Prupe.4G285000_v2.0.a1

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001358
Physical Location & Seq
Forward (+)
24739497 .. 24742452
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G285000.1

Sequence Viewer

Length: 939 bp
ATGGCAGATTTTATCGCGGTTGAGCTAGAAAAAGGACGTAGCGGGATCTGGATAAAGGAACTTGATTTGCTTGTGCTGCGTCACTATTGCTCGTTTTCTTTCTCTCCATTAGTCAACGTCTACTGGCCTGGACCAAGGGTGAAGAAGGACAGGGAGAGATTTCCACCAAACAACGTACTTCTGGTGTTAGCAGGAGCCGGGTTGCTGTGGATGGGATGGGCAGGTTTCAACGGGGGAGACCCGTACGTGGCAAACACGGACTCATCCATGGCCGTCCTCAACACCAACATCTGCGCCGCCACGAGCTTCTTGGTTTGGACTTGGCTCGATGTCATCTTCTTTGAGAAGCCTTCCGTCATTGGAGCTGTCCAAGGAATGATCACTGGCCTCGTCTGCATTACTCCTGCTGCAGGTCTGGTTCAAGGATGGGCCGCTATAGTGATGGGAGTTATGTCAGGCAGTGTGCCATGGTTCACCATGATGATAGTGGACAAGAGGTGGAAACTGCTATCTGCAGTTGACGACACCCTTGGTGTAGTTCACACCCACGCCGTGGCCGGGTTTCTTGGCGGCGTTTTAACGGGGCTTTTCGCTGAGCCTGAACTATGTGCACTCTTTTTGCCCGTCACAAACTCGCGCGGAGGCGTGTACGGAGGCTCAGGATTAATGCAAATCCTCAAACAAATTGTGGGAGCCCTATTTATCATTGGATGGAATATTGTGGTCACCTCCATTATTTGCCTAGTCTTACGGTGTATAGTTCCGTTGCGCATGCCGGAGGAACAGTTACTGATTGGGGATGACGCAGTGCATGGAGAAGAGGCATATGCATTGTGGGGTGATGGGGAGAAGTATGATGTTACTAGGCATGAGCTTTACTCCGACGATACGTCACACGCTCAAAAATATCCATCCAGTGGTGCCACTCAGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

313

Amino Acids

33.89

Weight (kDa)

5.21

Isoelectric Point (pI)

32.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 770
Acc36I ACCTGC 2 cut(s) 212, 401
AccB1I GGYRCC 1 cut(s) 920
AccB7I CCANNNNNTGG 2 cut(s) 553, 917
AccI GTMKAC 1 cut(s) 120
AccII CGCG 3 cut(s) 17, 637, 639
AciI CCGC 6 cut(s) 17, 42, 297, 432, 570, 639
AclWI GGATC 1 cut(s) 53
AcoI YGGCCR 2 cut(s) 270, 555
AdeI CACNNNGTG 1 cut(s) 553
AfaI GTAC 3 cut(s) 177, 245, 650
AfiI CCNNNNNNNGG 5 cut(s) 247, 410, 553, 558, 917
AgsI TTSAA 2 cut(s) 229, 422
AhdI GACNNNNNGTC 1 cut(s) 889
AjnI CCWGG 1 cut(s) 127
AleI CACNNNNGTG 1 cut(s) 929
AluBI AGCT 4 cut(s) 25, 306, 365, 874
AluI AGCT 4 cut(s) 25, 306, 365, 874
Alw21I GWGCWC 1 cut(s) 613
Alw26I GTCTC 1 cut(s) 231
Alw44I GTGCAC 1 cut(s) 609
AlwI GGATC 1 cut(s) 53
AlwNI CAGNNNCTG 1 cut(s) 790
AoxI GGCC 5 cut(s) 125, 270, 385, 429, 555
ApaLI GTGCAC 1 cut(s) 609
ApeKI GCWGC 2 cut(s) 76, 407
AseI ATTAAT 1 cut(s) 665
AspLEI GCGC 3 cut(s) 296, 639, 771
AspS9I GGNCC 2 cut(s) 131, 429
AsuC2I CCSGG 2 cut(s) 199, 559
AsuHPI GGTGA 4 cut(s) 151, 466, 718, 851
AvaII GGWCC 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 613
BanI GGYRCC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 697
BauI CACGAG 1 cut(s) 301
Bbv12I GWGCWC 1 cut(s) 613
BbvI GCAGC 2 cut(s) 63, 394
BccI CCATC 7 cut(s) 205, 210, 420, 436, 705, 836, 919
BceAI ACGGC 2 cut(s) 257, 536
BciT130I CCWGG 1 cut(s) 129
BclI TGATCA 1 cut(s) 378
BcnI CCSGG 2 cut(s) 199, 559
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 3 cut(s) 26, 743, 864
BfmI CTRYAG 3 cut(s) 408, 435, 513
BfuAI ACCTGC 2 cut(s) 212, 401
BisI GCNGC 5 cut(s) 77, 297, 408, 432, 571
BlpI GCTNAGC 1 cut(s) 594
BlsI GCNGC 5 cut(s) 78, 298, 409, 433, 572
Bme1390I CCNGG 3 cut(s) 129, 199, 559
Bme18I GGWCC 1 cut(s) 131
BmeRI GACNNNNNGTC 1 cut(s) 889
BmgT120I GGNCC 2 cut(s) 131, 429
BmiI GGNNCC 3 cut(s) 196, 694, 922
BmrFI CCNGG 3 cut(s) 129, 199, 559
BplI GAGNNNNNCTC 2 cut(s) 863, 895
Bpu10I CCTNAGC 1 cut(s) 658
Bpu1102I GCTNAGC 1 cut(s) 594
BpuMI CCSGG 2 cut(s) 199, 559
BsaAI YACGTR 1 cut(s) 247
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 6 cut(s) 134, 267, 370, 467, 529, 552
BsaXI ACNNNNNCTCC 2 cut(s) 770, 800
Bsc4I CCNNNNNNNGG 5 cut(s) 247, 410, 553, 558, 917
Bse1I ACTGG 3 cut(s) 128, 388, 915
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 6 cut(s) 134, 267, 370, 467, 529, 552
BseGI GGATG 7 cut(s) 216, 221, 263, 431, 716, 805, 911
BseLI CCNNNNNNNGG 5 cut(s) 247, 410, 553, 558, 917
BseMII CTCAG 2 cut(s) 585, 672
BseNI ACTGG 3 cut(s) 128, 388, 915
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 2 cut(s) 63, 394
Bsh1236I CGCG 3 cut(s) 17, 637, 639
BshFI GGCC 5 cut(s) 127, 272, 387, 431, 557
BshNI GGYRCC 1 cut(s) 920
BsiHKAI GWGCWC 1 cut(s) 613
BsiSI CCGG 3 cut(s) 198, 558, 776
BsiWI CGTACG 1 cut(s) 243
BslI CCNNNNNNNGG 5 cut(s) 247, 410, 553, 558, 917
BsmAI GTCTC 1 cut(s) 231
BsnI GGCC 5 cut(s) 127, 272, 387, 431, 557
Bso31I GGTCTC 1 cut(s) 231
Bsp1286I GDGCHC 2 cut(s) 613, 697
Bsp143I GATC 2 cut(s) 45, 378
Bsp1720I GCTNAGC 1 cut(s) 594
Bsp19I CCATGG 2 cut(s) 267, 467
BspACI CCGC 6 cut(s) 17, 42, 297, 432, 570, 639
BspANI GGCC 5 cut(s) 127, 272, 387, 431, 557
BspCNI CTCAG 2 cut(s) 586, 671
BspFNI CGCG 3 cut(s) 17, 637, 639
BspLI GGNNCC 3 cut(s) 196, 694, 922
BspMAI CTGCAG 2 cut(s) 412, 517
BspMI ACCTGC 2 cut(s) 212, 401
BspPI GGATC 1 cut(s) 53
BspT107I GGYRCC 1 cut(s) 920
BspTNI GGTCTC 1 cut(s) 231
BsrI ACTGG 3 cut(s) 128, 388, 915
BssECI CCNNGG 6 cut(s) 134, 267, 370, 467, 529, 552
BssMI GATC 2 cut(s) 45, 378
BssSI CACGAG 1 cut(s) 301
BssT1I CCWWGG 5 cut(s) 134, 267, 370, 467, 529
Bst2BI CACGAG 1 cut(s) 301
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 2 cut(s) 753, 786
Bst6I CTCTTC 1 cut(s) 813
BstBAI YACGTR 1 cut(s) 247
BstC8I GCNNGC 1 cut(s) 773
BstDEI CTNAG 3 cut(s) 594, 658, 927
BstDSI CCRYGG 3 cut(s) 267, 467, 552
BstEII GGTNACC 1 cut(s) 724
BstENI CCTNNNNNAGG 1 cut(s) 408
BstF5I GGATG 7 cut(s) 216, 221, 263, 431, 716, 805, 911
BstFNI CGCG 3 cut(s) 17, 637, 639
BstHHI GCGC 3 cut(s) 296, 639, 771
BstKTI GATC 2 cut(s) 48, 381
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 2 cut(s) 45, 378
BstMWI GCNNNNNNNGC 2 cut(s) 76, 393
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 775
BstPI GGTNACC 1 cut(s) 724
BstSCI CCNGG 3 cut(s) 127, 197, 557
BstSFI CTRYAG 3 cut(s) 408, 435, 513
BstSLI GKGCMC 1 cut(s) 613
BstUI CGCG 3 cut(s) 17, 637, 639
BstV1I GCAGC 2 cut(s) 63, 394
BstX2I RGATCY 1 cut(s) 45
BstXI CCANNNNNNTGG 1 cut(s) 931
BstYI RGATCY 1 cut(s) 45
BsuRI GGCC 5 cut(s) 127, 272, 387, 431, 557
BtgI CCRYGG 3 cut(s) 267, 467, 552
BtsCI GGATG 7 cut(s) 216, 221, 263, 431, 716, 805, 911
BtsI GCAGTG 2 cut(s) 466, 813
BtsIMutI CAGTG 4 cut(s) 381, 466, 813, 922
BveI ACCTGC 2 cut(s) 212, 401
Cac8I GCNNGC 1 cut(s) 773
CaiI CAGNNNCTG 1 cut(s) 790
CfoI GCGC 3 cut(s) 296, 639, 771
Cfr13I GGNCC 2 cut(s) 131, 429
CseI GACGC 2 cut(s) 68, 812
Csp6I GTAC 3 cut(s) 176, 244, 649
CviAII CATG 6 cut(s) 268, 468, 478, 772, 812, 869
CviQI GTAC 3 cut(s) 176, 244, 649
DdeI CTNAG 3 cut(s) 594, 658, 927
DpnI GATC 2 cut(s) 47, 380
DpnII GATC 2 cut(s) 45, 378
DraIII CACNNNGTG 1 cut(s) 553
DriI GACNNNNNGTC 1 cut(s) 889
EaeI YGGCCR 2 cut(s) 270, 555
Eam1104I CTCTTC 1 cut(s) 813
Eam1105I GACNNNNNGTC 1 cut(s) 889
EarI CTCTTC 1 cut(s) 813
Eco130I CCWWGG 5 cut(s) 134, 267, 370, 467, 529
Eco24I GRGCYC 1 cut(s) 697
Eco31I GGTCTC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 131
Eco91I GGTNACC 1 cut(s) 724
EcoNI CCTNNNNNAGG 1 cut(s) 408
EcoO65I GGTNACC 1 cut(s) 724
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 5 cut(s) 134, 267, 370, 467, 529
EcoT22I ATGCAT 1 cut(s) 832
EcoT38I GRGCYC 1 cut(s) 697
ErhI CCWWGG 5 cut(s) 134, 267, 370, 467, 529
FaeI CATG 6 cut(s) 271, 471, 481, 775, 815, 872
FatI CATG 6 cut(s) 267, 467, 477, 771, 811, 868
FauI CCCGC 1 cut(s) 35
FauNDI CATATG 1 cut(s) 826
FbaI TGATCA 1 cut(s) 378
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 5 cut(s) 77, 297, 408, 432, 571
FokI GGATG 7 cut(s) 223, 228, 250, 438, 723, 812, 898
FriOI GRGCYC 1 cut(s) 697
Fsp4HI GCNGC 5 cut(s) 77, 297, 408, 432, 571
FspBI CTAG 3 cut(s) 26, 743, 864
FspI TGCGCA 1 cut(s) 770
GlaI GCGC 3 cut(s) 295, 638, 770
GluI GCNGC 5 cut(s) 77, 297, 408, 432, 571
HaeIII GGCC 5 cut(s) 127, 272, 387, 431, 557
HapII CCGG 3 cut(s) 198, 558, 776
HgaI GACGC 2 cut(s) 68, 812
HhaI GCGC 3 cut(s) 296, 639, 771
Hin1II CATG 6 cut(s) 271, 471, 481, 775, 815, 872
Hin6I GCGC 3 cut(s) 294, 637, 769
HinP1I GCGC 3 cut(s) 294, 637, 769
HincII GTYRAC 2 cut(s) 115, 520
HindII GTYRAC 2 cut(s) 115, 520
HinfI GANTC 1 cut(s) 260
HpaII CCGG 3 cut(s) 198, 558, 776
HphI GGTGA 4 cut(s) 151, 466, 718, 851
Hpy166II GTNNAC 8 cut(s) 115, 121, 474, 490, 520, 541, 611, 649
Hpy188I TCNGA 1 cut(s) 883
Hpy188III TCNNGA 2 cut(s) 49, 660
Hpy8I GTNNAC 8 cut(s) 115, 121, 474, 490, 520, 541, 611, 649
Hpy99I CGWCG 1 cut(s) 887
HpyAV CCTTC 2 cut(s) 139, 360
HpyCH4III ACNGT 2 cut(s) 753, 786
HpyCH4IV ACGT 5 cut(s) 37, 117, 174, 246, 890
HpyCH4V TGCA 7 cut(s) 396, 410, 515, 611, 670, 811, 830
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 393
HpyF3I CTNAG 3 cut(s) 594, 658, 927
HpySE526I ACGT 5 cut(s) 37, 117, 174, 246, 890
Hsp92II CATG 6 cut(s) 271, 471, 481, 775, 815, 872
HspAI GCGC 3 cut(s) 294, 637, 769
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 2 cut(s) 45, 378
LmnI GCTCC 3 cut(s) 194, 362, 692
Lsp1109I GCAGC 2 cut(s) 63, 394
MaeI CTAG 3 cut(s) 26, 743, 864
MaeII ACGT 5 cut(s) 37, 117, 174, 246, 890
MaeIII GTNAC 6 cut(s) 80, 625, 724, 786, 859, 891
MalI GATC 2 cut(s) 47, 380
MboI GATC 2 cut(s) 45, 378
MboII GAAGA 3 cut(s) 154, 328, 830
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 2 cut(s) 613, 697
MluCI AATT 1 cut(s) 684
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 906
MnlI CCTC 9 cut(s) 287, 398, 489, 635, 647, 686, 739, 772, 814
Mph1103I ATGCAT 1 cut(s) 832
MseI TTAA 2 cut(s) 578, 665
MslI CAYNNNNRTG 2 cut(s) 479, 929
MspI CCGG 3 cut(s) 198, 558, 776
MspR9I CCNGG 3 cut(s) 129, 199, 559
MvaI CCWGG 1 cut(s) 129
MvnI CGCG 3 cut(s) 17, 637, 639
MwoI GCNNNNNNNGC 2 cut(s) 76, 393
NciI CCSGG 2 cut(s) 199, 559
NcoI CCATGG 2 cut(s) 267, 467
NdeI CATATG 1 cut(s) 826
NdeII GATC 2 cut(s) 45, 378
NlaIII CATG 6 cut(s) 271, 471, 481, 775, 815, 872
NlaIV GGNNCC 3 cut(s) 196, 694, 922
NmuCI GTSAC 4 cut(s) 80, 625, 724, 891
NsbI TGCGCA 1 cut(s) 770
NsiI ATGCAT 1 cut(s) 832
NspI RCATGY 1 cut(s) 775
OliI CACNNNNGTG 1 cut(s) 929
PaeI GCATGC 1 cut(s) 775
Pfl23II CGTACG 1 cut(s) 243
PflMI CCANNNNNTGG 2 cut(s) 553, 917
PkrI GCNGC 5 cut(s) 78, 298, 409, 433, 572
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
Ppu21I YACGTR 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 665
Psp6I CCWGG 1 cut(s) 127
PspEI GGTNACC 1 cut(s) 724
PspGI CCWGG 1 cut(s) 127
PspLI CGTACG 1 cut(s) 243
PspN4I GGNNCC 3 cut(s) 196, 694, 922
PspPI GGNCC 2 cut(s) 131, 429
PstI CTGCAG 2 cut(s) 412, 517
PstNI CAGNNNCTG 1 cut(s) 790
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 3 cut(s) 177, 245, 650
RsaNI GTAC 3 cut(s) 176, 244, 649
RseI CAYNNNNRTG 2 cut(s) 479, 929
SaqAI TTAA 2 cut(s) 578, 665
SatI GCNGC 5 cut(s) 77, 297, 408, 432, 571
Sau3AI GATC 2 cut(s) 45, 378
Sau96I GGNCC 2 cut(s) 131, 429
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 3 cut(s) 129, 199, 559
SduI GDGCHC 2 cut(s) 613, 697
SfcI CTRYAG 3 cut(s) 408, 435, 513
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 2 cut(s) 479, 929
SphI GCATGC 1 cut(s) 775
Sse9I AATT 1 cut(s) 684
SsiI CCGC 6 cut(s) 17, 42, 297, 432, 570, 639
SspI AATATT 1 cut(s) 718
SspMI CTAG 3 cut(s) 26, 743, 864
StyD4I CCNGG 3 cut(s) 127, 197, 557
StyI CCWWGG 5 cut(s) 134, 267, 370, 467, 529
TaaI ACNGT 2 cut(s) 753, 786
TaiI ACGT 5 cut(s) 40, 120, 177, 249, 893
TaqI TCGA 1 cut(s) 327
TasI AATT 1 cut(s) 684
TauI GCSGC 3 cut(s) 299, 434, 573
Tru1I TTAA 2 cut(s) 578, 665
Tru9I TTAA 2 cut(s) 578, 665
TscAI CASTG 4 cut(s) 388, 466, 813, 922
TseFI GTSAC 4 cut(s) 80, 625, 724, 891
TseI GCWGC 2 cut(s) 76, 407
Tsp45I GTSAC 4 cut(s) 80, 625, 724, 891
TspGWI ACGGA 4 cut(s) 272, 343, 666, 753
TspRI CASTG 4 cut(s) 388, 466, 813, 922
Van91I CCANNNNNTGG 2 cut(s) 553, 917
VneI GTGCAC 1 cut(s) 609
VpaK11BI GGWCC 1 cut(s) 131
VspI ATTAAT 1 cut(s) 665
XagI CCTNNNNNAGG 1 cut(s) 408
XceI RCATGY 1 cut(s) 775
XcmI CCANNNNNNNNNTGG 2 cut(s) 307, 484
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 3 cut(s) 26, 743, 864
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.