4 cut(s) 2837, 3965, 4016, 4412
BseDI CCNNGG 5 cut(s) 60, 174, 1008, 3183, 3375
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 9 cut(s) 118, 774, 1514, 1620, 2022, 3163, 3281, 4266, 4417
BseMI GCAATG 7 cut(s) 39, 654, 821, 1004, 1208, 3310, 4224
BseNI ACTGG 9 cut(s) 771, 1535, 1899, 2196, 2635, 2965, 3286, 3901, 4428
BseRI GAGGAG 4 cut(s) 1025, 1134, 1753, 2442
BseSI GKGCMC 1 cut(s) 4423
BseXI GCAGC 9 cut(s) 155, 700, 1903, 2212, 2316, 2380, 2806, 3394, 3639
BsgI GTGCAG 1 cut(s) 3393
Bsh1236I CGCG 1 cut(s) 3618
BshFI GGCC 7 cut(s) 230, 655, 1434, 1518, 2281, 2512, 3968
BshNI GGYRCC 1 cut(s) 1787
BsiHKAI GWGCWC 2 cut(s) 527, 3301
BsiHKCI CYCGRG 1 cut(s) 1007
BsiSI CCGG 6 cut(s) 79, 656, 1271, 2282, 2979, 4206
BslFI GGGAC 4 cut(s) 332, 1596, 1763, 2898
BslI CCNNNNNNNGG 9 cut(s) 118, 774, 1514, 1620, 2022, 3163, 3281, 4266, 4417
BsmAI GTCTC 6 cut(s) 921, 1322, 1919, 2610, 3083, 4027
BsmBI CGTCTC 1 cut(s) 921
BsmFI GGGAC 4 cut(s) 332, 1596, 1763, 2898
BsmI GAATGC 2 cut(s) 3197, 4116
BsnI GGCC 7 cut(s) 230, 655, 1434, 1518, 2281, 2512, 3968
BsoBI CYCGRG 1 cut(s) 1007
Bsp1286I GDGCHC 3 cut(s) 527, 3301, 4423
Bsp13I TCCGGA 1 cut(s) 2978
Bsp1720I GCTNAGC 3 cut(s) 804, 1300, 2250
BspACI CCGC 7 cut(s) 838, 1998, 3058, 4184, 4329, 4332, 4358
BspANI GGCC 7 cut(s) 230, 655, 1434, 1518, 2281, 2512, 3968
BspEI TCCGGA 1 cut(s) 2978
BspFNI CGCG 1 cut(s) 3618
BspHI TCATGA 5 cut(s) 1042, 1066, 2305, 3826, 4108
BspLI GGNNCC 5 cut(s) 990, 1789, 2784, 3169, 4393
BspMAI CTGCAG 2 cut(s) 1921, 2721
BspMI ACCTGC 3 cut(s) 2809, 4118, 4379
BspOI GCTAGC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 1787
BsrBI CCGCTC 2 cut(s) 840, 3058
BsrDI GCAATG 7 cut(s) 39, 654, 821, 1004, 1208, 3310, 4224
BsrFI RCCGGY 1 cut(s) 2281
BsrI ACTGG 9 cut(s) 771, 1535, 1899, 2196, 2635, 2965, 3286, 3901, 4428
BssAI RCCGGY 1 cut(s) 2281
BssECI CCNNGG 5 cut(s) 60, 174, 1008, 3183, 3375
BssNAI GTATAC 2 cut(s) 2483, 3430
BssT1I CCWWGG 2 cut(s) 3183, 3375
Bst1107I GTATAC 2 cut(s) 2483, 3430
Bst2UI CCWGG 4 cut(s) 2837, 3965, 4016, 4412
Bst4CI ACNGT 7 cut(s) 64, 454, 1098, 1221, 2080, 2243, 2685
Bst6I CTCTTC 3 cut(s) 3099, 3351, 3798
BstAPI GCANNNNNTGC 1 cut(s) 1883
BstBAI YACGTR 1 cut(s) 473
BstDSI CCRYGG 1 cut(s) 60
BstENI CCTNNNNNAGG 2 cut(s) 772, 1512
BstFNI CGCG 1 cut(s) 3618
BstH2I RGCGCY 1 cut(s) 1762
BstHHI GCGC 3 cut(s) 376, 1761, 3618
BstMAI GTCTC 6 cut(s) 921, 1322, 1919, 2610, 3083, 4027
BstNI CCWGG 4 cut(s) 2837, 3965, 4016, 4412
BstNSI RCATGY 7 cut(s) 1611, 1877, 1962, 2557, 3262, 3723, 3938
BstSCI CCNGG 6 cut(s) 78, 1270, 2835, 3963, 4014, 4410
BstSFI CTRYAG 5 cut(s) 1288, 1354, 1917, 2717, 2877
BstSLI GKGCMC 1 cut(s) 4423
BstUI CGCG 1 cut(s) 3618
BstV1I GCAGC 9 cut(s) 155, 700, 1903, 2212, 2316, 2380, 2806, 3394, 3639
BstV2I GAAGAC 1 cut(s) 3809
BstX2I RGATCY 4 cut(s) 988, 2444, 3167, 3807
BstXI CCANNNNNNTGG 2 cut(s) 3217, 3638
BstYI RGATCY 4 cut(s) 988, 2444, 3167, 3807
BstZ17I GTATAC 2 cut(s) 2483, 3430
BsuI GTATCC 2 cut(s) 967, 1013
BsuRI GGCC 7 cut(s) 230, 655, 1434, 1518, 2281, 2512, 3968
BtgI CCRYGG 1 cut(s) 60
BtsI GCAGTG 2 cut(s) 204, 2007
BveI ACCTGC 3 cut(s) 2809, 4118, 4379
CaiI CAGNNNCTG 2 cut(s) 2716, 3281
CciI TCATGA 5 cut(s) 1042, 1066, 2305, 3826, 4108
CfoI GCGC 3 cut(s) 376, 1761, 3618
Cfr10I RCCGGY 1 cut(s) 2281
Cfr13I GGNCC 4 cut(s) 28, 229, 866, 2526
DraI TTTAAA 1 cut(s) 3178
EaeI YGGCCR 1 cut(s) 3966
Eam1104I CTCTTC 3 cut(s) 3099, 3351, 3798
EarI CTCTTC 3 cut(s) 3099, 3351, 3798
EciI GGCGGA 1 cut(s) 4347
Ecl136II GAGCTC 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 3183, 3375
Eco24I GRGCYC 1 cut(s) 527
Eco32I GATATC 1 cut(s) 1908
Eco47I GGWCC 3 cut(s) 28, 866, 2526
Eco47III AGCGCT 1 cut(s) 1760
Eco53kI GAGCTC 1 cut(s) 525
Eco57I CTGAAG 8 cut(s) 168, 1956, 2321, 2807, 3015, 3573, 3723, 3803
Eco88I CYCGRG 1 cut(s) 1007
EcoICRI GAGCTC 1 cut(s) 525
EcoNI CCTNNNNNAGG 2 cut(s) 772, 1512
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 4 cut(s) 2835, 3963, 4014, 4410
EcoRV GATATC 1 cut(s) 1908
EcoT14I CCWWGG 2 cut(s) 3183, 3375
EcoT22I ATGCAT 4 cut(s) 1875, 2757, 3043, 3443
EcoT38I GRGCYC 1 cut(s) 527
ErhI CCWWGG 2 cut(s) 3183, 3375
Esp3I CGTCTC 1 cut(s) 921
FalI AAGNNNNNCTT 2 cut(s) 4294, 4326
FaqI GGGAC 4 cut(s) 332, 1596, 1763, 2898
FbaI TGATCA 5 cut(s) 90, 328, 616, 1063, 3025
FblI GTMKAC 4 cut(s) 456, 2202, 2482, 3429
FriOI GRGCYC 1 cut(s) 527
FspBI CTAG 9 cut(s) 6, 203, 281, 2246, 2492, 2939, 3422, 3566, 4176
GlaI GCGC 3 cut(s) 375, 1760, 3617
GsuI CTGGAG 5 cut(s) 363, 704, 754, 1104, 3918
HaeII RGCGCY 1 cut(s) 1762
HaeIII GGCC 7 cut(s) 230, 655, 1434, 1518, 2281, 2512, 3968
HapII CCGG 6 cut(s) 79, 656, 1271, 2282, 2979, 4206
HhaI GCGC 3 cut(s) 376, 1761, 3618
Hin6I GCGC 3 cut(s) 374, 1759, 3616
HinP1I GCGC 3 cut(s) 374, 1759, 3616
HincII GTYRAC 1 cut(s) 3526
HindII GTYRAC 1 cut(s) 3526
HindIII AAGCTT 1 cut(s) 269
HpaII CCGG 6 cut(s) 79, 656, 1271, 2282, 2979, 4206
HphI GGTGA 5 cut(s) 710, 1884, 2051, 3726, 3755
HpyCH4III ACNGT 7 cut(s) 64, 454, 1098, 1221, 2080, 2243, 2685
HpyCH4IV ACGT 7 cut(s) 472, 930, 1594, 2965, 3330, 3452, 3528
HpySE526I ACGT 7 cut(s) 472, 930, 1594, 2965, 3330, 3452, 3528
HspAI GCGC 3 cut(s) 374, 1759, 3616
Kpn2I TCCGGA 1 cut(s) 2978
KpnI GGTACC 1 cut(s) 1791
Ksp22I TGATCA 5 cut(s) 90, 328, 616, 1063, 3025
LmnI GCTCC 8 cut(s) 490, 530, 547, 685, 1766, 1857, 2788, 2791
Lsp1109I GCAGC 9 cut(s) 155, 700, 1903, 2212, 2316, 2380, 2806, 3394, 3639
MaeI CTAG 9 cut(s) 6, 203, 281, 2246, 2492, 2939, 3422, 3566, 4176
MaeII ACGT 7 cut(s) 472, 930, 1594, 2965, 3330, 3452, 3528
MaeIII GTNAC 9 cut(s) 182, 1537, 1811, 2895, 3003, 3488, 3589, 3715, 4004
MbiI CCGCTC 2 cut(s) 840, 3058
MfeI CAATTG 2 cut(s) 798, 2805
MflI RGATCY 4 cut(s) 988, 2444, 3167, 3807
MhlI GDGCHC 3 cut(s) 527, 3301, 4423
MlsI TGGCCA 1 cut(s) 3968
MluNI TGGCCA 1 cut(s) 3968
MmeI TCCRAC 2 cut(s) 1154, 2161
Mox20I TGGCCA 1 cut(s) 3968
Mph1103I ATGCAT 4 cut(s) 1875, 2757, 3043, 3443
MroI TCCGGA 1 cut(s) 2978
MroXI GAANNNNTTC 4 cut(s) 576, 2622, 2912, 4309
MscI TGGCCA 1 cut(s) 3968
MslI CAYNNNNRTG 9 cut(s) 394, 443, 1313, 1963, 3215, 3257, 3284, 3479, 3636
Msp20I TGGCCA 1 cut(s) 3968
MspA1I CMGCKG 7 cut(s) 146, 608, 682, 1244, 2275, 3407, 4329
MspI CCGG 6 cut(s) 79, 656, 1271, 2282, 2979, 4206
MspR9I CCNGG 6 cut(s) 80, 1272, 2837, 3965, 4016, 4412
MunI CAATTG 2 cut(s) 798, 2805
Mva1269I GAATGC 2 cut(s) 3197, 4116
MvaI CCWGG 4 cut(s) 2837, 3965, 4016, 4412
MvnI CGCG 1 cut(s) 3618
NciI CCSGG 2 cut(s) 80, 1272
NheI GCTAGC 1 cut(s) 202
NlaIV GGNNCC 5 cut(s) 990, 1789, 2784, 3169, 4393
NmuCI GTSAC 5 cut(s) 1537, 1811, 2895, 3488, 3715
NsiI ATGCAT 4 cut(s) 1875, 2757, 3043, 3443
NspI RCATGY 7 cut(s) 1611, 1877, 1962, 2557, 3262, 3723, 3938
OliI CACNNNNGTG 3 cut(s) 1313, 3284, 3479
PaeI GCATGC 2 cut(s) 1877, 2557
PagI TCATGA 5 cut(s) 1042, 1066, 2305, 3826, 4108
PaqCI CACCTGC 1 cut(s) 4118
PciI ACATGT 1 cut(s) 1607
PctI GAATGC 2 cut(s) 3197, 4116
PdmI GAANNNNTTC 4 cut(s) 576, 2622, 2912, 4309
PflMI CCANNNNNTGG 2 cut(s) 2022, 3281
PfoI TCCNGGA 3 cut(s) 78, 1270, 4014
Ppu21I YACGTR 1 cut(s) 473
PscI ACATGT 1 cut(s) 1607
PshBI ATTAAT 1 cut(s) 3044
Psp124BI GAGCTC 1 cut(s) 527
Psp1406I AACGTT 1 cut(s) 3330
Psp6I CCWGG 4 cut(s) 2835, 3963, 4014, 4410
PspGI CCWGG 4 cut(s) 2835, 3963, 4014, 4410
PspN4I GGNNCC 5 cut(s) 990, 1789, 2784, 3169, 4393
PspPI GGNCC 4 cut(s) 28, 229, 866, 2526
PstI CTGCAG 2 cut(s) 1921, 2721
PstNI CAGNNNCTG 2 cut(s) 2716, 3281
PsuI RGATCY 4 cut(s) 988, 2444, 3167, 3807
PvuII CAGCTG 6 cut(s) 146, 608, 682, 1244, 2275, 3407
RseI CAYNNNNRTG 9 cut(s) 394, 443, 1313, 1963, 3215, 3257, 3284, 3479, 3636
SacI GAGCTC 1 cut(s) 527
Sau96I GGNCC 4 cut(s) 28, 229, 866, 2526
ScaI AGTACT 1 cut(s) 737
ScrFI CCNGG 6 cut(s) 80, 1272, 2837, 3965, 4016, 4412
SduI GDGCHC 3 cut(s) 527, 3301, 4423
SfcI CTRYAG 5 cut(s) 1288, 1354, 1917, 2717, 2877
SinI GGWCC 3 cut(s) 28, 866, 2526
SmiMI CAYNNNNRTG 9 cut(s) 394, 443, 1313, 1963, 3215, 3257, 3284, 3479, 3636
SphI GCATGC 2 cut(s) 1877, 2557
SsiI CCGC 7 cut(s) 838, 1998, 3058, 4184, 4329, 4332, 4358
SspI AATATT 1 cut(s) 2163
SspMI CTAG 9 cut(s) 6, 203, 281, 2246, 2492, 2939, 3422, 3566, 4176
SstI GAGCTC 1 cut(s) 527
StyD4I CCNGG 6 cut(s) 78, 1270, 2835, 3963, 4014, 4410
StyI CCWWGG 2 cut(s) 3183, 3375
TaaI ACNGT 7 cut(s) 64, 454, 1098, 1221, 2080, 2243, 2685
TaiI ACGT 7 cut(s) 475, 933, 1597, 2968, 3333, 3455, 3531
TatI WGTACW 8 cut(s) 735, 1093, 1105, 1120, 1967, 2747, 3145, 3268
TauI GCSGC 4 cut(s) 840, 3061, 4332, 4361
TseFI GTSAC 5 cut(s) 1537, 1811, 2895, 3488, 3715
TseI GCWGC 9 cut(s) 143, 688, 1916, 2225, 2329, 2393, 2794, 3407, 3627
Tsp45I GTSAC 5 cut(s) 1537, 1811, 2895, 3488, 3715
TspGWI ACGGA 1 cut(s) 2655
Van91I CCANNNNNTGG 2 cut(s) 2022, 3281
VpaK11BI GGWCC 3 cut(s) 28, 866, 2526
VspI ATTAAT 1 cut(s) 3044
XagI CCTNNNNNAGG 2 cut(s) 772, 1512
XapI RAATTY 3 cut(s) 17, 967, 2097
XbaI TCTAGA 1 cut(s) 2938
XceI RCATGY 7 cut(s) 1611, 1877, 1962, 2557, 3262, 3723, 3938
XcmI CCANNNNNNNNNTGG 2 cut(s) 967, 3868
XmiI GTMKAC 4 cut(s) 456, 2202, 2482, 3429
XmnI GAANNNNTTC 4 cut(s) 576, 2622, 2912, 4309
XspI CTAG 9 cut(s) 6, 203, 281, 2246, 2492, 2939, 3422, 3566, 4176
ZrmI AGTACT 1 cut(s) 737
Zsp2I ATGCAT 4 cut(s) 1875, 2757, 3043, 3443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.