Rh2CG060800

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001838
Physical Location & Seq
Reverse (-)
4947237 .. 4954141
6905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG060800.1

Sequence Viewer

Length: 4026 bp
ATGAGTGATGAACACAGCCATGACATGACCACTACTACTAGTTCATCACAGATTCTATGCCACACGGCGGCTTTCGTCTCCGAAACACTTTCGCAATCGGAGCTCCGCCGCCACCTCTTCTCCACGCTCCGCCGCAGGCTCCCGCCACCGTTGGGTTCCAGCCAAACCGCGGCTATTAAATCCCTCAACCTCGCCGCGGAAACCCTAGAAAACGCCATCGCCGAATCCAGCGCAGCCGTCCGGACCTCCTCCCTCCGCCTCTGTGAGAAGCTCCTCCTCTCCGGGCCCAAAAGCCCCTTCTCCTCGCTCCTCCTCTCCCTCATCTACGGCCTCTGCCGCCGCTCGATCGACGCCGCCGTCTGCATCCTGGAGATCTTCCGATCCGAGCCGGCGCTCGCCCGGACGGAATTGGCTCCGGTGCTGTTCGAGGAGCTCTTCCTCGTCCACCTCCTCCCGGTTCTCCAGTGGTACAATCGCCGGCGATCGCAGATTCTGCCGTCGCTGTCCCCAAATGTCAGCTACGAAAACGACGACGACGACGTTTCGATCAACGACATGTCGGTGGCGTTTCCGGCGGGGTCGAATTCGCTGTCGAAGATGAGCGGGAACCAGACGGCGGGGCTGAAGGAGCTGGAGAGCCGCTACGAGAGGGTTTTGGATGAGAATTGTAGGGTTTTCAGTGAGTATTTCAGGGAGGTTTTGGAGAATAAAGATGGAGGAAGAGTGATTGATCCACCGGCTGTGGTGTTGGAATTGAGTGAAGGAAGTGATGAGTTCCGGTATGATGATCGGAGCACCGGAGAAGATGAAACCGGATTTGAAAACGGACGGTACAATCCAATTTGGGCTGAAGCAGAGAATTCAGTTGAATCGTTCAGCAGGGGCAGCAGTGTAAGAAGAAGACCCAAGTCTCCCTCATTTTATCCTCAAAGAGTTGCACTTGATAATATCTTACCCTCATGGAGTCTGGGAGCAACGCCAAATGTAAATTCAGATTCTGAGCTGGAAGAATCTTCTTTGGAGGACCATTCTCTTGATTCCTTTTTGGATTCCGAAGCTGAAATTGAGGAAAACAACACAAGAATGGAATTATTTGATTCTAGGCGGAACCAAATCCAAAAGCTAAAGCAGCCCATTTCCGCAGAATCAAGTCGTTCATCTCCAAATCCGCTCATGGCAGATTCTGAAAACCAATCTGGTGGTGGAAAATTTACACCTCCTAAGGACTTTGTGTGTCCCATAACTAGTACCCTTTTTGATGATCCAGTGACACTTGAGACTGGTCAGACATATGAGCGAAAAGCCATCCAAGAATGGCTTGATAGAGGAAACTCAACCTGCCCAATTACCCGCCAGAATTTGCAAAGCACCCAACTGCCTAAGACCAACTATGTCCTCAAGCGTCTAATTGCAAGTTGGCAAGAACAGAACCCTGGTATAGCCTTCAGCCCATCTGATAATCCAAGCCCGCAGATTGAGCCAATGGTGAAACCCATAATGCCTTCAACTTCTCCTGTCAGTGTCATAAGCCAAGCTGCTATTGATAGTGCAGTTAGTGAGTTAAGAATTGCCATTACCAATCTTTGTATGTCAGAAATTCTCAAGGAGTCTGAAATGGCTGTTCTTCGTATTGAGCGTTTCTGGCAGGAAGCAAATGTGGAATGGGATATTCAGAATATGCTCTCAAAACCTCCGGTTATTAATGGTTTCGTGGAGGTTCTTTTCAATTCGGTCGATCCTAGAGTTCTAACAGCCACAGTTTTCCTCTTGTCTGAGCTGGGTTCAAGAGACAATGCTGTGATTCAGACACTAACCCAGGTTGACTCTGATGTGGAATGTATTGTGGCTCTTTTCAAAAAGGGGTTGATGGAGGCTGTTGTCTTGATATATCTGCTAAGGCATTGCATTCTTAATCTAGTGGAGATGGACCTGGTGGACTCTCTCTTAATGGTTATTAGAAAGAGAGATGATGATTGGGCTAATATGTGTCTAAAGCCTAAATCAGCTGCAGTGGTTTTACTGGGGCATATTGTTGGCGGCAGTGAATCAATCGCCAACACTGTGACTTCTGAAAAATTACTCGAAAGAATAATCAGCAGTTTGGAATCTGAATCAGCAGAAGAGAAGACTGCAGCGATTGGGATCTTATTAAGATGTATGCAACAGGATGGGCACTGCAGGAACACAATAGCTGATAAAGCTGAGTTGGCTCCTGTTTTGGATAGCTTCACGGGTGCAGAGGATGAAGAACGGTTTGAGATAGTTCTTTTTTTGTCCGAGTTAGTGAAATTAAACAGGAGGACATTCAATGAACAAATCCTGCATATCATAAAGGATGAGGGCCCTTTCAGTACAATGCACACCCTCCTTATTTATCTACAGACTGCTGCTCAAGACCAATGTCCTGTTGTGGCCAGTCTACTACTTCAACTTGATCTTCTGGCAGAGCCGAGAAAGATGAGCATATACCGTGAAGAGGCAATGGACACTCTCATCTCATGTCTCAGAAATATTGAGGTCCCTGCTGCCCAAATCGCTGCTGCTGAGACCATTATGTCACTGCAAGGGACGTTCACCACATCCGGAAAGCCTCTTGCTAGGGCCGTCCTTCTAAAACGTGCTGGCCTTGACAAAATTTATAAACAACGTGTGCGAATGGATCAACTTAGCTACTTTTCTGGAGAAGATGAAACTGTTGAGGAAGAAAAGGCGGCTGATAACTGGGAAAGAAAAATGGCATTTGTTTTAGCAAGCCACGAGTTTGGTCTACTTTTTGAGGCCTTGGCAGAAGGATTGAAGAGCAGAATCTCAGAATTATGTTCTGCATGCTTTGTGTCAGCCACATGGCTCGTACACATGCTCAATGCCCTTCCAGATACAGGAATACGAGGAGCAGCTCGGCTGTGCTTGATTAAACGGTTCATATCAATATTCAAGTCTGCCAAGGATACTGAAGACAAAGCACTTGCTTTGCTGGCGTTGGGCAGCTTCATCCGTGATCCTGAGGGGATGCAAGAATTAAATTCTTGCAGGAGGGATATCGTAAAAGGCCTGAGAGAGCTTAAGAGATACACTCCCTTGGCATTTCAAATGCTAAAAGTCTTTTCCGATGGGCAAGACTCCAGTGCCGAATTGTGGGATCATAAAGAATTAGTTCAAGTAGACTGCAGTGAGAACGGAGAGGTTCTTTCAGTAGTTTGTTTCAAGGATAAAATATTTTCTGGCCATTCAGATGGAACTATAAAGGTGTGGACGGGTAAAGGTAGCATTCTCCATCTAATCCAGGAAATTCGAGAACATACAAAGGCAGTTACTAGCTTGGCAATTTTGCAATCAAAGGAGATTCTATACAGTGGCTCACTAGATAGAACAACAAGGATCTGGTCTATCAGTAATGAAGGAATCCAGTGTGTAGAAGTCCATGATATGAAGGATCAAGTTCATAATCTGGCCGTCACCAATAGTTTTTCATGCTTCATTCCACAAAGCAATGGTATCAAGGCTCACTCATGGAGCGGAGGATCCAAAATGTTAAATTCCAGCAAATCTGTAAAGTGCTTTGCTCTGGTAAATGGGAAAATATATTGTGGATGCCATGATAGCAGTGTTCAGGAGATTGATTTGGTCACAGGAACACTCAGTACCATTCAAACCGGCTCCCGGAAATTACTTGGGAAATCAAATCCTATCCATTGCATTCAGTATCACAGTGGAATGATATATGCAGCAGGTTCTTCGTTGGATGGAGCAGCAGTGAAGATTTGGAGTGCTGCAAACTTCAGTTTGGTTGGGTCTCTGTCAACTACACTAGAAGTGCGGGCTATGGCAATAAGCTCGGAACTGATCTATTTGGGAGGTAAAGGAGGATGTGTGGAAATTTGGGATAGGGAGAAGCAGAACAAAATAATAGACACACTACAAACTGGCACAAACTGTAAGGTTCTTTCCTTGGCTATTGATCCCAATGAGGAAGTTTTGGTCACTGGAACATCTGATGGCCGAATCCAGGCATGGGGACTAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1341

Amino Acids

148.51

Weight (kDa)

5.15

Isoelectric Point (pI)

50.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 22 - 238 1.3e-20 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 407 - 477 5.1e-17 U-box domain
ARM_LIN_2nd PF23654 517 - 674 9.6e-60 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 683 - 1036 2.5e-144 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
WD40_Prp19 PF24814 1033 - 1145 1e-08 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1038 - 1188 1.3e-07 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 1041 - 1147 1.1e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1045 - 1163 7.1e-09 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 1061 - 1155 3.7e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 1064 - 1134 8.2e-07 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 1069 - 1154 3.1e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 1070 - 1151 4.4e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 1158 - 1339 2.1e-07 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 1181 - 1339 8.4e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1192 - 1338 1.3e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1195 - 1335 7.3e-08 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2640
AasI GACNNNNNNGTC 2 cut(s) 356, 2554
Acc36I ACCTGC 2 cut(s) 1346, 3719
AccBSI CCGCTC 4 cut(s) 342, 603, 1171, 3516
AccI GTMKAC 3 cut(s) 2419, 2767, 3162
AccII CGCG 2 cut(s) 170, 197
AccIII TCCGGA 2 cut(s) 240, 2582
AcoI YGGCCR 4 cut(s) 2412, 3223, 3450, 3997
AcuI CTGAAG 5 cut(s) 644, 870, 1429, 2973, 3763
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 6 cut(s) 470, 833, 1249, 2353, 2853, 3643
AflII CTTAAG 1 cut(s) 3062
AflIII ACRYGT 2 cut(s) 555, 2647
AhlI ACTAGT 2 cut(s) 38, 1244
AjnI CCWGG 6 cut(s) 366, 1432, 1815, 1929, 3282, 4005
AjuI GAANNNNNNNTTGG 4 cut(s) 281, 313, 973, 1005
Alw21I GWGCWC 3 cut(s) 105, 435, 797
Alw26I GTCTC 7 cut(s) 82, 915, 1271, 1782, 2507, 2540, 3798
AlwNI CAGNNNCTG 3 cut(s) 493, 998, 1184
Aor13HI TCCGGA 2 cut(s) 240, 2582
ApaI GGGCCC 2 cut(s) 288, 2345
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AseI ATTAAT 1 cut(s) 1701
AsiSI GCGATCGC 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 3153
AspLEI GCGC 2 cut(s) 233, 394
AspS9I GGNCC 9 cut(s) 243, 284, 285, 1024, 1927, 2341, 2342, 2518, 2601
AsuC2I CCSGG 4 cut(s) 283, 400, 455, 3661
AsuHPI GGTGA 3 cut(s) 1498, 2566, 3448
AvaII GGWCC 4 cut(s) 243, 1024, 1927, 2518
AxyI CCTNAGG 2 cut(s) 1221, 3003
BaeGI GKGCMC 3 cut(s) 288, 2175, 2345
BalI TGGCCA 2 cut(s) 2414, 3225
BamHI GGATCC 1 cut(s) 3521
BanII GRGCYC 4 cut(s) 105, 288, 435, 2345
BauI CACGAG 1 cut(s) 2756
BbsI GAAGAC 3 cut(s) 907, 2132, 2961
Bbv12I GWGCWC 3 cut(s) 105, 435, 797
BceAI ACGGC 8 cut(s) 81, 221, 341, 343, 481, 630, 2588, 3437
BcgI CGANNNNNNTGC 4 cut(s) 3717, 3751, 3816, 3850
BciT130I CCWGG 6 cut(s) 368, 1434, 1817, 1931, 3284, 4007
BciVI GTATCC 1 cut(s) 2941
BcnI CCSGG 4 cut(s) 283, 400, 455, 3661
BcoDI GTCTC 7 cut(s) 82, 915, 1271, 1782, 2507, 2540, 3798
BcuI ACTAGT 2 cut(s) 38, 1244
BfmI CTRYAG 5 cut(s) 2007, 2130, 2176, 2378, 3166
BfoI RGCGCY 1 cut(s) 395
BfrI CTTAAG 1 cut(s) 3062
BfuAI ACCTGC 2 cut(s) 1346, 3719
BfuI GTATCC 1 cut(s) 2941
BglII AGATCT 1 cut(s) 372
Bme18I GGWCC 4 cut(s) 243, 1024, 1927, 2518
BmgT120I GGNCC 9 cut(s) 243, 284, 285, 1024, 1927, 2341, 2342, 2518, 2601
BmrI ACTGGG 2 cut(s) 2030, 2731
BmsI GCATC 3 cut(s) 372, 3000, 3581
BmuI ACTGGG 2 cut(s) 2030, 2731
BoxI GACNNNNGTC 2 cut(s) 907, 2400
BpiI GAAGAC 3 cut(s) 907, 2132, 2961
BplI GAGNNNNNCTC 2 cut(s) 3058, 3090
BpmI CTGGAG 5 cut(s) 389, 446, 653, 2700, 3106
Bpu10I CCTNAGC 1 cut(s) 1895
BpuEI CTTGAG 4 cut(s) 1295, 1382, 1586, 2376
BpuMI CCSGG 4 cut(s) 283, 400, 455, 3661
BsaBI GATNNNNATC 1 cut(s) 2141
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 2 cut(s) 2540, 3798
BsaJI CCNNGG 8 cut(s) 168, 195, 1432, 1815, 2781, 2943, 3078, 3948
BsaWI WCCGGW 7 cut(s) 240, 415, 777, 797, 812, 1693, 2582
BsaXI ACNNNNNCTCC 8 cut(s) 104, 134, 708, 738, 3738, 3768, 3855, 3885
Bse118I RCCGGY 4 cut(s) 388, 477, 736, 3653
Bse21I CCTNAGG 2 cut(s) 1221, 3003
Bse3DI GCAATG 4 cut(s) 1900, 2487, 3496, 3691
Bse8I GATNNNNATC 1 cut(s) 2141
BseAI TCCGGA 2 cut(s) 240, 2582
BseBI CCWGG 6 cut(s) 368, 1434, 1817, 1931, 3284, 4007
BseDI CCNNGG 8 cut(s) 168, 195, 1432, 1815, 2781, 2943, 3078, 3948
BseJI GATNNNNATC 1 cut(s) 2141
BseMI GCAATG 4 cut(s) 1900, 2487, 3496, 3691
BseMII CTCAG 9 cut(s) 990, 1764, 2193, 2518, 2535, 2823, 2994, 3044, 3652
BseRI GAGGAG 9 cut(s) 238, 263, 266, 292, 299, 302, 440, 443, 2904
BseSI GKGCMC 3 cut(s) 288, 2175, 2345
BseYI CCCAGC 1 cut(s) 1777
BsgI GTGCAG 2 cut(s) 1569, 2256
Bsh1236I CGCG 2 cut(s) 170, 197
Bsh1285I CGRYCG 3 cut(s) 348, 485, 1734
BsiEI CGRYCG 3 cut(s) 348, 485, 1734
BsiHKAI GWGCWC 3 cut(s) 105, 435, 797
BslFI GGGAC 4 cut(s) 490, 1221, 2504, 2581
BsmAI GTCTC 7 cut(s) 82, 915, 1271, 1782, 2507, 2540, 3798
BsmBI CGTCTC 1 cut(s) 82
BsmFI GGGAC 4 cut(s) 490, 1221, 2504, 2581
BsmI GAATGC 3 cut(s) 1905, 3267, 3696
Bso31I GGTCTC 2 cut(s) 2540, 3798
Bsp120I GGGCCC 2 cut(s) 284, 2341
Bsp1286I GDGCHC 6 cut(s) 105, 288, 435, 797, 2175, 2345
Bsp13I TCCGGA 2 cut(s) 240, 2582
BspCNI CTCAG 9 cut(s) 991, 1765, 2194, 2517, 2536, 2822, 2995, 3045, 3651
BspEI TCCGGA 2 cut(s) 240, 2582
BspFNI CGCG 2 cut(s) 170, 197
BspMAI CTGCAG 4 cut(s) 2011, 2134, 2180, 3170
BspMI ACCTGC 2 cut(s) 1346, 3719
BspQI GCTCTTC 2 cut(s) 440, 2792
BspTI CTTAAG 1 cut(s) 3062
BspTNI GGTCTC 2 cut(s) 2540, 3798
BsrBI CCGCTC 4 cut(s) 342, 603, 1171, 3516
BsrDI GCAATG 4 cut(s) 1900, 2487, 3496, 3691
BsrFI RCCGGY 4 cut(s) 388, 477, 736, 3653
BssAI RCCGGY 4 cut(s) 388, 477, 736, 3653
BssECI CCNNGG 8 cut(s) 168, 195, 1432, 1815, 2781, 2943, 3078, 3948
BssNI GRCGYC 1 cut(s) 351
BssSI CACGAG 1 cut(s) 2756
BssT1I CCWWGG 4 cut(s) 2781, 2943, 3078, 3948
Bst2BI CACGAG 1 cut(s) 2756
Bst2UI CCWGG 6 cut(s) 368, 1434, 1817, 1931, 3284, 4007
Bst6I CTCTTC 6 cut(s) 122, 440, 715, 2115, 2469, 2792
BstACI GRCGYC 1 cut(s) 351
BstAFI CTTAAG 1 cut(s) 3062
BstAPI GCANNNNNTGC 1 cut(s) 493
BstDSI CCRYGG 2 cut(s) 168, 195
BstFNI CGCG 2 cut(s) 170, 197
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 2 cut(s) 233, 394
BstMAI GTCTC 7 cut(s) 82, 915, 1271, 1782, 2507, 2540, 3798
BstMCI CGRYCG 3 cut(s) 348, 485, 1734
BstNI CCWGG 6 cut(s) 368, 1434, 1817, 1931, 3284, 4007
BstNSI RCATGY 3 cut(s) 559, 2829, 2860
BstPAI GACNNNNGTC 2 cut(s) 907, 2400
BstSFI CTRYAG 5 cut(s) 2007, 2130, 2176, 2378, 3166
BstSLI GKGCMC 3 cut(s) 288, 2175, 2345
BstUI CGCG 2 cut(s) 170, 197
BstV2I GAAGAC 3 cut(s) 907, 2132, 2961
BstX2I RGATCY 4 cut(s) 372, 2142, 3378, 3521
BstXI CCANNNNNNTGG 3 cut(s) 1199, 2762, 3233
BstYI RGATCY 4 cut(s) 372, 2142, 3378, 3521
Bsu36I CCTNAGG 2 cut(s) 1221, 3003
BsuI GTATCC 1 cut(s) 2941
BtgI CCRYGG 2 cut(s) 168, 195
BtgZI GCGATG 1 cut(s) 202
BtsI GCAGTG 8 cut(s) 895, 2016, 2047, 2173, 2558, 3175, 3610, 3759
BveI ACCTGC 2 cut(s) 1346, 3719
CaiI CAGNNNCTG 3 cut(s) 493, 998, 1184
CfoI GCGC 2 cut(s) 233, 394
Cfr10I RCCGGY 4 cut(s) 388, 477, 736, 3653
Cfr13I GGNCC 9 cut(s) 243, 284, 285, 1024, 1927, 2341, 2342, 2518, 2601
Cfr42I CCGCGG 2 cut(s) 171, 198
CseI GACGC 2 cut(s) 359, 1391
CsiI ACCWGGT 1 cut(s) 1929
Csp6I GTAC 6 cut(s) 469, 832, 1248, 2352, 2852, 3642
CviQI GTAC 6 cut(s) 469, 832, 1248, 2352, 2852, 3642
DrdI GACNNNNNNGTC 2 cut(s) 356, 2554
DseDI GACNNNNNNGTC 2 cut(s) 356, 2554
EaeI YGGCCR 4 cut(s) 2412, 3223, 3450, 3997
Eam1104I CTCTTC 6 cut(s) 122, 440, 715, 2115, 2469, 2792
EarI CTCTTC 6 cut(s) 122, 440, 715, 2115, 2469, 2792
EciI GGCGGA 4 cut(s) 95, 119, 245, 1120
Ecl136II GAGCTC 2 cut(s) 103, 433
Eco130I CCWWGG 4 cut(s) 2781, 2943, 3078, 3948
Eco147I AGGCCT 2 cut(s) 2780, 3051
Eco24I GRGCYC 4 cut(s) 105, 288, 435, 2345
Eco31I GGTCTC 2 cut(s) 2540, 3798
Eco32I GATATC 1 cut(s) 3040
Eco47I GGWCC 4 cut(s) 243, 1024, 1927, 2518
Eco53kI GAGCTC 2 cut(s) 103, 433
Eco57I CTGAAG 5 cut(s) 644, 870, 1429, 2973, 3763
Eco81I CCTNAGG 2 cut(s) 1221, 3003
EcoICRI GAGCTC 2 cut(s) 103, 433
EcoO109I RGGNCCY 3 cut(s) 2341, 2342, 2518
EcoRI GAATTC 2 cut(s) 583, 859
EcoRII CCWGG 6 cut(s) 366, 1432, 1815, 1929, 3282, 4005
EcoRV GATATC 1 cut(s) 3040
EcoT14I CCWWGG 4 cut(s) 2781, 2943, 3078, 3948
EcoT38I GRGCYC 4 cut(s) 105, 288, 435, 2345
ErhI CCWWGG 4 cut(s) 2781, 2943, 3078, 3948
Esp3I CGTCTC 1 cut(s) 82
FalI AAGNNNNNCTT 2 cut(s) 1302, 1334
FaqI GGGAC 4 cut(s) 490, 1221, 2504, 2581
FauI CCCGC 7 cut(s) 150, 568, 596, 610, 1358, 1476, 3810
FauNDI CATATG 1 cut(s) 1291
FblI GTMKAC 3 cut(s) 2419, 2767, 3162
FriOI GRGCYC 4 cut(s) 105, 288, 435, 2345
GlaI GCGC 2 cut(s) 232, 393
GsaI CCCAGC 1 cut(s) 1781
GsuI CTGGAG 5 cut(s) 389, 446, 653, 2700, 3106
HaeII RGCGCY 1 cut(s) 395
HgaI GACGC 2 cut(s) 359, 1391
HhaI GCGC 2 cut(s) 233, 394
Hin1I GRCGYC 1 cut(s) 351
Hin6I GCGC 2 cut(s) 231, 392
HinP1I GCGC 2 cut(s) 231, 392
HincII GTYRAC 2 cut(s) 1822, 3801
HindII GTYRAC 2 cut(s) 1822, 3801
HphI GGTGA 3 cut(s) 1498, 2566, 3448
Hpy99I CGWCG 6 cut(s) 353, 502, 533, 536, 539, 542
HpyCH4IV ACGT 4 cut(s) 540, 2570, 2617, 2647
HpySE526I ACGT 4 cut(s) 540, 2570, 2617, 2647
Hsp92I GRCGYC 1 cut(s) 351
HspAI GCGC 2 cut(s) 231, 392
Kpn2I TCCGGA 2 cut(s) 240, 2582
KroI GCCGGC 2 cut(s) 388, 477
KroNI GCCGGC 2 cut(s) 390, 479
KspI CCGCGG 2 cut(s) 171, 198
LguI GCTCTTC 2 cut(s) 440, 2792
LweI GCATC 3 cut(s) 372, 3000, 3581
MabI ACCWGGT 1 cut(s) 1929
MaeII ACGT 4 cut(s) 540, 2570, 2617, 2647
MaeIII GTNAC 7 cut(s) 1267, 2062, 2556, 3310, 3454, 3625, 3979
MbiI CCGCTC 4 cut(s) 342, 603, 1171, 3516
MflI RGATCY 4 cut(s) 372, 2142, 3378, 3521
MhlI GDGCHC 6 cut(s) 105, 288, 435, 797, 2175, 2345
MlsI TGGCCA 2 cut(s) 2414, 3225
MluNI TGGCCA 2 cut(s) 2414, 3225
MlyI GAGTC 5 cut(s) 973, 1616, 1817, 1931, 3113
MmeI TCCRAC 2 cut(s) 729, 3720
Mox20I TGGCCA 2 cut(s) 2414, 3225
MreI CGCCGGCG 1 cut(s) 477
MroI TCCGGA 2 cut(s) 240, 2582
MroNI GCCGGC 2 cut(s) 388, 477
MroXI GAANNNNTTC 1 cut(s) 3153
MscI TGGCCA 2 cut(s) 2414, 3225
MslI CAYNNNNRTG 4 cut(s) 18, 560, 1082, 3231
Msp20I TGGCCA 2 cut(s) 2414, 3225
MspA1I CMGCKG 3 cut(s) 170, 197, 2006
MspCI CTTAAG 1 cut(s) 3062
Mva1269I GAATGC 3 cut(s) 1905, 3267, 3696
MvaI CCWGG 6 cut(s) 368, 1434, 1817, 1931, 3284, 4007
MvnI CGCG 2 cut(s) 170, 197
NaeI GCCGGC 2 cut(s) 390, 479
NciI CCSGG 4 cut(s) 283, 400, 455, 3661
NdeI CATATG 1 cut(s) 1291
NgoMIV GCCGGC 2 cut(s) 388, 477
NmeAIII GCCGAG 2 cut(s) 2475, 2878
NmuCI GTSAC 6 cut(s) 1267, 2062, 2556, 3454, 3625, 3979
NspI RCATGY 3 cut(s) 559, 2829, 2860
PaeI GCATGC 1 cut(s) 2829
PceI AGGCCT 2 cut(s) 2780, 3051
PciI ACATGT 1 cut(s) 555
PciSI GCTCTTC 2 cut(s) 440, 2792
PcsI WCGNNNNNNNCGW 6 cut(s) 219, 354, 528, 534, 537, 1633
PctI GAATGC 3 cut(s) 1905, 3267, 3696
PdiI GCCGGC 2 cut(s) 390, 479
PdmI GAANNNNTTC 1 cut(s) 3153
PfoI TCCNGGA 3 cut(s) 366, 3282, 3659
Ple19I CGATCG 2 cut(s) 348, 485
PleI GAGTC 5 cut(s) 972, 1615, 1817, 1931, 3113
PpsI GAGTC 5 cut(s) 972, 1615, 1817, 1931, 3113
PpuMI RGGWCCY 1 cut(s) 2518
PscI ACATGT 1 cut(s) 555
PshAI GACNNNNGTC 2 cut(s) 907, 2400
PshBI ATTAAT 1 cut(s) 1701
PsiI TTATAA 1 cut(s) 2640
Psp124BI GAGCTC 2 cut(s) 105, 435
Psp5II RGGWCCY 1 cut(s) 2518
Psp6I CCWGG 6 cut(s) 366, 1432, 1815, 1929, 3282, 4005
PspFI CCCAGC 1 cut(s) 1777
PspGI CCWGG 6 cut(s) 366, 1432, 1815, 1929, 3282, 4005
PspOMI GGGCCC 2 cut(s) 284, 2341
PspPI GGNCC 9 cut(s) 243, 284, 285, 1024, 1927, 2341, 2342, 2518, 2601
PspPPI RGGWCCY 1 cut(s) 2518
PsrI GAACNNNNNNTAC 2 cut(s) 3625, 3657
PstI CTGCAG 4 cut(s) 2011, 2134, 2180, 3170
PstNI CAGNNNCTG 3 cut(s) 493, 998, 1184
PsuI RGATCY 4 cut(s) 372, 2142, 3378, 3521
PvuI CGATCG 2 cut(s) 348, 485
PvuII CAGCTG 1 cut(s) 2006
RgaI GCGATCGC 1 cut(s) 485
RsaI GTAC 6 cut(s) 470, 833, 1249, 2353, 2853, 3643
RsaNI GTAC 6 cut(s) 469, 832, 1248, 2352, 2852, 3642
RseI CAYNNNNRTG 4 cut(s) 18, 560, 1082, 3231
SacI GAGCTC 2 cut(s) 105, 435
SacII CCGCGG 2 cut(s) 171, 198
SapI GCTCTTC 2 cut(s) 440, 2792
Sau96I GGNCC 9 cut(s) 243, 284, 285, 1024, 1927, 2341, 2342, 2518, 2601
SchI GAGTC 5 cut(s) 973, 1616, 1817, 1931, 3113
SduI GDGCHC 6 cut(s) 105, 288, 435, 797, 2175, 2345
SexAI ACCWGGT 1 cut(s) 1929
SfaAI GCGATCGC 1 cut(s) 485
SfaNI GCATC 3 cut(s) 372, 3000, 3581
SfcI CTRYAG 5 cut(s) 2007, 2130, 2176, 2378, 3166
Sfr303I CCGCGG 2 cut(s) 171, 198
SgfI GCGATCGC 1 cut(s) 485
SgrAI CRCCGGYG 1 cut(s) 477
SgrBI CCGCGG 2 cut(s) 171, 198
SinI GGWCC 4 cut(s) 243, 1024, 1927, 2518
SmiMI CAYNNNNRTG 4 cut(s) 18, 560, 1082, 3231
SmlI CTYRAG 5 cut(s) 1274, 1397, 1601, 2391, 3062
SmoI CTYRAG 5 cut(s) 1274, 1397, 1601, 2391, 3062
SpeI ACTAGT 2 cut(s) 38, 1244
SphI GCATGC 1 cut(s) 2829
SseBI AGGCCT 2 cut(s) 2780, 3051
SspI AATATT 3 cut(s) 2512, 2931, 3216
SstI GAGCTC 2 cut(s) 105, 435
StuI AGGCCT 2 cut(s) 2780, 3051
StyI CCWWGG 4 cut(s) 2781, 2943, 3078, 3948
TaiI ACGT 4 cut(s) 543, 2573, 2620, 2650
TaqI TCGA 9 cut(s) 344, 348, 426, 545, 581, 593, 1734, 2082, 3292
TaqII GACCGA 1 cut(s) 1720
TatI WGTACW 1 cut(s) 2351
TseFI GTSAC 6 cut(s) 1267, 2062, 2556, 3454, 3625, 3979
Tsp45I GTSAC 6 cut(s) 1267, 2062, 2556, 3454, 3625, 3979
TspGWI ACGGA 4 cut(s) 419, 840, 2984, 3192
Vha464I CTTAAG 1 cut(s) 3062
VpaK11BI GGWCC 4 cut(s) 243, 1024, 1927, 2518
VspI ATTAAT 1 cut(s) 1701
XceI RCATGY 3 cut(s) 559, 2829, 2860
XcmI CCANNNNNNNNNTGG 1 cut(s) 1199
XmiI GTMKAC 3 cut(s) 2419, 2767, 3162
XmnI GAANNNNTTC 1 cut(s) 3153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.