Rw6G037430

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001838
Physical Location & Seq
Reverse (-)
61732501 .. 61738461
5961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G037430.1

Sequence Viewer

Length: 4032 bp
ATGGACCAAAAGGACATTGTTAGGGTATTGATTACCACGGTAGATGGTTTTATTCGGGATCAGCTGATCAATAAAGAACAGAGAAGCCAACACAGGGAACAGTGTGCTGAAAGATTGGCAGCTGAAGATGGATCCTGTGGTAAAGAAACCGAGGTTCGTTACTCTGATCAAGCAGTGCTAGCAAATCTGGATTGGGGTATTGAGGCTCTTGAAGAAGCAATCGACACTTCAAATATGGAAACTAAGCTTGCAAGATTAGACCATGCTGAAAAAATGTTGCAAGTTTGTGCCATGCTGAATTCTGATCAGAAGACTGCTGGAGTCCCCAATTTTTACCTCTCAGCTTGGGCACACCTCAACTTAGCATACCTGTGGAAATTGAGGAATAACATCCAAAACTCAGTTCTCCATGTCATTGAGATGTTTACAGTTGATCCTTTTTTCTCACGGATAGATTTTGCTCCTGCAATCTGGAAACTTTTGTTTCTTCCACACATGAGCTCAATTATAGGGTGGTATTCGGAGCAGAGGCACCGGCTTGTGATGGAAGTAATTCCTGATTCTCATGATCTGTCTTTCACTGCTGATCTTGATCAATTTTTCAATGAGTCTTTAATCTTTTCAATGAGGCCGGATCAGATAGAGAAATTGCAAAAGCTGGAGCAGCTTTATGGTGAGTCACTGGATGAGAACACAAGGCTTTATGCCAAGTACTTCAAGGATTGCATGACATCTGATTCAACCTCCAGTAAGAAAGTGATTCCAATGTTGCCAATTGCTGAGCCACCAATGACTCCATTGCATGAGGTCAGCCGCTCGATTCCTGATTTTGTCAAGTTTGGTCCTATCTTGCCCAAGAGTGCTGGCTTTTCTCCCATCCTCAAGTCTAAAGATGGCACAAGAGACGTAAACAGAATGAATCTAAGTTCTGCATCTTCACAAAATTTGGAGCCTGTCAGATGGGATCCTCAGGAATGTATCCCCGAGGAGAACGAAGATGAATCTGATTATGAGCCAAATGATGCTAATATGGCTTCTGATCACGAGAAGGAAACTGGAGGAAAAGTACAGTCATCTGTACTCAGGAGTCGAGTACATAGCCCAACAATCTTTTCTCCACTAATTTCTCCTAAAAATTCACCAAAAGTTTCATCTCCAAAACCAGACACAGCTGCTTCTGTATTGCGTTTACTGTCTGTCAAAATCACAGATTCAGCAGTTGCCACCTCTTTGCCTGCATCTCCGGGCATGAGCAATGATTACAGCATCAGCTCTGCAGACTCTGATGTTGAAGTGATAGAGACTACCAAAAGTTGCAGGAAAGTCTATAGCCGAACATCAAGCTTGAATAATGAGCTTGTGAAAATGTCGAAAAATAGTCCACCTAATGAAAATGATGAAGGAGGCCAGAGCTGTGTTTCACTTCCATCTTCTGACAGGATGACTACTAAGTCAAGACCACCTAAGGATTTTGTTTTTACTCTTGAAACAGGTCAGACATACGAAAGAAAAGCAATCCAAGAATGGCTGAAACGAGGAAACACAACCTGTCCCATTACACGGCAACCCATCTCTACAACTACTCAGCTGCCTAAAACAAACTATGTTTTGAAAAGACTGATAACCTCTTGGAAAGAACAGCATCCTGAACTCGCTCAAGAATGTGCATACTACGAAACACCAAGAAACTCATTCCACCGCTCCTCCGTGAAAGAAGTCCATTCTGGTACCACAACTCCACAAAGAACATGTGACTTCATGGGTCATAGGAATACCGATGATTATATCAGCCAAAGGAGCAAAAGGTTTATGCAGGCAGCTGTTTCTACATCACCAACCAGTGTGATATCTCAAGCTGTAGTTGAGACTATTATCAATGGCTTAAAGCCTCATGTTGCATGTCTGTGTACTTCAGATAAACTTCAAGAGTGTGAAACAGCAGTGCTAGAAATAGCCAGATTATGGAAGGACTCAAAGAGTGATCCTGCAATTCATACATTCTTATCGGAGCTGACAACTGTGAATGGATTCATTGAAATACTTTCAGCTTCTCTGAATAGGGAAGTCCTGAGAACATCTATTTACATCCTCTCCGAGCTAATATTTGCCGATGAAAGTGTTGGAGAAACACTTACCAGTGTAGACTCCGACCTTGATTGCCTGGCTGCTCTTTTAAAGAATGGACTAGCTGAGCCTGCTGTTCTCATTTACCAGCTGAGGCCGGTGTTTGCTCAGCTTTCAGCTCATGATCTTATACCCTCCCTTGTGCAGCTAATTCAGAGCAAAAATGAGGAATCAAATGATCTTCAATTACTAATGGACCCCAAAGATGCTGCATTAGCTATTCTTGAGCAAGTCTTGATGGGAGGAGATGAAAATAGCAGATCCATTAATGCTTTGAGTGTTTTTTCTGCGAATGGTATACCTGCTCTAGTTAAGTGTTTAGATAGGCCAGAAGGAAGAAGGTCCATTGTTTCTATTCTATTATGCTGCATGCAAGCTGACAAAACTTGCAGGAACTTGATAGCAAATAGAATTGAGCTGTCTCCTGTTCTCGAACTATTTCACGCTGGAAGTGATGGTGTGAGAGGCATATGTGTAGAGTTTCTCTCAGAATTGGTACAGTTGAACAGGAGAACATTATGCAATCAGATCTTGCAGACGATTAAAGACGAAGGAGCATTTAGTACAATGCATACCTTTCTTGTATATCTTCAAATGGCTCCAATGGAGCAGCAACCTGCAATTGCTACTCTCCTTCTTCAGCTTGATCTCCTGGTTGAGCCTTGGAAGATGAGCATTTATAGGGAAGAATCTATAGAGGGACTAATTGAGGCACTCCGAAGGAAAGACTTCTCAAACTCTCAAATGATGGCATTAGATGCCTTGTTATCTCTTACTGGACGTGTGACTTCATCCGGGGATTCATACACTGAAGCCTGGTTACTCAAGATTGCTGGGTTTGATCAACCTTACAATGGTTTGATGAAGGCAGAGTGGCTAAGAAAACAAGACAATGATTTGATGGAGACAATGGAAGAAGAAGAGAAAGCAGTAAGCTCTTGGCAGAAAAAAGTTGCTTTTGTACTTTGCAACCATGAGAAGGGATCCATTTTTAAAGCTTTGGAAGAATGCCTCAAGAGTAAATCCATTGAGATGGCAAAGTCATGTCTTGTCATAGCTACATGGCTCACACACATGCTTTCTGTACTTCCTGATACTGGTGTGAAAATCAGTGCTCGCAAGGCCTTGCTTGAAGAATTCATAAATGTTCTCCAATCATCGAATAATTTGGAGGAGAAGATTTTAGCAACCCTGGCTTTGAAATCTTTTGTCAGTGAACCAGCTGCACTTGAAGCACTAGGAGTGTATGCTAAATGCATCTACAAAACATTGAGAAAGCTTAAGAGGAGCACTGTGGTGGCGAGTGACATAATGAAAGCGCTGATGAACTTGTCATCTGTTGACGTAACAGAGTTGTGGAGCTGTGCTGAAGTGGTTGAACTAGATTCAAGCTCAAATGGGGAGGTTACATCTATACTTCATCTAAAAGGCCGTGTTTTAAGCAGCCATTCTGATGGAACTATGAAGGTATGGGATGCTGGCAAGAAAGTATTAAGACTGATTCAAGAAGTCCGCGAGCACACAAAAGCTGTCACATGCCTCTATATTTCACCTTCAGGTGATAAATTATACAGTGGTTCGTTAGACAAAACTATTCGGGTGTGGGCAATCAAAGCAGAAGAAATTCATTGCCTTCAGGTTCACGATGTGAAAGAGGTAGTATATGAGTTGGTTGCAAATGCTAAAGTGGCGTGCTTCATTTCTCAAGGCACTGGAGTGAAGGTTTACGAATGGTCTGGGGTCCAAAAACATGTAAACTTCAACAAATATGTAAAGAGCCTAGCCATGTCAGGGAACAGCCTCTATTGTGGTTGCTCTGGTTACAGCATCCAGGTAAATCAAAATTACGATCAGATAACAGAACTGACTTGTGCTTGTGTTTATCAACTTACACAAAAGATAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1343

Amino Acids

150.12

Weight (kDa)

5.59

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 1 - 260 6.4e-75 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 497 - 548 8e-08 U-box domain
ARM_LIN_2nd PF23654 623 - 784 1.3e-61 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 785 - 1147 5.9e-116 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR3_1st PF25173 1165 - 1314 3.4e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 1173 - 1249 6.3e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 1177 - 1270 4.3e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 1182 - 1285 6.4e-10 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1184 - 1254 8.8e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1184 - 1275 3.7e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 1186 - 1248 2.6e-08 WDR3 second beta-propeller domain
WD40 PF00400 1208 - 1243 4.8e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1450
Acc36I ACCTGC 2 cut(s) 2434, 2749
Acc65I GGTACC 1 cut(s) 1727
AccB1I GGYRCC 2 cut(s) 531, 1727
AccB7I CCANNNNNTGG 1 cut(s) 1962
AccBSI CCGCTC 2 cut(s) 816, 1701
AccI GTMKAC 2 cut(s) 2142, 2422
AccII CGCG 1 cut(s) 3639
AciI CCGC 3 cut(s) 814, 1699, 3637
AcsI RAATTY 5 cut(s) 298, 943, 1135, 3260, 3747
AcuI CTGAAG 7 cut(s) 144, 1896, 2747, 2955, 3513, 3663, 3743
AdeI CACNNNGTG 1 cut(s) 3772
AfeI AGCGCT 1 cut(s) 3444
AfiI CCNNNNNNNGG 7 cut(s) 94, 1560, 1962, 2978, 3103, 3221, 3915
AflII CTTAAG 1 cut(s) 3404
AflIII ACRYGT 3 cut(s) 1748, 2905, 3874
AjiI CACGTC 1 cut(s) 2906
AjnI CCWGG 5 cut(s) 2160, 2775, 2939, 3315, 3954
AjuI GAANNNNNNNTTGG 4 cut(s) 387, 419, 1096, 1128
AleI CACNNNNGTG 2 cut(s) 3419, 3839
Alw21I GWGCWC 4 cut(s) 503, 3241, 3416, 3645
Alw26I GTCTC 5 cut(s) 897, 1295, 1859, 2550, 3023
AlwNI CAGNNNCTG 1 cut(s) 1283
Ama87I CYCGRG 1 cut(s) 983
Aor51HI AGCGCT 1 cut(s) 3444
AoxI GGCC 6 cut(s) 629, 1405, 2219, 2450, 3246, 3553
ApoI RAATTY 5 cut(s) 298, 943, 1135, 3260, 3747
AseI ATTAAT 1 cut(s) 2391
Asp700I GAANNNNTTC 5 cut(s) 552, 2027, 2562, 2852, 3747
Asp718I GGTACC 1 cut(s) 1727
AspLEI GCGC 1 cut(s) 3445
AspS9I GGNCC 5 cut(s) 4, 842, 2320, 2466, 3865
AsuC2I CCSGG 2 cut(s) 1245, 2920
AsuHPI GGTGA 5 cut(s) 686, 1131, 1824, 3666, 3695
AsuNHI GCTAGC 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 983
AvaII GGWCC 5 cut(s) 4, 842, 2320, 2466, 3865
AxyI CCTNAGG 2 cut(s) 969, 1464
BaeGI GKGCMC 1 cut(s) 352
BaeI ACNNNNGTAYC 2 cut(s) 3210, 3243
BamHI GGATCC 3 cut(s) 131, 964, 3107
BanI GGYRCC 2 cut(s) 531, 1727
BanII GRGCYC 1 cut(s) 503
BarI GAAGNNNNNNTAC 2 cut(s) 2928, 2960
BauI CACGAG 1 cut(s) 1043
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 4 cut(s) 503, 3241, 3416, 3645
BbvCI CCTCAGC 1 cut(s) 2216
BceAI ACGGC 2 cut(s) 1577, 3540
BciT130I CCWGG 5 cut(s) 2162, 2777, 2941, 3317, 3956
BciVI GTATCC 1 cut(s) 989
BclI TGATCA 6 cut(s) 66, 166, 304, 592, 1039, 2965
BcnI CCSGG 2 cut(s) 1245, 2920
BcoDI GTCTC 5 cut(s) 897, 1295, 1859, 2550, 3023
BfaI CTAG 7 cut(s) 179, 1946, 2186, 2432, 3362, 3506, 3905
BfmI CTRYAG 4 cut(s) 1275, 1327, 1857, 2817
BfoI RGCGCY 1 cut(s) 3446
BfrI CTTAAG 1 cut(s) 3404
BfuAI ACCTGC 2 cut(s) 2434, 2749
BfuI GTATCC 1 cut(s) 989
BglII AGATCT 1 cut(s) 2652
BlpI GCTNAGC 3 cut(s) 780, 2190, 2232
BmcAI AGTACT 1 cut(s) 713
Bme1390I CCNGG 7 cut(s) 1245, 2162, 2777, 2920, 2941, 3317, 3956
Bme18I GGWCC 5 cut(s) 4, 842, 2320, 2466, 3865
BmeT110I CYCGRG 1 cut(s) 983
BmgBI CACGTC 1 cut(s) 2906
BmgT120I GGNCC 5 cut(s) 4, 842, 2320, 2466, 3865
BmiI GGNNCC 9 cut(s) 133, 533, 951, 966, 1729, 2322, 2724, 3109, 3866
BmrFI CCNGG 7 cut(s) 1245, 2162, 2777, 2920, 2941, 3317, 3956
BmtI GCTAGC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 2594, 2626
BpmI CTGGAG 5 cut(s) 339, 680, 730, 1077, 3858
Bpu10I CCTNAGC 1 cut(s) 2216
Bpu1102I GCTNAGC 3 cut(s) 780, 2190, 2232
BpuEI CTTGAG 7 cut(s) 866, 1641, 1836, 2369, 2933, 3122, 3813
BpuMI CCSGG 2 cut(s) 1245, 2920
BsaBI GATNNNNATC 1 cut(s) 591
BsaJI CCNNGG 6 cut(s) 36, 150, 984, 2786, 2919, 3315
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 1560, 1962, 2978, 3103, 3221, 3915
Bse118I RCCGGY 2 cut(s) 534, 2221
Bse1I ACTGG 8 cut(s) 687, 747, 1060, 1839, 2136, 2905, 3226, 3841
Bse21I CCTNAGG 2 cut(s) 969, 1464
Bse3DI GCAATG 3 cut(s) 797, 1261, 3751
Bse8I GATNNNNATC 1 cut(s) 591
BseBI CCWGG 5 cut(s) 2162, 2777, 2941, 3317, 3956
BseDI CCNNGG 6 cut(s) 36, 150, 984, 2786, 2919, 3315
BseGI GGATG 9 cut(s) 390, 691, 876, 1446, 1642, 2085, 2915, 3604, 3951
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 7 cut(s) 94, 1560, 1962, 2978, 3103, 3221, 3915
BseMI GCAATG 3 cut(s) 797, 1261, 3751
BseNI ACTGG 8 cut(s) 687, 747, 1060, 1839, 2136, 2905, 3226, 3841
BseRI GAGGAG 5 cut(s) 1001, 1693, 2382, 3311, 3424
BseSI GKGCMC 1 cut(s) 352
BseYI CCCAGC 1 cut(s) 2957
BsgI GTGCAG 2 cut(s) 2288, 3333
Bsh1236I CGCG 1 cut(s) 3639
BshFI GGCC 6 cut(s) 631, 1407, 2221, 2452, 3248, 3555
BshNI GGYRCC 2 cut(s) 531, 1727
BsiHKAI GWGCWC 4 cut(s) 503, 3241, 3416, 3645
BsiHKCI CYCGRG 1 cut(s) 983
BsiSI CCGG 5 cut(s) 535, 632, 1244, 2222, 2919
BslFI GGGAC 3 cut(s) 308, 1536, 2838
BslI CCNNNNNNNGG 7 cut(s) 94, 1560, 1962, 2978, 3103, 3221, 3915
BsmAI GTCTC 5 cut(s) 897, 1295, 1859, 2550, 3023
BsmBI CGTCTC 1 cut(s) 897
BsmFI GGGAC 3 cut(s) 308, 1536, 2838
BsmI GAATGC 1 cut(s) 3137
BsnI GGCC 6 cut(s) 631, 1407, 2221, 2452, 3248, 3555
BsoBI CYCGRG 1 cut(s) 983
Bsp1286I GDGCHC 5 cut(s) 352, 503, 3241, 3416, 3645
Bsp1720I GCTNAGC 3 cut(s) 780, 2190, 2232
BspACI CCGC 3 cut(s) 814, 1699, 3637
BspANI GGCC 6 cut(s) 631, 1407, 2221, 2452, 3248, 3555
BspFNI CGCG 1 cut(s) 3639
BspHI TCATGA 2 cut(s) 565, 2245
BspLI GGNNCC 9 cut(s) 133, 533, 951, 966, 1729, 2322, 2724, 3109, 3866
BspMAI CTGCAG 1 cut(s) 1279
BspMI ACCTGC 2 cut(s) 2434, 2749
BspOI GCTAGC 1 cut(s) 182
BspT107I GGYRCC 2 cut(s) 531, 1727
BspTI CTTAAG 1 cut(s) 3404
BsrBI CCGCTC 2 cut(s) 816, 1701
BsrDI GCAATG 3 cut(s) 797, 1261, 3751
BsrFI RCCGGY 2 cut(s) 534, 2221
BsrI ACTGG 8 cut(s) 687, 747, 1060, 1839, 2136, 2905, 3226, 3841
BssAI RCCGGY 2 cut(s) 534, 2221
BssECI CCNNGG 6 cut(s) 36, 150, 984, 2786, 2919, 3315
BssNAI GTATAC 1 cut(s) 2423
BssSI CACGAG 1 cut(s) 1043
BssT1I CCWWGG 1 cut(s) 2786
Bst1107I GTATAC 1 cut(s) 2423
Bst2BI CACGAG 1 cut(s) 1043
Bst2UI CCWGG 5 cut(s) 2162, 2777, 2941, 3317, 3956
Bst4CI ACNGT 9 cut(s) 40, 102, 430, 1071, 1194, 2020, 2625, 3418, 3698
Bst6I CTCTTC 1 cut(s) 3039
BstAFI CTTAAG 1 cut(s) 3404
BstAPI GCANNNNNTGC 1 cut(s) 2882
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 9 cut(s) 390, 691, 876, 1446, 1642, 2085, 2915, 3604, 3951
BstFNI CGCG 1 cut(s) 3639
BstH2I RGCGCY 1 cut(s) 3446
BstHHI GCGC 1 cut(s) 3445
BstMAI GTCTC 5 cut(s) 897, 1295, 1859, 2550, 3023
BstNI CCWGG 5 cut(s) 2162, 2777, 2941, 3317, 3956
BstNSI RCATGY 6 cut(s) 1752, 1902, 2497, 3202, 3663, 3878
BstSCI CCNGG 7 cut(s) 1243, 2160, 2775, 2918, 2939, 3315, 3954
BstSFI CTRYAG 4 cut(s) 1275, 1327, 1857, 2817
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 1 cut(s) 3639
BstV2I GAAGAC 1 cut(s) 317
BstX2I RGATCY 5 cut(s) 131, 964, 2384, 2652, 3107
BstXI CCANNNNNNTGG 2 cut(s) 3157, 3578
BstYI RGATCY 5 cut(s) 131, 964, 2384, 2652, 3107
BstZ17I GTATAC 1 cut(s) 2423
Bsu36I CCTNAGG 2 cut(s) 969, 1464
BsuI GTATCC 1 cut(s) 989
BsuRI GGCC 6 cut(s) 631, 1407, 2221, 2452, 3248, 3555
BtgI CCRYGG 1 cut(s) 36
BtrI CACGTC 1 cut(s) 2906
BtsCI GGATG 9 cut(s) 390, 691, 876, 1446, 1642, 2085, 2915, 3604, 3951
BtsI GCAGTG 3 cut(s) 180, 579, 1947
BveI ACCTGC 2 cut(s) 2434, 2749
CaiI CAGNNNCTG 1 cut(s) 1283
CciI TCATGA 2 cut(s) 565, 2245
CfoI GCGC 1 cut(s) 3445
Cfr10I RCCGGY 2 cut(s) 534, 2221
Cfr13I GGNCC 5 cut(s) 4, 842, 2320, 2466, 3865
DraI TTTAAA 2 cut(s) 2175, 3118
DraIII CACNNNGTG 1 cut(s) 3772
DrdI GACNNNNNNGTC 1 cut(s) 1450
DseDI GACNNNNNNGTC 1 cut(s) 1450
Eam1104I CTCTTC 1 cut(s) 3039
EarI CTCTTC 1 cut(s) 3039
Ecl136II GAGCTC 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 2786
Eco147I AGGCCT 1 cut(s) 3248
Eco24I GRGCYC 1 cut(s) 503
Eco32I GATATC 1 cut(s) 1848
Eco47I GGWCC 5 cut(s) 4, 842, 2320, 2466, 3865
Eco47III AGCGCT 1 cut(s) 3444
Eco53kI GAGCTC 1 cut(s) 501
Eco57I CTGAAG 7 cut(s) 144, 1896, 2747, 2955, 3513, 3663, 3743
Eco81I CCTNAGG 2 cut(s) 969, 1464
Eco88I CYCGRG 1 cut(s) 983
EcoICRI GAGCTC 1 cut(s) 501
EcoRI GAATTC 2 cut(s) 298, 3260
EcoRII CCWGG 5 cut(s) 2160, 2775, 2939, 3315, 3954
EcoRV GATATC 1 cut(s) 1848
EcoT14I CCWWGG 1 cut(s) 2786
EcoT22I ATGCAT 2 cut(s) 2697, 3383
EcoT38I GRGCYC 1 cut(s) 503
ErhI CCWWGG 1 cut(s) 2786
Esp3I CGTCTC 1 cut(s) 897
FaqI GGGAC 3 cut(s) 308, 1536, 2838
FauNDI CATATG 1 cut(s) 2594
FbaI TGATCA 6 cut(s) 66, 166, 304, 592, 1039, 2965
FblI GTMKAC 2 cut(s) 2142, 2422
FokI GGATG 9 cut(s) 377, 698, 863, 1453, 1629, 2072, 2902, 3611, 3938
FriOI GRGCYC 1 cut(s) 503
FspBI CTAG 7 cut(s) 179, 1946, 2186, 2432, 3362, 3506, 3905
GlaI GCGC 1 cut(s) 3444
GsaI CCCAGC 1 cut(s) 2961
GsuI CTGGAG 5 cut(s) 339, 680, 730, 1077, 3858
HaeII RGCGCY 1 cut(s) 3446
HaeIII GGCC 6 cut(s) 631, 1407, 2221, 2452, 3248, 3555
HapII CCGG 5 cut(s) 535, 632, 1244, 2222, 2919
HhaI GCGC 1 cut(s) 3445
Hin6I GCGC 1 cut(s) 3443
HinP1I GCGC 1 cut(s) 3443
HincII GTYRAC 1 cut(s) 3466
HindII GTYRAC 1 cut(s) 3466
HindIII AAGCTT 4 cut(s) 245, 1342, 3120, 3401
HpaII CCGG 5 cut(s) 535, 632, 1244, 2222, 2919
HphI GGTGA 5 cut(s) 686, 1131, 1824, 3666, 3695
HpyCH4III ACNGT 9 cut(s) 40, 102, 430, 1071, 1194, 2020, 2625, 3418, 3698
HpyCH4IV ACGT 3 cut(s) 906, 2905, 3468
HpySE526I ACGT 3 cut(s) 906, 2905, 3468
HspAI GCGC 1 cut(s) 3443
KpnI GGTACC 1 cut(s) 1731
Ksp22I TGATCA 6 cut(s) 66, 166, 304, 592, 1039, 2965
MaeI CTAG 7 cut(s) 179, 1946, 2186, 2432, 3362, 3506, 3905
MaeII ACGT 3 cut(s) 906, 2905, 3468
MbiI CCGCTC 2 cut(s) 816, 1701
MfeI CAATTG 2 cut(s) 774, 2745
MflI RGATCY 5 cut(s) 131, 964, 2384, 2652, 3107
MhlI GDGCHC 5 cut(s) 352, 503, 3241, 3416, 3645
MlyI GAGTC 8 cut(s) 330, 617, 686, 787, 1096, 1274, 1964, 2138
MmeI TCCRAC 2 cut(s) 2101, 2172
Mph1103I ATGCAT 2 cut(s) 2697, 3383
MroXI GAANNNNTTC 5 cut(s) 552, 2027, 2562, 2852, 3747
MslI CAYNNNNRTG 8 cut(s) 370, 419, 1903, 3155, 3197, 3419, 3576, 3839
MspA1I CMGCKG 7 cut(s) 64, 122, 1172, 1588, 1820, 2215, 3347
MspCI CTTAAG 1 cut(s) 3404
MspI CCGG 5 cut(s) 535, 632, 1244, 2222, 2919
MspR9I CCNGG 7 cut(s) 1245, 2162, 2777, 2920, 2941, 3317, 3956
MunI CAATTG 2 cut(s) 774, 2745
Mva1269I GAATGC 1 cut(s) 3137
MvaI CCWGG 5 cut(s) 2162, 2777, 2941, 3317, 3956
MvnI CGCG 1 cut(s) 3639
NciI CCSGG 2 cut(s) 1245, 2920
NdeI CATATG 1 cut(s) 2594
NheI GCTAGC 1 cut(s) 178
NlaIV GGNNCC 9 cut(s) 133, 533, 951, 966, 1729, 2322, 2724, 3109, 3866
NmuCI GTSAC 5 cut(s) 678, 1751, 2908, 3428, 3655
NsiI ATGCAT 2 cut(s) 2697, 3383
NspI RCATGY 6 cut(s) 1752, 1902, 2497, 3202, 3663, 3878
OliI CACNNNNGTG 2 cut(s) 3419, 3839
PaeI GCATGC 1 cut(s) 2497
PagI TCATGA 2 cut(s) 565, 2245
PceI AGGCCT 1 cut(s) 3248
PciI ACATGT 2 cut(s) 1748, 3874
PctI GAATGC 1 cut(s) 3137
PdmI GAANNNNTTC 5 cut(s) 552, 2027, 2562, 2852, 3747
PflMI CCANNNNNTGG 1 cut(s) 1962
PleI GAGTC 8 cut(s) 329, 616, 685, 787, 1095, 1274, 1964, 2138
PpsI GAGTC 8 cut(s) 329, 616, 685, 787, 1095, 1274, 1964, 2138
PscI ACATGT 2 cut(s) 1748, 3874
PshBI ATTAAT 1 cut(s) 2391
Psp124BI GAGCTC 1 cut(s) 503
Psp6I CCWGG 5 cut(s) 2160, 2775, 2939, 3315, 3954
PspFI CCCAGC 1 cut(s) 2957
PspGI CCWGG 5 cut(s) 2160, 2775, 2939, 3315, 3954
PspN4I GGNNCC 9 cut(s) 133, 533, 951, 966, 1729, 2322, 2724, 3109, 3866
PspPI GGNCC 5 cut(s) 4, 842, 2320, 2466, 3865
PstI CTGCAG 1 cut(s) 1279
PstNI CAGNNNCTG 1 cut(s) 1283
PsuI RGATCY 5 cut(s) 131, 964, 2384, 2652, 3107
PvuII CAGCTG 7 cut(s) 64, 122, 1172, 1588, 1820, 2215, 3347
RseI CAYNNNNRTG 8 cut(s) 370, 419, 1903, 3155, 3197, 3419, 3576, 3839
SacI GAGCTC 1 cut(s) 503
Sau96I GGNCC 5 cut(s) 4, 842, 2320, 2466, 3865
ScaI AGTACT 1 cut(s) 713
SchI GAGTC 8 cut(s) 330, 617, 686, 787, 1096, 1274, 1964, 2138
ScrFI CCNGG 7 cut(s) 1245, 2162, 2777, 2920, 2941, 3317, 3956
SduI GDGCHC 5 cut(s) 352, 503, 3241, 3416, 3645
SfcI CTRYAG 4 cut(s) 1275, 1327, 1857, 2817
SinI GGWCC 5 cut(s) 4, 842, 2320, 2466, 3865
SmiMI CAYNNNNRTG 8 cut(s) 370, 419, 1903, 3155, 3197, 3419, 3576, 3839
SmlI CTYRAG 8 cut(s) 881, 1656, 1851, 2348, 2948, 3137, 3404, 3828
SmoI CTYRAG 8 cut(s) 881, 1656, 1851, 2348, 2948, 3137, 3404, 3828
SphI GCATGC 1 cut(s) 2497
SseBI AGGCCT 1 cut(s) 3248
SsiI CCGC 3 cut(s) 814, 1699, 3637
SspI AATATT 1 cut(s) 2103
SspMI CTAG 7 cut(s) 179, 1946, 2186, 2432, 3362, 3506, 3905
SstI GAGCTC 1 cut(s) 503
StuI AGGCCT 1 cut(s) 3248
StyD4I CCNGG 7 cut(s) 1243, 2160, 2775, 2918, 2939, 3315, 3954
StyI CCWWGG 1 cut(s) 2786
TaaI ACNGT 9 cut(s) 40, 102, 430, 1071, 1194, 2020, 2625, 3418, 3698
TaiI ACGT 3 cut(s) 909, 2908, 3471
TaqI TCGA 6 cut(s) 222, 818, 1090, 1370, 2556, 3284
TatI WGTACW 8 cut(s) 711, 1066, 1078, 1093, 1907, 2687, 3085, 3208
TauI GCSGC 1 cut(s) 816
TseFI GTSAC 5 cut(s) 678, 1751, 2908, 3428, 3655
Tsp45I GTSAC 5 cut(s) 678, 1751, 2908, 3428, 3655
TspGWI ACGGA 2 cut(s) 463, 1696
Van91I CCANNNNNTGG 1 cut(s) 1962
Vha464I CTTAAG 1 cut(s) 3404
VpaK11BI GGWCC 5 cut(s) 4, 842, 2320, 2466, 3865
VspI ATTAAT 1 cut(s) 2391
XapI RAATTY 5 cut(s) 298, 943, 1135, 3260, 3747
XceI RCATGY 6 cut(s) 1752, 1902, 2497, 3202, 3663, 3878
XmiI GTMKAC 2 cut(s) 2142, 2422
XmnI GAANNNNTTC 5 cut(s) 552, 2027, 2562, 2852, 3747
XspI CTAG 7 cut(s) 179, 1946, 2186, 2432, 3362, 3506, 3905
ZrmI AGTACT 1 cut(s) 713
Zsp2I ATGCAT 2 cut(s) 2697, 3383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.