FvH4_1g25720

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001358
Physical Location & Seq
Forward (+)
17608253 .. 17611183
2931 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25720.t1

Sequence Viewer

Length: 1440 bp
ATGGCGACCGCGTACCAACCAACGGGTGGAGCGGTGCCGGACTGGCTGAACAAAGGTGACAACGCATGGCAGATGATCTCGGCCACGCTGGTGGGTCTCCAGAGCGTGCCGGGGCTGGTCATCCTCTACGGGAGCGTAGTGAAGAAGAAATGGGCAGTCAACTCCGCCTTCATGGCACTCTACGCCTTCGCCGCCGTCGTCCTCTGCTGGGTATCGTGGGCTTACAAGATGTCGTTCGGGGACAAGCTGCTGCCGTTTTGGGGGAAGGCCGGACCTGCCTTAGGGCAGAAGTTTCTGCTCAAACAGGCTGCCCTCACCGCCACCACGCAGTACCACGCCAACGGCGAGCTTGAGACCCCCATGGTCACGCCGTGGTACCCGATGGCGACCATGGTGTGGTTTCAGTGTGTGTTTGCGGCCATAACACTGATACTGTTGGCAGGGTCGGTGTTGGGGAGAATGAACTTCAAGGCGTGGATGATGTTCGTGCCGCTGTGGCTGACGTTCTCGTACACCATCGGCGCGTTCAGCTTGTGGGGCGGAGGGTTCTTGTTCCACTGGGGTGTCATGGACTACTCCGGCGGATACGTCATTCATCTTTCTTCAGGGGTGGCTGGTTTCACCACTGCTTACTGGGTCGGACCTAGGGTGAAGAAGGACAGGGAGAGATTCCCACCAAACAATGTACTGCTGACATTGGCCGGAGCAGGGTTGCTGTGGATGGGATGGGCTGGTTTCAACGGCGGAGATCCGTACTCGGCCAACACAGACTCCTCCATGGCCGTCCTCAACACCAACATCTGCGCCGCAACGAGTCTCTTGGTTTGGACGTGGCTCGATGTCATCTTCTTCAACAAGCCCTCCGTCATCGGAGCTGTTCAGGGTATGATCACCGGCCTCGTCTGCATTACTCCGGCTGCAGGTCTTGTACAAGGATGGGCAGCAATAGTGATGGGCGTATTGTCAGGCAGCGTGCCATGGTTCACCATGATGATCGTAGACAAAAGGTGGAGACTCTTAACCGCAGTTGACGACACTCTAGGCGTTGTCCACACCCACGCAGTGGCCGGATTCTTAGGCGGTATATTGACCGGCATCTTTGCCGAGCCCCAACTATGTGCACTCTTCTTGCCGGTCACAAACTCTCGTGGGGTTGTCTACGGAGGAATCCAAGTCTTCAAACAAATCGTAGGCGGACTGTTTATCATCGGATGGAATATTGTGGTGACCTCGATCATTTGCATAGCAATTCGGTTTGTGATTCCTCTACGGATGCCGGAGGAGCAGTTGTTGATTGGGGATGACGCAGTGCATGGGGAAGAGGCCTATGCTTTGTGGGGCGATGGAGAGTTGTATGATGTTACTAGGCATGAACAGAATTACTCAGATGATCATACACAGGGTAATTTTGCTTCAAGTGGTGCCACTCAGGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

480

Amino Acids

51.99

Weight (kDa)

6.44

Isoelectric Point (pI)

26.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 22 - 443 8e-80 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 283, 911
Acc65I GGTACC 1 cut(s) 375
AccB1I GGYRCC 3 cut(s) 34, 375, 1421
AccB7I CCANNNNNTGG 3 cut(s) 26, 396, 1063
AccBSI CCGCTC 1 cut(s) 32
AccI GTMKAC 2 cut(s) 999, 1158
AccII CGCG 2 cut(s) 11, 524
AclWI GGATC 1 cut(s) 743
AcoI YGGCCR 6 cut(s) 81, 417, 699, 759, 780, 1065
AcuI CTGAAG 1 cut(s) 588
AdeI CACNNNGTG 2 cut(s) 372, 1063
AfaI GTAC 7 cut(s) 14, 332, 377, 512, 687, 755, 930
AfiI CCNNNNNNNGG 9 cut(s) 22, 26, 208, 260, 281, 396, 708, 920, 1063
AgsI TTSAA 5 cut(s) 469, 739, 853, 1180, 1416
AjiI CACGTC 1 cut(s) 831
AleI CACNNNNGTG 2 cut(s) 89, 561
AluBI AGCT 4 cut(s) 247, 349, 531, 875
AluI AGCT 4 cut(s) 247, 349, 531, 875
Alw21I GWGCWC 1 cut(s) 1123
Alw26I GTCTC 4 cut(s) 101, 347, 821, 1006
Alw44I GTGCAC 1 cut(s) 1119
AlwI GGATC 1 cut(s) 743
AoxI GGCC 9 cut(s) 81, 267, 417, 699, 759, 780, 895, 1065, 1323
ApaLI GTGCAC 1 cut(s) 1119
ApeKI GCWGC 6 cut(s) 247, 250, 308, 917, 941, 969
Asp718I GGTACC 1 cut(s) 375
AspA2I CCTAGG 1 cut(s) 644
AspLEI GCGC 2 cut(s) 524, 806
AspS9I GGNCC 2 cut(s) 272, 641
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 7 cut(s) 68, 307, 613, 661, 883, 976, 1237
AvaII GGWCC 2 cut(s) 272, 641
AvrII CCTAGG 1 cut(s) 644
AxyI CCTNAGG 1 cut(s) 280
BaeGI GKGCMC 1 cut(s) 1123
BanI GGYRCC 3 cut(s) 34, 375, 1421
BanII GRGCYC 1 cut(s) 1110
BarI GAAGNNNNNNTAC 2 cut(s) 1396, 1428
BauI CACGAG 1 cut(s) 1146
BbsI GAAGAC 1 cut(s) 1168
Bbv12I GWGCWC 1 cut(s) 1123
BbvI GCAGC 6 cut(s) 234, 237, 295, 904, 953, 981
BccI CCATC 8 cut(s) 376, 524, 715, 720, 930, 946, 1206, 1337
BceAI ACGGC 6 cut(s) 179, 238, 355, 358, 757, 767
BciVI GTATCC 1 cut(s) 578
BclI TGATCA 2 cut(s) 888, 1390
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 4 cut(s) 101, 347, 821, 1006
BfaI CTAG 3 cut(s) 645, 1040, 1365
BfmI CTRYAG 1 cut(s) 918
BfuAI ACCTGC 2 cut(s) 283, 911
BfuI GTATCC 1 cut(s) 578
BglI GCCNNNNNGGC 3 cut(s) 43, 173, 496
BlnI CCTAGG 1 cut(s) 644
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 2 cut(s) 272, 641
BmgBI CACGTC 1 cut(s) 831
BmgT120I GGNCC 2 cut(s) 272, 641
BmiI GGNNCC 3 cut(s) 36, 377, 1423
BmrFI CCNGG 1 cut(s) 111
BmrI ACTGGG 2 cut(s) 568, 643
BmsI GCATC 2 cut(s) 1104, 1263
BmuI ACTGGG 2 cut(s) 568, 643
BpiI GAAGAC 1 cut(s) 1168
BpmI CTGGAG 1 cut(s) 83
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 111
BsaI GGTCTC 2 cut(s) 101, 347
BsaJI CCNNGG 7 cut(s) 110, 360, 371, 390, 644, 777, 977
BsaXI ACNNNNNCTCC 8 cut(s) 755, 785, 845, 875, 1271, 1274, 1301, 1304
Bsc4I CCNNNNNNNGG 9 cut(s) 22, 26, 208, 260, 281, 396, 708, 920, 1063
Bse118I RCCGGY 3 cut(s) 893, 1091, 1132
Bse1I ACTGG 3 cut(s) 47, 563, 638
Bse21I CCTNAGG 1 cut(s) 280
BseDI CCNNGG 7 cut(s) 110, 360, 371, 390, 644, 777, 977
BseGI GGATG 8 cut(s) 120, 483, 726, 731, 941, 1217, 1278, 1306
BseLI CCNNNNNNNGG 9 cut(s) 22, 26, 208, 260, 281, 396, 708, 920, 1063
BseMII CTCAG 1 cut(s) 1398
BseNI ACTGG 3 cut(s) 47, 563, 638
BseRI GAGGAG 2 cut(s) 763, 1295
BseSI GKGCMC 1 cut(s) 1123
BseXI GCAGC 6 cut(s) 234, 237, 295, 904, 953, 981
BseYI CCCAGC 1 cut(s) 207
Bsh1236I CGCG 2 cut(s) 11, 524
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 9 cut(s) 83, 269, 419, 701, 761, 782, 897, 1067, 1325
BshNI GGYRCC 3 cut(s) 34, 375, 1421
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 1123
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 9 cut(s) 22, 26, 208, 260, 281, 396, 708, 920, 1063
BsmAI GTCTC 4 cut(s) 101, 347, 821, 1006
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 9 cut(s) 83, 269, 419, 701, 761, 782, 897, 1067, 1325
Bso31I GGTCTC 2 cut(s) 101, 347
Bsp1286I GDGCHC 2 cut(s) 1110, 1123
Bsp1407I TGTACA 1 cut(s) 928
Bsp143I GATC 6 cut(s) 75, 748, 888, 993, 1233, 1390
Bsp19I CCATGG 4 cut(s) 360, 390, 777, 977
BspANI GGCC 9 cut(s) 83, 269, 419, 701, 761, 782, 897, 1067, 1325
BspCNI CTCAG 1 cut(s) 1397
BspFNI CGCG 2 cut(s) 11, 524
BspLI GGNNCC 3 cut(s) 36, 377, 1423
BspMAI CTGCAG 1 cut(s) 922
BspMI ACCTGC 2 cut(s) 283, 911
BspPI GGATC 1 cut(s) 743
BspT107I GGYRCC 3 cut(s) 34, 375, 1421
BspTNI GGTCTC 2 cut(s) 101, 347
BsrBI CCGCTC 1 cut(s) 32
BsrFI RCCGGY 3 cut(s) 893, 1091, 1132
BsrGI TGTACA 1 cut(s) 928
BsrI ACTGG 3 cut(s) 47, 563, 638
BssAI RCCGGY 3 cut(s) 893, 1091, 1132
BssECI CCNNGG 7 cut(s) 110, 360, 371, 390, 644, 777, 977
BssMI GATC 6 cut(s) 75, 748, 888, 993, 1233, 1390
BssSI CACGAG 1 cut(s) 1146
BssT1I CCWWGG 5 cut(s) 360, 390, 644, 777, 977
Bst2BI CACGAG 1 cut(s) 1146
Bst4CI ACNGT 2 cut(s) 435, 1200
Bst6I CTCTTC 2 cut(s) 1130, 1314
BstAUI TGTACA 1 cut(s) 928
BstC8I GCNNGC 3 cut(s) 107, 347, 974
BstDEI CTNAG 4 cut(s) 280, 1075, 1384, 1428
BstDSI CCRYGG 5 cut(s) 360, 371, 390, 777, 977
BstEII GGTNACC 1 cut(s) 1225
BstENI CCTNNNNNAGG 1 cut(s) 279
BstF5I GGATG 8 cut(s) 120, 483, 726, 731, 941, 1217, 1278, 1306
BstFNI CGCG 2 cut(s) 11, 524
BstHHI GCGC 2 cut(s) 524, 806
BstKTI GATC 6 cut(s) 78, 751, 891, 996, 1236, 1393
BstMAI GTCTC 4 cut(s) 101, 347, 821, 1006
BstMBI GATC 6 cut(s) 75, 748, 888, 993, 1233, 1390
BstMCI CGRYCG 1 cut(s) 9
BstPI GGTNACC 1 cut(s) 1225
BstSCI CCNGG 1 cut(s) 109
BstSFI CTRYAG 1 cut(s) 918
BstSLI GKGCMC 1 cut(s) 1123
BstUI CGCG 2 cut(s) 11, 524
BstV1I GCAGC 6 cut(s) 234, 237, 295, 904, 953, 981
BstV2I GAAGAC 1 cut(s) 1168
BstX2I RGATCY 1 cut(s) 748
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 1 cut(s) 748
Bsu36I CCTNAGG 1 cut(s) 280
BsuI GTATCC 1 cut(s) 578
BsuRI GGCC 9 cut(s) 83, 269, 419, 701, 761, 782, 897, 1067, 1325
BtgI CCRYGG 5 cut(s) 360, 371, 390, 777, 977
BtgZI GCGATG 1 cut(s) 1356
BtrI CACGTC 1 cut(s) 831
BtsCI GGATG 8 cut(s) 120, 483, 726, 731, 941, 1217, 1278, 1306
BtsI GCAGTG 3 cut(s) 624, 1068, 1314
BtsIMutI CAGTG 6 cut(s) 410, 425, 556, 624, 1068, 1314
BveI ACCTGC 2 cut(s) 283, 911
Cac8I GCNNGC 3 cut(s) 107, 347, 974
CfoI GCGC 2 cut(s) 524, 806
Cfr10I RCCGGY 3 cut(s) 893, 1091, 1132
Cfr13I GGNCC 2 cut(s) 272, 641
CseI GACGC 1 cut(s) 1313
Csp6I GTAC 7 cut(s) 13, 331, 376, 511, 686, 754, 929
CspCI CAANNNNNGTGG 1 cut(s) 1414
CviQI GTAC 7 cut(s) 13, 331, 376, 511, 686, 754, 929
DdeI CTNAG 4 cut(s) 280, 1075, 1384, 1428
DpnI GATC 6 cut(s) 77, 750, 890, 995, 1235, 1392
DpnII GATC 6 cut(s) 75, 748, 888, 993, 1233, 1390
DraIII CACNNNGTG 2 cut(s) 372, 1063
EaeI YGGCCR 6 cut(s) 81, 417, 699, 759, 780, 1065
Eam1104I CTCTTC 2 cut(s) 1130, 1314
EarI CTCTTC 2 cut(s) 1130, 1314
EciI GGCGGA 5 cut(s) 154, 555, 597, 759, 1209
Eco130I CCWWGG 5 cut(s) 360, 390, 644, 777, 977
Eco147I AGGCCT 1 cut(s) 1325
Eco24I GRGCYC 1 cut(s) 1110
Eco31I GGTCTC 2 cut(s) 101, 347
Eco47I GGWCC 2 cut(s) 272, 641
Eco57I CTGAAG 1 cut(s) 588
Eco81I CCTNAGG 1 cut(s) 280
Eco91I GGTNACC 1 cut(s) 1225
EcoNI CCTNNNNNAGG 1 cut(s) 279
EcoO65I GGTNACC 1 cut(s) 1225
EcoT14I CCWWGG 5 cut(s) 360, 390, 644, 777, 977
EcoT38I GRGCYC 1 cut(s) 1110
ErhI CCWWGG 5 cut(s) 360, 390, 644, 777, 977
FaqI GGGAC 1 cut(s) 254
FbaI TGATCA 2 cut(s) 888, 1390
FblI GTMKAC 2 cut(s) 999, 1158
FokI GGATG 8 cut(s) 107, 490, 733, 738, 948, 1224, 1285, 1313
FriOI GRGCYC 1 cut(s) 1110
FspBI CTAG 3 cut(s) 645, 1040, 1365
GlaI GCGC 2 cut(s) 523, 805
GsaI CCCAGC 1 cut(s) 211
GsuI CTGGAG 1 cut(s) 83
HaeIII GGCC 9 cut(s) 83, 269, 419, 701, 761, 782, 897, 1067, 1325
HgaI GACGC 1 cut(s) 1313
HhaI GCGC 2 cut(s) 524, 806
Hin6I GCGC 2 cut(s) 522, 804
HinP1I GCGC 2 cut(s) 522, 804
HincII GTYRAC 2 cut(s) 160, 1030
HindII GTYRAC 2 cut(s) 160, 1030
HinfI GANTC 7 cut(s) 669, 770, 814, 1014, 1071, 1167, 1261
HphI GGTGA 7 cut(s) 68, 307, 613, 661, 883, 976, 1237
Hpy166II GTNNAC 8 cut(s) 160, 513, 984, 1000, 1030, 1051, 1121, 1159
Hpy188I TCNGA 4 cut(s) 641, 872, 1211, 1387
Hpy188III TCNNGA 1 cut(s) 100
Hpy8I GTNNAC 8 cut(s) 160, 513, 984, 1000, 1030, 1051, 1121, 1159
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 4 cut(s) 178, 196, 259, 649
HpyCH4III ACNGT 2 cut(s) 435, 1200
HpyCH4IV ACGT 3 cut(s) 503, 588, 830
HpyCH4V TGCA 5 cut(s) 906, 920, 1121, 1242, 1312
HpyF3I CTNAG 4 cut(s) 280, 1075, 1384, 1428
HpySE526I ACGT 3 cut(s) 503, 588, 830
HspAI GCGC 2 cut(s) 522, 804
KpnI GGTACC 1 cut(s) 379
Ksp22I TGATCA 2 cut(s) 888, 1390
Kzo9I GATC 6 cut(s) 75, 748, 888, 993, 1233, 1390
LmnI GCTCC 5 cut(s) 29, 132, 704, 872, 1282
Lsp1109I GCAGC 6 cut(s) 234, 237, 295, 904, 953, 981
LweI GCATC 2 cut(s) 1104, 1263
MaeI CTAG 3 cut(s) 645, 1040, 1365
MaeII ACGT 3 cut(s) 503, 588, 830
MaeIII GTNAC 5 cut(s) 56, 364, 1135, 1225, 1360
MalI GATC 6 cut(s) 77, 750, 890, 995, 1235, 1392
MbiI CCGCTC 1 cut(s) 32
MboI GATC 6 cut(s) 75, 748, 888, 993, 1233, 1390
MboII GAAGA 9 cut(s) 154, 157, 594, 664, 838, 841, 1117, 1168, 1331
MflI RGATCY 1 cut(s) 748
MhlI GDGCHC 2 cut(s) 1110, 1123
MluCI AATT 3 cut(s) 1248, 1378, 1405
MlyI GAGTC 3 cut(s) 764, 823, 1008
MmeI TCCRAC 1 cut(s) 619
MseI TTAA 1 cut(s) 1019
MslI CAYNNNNRTG 3 cut(s) 89, 561, 989
MspA1I CMGCKG 1 cut(s) 493
MspR9I CCNGG 1 cut(s) 111
MvnI CGCG 2 cut(s) 11, 524
NciI CCSGG 1 cut(s) 111
NcoI CCATGG 4 cut(s) 360, 390, 777, 977
NdeII GATC 6 cut(s) 75, 748, 888, 993, 1233, 1390
NlaIV GGNNCC 3 cut(s) 36, 377, 1423
NmeAIII GCCGAG 3 cut(s) 59, 737, 1129
NmuCI GTSAC 4 cut(s) 56, 364, 1135, 1225
OliI CACNNNNGTG 2 cut(s) 89, 561
PceI AGGCCT 1 cut(s) 1325
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 342
PfeI GAWTC 4 cut(s) 669, 1071, 1167, 1261
PflMI CCANNNNNTGG 3 cut(s) 26, 396, 1063
PleI GAGTC 3 cut(s) 764, 822, 1008
PpsI GAGTC 3 cut(s) 764, 822, 1008
PspEI GGTNACC 1 cut(s) 1225
PspFI CCCAGC 1 cut(s) 207
PspN4I GGNNCC 3 cut(s) 36, 377, 1423
PspPI GGNCC 2 cut(s) 272, 641
PstI CTGCAG 1 cut(s) 922
PsuI RGATCY 1 cut(s) 748
RsaI GTAC 7 cut(s) 14, 332, 377, 512, 687, 755, 930
RsaNI GTAC 7 cut(s) 13, 331, 376, 511, 686, 754, 929
RseI CAYNNNNRTG 3 cut(s) 89, 561, 989
SaqAI TTAA 1 cut(s) 1019
Sau3AI GATC 6 cut(s) 75, 748, 888, 993, 1233, 1390
Sau96I GGNCC 2 cut(s) 272, 641
SchI GAGTC 3 cut(s) 764, 823, 1008
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 2 cut(s) 1110, 1123
SfaNI GCATC 2 cut(s) 1104, 1263
SfcI CTRYAG 1 cut(s) 918
SinI GGWCC 2 cut(s) 272, 641
SmiMI CAYNNNNRTG 3 cut(s) 89, 561, 989
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 3 cut(s) 1248, 1378, 1405
SseBI AGGCCT 1 cut(s) 1325
SspI AATATT 1 cut(s) 1219
SspMI CTAG 3 cut(s) 645, 1040, 1365
StuI AGGCCT 1 cut(s) 1325
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 5 cut(s) 360, 390, 644, 777, 977
TaaI ACNGT 2 cut(s) 435, 1200
TaiI ACGT 3 cut(s) 506, 591, 833
TaqI TCGA 2 cut(s) 837, 1232
TasI AATT 3 cut(s) 1248, 1378, 1405
TatI WGTACW 2 cut(s) 685, 928
TauI GCSGC 4 cut(s) 194, 419, 493, 809
TfiI GAWTC 4 cut(s) 669, 1071, 1167, 1261
Tru1I TTAA 1 cut(s) 1019
Tru9I TTAA 1 cut(s) 1019
TscAI CASTG 6 cut(s) 410, 432, 563, 631, 1068, 1314
TseFI GTSAC 4 cut(s) 56, 364, 1135, 1225
TseI GCWGC 6 cut(s) 247, 250, 308, 917, 941, 969
Tsp45I GTSAC 4 cut(s) 56, 364, 1135, 1225
TspDTI ATGAA 4 cut(s) 160, 476, 584, 1386
TspGWI ACGGA 4 cut(s) 741, 853, 1176, 1285
TspRI CASTG 6 cut(s) 410, 432, 563, 631, 1068, 1314
Van91I CCANNNNNTGG 3 cut(s) 26, 396, 1063
VneI GTGCAC 1 cut(s) 1119
VpaK11BI GGWCC 2 cut(s) 272, 641
XagI CCTNNNNNAGG 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XmaJI CCTAGG 1 cut(s) 644
XmiI GTMKAC 2 cut(s) 999, 1158
XspI CTAG 3 cut(s) 645, 1040, 1365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.