Rw2G004960

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001838
Physical Location & Seq
Forward (+)
4669262 .. 4675791
6530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G004960.1

Sequence Viewer

Length: 4032 bp
ATGAGTGATGAACACAGCCATGACATGACCACTAGTACTAGTTCATCACAGATTCTATGCCACACGGCGGCTTTCGTCTCCGAAACACTTTCGCAATCGGAGCTCCGCCGCCACCGCTTCTCCACGCTCCGCCGCAGGCTCCCTCCACCGTTGGGTTCCAGCCAAACCGCGGCTATTAAATCCCTCAACCTCGCCGCGGAAACCCTAGAAAACGCCATCGCCGAATCCAGCGCAGCCGTCCGGACCTCCTCCCTCCGCCTCTGTGAGAAGCTCCTCCTCTCCGGGCCCAAAAGCCCCTTCTCCTCGCTCCTCCTCTCCCTCATCTACGGCCTCTGCCGCCGCTCGATCGACGCCGCCGTCTGCATCCTCGAGATCTTCCGGTCCGAGCCGGCGCTCGCCCGGACGGAATTGGCTCCGGTGCTGTTCGGGGAGCTCTTCCTCGTCCACCTCCTCCCGGTTCTCCAGTGGTACAATCGCCGGCGATCGCAGATTCTGCCGTCTCTGTCCCCAAATGTCAGCTACGAAAACGACGACGACGACGACGTTTCGATCAACGACATGTCGGTGGCGTTTCCGGCGGGGTCGAATTCGCTGTCGAAGATGAGCGGGAACCAGACGGCGGGGCTGAAGGAGCTGGAGAGCCGCTACGAGAGGGTTTTGGATGAGAATTGTAGGGTTTTCAGTGAGTATTTCAGGGAGGTTCTGGAGAATAAAGATGGAGGAAGAGTGATTGATCCACCGGCTGTGGTGTTGGAATTGAGTGAAGGAAGTGATGAGTTCCGGTATGATGATCGGAGCACCGGAGAAGATGAAGCCGGATTTGAAAACGGACGGTATAATCCAATTTGGGCTGAAGCAGAGAATTCAGTTGAATCGTTCAGCAGGGGCAGCAGCGTAAGAAGAAGAAGACCCAAGTCTCCCTCATTTTATCCTCAAAGAGTTGCACTTGATAATATCTTACCCTCATGGAGTCTGGGAGCAACGCCAAATGTAAATTCAGATTCTGAGCTGGAAGAATCTTCTTTGGAGGGCCATTCTCTTGATTCCTTTTTGGATTCCGAAGCTGAAATTGAGGAAAACAACACAAGAATGGAATTATTTGATTCTAGGCGGAACCAAATCCAAAAGCTAAAGCAACCCATTTCCGCAGAATCAAGTCGTTCATCTCCAAATCCGCTCATGGCAGATTCTGAAAACCAATCTGGCGGTGGAAAATTTACACCTCCTAAGGACTTTGTGTGTCCCATAACTAGTACCCTTTTTGATGATCCAGTGACACTTGAGACTGGTCAGACATATGAGCGAAAAGCCATCCAAGAATGGCTTGATAGAGGAAACTCAACCTGCCCAATTACCCGCCAGAATTTGCAAAGCACCCAACTGCCTAAGACCAACTATGTCCTCAAGCGTCTAATTGCAAGTTGGCAAGAACAGAACCCTGGTGTAGCCTTCAGCCCATCTGATAATCCAAGCCCGCAGATTGAGCCAATGGTGAAACCCATAATGCCTTCAACTTCTCCTGTCAGTGTCATAAGCCAAGCTGCTATTGATAGTGCAGTTAGTGAGTTAAGAATTGCCATTACCAATCTTTGTATGTCAGAAATTCTCAAGGAGTCTGAAATGGCTGTTCTTCGTATTGAGCGTTTCTGGCAGGAAGCAAATGTGGAATGGGATATTCAGAATATGCTCTCAAAACCTCCGGTTATTAATGGTTTCGTGGAGGTTCTTTTCAATTCGGTCGATCCTAGAGTTCTAACAGCTACAGTTTTCCTCTTGTCTGAGCTGGGTTCAAGAGACAATGCTGTGATTCAGACACTAACCCAGGTTGACTCTGATGTGGAATGTATTGTGGCTCTTTTCAAAAAGGGGTTGATGGAGGCTGTTGTCTTGATATATCTGCTAAGGCATTGCATTCTTAATCTAGTAGAGATGGACCTGGTGGACTCTCTCTTAATGGTTATTAGAAAGAGAGATGATGATTTGGTTAATATGTGTCTAAAGCCTAAATCAGCTGCAGTGGTTTTACTGGGGCATATTGTTGGTGGCAGTGAATCAATCGCCAACACCGTGACTTCTGAAAAAGCACTTGAAAGAATAATCAGCAGTTTGGAATCTGAATCAGCGGAAGAGAAGGCTGCAGCGATTGGGATCTTATTAAGATGTATGCAACAGGATGGGCACTGCAGGAACACAATAGCTGATAAAGCTGAGTTGGCTCCTGTTTTGGATAGCTTCACGGGTGCAAAGGATGAAGAACGGTTTGAGATAGTTCTCTTTTTGTCCGAGTTAGTGAAATTAAACAGGAGGACATTCAATGAACAAATCCTGCATATCATAAAGGATGAGGGCCCTTTCAGTACAATGCATACCCTCCTTATTTATCTACAGACTGCTGCTCAAGACCATTGTCCTGTTGTGGCCAGTCTACTACTTCAACTTGATCTTCTGGCAGAGCCGAGAAAGATGAGCATATACCGTGAAGAGGCAATGGACACTCTCATTTCATGTCTCAGAAATATTGAGGCCCCTGCTGCTCAAATCGCTGCTGCTGAGACCATTATGTCACTGCAAGGGACGTTCACCACATCCGGAAAGCCTCTTGCTAGGGCTGTCCTTCTAAAACGTGCTGGCCTTGACAAAATTTATAAACAACGTGTGCGAATGGATCAACTTAGCAACTTTTCTGGAGAAGTTGAAACTGTTGAGGAAGAAAAGGCAGCTGATAACTGGGAAAGAAAAATGGCATTTGTTTTAGCAAGCCACGAGTTTGGTCTACTTTTTGAGGCCTTGGCAGAAGGATTGAAGAGCAGAATCTCGGAATTATGTTCTGCATGCTTTGTGTCAGCCACATGGCTCGTACACATGCTCAATGCCCTTCCAGATACAGGAATACGAGGAGCAGCTCGGCTGTGCTTGATTAAACGGTTCATATCAATATTCAAGTCTGCCAAGGATACTGAAGACAAAGCACTTGCTTTGCTGGCGTTGGGCAGCTTCATCCGTGATCCTGAGGGGATGCAAGAATTAAATTCTTGCAGGAGGGATATCGTAAAAGGCCTGAGAGAGCTTAAGAGATACACTCCCTTGGCATTTCAAATGCTAAAAGTCTTTTCCGATGGGCAAGACTCTAGTGCCGAATTGTGGGATCATAAAGAATTAGTTCAAGTAGACTGCAGTGAGAACGGAGAGGTTCTCTCAGTAGTTTGTTTCACGGATAAAATATTTTCTGGCCATTCAGATGGAACTATAAAGGTGTGGACGGGTAAAGGTAGCATTCTCCATCTAATCCAGGAAATTCGAGAACATACAAAGGCAGTTACTAGCTTGGCAATTTTGCAATCAAAGGAGATTCTATACAGTGGCTCACTAGATAGAACAACAAGGGTCTGGTCTATCAGCAATGAAGGAATCCAGTGTGTAGAAGTCCATGATATGAAGGATCAAGTTCATAATCTGGCCGTCACCAATAGTTTTTCATGCTTCATTCCACAAAGCAATGGTATAAAGGCTCACTCATGGAGCGGAGGATCCAAAATGTTAAATTCCAGCAAATCTGTAAAGTGCTTTGCTCTGGTAAATGGGAAAATATATTGTGGATGCCATGATAGCAGTGTTCAGGAGATTGATTTGGTCACAGGAACACTCAGTACCATTCAAACCGGCTCCCGGAAATTACTTGGGAAATCAAATCCTATCCATTGCATTCAGTATCACAGTGGAATGATATATGCAGCAGGTTCTTCGTTGGATGGAGCAGCAGTGAAGATTTGGAGTGCTGCAAACTTCAGTTTGGTTGGGTCTCTGTCAACTACACTAGAAGTGCGGGCTATGGCAATAAGCTCGGAACTGATCTATTTGGGAGGTAAAGGAGGATGTGTGGAAATTTGGGATAGGGAGAAGCAGAACAAAATAATAGACACACTACAAACTGGCACAAACTGTAAGGTTCTTTCCTTGGCTATTGATCCCAATGAGGAAGTTTTGGTCACTGGAACATCTGATGGCCGAATCCAGGCATGGGGACTAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box WD40

Protein Analysis

1343

Amino Acids

148.39

Weight (kDa)

5.27

Isoelectric Point (pI)

50.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 23 - 239 1.4e-19 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 409 - 479 5.1e-17 U-box domain
ARM_LIN_2nd PF23654 519 - 676 2.9e-60 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 685 - 1038 8.2e-144 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
WD40_Prp19 PF24814 1035 - 1147 1.9e-08 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1038 - 1143 1.9e-07 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 1043 - 1149 1.8e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1047 - 1165 9.4e-09 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 1063 - 1157 2.3e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 1067 - 1136 2.5e-07 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 1072 - 1153 7.9e-08 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 1072 - 1156 5.5e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 1164 - 1341 2e-07 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 1183 - 1341 8.8e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1194 - 1340 1.3e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1198 - 1337 8e-08 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2646
AasI GACNNNNNNGTC 2 cut(s) 356, 2560
Acc36I ACCTGC 2 cut(s) 1352, 3725
AccBSI CCGCTC 4 cut(s) 342, 606, 1177, 3522
AccI GTMKAC 3 cut(s) 2425, 2773, 3168
AccII CGCG 2 cut(s) 170, 197
AccIII TCCGGA 2 cut(s) 240, 2588
AcoI YGGCCR 4 cut(s) 2418, 3229, 3456, 4003
AcuI CTGAAG 5 cut(s) 647, 873, 1435, 2979, 3769
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 6 cut(s) 37, 470, 1255, 2359, 2859, 3649
AflII CTTAAG 1 cut(s) 3068
AflIII ACRYGT 2 cut(s) 558, 2653
AhlI ACTAGT 3 cut(s) 32, 38, 1250
AjnI CCWGG 5 cut(s) 1438, 1821, 1935, 3288, 4011
AjuI GAANNNNNNNTTGG 4 cut(s) 281, 313, 979, 1011
Alw21I GWGCWC 3 cut(s) 105, 435, 800
Alw26I GTCTC 8 cut(s) 82, 504, 921, 1277, 1788, 2513, 2546, 3804
AlwNI CAGNNNCTG 3 cut(s) 493, 1004, 1190
Ama87I CYCGRG 1 cut(s) 368
Aor13HI TCCGGA 2 cut(s) 240, 2588
ApaI GGGCCC 2 cut(s) 288, 2351
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
AseI ATTAAT 1 cut(s) 1707
AsiSI GCGATCGC 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 3159
AspLEI GCGC 2 cut(s) 233, 394
AspS9I GGNCC 9 cut(s) 243, 284, 285, 381, 1030, 1933, 2347, 2348, 2524
AsuC2I CCSGG 4 cut(s) 283, 400, 455, 3667
AsuHPI GGTGA 3 cut(s) 1504, 2572, 3454
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 3 cut(s) 243, 381, 1933
AxyI CCTNAGG 2 cut(s) 1227, 3009
BaeGI GKGCMC 3 cut(s) 288, 2181, 2351
BalI TGGCCA 2 cut(s) 2420, 3231
BamHI GGATCC 1 cut(s) 3527
BanII GRGCYC 4 cut(s) 105, 288, 435, 2351
BauI CACGAG 1 cut(s) 2762
BbsI GAAGAC 2 cut(s) 913, 2967
Bbv12I GWGCWC 3 cut(s) 105, 435, 800
BceAI ACGGC 7 cut(s) 81, 221, 341, 343, 481, 633, 3443
BcgI CGANNNNNNTGC 4 cut(s) 3723, 3757, 3822, 3856
BciT130I CCWGG 5 cut(s) 1440, 1823, 1937, 3290, 4013
BciVI GTATCC 1 cut(s) 2947
BcnI CCSGG 4 cut(s) 283, 400, 455, 3667
BcoDI GTCTC 8 cut(s) 82, 504, 921, 1277, 1788, 2513, 2546, 3804
BcuI ACTAGT 3 cut(s) 32, 38, 1250
BfmI CTRYAG 6 cut(s) 1761, 2013, 2136, 2182, 2384, 3172
BfoI RGCGCY 1 cut(s) 395
BfrI CTTAAG 1 cut(s) 3068
BfuAI ACCTGC 2 cut(s) 1352, 3725
BfuI GTATCC 1 cut(s) 2947
BglII AGATCT 1 cut(s) 372
BmcAI AGTACT 1 cut(s) 37
Bme1390I CCNGG 9 cut(s) 283, 400, 455, 1440, 1823, 1937, 3290, 3667, 4013
Bme18I GGWCC 3 cut(s) 243, 381, 1933
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 9 cut(s) 243, 284, 285, 381, 1030, 1933, 2347, 2348, 2524
BmrFI CCNGG 9 cut(s) 283, 400, 455, 1440, 1823, 1937, 3290, 3667, 4013
BmrI ACTGGG 2 cut(s) 2036, 2737
BmsI GCATC 3 cut(s) 372, 3006, 3587
BmuI ACTGGG 2 cut(s) 2036, 2737
BoxI GACNNNNGTC 2 cut(s) 913, 2406
BpiI GAAGAC 2 cut(s) 913, 2967
BplI GAGNNNNNCTC 6 cut(s) 3064, 3096, 3177, 3209, 3179, 3211
BpmI CTGGAG 4 cut(s) 446, 656, 725, 2706
Bpu10I CCTNAGC 1 cut(s) 1901
BpuEI CTTGAG 4 cut(s) 1301, 1388, 1592, 2382
BpuMI CCSGG 4 cut(s) 283, 400, 455, 3667
BsaBI GATNNNNATC 1 cut(s) 2147
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 2 cut(s) 2546, 3804
BsaJI CCNNGG 8 cut(s) 168, 195, 1438, 1821, 2787, 2949, 3084, 3954
BsaWI WCCGGW 7 cut(s) 240, 378, 415, 780, 800, 1699, 2588
BsaXI ACNNNNNCTCC 8 cut(s) 104, 134, 711, 741, 3744, 3774, 3861, 3891
Bse118I RCCGGY 4 cut(s) 388, 477, 739, 3659
Bse1I ACTGG 9 cut(s) 463, 1271, 1291, 2031, 2421, 2732, 3412, 3934, 3994
Bse21I CCTNAGG 2 cut(s) 1227, 3009
Bse3DI GCAATG 5 cut(s) 1906, 2493, 3406, 3502, 3697
Bse8I GATNNNNATC 1 cut(s) 2147
BseAI TCCGGA 2 cut(s) 240, 2588
BseBI CCWGG 5 cut(s) 1440, 1823, 1937, 3290, 4013
BseDI CCNNGG 8 cut(s) 168, 195, 1438, 1821, 2787, 2949, 3084, 3954
BseJI GATNNNNATC 1 cut(s) 2147
BseMI GCAATG 5 cut(s) 1906, 2493, 3406, 3502, 3697
BseMII CTCAG 9 cut(s) 996, 1770, 2199, 2524, 2541, 3000, 3050, 3210, 3658
BseNI ACTGG 9 cut(s) 463, 1271, 1291, 2031, 2421, 2732, 3412, 3934, 3994
BseRI GAGGAG 8 cut(s) 238, 263, 266, 292, 299, 302, 440, 2910
BseSI GKGCMC 3 cut(s) 288, 2181, 2351
BseYI CCCAGC 1 cut(s) 1783
BsgI GTGCAG 1 cut(s) 1575
Bsh1236I CGCG 2 cut(s) 170, 197
Bsh1285I CGRYCG 3 cut(s) 348, 485, 1740
BsiEI CGRYCG 3 cut(s) 348, 485, 1740
BsiHKAI GWGCWC 3 cut(s) 105, 435, 800
BsiHKCI CYCGRG 1 cut(s) 368
BslFI GGGAC 3 cut(s) 490, 1227, 2587
BsmAI GTCTC 8 cut(s) 82, 504, 921, 1277, 1788, 2513, 2546, 3804
BsmBI CGTCTC 2 cut(s) 82, 504
BsmFI GGGAC 3 cut(s) 490, 1227, 2587
BsmI GAATGC 3 cut(s) 1911, 3273, 3702
Bso31I GGTCTC 2 cut(s) 2546, 3804
BsoBI CYCGRG 1 cut(s) 368
Bsp120I GGGCCC 2 cut(s) 284, 2347
Bsp1286I GDGCHC 6 cut(s) 105, 288, 435, 800, 2181, 2351
Bsp13I TCCGGA 2 cut(s) 240, 2588
BspCNI CTCAG 9 cut(s) 997, 1771, 2200, 2523, 2542, 3001, 3051, 3209, 3657
BspEI TCCGGA 2 cut(s) 240, 2588
BspFNI CGCG 2 cut(s) 170, 197
BspMAI CTGCAG 4 cut(s) 2017, 2140, 2186, 3176
BspMI ACCTGC 2 cut(s) 1352, 3725
BspQI GCTCTTC 2 cut(s) 440, 2798
BspTI CTTAAG 1 cut(s) 3068
BspTNI GGTCTC 2 cut(s) 2546, 3804
BsrBI CCGCTC 4 cut(s) 342, 606, 1177, 3522
BsrDI GCAATG 5 cut(s) 1906, 2493, 3406, 3502, 3697
BsrFI RCCGGY 4 cut(s) 388, 477, 739, 3659
BsrI ACTGG 9 cut(s) 463, 1271, 1291, 2031, 2421, 2732, 3412, 3934, 3994
BssAI RCCGGY 4 cut(s) 388, 477, 739, 3659
BssECI CCNNGG 8 cut(s) 168, 195, 1438, 1821, 2787, 2949, 3084, 3954
BssNI GRCGYC 1 cut(s) 351
BssSI CACGAG 1 cut(s) 2762
BssT1I CCWWGG 4 cut(s) 2787, 2949, 3084, 3954
Bst2BI CACGAG 1 cut(s) 2762
Bst2UI CCWGG 5 cut(s) 1440, 1823, 1937, 3290, 4013
Bst6I CTCTTC 5 cut(s) 440, 718, 2121, 2475, 2798
BstACI GRCGYC 1 cut(s) 351
BstAFI CTTAAG 1 cut(s) 3068
BstAPI GCANNNNNTGC 1 cut(s) 493
BstDSI CCRYGG 2 cut(s) 168, 195
BstFNI CGCG 2 cut(s) 170, 197
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 2 cut(s) 233, 394
BstMAI GTCTC 8 cut(s) 82, 504, 921, 1277, 1788, 2513, 2546, 3804
BstMCI CGRYCG 3 cut(s) 348, 485, 1740
BstNI CCWGG 5 cut(s) 1440, 1823, 1937, 3290, 4013
BstNSI RCATGY 3 cut(s) 562, 2835, 2866
BstPAI GACNNNNGTC 2 cut(s) 913, 2406
BstSCI CCNGG 9 cut(s) 281, 398, 453, 1438, 1821, 1935, 3288, 3665, 4011
BstSFI CTRYAG 6 cut(s) 1761, 2013, 2136, 2182, 2384, 3172
BstSLI GKGCMC 3 cut(s) 288, 2181, 2351
BstUI CGCG 2 cut(s) 170, 197
BstV2I GAAGAC 2 cut(s) 913, 2967
BstX2I RGATCY 3 cut(s) 372, 2148, 3527
BstXI CCANNNNNNTGG 2 cut(s) 2768, 3239
BstYI RGATCY 3 cut(s) 372, 2148, 3527
Bsu36I CCTNAGG 2 cut(s) 1227, 3009
BsuI GTATCC 1 cut(s) 2947
BtgI CCRYGG 2 cut(s) 168, 195
BtgZI GCGATG 1 cut(s) 202
BtsI GCAGTG 7 cut(s) 2022, 2053, 2179, 2564, 3181, 3616, 3765
BveI ACCTGC 2 cut(s) 1352, 3725
CaiI CAGNNNCTG 3 cut(s) 493, 1004, 1190
CfoI GCGC 2 cut(s) 233, 394
Cfr10I RCCGGY 4 cut(s) 388, 477, 739, 3659
Cfr13I GGNCC 9 cut(s) 243, 284, 285, 381, 1030, 1933, 2347, 2348, 2524
Cfr42I CCGCGG 2 cut(s) 171, 198
CpoI CGGWCCG 1 cut(s) 381
CseI GACGC 2 cut(s) 359, 1397
CsiI ACCWGGT 1 cut(s) 1935
Csp6I GTAC 6 cut(s) 36, 469, 1254, 2358, 2858, 3648
CspI CGGWCCG 1 cut(s) 381
CviQI GTAC 6 cut(s) 36, 469, 1254, 2358, 2858, 3648
DrdI GACNNNNNNGTC 2 cut(s) 356, 2560
DseDI GACNNNNNNGTC 2 cut(s) 356, 2560
EaeI YGGCCR 4 cut(s) 2418, 3229, 3456, 4003
Eam1104I CTCTTC 5 cut(s) 440, 718, 2121, 2475, 2798
EarI CTCTTC 5 cut(s) 440, 718, 2121, 2475, 2798
EciI GGCGGA 4 cut(s) 95, 119, 245, 1126
Ecl136II GAGCTC 2 cut(s) 103, 433
Eco130I CCWWGG 4 cut(s) 2787, 2949, 3084, 3954
Eco147I AGGCCT 2 cut(s) 2786, 3057
Eco24I GRGCYC 4 cut(s) 105, 288, 435, 2351
Eco31I GGTCTC 2 cut(s) 2546, 3804
Eco32I GATATC 1 cut(s) 3046
Eco47I GGWCC 3 cut(s) 243, 381, 1933
Eco53kI GAGCTC 2 cut(s) 103, 433
Eco57I CTGAAG 5 cut(s) 647, 873, 1435, 2979, 3769
Eco81I CCTNAGG 2 cut(s) 1227, 3009
Eco88I CYCGRG 1 cut(s) 368
EcoICRI GAGCTC 2 cut(s) 103, 433
EcoO109I RGGNCCY 3 cut(s) 2347, 2348, 2524
EcoRI GAATTC 2 cut(s) 586, 862
EcoRII CCWGG 5 cut(s) 1438, 1821, 1935, 3288, 4011
EcoRV GATATC 1 cut(s) 3046
EcoT14I CCWWGG 4 cut(s) 2787, 2949, 3084, 3954
EcoT22I ATGCAT 1 cut(s) 2367
EcoT38I GRGCYC 4 cut(s) 105, 288, 435, 2351
ErhI CCWWGG 4 cut(s) 2787, 2949, 3084, 3954
Esp3I CGTCTC 2 cut(s) 82, 504
FalI AAGNNNNNCTT 2 cut(s) 1308, 1340
FaqI GGGAC 3 cut(s) 490, 1227, 2587
FauI CCCGC 6 cut(s) 571, 599, 613, 1364, 1482, 3816
FauNDI CATATG 1 cut(s) 1297
FblI GTMKAC 3 cut(s) 2425, 2773, 3168
FriOI GRGCYC 4 cut(s) 105, 288, 435, 2351
GlaI GCGC 2 cut(s) 232, 393
GsaI CCCAGC 1 cut(s) 1787
GsuI CTGGAG 4 cut(s) 446, 656, 725, 2706
HaeII RGCGCY 1 cut(s) 395
HgaI GACGC 2 cut(s) 359, 1397
HhaI GCGC 2 cut(s) 233, 394
Hin1I GRCGYC 1 cut(s) 351
Hin6I GCGC 2 cut(s) 231, 392
HinP1I GCGC 2 cut(s) 231, 392
HincII GTYRAC 2 cut(s) 1828, 3807
HindII GTYRAC 2 cut(s) 1828, 3807
HphI GGTGA 3 cut(s) 1504, 2572, 3454
Hpy99I CGWCG 6 cut(s) 353, 533, 536, 539, 542, 545
HpyCH4IV ACGT 4 cut(s) 543, 2576, 2623, 2653
HpySE526I ACGT 4 cut(s) 543, 2576, 2623, 2653
Hsp92I GRCGYC 1 cut(s) 351
HspAI GCGC 2 cut(s) 231, 392
Kpn2I TCCGGA 2 cut(s) 240, 2588
KroI GCCGGC 2 cut(s) 388, 477
KroNI GCCGGC 2 cut(s) 390, 479
KspI CCGCGG 2 cut(s) 171, 198
LguI GCTCTTC 2 cut(s) 440, 2798
LweI GCATC 3 cut(s) 372, 3006, 3587
MabI ACCWGGT 1 cut(s) 1935
MaeII ACGT 4 cut(s) 543, 2576, 2623, 2653
MaeIII GTNAC 7 cut(s) 1273, 2068, 2562, 3316, 3460, 3631, 3985
MbiI CCGCTC 4 cut(s) 342, 606, 1177, 3522
MflI RGATCY 3 cut(s) 372, 2148, 3527
MhlI GDGCHC 6 cut(s) 105, 288, 435, 800, 2181, 2351
MlsI TGGCCA 2 cut(s) 2420, 3231
MluNI TGGCCA 2 cut(s) 2420, 3231
MlyI GAGTC 5 cut(s) 979, 1622, 1823, 1937, 3119
MmeI TCCRAC 2 cut(s) 732, 3726
Mox20I TGGCCA 2 cut(s) 2420, 3231
Mph1103I ATGCAT 1 cut(s) 2367
MreI CGCCGGCG 1 cut(s) 477
MroI TCCGGA 2 cut(s) 240, 2588
MroNI GCCGGC 2 cut(s) 388, 477
MroXI GAANNNNTTC 1 cut(s) 3159
MscI TGGCCA 2 cut(s) 2420, 3231
MslI CAYNNNNRTG 4 cut(s) 18, 563, 1088, 3237
Msp20I TGGCCA 2 cut(s) 2420, 3231
MspA1I CMGCKG 5 cut(s) 170, 197, 2012, 2123, 2720
MspCI CTTAAG 1 cut(s) 3068
MspR9I CCNGG 9 cut(s) 283, 400, 455, 1440, 1823, 1937, 3290, 3667, 4013
Mva1269I GAATGC 3 cut(s) 1911, 3273, 3702
MvaI CCWGG 5 cut(s) 1440, 1823, 1937, 3290, 4013
MvnI CGCG 2 cut(s) 170, 197
NaeI GCCGGC 2 cut(s) 390, 479
NciI CCSGG 4 cut(s) 283, 400, 455, 3667
NdeI CATATG 1 cut(s) 1297
NgoMIV GCCGGC 2 cut(s) 388, 477
NmeAIII GCCGAG 2 cut(s) 2481, 2884
NmuCI GTSAC 6 cut(s) 1273, 2068, 2562, 3460, 3631, 3985
NsiI ATGCAT 1 cut(s) 2367
NspI RCATGY 3 cut(s) 562, 2835, 2866
PaeI GCATGC 1 cut(s) 2835
PaeR7I CTCGAG 1 cut(s) 368
PceI AGGCCT 2 cut(s) 2786, 3057
PciI ACATGT 1 cut(s) 558
PciSI GCTCTTC 2 cut(s) 440, 2798
PcsI WCGNNNNNNNCGW 8 cut(s) 219, 354, 528, 534, 537, 540, 1639, 2064
PctI GAATGC 3 cut(s) 1911, 3273, 3702
PdiI GCCGGC 2 cut(s) 390, 479
PdmI GAANNNNTTC 1 cut(s) 3159
PfoI TCCNGGA 2 cut(s) 3288, 3665
Ple19I CGATCG 2 cut(s) 348, 485
PleI GAGTC 5 cut(s) 978, 1621, 1823, 1937, 3119
PpsI GAGTC 5 cut(s) 978, 1621, 1823, 1937, 3119
PscI ACATGT 1 cut(s) 558
PshAI GACNNNNGTC 2 cut(s) 913, 2406
PshBI ATTAAT 1 cut(s) 1707
PsiI TTATAA 1 cut(s) 2646
Psp124BI GAGCTC 2 cut(s) 105, 435
Psp6I CCWGG 5 cut(s) 1438, 1821, 1935, 3288, 4011
PspFI CCCAGC 1 cut(s) 1783
PspGI CCWGG 5 cut(s) 1438, 1821, 1935, 3288, 4011
PspOMI GGGCCC 2 cut(s) 284, 2347
PspPI GGNCC 9 cut(s) 243, 284, 285, 381, 1030, 1933, 2347, 2348, 2524
PsrI GAACNNNNNNTAC 2 cut(s) 3631, 3663
PstI CTGCAG 4 cut(s) 2017, 2140, 2186, 3176
PstNI CAGNNNCTG 3 cut(s) 493, 1004, 1190
PsuI RGATCY 3 cut(s) 372, 2148, 3527
PvuI CGATCG 2 cut(s) 348, 485
PvuII CAGCTG 2 cut(s) 2012, 2720
RgaI GCGATCGC 1 cut(s) 485
RsaI GTAC 6 cut(s) 37, 470, 1255, 2359, 2859, 3649
RsaNI GTAC 6 cut(s) 36, 469, 1254, 2358, 2858, 3648
RseI CAYNNNNRTG 4 cut(s) 18, 563, 1088, 3237
Rsr2I CGGWCCG 1 cut(s) 381
RsrII CGGWCCG 1 cut(s) 381
SacI GAGCTC 2 cut(s) 105, 435
SacII CCGCGG 2 cut(s) 171, 198
SapI GCTCTTC 2 cut(s) 440, 2798
Sau96I GGNCC 9 cut(s) 243, 284, 285, 381, 1030, 1933, 2347, 2348, 2524
ScaI AGTACT 1 cut(s) 37
SchI GAGTC 5 cut(s) 979, 1622, 1823, 1937, 3119
ScrFI CCNGG 9 cut(s) 283, 400, 455, 1440, 1823, 1937, 3290, 3667, 4013
SduI GDGCHC 6 cut(s) 105, 288, 435, 800, 2181, 2351
SexAI ACCWGGT 1 cut(s) 1935
SfaAI GCGATCGC 1 cut(s) 485
SfaNI GCATC 3 cut(s) 372, 3006, 3587
SfcI CTRYAG 6 cut(s) 1761, 2013, 2136, 2182, 2384, 3172
Sfr274I CTCGAG 1 cut(s) 368
Sfr303I CCGCGG 2 cut(s) 171, 198
SgfI GCGATCGC 1 cut(s) 485
SgrAI CRCCGGYG 1 cut(s) 477
SgrBI CCGCGG 2 cut(s) 171, 198
SinI GGWCC 3 cut(s) 243, 381, 1933
SlaI CTCGAG 1 cut(s) 368
SmiMI CAYNNNNRTG 4 cut(s) 18, 563, 1088, 3237
SmlI CTYRAG 6 cut(s) 368, 1280, 1403, 1607, 2397, 3068
SmoI CTYRAG 6 cut(s) 368, 1280, 1403, 1607, 2397, 3068
SpeI ACTAGT 3 cut(s) 32, 38, 1250
SphI GCATGC 1 cut(s) 2835
SseBI AGGCCT 2 cut(s) 2786, 3057
SspI AATATT 3 cut(s) 2518, 2937, 3222
SstI GAGCTC 2 cut(s) 105, 435
StuI AGGCCT 2 cut(s) 2786, 3057
StyD4I CCNGG 9 cut(s) 281, 398, 453, 1438, 1821, 1935, 3288, 3665, 4011
StyI CCWWGG 4 cut(s) 2787, 2949, 3084, 3954
TaiI ACGT 4 cut(s) 546, 2579, 2626, 2656
TaqI TCGA 8 cut(s) 344, 348, 369, 548, 584, 596, 1740, 3298
TaqII GACCGA 1 cut(s) 1726
TatI WGTACW 2 cut(s) 35, 2357
TauI GCSGC 9 cut(s) 71, 111, 135, 173, 197, 339, 342, 356, 645
TseFI GTSAC 6 cut(s) 1273, 2068, 2562, 3460, 3631, 3985
Tsp45I GTSAC 6 cut(s) 1273, 2068, 2562, 3460, 3631, 3985
TspGWI ACGGA 5 cut(s) 419, 843, 2990, 3198, 3227
Vha464I CTTAAG 1 cut(s) 3068
VpaK11BI GGWCC 3 cut(s) 243, 381, 1933
VspI ATTAAT 1 cut(s) 1707
XceI RCATGY 3 cut(s) 562, 2835, 2866
XcmI CCANNNNNNNNNTGG 1 cut(s) 1205
XhoI CTCGAG 1 cut(s) 368
XmiI GTMKAC 3 cut(s) 2425, 2773, 3168
XmnI GAANNNNTTC 1 cut(s) 3159
ZrmI AGTACT 1 cut(s) 37
Zsp2I ATGCAT 1 cut(s) 2367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.