Rmu_sc0005467.1_g000012

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005467.1
OG0001358
Physical Location & Seq
Reverse (-)
56395 .. 60953
4559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005467.1_g000012.1.cds

Sequence Viewer

Length: 1005 bp
atggcggtgccggcgtacgaggcgactggcggagcggtgccggactggctgaacaaaggtgacaacgcatggcagatgatctccgccacgctggtgggtctccagagcgtgccggggctggtcatcctctacgggagcatagtgaagaagaagtgggcggtcaactccgccttcatggccctctatgccttcgccgccgtcgttctttgctgggtgttgtgggcctacaagatgtcgttcggggacgagctgctgccgttttgggggaaggccgggcctgccttagggcagaagtatctgctcaagcacgctgccctccccgaatccacacagcaccaccacgacggcagtattgagactcccatggccatgccgttatacccgatggcgaccatggtgtggttccagtgcgtgttcgcggccataacactgatactgttggcagggtcggtgctggggagaatgaacttcaaggcgtggatgatgttcgtgcctctgtggctgacgtcctcgtacaccgtcggtgcttttagcttgtggggcggagggttcttgttccactggggtgttatggattactccggcgggtacgtcattcatctttcttcagggattgctggtttcaccactgcttactgggtaggacctagggtgaagaaggacagggagagattcccaccaaacaacgtactactgacgttggccggagcagggttgctgtggttgggatgggccggtttcaacggcggagatccctacgcggccaacacagactcctccatggctgtcctcaacactaatatctgcgctgccacgagtctcttggtgtggacatggctcgatgtcatcttcttcaacaagccctccgtcatcggagctgtccagggaatgattactggcctcgtatgtattactcctgctgcaggttgctctttaacttttatcctcgtatatatgagactcaagactcttgatcatccattttccctaccattattaatttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

334

Amino Acids

36.37

Weight (kDa)

8.52

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 509
Acc36I ACCTGC 1 cut(s) 914
AccB1I GGYRCC 2 cut(s) 7, 37
AccB7I CCANNNNNTGG 1 cut(s) 399
AccBSI CCGCTC 1 cut(s) 35
AccII CGCG 2 cut(s) 419, 761
AclWI GGATC 1 cut(s) 746
AcoI YGGCCR 4 cut(s) 366, 420, 702, 762
AcuI CTGAAG 1 cut(s) 591
AcyI GRCGYC 1 cut(s) 506
AfaI GTAC 4 cut(s) 17, 515, 590, 690
AfiI CCNNNNNNNGG 5 cut(s) 263, 284, 399, 711, 923
AgsI TTSAA 3 cut(s) 472, 742, 856
AjnI CCWGG 1 cut(s) 882
AleI CACNNNNGTG 2 cut(s) 92, 564
AluBI AGCT 3 cut(s) 250, 534, 878
AluI AGCT 3 cut(s) 250, 534, 878
Alw26I GTCTC 4 cut(s) 104, 350, 824, 952
AlwI GGATC 1 cut(s) 746
ApeKI GCWGC 5 cut(s) 250, 253, 311, 809, 920
AseI ATTAAT 1 cut(s) 998
AspA2I CCTAGG 1 cut(s) 647
AspLEI GCGC 1 cut(s) 809
AspS9I GGNCC 5 cut(s) 178, 222, 275, 644, 732
AsuC2I CCSGG 2 cut(s) 114, 274
AsuHPI GGTGA 3 cut(s) 71, 616, 664
AvaII GGWCC 1 cut(s) 644
AvrII CCTAGG 1 cut(s) 647
AxyI CCTNAGG 1 cut(s) 283
BalI TGGCCA 1 cut(s) 368
BanI GGYRCC 2 cut(s) 7, 37
BauI CACGAG 1 cut(s) 814
BbvI GCAGC 5 cut(s) 237, 240, 298, 796, 907
BccI CCATC 2 cut(s) 379, 723
BceAI ACGGC 5 cut(s) 182, 241, 358, 361, 760
BciT130I CCWGG 1 cut(s) 884
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 2 cut(s) 114, 274
BcoDI GTCTC 4 cut(s) 104, 350, 824, 952
BfaI CTAG 1 cut(s) 648
BfmI CTRYAG 1 cut(s) 921
BfuAI ACCTGC 1 cut(s) 914
BglI GCCNNNNNGGC 3 cut(s) 46, 176, 499
BisI GCNGC 8 cut(s) 195, 251, 254, 312, 420, 762, 810, 921
BlnI CCTAGG 1 cut(s) 647
BlsI GCNGC 8 cut(s) 196, 252, 255, 313, 421, 763, 811, 922
Bme1390I CCNGG 3 cut(s) 114, 274, 884
Bme18I GGWCC 1 cut(s) 644
BmgT120I GGNCC 5 cut(s) 178, 222, 275, 644, 732
BmiI GGNNCC 3 cut(s) 9, 39, 404
BmrFI CCNGG 3 cut(s) 114, 274, 884
BmrI ACTGGG 2 cut(s) 571, 646
BmuI ACTGGG 2 cut(s) 571, 646
BpmI CTGGAG 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 287, 947
BpuMI CCSGG 2 cut(s) 114, 274
BsaHI GRCGYC 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 6 cut(s) 113, 363, 393, 647, 780, 883
BsaXI ACNNNNNCTCC 4 cut(s) 758, 788, 848, 878
Bsc4I CCNNNNNNNGG 5 cut(s) 263, 284, 399, 711, 923
Bse118I RCCGGY 2 cut(s) 10, 734
Bse1I ACTGG 6 cut(s) 31, 50, 406, 566, 641, 901
Bse21I CCTNAGG 1 cut(s) 283
BseBI CCWGG 1 cut(s) 884
BseDI CCNNGG 6 cut(s) 113, 363, 393, 647, 780, 883
BseGI GGATG 4 cut(s) 123, 486, 734, 976
BseLI CCNNNNNNNGG 5 cut(s) 263, 284, 399, 711, 923
BseNI ACTGG 6 cut(s) 31, 50, 406, 566, 641, 901
BseRI GAGGAG 1 cut(s) 766
BseXI GCAGC 5 cut(s) 237, 240, 298, 796, 907
BseYI CCCAGC 2 cut(s) 210, 454
Bsh1236I CGCG 2 cut(s) 419, 761
BshNI GGYRCC 2 cut(s) 7, 37
BsiSI CCGG 7 cut(s) 11, 41, 113, 273, 582, 705, 735
BsiWI CGTACG 1 cut(s) 15
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 5 cut(s) 263, 284, 399, 711, 923
BsmAI GTCTC 4 cut(s) 104, 350, 824, 952
BsmFI GGGAC 1 cut(s) 257
Bso31I GGTCTC 1 cut(s) 104
Bsp143I GATC 3 cut(s) 78, 751, 973
Bsp19I CCATGG 3 cut(s) 363, 393, 780
BspFNI CGCG 2 cut(s) 419, 761
BspLI GGNNCC 3 cut(s) 9, 39, 404
BspMAI CTGCAG 1 cut(s) 925
BspMI ACCTGC 1 cut(s) 914
BspPI GGATC 1 cut(s) 746
BspT107I GGYRCC 2 cut(s) 7, 37
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 35
BsrFI RCCGGY 2 cut(s) 10, 734
BsrI ACTGG 6 cut(s) 31, 50, 406, 566, 641, 901
BssAI RCCGGY 2 cut(s) 10, 734
BssECI CCNNGG 6 cut(s) 113, 363, 393, 647, 780, 883
BssMI GATC 3 cut(s) 78, 751, 973
BssNI GRCGYC 1 cut(s) 506
BssSI CACGAG 1 cut(s) 814
BssT1I CCWWGG 4 cut(s) 363, 393, 647, 780
Bst2BI CACGAG 1 cut(s) 814
Bst2UI CCWGG 1 cut(s) 884
Bst4CI ACNGT 2 cut(s) 438, 520
BstACI GRCGYC 1 cut(s) 506
BstC8I GCNNGC 4 cut(s) 12, 110, 279, 309
BstDEI CTNAG 1 cut(s) 283
BstDSI CCRYGG 3 cut(s) 363, 393, 780
BstENI CCTNNNNNAGG 2 cut(s) 282, 921
BstF5I GGATG 4 cut(s) 123, 486, 734, 976
BstFNI CGCG 2 cut(s) 419, 761
BstHHI GCGC 1 cut(s) 809
BstKTI GATC 3 cut(s) 81, 754, 976
BstMAI GTCTC 4 cut(s) 104, 350, 824, 952
BstMBI GATC 3 cut(s) 78, 751, 973
BstMWI GCNNNNNNNGC 9 cut(s) 11, 20, 46, 176, 185, 194, 278, 499, 540
BstNI CCWGG 1 cut(s) 884
BstSCI CCNGG 3 cut(s) 112, 272, 882
BstSFI CTRYAG 1 cut(s) 921
BstUI CGCG 2 cut(s) 419, 761
BstV1I GCAGC 5 cut(s) 237, 240, 298, 796, 907
BstX2I RGATCY 1 cut(s) 751
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 1 cut(s) 751
Bsu36I CCTNAGG 1 cut(s) 283
BtgI CCRYGG 3 cut(s) 363, 393, 780
BtsCI GGATG 4 cut(s) 123, 486, 734, 976
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 4 cut(s) 413, 428, 559, 627
BveI ACCTGC 1 cut(s) 914
Cac8I GCNNGC 4 cut(s) 12, 110, 279, 309
CfoI GCGC 1 cut(s) 809
Cfr10I RCCGGY 2 cut(s) 10, 734
Cfr13I GGNCC 5 cut(s) 178, 222, 275, 644, 732
Csp6I GTAC 4 cut(s) 16, 514, 589, 689
CviAII CATG 7 cut(s) 69, 175, 364, 370, 394, 781, 834
CviQI GTAC 4 cut(s) 16, 514, 589, 689
DdeI CTNAG 1 cut(s) 283
DpnI GATC 3 cut(s) 80, 753, 975
DpnII GATC 3 cut(s) 78, 751, 973
EaeI YGGCCR 4 cut(s) 366, 420, 702, 762
EciI GGCGGA 5 cut(s) 45, 73, 157, 558, 762
Eco130I CCWWGG 4 cut(s) 363, 393, 647, 780
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 644
Eco57I CTGAAG 1 cut(s) 591
Eco81I CCTNAGG 1 cut(s) 283
EcoNI CCTNNNNNAGG 2 cut(s) 282, 921
EcoO109I RGGNCCY 1 cut(s) 644
EcoRII CCWGG 1 cut(s) 882
EcoT14I CCWWGG 4 cut(s) 363, 393, 647, 780
ErhI CCWWGG 4 cut(s) 363, 393, 647, 780
FaeI CATG 7 cut(s) 72, 178, 367, 373, 397, 784, 837
FaqI GGGAC 1 cut(s) 257
FatI CATG 7 cut(s) 68, 174, 363, 369, 393, 780, 833
FauI CCCGC 1 cut(s) 578
FbaI TGATCA 1 cut(s) 973
Fnu4HI GCNGC 8 cut(s) 195, 251, 254, 312, 420, 762, 810, 921
FokI GGATG 4 cut(s) 110, 493, 741, 963
Fsp4HI GCNGC 8 cut(s) 195, 251, 254, 312, 420, 762, 810, 921
FspBI CTAG 1 cut(s) 648
GlaI GCGC 1 cut(s) 808
GluI GCNGC 8 cut(s) 195, 251, 254, 312, 420, 762, 810, 921
GsaI CCCAGC 2 cut(s) 214, 458
GsuI CTGGAG 1 cut(s) 86
HapII CCGG 7 cut(s) 11, 41, 113, 273, 582, 705, 735
HhaI GCGC 1 cut(s) 809
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 7 cut(s) 72, 178, 367, 373, 397, 784, 837
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 7 cut(s) 323, 358, 672, 773, 817, 960, 967
HpaII CCGG 7 cut(s) 11, 41, 113, 273, 582, 705, 735
HphI GGTGA 3 cut(s) 71, 616, 664
Hpy166II GTNNAC 3 cut(s) 163, 516, 831
Hpy188I TCNGA 1 cut(s) 875
Hpy188III TCNNGA 3 cut(s) 103, 964, 971
Hpy8I GTNNAC 3 cut(s) 163, 516, 831
Hpy99I CGWCG 3 cut(s) 203, 347, 524
HpyAV CCTTC 4 cut(s) 181, 199, 262, 652
HpyCH4III ACNGT 2 cut(s) 438, 520
HpyCH4IV ACGT 4 cut(s) 506, 591, 687, 698
HpyCH4V TGCA 1 cut(s) 923
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 20, 46, 176, 185, 194, 278, 499, 540
HpyF3I CTNAG 1 cut(s) 283
HpySE526I ACGT 4 cut(s) 506, 591, 687, 698
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 7 cut(s) 72, 178, 367, 373, 397, 784, 837
HspAI GCGC 1 cut(s) 807
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 3 cut(s) 78, 751, 973
LmnI GCTCC 4 cut(s) 32, 135, 707, 875
Lsp1109I GCAGC 5 cut(s) 237, 240, 298, 796, 907
MaeI CTAG 1 cut(s) 648
MaeII ACGT 4 cut(s) 506, 591, 687, 698
MaeIII GTNAC 1 cut(s) 59
MalI GATC 3 cut(s) 80, 753, 975
MbiI CCGCTC 1 cut(s) 35
MboI GATC 3 cut(s) 78, 751, 973
MboII GAAGA 6 cut(s) 157, 160, 597, 667, 841, 844
MflI RGATCY 1 cut(s) 751
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 1 cut(s) 999
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 5 cut(s) 352, 767, 826, 954, 961
Mox20I TGGCCA 1 cut(s) 368
MroNI GCCGGC 1 cut(s) 10
MscI TGGCCA 1 cut(s) 368
MseI TTAA 2 cut(s) 935, 998
MslI CAYNNNNRTG 3 cut(s) 92, 368, 564
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 7 cut(s) 11, 41, 113, 273, 582, 705, 735
MspR9I CCNGG 3 cut(s) 114, 274, 884
MvaI CCWGG 1 cut(s) 884
MvnI CGCG 2 cut(s) 419, 761
MwoI GCNNNNNNNGC 9 cut(s) 11, 20, 46, 176, 185, 194, 278, 499, 540
NaeI GCCGGC 1 cut(s) 12
NciI CCSGG 2 cut(s) 114, 274
NcoI CCATGG 3 cut(s) 363, 393, 780
NdeII GATC 3 cut(s) 78, 751, 973
NgoMIV GCCGGC 1 cut(s) 10
NlaIII CATG 7 cut(s) 72, 178, 367, 373, 397, 784, 837
NlaIV GGNNCC 3 cut(s) 9, 39, 404
NmuCI GTSAC 1 cut(s) 59
OliI CACNNNNGTG 2 cut(s) 92, 564
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PdiI GCCGGC 1 cut(s) 12
PfeI GAWTC 2 cut(s) 323, 672
Pfl23II CGTACG 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 399
PkrI GCNGC 8 cut(s) 196, 252, 255, 313, 421, 763, 811, 922
PleI GAGTC 5 cut(s) 352, 767, 825, 954, 961
PpsI GAGTC 5 cut(s) 352, 767, 825, 954, 961
PpuMI RGGWCCY 1 cut(s) 644
PshBI ATTAAT 1 cut(s) 998
Psp5II RGGWCCY 1 cut(s) 644
Psp6I CCWGG 1 cut(s) 882
PspFI CCCAGC 2 cut(s) 210, 454
PspGI CCWGG 1 cut(s) 882
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 3 cut(s) 9, 39, 404
PspPI GGNCC 5 cut(s) 178, 222, 275, 644, 732
PspPPI RGGWCCY 1 cut(s) 644
PstI CTGCAG 1 cut(s) 925
PsuI RGATCY 1 cut(s) 751
RsaI GTAC 4 cut(s) 17, 515, 590, 690
RsaNI GTAC 4 cut(s) 16, 514, 589, 689
RseI CAYNNNNRTG 3 cut(s) 92, 368, 564
SaqAI TTAA 2 cut(s) 935, 998
SatI GCNGC 8 cut(s) 195, 251, 254, 312, 420, 762, 810, 921
Sau3AI GATC 3 cut(s) 78, 751, 973
Sau96I GGNCC 5 cut(s) 178, 222, 275, 644, 732
SchI GAGTC 5 cut(s) 352, 767, 826, 954, 961
ScrFI CCNGG 3 cut(s) 114, 274, 884
SfcI CTRYAG 1 cut(s) 921
SinI GGWCC 1 cut(s) 644
SmiMI CAYNNNNRTG 3 cut(s) 92, 368, 564
SmlI CTYRAG 2 cut(s) 302, 962
SmoI CTYRAG 2 cut(s) 302, 962
Sse9I AATT 1 cut(s) 999
SspMI CTAG 1 cut(s) 648
StyD4I CCNGG 3 cut(s) 112, 272, 882
StyI CCWWGG 4 cut(s) 363, 393, 647, 780
TaaI ACNGT 2 cut(s) 438, 520
TaiI ACGT 4 cut(s) 509, 594, 690, 701
TaqI TCGA 1 cut(s) 840
TasI AATT 1 cut(s) 999
TauI GCSGC 3 cut(s) 197, 422, 764
TfiI GAWTC 2 cut(s) 323, 672
Tru1I TTAA 2 cut(s) 935, 998
Tru9I TTAA 2 cut(s) 935, 998
TscAI CASTG 4 cut(s) 413, 435, 566, 634
TseFI GTSAC 1 cut(s) 59
TseI GCWGC 5 cut(s) 250, 253, 311, 809, 920
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 3 cut(s) 163, 479, 587
TspGWI ACGGA 1 cut(s) 856
TspRI CASTG 4 cut(s) 413, 435, 566, 634
Van91I CCANNNNNTGG 1 cut(s) 399
VpaK11BI GGWCC 1 cut(s) 644
VspI ATTAAT 1 cut(s) 998
XagI CCTNNNNNAGG 2 cut(s) 282, 921
XcmI CCANNNNNNNNNTGG 1 cut(s) 820
XmaJI CCTAGG 1 cut(s) 647
XspI CTAG 1 cut(s) 648
ZraI GACGTC 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.