Rh2BG059000

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001838
Physical Location & Seq
Reverse (-)
4382519 .. 4389423
6905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG059000.1

Sequence Viewer

Length: 3456 bp
ATGAGTGATGAACACAGCCATGACATGACCACTACTACTAGTTCATCACAGATTCTATGCCACACGGCGGCTTTCGTCTCCGAAACACTTTCGCAATCGGAGCTCCGCCGCCACCTCTTCTCCACGCTCCGCCGCAGGCTCCCGCCACCGTTGGGTTCCAGCCAAACCGCGGCTATTAAATCCCTCAACCTCGCCGCGGAAACCCTAGAAAACGCCATCGCCGAATCCAGCGCAGCCGTCCGGACCTCCTCCCTCCGCCTCTTAACACCAATTTGGGCTGAAGCAGAGAATTCAGTTGAATCGTTCAGCAGGGGCAGCAGTGTAAGAAGAAGACCCAAGTCTCCCTCATTTTATCCTCAAAGAGTTGCACTTGATAATATCTTACCCTCATGGAGTCTGGGAGCAACGCCAAATGTAAATTCAGATTCTGAGCTGGAAGAATCTTCTTTGGAGGACCATTCTCTTGATTCCTTTTTGGATTCCGAAGCTGAAATTGAGGAAAACAACACAAGAATGGAATTATTTGATTCTAGGCGGAACCAAATCCAAAAGCTAAAGCAGCCCATTTCCGCAGAATCAAGTCGTTCATCTCCAAATCCGCTCATGGCAGATTCTGAAAACCAATCTGGTGGTGGAAAATTTACACCTCCTAAGGACTTTGTGTGTCCCATAACTAGTACCCTTTTTGATGATCCAGTGACACTTGAGACTGGTCAGACATATGAGCGAAAAGCCATCCAAGAATGGCTTGATAGAGGAAACTCAACCTGCCCAATTACCCGCCAGAATTTGCAAAGCACCCAACTGCCTAAGACCAACTATGTCCTCAAGCGTCTAATTGCAAGTTGGCAAGAACAGAACCCTGGTATAGCCTTCAGCCCATCTGATAATCCAAGCCCGCAGATTGAGCCAATGGTGAAACCCATAATGCCTTCAACTTCTCCTGTCAGTGTCATAAGCCAAGCTGCTATTGATAGTGCAGTTAGTGAGTTAAGAATTGCCATTACCAATCTTTGTATGTCAGAAATTCTCAAGGAGTCTGAAATGGCTGTTCTTCGTATTGAGCGTTTCTGGCAGGAAGCAAATGTGGAATGGGATATTCAGAATATGCTCTCAAAACCTCCGGTTATTAATGGTTTCGTGGAGGTTCTTTTCAATTCGGTCGATCCTAGAGTTCTAACAGCCACAGTTTTCCTCTTGTCTGAGCTGGGTTCAAGAGACAATGCTGTGATTCAGACACTAACCCAGGTTGACTCTGATGTGGAATGTATTGTGGCTCTTTTCAAAAAGGGGTTGATGGAGGCTGTTGTCTTGATATATCTGCTAAGGCATTGCATTCTTAATCTAGTGGAGATGGACCTGGTGGACTCTCTCTTAATGGTTATTAGAAAGAGAGATGATGATTGGGCTAATATGTGTCTAAAGCCTAAATCAGCTGCAGTGGTTTTACTGGGGCATATTGTTGGCGGCAGTGAATCAATCGCCAACACTGTGACTTCTGAAAAATTACTCGAAAGAATAATCAGCAGTTTGGAATCTGAATCAGCAGAAGAGAAGACTGCAGCGATTGGGATCTTATTAAGATGTATGCAACAGGATGGGCACTGCAGGAACACAATAGCTGATAAAGCTGAGTTGGCTCCTGTTTTGGATAGCTTCACGGGTGCAGAGGATGAAGAACGGTTTGAGATAGTTCTTTTTTTGTCCGAGTTAGTGAAATTAAACAGGAGGACATTCAATGAACAAATCCTGCATATCATAAAGGATGAGGGCCCTTTCAGTACAATGCACACCCTCCTTATTTATCTACAGACTGCTGCTCAAGACCAATGTCCTGTTGTGGCCAGTCTACTACTTCAACTTGATCTTCTGGCAGAGCCGAGAAAGATGAGCATATACCGTGAAGAGGCAATGGACACTCTCATCTCATGTCTCAGAAATATTGAGGTCCCTGCTGCCCAAATCGCTGCTGCTGAGACCATTATGTCACTGCAAGGGACGTTCACCACATCCGGAAAGCCTCTTGCTAGGGCCGTCCTTCTAAAACGTGCTGGCCTTGACAAAATTTATAAACAACGTGTGCGAATGGATCAACTTAGCTACTTTTCTGGAGAAGATGAAACTGTTGAGGAAGAAAAGGCGGCTGATAACTGGGAAAGAAAAATGGCATTTGTTTTAGCAAGCCACGAGTTTGGTCTACTTTTTGAGGCCTTGGCAGAAGGATTGAAGAGCAGAATCTCAGAATTATGTTCTGCATGCTTTGTGTCAGCCACATGGCTCGTACACATGCTCAATGCCCTTCCAGATACAGGAATACGAGGAGCAGCTCGGCTGTGCTTGATTAAACGGTTCATATCAATATTCAAGTCTGCCAAGGATACTGAAGACAAAGCACTTGCTTTGCTGGCGTTGGGCAGCTTCATCCGTGATCCTGAGGGGATGCAAGAATTAAATTCTTGCAGGAGGGATATCGTAAAAGGCCTGAGAGAGCTTAAGAGATACACTCCCTTGGCATTTCAAATGCTAAAAGTCTTTTCCGATGGGCAAGACTCCAGTGCCGAATTGTGGGATCATAAAGAATTAGTTCAAGTAGACTGCAGTGAGAACGGAGAGGTTCTTTCAGTAGTTTGTTTCAAGGATAAAATATTTTCTGGCCATTCAGATGGAACTATAAAGGTGTGGACGGGTAAAGGTAGCATTCTCCATCTAATCCAGGAAATTCGAGAACATACAAAGGCAGTTACTAGCTTGGCAATTTTGCAATCAAAGGAGATTCTATACAGTGGCTCACTAGATAGAACAACAAGGATCTGGTCTATCAGTAATGAAGGAATCCAGTGTGTAGAAGTCCATGATATGAAGGATCAAGTTCATAATCTGGCCGTCACCAATAGTTTTTCATGCTTCATTCCACAAAGCAATGGTATCAAGGCTCACTCATGGAGCGGAGGATCCAAAATGTTAAATTCCAGCAAATCTGTAAAGTGCTTTGCTCTGGTAAATGGGAAAATATATTGTGGATGCCATGATAGCAGTGTTCAGGAGATTGATTTGGTCACAGGAACACTCAGTACCATTCAAACCGGCTCCCGGAAATTACTTGGGAAATCAAATCCTATCCATTGCATTCAGTATCACAGTGGAATGATATATGCAGCAGGTTCTTCGTTGGATGGAGCAGCAGTGAAGATTTGGAGTGCTGCAAACTTCAGTTTGGTTGGGTCTCTGTCAACTACACTAGAAGTGCGGGCTATGGCAATAAGCTCGGAACTGATCTATTTGGGAGGTAAAGGAGGATGTGTGGAAATTTGGGATAGGGAGAAGCAGAACAAAATAATAGACACACTACAAACTGGCACAAACTGTAAGGTTCTTTCCTTGGCTATTGATCCCAATGAGGAAGTTTTGGTCACTGGAACATCTGATGGCCGAATCCAGGCATGGGGACTAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1151

Amino Acids

127.09

Weight (kDa)

5.36

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 217 - 287 4.2e-17 U-box domain
ARM_LIN_2nd PF23654 327 - 484 7.7e-60 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 493 - 846 1.9e-144 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
WD40_Prp19 PF24814 843 - 955 8.3e-09 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 848 - 998 1.2e-07 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 851 - 957 8.9e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 855 - 973 6.1e-09 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 871 - 965 3.1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 874 - 944 6.8e-07 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 879 - 1145 2.4e-15 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 879 - 963 2.4e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 880 - 962 3.6e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 972 - 1149 1.8e-07 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 991 - 1149 6.8e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1002 - 1148 1e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 1051 - 1148 1e-05 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2070
AasI GACNNNNNNGTC 1 cut(s) 1984
Acc36I ACCTGC 2 cut(s) 776, 3149
AccBSI CCGCTC 2 cut(s) 601, 2946
AccI GTMKAC 3 cut(s) 1849, 2197, 2592
AccII CGCG 2 cut(s) 170, 197
AccIII TCCGGA 2 cut(s) 240, 2012
AcoI YGGCCR 4 cut(s) 1842, 2653, 2880, 3427
AcuI CTGAAG 4 cut(s) 300, 859, 2403, 3193
AfaI GTAC 4 cut(s) 679, 1783, 2283, 3073
AflII CTTAAG 1 cut(s) 2492
AflIII ACRYGT 1 cut(s) 2077
AhlI ACTAGT 2 cut(s) 38, 674
AjnI CCWGG 5 cut(s) 862, 1245, 1359, 2712, 3435
AjuI GAANNNNNNNTTGG 2 cut(s) 403, 435
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 7 cut(s) 82, 345, 701, 1212, 1937, 1970, 3228
AlwNI CAGNNNCTG 2 cut(s) 428, 614
Aor13HI TCCGGA 2 cut(s) 240, 2012
AoxI GGCC 9 cut(s) 1771, 1842, 2031, 2053, 2208, 2479, 2653, 2880, 3427
ApaI GGGCCC 1 cut(s) 1775
AseI ATTAAT 1 cut(s) 1131
Asp700I GAANNNNTTC 1 cut(s) 2583
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 7 cut(s) 243, 454, 1357, 1771, 1772, 1948, 2031
AsuC2I CCSGG 1 cut(s) 3091
AsuHPI GGTGA 3 cut(s) 928, 1996, 2878
AvaII GGWCC 4 cut(s) 243, 454, 1357, 1948
AxyI CCTNAGG 2 cut(s) 651, 2433
BaeGI GKGCMC 2 cut(s) 1605, 1775
BalI TGGCCA 2 cut(s) 1844, 2655
BamHI GGATCC 1 cut(s) 2951
BanII GRGCYC 2 cut(s) 105, 1775
BauI CACGAG 1 cut(s) 2186
BbsI GAAGAC 3 cut(s) 337, 1562, 2391
Bbv12I GWGCWC 1 cut(s) 105
BceAI ACGGC 4 cut(s) 81, 221, 2018, 2867
BcgI CGANNNNNNTGC 4 cut(s) 3147, 3181, 3246, 3280
BciT130I CCWGG 5 cut(s) 864, 1247, 1361, 2714, 3437
BciVI GTATCC 1 cut(s) 2371
BcnI CCSGG 1 cut(s) 3091
BcoDI GTCTC 7 cut(s) 82, 345, 701, 1212, 1937, 1970, 3228
BcuI ACTAGT 2 cut(s) 38, 674
BfmI CTRYAG 5 cut(s) 1437, 1560, 1606, 1808, 2596
BfrI CTTAAG 1 cut(s) 2492
BfuAI ACCTGC 2 cut(s) 776, 3149
BfuI GTATCC 1 cut(s) 2371
Bme1390I CCNGG 6 cut(s) 864, 1247, 1361, 2714, 3091, 3437
Bme18I GGWCC 4 cut(s) 243, 454, 1357, 1948
BmgT120I GGNCC 7 cut(s) 243, 454, 1357, 1771, 1772, 1948, 2031
BmiI GGNNCC 8 cut(s) 140, 157, 539, 1641, 1773, 1950, 2953, 3088
BmrFI CCNGG 6 cut(s) 864, 1247, 1361, 2714, 3091, 3437
BmrI ACTGGG 2 cut(s) 1460, 2161
BmsI GCATC 2 cut(s) 2430, 3011
BmuI ACTGGG 2 cut(s) 1460, 2161
BoxI GACNNNNGTC 2 cut(s) 337, 1830
BpiI GAAGAC 3 cut(s) 337, 1562, 2391
BplI GAGNNNNNCTC 2 cut(s) 2488, 2520
BpmI CTGGAG 2 cut(s) 2130, 2536
Bpu10I CCTNAGC 1 cut(s) 1325
BpuEI CTTGAG 4 cut(s) 725, 812, 1016, 1806
BpuMI CCSGG 1 cut(s) 3091
BsaBI GATNNNNATC 1 cut(s) 1571
BsaI GGTCTC 2 cut(s) 1970, 3228
BsaJI CCNNGG 8 cut(s) 168, 195, 862, 1245, 2211, 2373, 2508, 3378
BsaWI WCCGGW 3 cut(s) 240, 1123, 2012
BsaXI ACNNNNNCTCC 6 cut(s) 104, 134, 3168, 3198, 3285, 3315
Bse118I RCCGGY 1 cut(s) 3083
Bse1I ACTGG 9 cut(s) 695, 715, 1455, 1845, 2156, 2553, 2836, 3358, 3418
Bse21I CCTNAGG 2 cut(s) 651, 2433
Bse3DI GCAATG 4 cut(s) 1330, 1917, 2926, 3121
Bse8I GATNNNNATC 1 cut(s) 1571
BseAI TCCGGA 2 cut(s) 240, 2012
BseBI CCWGG 5 cut(s) 864, 1247, 1361, 2714, 3437
BseDI CCNNGG 8 cut(s) 168, 195, 862, 1245, 2211, 2373, 2508, 3378
BseJI GATNNNNATC 1 cut(s) 1571
BseMI GCAATG 4 cut(s) 1330, 1917, 2926, 3121
BseMII CTCAG 9 cut(s) 420, 1194, 1623, 1948, 1965, 2253, 2424, 2474, 3082
BseNI ACTGG 9 cut(s) 695, 715, 1455, 1845, 2156, 2553, 2836, 3358, 3418
BseRI GAGGAG 2 cut(s) 238, 2334
BseSI GKGCMC 2 cut(s) 1605, 1775
BseYI CCCAGC 1 cut(s) 1207
BsgI GTGCAG 2 cut(s) 999, 1686
Bsh1236I CGCG 2 cut(s) 170, 197
Bsh1285I CGRYCG 1 cut(s) 1164
BshFI GGCC 9 cut(s) 1773, 1844, 2033, 2055, 2210, 2481, 2655, 2882, 3429
BsiEI CGRYCG 1 cut(s) 1164
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 5 cut(s) 241, 1124, 2013, 3084, 3091
BslFI GGGAC 3 cut(s) 651, 1934, 2011
BsmAI GTCTC 7 cut(s) 82, 345, 701, 1212, 1937, 1970, 3228
BsmBI CGTCTC 1 cut(s) 82
BsmFI GGGAC 3 cut(s) 651, 1934, 2011
BsmI GAATGC 3 cut(s) 1335, 2697, 3126
BsnI GGCC 9 cut(s) 1773, 1844, 2033, 2055, 2210, 2481, 2655, 2882, 3429
Bso31I GGTCTC 2 cut(s) 1970, 3228
Bsp120I GGGCCC 1 cut(s) 1771
Bsp1286I GDGCHC 3 cut(s) 105, 1605, 1775
Bsp13I TCCGGA 2 cut(s) 240, 2012
BspANI GGCC 9 cut(s) 1773, 1844, 2033, 2055, 2210, 2481, 2655, 2882, 3429
BspCNI CTCAG 9 cut(s) 421, 1195, 1624, 1947, 1966, 2252, 2425, 2475, 3081
BspEI TCCGGA 2 cut(s) 240, 2012
BspFNI CGCG 2 cut(s) 170, 197
BspLI GGNNCC 8 cut(s) 140, 157, 539, 1641, 1773, 1950, 2953, 3088
BspMAI CTGCAG 4 cut(s) 1441, 1564, 1610, 2600
BspMI ACCTGC 2 cut(s) 776, 3149
BspQI GCTCTTC 1 cut(s) 2222
BspTI CTTAAG 1 cut(s) 2492
BspTNI GGTCTC 2 cut(s) 1970, 3228
BsrBI CCGCTC 2 cut(s) 601, 2946
BsrDI GCAATG 4 cut(s) 1330, 1917, 2926, 3121
BsrFI RCCGGY 1 cut(s) 3083
BsrI ACTGG 9 cut(s) 695, 715, 1455, 1845, 2156, 2553, 2836, 3358, 3418
BssAI RCCGGY 1 cut(s) 3083
BssECI CCNNGG 8 cut(s) 168, 195, 862, 1245, 2211, 2373, 2508, 3378
BssSI CACGAG 1 cut(s) 2186
BssT1I CCWWGG 4 cut(s) 2211, 2373, 2508, 3378
Bst2BI CACGAG 1 cut(s) 2186
Bst2UI CCWGG 5 cut(s) 864, 1247, 1361, 2714, 3437
Bst6I CTCTTC 4 cut(s) 122, 1545, 1899, 2222
BstAFI CTTAAG 1 cut(s) 2492
BstC8I GCNNGC 7 cut(s) 137, 899, 2053, 2182, 2257, 2406, 3249
BstDSI CCRYGG 2 cut(s) 168, 195
BstFNI CGCG 2 cut(s) 170, 197
BstHHI GCGC 1 cut(s) 233
BstMAI GTCTC 7 cut(s) 82, 345, 701, 1212, 1937, 1970, 3228
BstMCI CGRYCG 1 cut(s) 1164
BstNI CCWGG 5 cut(s) 864, 1247, 1361, 2714, 3437
BstNSI RCATGY 2 cut(s) 2259, 2290
BstPAI GACNNNNGTC 2 cut(s) 337, 1830
BstSCI CCNGG 6 cut(s) 862, 1245, 1359, 2712, 3089, 3435
BstSFI CTRYAG 5 cut(s) 1437, 1560, 1606, 1808, 2596
BstSLI GKGCMC 2 cut(s) 1605, 1775
BstUI CGCG 2 cut(s) 170, 197
BstV2I GAAGAC 3 cut(s) 337, 1562, 2391
BstX2I RGATCY 3 cut(s) 1572, 2808, 2951
BstXI CCANNNNNNTGG 3 cut(s) 629, 2192, 2663
BstYI RGATCY 3 cut(s) 1572, 2808, 2951
Bsu36I CCTNAGG 2 cut(s) 651, 2433
BsuI GTATCC 1 cut(s) 2371
BsuRI GGCC 9 cut(s) 1773, 1844, 2033, 2055, 2210, 2481, 2655, 2882, 3429
BtgI CCRYGG 2 cut(s) 168, 195
BtgZI GCGATG 1 cut(s) 202
BtsI GCAGTG 8 cut(s) 325, 1446, 1477, 1603, 1988, 2605, 3040, 3189
BveI ACCTGC 2 cut(s) 776, 3149
Cac8I GCNNGC 7 cut(s) 137, 899, 2053, 2182, 2257, 2406, 3249
CaiI CAGNNNCTG 2 cut(s) 428, 614
CfoI GCGC 1 cut(s) 233
Cfr10I RCCGGY 1 cut(s) 3083
Cfr13I GGNCC 7 cut(s) 243, 454, 1357, 1771, 1772, 1948, 2031
Cfr42I CCGCGG 2 cut(s) 171, 198
CseI GACGC 1 cut(s) 821
CsiI ACCWGGT 1 cut(s) 1359
Csp6I GTAC 4 cut(s) 678, 1782, 2282, 3072
CviQI GTAC 4 cut(s) 678, 1782, 2282, 3072
DrdI GACNNNNNNGTC 1 cut(s) 1984
DseDI GACNNNNNNGTC 1 cut(s) 1984
EaeI YGGCCR 4 cut(s) 1842, 2653, 2880, 3427
Eam1104I CTCTTC 4 cut(s) 122, 1545, 1899, 2222
EarI CTCTTC 4 cut(s) 122, 1545, 1899, 2222
EciI GGCGGA 4 cut(s) 95, 119, 245, 550
Ecl136II GAGCTC 1 cut(s) 103
Eco130I CCWWGG 4 cut(s) 2211, 2373, 2508, 3378
Eco147I AGGCCT 2 cut(s) 2210, 2481
Eco24I GRGCYC 2 cut(s) 105, 1775
Eco31I GGTCTC 2 cut(s) 1970, 3228
Eco32I GATATC 1 cut(s) 2470
Eco47I GGWCC 4 cut(s) 243, 454, 1357, 1948
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 4 cut(s) 300, 859, 2403, 3193
Eco81I CCTNAGG 2 cut(s) 651, 2433
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 3 cut(s) 1771, 1772, 1948
EcoRI GAATTC 1 cut(s) 289
EcoRII CCWGG 5 cut(s) 862, 1245, 1359, 2712, 3435
EcoRV GATATC 1 cut(s) 2470
EcoT14I CCWWGG 4 cut(s) 2211, 2373, 2508, 3378
EcoT38I GRGCYC 2 cut(s) 105, 1775
ErhI CCWWGG 4 cut(s) 2211, 2373, 2508, 3378
Esp3I CGTCTC 1 cut(s) 82
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FaqI GGGAC 3 cut(s) 651, 1934, 2011
FauI CCCGC 4 cut(s) 150, 788, 906, 3240
FauNDI CATATG 1 cut(s) 721
FblI GTMKAC 3 cut(s) 1849, 2197, 2592
FriOI GRGCYC 2 cut(s) 105, 1775
GlaI GCGC 1 cut(s) 232
GsaI CCCAGC 1 cut(s) 1211
GsuI CTGGAG 2 cut(s) 2130, 2536
HaeIII GGCC 9 cut(s) 1773, 1844, 2033, 2055, 2210, 2481, 2655, 2882, 3429
HapII CCGG 5 cut(s) 241, 1124, 2013, 3084, 3091
HgaI GACGC 1 cut(s) 821
HhaI GCGC 1 cut(s) 233
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 2 cut(s) 1252, 3231
HindII GTYRAC 2 cut(s) 1252, 3231
HpaII CCGG 5 cut(s) 241, 1124, 2013, 3084, 3091
HphI GGTGA 3 cut(s) 928, 1996, 2878
Hpy166II GTNNAC 9 cut(s) 1252, 1366, 1850, 2004, 2198, 2284, 2593, 2682, 3231
Hpy8I GTNNAC 9 cut(s) 1252, 1366, 1850, 2004, 2198, 2284, 2593, 2682, 3231
HpyAV CCTTC 7 cut(s) 883, 942, 2048, 2213, 2309, 2822, 2854
HpyCH4IV ACGT 3 cut(s) 2000, 2047, 2077
HpySE526I ACGT 3 cut(s) 2000, 2047, 2077
HspAI GCGC 1 cut(s) 231
Kpn2I TCCGGA 2 cut(s) 240, 2012
KspI CCGCGG 2 cut(s) 171, 198
LguI GCTCTTC 1 cut(s) 2222
LweI GCATC 2 cut(s) 2430, 3011
MabI ACCWGGT 1 cut(s) 1359
MaeII ACGT 3 cut(s) 2000, 2047, 2077
MaeIII GTNAC 7 cut(s) 697, 1492, 1986, 2740, 2884, 3055, 3409
MbiI CCGCTC 2 cut(s) 601, 2946
MflI RGATCY 3 cut(s) 1572, 2808, 2951
MhlI GDGCHC 3 cut(s) 105, 1605, 1775
MlsI TGGCCA 2 cut(s) 1844, 2655
MluNI TGGCCA 2 cut(s) 1844, 2655
MlyI GAGTC 5 cut(s) 403, 1046, 1247, 1361, 2543
MmeI TCCRAC 1 cut(s) 3150
Mox20I TGGCCA 2 cut(s) 1844, 2655
MroI TCCGGA 2 cut(s) 240, 2012
MroXI GAANNNNTTC 1 cut(s) 2583
MscI TGGCCA 2 cut(s) 1844, 2655
MslI CAYNNNNRTG 3 cut(s) 18, 512, 2661
Msp20I TGGCCA 2 cut(s) 1844, 2655
MspA1I CMGCKG 3 cut(s) 170, 197, 1436
MspCI CTTAAG 1 cut(s) 2492
MspI CCGG 5 cut(s) 241, 1124, 2013, 3084, 3091
MspR9I CCNGG 6 cut(s) 864, 1247, 1361, 2714, 3091, 3437
Mva1269I GAATGC 3 cut(s) 1335, 2697, 3126
MvaI CCWGG 5 cut(s) 864, 1247, 1361, 2714, 3437
MvnI CGCG 2 cut(s) 170, 197
NciI CCSGG 1 cut(s) 3091
NdeI CATATG 1 cut(s) 721
NlaIV GGNNCC 8 cut(s) 140, 157, 539, 1641, 1773, 1950, 2953, 3088
NmeAIII GCCGAG 2 cut(s) 1905, 2308
NmuCI GTSAC 6 cut(s) 697, 1492, 1986, 2884, 3055, 3409
NspI RCATGY 2 cut(s) 2259, 2290
PaeI GCATGC 1 cut(s) 2259
PceI AGGCCT 2 cut(s) 2210, 2481
PciSI GCTCTTC 1 cut(s) 2222
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 1063
PctI GAATGC 3 cut(s) 1335, 2697, 3126
PdmI GAANNNNTTC 1 cut(s) 2583
PfoI TCCNGGA 2 cut(s) 2712, 3089
PleI GAGTC 5 cut(s) 402, 1045, 1247, 1361, 2543
PpsI GAGTC 5 cut(s) 402, 1045, 1247, 1361, 2543
PpuMI RGGWCCY 1 cut(s) 1948
PshAI GACNNNNGTC 2 cut(s) 337, 1830
PshBI ATTAAT 1 cut(s) 1131
PsiI TTATAA 1 cut(s) 2070
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 1 cut(s) 1948
Psp6I CCWGG 5 cut(s) 862, 1245, 1359, 2712, 3435
PspFI CCCAGC 1 cut(s) 1207
PspGI CCWGG 5 cut(s) 862, 1245, 1359, 2712, 3435
PspN4I GGNNCC 8 cut(s) 140, 157, 539, 1641, 1773, 1950, 2953, 3088
PspOMI GGGCCC 1 cut(s) 1771
PspPI GGNCC 7 cut(s) 243, 454, 1357, 1771, 1772, 1948, 2031
PspPPI RGGWCCY 1 cut(s) 1948
PsrI GAACNNNNNNTAC 2 cut(s) 3055, 3087
PstI CTGCAG 4 cut(s) 1441, 1564, 1610, 2600
PstNI CAGNNNCTG 2 cut(s) 428, 614
PsuI RGATCY 3 cut(s) 1572, 2808, 2951
PvuII CAGCTG 1 cut(s) 1436
RsaI GTAC 4 cut(s) 679, 1783, 2283, 3073
RsaNI GTAC 4 cut(s) 678, 1782, 2282, 3072
RseI CAYNNNNRTG 3 cut(s) 18, 512, 2661
SacI GAGCTC 1 cut(s) 105
SacII CCGCGG 2 cut(s) 171, 198
SapI GCTCTTC 1 cut(s) 2222
Sau96I GGNCC 7 cut(s) 243, 454, 1357, 1771, 1772, 1948, 2031
SchI GAGTC 5 cut(s) 403, 1046, 1247, 1361, 2543
ScrFI CCNGG 6 cut(s) 864, 1247, 1361, 2714, 3091, 3437
SduI GDGCHC 3 cut(s) 105, 1605, 1775
SexAI ACCWGGT 1 cut(s) 1359
SfaNI GCATC 2 cut(s) 2430, 3011
SfcI CTRYAG 5 cut(s) 1437, 1560, 1606, 1808, 2596
Sfr303I CCGCGG 2 cut(s) 171, 198
SgrBI CCGCGG 2 cut(s) 171, 198
SinI GGWCC 4 cut(s) 243, 454, 1357, 1948
SmiMI CAYNNNNRTG 3 cut(s) 18, 512, 2661
SmlI CTYRAG 5 cut(s) 704, 827, 1031, 1821, 2492
SmoI CTYRAG 5 cut(s) 704, 827, 1031, 1821, 2492
SpeI ACTAGT 2 cut(s) 38, 674
SphI GCATGC 1 cut(s) 2259
SseBI AGGCCT 2 cut(s) 2210, 2481
SspI AATATT 3 cut(s) 1942, 2361, 2646
SstI GAGCTC 1 cut(s) 105
StuI AGGCCT 2 cut(s) 2210, 2481
StyD4I CCNGG 6 cut(s) 862, 1245, 1359, 2712, 3089, 3435
StyI CCWWGG 4 cut(s) 2211, 2373, 2508, 3378
TaiI ACGT 3 cut(s) 2003, 2050, 2080
TaqI TCGA 3 cut(s) 1164, 1512, 2722
TaqII GACCGA 1 cut(s) 1150
TatI WGTACW 1 cut(s) 1781
TauI GCSGC 7 cut(s) 71, 111, 135, 173, 197, 1470, 2144
TseFI GTSAC 6 cut(s) 697, 1492, 1986, 2884, 3055, 3409
Tsp45I GTSAC 6 cut(s) 697, 1492, 1986, 2884, 3055, 3409
TspGWI ACGGA 2 cut(s) 2414, 2622
Vha464I CTTAAG 1 cut(s) 2492
VpaK11BI GGWCC 4 cut(s) 243, 454, 1357, 1948
VspI ATTAAT 1 cut(s) 1131
XceI RCATGY 2 cut(s) 2259, 2290
XcmI CCANNNNNNNNNTGG 1 cut(s) 629
XmiI GTMKAC 3 cut(s) 1849, 2197, 2592
XmnI GAANNNNTTC 1 cut(s) 2583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.